Iraj Hashemzadeh, Seyedeh Narjes Tabatabaei, Nabiallah Ghaed Rahmati, M. Amiri, L. Bernatchez
The cave barb habitat is located in a Karst formation along the Sezar River. The springs on the walls of the Sezar River valley may provide a means for fish in surface waters to penetrate into the underground waters. These observations propose the probability for a migratory relationship between Garra gymnothorax in the Sezar River and the cave barbs (Garra typhlops and Garra lorestanensis). In addition, a variety of different body shapes including fusiform and slender body forms are observed among the cave fish. This phenotypical variation may be a sign of an unknown genetic diversity or could be attributed to the variable environmental conditions in different parts of the subterranean habitat. To clarify the situation, we used the sequences of mtDNA cytochrome oxidase subunit I and next generation sequencing method. The results showed that the fusiform and slender body shapes of G. typhlops and G. lorestanensis were not different with regard to their mtDNA and genomic compositions. Moreover, the analysis of the genomic Received 22.04.2018/ Revised 23.08.2018/ Accepted 03.09.2018/ Published 04.04.2020 :تفايرد ٠١ / ٢ ٠ / ٧ ١٣٩ / :حلاصا ٠١ / ٠٦ / ١٣٩٧ / :شريذپ ١٢ / ٠٦ / ١٣٩٧ / :راشتنا ١٦ / ٠١ / ٩ ١٣٩ D ow nl oa de d fr om n br .k hu .a c. ir at 9 :4 6 IR D T o n M on da y A pr il 6t h 20 20
{"title":"The analysis of the relationship between Lorestan cave barbs (Garra typhlops and Garra lorestanensis) and Garra gymnothorax populations in Dez and Karkheh River drainages","authors":"Iraj Hashemzadeh, Seyedeh Narjes Tabatabaei, Nabiallah Ghaed Rahmati, M. Amiri, L. Bernatchez","doi":"10.29252/NBR.7.1.1","DOIUrl":"https://doi.org/10.29252/NBR.7.1.1","url":null,"abstract":"The cave barb habitat is located in a Karst formation along the Sezar River. The springs on the walls of the Sezar River valley may provide a means for fish in surface waters to penetrate into the underground waters. These observations propose the probability for a migratory relationship between Garra gymnothorax in the Sezar River and the cave barbs (Garra typhlops and Garra lorestanensis). In addition, a variety of different body shapes including fusiform and slender body forms are observed among the cave fish. This phenotypical variation may be a sign of an unknown genetic diversity or could be attributed to the variable environmental conditions in different parts of the subterranean habitat. To clarify the situation, we used the sequences of mtDNA cytochrome oxidase subunit I and next generation sequencing method. The results showed that the fusiform and slender body shapes of G. typhlops and G. lorestanensis were not different with regard to their mtDNA and genomic compositions. Moreover, the analysis of the genomic Received 22.04.2018/ Revised 23.08.2018/ Accepted 03.09.2018/ Published 04.04.2020 :تفايرد ٠١ / ٢ ٠ / ٧ ١٣٩ / :حلاصا ٠١ / ٠٦ / ١٣٩٧ / :شريذپ ١٢ / ٠٦ / ١٣٩٧ / :راشتنا ١٦ / ٠١ / ٩ ١٣٩ D ow nl oa de d fr om n br .k hu .a c. ir at 9 :4 6 IR D T o n M on da y A pr il 6t h 20 20","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41770443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilnaz Abolhasani, J. Baharara, N. Shahri, E. Amini
In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star (Ophiocoma erinaceus) on male Wistar rat skin wound healing. In this experimental study, 60 male Wistar rats were divided randomly into 5 groups including control, positive control (treatment with honey), experimental 1 to 3 (treatment with 12.5, 25, 37.5 mg/kg body weight of extracted polysaccharide, respectively). In all groups, the wound was inflicted in the posterior part of the body of rats. Then, rats were treated locally. On the 3th, 7th, 10th and 14th days, samples were collected from the healing hole and histological changes were investigated by light microscopy. Then, quantitative data were analyzed by SPSS software, one-way ANOVA at the level of p<0.05. Microscopic results showed that, in treated wounds by 37.5 mg/kg of extracted polysaccharide, as well as Received 18.06.2018/ Revised 26.07.2019/ Accepted 11.11.2019/ Published 04.04.2020 :تفايرد ٢٨ / ٣ ٠ / ٧ ١٣٩ / :حلاصا ٠٤ / ٠٥ / ٨ ١٣٩ / :شريذپ ٠ ٢ / ٠٨ / ٨ ١٣٩ / :راشتنا ١٦ / ٠١ / ٩ ١٣٩ D ow nl oa de d fr om n br .k hu .a c. ir at 9 :4 6 IR D T o n M on da y A pr il 6t h 20 20
{"title":"The regenerative properties of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on cutaneous wound in male Wistar rat","authors":"Ilnaz Abolhasani, J. Baharara, N. Shahri, E. Amini","doi":"10.29252/NBR.7.1.9","DOIUrl":"https://doi.org/10.29252/NBR.7.1.9","url":null,"abstract":"In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star (Ophiocoma erinaceus) on male Wistar rat skin wound healing. In this experimental study, 60 male Wistar rats were divided randomly into 5 groups including control, positive control (treatment with honey), experimental 1 to 3 (treatment with 12.5, 25, 37.5 mg/kg body weight of extracted polysaccharide, respectively). In all groups, the wound was inflicted in the posterior part of the body of rats. Then, rats were treated locally. On the 3th, 7th, 10th and 14th days, samples were collected from the healing hole and histological changes were investigated by light microscopy. Then, quantitative data were analyzed by SPSS software, one-way ANOVA at the level of p<0.05. Microscopic results showed that, in treated wounds by 37.5 mg/kg of extracted polysaccharide, as well as Received 18.06.2018/ Revised 26.07.2019/ Accepted 11.11.2019/ Published 04.04.2020 :تفايرد ٢٨ / ٣ ٠ / ٧ ١٣٩ / :حلاصا ٠٤ / ٠٥ / ٨ ١٣٩ / :شريذپ ٠ ٢ / ٠٨ / ٨ ١٣٩ / :راشتنا ١٦ / ٠١ / ٩ ١٣٩ D ow nl oa de d fr om n br .k hu .a c. ir at 9 :4 6 IR D T o n M on da y A pr il 6t h 20 20","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48069575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Charcoal rot caused by Macrophomina phaseolina is one of the most important soil borne diseases with a broad host range including bean, which annually brings a significant damage to this plant. Biological control of charcoal rot is very important because its chemical control harms the environment, microflora and soil fertility. Chemical control of charcoal rot is also difficult and sometimes ineffective. Fluorescent Pseudomonads are able to increase plant growth and inhibit the development of plant pathogens by producing and secreting antibiotics, enzymes, siderophores, and plant hormones. In this study, infected bean plants by M. phaseolina were collected from infected bean fields of Khorramabad (Lorestan Province, Iran) in the summer of 2015. Virulence of fungal isolates was evaluated in a greenhouse and one isolate with the highest pathogenicity was chosen for further experiments. The biocontrol potential of Received 29.01.2018/ Revised 27.03.2018/ Accepted 30.03.2018/ Published 04.04.2020 :تفايرد ٩ ١ / ١١ / ٧ ١٣٩ / :حلاصا ٠٧ / ١ ٠ / ٨ ١٣٩ / :شريذپ ١٠ / ٠٢ / ٨ ١٣٩ / :راشتنا ١٦ / ٠٢ / ٩ ١٣٩ D ow nl oa de d fr om n br .k hu .a c. ir at 2 :1 8 IR D T o n T ue sd ay A ug us t 2 5t h 20 20 .ناراكمه و ساپ چراق يتسيز راهم Macrophomina سانومودوس يرتكاب طسوت Pas et al. The biocontrol potential of Pseudomonas against Macrophomina 65 ٦٥ / eight Pseudomonas fluorescens strains, whose biocontrol abilities were proved in previous studies, was examined against M. phaseolina in vitro. The growth inhibition of M. phaseolina was examined by dual culture test and the antifungal activity of bacterial volatile and nonvolatile metabolites. P. fluorescens UTPf125, which showed the highest inhibitory effect on the mycelial growth, was selected for greenhouse tests. UTPf125 strain led to a significant reduction (%50) of disease severity and increased fresh and dry weight significantly. Phenol compounds were evaluated 1, 3, 5, 7 and 9 days after inoculation by pathogen. The results showed that the highest value of total phenol content was obtained on the third and fifth days after inoculation, decreasing on the seventh and ninth days.
{"title":"The biocontrol potential of Pseudomonas fluorescens against Macrophomina phaseolina and estimating the total phenol compounds of bean roots","authors":"M. Pas, H. Shahbazi, L. Ebrahimi","doi":"10.29252/NBR.7.1.64","DOIUrl":"https://doi.org/10.29252/NBR.7.1.64","url":null,"abstract":"Charcoal rot caused by Macrophomina phaseolina is one of the most important soil borne diseases with a broad host range including bean, which annually brings a significant damage to this plant. Biological control of charcoal rot is very important because its chemical control harms the environment, microflora and soil fertility. Chemical control of charcoal rot is also difficult and sometimes ineffective. Fluorescent Pseudomonads are able to increase plant growth and inhibit the development of plant pathogens by producing and secreting antibiotics, enzymes, siderophores, and plant hormones. In this study, infected bean plants by M. phaseolina were collected from infected bean fields of Khorramabad (Lorestan Province, Iran) in the summer of 2015. Virulence of fungal isolates was evaluated in a greenhouse and one isolate with the highest pathogenicity was chosen for further experiments. The biocontrol potential of Received 29.01.2018/ Revised 27.03.2018/ Accepted 30.03.2018/ Published 04.04.2020 :تفايرد ٩ ١ / ١١ / ٧ ١٣٩ / :حلاصا ٠٧ / ١ ٠ / ٨ ١٣٩ / :شريذپ ١٠ / ٠٢ / ٨ ١٣٩ / :راشتنا ١٦ / ٠٢ / ٩ ١٣٩ D ow nl oa de d fr om n br .k hu .a c. ir at 2 :1 8 IR D T o n T ue sd ay A ug us t 2 5t h 20 20 .ناراكمه و ساپ چراق يتسيز راهم Macrophomina سانومودوس يرتكاب طسوت Pas et al. The biocontrol potential of Pseudomonas against Macrophomina 65 ٦٥ / eight Pseudomonas fluorescens strains, whose biocontrol abilities were proved in previous studies, was examined against M. phaseolina in vitro. The growth inhibition of M. phaseolina was examined by dual culture test and the antifungal activity of bacterial volatile and nonvolatile metabolites. P. fluorescens UTPf125, which showed the highest inhibitory effect on the mycelial growth, was selected for greenhouse tests. UTPf125 strain led to a significant reduction (%50) of disease severity and increased fresh and dry weight significantly. Phenol compounds were evaluated 1, 3, 5, 7 and 9 days after inoculation by pathogen. The results showed that the highest value of total phenol content was obtained on the third and fifth days after inoculation, decreasing on the seventh and ninth days.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43228889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Hamzeh'ee, Mostafa Koshnevis, P. Ashouri, V. Mozaffarian, H. Ravanbakhsh
. Fire affects vegetation and changes plant succession. In this paper, the vegetation of Sirachal Research Station, which burned in the summer of 2014, was studied and compared with the unburnt areas, based on biodiversity indices. The research was implemented as a factorial experiment in a completely randomized design. First, on the basis of physiognomy, the area was divided into three parts: shrubland, shrubland-rangeland, and rangeland. Each part was, then, divided into two sections, including a burnt area and an adjacent unburnt area (control area). In each area (burnt and unburnt), three sampling units were randomly assigned using PNP method, and vegetation measurements were subsequently performed. Based on data analysis, a total of 141 taxa were identified, belonging to 28 families and 95 genera, including one species of Gymnosperm, 19 monocotyledons and 121 dicotyledons in two burnt and unburnt areas of Sirachal Station. Based on the statistical analysis, using Past3 and SPSS softwares, there was no significant difference in the number of taxa between the vegetation of the burnt and unburnt areas. Of the indicators analyzed in Past3, the number of individual, the index of Menhinick's richness and the Shannon diversity index were significantly different in the treatments sampled from the burnt and unburnt areas. According to the Duncan test average, the number of individuals in the unburnt area, with a canopy cover below 25%, was greater than that in the burnt area, with the same canopy cover, however, no significant difference was observed between the individuals in the burnt area and unburnt area, with the same canopy cover of more than 25%. Also, the richness of the burnt area with a canopy of less than 5% is significantly higher than that in the unburnt area with the same canopy cover.
{"title":"The effect of fire on vegetation diversity indices, a case study: Sirachal research station","authors":"B. Hamzeh'ee, Mostafa Koshnevis, P. Ashouri, V. Mozaffarian, H. Ravanbakhsh","doi":"10.29252/NBR.7.1.92","DOIUrl":"https://doi.org/10.29252/NBR.7.1.92","url":null,"abstract":". Fire affects vegetation and changes plant succession. In this paper, the vegetation of Sirachal Research Station, which burned in the summer of 2014, was studied and compared with the unburnt areas, based on biodiversity indices. The research was implemented as a factorial experiment in a completely randomized design. First, on the basis of physiognomy, the area was divided into three parts: shrubland, shrubland-rangeland, and rangeland. Each part was, then, divided into two sections, including a burnt area and an adjacent unburnt area (control area). In each area (burnt and unburnt), three sampling units were randomly assigned using PNP method, and vegetation measurements were subsequently performed. Based on data analysis, a total of 141 taxa were identified, belonging to 28 families and 95 genera, including one species of Gymnosperm, 19 monocotyledons and 121 dicotyledons in two burnt and unburnt areas of Sirachal Station. Based on the statistical analysis, using Past3 and SPSS softwares, there was no significant difference in the number of taxa between the vegetation of the burnt and unburnt areas. Of the indicators analyzed in Past3, the number of individual, the index of Menhinick's richness and the Shannon diversity index were significantly different in the treatments sampled from the burnt and unburnt areas. According to the Duncan test average, the number of individuals in the unburnt area, with a canopy cover below 25%, was greater than that in the burnt area, with the same canopy cover, however, no significant difference was observed between the individuals in the burnt area and unburnt area, with the same canopy cover of more than 25%. Also, the richness of the burnt area with a canopy of less than 5% is significantly higher than that in the unburnt area with the same canopy cover.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46195630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
. In order to investigate the differences in the shape of the big-head and two morphotypes of Phytophagous fish by geometric morphometric technique, 30 samples of each group, with a mean standard length of 25 ± 3 cm, were prepared. After fish were anesthetized by 1% Clove powder extract and stabilized in formalin buffer 4%, 6-megapixel digital images of the left profile of the specimens were prepared. A total of 17 morphological landmarks were digitized by tpsDig2 software and the tps files were prepared. All non-shaped data were removed from landmark coordinates by generalized procrustes analysis. After the approval of regression between shape space and tangent space, data were analyzed using Principal Component (PCA), Canonical Variation (CVA) and Cluster Analyzes (CA) methods in PAST and TPS series. According to the results, the head length and height, the size of dorsal fin, the length of pre ventral distance, the height of caudal peduncle and the degree of posterior position of the pectoral fin in bighead carp, are some of the distinctive morphological features among species, mainly related to their ecological niche (specially feeding strategies). In the case of silver carp variants, the posterior position of pectoral fin base in silver carp-R that is close to bighead carp, may be due to hybridization between bighead carp and silver carps.
{"title":"Body shape comparison of Big-head carp with two variants of silver carp using geometric morphometric techniques","authors":"M. Nasri, F. Ghojoghi","doi":"10.29252/NBR.6.4.382","DOIUrl":"https://doi.org/10.29252/NBR.6.4.382","url":null,"abstract":". In order to investigate the differences in the shape of the big-head and two morphotypes of Phytophagous fish by geometric morphometric technique, 30 samples of each group, with a mean standard length of 25 ± 3 cm, were prepared. After fish were anesthetized by 1% Clove powder extract and stabilized in formalin buffer 4%, 6-megapixel digital images of the left profile of the specimens were prepared. A total of 17 morphological landmarks were digitized by tpsDig2 software and the tps files were prepared. All non-shaped data were removed from landmark coordinates by generalized procrustes analysis. After the approval of regression between shape space and tangent space, data were analyzed using Principal Component (PCA), Canonical Variation (CVA) and Cluster Analyzes (CA) methods in PAST and TPS series. According to the results, the head length and height, the size of dorsal fin, the length of pre ventral distance, the height of caudal peduncle and the degree of posterior position of the pectoral fin in bighead carp, are some of the distinctive morphological features among species, mainly related to their ecological niche (specially feeding strategies). In the case of silver carp variants, the posterior position of pectoral fin base in silver carp-R that is close to bighead carp, may be due to hybridization between bighead carp and silver carps.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45800191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the most important factors related to male fertility is sperm chromatin status. Under Assisted Reproductive Techniques (ART), especially Intracytoplasmic Sperm Injection (ICSI), the natural selection of high quality sperm with intact chromatin is not possible. Therefore, embryo quality and pregnancy rate decreased for damaged DNA of sperms involved in the fertilization of eggs. Thus, this study evaluated the frequency of abnormal sperm chromatin condensation and damaged sperm chromatin in men with different infertility factors (e.g. oligozoospermia, asthenozoospermia, teratozoospermia, oligasthenoteratozoospermia, and normozoospermia) and ICSI outcomes were examined. 195 patients were examined and the sperm chromatin status was evaluated using aniline blue and toluidine blue staining. Fertilization, zygote and embryo quality, chemical pregnancy and abortion rates were calculated, too. The results showed that in the oligoasthenoteratozzoospermia samples, the damaged chromatin percentage was higher than that in normozoospermia ones. The fertilization rate, embryo quality, and pregnancy rate significantly decreased in this group. Also, a higher abortion rate was observed in this group (P<0.05). In conclusion, the frequency of damaged chromatin was observed to be different in different male infertility factors, which could influence the ICSI outcomes. Therefore, the evaluation of sperm chromatin status before cycle initiation was found to promote ICSI outcomes.
{"title":"The evaluation of sperm chromatin status in men with different infertility factors, and its relationship with ICSI outcomes","authors":"F. Ghasemian","doi":"10.29252/NBR.6.4.367","DOIUrl":"https://doi.org/10.29252/NBR.6.4.367","url":null,"abstract":"One of the most important factors related to male fertility is sperm chromatin status. Under Assisted Reproductive Techniques (ART), especially Intracytoplasmic Sperm Injection (ICSI), the natural selection of high quality sperm with intact chromatin is not possible. Therefore, embryo quality and pregnancy rate decreased for damaged DNA of sperms involved in the fertilization of eggs. Thus, this study evaluated the frequency of abnormal sperm chromatin condensation and damaged sperm chromatin in men with different infertility factors (e.g. oligozoospermia, asthenozoospermia, teratozoospermia, oligasthenoteratozoospermia, and normozoospermia) and ICSI outcomes were examined. 195 patients were examined and the sperm chromatin status was evaluated using aniline blue and toluidine blue staining. Fertilization, zygote and embryo quality, chemical pregnancy and abortion rates were calculated, too. The results showed that in the oligoasthenoteratozzoospermia samples, the damaged chromatin percentage was higher than that in normozoospermia ones. The fertilization rate, embryo quality, and pregnancy rate significantly decreased in this group. Also, a higher abortion rate was observed in this group (P<0.05). In conclusion, the frequency of damaged chromatin was observed to be different in different male infertility factors, which could influence the ICSI outcomes. Therefore, the evaluation of sperm chromatin status before cycle initiation was found to promote ICSI outcomes.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42100771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Daneshvar, M. Maleki, S. Shakeri, A. Baghizadeh
. Phosphorus, the most essential nutrient for plants, becomes quickly unavailable for the plants in the soil. Phosphate solubilizing bacteria (PSB( can play an important role in providing Phosphorus for plants. In this study, the PSBs were screened from plant rhizosphere by Pikovskaya method. Then, the growth rate and phosphate solubilizing ability of 9 superior strains were measured at different temperatures and levels of salinity and pH. The best strain was identified by 16S rDNA gene sequence analysis. Finally, the genetic diversity of phosphate solubilizing strains were examined by RAPD markers. Results showed that 25 strains were capable of solubilizing insoluble phosphates among the 57 isolates studied. Of the nine superior strains, Cke1 had the highest solubilizing index with the average growth rate under all conditions and was introduced as the best PSB strain identified in the present study. 16S rDNA gene sequence analysis showed that this strain belonged to the Enterobacter genus. The results of genetic variation showed that all stains
{"title":"Screening and identification of Iranian native phosphate solubilizing bacteria and investigation of their genetic diversity using RAPD markers","authors":"M. Daneshvar, M. Maleki, S. Shakeri, A. Baghizadeh","doi":"10.29252/NBR.6.4.402","DOIUrl":"https://doi.org/10.29252/NBR.6.4.402","url":null,"abstract":". Phosphorus, the most essential nutrient for plants, becomes quickly unavailable for the plants in the soil. Phosphate solubilizing bacteria (PSB( can play an important role in providing Phosphorus for plants. In this study, the PSBs were screened from plant rhizosphere by Pikovskaya method. Then, the growth rate and phosphate solubilizing ability of 9 superior strains were measured at different temperatures and levels of salinity and pH. The best strain was identified by 16S rDNA gene sequence analysis. Finally, the genetic diversity of phosphate solubilizing strains were examined by RAPD markers. Results showed that 25 strains were capable of solubilizing insoluble phosphates among the 57 isolates studied. Of the nine superior strains, Cke1 had the highest solubilizing index with the average growth rate under all conditions and was introduced as the best PSB strain identified in the present study. 16S rDNA gene sequence analysis showed that this strain belonged to the Enterobacter genus. The results of genetic variation showed that all stains","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45182544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
. The cement production activities leads to the release of different pollutants into the environment. This research was conducted to study the effects of dust particles released by Behbahan cement factory on the absorption of some nutrient elements and biochemical parameters in dominant herbaceous species grown in this area, including Bromus tectorum, Malva neglecta and Sinapis arvensis . Plant samples were transferred to the laboratory. Then, the concentrations of some nutrient elements, such as Fe, Cu, Zn, K, Mn, P and N, were assayed in plant and soil samples. Moreover, some biochemical parameters, such as photosynthetic pigments, total protein, soluble carbohydrates, proline, relative water content, leaf pH total ascorbate and air pollution tolerance indexes (APTI), were evaluated. Based on these results, it is supposed that three plant species are tolerant to air pollution with an APTI higher than 16. In addition, it seems that the dust released by the cement factory has not led to a toxic accumulation of the elements in the studied plants. The deficiency of Mn and P was determined for three plant species. On the basis of biochemical analysis, it is supposed that these plant species possibly use different strategies to tolerate the pollutants in this area.
{"title":"The effects of Behbahan cement factory activity on the absorption of some nutrients and biochemical responses in herbaceous plants Sinapis arvensis, Malva neglecta and Bromus tectorum","authors":"Maryam Masoudizadeh, P. Zoufan, S. Rastegarzadeh","doi":"10.29252/NBR.6.4.464","DOIUrl":"https://doi.org/10.29252/NBR.6.4.464","url":null,"abstract":". The cement production activities leads to the release of different pollutants into the environment. This research was conducted to study the effects of dust particles released by Behbahan cement factory on the absorption of some nutrient elements and biochemical parameters in dominant herbaceous species grown in this area, including Bromus tectorum, Malva neglecta and Sinapis arvensis . Plant samples were transferred to the laboratory. Then, the concentrations of some nutrient elements, such as Fe, Cu, Zn, K, Mn, P and N, were assayed in plant and soil samples. Moreover, some biochemical parameters, such as photosynthetic pigments, total protein, soluble carbohydrates, proline, relative water content, leaf pH total ascorbate and air pollution tolerance indexes (APTI), were evaluated. Based on these results, it is supposed that three plant species are tolerant to air pollution with an APTI higher than 16. In addition, it seems that the dust released by the cement factory has not led to a toxic accumulation of the elements in the studied plants. The deficiency of Mn and P was determined for three plant species. On the basis of biochemical analysis, it is supposed that these plant species possibly use different strategies to tolerate the pollutants in this area.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45237534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elaheh Zadeh-Hosseingholi, N. Chaparzadeh, Samira Mahmudi Aghdam
Some rhizobacteria have positive effects on plants growth. Syrian bean-caper (Zygophyllum fabago) is a weed plant with medicinal value. This study was conducted to isolate and identify bacteria from Syrian bean-caper rhizosphere. Characteristics associated with plant growth stimulation, such as phosphate and zinc dissolution, production of Indole acetic acid and antifungal activity, were investigated. The isolates were separately inoculated to the plant and after plant root establishment was ensured, their effectiveness in increasing plant growth in greenhouse conditions was measured. Biochemical and molecular identification results showed that five isolates belonged to the genera Bacillus, Pseudomonas, Pantoea, and Brevibacterium. All five isolates showed some degree of plant growth promotion capabilities. Among the isolates, only the genus Bacillus increased the dry weights of plants significantly. The amount of phosphate solubilization for this isolate was 440 μg ml and its acid production in the culture medium was higher than that in other isolates. The isolate had zinc solubilisation capability and produced 3.89 mg ml indole acetic acid. However, this isolate did not show antifungal activity against two fungal pathogens of Aspergillus niger and Botrytis cinerea.
{"title":"Isolation and characterization of plant growth-promoting bacteria from Syrian bean caper (Zygophyllum fabago) rhizosphere","authors":"Elaheh Zadeh-Hosseingholi, N. Chaparzadeh, Samira Mahmudi Aghdam","doi":"10.29252/NBR.6.4.435","DOIUrl":"https://doi.org/10.29252/NBR.6.4.435","url":null,"abstract":"Some rhizobacteria have positive effects on plants growth. Syrian bean-caper (Zygophyllum fabago) is a weed plant with medicinal value. This study was conducted to isolate and identify bacteria from Syrian bean-caper rhizosphere. Characteristics associated with plant growth stimulation, such as phosphate and zinc dissolution, production of Indole acetic acid and antifungal activity, were investigated. The isolates were separately inoculated to the plant and after plant root establishment was ensured, their effectiveness in increasing plant growth in greenhouse conditions was measured. Biochemical and molecular identification results showed that five isolates belonged to the genera Bacillus, Pseudomonas, Pantoea, and Brevibacterium. All five isolates showed some degree of plant growth promotion capabilities. Among the isolates, only the genus Bacillus increased the dry weights of plants significantly. The amount of phosphate solubilization for this isolate was 440 μg ml and its acid production in the culture medium was higher than that in other isolates. The isolate had zinc solubilisation capability and produced 3.89 mg ml indole acetic acid. However, this isolate did not show antifungal activity against two fungal pathogens of Aspergillus niger and Botrytis cinerea.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43462371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Khosroshahi, E. Salehghamari, M. A. Amoozegar, P. Saffarian
Nowadays plant endophytic bacteria have found diverse and useful applications in biotechnology; therefore, much attention has been paid to the isolation, identification, and evaluation of these microorganisms. Since the sterilizing plant tissue surfaces from epiphytic bacteria is difficulty, the efficacy of three different screening methods for endophytic bacteria including 1HClO sterilization, 2Periodic sterilization (modified tyndallization) and 3Triton X100 and HClO sterilization, was evaluated in this study. The modified Tyndallization is an innovative method used in this study to appropriately remove the internal spores of epiphytic bacteria, considered to be an obstacle to the isolation of endophytes. Most of the endophytic bacteria were isolated from dicotyledons and leaves. Endophytic bacteria were also studied for the production of different hydrolase enzymes, whereas the protease enzyme was produced in a wide range of endophytic bacteria in greater quantities than other enzymes. The EndoA strain was molecularly identified and found to be 100% similar to Bacillus halotolerans.
{"title":"Screening methods and enzyme profile of agricultural plant-endophytic bacteria","authors":"S. Khosroshahi, E. Salehghamari, M. A. Amoozegar, P. Saffarian","doi":"10.29252/NBR.6.4.415","DOIUrl":"https://doi.org/10.29252/NBR.6.4.415","url":null,"abstract":"Nowadays plant endophytic bacteria have found diverse and useful applications in biotechnology; therefore, much attention has been paid to the isolation, identification, and evaluation of these microorganisms. Since the sterilizing plant tissue surfaces from epiphytic bacteria is difficulty, the efficacy of three different screening methods for endophytic bacteria including 1HClO sterilization, 2Periodic sterilization (modified tyndallization) and 3Triton X100 and HClO sterilization, was evaluated in this study. The modified Tyndallization is an innovative method used in this study to appropriately remove the internal spores of epiphytic bacteria, considered to be an obstacle to the isolation of endophytes. Most of the endophytic bacteria were isolated from dicotyledons and leaves. Endophytic bacteria were also studied for the production of different hydrolase enzymes, whereas the protease enzyme was produced in a wide range of endophytic bacteria in greater quantities than other enzymes. The EndoA strain was molecularly identified and found to be 100% similar to Bacillus halotolerans.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49283996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：