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Erratum to: Comparative Pharmacoeconomic Effectiveness of Interleukin-17 Inhibitors for the Treatment of Ankylosing Spondylitis 勘误:白细胞介素-17抑制剂治疗强直性脊柱炎的药物经济学效果比较。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-24 DOI: 10.1134/S1607672923050058
T. V. Dubinina, I. Z. Gaidukova, N. A. Sableva, K. V. Sapozhnikov, V. D. Sokolova, D. G. Tolkacheva
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引用次数: 0
Erratum to: Anti-topoisomerase 1 Antibody Level Changes after B Cell Depletion Therapy in Systemic Sclerosis 勘误:系统性硬化症患者接受 B 细胞清除疗法后抗拓扑异构酶 1 抗体水平的变化。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-24 DOI: 10.1134/S1607672923050022
L. P. Ananyeva, L. A. Garzanova, O. A. Koneva, M. N. Starovoytova, O. V. Desinova, O. B. Ovsyannikova, R. U. Shayakhmetova, M. V. Cherkasova, A. P. Aleksankin, E. L. Nasonov
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引用次数: 0
Erratum to: Contributing Factors of Diabetes Mellitus among Patients with Gout (Results of the Long-Term Prospective Study) 勘误:痛风患者患糖尿病的诱因(长期前瞻性研究结果)。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-24 DOI: 10.1134/S1607672923050083
O. V. Zheliabina, M. S. Eliseev, S. I. Glukhova, E. L. Nasonov
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引用次数: 0
Erratum to: Global Antiphospholipid Syndrome Score (GAPSS) in Patients with Systemic Lupus Erythematosus 勘误:系统性红斑狼疮患者的全球抗磷脂综合征评分 (GAPSS)。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-24 DOI: 10.1134/S1607672923050046
F. A. Cheldieva, T. M. Reshetnyak, A. A. Shumilova, K. S. Nurbaeva, M. V. Cherkasova, A. M. Lila, E. L. Nasonov
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引用次数: 0
HspBP1 in Complex with the Peptide of the Innate Immunity Protein Tag7 is Able to Lyse Tumor Cells Carrying TNFR1 Receptor HspBP1 与先天性免疫蛋白 Tag7 的多肽复合物能溶解携带 TNFR1 受体的肿瘤细胞。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-08 DOI: 10.1134/S1607672923700631
E. A. Romanova, D. M. Yurkina, D. V. Yashin, L. P. Sashchenko,  G. P. Georgiev

The search for new cytotoxic agents capable of lysing tumor cells is an important task in the fight against cancer. Here we have shown that the HspBP1 protein, the chaperone of the heat shock protein Hsp70, is able to form a complex with the previously discovered peptide (17.1) of the innate immunity protein Tag7. Experiments using thermophoresis demonstrated that the affinity of the Tag7 protein peptide 17.1 to the HspBP1 molecule is 100 times higher than that of the full-sized Tag7 molecule. The addition of the 17.1–HspBP1 complex to tumor cells induces apoptosis and necroptosis in them. The results obtained in this work can be used to develop promising antitumor drugs.

寻找能够溶解肿瘤细胞的新型细胞毒剂是抗击癌症的一项重要任务。在这里,我们发现热休克蛋白 Hsp70 的伴侣蛋白 HspBP1 能够与之前发现的先天免疫蛋白 Tag7 的多肽(17.1)形成复合物。利用热电泳技术进行的实验表明,Tag7 蛋白多肽 17.1 与 HspBP1 分子的亲和力比完整尺寸的 Tag7 分子高 100 倍。在肿瘤细胞中加入 17.1-HspBP1 复合物可诱导细胞凋亡和坏死。这项研究的结果可用于开发有前景的抗肿瘤药物。
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引用次数: 0
Melanophilin Polymorphism in Ferrets of Different Color 不同肤色雪貂的嗜黑素多态性
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-08 DOI: 10.1134/S1607672923700655
G. Yu. Kosovsky, V. I. Glazko, O. I. Abramov, T. T. Glazko

In mammals, the main contribution to the variability of pigmentation is made by two groups of genes directly related to the metabolic pathways of pigment synthesis and controlling the transport of melanosomes in melanocytes to keratinocytes. In order to identify the genetic basis of pigmentation variants, the nucleotide sequences of the melanophilin gene were compared in two groups of ferrets—silver-colored and wild-type animals—using sequencing of 16 exons. In carriers of silver color, a single nucleotide deletion was detected in the 9th exon, leading to a shift in the reading frame and the formation of a stop codon downstream. The protein encoded by the mutant allele is almost completely devoid of the C terminal domain of the protein responsible for the contact of melanosomes with actin during their moving to the periphery of melanocytes, but it retains the leading domain involved in the formation of melanosomes. The combination of the preservation of the N domain and the defect of the C domain of the mutant protein for the first time makes it possible to explain the incomplete dominance of the wild-type protein in heterozygotes.

在哺乳动物中,色素变异的主要原因是与色素合成代谢途径直接相关的两组基因,它们控制着黑色素细胞中的黑色素小体向角质细胞的运输。为了确定色素变异的遗传基础,研究人员通过对 16 个外显子进行测序,比较了两组雪貂--银色雪貂和野生型雪貂--嗜黑素蛋白基因的核苷酸序列。在银色携带者中,第 9 个外显子中发现了一个单核苷酸缺失,导致阅读框发生偏移,并在下游形成了一个终止密码子。突变等位基因编码的蛋白质几乎完全没有C末端结构域,该结构域负责黑色素小体向黑色素细胞外周移动过程中与肌动蛋白的接触,但保留了参与黑色素小体形成的前导结构域。突变体蛋白质 N 结构域的保留和 C 结构域的缺陷首次结合在一起,这就有可能解释野生型蛋白质在杂合子中的不完全优势。
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引用次数: 0
Neuronal and Muscle Differentiation of Mammalian Cells Is Accompanied by a Change in PHF10 Isoform Expression 哺乳动物细胞的神经元和肌肉分化伴随着 PHF10 同工酶表达的变化
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-08 DOI: 10.1134/S1607672923700643
D. O. Bayramova, A. M. Azieva, A. V. Feoktistov,  S. G. Georgieva, N. V. Soshnikova

The PBAF chromatin remodeling complex of the SWI/SNF family plays a critical role in the regulation of gene expression during tissue differentiation and organism development. The subunits of the PBAF complex have domains responsible for binding to N-terminal histone sequences. It determines the specificity of binding of the complex to chromatin. PHF10, a specific subunit of the PBAF complex, contains a DPF domain, which is a unique chromatin interaction domain. A PHF10 isoform that lacks the DPF domain is also present in vertebrate cells. This work shows that during neuronal and muscle differentiation of human and mouse cells, the expression of PHF10 isoforms changes: the form that does not have DPF replaces the form in which it is present. Replacement of PHF10 isoforms in the PBAF complex may affect its selectivity in the regulation of genes in differentiating cells.

SWI/SNF 家族的 PBAF 染色质重塑复合体在组织分化和生物体发育过程中的基因表达调控中发挥着关键作用。PBAF 复合物的亚基具有负责与 N 端组蛋白序列结合的结构域。它决定了复合体与染色质结合的特异性。PHF10 是 PBAF 复合物的一个特定亚基,含有 DPF 结构域,这是一个独特的染色质相互作用结构域。脊椎动物细胞中也存在缺乏 DPF 结构域的 PHF10 异构体。这项研究表明,在人类和小鼠细胞的神经元和肌肉分化过程中,PHF10 同工型的表达发生了变化:没有 DPF 的同工型取代了有 DPF 的同工型。PBAF复合体中PHF10异构体的替代可能会影响其在分化细胞中调控基因的选择性。
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引用次数: 0
Dichotomic Role of Low-Concentration EGCG in the Oxaliplatin Sensitivity of Colorectal Cancer Cells 低浓度 EGCG 对大肠癌细胞奥沙利铂敏感性的二分作用
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-08 DOI: 10.1134/S160767292360029X
Zhiyong Wang, Min Wang, Jiahao Huang, Mao Lin, Pei Wei

Although epigallocatechin-3-gallate (EGCG) can potentiate chemotherapeutic drugs at high concentrations, its clinical translation is hampered by exceeding possible concentration thresholds. This study proposes a dichotomous use of low-concentration EGCG in chemotherapy. During the first cycle of combined treatment with oxaliplatin (OXA), low-concentration EGCG antagonized the cytotoxic effect of OXA on colorectal cancer (CRC) cells. However, when OXA was subsequently administered, the sensitivity of CRC cells markedly increased. Although low-concentration EGCG counteracted OXA, it reduced the OXA-induced secretion of vascular endothelial growth factor by tumor cells, thereby contributing to the increase in the sensitivity of tumor cells to the second round of OXA treatment. Therefore, low-concentration EGCG showed potential as a viable adjunct to modulate chemosensitivity in CRC.

尽管表没食子儿茶素-3-棓酸盐(EGCG)在高浓度下可增强化疗药物的疗效,但其临床应用却因超过可能的浓度阈值而受到阻碍。本研究提出了在化疗中使用低浓度 EGCG 的两分法。在与奥沙利铂(OXA)联合治疗的第一周期中,低浓度 EGCG 可拮抗 OXA 对结直肠癌细胞的细胞毒性作用。然而,当随后服用 OXA 时,CRC 细胞的敏感性明显增加。虽然低浓度的EGCG能抵消OXA的作用,但它能减少肿瘤细胞分泌由OXA诱导的血管内皮生长因子,从而导致肿瘤细胞对第二轮OXA治疗的敏感性增加。因此,低浓度EGCG有望成为调节CRC化疗敏感性的有效辅助药物。
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引用次数: 0
N6-(5-Phenylpentan-1-yl)adenine—A New Non-competitive Receptor-Specific Anti-cytokinin N6-(5-苯基戊-1-基)腺嘌呤--一种新的非竞争性受体特异性抗细胞分裂素。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-08 DOI: 10.1134/S1607672923700679
A. A. Zenchenko, E. M. Savelieva, M. S. Drenichev, G. A. Romanov, V. E. Oslovsky

For the first time, N6-(5-phenylpentan-1-yl)adenine, a synthetic adenine derivative with a receptor-specific anticytokinin effect, was obtained. This compound exhibits a pronounced anticytokinin effect, reducing cytokinin-induced expression of the GUS reporter gene when interacting with the cytokinin receptor CRE1/AHK4 of the model plant Arabidopsis thaliana. This effect manifests itself much weaker with the related AHK2 receptor and is not observed at all with the AHK3 receptor. We showed that N6-(5-phenylpentan-1-yl)adenine does not bind to the ligand-binding sites of the Arabidopsis cytokinin receptors, which does not allow it to be classified as a true cytokinin antagonist. Despite the currently unknown mechanism of action, this compound may find its use as a component of plant growth regulators. Like true anticytokinins, it enhances root growth of Arabidopsis seedlings, apparently suppressing the action of endogenous cytokinins on the “root” receptor CRE1/AHK4.

首次获得了具有受体特异性抗细胞分裂素效应的合成腺嘌呤衍生物 N6-(5-苯基戊-1-基)腺嘌呤。这种化合物具有明显的抗细胞分裂素效应,在与模式植物拟南芥的细胞分裂素受体 CRE1/AHK4 相互作用时,能减少细胞分裂素诱导的 GUS 报告基因的表达。这种作用在与之相关的 AHK2 受体上表现得更弱,而在 AHK3 受体上则完全观察不到。我们的研究表明,N6-(5-苯基戊-1-基)腺嘌呤不能与拟南芥细胞分裂素受体的配体结合位点结合,因此不能将其归类为真正的细胞分裂素拮抗剂。尽管这种化合物的作用机理目前尚不清楚,但它可能被用作植物生长调节剂的一种成分。与真正的抗细胞分裂素一样,它能促进拟南芥幼苗根部的生长,显然能抑制内源细胞分裂素对 "根 "受体 CRE1/AHK4 的作用。
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引用次数: 0
Contact Inhibition of Proliferation Is Accompanied by Expression of the PHF10D Subunit of the Chromatin Remodeling Complex PBAF in Mouse and Human Cell Lines 在小鼠和人类细胞系中,染色质重塑复合物 PBAF 的 PHF10D 亚基的表达伴随着增殖的接触抑制。
IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-08 DOI: 10.1134/S1607672923700667
Yu. P. Simonov, V. V. Tatarskiy,  S. G. Georgieva, N. V. Soshnikova

PHF10 is a subunit of the PBAF complex, which regulates the expression of many genes in developing and maturing organisms. PHF10 has four isoforms that differ in domain structure. The PHF10A isoform, containing a DPF domain at the C-terminus and 46 amino acids at the N-terminus, is necessary for the expression of proliferation genes; the functions of the other isoforms are less studied. In this work, we have established that, upon contact inhibition of mouse and human cell proliferation caused by the establishment of a tight junction and adherence junction between cells, the expression of the PHF10A isoform stops and instead the PHF10D isoform is expressed, which does not contain DPF-domain and N-terminal sequence. The function of the PHF10D isoform may be associated with the establishment of intercellular contacts.

PHF10 是 PBAF 复合物的一个亚基,PBAF 复合物调节发育和成熟生物体中许多基因的表达。PHF10 有四种异构体,它们的结构域各不相同。PHF10A 异构体的 C 端含有 DPF 结构域,N 端含有 46 个氨基酸,是增殖基因表达所必需的;其他异构体的功能研究较少。在这项工作中,我们证实了当细胞之间建立紧密连接和粘附连接而导致小鼠和人类细胞增殖受到接触性抑制时,PHF10A 同工酶的表达会停止,取而代之的是不含 DPF 结构域和 N 端序列的 PHF10D 同工酶的表达。PHF10D 异构体的功能可能与细胞间接触的建立有关。
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引用次数: 0
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Doklady Biochemistry and Biophysics
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