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Aktivitas antioksidan dan antibakteri dari ekstrak bee pollen lebah kelulut (Tetragonula sarawaknensis) 蜂毒提取物中的抗氧化剂和抗菌活性。
Pub Date : 2021-12-31 DOI: 10.24111/jrihh.v13i2.7050
Ayu Mitha Sari, Enih Rosamah, Wiwin Suwinarti, I. W. Kusuma, Enos Tangke Arung, Ph.D.
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引用次数: 1
Optimasi pirolisis limbah kayu Durian (Durio zibethinus) berdasarkan ukuran partikel dan suhu 榴莲木材废水优化,根据颗粒大小和温度
Pub Date : 2021-12-31 DOI: 10.24111/jrihh.v13i2.7034
Dr. Evy Setiawati, M.T., Wahida Anisa Yusuf, Heri Soedarmanto
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引用次数: 1
In-vitro antioxidant and antibacterial activities of tegeran wood (Cudrania javanensisTrécul) extracts [Aktivitas antioksidan dan antibakteri ekstrak kayu tegeran secara in-vitro(Cudrania javanensisTrécul)]
Pub Date : 2020-12-31 DOI: 10.24111/jrihh.v12i2.6476
C. Darsih, A. W. Indrianingsih, A. Windarsih, Khoirun Nisa, V. T. Rosyida, S. Handayani, M. P. Wulanjati, N Indirayati, D. Ratih
Cudrania javanensis generally used as natural dyes in Indonesia, but limited is known about its biological activities. The study aimed to assess in vitro antioxidant and antibacterial activities of C. javanensis crude extracts. The antioxidant properties of crude extracts were determined by the DPPH free radical and ABTS method. M ethanol and water extracts were also evaluated for their antimicrobial activities toward strain of Gram positive bacteria ( Staphylococcus aureus ) and Gram negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ) by the agar well diffusion method. Th e results indicated that C. javanensis wood methanol extract exhibited good antioxidant activity than water extract , which against DPPH radical with IC 50 of 12.23±1.43 μg/mL, and scavenging to ABTS radical about 964.69±15.05 mg trolox equivalent/g at 0.02 5 mg/mL, respectively. However, both of extracts did not possess activity toward antibacterial assay. This study indicated that methanol and water extracts from C. javanensis wood could be used as natural antioxidant resources.
javanensis在印度尼西亚一般作为天然染料使用,但对其生物活性的了解有限。本研究旨在评价爪哇木粗提物的体外抗氧化和抗菌活性。采用DPPH自由基法和ABTS法测定粗提物的抗氧化性能。用琼脂孔扩散法测定了乙醇提取物和水提取物对革兰氏阳性菌(金黄色葡萄球菌)和革兰氏阴性菌(大肠杆菌和铜绿假单胞菌)的抑菌活性。结果表明,爪牙木甲醇提取物对DPPH自由基的ic50为12.23±1.43 μg/mL,对ABTS自由基的ic50为964.69±15.05 mg trolox当量/g,浓度为0.02 5 mg/mL。但两种提取物均不具有抗菌活性。该研究表明,爪哇木木材的甲醇和水提取物可作为天然抗氧化资源。
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引用次数: 0
Potensi ekstrak buah ramania (Bouea macrophylla Griffith) sebagai antibakteri terhadap beberapa bakteri patogen [The potential of ramania fruit extract (Bouea macrophylla Griffith) as an antibacterial against several pathogenic bacteria]
Pub Date : 2020-12-31 DOI: 10.24111/jrihh.v12i2.6277
S.Si. Nadra Khairiah, Rais Salim
Ramania fruit ( Bouea macrophylla Griffith) is a tropical plants, as a new natural source of polyphenols. The content of these polyphenols is very abundant, especially in the seeds. The polyphenol content is widely used in the pharmaceutical world, as antibacterial ingredient. The development of natural antibacterial compounds from plants is needed to be used as a therapeutic treatment for bacterial infectious diseases. The purpose of this study is to determine the potential of ramania seed extract, seed coat and pulp against several types of pathogenic bacteria ( Escherichia coli, Enterococcus aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella thypimurium, and Propionibacterium acnes ) that generally cause disease in humans. Ramania fruit is divided into three parts, such asseeds, seed coatand pulp. Antibacterial testing was done by agar diffusion method. The concentration of the extract for antibacterial tests were 1000 ppm, 500 ppm and 250 ppm.The MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values were calculated using the microdilution method using 96-well micro-titre plates. The results showed that ramania seed extract had the highest antibacterial activity compared to seed coat extract and pulp. Inhibitory zone of Ramania seed extract ranged from 23-30 mm at a concentration of 1000 ppm and still had inhibition effect up to concentration of 250 ppm. MIC value of seed extract with methanol solvent was 78.13 μg/mL against three types of bacteria ( P. aeruginosa, P. acnes, and S. thypimurium ).
拉曼尼亚果(Bouea macrophylla Griffith)是一种热带植物,是一种新的天然多酚来源。这些多酚的含量非常丰富,特别是在种子中。其多酚含量广泛应用于医药领域,作为抗菌成分。从植物中开发天然抗菌化合物是治疗细菌性感染性疾病的必要手段。本研究的目的是确定拉曼尼亚种子提取物、种皮和果肉对几种致病菌(大肠杆菌、产气肠球菌、金黄色葡萄球菌、铜绿假单胞菌、胸腺菌沙门氏菌和痤疮丙酸杆菌)的潜在抗性,这些致病菌通常会引起人类疾病。拉马尼亚果实分为种子、种皮和果肉三部分。采用琼脂扩散法进行抑菌试验。抗菌试验提取液的浓度分别为1000 ppm、500 ppm和250 ppm。采用96孔微滴板微稀释法计算最小抑菌浓度(MIC)和最小杀菌浓度(MBC)值。结果表明,拉曼尼亚籽提取物的抑菌活性高于种皮提取物和果肉提取物。在1000 ppm浓度下,拉曼尼亚种子提取物的抑制带在23 ~ 30 mm之间,在250 ppm浓度下仍有抑制作用。甲醇溶剂对铜绿假单胞菌(P. aeruginosa)、痤疮假单胞菌(P. acnes)和胸腺脓毒杆菌(S. thypimurium) 3种细菌的MIC值为78.13 μg/mL。
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引用次数: 0
Pengaruh Pemberian Asap Cair Galam pada Edibel Film Terhadap Kemunduran Mutu Ikan Patin (Pangasius sp.) [The effect of galam liquid smoke (Malaleuca leucadendra) incorporated with edible film as preservative for catfish (Pangasius sp.) during storage]
Pub Date : 2020-12-31 DOI: 10.24111/jrihh.v12i2.6269
Rais Salim, Nadra Khairiah, N. Rahmi
Galam l iquid smoke is a liquid product derived from c arbonization process of Galam wood (Malaleuca leucadendra) which can be used as natural preservative to fish. The incorporated edible film packaging with l iquid galam wood is a good alternative to inhibit the deterioration of fish qua lity during storage. This study aims to determine the effect of liquid smoke of galam wood in corporated into edibl e film to the deterioration of catfish (Pangasius sp) quality. The treatment factors used were (i) the edible film - gelatin e formulation (G), g elatin e - pectin (G - P), liquid smoke - gel atine (G - AC) and gelatine - pectin - liquid smoke (G - P - AC). It was compared with control (without treatment). The other factors were (ii) storage duration of 0 hour, 2 hours, 4 hours and 6 hours. The observed degradation p arameters were Total Volatile Base - Nitrogen (TVBN) and microbiological testing. After being stored for 6 hours , t he highest value of TVBN was found in the control without treatment ( 54 mgN/100g ), while the lowest TVBN value was g elatine - pectine - liquid smok e (1 8 mgN/100g ) . T he microbiological test showed that the highest TP value was found in the control which reached 1 . 6x10^9 , while the gelatine, gelatine - pectin, gelatine-l iquid smoke and gelatine - pectin - liquid smoke were log 5 . 7x10^8 ; 1 . 0x10^8 ; 3 . 5x10^8 ; and 7 . 0x10^8 respectively. The gelatin e - pectin - liquid smoke (G - P - AC) treatment is able to inhibit the decrease of catfish quality on TVBN value at 6 hours of storage, while TPC value is more stable on gelatine - smoke treatment (G - AC) at 2 to 6 hours. The results of this study indicate that the addition of liquid smoke is able to inhibit the declining quality of catfish.
甘露木液体烟是甘露木(Malaleuca leucadendra)经炭化加工而成的液体产品,可作为鱼类的天然防腐剂。混合食用膜包装的液体甘莲木是抑制鱼在储存过程中品质劣化的一种很好的选择。本研究旨在探讨食用膜中掺入香木液烟对鲶鱼(Pangasius sp)品质恶化的影响。采用的处理因素为(1)食用薄膜-明胶e配方(G)、明胶-果胶(G - P)、液烟-凝胶丁(G - AC)和明胶-果胶-液烟(G - P - AC)。与对照组(未经治疗)进行比较。其他影响因素为(ii)贮藏时间0小时、2小时、4小时和6小时。观察到的降解参数有总挥发性碱氮(TVBN)和微生物学测试。贮藏6 h后,未处理对照的TVBN值最高(54 mgN/100g),最低的TVBN值为果胶-液烟e (18 mgN/100g)。微生物学试验结果表明,对照组TP值最高,达1。而明胶、明胶-果胶、明胶-液体烟和明胶-果胶-液体烟均为log 5。7 8 x10 ^;1 . 0 8 x10 ^;3.。5 8 x10 ^;和7。分别为0 8 x10 ^。明胶-果胶-液烟(G - P - AC)处理能抑制鲶鱼品质在贮藏6 h时TVBN值的下降,而明胶-液烟(G - AC)处理在贮藏2 ~ 6 h时TPC值较为稳定。本研究结果表明,液体烟的加入能够抑制鲶鱼品质的下降。
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引用次数: 1
Performance comparison of liquid smokes from galam wood, rubber wood, and oil palm frond as latex coagulants on the quality aspects of the rubber cup lump 以甘兰木、橡胶木、油棕叶为胶乳混凝剂的液体烟气对橡胶杯块质量的影响比较
Pub Date : 2019-10-30 DOI: 10.24111/JRIHH.V11I1.4619
Agung Nugroho, Ph.D., Iqbal Maulana Majid, Hisyam Musthafa Al Hakim
The quality of rubber cup lump is ditermined by several factors, one of them is the latex coagulant. The type of latex coagulant also affects the coagulating time. Liquid smoke is one of the most recommended latex coagulants due its save and effective operation. Deorub, the most popular liquid smoke, is made from shell of oil palm kernel. There are still many alternatives of liquid smoke materials that may have lower economic values. This study was aimed to evaluate and compare the performance of three liquid smokes produced from three alternative materials namely galam wood, rubber wood, and oil palm frond as latex coagulants. This study was performed using groups random design with two factors, namely type of coagulants and the concentrations (10% and 5%). Evalution was performed on the speed of coagulating time and also the quality of rubber cup lumps resulted by the tested coagulants, such as water content, rubber dry weight, ash content, and also the value of sensory quality (smell, color, texture, and the bubble). Liquid smoke resulted from oil palm fronds showed the best performance due to it’s lowest pH value (2.5) and the shorthest coagulating time (40 min) at concentration of 10%, which produced rubber lumps with rubber dry weight of 99.86% and ash content of 0.83%. Meanwhile, the highest yield of liquid smoke was resulted from galam wood (10%) with coagulating time of 72 minutes.
橡胶杯块的质量是由几个因素决定的,其中一个因素是乳胶混凝剂。乳胶混凝剂的种类也会影响混凝时间。液体烟因其节省和有效的操作而成为最受推荐的乳胶混凝剂之一。Deorub是最受欢迎的液体烟,由油棕仁制成。目前仍有许多替代液体烟材料,其经济价值可能较低。本研究的目的是评估和比较三种替代材料,即甘兰木,橡胶木和油棕叶作为乳胶混凝剂生产的三种液体烟的性能。本研究采用组随机设计,两因素分别为混凝剂类型和浓度(10%和5%)。对凝固时间的速度和所测试的混凝剂所产生的橡胶杯块的质量进行了评价,如含水量、橡胶干重、灰分含量,以及感官质量(气味、颜色、质地和气泡)的价值。油棕叶液烟在浓度为10%时,pH值最低(2.5),凝结时间最短(40 min),效果最佳,胶块干重为99.86%,灰分含量为0.83%。混凝时间为72 min时,木渣液烟产率最高(10%)。
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引用次数: 0
Testing of physical properties and microbial contamination of capsule shell made from starch sago rumbia (Metroxylon sagu Rottb) and carrageenan 淀粉西米与卡拉胶胶囊壳的物性及微生物污染测试
Pub Date : 2019-10-30 DOI: 10.24111/JRIHH.V11I1.4802
Hamlan Ihsan, I. D. G. P. Prabawa, D. Harsono, Rinne Nintasari, Rina Apriani, Afandy Bayu Nurcahyo
The purpose of this study was to analyse the physical abilities and microbial contamination of modified sago starch using propylene oxide by the hydroxypropylation method. The research design used was a complete randomized design in which the fixed variable was modified propylene oxide sago starch. While the non-permanent variables used sodium tripolyphophate (STPP) at a concentration of 0.3%; 0.5%; 0.6% (b/b) and carrageenan at a concentration of 3%; 2% (b/b). This process was carried out in two stages, adding plasticizers (glycerol) and without plasticizers with a concentration of 2% (v/v). Aquadest used as a solvent with the total amount 100% (b/v). The results of testing he physical properties of the FTIR test process indicate the presence of a spectrum in the region of 3000 - 2500 cm -1 which was a specification of propylene oxide. Whereas for the solubility test of sago starch for water, acid and alike intestinal fluid it met the standards according to Farmakope Indonesia 4 th ed. Testing of microbial contamination in pathogenic bacteria types, namely E. coli, Salmonella, and Staphylococcus aureus gave negative test results, whereas for the total plate value (ALT) of sago starch capsules shells showed a value of 1.5x10 2 cfu/g.
研究了环氧丙烷羟丙基化法改性西米淀粉的物理性能和微生物污染情况。研究设计采用完全随机设计,固定变量为改性环氧丙烷西米淀粉。而非永久性变量使用浓度为0.3%的三聚磷酸钠(STPP);0.5%;0.6% (b/b),卡拉胶浓度为3%;2% (b / b)。该工艺分添加增塑剂(甘油)和不添加增塑剂(浓度为2% (v/v))两个阶段进行。使用Aquadest作为溶剂,总量为100% (b/v)。FTIR测试过程的物理性能测试结果表明,在3000 - 2500cm -1范围内存在一个光谱,这是环氧丙烷的一个规格。而西米淀粉对水、酸等肠液的溶解度检测符合印尼《Farmakope》第4版的标准。病原菌类型大肠杆菌、沙门氏菌、金黄色葡萄球菌的微生物污染检测结果为阴性,西米淀粉胶囊壳的总平板值(ALT)为1.5 × 10 2 cfu/g。
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引用次数: 1
Sifat fisik, mekanik dan akustik papan partikel berbahan dasar batang jagung (Zea mays L.) [Physical, mechanical and acoustical characteristics of particleboard made from corn stalk (Zea mays L.)]
Pub Date : 2019-10-30 DOI: 10.24111/JRIHH.V11I1.4344
L. Astari, F. A. Syamani, K. W. Prasetiyo
Corn stalk is considered as agricultural by-products that is abundance and easy to obtain in Indonesia. Corn stalk is an agricultural by-product that highly potential to be exploited as raw material for particleboard production. The research was aimed to evaluate the use of corn stalk in the production of particleboard especially for its acoustics and physical properties. Targeted board density was 0.5 g/cm 3 and 0.7 g/cm 3 . The Adhesive used in the board production were urea formaldehyde 10%, phenol formaldehyde 10% and isocyanate (pMDI) 10% based on their solid content. Board dimension was 35 x 35 x 1 cm. The pressure used was 25 kgf/cm 2 , temperature of 130℃ and 150℃ for 10 minutes. After production, boards were conditioned at room temperature for 7 days prior to testing. The standard for physical and mechanical properties JIS A 5908-2003, Acoustic properties testing was according to JIS A 1405-1963. The result shows that particleboard with isocyanate adhesive both with low and medium density had the best result. Acoustic testing shows that the highest sound absorption coefficient was given by particleboard with isocyanate at frequency 1500 Hz.
玉米秸秆被认为是农业副产品,在印度尼西亚储量丰富且容易获得。玉米秸秆是一种极具开发潜力的农业副产品,可作为生产刨花板的原料。本研究旨在评价玉米秸秆在刨花板生产中的应用,特别是其声学和物理性能。目标板密度分别为0.5 g/ cm3和0.7 g/ cm3。板材生产中使用的胶粘剂根据固体含量分别为脲醛10%、酚醛10%和异氰酸酯(pMDI) 10%。板尺寸为35 × 35 × 1厘米。使用的压力为25 kgf/ cm2,温度为130℃和150℃,持续10分钟。制作完成后,在测试前在室温条件下放置7天。物理和机械性能的标准为JIS A 5908-2003,声学性能测试按JIS A 1405-1963进行。结果表明,低密度和中密度刨花板用异氰酸酯胶粘剂处理效果最好。声学测试表明,在1500 Hz频率下,异氰酸酯刨花板的吸声系数最高。
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引用次数: 2
Produksi bio-oil dan bio-arang dari mata kayu industri pulp melalui pirolisis [Production of bio-oil and bio-char from knot in pulp mill through pyrolysis] 生物油和生物炭的生产,工业浆通过热解。
Pub Date : 2019-10-30 DOI: 10.24111/jrihh.v11i1.4325
Syamsudin Syamsudin
Acacia mangium knot is one of the biomass reject produced from the wood chemical pulping processes. This raw material is suitable for the production of bio-oil and bio-char in competitive costs. Utilization of the knot for the production of bio-oil and bio-char makes pulp mill as a bio-refining system with many profitable products because of increased income from bio-oil and bio-char and reduced costs for solid waste disposal. This study aims to evaluate the pyrolysis of knots from the kraft pulp mill to produce bio-oil and bio-char. Pyrolysis experiments of Acacia mangium knotwere carried out using laboratory-scale fluidized bed reactors at 400 o C for 30 minutes. Acacia mangium knot contains volatile matterof 69.90% (dried basis) with a calorific value of 4279 kcal/kg (dried basis) has potency to produce bio-oil through the pyrolysis process. The TG-DTG analysis with heating rate of 10 o C/ min showed the pyrolysis reaction at temperature of 200 o C-750 o C resulting in a mass decreasing from 90% to 30% or around 85% of total conversion. The yield of bio-oil from fast pyrolysis was about 47%. Bio-oil contains high various organic compounds and dominated by acetic acid (21%) and 2-propanone (28%), and produced bio-char with a calorific value of 5763 kcal/kg (dried basis). Bio-char products could be used as a solid fuel in the combustion process or gasification process.
相思结是木材化学制浆过程中产生的生物质废弃物之一。该原料适合生产成本具有竞争力的生物油和生物炭。利用该结生产生物油和生物炭,使纸浆厂成为一个具有许多有利可图产品的生物精炼系统,因为它增加了生物油和生物炭的收入,降低了固体废物处理的成本。本研究的目的是评价硫酸盐纸浆厂的节热解生产生物油和生物炭。利用实验室规模的流化床反应器,在400℃条件下进行了相思结30 min的热解实验。相思结含有69.90%的挥发物(干基),热值为4279千卡/公斤(干基),具有通过热解工艺生产生物油的潜力。升温速率为10℃/ min的TG-DTG分析表明,在200℃-750℃的温度下,热解反应导致总转化率从90%下降到30%,约为85%。快速热解的生物油收率约为47%。生物油中各种有机物含量较高,以乙酸(21%)和2-丙烷(28%)为主,生产的生物炭热值为5763 kcal/kg(干基)。生物炭产品可作为固体燃料用于燃烧过程或气化过程。
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引用次数: 0
Aplikasi ekstrak sarang semut sebagai senyawa antimikroba dan antioksidan pada permen karet herbal [Application of ant nests (Myrmecodia sp.) extract as antimicrobial and antioxidant compounds in herbal gum]
Pub Date : 2019-10-30 DOI: 10.24111/JRIHH.V11I1.4806
Nadra Khairiah, I. G. A. Prabawa, Saibatul Hamdi, Nazarni Rahmi
Ant nests (Myrmecodia sp .) are one of the medicinal plants that have many benefits for health. Ant nests are widely used as antimoicrobial, antidiabetic, anticancer and antiimplantation because ant nests usually contains chemical compounds such as flavonoids, terpenoids, saponins and polyphenol tannins. Flavonoids can be used as antioxidants, antiangiogenic, and antimicrobials. Many products can be developed from the extract, such as tea, powder and ant nest capsules. Another product innovation that needs to be developed is gum. It is important to conduct research to determine the optimum formulation of ant nest extract as an additional ingredient on candy products (gum). This study aims to determine the antimicrobial and antioxidant activities of ant nests extract and maximize the use of ant nest extractas at additional raw material for high quality gum. The ant nests were extracted using 70% ethanol. The concentrated extract was tested for antimicrobial activity in four types of pathogenic bacteriaEscherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, Pseudomonas aeruginosa ATCC 9027, and Salmonella typhimurium ATCC 14028); antimicrobial positive control was tested using chloromphenicol and antioxidant activity positive control was tested with ascorbic acid (vitamin C). Dried ant nest extract was added to the gum mixture as much as 0.15% and 3% and then the quality was tested based on the parameters according to the quality standard of gum. The results of this study showed that ant nest extract had effective antibacterial activity in gram-positive and gram negative bacteria with an inhibitory zone of 10-13.5 mm. The high antioxidant activity of ant nests (85.90%) proven that ant nests had the potential as an natural antioxidants added in food. The addition of 0.15% and 0.3% extracts in chewing gum generally produced gum that met the quality standards of chewing gum, although the moisture content was slightly higher than the maximum limit of the standards.
蚁巢(Myrmecodia sp .)是一种有益健康的药用植物。蚁巢通常含有黄酮类化合物、萜类化合物、皂苷类化合物和多酚单宁类化合物,因此被广泛用于抗菌、降糖、抗癌和抗着床。类黄酮可以用作抗氧化剂、抗血管生成剂和抗菌剂。许多产品可以从提取物开发,如茶,粉和蚁巢胶囊。另一个需要开发的创新产品是口香糖。进行研究以确定作为糖果产品(口香糖)附加成分的蚁巢提取物的最佳配方是很重要的。本研究旨在确定蚁巢提取物的抗菌和抗氧化活性,并最大限度地利用蚁巢提取物作为优质口香糖的额外原料。用70%乙醇提取蚁巢。对大肠杆菌ATCC 25922、金黄色葡萄球菌ATCC 6538、变形链球菌ATCC 25175、铜绿假单胞菌ATCC 9027、鼠伤寒沙门氏菌ATCC 14028等4种致病菌进行抑菌活性检测;以氯苯酚为抗菌阳性对照,以抗坏血酸(维生素C)为抗氧化活性阳性对照。在胶料混合物中分别添加0.15%和3%的干蚁巢提取物,根据胶料质量标准的各项参数进行质量检测。本研究结果表明,蚁巢提取物对革兰氏阳性菌和革兰氏阴性菌均有较好的抑菌活性,抑菌带为10 ~ 13.5 mm。蚁巢具有较高的抗氧化活性(85.90%),证明蚁巢具有作为天然抗氧化剂添加到食品中的潜力。在口香糖中添加0.15%和0.3%的提取物,一般可以生产出符合口香糖质量标准的口香糖,但水分含量略高于标准的最高限量。
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引用次数: 1
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Jurnal Riset Industri Hasil Hutan
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