A. Forghani, A. H. Forghani, Maryam Altafi, Kazem Hashemi Majd, Omid Sofalian
Most of agricultural lands in Iran are located in arid and semi-arid regions and are considered as saline soils. In order to investigate the interaction of salinity as well as potassium and calcium on the growth and yield of tomato plants, a factorial experiment was perfected in the form of randomized complete blocks, in hydroponic conditions, with three replicates per treatment. Experimental factors include salinity at three levels (0, 20, and 40 mM NaCl), potassium content form chloride, nitrate (0 and 15 mM), and calcium from chloride, and nitrate (with 0 and 10 mM) . The studied growth factors, including plant height, stem diameter, number of leaves, flowers and fruits and leaf chlorophyll decreased Received 28.03.2021/ Revised 26.05.2021/ Accepted 27.06.2021/ Published 22.10.2021 :تفایرد 08 / 01 / 1400 / :حلاصا 05 / 03 / 1400 / :شریذپ 06 / 04 / 1400 / :راشتنا 30 / 07 / 1400 [ D O I: 1 0. 52 54 7/ nb r. 8. 3. 20 6 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .3 .5 .8 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 203 ]
伊朗的大部分农业用地位于干旱和半干旱地区，被认为是盐碱地。为了研究盐度以及钾和钙对番茄植株生长和产量的相互作用，在水培条件下，以随机完全块的形式完善了析因实验，每个处理重复三次。实验因素包括三个水平的盐度（0、20和40mM NaCl）、氯化物、硝酸盐（0和15mM）中的钾含量、氯化物中的钙以及硝酸盐（具有0和10mM）。所研究的生长因子，包括株高、茎径、叶数、花和果实以及叶片叶绿素下降。收到日期：2021年3月28日/修订日期：2021年5月26日接受日期：202年6月27日发布日期：2014年10月22日：2014年1月8日。52 54 7/nb r.8。3.20 6][日期：2.0。10 01.1.2 42 36 33 0。14 00.8.3.5.8][D ow nl oa de D fr om n br.k hu.a c.ir o n 20 21-1 203]
{"title":"The effects of different sources of potassium and calcium on yield and ionic balance of tomatoes under salinity stress in hydroponic cultivation","authors":"A. Forghani, A. H. Forghani, Maryam Altafi, Kazem Hashemi Majd, Omid Sofalian","doi":"10.52547/nbr.8.3.206","DOIUrl":"https://doi.org/10.52547/nbr.8.3.206","url":null,"abstract":"Most of agricultural lands in Iran are located in arid and semi-arid regions and are considered as saline soils. In order to investigate the interaction of salinity as well as potassium and calcium on the growth and yield of tomato plants, a factorial experiment was perfected in the form of randomized complete blocks, in hydroponic conditions, with three replicates per treatment. Experimental factors include salinity at three levels (0, 20, and 40 mM NaCl), potassium content form chloride, nitrate (0 and 15 mM), and calcium from chloride, and nitrate (with 0 and 10 mM) . The studied growth factors, including plant height, stem diameter, number of leaves, flowers and fruits and leaf chlorophyll decreased Received 28.03.2021/ Revised 26.05.2021/ Accepted 27.06.2021/ Published 22.10.2021 :تفایرد 08 / 01 / 1400 / :حلاصا 05 / 03 / 1400 / :شریذپ 06 / 04 / 1400 / :راشتنا 30 / 07 / 1400 [ D O I: 1 0. 52 54 7/ nb r. 8. 3. 20 6 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .3 .5 .8 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 203 ]","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49520618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gamma-decalactone, an intramolecular 4-hydroxydecanoic acid ester, has a peach-like aroma and is widely used in the food and cosmetics industries. The biotechnological production of this compound is possible via biotransformation of castor seed oil by the yeast Yarrowia lipolytica. This study aimed to compare the production of gamma-decalactone by wild-type strain with that in a mutant strain producing lipase in high amounts. It was found that cells with yeast-like morphology produce more gamma-decalactone than hyphae-like cells. The maximum production of gamma-decalactone by wild-type and mutant strains was 65 mg/L after 24h of inoculation and 90 mg/L after 72h of inoculation, respectively. The mutant strain converts 38% more substrate into gamma-decalactone than the wild-type strain, therefore, it could significantly increase the productivity of industrial-scale production of gamma-decalactone.
{"title":"A comparison of the production of gamma-decalactone in wild-type and mutant strains of Yarrowia lipolytica","authors":"F. Darvishi, Armin Kheirollahi Meidani","doi":"10.52547/nbr.8.3.183","DOIUrl":"https://doi.org/10.52547/nbr.8.3.183","url":null,"abstract":"Gamma-decalactone, an intramolecular 4-hydroxydecanoic acid ester, has a peach-like aroma and is widely used in the food and cosmetics industries. The biotechnological production of this compound is possible via biotransformation of castor seed oil by the yeast Yarrowia lipolytica. This study aimed to compare the production of gamma-decalactone by wild-type strain with that in a mutant strain producing lipase in high amounts. It was found that cells with yeast-like morphology produce more gamma-decalactone than hyphae-like cells. The maximum production of gamma-decalactone by wild-type and mutant strains was 65 mg/L after 24h of inoculation and 90 mg/L after 72h of inoculation, respectively. The mutant strain converts 38% more substrate into gamma-decalactone than the wild-type strain, therefore, it could significantly increase the productivity of industrial-scale production of gamma-decalactone.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44129209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
. Tamarix is the largest genus in the family Tamaricaceae with about 73 species. T. tetragyna var. meyeri and T. tetragyna var. deserti were previously reported from Iran. Due to the presence of overlapping features in the previously designed identification keys for the separation of these two varieties, it could be helpful to use other characters and character states derived from other sources, such as anatomical and molecular studies, in addition to find more effective morphological features for the separation of the two varieties. Therefore, the present study aimed to differentiate these two presumed varieties by multiple approaches using morphological, anatomical and molecular data. For morphological study, 6 quantitative and qualitative characters were examined in 12 samples of the two varieties. ANOVA analysis showed a significant difference between the studied characters. For anatomical study, 10 traits were examined in 4 samples of the two varieties, which also showed a significant difference. AMOVA analysis based on molecular studies using Scot marker showed a significant difference between the two varieties. The PCA biplot show the most variable traits in morphological and anatomical studies. For varieties delimitation, different clustering methods were drawn in all three studies. The results of this study showed that the use of appropriate diagnostic traits in morphological and anatomical studies as well as the use of molecular markers can be effective in showing the separated boundaries of the two varieties studied.
{"title":"A morphological, anatomical and molecular study of two varieties of Tamarix tetragyna in Iran","authors":"M. Veisi, F. Koohdar, M. Sheidai","doi":"10.52547/nbr.8.3.233","DOIUrl":"https://doi.org/10.52547/nbr.8.3.233","url":null,"abstract":". Tamarix is the largest genus in the family Tamaricaceae with about 73 species. T. tetragyna var. meyeri and T. tetragyna var. deserti were previously reported from Iran. Due to the presence of overlapping features in the previously designed identification keys for the separation of these two varieties, it could be helpful to use other characters and character states derived from other sources, such as anatomical and molecular studies, in addition to find more effective morphological features for the separation of the two varieties. Therefore, the present study aimed to differentiate these two presumed varieties by multiple approaches using morphological, anatomical and molecular data. For morphological study, 6 quantitative and qualitative characters were examined in 12 samples of the two varieties. ANOVA analysis showed a significant difference between the studied characters. For anatomical study, 10 traits were examined in 4 samples of the two varieties, which also showed a significant difference. AMOVA analysis based on molecular studies using Scot marker showed a significant difference between the two varieties. The PCA biplot show the most variable traits in morphological and anatomical studies. For varieties delimitation, different clustering methods were drawn in all three studies. The results of this study showed that the use of appropriate diagnostic traits in morphological and anatomical studies as well as the use of molecular markers can be effective in showing the separated boundaries of the two varieties studied.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47420025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomato (Solanum lycopersicum) is one of the most important crops and a known host of broomrapes. The species of genus Orobanche are biotic stress factors restricting plant growth and crop production. Some natural compounds and chemicals affect this biological relationship. Therefore, in the present study, the effect of gallic acid and kiwi fruit extract on the biological relationship between broomrape and tomato plant was investigated. To this end, first, the symbiotic effect of the broomrape species, Orobanche aegytica, on growth parameters of tomato plant and the amount of soluble sugar and photosynthetic pigments were evaluated. Then, the effect of concentrations of gallic acid and kiwi fruit extract at different levels on this biological relationship was investigated by measuring the growth parameters of tomato and broomrape, photosynthetic pigments and the amount of soluble sugar in tomatoes. The results demonstrated that the broomrapes reduced the fresh weight and dry weight of the stem, leaves and roots of tomato, diminished the leaf area of the host plant, and decreased the amount of photosynthetic pigments and its soluble sugar content. Gallic acid treatment and kiwi fruit extract lessened the growth parameters of the broomrape species and its overall growth, in addition to increasing the growth of tomato plants. This process could be associated with the constraint of the biological relationship between the parasite and the host, as the soluble sugar content as well as the amount of the photosynthetic pigments of tomato plants were increased and the tomato plants remained highly resistant.
{"title":"The effects of kiwi fruit extract and gallic acid on symbiotic relationship between broomrape and tomato","authors":"Asrin Arshi, A. Salimi, M. Chavoushi","doi":"10.52547/nbr.8.3.220","DOIUrl":"https://doi.org/10.52547/nbr.8.3.220","url":null,"abstract":"Tomato (Solanum lycopersicum) is one of the most important crops and a known host of broomrapes. The species of genus Orobanche are biotic stress factors restricting plant growth and crop production. Some natural compounds and chemicals affect this biological relationship. Therefore, in the present study, the effect of gallic acid and kiwi fruit extract on the biological relationship between broomrape and tomato plant was investigated. To this end, first, the symbiotic effect of the broomrape species, Orobanche aegytica, on growth parameters of tomato plant and the amount of soluble sugar and photosynthetic pigments were evaluated. Then, the effect of concentrations of gallic acid and kiwi fruit extract at different levels on this biological relationship was investigated by measuring the growth parameters of tomato and broomrape, photosynthetic pigments and the amount of soluble sugar in tomatoes. The results demonstrated that the broomrapes reduced the fresh weight and dry weight of the stem, leaves and roots of tomato, diminished the leaf area of the host plant, and decreased the amount of photosynthetic pigments and its soluble sugar content. Gallic acid treatment and kiwi fruit extract lessened the growth parameters of the broomrape species and its overall growth, in addition to increasing the growth of tomato plants. This process could be associated with the constraint of the biological relationship between the parasite and the host, as the soluble sugar content as well as the amount of the photosynthetic pigments of tomato plants were increased and the tomato plants remained highly resistant.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46858491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
. One of the major abiotic stresses that negatively affects plants is the presence of heavy metals. Soil pollution with heavy metals, resulting from the industrial development and use of fertilizers containing heavy metals, has become a major environmental concern in human societies. Mercury is a toxic heavy metal that causes pollution in agricultural lands. Accumulation of Hg by plants may disrupt many cellular functions and block growth and development. Under such conditions, the enzymatic and non-enzymatic defense systems of plants are activated. Several defense systems are cooperating together in plants to cope with stressful situations. In this study, the effect of different concentrations of mercury on the photosynthetic pigments content and non-enzymatic defence systems in Nicotiana tabacum was studied. After planting the plants under the same conditions in the hydroponic medium and feeding the plants with Hoagland solution, treatments with different concentrations of mercury nitrate (0.5, 1 and 3 mM and a control group) were applied to the seedlings in three replications. Ten days after the application of the treatments, the plants were harvested and examined. The results showed that tobacco plants which had been exposed to heavy metal used the accumulation of osmolytes such as proline and soluble sugars in order to balance their osmotic pressure. The decrease in the amounts of photosynthetic pigments and increase in the levels of malondialdehyde in the leaves indicated the elevation of oxidative damage. Increased activity of non-enzymatic antioxidants in tobacco leaves, including anthocyanins, phenol, flavonols and flavonoids, can be interpreted as the mechanisms of resistance to heavy metal stress induced by mercury.
{"title":"The effects of heavy metal mercury on some physiological responsses in Nicotiana tabacum","authors":"M. Farjadi, Akbar Norastehnia","doi":"10.52547/nbr.8.2.118","DOIUrl":"https://doi.org/10.52547/nbr.8.2.118","url":null,"abstract":". One of the major abiotic stresses that negatively affects plants is the presence of heavy metals. Soil pollution with heavy metals, resulting from the industrial development and use of fertilizers containing heavy metals, has become a major environmental concern in human societies. Mercury is a toxic heavy metal that causes pollution in agricultural lands. Accumulation of Hg by plants may disrupt many cellular functions and block growth and development. Under such conditions, the enzymatic and non-enzymatic defense systems of plants are activated. Several defense systems are cooperating together in plants to cope with stressful situations. In this study, the effect of different concentrations of mercury on the photosynthetic pigments content and non-enzymatic defence systems in Nicotiana tabacum was studied. After planting the plants under the same conditions in the hydroponic medium and feeding the plants with Hoagland solution, treatments with different concentrations of mercury nitrate (0.5, 1 and 3 mM and a control group) were applied to the seedlings in three replications. Ten days after the application of the treatments, the plants were harvested and examined. The results showed that tobacco plants which had been exposed to heavy metal used the accumulation of osmolytes such as proline and soluble sugars in order to balance their osmotic pressure. The decrease in the amounts of photosynthetic pigments and increase in the levels of malondialdehyde in the leaves indicated the elevation of oxidative damage. Increased activity of non-enzymatic antioxidants in tobacco leaves, including anthocyanins, phenol, flavonols and flavonoids, can be interpreted as the mechanisms of resistance to heavy metal stress induced by mercury.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46129043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Khanhasani, A. Jalili, Y. Khodakarami, N. Jalilian
Distribution of rivers, pools and grasslands, being large or small and perennial or temporary, have created conditions suitable for the establishment of wetlands, especially in the highland regions of the Country. These habitats are highly important for the preservation of biodiversity and other ecological aspects. The present study aimed to identify wetlands in the Kermanshah Province, Iran, and map their distribution. The investigation was carried out on the basis of the conventional taxonomic studies of the region by doing field observation in 31 aqueous habitats in Kermanshah Province. The flora of each habitats and the life form and geographical distribution of the species were investigated. In total, 617 plant specimens, belonging to 288 species, 218 genera and 62 families, were collected identified and stored in the herbarium of the research center of agriculture and natural resources of Kermanshah Province (RANK). Asteraceae with 41 species, Poaceae with 31 species, Fabaceae with 31 species and Brassicaceae with 24 species were found to be the most important families of the wetlands studied. Trifolium with 11 species and Bromus with 6 species were the most important genera, in terms of species richness. Detection of the biological forms showed that 40.3% of the species were trophytes, 35/5% were hemicryptophyts, 6/1% were phanerophytes, 14/23% were cryptophytes and 3/72% were chamaephytes. The chorology of species showed that 93 species belonged to more than two elements (32%), 92 species (31%) to the Irano-Touranin elements, 32 species to Irano-TouraninMediterranean (11%), 9 species to Irano-TouraninEuro-Siberian with (3%) and 23 species were found to be cosmopolitan elements (8%).
{"title":"Wetland flora of Kermanshah Province, Iran","authors":"M. Khanhasani, A. Jalili, Y. Khodakarami, N. Jalilian","doi":"10.52547/nbr.8.2.154","DOIUrl":"https://doi.org/10.52547/nbr.8.2.154","url":null,"abstract":"Distribution of rivers, pools and grasslands, being large or small and perennial or temporary, have created conditions suitable for the establishment of wetlands, especially in the highland regions of the Country. These habitats are highly important for the preservation of biodiversity and other ecological aspects. The present study aimed to identify wetlands in the Kermanshah Province, Iran, and map their distribution. The investigation was carried out on the basis of the conventional taxonomic studies of the region by doing field observation in 31 aqueous habitats in Kermanshah Province. The flora of each habitats and the life form and geographical distribution of the species were investigated. In total, 617 plant specimens, belonging to 288 species, 218 genera and 62 families, were collected identified and stored in the herbarium of the research center of agriculture and natural resources of Kermanshah Province (RANK). Asteraceae with 41 species, Poaceae with 31 species, Fabaceae with 31 species and Brassicaceae with 24 species were found to be the most important families of the wetlands studied. Trifolium with 11 species and Bromus with 6 species were the most important genera, in terms of species richness. Detection of the biological forms showed that 40.3% of the species were trophytes, 35/5% were hemicryptophyts, 6/1% were phanerophytes, 14/23% were cryptophytes and 3/72% were chamaephytes. The chorology of species showed that 93 species belonged to more than two elements (32%), 92 species (31%) to the Irano-Touranin elements, 32 species to Irano-TouraninMediterranean (11%), 9 species to Irano-TouraninEuro-Siberian with (3%) and 23 species were found to be cosmopolitan elements (8%).","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70689776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
. Centaurea glastifolia , a plant species from the Asteraceae family, is distributed in the Northwest of Iran. This study carried out to determine suitable habitats and habitat connectivity of Centaurea glastifolia in three Provinces of West Azarbayejan, East Azarbayejan and Ardabil. Seven habitat variables and 36 presence points were selected for habitat modelling using six habitat distribution models of GLM, GAM, MARS, MaxEnt, RF and GBM. The ensemble map resulting from these models was used for habitat connectivity modelling using electrical-circuit method. Results of habitat evaluation revealed that suitable habitats of the species studied were mainly located in West Azarbayejan and east of Ardabil Provinces, for which distance from agricultural lands, mean annual temperature and distance from rangelands had the highest contribution to the model. Habitat connectivity modelling showed that the current density movement was highest in West Azarbayejan. Current density movement between East and Southeast of Ardabil was evaluated to be significant, whereas the current density movement between West Azarbayejan and Ardabil Provinces was relatively low. The high current density movement in West Azarbayejan indicates the species’ high dispersal ability in expanding its range across the area. The results of the current study could facilitate understanding of the distribution and dispersal of Centaurea glastifolia .
{"title":"Habitat distribution and connectivity modelling of Centaurea glastifolia L. in northwest of Iran","authors":"K. Almasieh, Kazem Negaresh, M. Mahmoodi","doi":"10.52547/nbr.8.2.142","DOIUrl":"https://doi.org/10.52547/nbr.8.2.142","url":null,"abstract":". Centaurea glastifolia , a plant species from the Asteraceae family, is distributed in the Northwest of Iran. This study carried out to determine suitable habitats and habitat connectivity of Centaurea glastifolia in three Provinces of West Azarbayejan, East Azarbayejan and Ardabil. Seven habitat variables and 36 presence points were selected for habitat modelling using six habitat distribution models of GLM, GAM, MARS, MaxEnt, RF and GBM. The ensemble map resulting from these models was used for habitat connectivity modelling using electrical-circuit method. Results of habitat evaluation revealed that suitable habitats of the species studied were mainly located in West Azarbayejan and east of Ardabil Provinces, for which distance from agricultural lands, mean annual temperature and distance from rangelands had the highest contribution to the model. Habitat connectivity modelling showed that the current density movement was highest in West Azarbayejan. Current density movement between East and Southeast of Ardabil was evaluated to be significant, whereas the current density movement between West Azarbayejan and Ardabil Provinces was relatively low. The high current density movement in West Azarbayejan indicates the species’ high dispersal ability in expanding its range across the area. The results of the current study could facilitate understanding of the distribution and dispersal of Centaurea glastifolia .","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47269824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
alireza Amini Hajiabadi, A. Mosleh Arani, Someh Ghasemi, M. H. Rad, Shima Shabazi, H. Etesami
. Salinity stress is an important challenge for wheat production in the world. Plant growth promoting rhizosphere bacteria, isolated from halophytic plants, can increase the tolerance of crop plants to salinity by direct and indirect mechanisms. In this study, plant growth-promoting traits of bacterial strains ( Bacillus safensis , Bacillus pumilus and Zhihengliuella halotolerans ), isolated from the rhizosphere of several halophyte plants, were deterimined and their effects on some vegetative traits and ionic content of wheat plant irrigated with saline water ( 0.2, as control, 4, 8 and 16 dS/m) were measured. Result showed that all three bacteria were able to produce auxin, hydrogen cyanide, siderophore, 1-aminocyclopropane-1-carboxylic acid deaminase and soluble phosphate. The increase in salinity levels caused increase in the concentration of sodium and decrease in the concentration of potassium, calcium and phosphorus in wheat leaves, as well as decrease in stem length, shoot and root dry weight, root to shoot dry weight ratio and total biomass. In wheat plants irrigated with saline water and inoculated with the bacterial strains, sodium concentration decreased up to 17.7% and concentrations of potassium, calcium, phosphorus and potassium to sodium ratio increased up to 33, 25.7, 200.4 and 41%, respectively. The most efficient bacterium was found to be Z. halotolerans . All bacterial isolates also increased stem length, shoot and root dry weight, root to shoot dry weight ratio and total biomass by 17, 58.6, 137, 88 and 66 %, respectively. The results of this study showed that the plant growth-promoting bacteria of rangeland halophytic plants potentionally improve the growth indices of wheat plants in saline conditions. These results also showed that the rhizosphere of halophytic plants in rangelands can be a good source for the isolation of salinity-resistant bacteria to improve the resistance of wheat plants to salinity.
{"title":"The effect of plant growth promoting potentials of rhizosphere bacteria isolated from several halophytic species on vegetative growth and ionic content of wheat","authors":"alireza Amini Hajiabadi, A. Mosleh Arani, Someh Ghasemi, M. H. Rad, Shima Shabazi, H. Etesami","doi":"10.52547/nbr.8.2.104","DOIUrl":"https://doi.org/10.52547/nbr.8.2.104","url":null,"abstract":". Salinity stress is an important challenge for wheat production in the world. Plant growth promoting rhizosphere bacteria, isolated from halophytic plants, can increase the tolerance of crop plants to salinity by direct and indirect mechanisms. In this study, plant growth-promoting traits of bacterial strains ( Bacillus safensis , Bacillus pumilus and Zhihengliuella halotolerans ), isolated from the rhizosphere of several halophyte plants, were deterimined and their effects on some vegetative traits and ionic content of wheat plant irrigated with saline water ( 0.2, as control, 4, 8 and 16 dS/m) were measured. Result showed that all three bacteria were able to produce auxin, hydrogen cyanide, siderophore, 1-aminocyclopropane-1-carboxylic acid deaminase and soluble phosphate. The increase in salinity levels caused increase in the concentration of sodium and decrease in the concentration of potassium, calcium and phosphorus in wheat leaves, as well as decrease in stem length, shoot and root dry weight, root to shoot dry weight ratio and total biomass. In wheat plants irrigated with saline water and inoculated with the bacterial strains, sodium concentration decreased up to 17.7% and concentrations of potassium, calcium, phosphorus and potassium to sodium ratio increased up to 33, 25.7, 200.4 and 41%, respectively. The most efficient bacterium was found to be Z. halotolerans . All bacterial isolates also increased stem length, shoot and root dry weight, root to shoot dry weight ratio and total biomass by 17, 58.6, 137, 88 and 66 %, respectively. The results of this study showed that the plant growth-promoting bacteria of rangeland halophytic plants potentionally improve the growth indices of wheat plants in saline conditions. These results also showed that the rhizosphere of halophytic plants in rangelands can be a good source for the isolation of salinity-resistant bacteria to improve the resistance of wheat plants to salinity.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45514145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ghiasvand, A. Makhdoumi, Maryam Moghaddam Matin, J. Vaezi
Regarding the rising rate of esophageal cancer in some parts of Asia, including northern and northeastern regions of Iran, the identification of new anti-cancer compounds is essential to combat the disease. The relation between some plants metabolites and these endophytic microorganisms are well characterized. In the current study, the potentials of Ephedra endophyte bacteria for the inhibition of esophageal cancer cell line (KYSE-30) proliferation were investigated. A total of 54 endophyte bacteria (out of 70) were obtained from the sterilized surfaces of two medicinal plants, i.e., Ephedra intermedia and Ephedra foliata. Bacterial strains were then cultured in Tryptic soy broth (TSB) medium and, after 72 h incubation, the produced secondary metabolites were extracted by chloroform. Anticancer effects of secondary metabolites from theses bacteria on esophageal cancer cell line KYSE-30 were evaluated after 24, 48 and 72 h by MTT method. MTT assay results showed that only strain A1 had a cytotoxic effect on KYSE-30 cells. The IC50 amounts of this strain against KYSE-30 cell lines were equaled (μg/ml) to 346.4, 192.8 and 121.3 after 24, 48, and 72 hours, respectively. The molecular identification of strain A1 revealed that Microbacterium maritypicum (99.8% Received 09.07.2020/ Revised 31.08.2020/ Accepted 19.11.2020/ Published 01.07.2021 :تفايرد ١٩ / ٤ ٠ / ٩ ١٣٩ / :حلاصا ٠ ١ / ٦ ٠ / ٩ ١٣٩ / پ :شريذ ٢٩ / ٨ ٠ / ٩ ١٣٩ / :راشتنا ١٠ / ٤ ٠ / ٤٠٠ ١ [ D O I: 1 0. 52 54 7/ nb r. 8. 2. 95 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .2 .1 .2 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 124 ]
关于亚洲一些地区，包括伊朗北部和东北部地区食管癌症发病率的上升，识别新的抗癌化合物对于对抗这种疾病至关重要。一些植物代谢产物与这些内生微生物之间的关系得到了很好的表征。在本研究中，研究了麻黄碱内生菌抑制食管癌症细胞系（KYSE-30）增殖的潜力。从中间麻黄和叶麻黄两种药用植物的无菌表面共获得54个内生细菌（70个）。然后在胰蛋白酶大豆肉汤（TSB）培养基中培养细菌菌株，培养72小时后，用氯仿提取产生的次级代谢产物。用MTT法分别于24、48和72小时后评价了这些细菌次级代谢产物对食管癌症细胞株KYSE-30的抗癌作用。MTT法检测结果表明，只有A1菌株对KYSE-30细胞具有细胞毒性作用。24、48和72小时后，该菌株对KYSE-30细胞系的IC50量分别为346.4、192.8和121.3（μg/ml）。菌株A1的分子鉴定显示，海洋型微杆菌（99.8%，2020年7月9日收到，2020年8月31日修订，2020年11月19日接受/2021年7月1日发布：تفايرد١٩/ٟداا٠/：ح；/٨٠/٩。52 54 7/nb r.8。2.95][D O R:20。10 01.1.2 42 36 33 0。14 00.8.2.1.2][D ow nl oa de D fr om n br.k hu.a c.ir o n 20 21-1 124]
{"title":"Inhibition of esophageal cancer cell line (KYSE-30) proliferation using secondary metabolites of Ephedra endophyte bacteria","authors":"M. Ghiasvand, A. Makhdoumi, Maryam Moghaddam Matin, J. Vaezi","doi":"10.52547/nbr.8.2.95","DOIUrl":"https://doi.org/10.52547/nbr.8.2.95","url":null,"abstract":"Regarding the rising rate of esophageal cancer in some parts of Asia, including northern and northeastern regions of Iran, the identification of new anti-cancer compounds is essential to combat the disease. The relation between some plants metabolites and these endophytic microorganisms are well characterized. In the current study, the potentials of Ephedra endophyte bacteria for the inhibition of esophageal cancer cell line (KYSE-30) proliferation were investigated. A total of 54 endophyte bacteria (out of 70) were obtained from the sterilized surfaces of two medicinal plants, i.e., Ephedra intermedia and Ephedra foliata. Bacterial strains were then cultured in Tryptic soy broth (TSB) medium and, after 72 h incubation, the produced secondary metabolites were extracted by chloroform. Anticancer effects of secondary metabolites from theses bacteria on esophageal cancer cell line KYSE-30 were evaluated after 24, 48 and 72 h by MTT method. MTT assay results showed that only strain A1 had a cytotoxic effect on KYSE-30 cells. The IC50 amounts of this strain against KYSE-30 cell lines were equaled (μg/ml) to 346.4, 192.8 and 121.3 after 24, 48, and 72 hours, respectively. The molecular identification of strain A1 revealed that Microbacterium maritypicum (99.8% Received 09.07.2020/ Revised 31.08.2020/ Accepted 19.11.2020/ Published 01.07.2021 :تفايرد ١٩ / ٤ ٠ / ٩ ١٣٩ / :حلاصا ٠ ١ / ٦ ٠ / ٩ ١٣٩ / پ :شريذ ٢٩ / ٨ ٠ / ٩ ١٣٩ / :راشتنا ١٠ / ٤ ٠ / ٤٠٠ ١ [ D O I: 1 0. 52 54 7/ nb r. 8. 2. 95 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .2 .1 .2 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 124 ]","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48013090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leila Zarandi-Miandoab, N. Chaparzadeh, Hamid Fekri-Shali
. In order to investigate the effects of salinity and magnesium (Mg) on the growth parameters, physiological characteristics and contents of some metabolites in Syrian bean-caper ( Zygophyllum fabago ) plants, a factorial experiment with completely randomized design was performed and carried out in perlite with Hoagland solution. The treatments were combinations of two levels of salinity (0 and 300 mM NaCl) and three levels of Mg concentration (0, 2 and 6 mM excess to the standard Mg content of Hoagland solution). Salinity did not change the fresh weight of the plants, but application of Mg in the growth medium of plants increased the dry weight significantly. Salinity reduced the leaf area, but the presence of Mg improved and even increased the leaf area of the plants. The Mg reduced NAR, while increased LAR and RLGR. Salinity decreased the RLGR. The interaction of salinity and Mg increased and improved RGR, LWR, RLGR. The tolerance index in salinity treatments increased with the presence of Mg. The R/S ratio showed a significant increase only in salinity condition, however, Mg moderated this ratio. Salinity reduced the photosynthetic pigments, however, application of Mg largely alleviated this decrease. The interaction of salinity and Mg increased the total sugar content of the leaves and reduced the total sugar content of the roots. Salinity and Mg reduced the total proteins content of all the organs of the plants. In general, salinity had a negative effect on the physiological parameters of the Zygophyllum fabago plants, while the application of supplementary Mg improved the growth indices and increased the plants tolerance against salinity.
{"title":"The effects of magnesium on the growth and physiological characteristics of Syrian bean-caper (Zygophyllum fabago) in saline conditions","authors":"Leila Zarandi-Miandoab, N. Chaparzadeh, Hamid Fekri-Shali","doi":"10.52547/nbr.8.2.130","DOIUrl":"https://doi.org/10.52547/nbr.8.2.130","url":null,"abstract":". In order to investigate the effects of salinity and magnesium (Mg) on the growth parameters, physiological characteristics and contents of some metabolites in Syrian bean-caper ( Zygophyllum fabago ) plants, a factorial experiment with completely randomized design was performed and carried out in perlite with Hoagland solution. The treatments were combinations of two levels of salinity (0 and 300 mM NaCl) and three levels of Mg concentration (0, 2 and 6 mM excess to the standard Mg content of Hoagland solution). Salinity did not change the fresh weight of the plants, but application of Mg in the growth medium of plants increased the dry weight significantly. Salinity reduced the leaf area, but the presence of Mg improved and even increased the leaf area of the plants. The Mg reduced NAR, while increased LAR and RLGR. Salinity decreased the RLGR. The interaction of salinity and Mg increased and improved RGR, LWR, RLGR. The tolerance index in salinity treatments increased with the presence of Mg. The R/S ratio showed a significant increase only in salinity condition, however, Mg moderated this ratio. Salinity reduced the photosynthetic pigments, however, application of Mg largely alleviated this decrease. The interaction of salinity and Mg increased the total sugar content of the leaves and reduced the total sugar content of the roots. Salinity and Mg reduced the total proteins content of all the organs of the plants. In general, salinity had a negative effect on the physiological parameters of the Zygophyllum fabago plants, while the application of supplementary Mg improved the growth indices and increased the plants tolerance against salinity.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49138373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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