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ISOLATION AND CHARACTERIZATION OF INULINASE ENZYME-PRODUCING MICROBES FROM BANANA FRUIT (Musa paradisiaca) 香蕉(Musa paradisiaca)果实中产胰岛素酶的微生物的分离与鉴定
Pub Date : 2024-07-01 DOI: 10.24246/agric.2024.v36.i1.p39-52
Hadistiyani Yusuf, W. Wijanarka
Inulinase (EC 3.2.1.80) is an enzyme that can hydrolyze inulin into fructose or fructo-oligosaccharides. Using the inulinase enzyme as a catalyst in making inulinase-based fructose can obtain a fructose percentage of 90-95 percent. Inulin is a linear polysaccharide (ß-2,1-linked d-fructose residue terminated by a glucose residue) that accumulates as a storage carbohydrate in plants. One natural source that can produce inulin is bananas. Bananas are a source of inulin which contains around 0.58-1.09 percent. This research used three types of bananas, including Musa X paradisiaca, Musa paradisiaca formatypica, Musa paradisiaca var sapientum. Apart from that, you can also find out the characteristics of the microbes and enzyme activity produced from the three types of bananas. The results of this research show that the three isolated types of banana are capable of producing the inulin ase enzyme which is indicated by the growth of these microbes in ISM media. ISM media (Inulinase Selective Media) is an inulinase mixing media where only inulin-producing microbes can grow on the media. The microbes isolated from the three types of bananas that had the highest inulinase enzyme activity value were Raja Nangka bananas with an enzyme activity value of 0.405 IU/mL. Meanwhile, the lowest inulinase enzyme activity value was in Kepok bananas with an enzyme activity value of 0.088 IU/mL.
菊粉酶(EC 3.2.1.80)是一种能将菊粉水解成果糖或果寡糖的酶。使用菊粉酶作为催化剂来制造菊粉基果糖,可以获得 90-95% 的果糖比例。菊粉是一种线性多糖(ß-2,1-连接的 d-果糖残基以葡萄糖残基终止),在植物中作为储存碳水化合物积累。香蕉是产生菊粉的天然来源之一。香蕉是菊粉的一种来源,其含量约为 0.58-1.09%。这项研究使用了三种香蕉,包括 Musa X paradisiaca、Musa paradisiaca formatypica 和 Musa paradisiaca var sapientum。除此以外,还可以了解这三种香蕉所产生的微生物和酶活性的特点。研究结果表明,这三种分离出来的香蕉都能产生菊粉糖酶,这从这些微生物在 ISM 培养基中的生长情况可以看出。ISM 培养基(菊粉酶选择性培养基)是一种菊粉酶混合培养基,只有产菊粉的微生物才能在培养基上生长。从三种香蕉中分离出的微生物中,菊粉酶酶活值最高的是 Raja Nangka 香蕉,酶活值为 0.405 IU/mL。而菊糖酶酶活值最低的是 Kepok 香蕉,酶活值为 0.088 IU/mL。
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引用次数: 0
FACTORS INFLUENCING AGRICULTURAL VOCATIONAL HIGH SCHOOL STUDENTS' INTEREST IN WORKING IN THE AGRICULTURAL SECTOR 影响农业职业高中学生从事农业工作兴趣的因素
Pub Date : 2024-07-01 DOI: 10.24246/agric.2024.v36.i1.p131-140
Agus Yuniawan Isyanto, Ai Tusi Fatimah, Lidya Nur Amalia
The agricultural sector is one of the sectors that makes a large contribution to labor absorption in Indonesia, however data shows a decline in the rate of labor absorption in the agricultural sector, especially among the younger generation. One effort to increase the number of young workers absorbed in the agricultural sector is through agricultural vocational school education which is expected to grow the interest of agricultural vocational school students to work in the agricultural sector in order to overcome the decline in farmer regeneration in Indonesia. The research objectives are to: (1) Identify the interest of agricultural vocational school students in agriculture to work in the agricultural sector, and (2) analyze the factors that influence agricultural vocational school students' interest in working in the agricultural sector. The research was carried out in September 2023 at one of the vocational schools specializing in agricultural skills in Ciamis Regency. The research sample consisted of 78 students who were determined using cluster random sampling. Agricultural vocational school students' interest in working in the agricultural sector was analyzed descriptively, while the factors that influenced agricultural vocational school students' interest in working in the agricultural sector were analyzed using multiple linear regression analysis. The research results show: (1) The majority of agricultural vocational school students who are interested in working in the agricultural sector are in the medium category, and (2) The social environment, community environment and social status have a significant influence on agricultural vocational school students' interest in working in the agricultural sector, while their parents' income has no significant influence.
农业部门是对印尼劳动力吸纳贡献较大的部门之一,但数据显示,农业部门的劳动力吸纳率有所下降,尤其是年轻一代。为增加农业部门吸纳年轻劳动力的数量,一种方法是通过农业职业学校教育来提高农业职业学校学生在农业部门工作的兴趣,从而克服印尼农民再生率下降的问题。研究目标是(1) 确定农业职业学校学生在农业部门工作的兴趣,以及 (2) 分析影响农业职业学校学生在农业部门工作兴趣的因素。研究于 2023 年 9 月在 Ciamis Regency 的一所农业技能职业学校进行。研究样本由 78 名学生组成,采用整群随机抽样法确定。对农业职业学校学生在农业部门工作的兴趣进行了描述性分析,并使用多元线性回归分析法对影响农业职业学校学生在农业部门工作兴趣的因素进行了分析。研究结果表明:(1) 有志于从事农业部门工作的农业职业学校学生大多数属于中等类别;(2) 社会环境、社区环境和社会地位对农业职业学校学生从事农业部门工作的兴趣有显著影响,而其父母的收入没有显著影响。
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引用次数: 0
MODELING INTENSITY OF BACTERIAL LEAF BLIGHT ON RICE PLANTS DUE TO INFLUENCE OF ABIOTIC FACTORS IN BABAKSARI VILLAGE, GRESIK 基于非生物因素影响的水稻细菌性叶枯病的模拟强度
Pub Date : 2023-08-18 DOI: 10.24246/agric.2023.v35.i1.p149-158
D. Lestari, E. Prasetyawati, Penta Suryaminarsih
Rice plants is the main food crop in Indonesia. Gresik is one of the ten highest rice producing regions in East Java Province, Indonesia. Rice production in Babaksari Village, Gresik Regency reaches 646 tonnes/year. One of the obstacles in rice production is bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. This disease can cause yield loss reaching 15-80%. The development of bacterial leaf blight in rice can be caused by several factors, one of which is abiotic factors. This study aims to determine how far the influence exerted by abiotic factors (air humidity, soil pH, and air temperature) on the intensity of bacterial leaf blight. The research was conducted using a survey method, diagonal random sampling, and interviews with farmers in Petissari Hamlet and Sariwonorejo Hamlet, Babaksari Village, Dukun District, Gresik Regency. From the results of this study it was found that the three abiotic factors simultaneously had a significant effect on the intensity of bacterial leaf blight with the regression model obtained, namely: (1) Petissari: Y = -0.050 + 0.587X1 +1.378X2 – 0.789X3; and (2) Sariwonorejo: Y = 8.464 - 3.044X1 +3.745X2 + 1.854X3. The results of path analysis showed that the abiotic factor that had the highest direct effect on the intensity of bacterial leaf blight was soil pH.
水稻是印尼的主要粮食作物。Gresik是印度尼西亚东爪哇省十大稻米产区之一。Gresik Regency Babaksari村的水稻产量达到646吨/年。水稻黄单胞菌(Xanthomonas oryzae pv)引起的细菌性叶枯病是水稻生产的障碍之一。oryzae。此病可造成15-80%的产量损失。水稻细菌性叶枯病的发生与多种因素有关,其中非生物因素是其中之一。本研究旨在确定非生物因素(空气湿度、土壤pH值和气温)对细菌性叶枯病强度的影响程度。本研究采用对角随机抽样的调查方法,对Gresik县Dukun区Babaksari村Petissari哈姆雷特和Sariwonorejo哈姆雷特的农民进行访谈。本研究结果表明,3种非生物因子同时对青枯病的发生强度有显著影响,得到的回归模型为:(1)Petissari: Y = -0.050 + 0.587X1 +1.378X2 - 0.789X3;(2) Sariwonorejo: Y = 8.464 - 3.044X1 +3.745X2 + 1.854X3。通径分析结果表明,对青枯病发生强度直接影响最大的非生物因子是土壤pH。
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引用次数: 0
THE POTENTIAL OF POTATO CULTIVATION (Solanum tuberosum L.) WITH THE APPLICATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) AND TRICHO POWDER COMMERCIAL ON MEDIUM LAND 马铃薯栽培的潜力随着促进植物生长的根瘤菌(pgpr)和毛菌粉在中等土地上的商业化应用
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p133-148
Jennefer Constantia, S. Jannah, W. Wijanarka, S. Purwantisari
The market demand for potatoes is very high, and their cultivation can affect the environmental balance, so innovation in potato yield is needed. Medium land is an area with an elevation of 300-700mdpl. Crop yields in medium land are not optimal and more susceptible to disease than highlands. The role of microorganisms in PGPR and Tricho Powder can maximize crop yields and help reduce disease intensity. This study aims to determine the effect of different concentrations of commercial PGPR in increasing the yield of potato cultivation, the effect of different concentrations of Tricho Powder in suppressing the intensity of disease attacks, and find out one species in PGPR. This research was conducted with RAL (6 treatments with 4 replications). The treatments included P0 (Farmers’ Habits), P1 (PGPR 20mL), P2 (PGPR 40 mL), P3 (Tricho Powder 80gram/10Liter), P4 (Tricho Powder 150gram/10L), and P5 (PGPR 20mL+Tricho Powder) 80gr/10L). The data was then analyzed by ANOVA. If there is significance, a follow-up test is carried out, which is determined based on the KK value. The results showed that differences in PGPR concentrations positively affected potato yields with the combination treatment of PGPR 20mL/10L+Tricho Powder 80 gr/10L (P5), which was the best treatment producing the highest mean in each parameter. In the same treatment, disease intensity was reduced by 37.55% compared to the control treatment. Based on its ability to produce IAA from 5 isolates, only 1 isolate (IS 5) showed positive results and was continued to molecular tests.
马铃薯的市场需求非常大,其种植会影响环境平衡,因此需要马铃薯产量创新。中等土地是指海拔在300-700mdpl之间的地区。中等土地的作物产量不是最优的,而且比高地更容易患病。微生物在PGPR和Tricho粉中的作用可以最大限度地提高作物产量,并有助于降低病害强度。本研究旨在确定不同浓度的商业PGPR对马铃薯增产的作用,不同浓度的Tricho粉对马铃薯病害侵袭强度的抑制作用,并找出PGPR中的一种。本研究采用RAL(6个处理,4个重复)。处理为P0(农民习惯)、P1 (PGPR 20mL)、P2 (PGPR 40ml)、P3(毛虱粉80g /10L)、P4(毛虱粉150g /10L)、P5 (PGPR 20mL+毛虱粉)80g /10L。然后对数据进行方差分析。如果有显著性,则进行后续检验,根据KK值确定。结果表明,PGPR 20mL/10L+Tricho Powder 80 gr/10L (P5)组合处理的PGPR浓度差异对马铃薯产量有正向影响,且各参数平均值最高。在相同的治疗中,疾病强度比对照治疗降低了37.55%。根据5株分离株的产IAA能力,只有1株(IS 5)显示阳性结果,并继续进行分子检测。
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引用次数: 0
ICE CREAM CONE FORMULATIONS FROMWHEAT FLOUR AND TOFU DREGS FLOUR ADDED WITH BEETROOT (Beta vulgaris L.) EXTRACT 小麦粉、豆腐渣、面粉加甜菜根的冰淇淋甜筒配方提取
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p85-98
Safitri Nur Fatmawati, V. Nur’aini, M. Karyantina
Ice cream cones are a type of wafer with a cone or funnel-like shape used as a container that holds ice cream and baked in an ice cream cone maker with the basic ingredient of wheat flour, which can be added with other ingredients for innovation. A factorial completely randomized design (CRD) was employed with two factors: the ratio of wheat flour to tofu dregs flour (90:10, 80:20, 70:30) and the percentage of beetroot extract in 100 ml of water (30, 40, 50%). This study aimed to determine which ice cream cone formulation(s) from wheat flour, tofu dregs flour, and beetroot (Beta vulgaris L.) extract had the highest protein and betacyanin content and was most favored by consumers. Results showed that formulation with the 70:30 flour ratio and 30% beetroot extract produced the highest protein content of 13.55%, and the 90:10 flour ratio and 50% beetroot extract created cones with the highest betacyanin content of 0.074 mg/100g. The product preference test based on organoleptic properties revealed that the panelists mostly preferred cones made with the 80:20 flour ratio and 30% beetroot extract because of the resulting color (3.53, like slightly), taste (3.61, like slightly), aroma (3.78, like slightly), and crispness (4.32, like), with an overall preference of 4.07 (like).
冰淇淋甜筒是一种以小麦粉为基本原料,在冰淇淋甜筒机中烘烤的圆筒状或漏斗状的威化物,可以加入其他原料进行创新。采用全随机因子设计(CRD),分别考虑小麦粉与豆腐渣粉的比例(90:10、80:20、70:30)和甜菜根提取物在100 ml水中的比例(30、40、50%)。本研究旨在确定小麦粉、豆腐渣粉和甜菜根提取物中哪一种冰淇淋蛋筒的蛋白质和β花青素含量最高,最受消费者青睐。结果表明,当面粉比例为70:30、甜菜根提取物比例为30%时,球果蛋白含量最高,为13.55%;当面粉比例为90:10、甜菜根提取物比例为50%时,球果中甜菜青素含量最高,为0.074 mg/100g。基于感官特性的产品偏好测试显示,小组成员最喜欢用80:20的面粉比例和30%的甜菜根提取物制成的锥体,因为得到的颜色(3.53,喜欢轻微),味道(3.61,喜欢轻微),香气(3.78,喜欢轻微)和脆度(4.32,喜欢),总体偏好为4.07(喜欢)。
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引用次数: 0
MORPHOLOGICAL CHARACTERISTIC OF VIERUL LOCAL GRAPE (Vitis spp.) CUTTING SEEDS ON PODZOLIC SOIL USING VARIOUS CONCENTRATION OF AUXIN SOAK 不同浓度生长素浸泡在灰化土上对当地红葡萄切花种子形态的影响
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p115-132
F. Sukmawati, Saktiyono Sigit Tri Pamungkas, Tusrianto Tusrianto, A. Laila, Y. Pramudya
The development of grapes (Vitis vinifera) has obstacle among others are limited optimal land and difficult nurseries. Suboptimal land use for grape cuttings can use red yellow podzolic soil (ultisol). Podzolic soil has an acidic pH and low macro and micro nutrient uptake but can still be used as a growing medium. Vierul local grapes are a type of grape that has begun to be developed as root stock in nurseries but needs to be improved for its root success using a synthetic phytohormones bath of napthaline acetic acid (NAA). The purpose of this study was to determine the morphological character of vierul local grape cuttings at various concentrations of NAAsoaking in podzolic soil growing medium. This research has been carried out in a screen house from August to December 2021. This study used a non-factorial completely randomized design (CRD) with 3 treatments and 3 replications as block consisting of F0, F1, F2, F4 following by 0, 2, 4, 6 g L-1 respectively. The morphological characteristics observed is number of bud break, time of leaves appear, number of leaves, tendril length and percentage of live cuttings. The results of the study were analyzed using ANOVA at the 5% level and continued using the DMRT at the 5% level. The result showed that soaking of NAA at a concentration of 0 g L-1 (F0) had the best result for the variable number of bud break and number of leaves. Concentration of 6 g L-1 (F3) had the best result for variable length of the tendril while for the variable when the leaves appear, NAA soaking has no significant effect. The percentage of live cuttings is still low because it is influenced by the quality (material) of cuttings, the age of woodstock, growing media and water availability.
葡萄(Vitis vinifera)的发展面临着最佳土地有限和苗圃困难等障碍。葡萄扦插的次优土地利用可采用红黄灰化土(多效土)。灰化土pH值偏酸性,宏微量养分吸收量低,但仍可作为生长介质。恶性本地葡萄是一种已经开始在苗圃中作为砧木培育的葡萄品种,但需要利用萘乙酸(NAA)合成植物激素浴对其进行改良才能成功生根。本研究的目的是研究不同浓度naasas浸泡在灰化土生长介质中的本地葡萄扦插的形态特征。本研究于2021年8月至12月在一家电影院进行。本研究采用非因子完全随机设计(CRD), 3个处理,3个重复,分别为F0、F1、F2、F4,分别添加0、2、4、6 g L-1。观察其形态特征为芽断数、出叶时间、叶数、卷须长度和活插枝率。研究结果在5%水平上使用方差分析,在5%水平上继续使用DMRT。结果表明,NAA浸泡浓度为0 g L-1 (F0)时,芽断数和叶片数的变化效果最好。6 g L-1 (F3)浸泡对卷须变长效果最好,对出叶时间无显著影响。活插穗的百分比仍然很低,因为它受到插穗质量(材料)、伍德斯托克树龄、生长介质和水供应的影响。
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引用次数: 0
ANALYSIS OF THE INFLUENCE OF PRODUCTION FACTORS ON THE PRODUCTION OF CASSAVA FARMING BUSINESS IN SALATIGA CITY 生产要素对萨拉提加市木薯种植业生产的影响分析
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p73-84
Ertris Taqwdas Widyastiara, E. Prasetyo, B. Kristanto
Cassava farming in Salatiga City is a food-crop commodity farming business that has good opportunities because the demand for cassava in the market is increasing. However, some obstacles hinder cassava farming, namely cassava productivity which has not reached the target and limited production factors. This indicates that the use of production factors is still not efficient. Production factors that directly affect the productivity of cassava are land area, seeds, fertilizers, and labor. The aims of this study were: (1) to analyze the influence of production factors on cassava production, (2) to analyze the level of elasticity of cassava production factors, and (3) to analyze the level of technical efficiency and economic efficiency in cassava farming activities in Salatiga City. The analytical method used is the Cobb-Douglas production function with thehelp of the SPSS program to examine the influence of factors on cassava production and production elasticity. Technical efficiency is analyzed using the stochastic frontier production function. The results showed that the factors of seeds, manure, and labor were thought to affect cassava farming. The results of the elasticity analysis of all factors of production are in the rational area (Region II) with a positive value. The results of the technical efficiency analysis show that only the seed variable has a positive and significant value. Based on the results of the calculation of  economic efficiency analysis, it shows that the input use of the land area, seeds, manure, and labor is not efficient.
萨拉蒂加市的木薯种植是一种粮食作物商品农业,由于市场对木薯的需求正在增加,因此有很好的机会。然而,木薯种植存在一些障碍,即木薯生产力未达到目标,生产要素有限。这说明生产要素的利用效率仍然不高。直接影响木薯生产力的生产要素有土地面积、种子、肥料和劳动力。本研究的目的是:(1)分析生产要素对木薯生产的影响;(2)分析木薯生产要素的弹性水平;(3)分析萨拉蒂加市木薯种植活动的技术效率和经济效率水平。采用的分析方法是Cobb-Douglas生产函数,借助SPSS程序来检验各因素对木薯生产和生产弹性的影响。采用随机前沿生产函数对技术效率进行了分析。结果表明,种子、肥料和劳动力因素被认为是影响木薯种植的因素。各生产要素的弹性分析结果均在合理区域(区域II),且均为正值。技术效率分析结果表明,只有种子变量具有正显著值。根据经济效率分析的计算结果,表明土地面积、种子、粪肥和劳动力的投入使用效率不高。
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引用次数: 0
MARKETING STRATEGY ANALYSIS OF HYDROPONIC VEGETABLES OF KEBUN SEHATI kebun sehati水培蔬菜营销策略分析
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p13-26
R. Lantarsih, Wiguna Jaelawijaya, Kadarso Kadarso, Candarisma Dhanes Noor Viana, Sulistiya Sulistiya
E-commerce has now also penetrated agricultural products, including hydroponic vegetables. This study aims to determine the marketing mix strategy of hydroponic vegetables at Kebun Sehati that uses e-commerce in its marketing. Kebun Sehati as a newcomer in hydroponic vegetable e-commerce in Yogyakarta of course has strengths and, weaknesses, and has opportunities and threats in running its business. The marketing mix which includes the product, price, place, and promotion is an important factor in this business. This research is descriptive. Respondents in this study consisted of the management of Kebun Sehati who was taken by the census method, and the determination of the consumer sample was carried out by the accidental sampling method. The total number of samples in the study was 85 respondents. The analytical method to determine the strategy of developing a hydroponic vegetable marketing mix in Kebun Sehati uses SWOT analysis. From the results of this study, it can be concluded that the right strategy for the development of hydroponic vegetable marketing at Kebun Sejati is an aggressive strategy which includes: (1) Increasing product variety and freshness, as well as providing guarantees by utilizing community assistance to expand the market; (2) Setting prices that are affordable and following quality to expand the market; (3) Using applications in the play store to serve consumers who are information technology literate; (4) Conduct promotions with complete attributes on various social media to increase consumer awareness of healthy food ingredients.   Keywords: E-commerce, hydroponic vegetables, marketing mix, SWOT analysis
电子商务现在也渗透到农产品中,包括水培蔬菜。本研究旨在确定利用电子商务进行营销的Kebun Sehati水培蔬菜的营销组合策略。Kebun Sehati作为日惹水培蔬菜电子商务的新手,当然有优势和劣势,在经营业务方面有机会和威胁。包括产品、价格、地点和促销在内的营销组合是这项业务的重要因素。这项研究是描述性的。本研究的调查对象为Kebun Sehati的管理人员,采用人口普查法,消费者样本的确定采用偶然抽样法。该研究的样本总数为85人。确定Kebun Sehati开发水培蔬菜营销组合策略的分析方法使用SWOT分析。从研究结果可以得出,Kebun Sejati发展水培蔬菜营销的正确策略是积极进取的策略,包括:(1)增加产品品种和新鲜度,并利用社区援助提供保障,扩大市场;(2)定价合理,追求质量,开拓市场;(3)利用游戏商店中的应用程序服务于具有信息技术素养的消费者;(4)在各类社交媒体上进行属性完整的推广活动,提高消费者对健康食品配料的认知。关键词:电子商务,水培蔬菜,营销组合,SWOT分析
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引用次数: 0
EFFECT INTERACTION OF SULFATE (SO4 2-) DAN CHROMATE (CrO4 2-) ON GROWTHAND CR6+ACCUMULATION IN Tagetes erecta L. 硫酸盐(so42 -)和铬酸盐(cro42 -)互作对万寿菊生长和CR6+积累的影响。
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p45-60
S. Kasmiyati, Gabriella L. Mataradja
Chromium is a heavy metal that is toxic, bioaccumulative, persistent, and cannot be decomposed in the environment. The Cr6+ ion is a chromium ion that has the highest toxicity among other chromium ions, so its presence in the environment is hazardous for plants, animals, and humans. Sulfur is an essential element for plants, whose presence can reduce Cr6+ concentrations in the terrestrial environment by inhibiting the uptake of Cr6+ by plants. Tagetes erecta is a bioremediation agent plant that can remediate land polluted with Cr6+ waste. In this research, we studied the growth of T. erecta in media polluted with Cr6+ and sulfate and its ability to accumulate of Cr6+. Several parameters observe in this experiment, i.e. growth parameters, namely plant height, number of leaves, number of flowers measured at the end of the study, dry weight, root length, and Cr6+ concentration estimated using the diphenylcarbasid method. The results showed that the plant height of T. erecta. was not significantly different, and sulfate addition did not affect plant height. The addition of sulfate affected the number of leaves and flowers, dry weight of root shoots, root length, and Cr6+ content in T. erecta. polluted with Cr6+. so that in these parameters, there was a significant difference in the addition of Cr6+ and sulfate on growth of T. erecta and its ability to Cr accumulation.
铬是一种有毒、具有生物蓄积性、持久性、不能在环境中分解的重金属。Cr6+离子是铬离子中毒性最高的一种,因此它在环境中的存在对植物、动物和人类都是有害的。硫是植物的必需元素,它的存在可以抑制植物对Cr6+的吸收,从而降低陆地环境中Cr6+的浓度。万寿菊(Tagetes erecta)是一种生物修复剂植物,可以修复土壤被Cr6+污染的废弃物。本研究研究了T. erecta在受Cr6+和硫酸盐污染的培养基中的生长及其对Cr6+的积累能力。本实验观察了几个参数,即生长参数,即株高、叶数、研究结束时测量的花数、干重、根长、二苯基羧基法估计的Cr6+浓度。结果表明,直立木的株高与直立木的株高呈正相关。差异不显著,且添加硫酸盐对株高无影响。硫酸盐的添加影响了直立柽柳叶片和花的数量、根茎干重、根长和Cr6+含量。被Cr6+污染。因此,在这些参数中,添加Cr6+和硫酸盐对直立毛霉生长和Cr积累能力的影响存在显著差异。
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引用次数: 0
MORPHOLOGICAL CHARACTERISTICS OF ORNAMENTAL CHILLI MUTANTS IRRADIATED BY GAMMA RAY 伽玛射线辐照观赏辣椒突变体的形态特征
Pub Date : 2023-08-16 DOI: 10.24246/agric.2023.v35.i1.p159-168
Zulfa Ulinnuha, N. Farid, I. Dinuriah
Chili is used not only as a vegetable for consumption or a complement to dishes but also as an attractive ornamental plant to cultivate. One of the genetic enhancement efforts is through mutation induction with gamma rays. The aim of this study was to increase the genetic diversity of ornamental chilies by inducing gamma ray mutations. This research was conducted at BATAN (National Nuclear Energy Agency), experimental field and Plant Breeding and Biotechnology Laboratory, Faculty of Agriculture, Jenderal Soedirman University. The research was conducted from July to November 2022. The results showed that the LD50 value of gamma-ray irradiated chili seeds was 213.49 Gy, and there was an increase in the diversity of ornamental chili mutants. The color characteristic of mature leaves was purplish-green in the control plant, while the mutant turned purple on the upper and lower surfaces of the leaves. The character of the flower has not changed; that is, it remains purple, and the flower stalk remains upright. The stem pigment in control plants was a purple-green line, changed to purple in mutants. Mutant plant habitus changed to compact. These characters increase the aesthetic value of the ornamental chili mutant.
辣椒不仅作为蔬菜食用或菜肴的补充,而且作为一种有吸引力的观赏植物来种植。其中一种基因增强是通过伽马射线诱导突变。本研究的目的是通过诱导γ射线突变来增加观赏辣椒的遗传多样性。这项研究是在BATAN(国家核能机构)试验场和Jenderal Soedirman大学农学院植物育种和生物技术实验室进行的。该研究于2022年7月至11月进行。结果表明,γ射线辐照辣椒种子的LD50值为213.49 Gy,观赏辣椒突变体多样性增加。对照植株成熟叶片的颜色特征为紫绿色,而突变体叶片的上下表面呈紫色。花的特性没有改变;也就是说,它保持紫色,花茎保持直立。对照植株的茎色素呈紫绿色,突变体的茎色素呈紫色。突变株习性转变为致密型。这些性状增加了观赏辣椒突变体的审美价值。
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引用次数: 0
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