首页 > 最新文献

Marine Life Science & Technology最新文献

英文 中文
Colonization-competition dynamics of basal species shape food web complexity in island metacommunities. 基础物种的定居竞争动态塑造了岛屿元群落中食物网的复杂性。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-05-09 eCollection Date: 2023-05-01 DOI: 10.1007/s42995-023-00167-0
Guanming Guo, Fei Zhao, Ivan Nijs, Jinbao Liao

Exploring how food web complexity emerges and evolves in island ecosystems remains a major challenge in ecology. Food webs assembled from multiple islands are commonly recognized as highly complex trophic networks that are dynamic in both space and time. In the context of global climate change, it remains unclear whether food web complexity will decrease in a monotonic fashion when undergoing habitat destruction (e.g., the inundation of islands due to sea-level rise). Here, we develop a simple yet comprehensive patch-dynamic framework for complex food web metacommunities subject to the competition-colonization tradeoff between basal species. We found that oscillations in food web topological complexity (characterized by species diversity, mean food chain length and the degree of omnivory) emerge along the habitat destruction gradient. This outcome is robust to changing parameters or relaxing the assumption of a strict competitive hierarchy. Having oscillations in food web complexity indicates that small habitat changes could have disproportionate negative effects on species diversity, thus the success of conservation actions should be evaluated not only on changes in biodiversity, but also on system robustness to habitat alteration. Overall, this study provides a parsimonious mechanistic explanation for the emergence of food web complexity in island ecosystems, further enriching our understanding of metacommunity assembly.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00167-0.

探索岛屿生态系统中食物网的复杂性是如何出现和演变的,仍然是生态学的一大挑战。由多个岛屿组成的食物网通常被认为是高度复杂的营养网络,在空间和时间上都是动态的。在全球气候变化的背景下,尚不清楚在栖息地遭到破坏(例如,由于海平面上升导致岛屿被淹没)时,食物网的复杂性是否会以单调的方式降低。在这里,我们为复杂的食物网元群落开发了一个简单而全面的补丁动态框架,该框架受基础物种之间竞争-殖民化权衡的影响。我们发现,食物网拓扑复杂性(以物种多样性、平均食物链长度和杂食性程度为特征)的振荡沿着栖息地破坏梯度出现。这种结果对于改变参数或放松严格竞争等级的假设是稳健的。食物网复杂性的波动表明,微小的栖息地变化可能会对物种多样性产生不成比例的负面影响,因此,保护行动的成功不仅应评估生物多样性的变化,还应评估系统对栖息地变化的稳健性。总的来说,这项研究为岛屿生态系统中食物网复杂性的出现提供了一个简约的机制解释,进一步丰富了我们对元群落组装的理解。补充信息:在线版本包含补充材料,请访问10.1007/s42995-023-00167-0。
{"title":"Colonization-competition dynamics of basal species shape food web complexity in island metacommunities.","authors":"Guanming Guo,&nbsp;Fei Zhao,&nbsp;Ivan Nijs,&nbsp;Jinbao Liao","doi":"10.1007/s42995-023-00167-0","DOIUrl":"10.1007/s42995-023-00167-0","url":null,"abstract":"<p><p>Exploring how food web complexity emerges and evolves in island ecosystems remains a major challenge in ecology. Food webs assembled from multiple islands are commonly recognized as highly complex trophic networks that are dynamic in both space and time. In the context of global climate change, it remains unclear whether food web complexity will decrease in a monotonic fashion when undergoing habitat destruction (e.g., the inundation of islands due to sea-level rise). Here, we develop a simple yet comprehensive patch-dynamic framework for complex food web metacommunities subject to the competition-colonization tradeoff between basal species. We found that oscillations in food web topological complexity (characterized by species diversity, mean food chain length and the degree of omnivory) emerge along the habitat destruction gradient. This outcome is robust to changing parameters or relaxing the assumption of a strict competitive hierarchy. Having oscillations in food web complexity indicates that small habitat changes could have disproportionate negative effects on species diversity, thus the success of conservation actions should be evaluated not only on changes in biodiversity, but also on system robustness to habitat alteration. Overall, this study provides a parsimonious mechanistic explanation for the emergence of food web complexity in island ecosystems, further enriching our understanding of metacommunity assembly.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00167-0.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 2","pages":"169-177"},"PeriodicalIF":5.7,"publicationDate":"2023-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9637819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery of a natural small-molecule AMP-activated kinase activator that alleviates nonalcoholic steatohepatitis. 发现一种天然小分子AMP活化激酶激活剂,可缓解非酒精性脂肪性肝炎。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-04-30 eCollection Date: 2023-05-01 DOI: 10.1007/s42995-023-00168-z
Jin Chen, Li Xu, Xue-Qing Zhang, Xue Liu, Zi-Xuan Zhang, Qiu-Mei Zhu, Jian-Yu Liu, Muhammad Omer Iqbal, Ning Ding, Chang-Lun Shao, Mei-Yan Wei, Yu-Chao Gu

Non-alcoholic steatohepatitis (NASH) is a primary cause of cirrhosis and hepatocellular carcinoma. Unfortunately, there is no approved drug treatment for NASH. AMP-activated kinase (AMPK) is an important metabolic sensor and whole-body regulator. It has been proposed that AMPK activators could be used for treating metabolic diseases such as obesity, type 2 diabetes and NASH. In this study, we screened a marine natural compound library by monitoring AMPK activity and found a potent AMPK activator, candidusin A (CHNQD-0803). Further studies showed that CHNQD-0803 directly binds recombinant AMPK with a KD value of 4.728 × 10-8 M and activates AMPK at both molecular and intracellular levels. We then investigated the roles and mechanisms of CHNQD-0803 in PA-induced fat deposition, LPS-stimulated inflammation, TGF-β-induced fibrosis cell models and the MCD-induced mouse model of NASH. The results showed that CHNQD-0803 inhibited the expression of adipogenesis genes and reduced fat deposition, negatively regulated the NF-κB-TNFα inflammatory axis to suppress inflammation, and ameliorated liver injury and fibrosis. These data indicate that CHNQD-0803 as an AMPK activator is a novel potential therapeutic candidate for NASH treatment.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00168-z.

非酒精性脂肪性肝炎(NASH)是肝硬化和肝细胞癌的主要原因。不幸的是,目前还没有批准的治疗NASH的药物。AMPK是一种重要的代谢传感器和全身调节因子。有人提出AMPK激活剂可用于治疗代谢性疾病,如肥胖、2型糖尿病和NASH。在本研究中,我们通过监测AMPK活性筛选了一个海洋天然化合物库,并发现了一种强效的AMPK激活剂,念珠菌素a(CHNQD-0803)。进一步的研究表明,CHNQD-0803直接结合重组AMPK,KD值为4.728 × 10-8M,并在分子和细胞内水平上激活AMPK。然后,我们研究了CHNQD-0803在PA诱导的脂肪沉积、LPS刺激的炎症、TGF-β诱导的纤维化细胞模型和MCD诱导的NASH小鼠模型中的作用和机制。结果表明,CHNQD-0803抑制脂肪生成基因的表达并减少脂肪沉积,负调控NF-κB-TNFα炎症轴以抑制炎症,改善肝损伤和纤维化。这些数据表明,作为AMPK激活剂的CHNQD-0803是NASH治疗的新的潜在治疗候选物。补充信息:在线版本包含补充材料,请访问10.1007/s42995-023-00168-z。
{"title":"Discovery of a natural small-molecule AMP-activated kinase activator that alleviates nonalcoholic steatohepatitis.","authors":"Jin Chen,&nbsp;Li Xu,&nbsp;Xue-Qing Zhang,&nbsp;Xue Liu,&nbsp;Zi-Xuan Zhang,&nbsp;Qiu-Mei Zhu,&nbsp;Jian-Yu Liu,&nbsp;Muhammad Omer Iqbal,&nbsp;Ning Ding,&nbsp;Chang-Lun Shao,&nbsp;Mei-Yan Wei,&nbsp;Yu-Chao Gu","doi":"10.1007/s42995-023-00168-z","DOIUrl":"10.1007/s42995-023-00168-z","url":null,"abstract":"<p><p>Non-alcoholic steatohepatitis (NASH) is a primary cause of cirrhosis and hepatocellular carcinoma. Unfortunately, there is no approved drug treatment for NASH. AMP-activated kinase (AMPK) is an important metabolic sensor and whole-body regulator. It has been proposed that AMPK activators could be used for treating metabolic diseases such as obesity, type 2 diabetes and NASH. In this study, we screened a marine natural compound library by monitoring AMPK activity and found a potent AMPK activator, candidusin A (<b>CHNQD-0803</b>). Further studies showed that <b>CHNQD-0803</b> directly binds recombinant AMPK with a <i>K</i><sub>D</sub> value of 4.728 × 10<sup>-8</sup> M and activates AMPK at both molecular and intracellular levels. We then investigated the roles and mechanisms of <b>CHNQD-0803</b> in PA-induced fat deposition, LPS-stimulated inflammation, TGF-β-induced fibrosis cell models and the MCD-induced mouse model of NASH. The results showed that <b>CHNQD-0803</b> inhibited the expression of adipogenesis genes and reduced fat deposition, negatively regulated the NF-κB-TNFα inflammatory axis to suppress inflammation, and ameliorated liver injury and fibrosis. These data indicate that <b>CHNQD-0803</b> as an AMPK activator is a novel potential therapeutic candidate for NASH treatment.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00168-z.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 2","pages":"196-210"},"PeriodicalIF":5.7,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9637820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Phylotype resolved spatial variation and association patterns of planktonic Thaumarchaeota in eastern Chinese marginal seas. 系统发育类型解析了中国东部边缘海域浮游Thaumarchaeota的空间变异和组合模式。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-04-07 eCollection Date: 2023-05-01 DOI: 10.1007/s42995-023-00169-y
Jiwen Liu, Fuyan Huang, Jiao Liu, Xiaoyue Liu, Ruiyun Lin, Xiaosong Zhong, Brian Austin, Xiao-Hua Zhang

The majority of marine ammonia oxidizers belong to Thaumarchaeota, a phylum of Archaea, which is distributed throughout the water column. Marine surface waters contain distinct thaumarchaeotal phylotypes compared to the deeper ocean, but spatial dynamics of the surface-associated lineages are largely unsolved. This study of 120 seawater samples from the eastern Chinese marginal seas identified contrasting distribution and association patterns among thaumarchaeotal phylotypes across different dimensions. Horizontally, Nitrosopumilus-like and Nitrosopelagicus-like phylotypes dominated the surface water (3 m) of the Yellow Sea (YS) and East China Sea (ECS), respectively, along with increased abundance of total free-living Thaumarchaeota in ECS. Similar compositional changes were observed in the surface microlayer. The spatial heterogeneity of particle-attached Thaumarchaeota was less clear in surface microlayers than in surface waters. Vertically, the Nitrosopelagicus-like phylotype increased in abundance from surface to 90 m in ECS, which led to an increase in the proportion of Thaumarchaeota relative to total prokaryotes. This occurred mainly in the free-living fraction. These results indicate a clear size-fractionated niche partitioning, which is more pronounced at lower depths than in the surface water/surface microlayer. In addition, associations of Thaumarchaeota with other microbial taxa varied between phylotypes and size fractions. Our results show that a phylotype-resolved and size-fractionated spatial heterogeneity of the thaumarchaeotal community is present in surface oceanic waters and a vertical variation of the Nitrosopelagicus-like phylotype is present in shallow shelf waters.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00169-y.

大多数海洋氨氧化剂属于太古菌门,分布在整个水柱中。与更深的海洋相比,海洋表层水域包含不同的thaumarchaeotal门型,但表层相关谱系的空间动力学在很大程度上尚未解决。这项研究对来自中国东部边缘海的120个海水样本进行了研究,确定了不同维度上thaumarchaeotal门型之间的对比分布和关联模式。在水平方向上,类Nitrosopumilus和类Nitrosopelagicus门型分别主导了黄海(YS)和东海(ECS)的表层水(3m),同时ECS中总自由生活Thaumarchaeota的丰度也有所增加。在表面微层中观察到类似的组成变化。附着颗粒的Thaumarchaeota在表层微层中的空间异质性不如在表层水中清晰。垂直地,在ECS中,Nitrosopelagicus样门型的丰度从表面增加到90m,这导致Thaumarchaeota相对于原核生物总数的比例增加。这主要发生在自由生活部分。这些结果表明,与地表水/地表微层相比,在较低的深度下,生态位划分更为明显。此外,Thaumarchaeota与其他微生物类群的联系在门型和大小组分之间存在差异。我们的研究结果表明,在表层海水中存在一个由门型分解和大小划分的thaumarchaeotal群落空间异质性,而在浅陆架水域中存在一种Nitrosopelagicus样门型的垂直变化。补充信息:在线版本包含补充材料,请访问10.1007/s42995-023-00169-y。
{"title":"Phylotype resolved spatial variation and association patterns of planktonic <i>Thaumarchaeota</i> in eastern Chinese marginal seas.","authors":"Jiwen Liu,&nbsp;Fuyan Huang,&nbsp;Jiao Liu,&nbsp;Xiaoyue Liu,&nbsp;Ruiyun Lin,&nbsp;Xiaosong Zhong,&nbsp;Brian Austin,&nbsp;Xiao-Hua Zhang","doi":"10.1007/s42995-023-00169-y","DOIUrl":"10.1007/s42995-023-00169-y","url":null,"abstract":"<p><p>The majority of marine ammonia oxidizers belong to <i>Thaumarchaeota</i>, a phylum of Archaea, which is distributed throughout the water column. Marine surface waters contain distinct thaumarchaeotal phylotypes compared to the deeper ocean, but spatial dynamics of the surface-associated lineages are largely unsolved. This study of 120 seawater samples from the eastern Chinese marginal seas identified contrasting distribution and association patterns among thaumarchaeotal phylotypes across different dimensions. Horizontally, <i>Nitrosopumilus</i>-like and <i>Nitrosopelagicus</i>-like phylotypes dominated the surface water (3 m) of the Yellow Sea (YS) and East China Sea (ECS), respectively, along with increased abundance of total free-living <i>Thaumarchaeota</i> in ECS. Similar compositional changes were observed in the surface microlayer. The spatial heterogeneity of particle-attached <i>Thaumarchaeota</i> was less clear in surface microlayers than in surface waters. Vertically, the <i>Nitrosopelagicus</i>-like phylotype increased in abundance from surface to 90 m in ECS, which led to an increase in the proportion of <i>Thaumarchaeota</i> relative to total prokaryotes. This occurred mainly in the free-living fraction. These results indicate a clear size-fractionated niche partitioning, which is more pronounced at lower depths than in the surface water/surface microlayer. In addition, associations of <i>Thaumarchaeota</i> with other microbial taxa varied between phylotypes and size fractions. Our results show that a phylotype-resolved and size-fractionated spatial heterogeneity of the thaumarchaeotal community is present in surface oceanic waters and a vertical variation of the <i>Nitrosopelagicus</i>-like phylotype is present in shallow shelf waters.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00169-y.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 2","pages":"257-270"},"PeriodicalIF":5.7,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9637369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Population structure and genome-wide evolutionary signatures reveal putative climate-driven habitat change and local adaptation in the large yellow croaker. 种群结构和全基因组进化特征揭示了气候驱动的大黄鱼栖息地变化和局部适应。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-04-07 eCollection Date: 2023-05-01 DOI: 10.1007/s42995-023-00165-2
Baohua Chen, Yulin Bai, Jiaying Wang, Qiaozhen Ke, Zhixiong Zhou, Tao Zhou, Ying Pan, Renxie Wu, Xiongfei Wu, Weiqiang Zheng, Peng Xu

The large yellow croaker (Larimichthys crocea) is one of the most economically valuable marine fish in China and is a notable species in ecological studies owing to a serious collapse of wild germplasm in the past few decades. The stock division and species distribution, which have important implications for ecological protection, germplasm recovery, and fishery resource management, have been debated since the 1960s. However, it is still uncertain even how many stocks exist in this species. To address this, we evaluated the fine-scale genetic structure of large yellow croaker populations distributed along the eastern and southern Chinese coastline based on 7.64 million SNP markers. Compared with the widely accepted stock boundaries proposed in the 1960s, our results revealed that a climate-driven habitat change probably occurred between the Naozhou (Nanhai) Stock and the Ming-Yuedong (Mindong) Stock. The boundary between these two stocks might have shifted northwards from the Pearl River Estuary to the northern area of the Taiwan Strait, accompanied by highly asymmetric introgression. In addition, we found divergent landscapes of natural selection between the stocks inhabiting northern and southern areas. The northern population exhibited highly agminated signatures of strong natural selection in genes related to developmental processes, whereas moderate and interspersed selective signatures were detected in many immune-related genes in the southern populations. These findings establish the stock status and genome-wide evolutionary landscapes of large yellow croaker, providing a basis for conservation, fisheries management and further evolutionary biology studies.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00165-2.

大黄鱼(Larimichthys crocea)是中国最具经济价值的海洋鱼类之一,由于过去几十年野生种质资源的严重崩溃,它是生态学研究中的一个著名物种。种群划分和物种分布对生态保护、种质资源恢复和渔业资源管理具有重要意义,自20世纪60年代以来一直存在争议。然而,这个物种究竟有多少种群还不确定。为了解决这一问题,我们基于764万个SNP标记评估了分布在中国东部和南部海岸线的大黄鱼种群的精细遗传结构。与20世纪60年代提出的被广泛接受的种群边界相比,我们的研究结果表明,闹洲(南海)种群和明粤东(闽东)种群之间可能发生了气候驱动的栖息地变化。这两个种群之间的边界可能从珠江口向北转移到台湾海峡北部地区,并伴随着高度不对称的海进。此外,我们发现栖息在北部和南部地区的种群之间存在着不同的自然选择景观。北方种群在与发育过程相关的基因中表现出强烈的自然选择的高度分化特征,而南方种群的许多免疫相关基因中检测到中等和分散的选择性特征。这些发现确立了大黄鱼的种群状况和全基因组进化景观,为保护、渔业管理和进一步的进化生物学研究提供了基础。补充信息:在线版本包含补充材料,请访问10.1007/s42995-023-00165-2。
{"title":"Population structure and genome-wide evolutionary signatures reveal putative climate-driven habitat change and local adaptation in the large yellow croaker.","authors":"Baohua Chen,&nbsp;Yulin Bai,&nbsp;Jiaying Wang,&nbsp;Qiaozhen Ke,&nbsp;Zhixiong Zhou,&nbsp;Tao Zhou,&nbsp;Ying Pan,&nbsp;Renxie Wu,&nbsp;Xiongfei Wu,&nbsp;Weiqiang Zheng,&nbsp;Peng Xu","doi":"10.1007/s42995-023-00165-2","DOIUrl":"10.1007/s42995-023-00165-2","url":null,"abstract":"<p><p>The large yellow croaker (<i>Larimichthys</i> <i>crocea</i>) is one of the most economically valuable marine fish in China and is a notable species in ecological studies owing to a serious collapse of wild germplasm in the past few decades. The stock division and species distribution, which have important implications for ecological protection, germplasm recovery, and fishery resource management, have been debated since the 1960s. However, it is still uncertain even how many stocks exist in this species. To address this, we evaluated the fine-scale genetic structure of large yellow croaker populations distributed along the eastern and southern Chinese coastline based on 7.64 million SNP markers. Compared with the widely accepted stock boundaries proposed in the 1960s, our results revealed that a climate-driven habitat change probably occurred between the Naozhou (Nanhai) Stock and the Ming-Yuedong (Mindong) Stock. The boundary between these two stocks might have shifted northwards from the Pearl River Estuary to the northern area of the Taiwan Strait, accompanied by highly asymmetric introgression. In addition, we found divergent landscapes of natural selection between the stocks inhabiting northern and southern areas. The northern population exhibited highly agminated signatures of strong natural selection in genes related to developmental processes, whereas moderate and interspersed selective signatures were detected in many immune-related genes in the southern populations. These findings establish the stock status and genome-wide evolutionary landscapes of large yellow croaker, providing a basis for conservation, fisheries management and further evolutionary biology studies.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00165-2.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 2","pages":"141-154"},"PeriodicalIF":5.7,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9637818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyketides with potential bioactivities from the mangrove-derived fungus Talaromyces sp. WHUF0362. 红树林真菌Talaromyces sp.WHUF30362中具有潜在生物活性的聚酮。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-03-31 eCollection Date: 2023-05-01 DOI: 10.1007/s42995-023-00170-5
Huawei Lv, Haibo Su, Yaxin Xue, Jia Jia, Hongkai Bi, Shoubao Wang, Jinkun Zhang, Mengdi Zhu, Mahmoud Emam, Hong Wang, Kui Hong, Xing-Nuo Li

Metabolites of microorganisms have long been considered as potential sources for drug discovery. In this study, five new depsidone derivatives, talaronins A-E (1-5) and three new xanthone derivatives, talaronins F-H (6-8), together with 16 known compounds (9-24), were isolated from the ethyl acetate extract of the mangrove-derived fungus Talaromyces species WHUF0362. The structures were elucidated by analysis of spectroscopic data and chemical methods including alkaline hydrolysis and Mosher's method. Compounds 1 and 2 each attached a dimethyl acetal group at the aromatic ring. A putative biogenetic relationship of the isolated metabolites was presented and suggested that the depsidones and the xanthones probably had the same biosynthetic precursors such as chrysophanol or rheochrysidin. The antimicrobial activity assay indicated that compounds 5, 9, 10, and 14 showed potent activity against Helicobacter pylori with minimum inhibitory concentration (MIC) values in the range of 2.42-36.04 μmol/L. While secalonic acid D (19) demonstrated significant antimicrobial activity against four strains of H. pylori with MIC values in the range of 0.20 to 1.57 μmol/L. Furthermore, secalonic acid D (19) exhibited cytotoxicity against cancer cell lines Bel-7402 and HCT-116 with IC50 values of 0.15 and 0.19 μmol/L, respectively. The structure-activity relationship of depsidone derivatives revealed that the presence of the lactone ring and the hydroxyl at C-10 was crucial to the antimicrobial activity against H. pylori. The depsidone derivatives are promising leads to inhibit H. pylori and provide an avenue for further development of novel antibiotics.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00170-5.

微生物的代谢产物长期以来一直被认为是药物发现的潜在来源。在本研究中,从红树林真菌Talaromyces物种WHUF0362的乙酸乙酯提取物中分离出5种新的去糖苷酮衍生物talaronins A-E(1-5)和3种新的黄酮衍生物Talalonins F-H(6-8),以及16种已知化合物(9-24)。通过光谱数据分析和化学方法(包括碱水解法和Mosher法)阐明了它们的结构。化合物1和2各自在芳环上连接有二甲基缩醛基团。提出了分离代谢产物的假定生物成因关系,并表明去糖苷酮和黄酮可能具有相同的生物合成前体,如大黄酚或流变chrysidin。抗菌活性测定表明,化合物5、9、10和14对幽门螺杆菌表现出有效的抗菌活性,最小抑菌浓度(MIC)值在2.42-36.04μmol/L范围内。而丝卡菌酸D(19)对4株幽门螺杆菌显示出显著的抗菌活性。MIC值在0.20-1.57μmol/L范围。此外,secalonic acid D(19)对癌症细胞株Bel-7402和HCT-116具有细胞毒性,IC50分别为0.15和0.19μmol/L。去糖苷酮衍生物的结构-活性关系表明,C-10处内酯环和羟基的存在对抗幽门螺杆菌的抗菌活性至关重要。去糖苷酮衍生物有望抑制幽门螺杆菌,为进一步开发新型抗生素提供了途径。补充信息:在线版本包含补充材料,请访问10.1007/s42995-023-00170-5。
{"title":"Polyketides with potential bioactivities from the mangrove-derived fungus <i>Talaromyces</i> sp. WHUF0362.","authors":"Huawei Lv,&nbsp;Haibo Su,&nbsp;Yaxin Xue,&nbsp;Jia Jia,&nbsp;Hongkai Bi,&nbsp;Shoubao Wang,&nbsp;Jinkun Zhang,&nbsp;Mengdi Zhu,&nbsp;Mahmoud Emam,&nbsp;Hong Wang,&nbsp;Kui Hong,&nbsp;Xing-Nuo Li","doi":"10.1007/s42995-023-00170-5","DOIUrl":"10.1007/s42995-023-00170-5","url":null,"abstract":"<p><p>Metabolites of microorganisms have long been considered as potential sources for drug discovery. In this study, five new depsidone derivatives, talaronins A-E (<b>1-5</b>) and three new xanthone derivatives, talaronins F-H (<b>6-8</b>), together with 16 known compounds (<b>9-24</b>), were isolated from the ethyl acetate extract of the mangrove-derived fungus <i>Talaromyces</i> species WHUF0362. The structures were elucidated by analysis of spectroscopic data and chemical methods including alkaline hydrolysis and Mosher's method. Compounds <b>1</b> and <b>2</b> each attached a dimethyl acetal group at the aromatic ring. A putative biogenetic relationship of the isolated metabolites was presented and suggested that the depsidones and the xanthones probably had the same biosynthetic precursors such as chrysophanol or rheochrysidin. The antimicrobial activity assay indicated that compounds <b>5</b>, <b>9</b>, <b>10</b>, and <b>14</b> showed potent activity against <i>Helicobacter pylori</i> with minimum inhibitory concentration (MIC) values in the range of 2.42-36.04 μmol/L. While secalonic acid D (<b>19</b>) demonstrated significant antimicrobial activity against four strains of <i>H. pylori</i> with MIC values in the range of 0.20 to 1.57 μmol/L. Furthermore, secalonic acid D (<b>19</b>) exhibited cytotoxicity against cancer cell lines Bel-7402 and HCT-116 with IC<sub>50</sub> values of 0.15 and 0.19 μmol/L, respectively. The structure-activity relationship of depsidone derivatives revealed that the presence of the lactone ring and the hydroxyl at C-10 was crucial to the antimicrobial activity against <i>H. pylori</i>. The depsidone derivatives are promising leads to inhibit <i>H. pylori</i> and provide an avenue for further development of novel antibiotics.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00170-5.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 2","pages":"232-241"},"PeriodicalIF":5.7,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9584950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Genomic characterization and molecular dating of the novel bacterium Permianibacter aggregans HW001T, which originated from Permian ground water. 源于二叠纪地下水的新型细菌 Permianibacter aggregans HW001T 的基因组特征和分子年代测定。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-02-24 eCollection Date: 2023-02-01 DOI: 10.1007/s42995-023-00164-3
Shuangfei Zhang, Russell T Hill, Hui Wang

The Permian Basin is a unique ecosystem located in the southwest of the USA. An unanswered question is whether the bacteria in the Permian Basin adapted to the changing paleomarine environment and survived in the remnants of Permian groundwater. In our previous study, a novel bacterial strain, Permianibacter aggregans HW001T, was isolated from microalgae cultures incubated with Permian Basin waters, and was shown to originate from the Permian Ocean. In this study, strain HW001T was shown to be the representative strain of a novel family, classified as 'Permianibacteraceae'. The results of molecular dating suggested that the strain HW001T diverged ~ 447 million years ago (mya), which is the early Permian period (~ 250 mya). Genome analysis was used to access its potential energy utilization and biosynthesis capacity. A large number of transporters, carbohydrate-active enzymes and protein-degradation related genes have been annotated in the genome of strain HW001T. In addition, a series of important metabolic pathways, such as peptidoglycan biosynthesis, osmotic stress response system and multifunctional quorum sensing were annotated, which may confer the ability to adapt to various unfavorable environmental conditions. Finally, the evolutionary history of strain HW001T was reconstructed and the horizontal transfer of genes was predicted, indicating that the adaptation of P. aggregans to a changing marine environment depends on the evolution of their metabolic capabilities, especially in signal transmission. In conclusion, the results of this study provide genomic information for revealing the adaptive mechanism of strain HW001T to the changing ancient oceans.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-023-00164-3.

二叠纪盆地位于美国西南部,是一个独特的生态系统。一个悬而未决的问题是,二叠纪盆地的细菌是否适应了不断变化的古海洋环境,并在二叠纪地下水遗迹中生存下来。在我们之前的研究中,从用二叠纪盆地水培养的微藻中分离出了一株新的细菌,即 Permianibacter aggregans HW001T,并证明它来自二叠纪海洋。在这项研究中,菌株 HW001T 被证明是一个新家族的代表菌株,被归类为 "二叠纪细菌科"。分子年代测定结果表明,HW001T 菌株分化于距今约 4.47 亿年前,即二叠纪早期(约 2.5 亿年前)。基因组分析用于了解其潜在的能量利用和生物合成能力。在菌株 HW001T 的基因组中注释了大量转运体、碳水化合物活性酶和蛋白质降解相关基因。此外,还注释了一系列重要的代谢途径,如肽聚糖生物合成、渗透胁迫响应系统和多功能法定量感应等,这可能赋予了菌株适应各种不利环境条件的能力。最后,重建了菌株 HW001T 的进化历史,并预测了基因的水平转移,这表明聚合细菌对不断变化的海洋环境的适应取决于其代谢能力的进化,尤其是在信号传递方面。总之,本研究结果为揭示菌株 HW001T 对不断变化的古代海洋的适应机制提供了基因组信息:在线版本包含补充材料,可查阅 10.1007/s42995-023-00164-3。
{"title":"Genomic characterization and molecular dating of the novel bacterium <i>Permianibacter aggregans</i> HW001<sup>T</sup>, which originated from Permian ground water.","authors":"Shuangfei Zhang, Russell T Hill, Hui Wang","doi":"10.1007/s42995-023-00164-3","DOIUrl":"10.1007/s42995-023-00164-3","url":null,"abstract":"<p><p>The Permian Basin is a unique ecosystem located in the southwest of the USA. An unanswered question is whether the bacteria in the Permian Basin adapted to the changing paleomarine environment and survived in the remnants of Permian groundwater. In our previous study, a novel bacterial strain, <i>Permianibacter aggregans</i> HW001<sup>T</sup>, was isolated from microalgae cultures incubated with Permian Basin waters, and was shown to originate from the Permian Ocean. In this study, strain HW001<sup>T</sup> was shown to be the representative strain of a novel family, classified as 'Permianibacteraceae'. The results of molecular dating suggested that the strain HW001<sup>T</sup> diverged ~ 447 million years ago (mya), which is the early Permian period (~ 250 mya). Genome analysis was used to access its potential energy utilization and biosynthesis capacity. A large number of transporters, carbohydrate-active enzymes and protein-degradation related genes have been annotated in the genome of strain HW001<sup>T</sup>. In addition, a series of important metabolic pathways, such as peptidoglycan biosynthesis, osmotic stress response system and multifunctional quorum sensing were annotated, which may confer the ability to adapt to various unfavorable environmental conditions. Finally, the evolutionary history of strain HW001<sup>T</sup> was reconstructed and the horizontal transfer of genes was predicted, indicating that the adaptation of <i>P. aggregans</i> to a changing marine environment depends on the evolution of their metabolic capabilities, especially in signal transmission. In conclusion, the results of this study provide genomic information for revealing the adaptive mechanism of strain HW001<sup>T</sup> to the changing ancient oceans.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-023-00164-3.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 1","pages":"12-27"},"PeriodicalIF":5.7,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9440770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution of sex determination in crustaceans. 甲壳动物性别决定的进化。
IF 5.8 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-02-22 eCollection Date: 2023-02-01 DOI: 10.1007/s42995-023-00163-4
Zhiqiang Ye, Trent Bishop, Yaohai Wang, Ryan Shahriari, Michael Lynch

Sex determination (SD) involves mechanisms that determine whether an individual will develop into a male, female, or in rare cases, hermaphrodite. Crustaceans harbor extremely diverse SD systems, including hermaphroditism, environmental sex determination (ESD), genetic sex determination (GSD), and cytoplasmic sex determination (e.g., Wolbachia controlled SD systems). Such diversity lays the groundwork for researching the evolution of SD in crustaceans, i.e., transitions among different SD systems. However, most previous research has focused on understanding the mechanism of SD within a single lineage or species, overlooking the transition across different SD systems. To help bridge this gap, we summarize the understanding of SD in various clades of crustaceans, and discuss how different SD systems might evolve from one another. Furthermore, we review the genetic basis for transitions between different SD systems (i.e., Dmrt genes) and propose the microcrustacean Daphnia (clade Branchiopoda) as a model to study the transition from ESD to GSD.

性别决定(SD)涉及决定个体是否发育成雄性、雌性或在极少数情况下雌雄同体的机制。甲壳类动物的性别决定系统极其多样化,包括雌雄同体、环境性别决定(ESD)、遗传性别决定(GSD)和细胞质性别决定(例如沃尔巴克氏体控制的性别决定系统)。这种多样性为研究甲壳动物的性别决定进化(即不同性别决定系统之间的过渡)奠定了基础。然而,以往的研究大多集中于了解单一品系或物种内的 SD 机制,忽略了不同 SD 系统之间的过渡。为了弥补这一差距,我们总结了甲壳类各支系对SD的理解,并讨论了不同SD系统可能如何相互演化。此外,我们还回顾了不同SD系统(即Dmrt基因)之间过渡的遗传基础,并建议以微型甲壳动物水蚤(Branchiopoda科)为模型,研究从ESD到GSD的过渡。
{"title":"Evolution of sex determination in crustaceans.","authors":"Zhiqiang Ye, Trent Bishop, Yaohai Wang, Ryan Shahriari, Michael Lynch","doi":"10.1007/s42995-023-00163-4","DOIUrl":"10.1007/s42995-023-00163-4","url":null,"abstract":"<p><p>Sex determination (SD) involves mechanisms that determine whether an individual will develop into a male, female, or in rare cases, hermaphrodite. Crustaceans harbor extremely diverse SD systems, including hermaphroditism, environmental sex determination (ESD), genetic sex determination (GSD), and cytoplasmic sex determination (e.g., <i>Wolbachia</i> controlled SD systems). Such diversity lays the groundwork for researching the evolution of SD in crustaceans, i.e., transitions among different SD systems. However, most previous research has focused on understanding the mechanism of SD within a single lineage or species, overlooking the transition across different SD systems. To help bridge this gap, we summarize the understanding of SD in various clades of crustaceans, and discuss how different SD systems might evolve from one another. Furthermore, we review the genetic basis for transitions between different SD systems (i.e., <i>Dmrt</i> genes) and propose the microcrustacean <i>Daphnia</i> (clade Branchiopoda) as a model to study the transition from ESD to GSD.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 1","pages":"1-11"},"PeriodicalIF":5.8,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Early life history affects fish size mainly by indirectly regulating the growth during each stage: a case study in a demersal fish. 早期生活史主要通过间接调节每个阶段的生长来影响鱼体大小:底栖鱼类案例研究。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-02-19 eCollection Date: 2023-02-01 DOI: 10.1007/s42995-022-00145-y
Rui Wu, Qinghuan Zhu, Satoshi Katayama, Yongjun Tian, Jianchao Li, Kunihiro Fujiwara, Yoji Narimatsu

The complex life histories of demersal fishes are artificially separated into multiple stages along with changes in morphology and habitat. It is worth exploring whether the phenotypes expressed earlier and later during the life cycle are related or decoupled. The life stages of first year Pacific cod (Gadus macrocephalus) were tracked over different hatch years and regions to test whether the early life history had a long-lasting effect on subsequent growth. We further explored the contribution of growth in the early and subsequent life history stages to body size at the end of each stage. In addition to the accessory growth centre and the first annual ring, the other two checks on the otolith possibly related to settlement and entering deeper waters were identified in 75 Pacific cod individuals. The direct and indirect relationships among the life history stages was interpreted based on path analysis. The results showed that growth prior to the formation of the accessory growth centre had a significant effect on the absolute growth of the fish before and after settlement and migration to deep water. However, there was no or moderate evidence that early growth affected the body size at each stage, which was mainly regulated by growth during the stage. This study supports the lasting effect of early growth and clarifies that it affects size mainly by indirectly regulating staged growth. Quantifying the phenotype relationships and identifying the internal mechanisms form the basis for assessing population dynamics and understanding the processes behind the changes.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-022-00145-y.

底栖鱼类复杂的生活史随着形态和栖息地的变化被人为地分为多个阶段。值得探讨的是,生命周期早期和晚期表现出的表型是相关的还是脱钩的。我们在不同的孵化年份和地区对太平洋鳕鱼(Gadus macrocephalus)第一年的生命阶段进行了追踪,以检验早期的生命史是否会对随后的生长产生长期影响。我们进一步探讨了早期和后续生活史阶段的生长对每个阶段结束时体型的贡献。除了附属生长中心和第一个年轮外,我们还在 75 个太平洋鳕鱼个体的耳石上发现了可能与定居和进入深海有关的另外两个检查点。根据路径分析解释了各生活史阶段之间的直接和间接关系。结果表明,附属生长中心形成之前的生长对鱼类定居和洄游到深水前后的绝对生长有显著影响。然而,没有证据或中等程度的证据表明早期生长会影响各阶段的体型,体型主要受该阶段的生长调节。这项研究支持早期生长的持久效应,并阐明早期生长主要通过间接调节阶段性生长来影响体型。量化表型关系和确定内部机制是评估种群动态和了解变化背后过程的基础:在线版本包含补充材料,可查阅 10.1007/s42995-022-00145-y。
{"title":"Early life history affects fish size mainly by indirectly regulating the growth during each stage: a case study in a demersal fish.","authors":"Rui Wu, Qinghuan Zhu, Satoshi Katayama, Yongjun Tian, Jianchao Li, Kunihiro Fujiwara, Yoji Narimatsu","doi":"10.1007/s42995-022-00145-y","DOIUrl":"10.1007/s42995-022-00145-y","url":null,"abstract":"<p><p>The complex life histories of demersal fishes are artificially separated into multiple stages along with changes in morphology and habitat. It is worth exploring whether the phenotypes expressed earlier and later during the life cycle are related or decoupled. The life stages of first year Pacific cod (<i>Gadus macrocephalus</i>) were tracked over different hatch years and regions to test whether the early life history had a long-lasting effect on subsequent growth. We further explored the contribution of growth in the early and subsequent life history stages to body size at the end of each stage. In addition to the accessory growth centre and the first annual ring, the other two checks on the otolith possibly related to settlement and entering deeper waters were identified in 75 Pacific cod individuals. The direct and indirect relationships among the life history stages was interpreted based on path analysis. The results showed that growth prior to the formation of the accessory growth centre had a significant effect on the absolute growth of the fish before and after settlement and migration to deep water. However, there was no or moderate evidence that early growth affected the body size at each stage, which was mainly regulated by growth during the stage. This study supports the lasting effect of early growth and clarifies that it affects size mainly by indirectly regulating staged growth. Quantifying the phenotype relationships and identifying the internal mechanisms form the basis for assessing population dynamics and understanding the processes behind the changes.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-022-00145-y.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 1","pages":"75-84"},"PeriodicalIF":5.7,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9752857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of marine natural product Pretrichodermamide B as a STAT3 inhibitor for efficient anticancer therapy. 鉴定海洋天然产物 Pretrichodermamide B 作为 STAT3 抑制剂用于高效抗癌治疗。
IF 5.8 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-02-06 eCollection Date: 2023-02-01 DOI: 10.1007/s42995-022-00162-x
Rui Li, Yue Zhou, Xinxin Zhang, Lujia Yang, Jieyu Liu, Samantha M Wightman, Ling Lv, Zhiqing Liu, Chang-Yun Wang, Chenyang Zhao

The Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) regulates the expression of various critical mediators of cancer and is considered as one of the central communication nodes in cell growth and survival. Marine natural products (MNP) represent great resources for discovery of bioactive lead compounds, especially anti-cancer agents. Through the medium-throughput screening of our in-house MNP library, Pretrichodermamide B, an epidithiodiketopiperazine, was identified as a JAK/STAT3 signaling inhibitor. Further studies identified that Pretrichodermamide B directly binds to STAT3, preventing phosphorylation and thus inhibiting JAK/STAT3 signaling. Moreover, it suppressed cancer cell growth, in vitro, at low micromolar concentrations and demonstrated efficacy in vivo by decreasing tumor growth in a xenograft mouse model. In addition, it was shown that Pretrichodermamide B was able to induce cell cycle arrest and promote cell apoptosis. This study demonstrated that Pretrichodermamide B is a novel STAT3 inhibitor, which should be considered for further exploration as a promising anti-cancer therapy.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-022-00162-x.

Janus 激酶(JAK)/信号转导和激活转录 3(STAT3)调节各种癌症关键介质的表达,被认为是细胞生长和存活的核心通讯节点之一。海洋天然产物(MNP)是发现生物活性先导化合物(尤其是抗癌剂)的重要资源。通过对我们内部的 MNP 库进行中等通量筛选,Pretrichodermamide B(一种表二硫二酮哌嗪)被鉴定为 JAK/STAT3 信号抑制剂。进一步研究发现,Pretrichodermamide B 可直接与 STAT3 结合,阻止其磷酸化,从而抑制 JAK/STAT3 信号转导。此外,它还能在体外低微摩尔浓度下抑制癌细胞的生长,并通过降低异种移植小鼠模型中肿瘤的生长而显示出体内疗效。此外,研究还表明 Pretrichodermamide B 能够诱导细胞周期停滞并促进细胞凋亡。这项研究表明,Pretrichodermamide B 是一种新型 STAT3 抑制剂,应考虑将其作为一种有前景的抗癌疗法进行进一步探索:在线版本包含补充材料,可查阅 10.1007/s42995-022-00162-x。
{"title":"Identification of marine natural product Pretrichodermamide B as a STAT3 inhibitor for efficient anticancer therapy.","authors":"Rui Li, Yue Zhou, Xinxin Zhang, Lujia Yang, Jieyu Liu, Samantha M Wightman, Ling Lv, Zhiqing Liu, Chang-Yun Wang, Chenyang Zhao","doi":"10.1007/s42995-022-00162-x","DOIUrl":"10.1007/s42995-022-00162-x","url":null,"abstract":"<p><p>The Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) regulates the expression of various critical mediators of cancer and is considered as one of the central communication nodes in cell growth and survival. Marine natural products (MNP) represent great resources for discovery of bioactive lead compounds, especially anti-cancer agents. Through the medium-throughput screening of our in-house MNP library, Pretrichodermamide B, an epidithiodiketopiperazine, was identified as a JAK/STAT3 signaling inhibitor. Further studies identified that Pretrichodermamide B directly binds to STAT3, preventing phosphorylation and thus inhibiting JAK/STAT3 signaling. Moreover, it suppressed cancer cell growth, in vitro, at low micromolar concentrations and demonstrated efficacy in vivo by decreasing tumor growth in a xenograft mouse model. In addition, it was shown that Pretrichodermamide B was able to induce cell cycle arrest and promote cell apoptosis. This study demonstrated that Pretrichodermamide B is a novel STAT3 inhibitor, which should be considered for further exploration as a promising anti-cancer therapy.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-022-00162-x.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 1","pages":"94-101"},"PeriodicalIF":5.8,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High cholesterol intake remodels cholesterol turnover and energy homeostasis in Nile tilapia (Oreochromis niloticus). 高胆固醇摄入重塑尼罗罗非鱼(Oreochromis niloticus)胆固醇转换和能量稳态。
IF 5.7 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Pub Date : 2023-02-01 DOI: 10.1007/s42995-022-00158-7
Rui-Xin Li, Ling-Yun Chen, Samwel M Limbu, Yu-Cheng Qian, Wen-Hao Zhou, Li-Qiao Chen, Yuan Luo, Fang Qiao, Mei-Ling Zhang, Zhen-Yu Du

The roles of dietary cholesterol in fish physiology are currently contradictory. The issue reflects the limited studies on the metabolic consequences of cholesterol intake in fish. The present study investigated the metabolic responses to high cholesterol intake in Nile tilapia (Oreochromis niloticus), which were fed with four cholesterol-contained diets (0.8, 1.6, 2.4 and 3.2%) and a control diet for eight weeks. All fish-fed cholesterol diets showed increased body weight, but accumulated cholesterol (the peak level was in the 1.6% cholesterol group). Then, we selected 1.6% cholesterol and control diets for further analysis. The high cholesterol diet impaired liver function and reduced mitochondria number in fish. Furthermore, high cholesterol intake triggered protective adaptation via (1) inhibiting endogenous cholesterol synthesis, (2) elevating the expression of genes related to cholesterol esterification and efflux, and (3) promoting chenodeoxycholic acid synthesis and efflux. Accordingly, high cholesterol intake reshaped the fish gut microbiome by increasing the abundance of Lactobacillus spp. and Mycobacterium spp., both of which are involved in cholesterol and/or bile acids catabolism. Moreover, high cholesterol intake inhibited lipid catabolic activities through mitochondrial β-oxidation, and lysosome-mediated lipophagy, and depressed insulin signaling sensitivity. Protein catabolism was elevated as a compulsory response to maintain energy homeostasis. Therefore, although high cholesterol intake promoted growth, it led to metabolic disorders in fish. For the first time, this study provides evidence for the systemic metabolic response to high cholesterol intake in fish. This knowledge contributes to an understanding of the metabolic syndromes caused by high cholesterol intake or deposition in fish.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-022-00158-7.

膳食胆固醇在鱼类生理中的作用目前是矛盾的。这个问题反映了对鱼类摄入胆固醇的代谢后果的有限研究。本研究研究了尼罗罗非鱼(Oreochromis niloticus)对高胆固醇摄入的代谢反应,分别饲喂4种含胆固醇饲料(0.8、1.6、2.4和3.2%)和对照饲料8周。所有鱼饲胆固醇饲料均显示体重增加,但胆固醇积累(峰值水平在1.6%胆固醇组)。然后,我们选择1.6%胆固醇和对照饮食进行进一步分析。高胆固醇饮食损害了鱼类的肝功能并减少了线粒体数量。此外,高胆固醇摄入通过以下途径触发保护性适应:(1)抑制内源性胆固醇合成,(2)提高胆固醇酯化和外排相关基因的表达,以及(3)促进鹅去氧胆酸合成和外排。因此,高胆固醇摄入通过增加乳酸杆菌和分枝杆菌的丰度重塑了鱼类肠道微生物群,这两种细菌都参与胆固醇和/或胆汁酸的分解代谢。此外,高胆固醇摄入通过线粒体β氧化和溶酶体介导的脂质吞噬抑制脂质分解代谢活动,并降低胰岛素信号敏感性。蛋白质分解代谢作为维持能量稳态的强制性反应而升高。因此,虽然高胆固醇摄入促进了鱼的生长,但它会导致鱼的代谢紊乱。本研究首次为鱼类对高胆固醇摄入的全身代谢反应提供了证据。这一知识有助于理解高胆固醇摄入或沉积在鱼类体内引起的代谢综合征。补充资料:在线版本包含补充资料,下载地址:10.1007/s42995-022-00158-7。
{"title":"High cholesterol intake remodels cholesterol turnover and energy homeostasis in Nile tilapia (<i>Oreochromis niloticus</i>).","authors":"Rui-Xin Li,&nbsp;Ling-Yun Chen,&nbsp;Samwel M Limbu,&nbsp;Yu-Cheng Qian,&nbsp;Wen-Hao Zhou,&nbsp;Li-Qiao Chen,&nbsp;Yuan Luo,&nbsp;Fang Qiao,&nbsp;Mei-Ling Zhang,&nbsp;Zhen-Yu Du","doi":"10.1007/s42995-022-00158-7","DOIUrl":"https://doi.org/10.1007/s42995-022-00158-7","url":null,"abstract":"<p><p>The roles of dietary cholesterol in fish physiology are currently contradictory. The issue reflects the limited studies on the metabolic consequences of cholesterol intake in fish. The present study investigated the metabolic responses to high cholesterol intake in Nile tilapia (<i>Oreochromis niloticus</i>), which were fed with four cholesterol-contained diets (0.8, 1.6, 2.4 and 3.2%) and a control diet for eight weeks. All fish-fed cholesterol diets showed increased body weight, but accumulated cholesterol (the peak level was in the 1.6% cholesterol group). Then, we selected 1.6% cholesterol and control diets for further analysis. The high cholesterol diet impaired liver function and reduced mitochondria number in fish. Furthermore, high cholesterol intake triggered protective adaptation via (1) inhibiting endogenous cholesterol synthesis, (2) elevating the expression of genes related to cholesterol esterification and efflux, and (3) promoting chenodeoxycholic acid synthesis and efflux. Accordingly, high cholesterol intake reshaped the fish gut microbiome by increasing the abundance of <i>Lactobacillus</i> spp. and <i>Mycobacterium</i> spp., both of which are involved in cholesterol and/or bile acids catabolism. Moreover, high cholesterol intake inhibited lipid catabolic activities through mitochondrial β-oxidation, and lysosome-mediated lipophagy, and depressed insulin signaling sensitivity. Protein catabolism was elevated as a compulsory response to maintain energy homeostasis. Therefore, although high cholesterol intake promoted growth, it led to metabolic disorders in fish. For the first time, this study provides evidence for the systemic metabolic response to high cholesterol intake in fish. This knowledge contributes to an understanding of the metabolic syndromes caused by high cholesterol intake or deposition in fish.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42995-022-00158-7.</p>","PeriodicalId":53218,"journal":{"name":"Marine Life Science & Technology","volume":"5 1","pages":"56-74"},"PeriodicalIF":5.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
Marine Life Science & Technology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1