Marine Life Science & Technology最新文献_第3页

Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment 威尼斯泻湖表层沉积物细菌的基因组解析宏基因组学揭示了在受影响的环境中具有高生物合成潜力和代谢可塑性的成功策略

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-11-03 DOI: 10.1007/s42995-023-00192-z

Elisa Banchi, Erwan Corre, Paola Del Negro, Mauro Celussi, Francesca Malfatti

Abstract Bacteria living in sediments play essential roles in marine ecosystems and deeper insights into the ecology and biogeochemistry of these largely unexplored organisms can be obtained from ‘omics’ approaches. Here, we characterized metagenome-assembled-genomes (MAGs) from the surface sediment microbes of the Venice Lagoon (northern Adriatic Sea) in distinct sub-basins exposed to various natural and anthropogenic pressures. MAGs were explored for biodiversity, major marine metabolic processes, anthropogenic activity-related functions, adaptations at the microscale, and biosynthetic gene clusters. Starting from 126 MAGs, a non-redundant dataset of 58 was compiled, the majority of which (35) belonged to (Alpha- and Gamma-) Proteobacteria. Within the broad microbial metabolic repertoire (including C, N, and S metabolisms) the potential to live without oxygen emerged as one of the most important features. Mixotrophy was also found as a successful lifestyle. Cluster analysis showed that different MAGs encoded the same metabolic patterns (e.g . , C fixation, sulfate oxidation) thus suggesting metabolic redundancy. Antibiotic and toxic compounds resistance genes were coupled, a condition that could promote the spreading of these genetic traits. MAGs showed a high biosynthetic potential related to antimicrobial and biotechnological classes and to organism defense and interactions as well as adaptive strategies for micronutrient uptake and cellular detoxification. Our results highlighted that bacteria living in an impacted environment, such as the surface sediments of the Venice Lagoon, may benefit from metabolic plasticity as well as from the synthesis of a wide array of secondary metabolites, promoting ecosystem resilience and stability toward environmental pressures.

生活在沉积物中的细菌在海洋生态系统中起着至关重要的作用，通过“组学”方法可以更深入地了解这些大部分未被探索的生物的生态学和生物地球化学。在这里，我们从威尼斯泻湖(亚得里亚海北部)暴露于各种自然和人为压力的不同子盆地的表面沉积物微生物中表征了宏基因组组装基因组(MAGs)。探讨了mag的生物多样性、主要海洋代谢过程、人类活动相关功能、微尺度适应和生物合成基因簇。从126个MAGs开始，编译了58个非冗余数据集，其中大部分(35个)属于(Alpha-和Gamma-) Proteobacteria。在广泛的微生物代谢库(包括碳、氮和硫代谢)中，无氧生存的潜力成为最重要的特征之一。混合营养也被认为是一种成功的生活方式。聚类分析表明，不同的mag编码相同的代谢模式(例如;C固定，硫酸盐氧化)，因此表明代谢冗余。抗生素和有毒化合物的抗性基因是偶联的，这种情况可以促进这些遗传性状的传播。MAGs在抗菌和生物技术、生物防御和相互作用以及微量营养素吸收和细胞解毒的适应策略方面显示出很高的生物合成潜力。我们的研究结果强调，生活在受影响环境中的细菌，如威尼斯泻湖的表面沉积物，可能受益于代谢可塑性以及各种次生代谢物的合成，促进生态系统对环境压力的恢复力和稳定性。

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引用次数: 0

Mucosal immune responses to Ichthyophthirius multifiliis in the ocular mucosa of rainbow trout (Oncorhynchus mykiss, Walbaum), an ancient teleost fish 虹鳟(Oncorhynchus mykiss, Walbaum)眼黏膜对多filiis鱼的免疫反应。虹鳟是一种古老的硬骨鱼

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-10-31 DOI: 10.1007/s42995-023-00199-6

Weiguang Kong, Guangyi Ding, Gaofeng Cheng, Peng Yang, Zhen Xu

Abstract The eye, as a specialized visual organ, is directly exposed to the external environment, and, therefore, it faces constant challenges from external pathogenic organisms and toxins. In the ocular mucosa (OM) of mammals, mucosal-associated lymphoid tissues (MALTs) constitute the primary line of defense. However, the immune defense role of the OM remains unknown in aquatic vertebrates. To gain insights into the immune processes within the OM of teleost fish, we developed an infection model of rainbow trout ( Oncorhynchus mykiss ) OM using a parasite, Ichthyophthirius multifiliis (Ich). Immunofluorescence, qPCR, and H&E staining revealed that Ich successfully infiltrates the OM of rainbow trout, leading to pathological structural changes, as evidenced by A&B staining. Importantly, the qPCR results indicate an up-regulation of immune-related genes following Ich infection in the OM. Moreover, transcriptome analyses were conducted to detect immune responses and impairments in eye function within the OM of rainbow trout with Ich infection. The results of the transcriptome analysis that Ich infection can cause an extensive immune response in the OM, ultimately affecting ocular function. To the best of our knowledge, our findings represent for the first time that the teleost OM could act as an invasion site for parasites and trigger a strong mucosal immune response to parasitic infection.

眼睛作为一种特殊的视觉器官，直接暴露于外部环境中，因此不断面临来自外部致病生物和毒素的挑战。在哺乳动物的眼粘膜(OM)中，粘膜相关淋巴组织(MALTs)构成了主要的防线。然而，在水生脊椎动物中，OM的免疫防御作用尚不清楚。为了深入了解硬骨鱼OM内的免疫过程，我们利用虹鳟鱼OM的寄生虫Ichthyophthirius multifiliis (Ich)建立了虹鳟鱼OM的感染模型。免疫荧光、qPCR和H&E染色显示Ich成功浸润虹鳟鱼OM，导致病理结构改变，A&B染色证实。重要的是，qPCR结果表明，Ich感染后OM中免疫相关基因的上调。此外，我们还进行了转录组分析，以检测虹鳟鱼感染Ich后眼膜内的免疫反应和眼功能损伤。转录组分析结果表明，Ich感染可引起眼内广泛的免疫反应，最终影响眼功能。据我们所知，我们的研究结果首次表明硬骨鱼OM可能作为寄生虫的入侵部位，并引发对寄生虫感染的强烈粘膜免疫反应。

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引用次数: 0

Functional evidence that FGFR regulates MAPK signaling in organizer specification in the gastropod mollusk Lottia peitaihoensis FGFR在腹足类软体动物中调控MAPK信号传导的功能证据

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-10-21 DOI: 10.1007/s42995-023-00194-x

Sujian Tan, Pin Huan, Baozhong Liu

引用次数: 0

Linking coral fluorescence phenotypes to thermal bleaching in the reef-building Galaxea fascicularis from the northern South China Sea 南海北部造礁星系中珊瑚荧光表型与热白化的关联

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-10-18 DOI: 10.1007/s42995-023-00190-1

Sanqiang Gong, Jiayuan Liang, Gang Li, Lijia Xu, Yehui Tan, Xinqing Zheng, Xuejie Jin, Kefu Yu, Xiaomin Xia

Abstract Coral fluorescence phenotypes have been suggested as an adaptation to a broad range of environmental conditions, yet the mechanisms linking thermal bleaching tolerance in reef-building coral populations, associated with fluorescence phenotypes due to GFP-like proteins, remains unclear. In this study, the relationship between the thermal sensitivity and phenotypic plasticity of corals was investigated using two phenotypes of Galaxea fascicularis, green and brown. The results reveal that brown G. fascicularis was more susceptible to bleaching than green G. fascicularis when exposed to a higher growth temperature of 32 °C. Both phenotypes of G. fascicularis were associated with the thermotolerant Symbiodiniaceae symbiont, Durusdinium trenchii . However, the brown G. fascicularis showed a significant decrease in Symbiodiniaceae cell density and a significant increase in pathogenic bacteria abundance when the growth temperature was raised from 29 to 32 °C. The physiological traits and transcriptomic profiles of Symbiodiniaceae were not notably affected, but there were differences in the transcriptional levels of certain genes between the two phenotype hosts of G. fascicularis. Under heat stress of 32 °C, the gene encoding green fluorescent protein (GFP)-like and chromosome-associated proteins, as well as genes related to oxidative phosphorylation, cell growth and death showed lower transcriptional levels in the brown G. fascicularis compared to the green G. fascicularis. Overall, the results demonstrate that the green form of G. fascicularis is better able to tolerate ocean warming and defend against pathogenic bacteria, likely due to higher gene transcription levels and defense ability.

珊瑚的荧光表型被认为是对广泛的环境条件的适应，但造礁珊瑚种群的热漂白耐受性与gfp样蛋白引起的荧光表型之间的联系机制尚不清楚。本研究以束状Galaxea fascularis的绿色和棕色两种表型为研究对象，研究了珊瑚的热敏性与表型可塑性之间的关系。结果表明，在32℃的较高生长温度下，棕色束草比绿色束草更容易发生白化;这两种表型都与耐高温的共生菌Durusdinium trenchii有关。而当生长温度从29℃升高到32℃时，褐束草共生菌科细胞密度显著降低，病原菌丰度显著增加。共生菌科植物的生理性状和转录组学特征未受显著影响，但两种表型寄主间某些基因的转录水平存在差异。在32°C的热应激下，编码绿色荧光蛋白(GFP)样蛋白和染色体相关蛋白的基因，以及与氧化磷酸化、细胞生长和死亡相关的基因在棕色卷叶草中的转录水平低于绿色卷叶草。总体而言，结果表明，绿色形式的束状海参能够更好地耐受海洋变暖并防御致病菌，这可能是由于更高的基因转录水平和防御能力。

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引用次数: 0

Towards disentangling the classification of freshwater fish trypanosomes 淡水鱼锥虫分类的厘清

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-10-13 DOI: 10.1007/s42995-023-00191-0

Peng Zhang, Jie Liu, Xiao-Ming Yin, Jun-Yu Zhou, Julius Lukeš, Zhao-Rong Lun, De-Hua Lai

Abstract Currently, new species of freshwater fish trypanosomes, which are economically important parasites, are being described based on subjectively selected features, i.e., their cell morphology and the host species. We have performed detailed phylogenetic and haplotype diversity analyses of all 18S rRNA genes available for freshwater fish trypanosomes, including the newly obtained sequences of Trypanosoma carassii and Trypanosoma danilewskyi . Based on a sequence similarity of 99.5%, we divide these trypanosomes into 15 operational taxonomic units, and propose three nominal scenarios for distinguishing T. carassii and other aquatic trypanosomes. We find evidences for the existence of a low number of freshwater fish trypanosomes, with T. carassii having the widest geographic and host ranges. Our analyses support the existence of an umbrella complex composed of T. carassii and two sister species.

摘要淡水鱼锥虫是一种重要的经济寄生虫，目前人们根据其细胞形态和寄主种类等主观选择的特征对其新种进行了描述。我们对淡水鱼锥虫所有可用的18S rRNA基因进行了详细的系统发育和单倍型多样性分析，包括新获得的卡拉西锥虫和丹尼莱夫斯基锥虫序列。基于99.5%的序列相似性，我们将这些锥虫划分为15个操作分类单元，并提出了3种区分卡拉西锥虫与其他水生锥虫的名义情景。我们发现了淡水鱼锥虫存在数量较少的证据，其中卡拉西锥虫具有最广泛的地理和宿主范围。我们的分析支持由T. carassii和两个姐妹种组成的保护伞复合体的存在。

引用次数: 1

Correction: Incorporating mesopelagic fish into the evaluation of marine protected areas under climate change scenarios 更正:将中上层鱼类纳入气候变化情景下海洋保护区的评估

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-10-13 DOI: 10.1007/s42995-023-00193-y

Shuhao Liu, Yang Liu, Katharina Teschke, Mark A. Hindell, Rachel Downey, Briannyn Woods, Bin Kang, Shuyang Ma, Chi Zhang, Jianchao Li, Zhenjiang Ye, Peng Sun, Jianfeng He, Yongjun Tian

引用次数: 0

Contribution of the SOS response and the DNA repair systems to norfloxacin induced mutations in E. coli SOS反应和DNA修复系统对诺氟沙星诱导的大肠杆菌突变的贡献

2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-09-21 DOI: 10.1007/s42995-023-00185-y

Tongtong Lin, Jiao Pan, Colin Gregory, Yaohai Wang, Clayton Tincher, Caitlyn Rivera, Michael Lynch, Hongan Long, Yu Zhang

Abstract Antibiotic-resistant bacteria severely threaten human health. Besides spontaneous mutations generated by endogenous factors, the resistance might also originate from mutations induced by certain antibiotics, such as the fluoroquinolones. Such antibiotics increase the genome-wide mutation rate by introducing replication errors from the SOS response pathway or decreasing the efficiency of the DNA repair systems. However, the relative contributions of these molecular mechanisms remain unclear, hindering understanding of the generation of resistant pathogens. Here, using newly-accumulated mutations of wild-type and SOS-uninducible Escherichia coli strains, as well as those of the strains deficient for the mismatch repair (MMR) and the oxidative damage repair pathways, we find that the SOS response is the major mutagenesis contributor in mutation elevation, responsible for ~ 30–50% of the total base-pair substitution (BPS) mutation-rate elevation upon treatment with sublethal levels of norfloxacin (0 ~ 50 ng/mL). We further estimate the significance of the effects on other mutational features of these mechanisms (i.e., transversions, structural variations, and mutation spectrum) in E. coli using linear models. The SOS response plays a positive role in all three mutational features (mutation rates of BPSs, transversions, structural variations) and affects the mutational spectrum. The repair systems significantly reduce the BPS mutation rate and the transversion rate, regardless of whether antibiotics are present, while significantly increasing the structural variation rate in E. coli . Our results quantitatively disentangle the contributions of the SOS response and DNA repair systems in antibiotic-induced mutagenesis.

摘要耐药细菌严重威胁着人类健康。除了内源性因素引起的自发突变外，耐药性也可能源于某些抗生素(如氟喹诺酮类药物)引起的突变。这类抗生素通过引入来自SOS反应途径的复制错误或降低DNA修复系统的效率来增加全基因组突变率。然而，这些分子机制的相对贡献仍然不清楚，阻碍了对耐药病原体产生的理解。在这里，利用野生型和SOS不可诱导型大肠杆菌菌株的新积累突变，以及缺乏错配修复(MMR)和氧化损伤修复途径的菌株，我们发现SOS反应是突变升高的主要诱变因素，在亚致死水平的诺氟沙星(0 ~ 50 ng/mL)处理下，约30-50%的总碱基对取代(BPS)突变率升高负责。我们使用线性模型进一步估计了这些机制对大肠杆菌中其他突变特征(即，翻转，结构变异和突变谱)的影响的重要性。SOS反应在bps的三个突变特征(突变率、翻转、结构变异)中都起着积极的作用，并影响突变谱。无论是否存在抗生素，修复系统都显著降低了BPS突变率和翻转率，同时显著增加了大肠杆菌的结构变化率。我们的结果定量地解开了SOS反应和DNA修复系统在抗生素诱导诱变中的贡献。

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引用次数: 1

On four epibiotic peritrichous ciliates (Protozoa, Ciliophora) found in Lake Weishan Wetland: morphological and molecular data support the establishment of a new genus, Parapiosoma gen. nov., and two new species. 在微山湖湿地发现的四种表生性缘毛纤毛虫（原生动物、西里奥虫）：形态学和分子数据支持建立一个新属Parapiosoma gen.nov.和两个新种。

IF 5.7 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-08-22 eCollection Date: 2023-08-01 DOI: 10.1007/s42995-023-00184-z

Tong Wu, Ting Cheng, Xiao Cao, Yaohan Jiang, Khaled A S Al-Rasheid, Alan Warren, Zhe Wang, Borong Lu

During a study on the diversity of ciliated protists in Lake Weishan Wetland, the largest wetland in northern China, four epibiotic sessilid peritrichs were isolated from aquatic host animals. Two of them, i.e., Epistylis cambari Kellicott, 1885 and Epistylis lwoffi Fauré-Fremiet, 1943, were known species whereas the other two, i.e., Parapiosoma typicum gen. nov., sp. nov. and Orborhabdostyla gracilis sp. nov., are new to science. The new genus Parapiosoma gen. nov. is characterized by its branched non-contractile stalk, everted peristomial lip, obconical macronucleus and transverse silverlines. Two species are assigned to the new genus, namely Parapiosoma typicum sp. nov. and Parapiosoma gasterostei (Fauré-Fremiet, 1905) comb. nov. Morphologically, P. typicum sp. nov. is recognized by its goblet-shaped zooids, single-layered peristomial lip, dichotomously branched stalk, and infundibular polykinety 3 (P3) containing three equal-length rows. Orborhabdostyla gracilis sp. nov. is characterized by its slender zooid, curved macronucleus, and three equal-length rows in infundibular P3. Improved diagnoses and redescriptions of E. cambari and E. lwoffi are provided including, for the first time, data on the ciliature of E. cambari. Phylogenetic analyses based on SSU rDNA, ITS1-5.8S rDNA -ITS2, and LSU rDNA sequence data strongly support the assertion that the family Epistylididae comprises morphospecies with different evolutionary lineages and indicate that Parapiosoma gen. nov. may represent a new taxon at family level.

在对中国北方最大湿地微山湖湿地纤毛虫原生生物多样性的研究中，从水生宿主动物中分离到4种表生无柄轮藻。其中两个是已知物种，即1885年的Epistylis cambari Kellicott和1943年的Epistylis lwoffi Fauré-Fremiet，而另外两个是新发现的物种，即Parapiosoma typicum gen.nov.，sp.nov.和Orborhabdostyla gracilis sp.nov。新属Parapiosoma gen.nov.的特征是其分枝的不收缩茎、外翻的吻周唇、倒圆锥形大核和横向银纹。两个物种被归入新属，即Parapiosoma typicum sp.nov.和Parapiosomagasterostei（Fauré-Fremiet，1905）comb。从形态学上看，P.typicum sp.nov.是由其杯状的动物群、单层的吻周唇、二歧分枝的柄和包含三个等长行的漏斗多聚性3（P3）所识别的。Orborhabdostyla gracilis sp.nov.的特征是其细长的动物体、弯曲的大核和漏斗P3中的三排等长。提供了对E.cambari和E.lwoffi的改进诊断和重新描述，包括首次提供了关于E.cambary纤毛虫的数据。基于SSU rDNA、ITS1-5.8S rDNA-ITS2和LSU rDNA序列数据的系统发育分析有力地支持了上突虫科由具有不同进化谱系的形态物种组成的断言，并表明新一代Parapiosoma可能代表了一个新的科级分类单元。

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引用次数: 0

Large yellow croaker (Larimichthys crocea) mitofusin 2 inhibits type I IFN responses by degrading MAVS via enhanced K48-linked ubiquitination. 大黄鱼线粒体融合蛋白2通过增强K48连接的泛素化降解MAVS来抑制I型IFN反应。

IF 5.8 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-08-18 eCollection Date: 2023-08-01 DOI: 10.1007/s42995-023-00189-8

Wen-Xing Li, Xiao-Hong Wang, Yi-Jun Lin, Yuan-Yuan Zhou, Jun Li, Xiang-Yang Zhang, Xin-Hua Chen

In mammals, mitofusin 2 (MFN2) is involved in mitochondrial fusion, and suppresses the virus-induced RIG-I-like receptor (RLR) signaling pathway. However, little is known about the function of MFN2 in non-mammalian species. In the present study, we cloned an MFN2 ortholog (LcMFN2) in large yellow croaker (Larimichthys crocea). Phylogenetic analysis showed that MFN2 emerged after the divergence of amphioxus and vertebrates. The protein sequences of MFN2 were well conserved from fish to mammals. LcMFN2 was expressed in all the tissues/organs examined at different levels, and its expression was upregulated in response to poly(I:C) stimulation. Overexpression of LcMFN2 inhibited MAVS-induced type I interferon (IFN) promoter activation and antiviral gene expression. In contrast, knockdown of endogenous LcMFN2 enhanced poly(I:C) induced production of type I IFNs. Additionally, LcMFN2 enhanced K48-linked polyubiquitination of MAVS, promoting its degradation. Also, overexpression of LcMFN2 impaired the cellular antiviral response, as evidenced by the increased expression of viral genes and more severe cytopathic effects (CPE) in cells infected with spring viremia of carp virus (SVCV). These results indicated that LcMFN2 inhibited type I IFN response by degrading MAVS, suggesting its negative regulatory role in cellular antiviral response. Therefore, our study sheds a new light on the regulatory mechanisms of the cellular antiviral response in teleosts.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00189-8.

在哺乳动物中，线粒体融合蛋白2（MFN2）参与线粒体融合，并抑制病毒诱导的RIG-I样受体（RLR）信号通路。然而，人们对MFN2在非哺乳动物物种中的功能知之甚少。在本研究中，我们在大黄鱼（Larimichthys crocea）中克隆了一个MFN2同源物（LcMFN2）。系统发育分析表明，MFN2是在文昌鱼和脊椎动物分化后出现的。MFN2的蛋白质序列从鱼类到哺乳动物都是非常保守的。LcMFN2在不同水平检查的所有组织/器官中表达，并且其表达在poly（I:C）刺激下上调。LcMFN2的过表达抑制了MAVS诱导的I型干扰素（IFN）启动子激活和抗病毒基因表达。相反，敲低内源性LcMFN2增强了poly（I:C）诱导的I型IFN的产生。此外，LcMFN2增强了MAVS的K48连接的多泛素化，促进了其降解。此外，LcMFN2的过表达损害了细胞抗病毒反应，如在感染鲤鱼春季病毒血症病毒（SVCV）的细胞中病毒基因表达增加和更严重的细胞病变效应（CPE）所证明的。这些结果表明，LcMFN2通过降解MAVS抑制I型IFN反应，表明其在细胞抗病毒反应中的负调控作用。因此，我们的研究为硬骨鱼细胞抗病毒反应的调节机制提供了新的线索。补充信息：在线版本包含补充材料，请访问10.1007/s42995-023-00189-8。

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引用次数: 0

An improved blood hemorrhaging treatment using diatoms frustules, by alternating Ca and light levels in cultures. 一种改进的血液出血治疗方法，使用硅藻截头体，通过交替培养中的钙和光照水平。

IF 5.7 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Marine Life Science & Technology

Pub Date : 2023-08-18 eCollection Date: 2023-08-01 DOI: 10.1007/s42995-023-00180-3

Qinfeng Li, Zheng He, Hussein E Rozan, Chao Feng, Xiaojie Cheng, Xiguang Chen

Hemorrhage control requires hemostatic materials that are both effective and biocompatible. Among these, diatom biosilica (DBs) could significantly improve hemorrhage control, but it induces hemolysis (the hemolysis rate > 5%). Thus, the purpose of this study was to explore the influence of Ca²⁺ biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate. Here, CaCl₂ was added to the diatom medium under high light (cool white, fluorescent lamps, 67.5 µmol m^-2 s^-1), producing Ca-DBs-3 with a particle size of 40-50 μm and a Ca²⁺ content of Ca-DBs-3 obtained from the higher concentration CaCl₂ group (6.7 mmol L^-1) of 0.16%. The liquid absorption capacity of Ca-DBs-3 was 30.43 ± 0.57 times its dry weight; the in vitro clotting time was comparable to QuikClot^® zeolite; the hemostatic time and blood loss using the rat tail amputation model were 36.40 ± 2.52 s and 0.39 ± 0.12 g, which were 40.72% and 19.50% of QuikClot^® zeolite, respectively. Ca-DBs-3 showed no apparent toxicity to L929 cells (cell viability > 80%) and was non-hemolysis (the hemolysis rate < 2%). This study prepared Ca-DBs-3 with a rapid hemostatic effect and good biocompatibility, providing a path to develop diatom biosilica hemostatic materials.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00180-3.

出血控制需要既有效又具有生物相容性的止血材料。其中，硅藻生物二氧化硅（DBs）可以显著改善出血控制，但它会引起溶血（溶血率 > 5%）。因此，本研究的目的是探索Ca2+生物矿化对DBs的影响，以开发具有低溶血率的快速止血材料。在这里，在强光下（冷白色，荧光灯，67.5µmol m-2 s-1）将CaCl2添加到硅藻培养基中，产生粒径为40-50μm的Ca-DBs-3，从较高浓度的CaCl2组（6.7 mmol L-1）获得的Ca-DBs-3的Ca2+含量为0.16%。Ca-DBs-3-的液体吸收能力为30.43 ± 干重的0.57倍；体外凝结时间与QuikClot®沸石相当；大鼠断尾模型止血时间和失血量分别为36.40 ± 2.52s和0.39 ± 0.12克，分别为QuikClot®沸石的40.72%和19.50%。Ca-DBs-3对L929细胞无明显毒性（细胞活力 > 80%）且为非溶血性（溶血率补充信息：在线版本包含补充材料，请访问10.1007/s42995-023-00180-3。

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引用次数: 0