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Determination of sweeteners in wine by liquid chromatography coupled with mass spectrometry (LC/MS) 液相色谱-质谱联用法测定葡萄酒中的甜味剂
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2021-01-01 DOI: 10.1051/CTV/CTV2021360132
Nuno Neves, Natália Ribeiro, C. Esteves, P. Barros
Sweeteners are food additive substances that give a sweet taste to foods but their use in oenological practices is forbidden. Making use of the capabilities of liquid chromatography coupled with mass spectrometry, a method for wine analysis was developed and validated for the detection and quantitation of some of the most widely used sweeteners: aspartame, potassium acesulfame, sodium cyclamate, saccharin, sucralose and stevioside. A matrix-matched calibration was used for all compounds obtaining a linear concentration range from 50 μg/L to 1000 μg/L. The limit of detection ranged from 0.002 mg/L to 0.014 mg/L, and the limit of quantification varied between 0.005 mg/L and 0.048 mg/L. Precision and recovery were assessed for 50 μg/L, 250 μg/L and 1000 μg/L with repeatability and intermediate precision values from 0.6% to 21.6% and 2.7% to 26.4% respectively, and recoveries ranging from 60% to 126%. These results were achieved using minimal sample preparation with a fast and high throughput method that is applicable to a wide range of wine matrices.
甜味剂是一种食品添加剂,可以给食物带来甜味,但在酿酒实践中是禁止使用的。利用液相色谱与质谱联用的能力,开发了一种葡萄酒分析方法,并验证了对一些最广泛使用的甜味剂的检测和定量:阿斯巴甜、乙酰磺酸钾、甜蜜素钠、糖精、三氯蔗糖和甜菊糖甙。所有化合物均采用基质匹配校准,获得50 ~ 1000 μg/L的线性浓度范围。检出限为0.002 ~ 0.014 mg/L,定量限为0.005 ~ 0.048 mg/L。精密度和加样回收率分别为50、250和1000 μg/L,重复性和中间精密度分别为0.6% ~ 21.6%和2.7% ~ 26.4%,加样回收率为60% ~ 126%。这些结果是实现使用最小的样品制备与快速和高通量的方法,适用于广泛的葡萄酒基质。
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引用次数: 2
Native yeast from distinct organs of grapevines established in Queretaro, Mexico, and their potential oenological utilization 来自墨西哥克雷塔罗葡萄不同器官的原生酵母及其潜在的酿酒利用
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203501030
Yamile M. Barragán-Castillo, D. E. Miranda-Castilleja, J. A. Aldrete-Tapia, S. Arvizu-Medrano, R. Martínez-Peniche
The aim of this study was to isolate, identify and determine the oenological potential of yeasts present in Vitis vinifera organs of grapevines established in Queretaro State, Mexico. The yeast distribution was influenced by the organ and the sampling season, and the yeast populations ranged from 0.8 and 5.5 Log CFU/g. A total of 93 yeasts were isolated, identified by RFLP and confirmed by sequencing of the ITS region, prevailing Aureobasidium cf. melanogenum and Basidiomycota yeast. The identified species with previously reported oenological potential were: Pichia cf. kluyveri and Clavispora cf.opuntiae. Remarkably, P. cf. kluyveri 3.1HM showed killer phenotype and was the most tolerant to sulfur dioxide, and survived 72 h after its inoculation in ‘Tempranillo’ must. C. cf. opuntiae 5.7HM showed β-glucosidase activity, the highest tolerance to 5 % ethanol and 25 °Brix (sugar levels). On the contrary, Rhodotorula isolates were not tolerant to stress conditions, and R. mucilaginosa 8HM did not grow under must conditions. Mixed fermentation using H. uvarum NB108/S. cerevisiae N05 resulted in the highest volatile acidity (0.45 g/L acetic acid), while no differences for total acidity, alcohol strength, residual sugars and total SO2 were found between the mixed fermentations treatments. This study provides an insight into the yeast diversity present in grapevines established in Queretaro, Mexico, and the oenological potential of. P. cf. kluyveri 3.1HM.
本研究的目的是分离、鉴定和确定在墨西哥克雷塔罗州葡萄的葡萄器官中存在的酵母的酿酒潜力。酵母菌的分布受器官和采样季节的影响,酵母菌群在0.8 ~ 5.5 Log CFU/g之间。共分离到93株酵母,经RFLP和ITS区测序鉴定,主要为黑素金黄色葡萄球菌(auobasidium cf. melanogenum)和担子菌(Basidiomycota yeast)。已鉴定的有酿酒潜力的种有:毕赤酵母(Pichia cf. kluyveri)和克拉维斯波尔(Clavispora cf.opuntiae)。值得注意的是,p.c.f kluyveri 3.1HM表现出杀伤表型,对二氧化硫的耐受性最强,接种后在‘Tempranillo’must中存活72 h。C. C. C. opuntiae 5.7HM表现出β-葡萄糖苷酶活性,对5%乙醇和25°白度(糖水平)的耐受性最高。相反,红酵母菌株对胁迫条件不具有耐受性,黏胶红霉菌8HM在一定条件下不能生长。利用微孢杆菌NB108/S混合发酵。酵母N05的挥发性酸度最高(0.45 g/L乙酸),而总酸度、酒精浓度、残糖和总SO2在混合发酵处理之间没有差异。本研究提供了一个深入了解酵母多样性存在于葡萄藤建立在克雷塔罗,墨西哥,和酿酒潜力。p.c.k luyveri 3.1HM。
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引用次数: 3
Pathogen-related specificity of subtilase VVISBT4.19 X1 in the Vitis vinifera defence response 枯草酶VVISBT4.19 X1在葡萄防御反应中的病原相关特异性
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203501042
J. Figueiredo, J. Cunha, J. Eiras-Dias, M. S. Silva, A. Figueiredo
Grapevine, Vitis vinifera L., is one of the most cultivated fruit plants worldwide with high economic value. Powdery mildew and gray mold diseases, caused by Erysiphe necator and Botrytis cinerea, respectively, are within the most devastating diseases, which are controlled by using several fungicide applications over a single growing season. A more sustainable and environmentally friendly alternative for pest control is associated to the development of breeding programs, in which American and Asian Vitis species, presenting natural resistance characteristics, are crossed with V. vinifera varieties that are susceptible to diseases caused by fungal or oomycete pathogens. As a result, new grapevine varieties that combine the good berry quality with a high degree of resistance to grapevine pathogens are obtained. One example is the Vitis vinifera cv ‘Regent’ that acquired high tolerance degree against E. necator and Plasmopara viticola. To ensure durable resistance introgression in breeding programs, a full understanding of grapevine defence mechanisms is crucial. Previous studies on grapevine-P. viticola pathosystem have suggested the participation of serine proteases in the establishment of the interaction between both organisms, which is the case of VviSBT4.19 X1. The gene expression of this subtilase increases up to 300-fold 6 hours after ‘Regent’ inoculation with P. viticola. Nowadays, no information is available about the participation of subtilases in grapevine response to E. necator and B. cinerea infection. In the present study, the gene expression profile of VviSBT4.19 X1 in the first hours of ‘Regent’ inoculation with E. necator and B. cinerea was analysed to understand its response towards different pathogenic agents.
葡萄藤(Vitis vinifera L.)是世界上栽培最多的果树之一,具有很高的经济价值。白粉病和灰霉菌病分别由赤霉和灰霉菌引起,属于最具破坏性的疾病,可通过在一个生长季节使用几种杀菌剂加以控制。一种更可持续、更环保的虫害防治方法与育种计划的发展有关,即将具有天然抗性的美洲和亚洲葡萄品种与易受真菌或卵菌病原体引起的疾病的葡萄品种杂交。因此,获得了既具有良好浆果品质又具有高度抗葡萄病原菌能力的葡萄新品种。其中一个例子是葡萄品种“丽晶”,它对E. necator和Plasmopara viticola具有很高的耐受性。为了确保育种计划中持久的抗性渗入,充分了解葡萄藤的防御机制至关重要。葡萄- p的研究进展。viticola的病理系统表明丝氨酸蛋白酶参与了两种生物之间相互作用的建立,VviSBT4.19 X1就是这种情况。这种枯草酶的基因表达在Regent接种葡萄假单胞菌6小时后增加了300倍。目前,尚无关于枯草酶参与葡萄对necator e和cinerea感染的反应的资料。本研究分析了VviSBT4.19 X1在“Regent”接种E. necator和B. cinerea后第1小时的基因表达谱,以了解其对不同病原体的反应。
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引用次数: 4
Volatile and sensory characterization of white wines from three minority Portuguese grapevine varieties 三种葡萄牙少数葡萄品种白葡萄酒的挥发性和感官特性
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203501049
S. Piras, J. Brazão, J. Ricardo-da-Silva, O. Anjos, I. Caldeira
This work focused on the characterization of the volatile compounds and sensory profile of white wines produced from three minority grapevine varieties of Portugal namely ‘Malvasia’ (Colares), ‘Verdelho’ and ‘Galego Dourado’. The characterization took place using sensory and gas chromatography analysis. Furthermore, the data obtained were analysed through the use of multivariate analysis, which made it possible to evaluate the similarities and dissimilarities between the varieties. The results obtained show a differentiation of the wines produced from each grapevine variety but above all a differentiation of the two vintages was verified. The results obtained, both from a sensory and a chemical point of view, show an interesting oenological potential of these varieties, but still require further studies, in order to evaluate the influence of climatic effects on the profile of volatile compounds and also on the sensory profile.
这项工作的重点是表征挥发性化合物和白葡萄酒的感官特征,这些白葡萄酒是由葡萄牙的三个少数葡萄品种,即“Malvasia”(Colares),“Verdelho”和“Galego Dourado”生产的。使用感官和气相色谱分析进行表征。此外,通过使用多变量分析对获得的数据进行分析,从而可以评估品种之间的异同。所获得的结果显示了不同葡萄品种生产的葡萄酒的差异,但最重要的是两个年份的差异得到了证实。从感官和化学的角度来看,所获得的结果显示了这些品种的有趣的酿酒潜力,但仍需要进一步的研究,以评估气候对挥发性化合物和感官特征的影响。
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引用次数: 6
An overview on botrytized wines 霉变葡萄酒概述
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203502076
Georgios Kallitsounakis, S. Catarino
Noble rot wine is a specific type of sweet wine that derives from the infection of grape berries by a fungus called Botrytis cinerea. These wines are produced in specific wine regions around the world, with Sauternes region of France and Tokay region of Hungary being the most famous ones. The purpose of the current article is to provide a systematic review on the different stages of botrytized wines production, including a detailed analysis of the technical aspects involved. Specifically, it describes the process and development of berry infection by B. cinerea, and special emphasis is given to the main stages and operations of winemaking, conservation, aging and stabilization. A complex combination of a number of parameters (e.g., very specific environmental conditions) explains the rarity of noble rot occurrence and highlights the uniqueness of botrytized wines.
贵族腐酒是一种特殊类型的甜葡萄酒,它是由葡萄浆果被一种叫做葡萄孢菌的真菌感染而产生的。这些葡萄酒产于世界各地的特定葡萄酒产区,其中法国的苏特恩地区和匈牙利的托凯地区是最著名的。本文的目的是提供一个系统的审查不同阶段的葡萄酒生产,包括技术方面的详细分析所涉及的。具体来说,它描述了葡萄球菌侵染浆果的过程和发展,并特别强调了酿酒、保存、陈酿和稳定的主要阶段和操作。许多参数(例如,非常特殊的环境条件)的复杂组合解释了贵族腐烂发生的罕见性,并突出了霉变葡萄酒的独特性。
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引用次数: 9
Spread parameters of the borerXylotrechus Arvicola(Olivier) (Coleoptera: Cerambycidae) in a ‘Tempranillo’ vineyard in La Rioja (Spain): A long-term study 在西班牙拉里奥哈(La Rioja)的“Tempranillo”葡萄园中,小蠹蛾(鞘翅目:天牛科)的传播参数:一项长期研究
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203502148
R. Ocete, I. Armendâriz, C. Ocete, L. Maistrello, J. M. Valle, Álvaro Rodríguez, L. Usategui
Xylotrechus arvicola(Olivier) (Coleoptera: Cerambycidae) is a polyphagous xylophagous beetle that is becoming a pest of increasing importance for vineyards in Spain, also because of the wood fungi developing in the galleries excavated by its larvae, which cause a progressive decline of the affected grapevines, until death. Between 1993 and 2015, a survey of the infestation caused byX. arvicolaand the symptoms caused by pathogenic wood fungi was performed in a ‘Tempranillo’ variety vineyard in La Rioja region (Spain). Maps showing the overtime spread of the borer and the diffusion of symptoms of grapevine decline andEutypadieback were obtained. Results indicated that the borer colonization began in the centre of the plot, followed by the first symptoms caused by the wood fungi a few years later. The statistical analysis showed that the evolution of infestation is characterized by a linear increase of new holes whereas the pattern of their allocation in the vines follows a bimodal distribution which, to some extent, can be simulated by a Poisson’s model. Based on these observations, a methodology to estimate the state of the infestation over time is proposed. The procedure - based on a linear regression of the average number of holes per vine over a set of years - can be applied in a relatively simple way and provides the probability for a grapevine to have a certain number of exit holes in a definite year with a mean error of around 5%.
Xylotrechus arvicola(Olivier)(鞘翅目:天牛科)是一种多食性食木甲虫,对西班牙葡萄园的危害越来越大,也是因为它的幼虫在挖掘的通道中生长的木真菌会导致受影响的葡萄藤逐渐衰退,直到死亡。1993年至2015年间,一项关于x。在西班牙拉里奥哈地区的一个“丹魄”品种葡萄园中进行了由致病性木真菌引起的症状试验。获得了显示蛀虫随时间传播的地图,以及葡萄藤衰退和枯死症状的扩散。结果表明,蛀虫的定植始于地块的中心,几年后才出现木真菌引起的初步症状。统计分析表明,侵染的演变特征是新孔的线性增加,而它们在葡萄藤上的分布模式遵循双峰分布,在一定程度上可以用泊松模型来模拟。根据这些观察结果,提出了一种估计虫害随时间变化的方法。该方法——基于一组年份内每棵葡萄树的平均孔数的线性回归——可以以一种相对简单的方式应用,并提供葡萄树在特定年份具有一定数量的出口孔的概率,平均误差约为5%。
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引用次数: 2
Human resource practices and performance in small Spanish wineries, and their evolution with age and size 西班牙小型酒庄的人力资源实践和绩效,以及它们随着年龄和规模的演变
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/ctv20203502107
J. Ferrer, Silvia Abella-Garcés, María Teresa Maza-Rubio
This research aims to cover the existing gap in knowledge regarding human resource management practices in winery businesses. Three of the most important practices in this field - recruitment and selection, training and development, and remuneration - and their relationship with performance in small family and non-family wineries as well as the differences in those businesses’ behaviours according to their age and size were analysed. The analysis was based on a 2016 database containing 339 Spanish wine sector SMEs, and a multivariate Bayesian regression methodology was applied. The results demonstrate a lower level of human resource management practices in small family businesses and a stronger relationship with performance than in non-family businesses. The results also show that human resource management varies according to the age and size of the company, indicating an inverted U-shaped relationship with size. On the one hand, these results highlight the importance of human resource practices in the environment of a small winery. These practices have not usually been considered as drivers of performance in small family firms. On the other hand, the results can be useful for the managers of such firms, both in the wine industry and in general, as they highlight the human resource practices that could improve the performance of those entities. The paper contributes to filling the existing gap in the literature related to small family businesses.
本研究的目的是覆盖现有的差距知识,有关人力资源管理实践在酒庄企业。本文分析了这一领域中最重要的三个实践——招聘和选择、培训和发展以及薪酬——以及它们与小型家族和非家族酒庄业绩的关系,以及这些企业根据其年龄和规模而产生的行为差异。该分析基于2016年包含339家西班牙葡萄酒行业中小企业的数据库,并采用多元贝叶斯回归方法。结果表明,与非家族企业相比,小型家族企业的人力资源管理实践水平较低,与绩效的关系更强。研究结果还表明,人力资源管理随公司年龄和规模的不同而不同,与规模呈倒u型关系。一方面,这些结果突出了人力资源实践在小酒厂环境中的重要性。这些做法通常不被认为是小型家族企业业绩的驱动因素。另一方面,研究结果对葡萄酒行业和一般企业的管理人员都是有用的,因为它们突出了可以提高这些实体绩效的人力资源实践。本文的研究有助于填补目前有关小型家族企业的文献空白。
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引用次数: 10
Agronomic performance of ‘Sauvignon Blanc’ variety trained in Y-trellis and vertical shoot position trellis in a high-altitude region of southern Brazil 在巴西南部高海拔地区,“长相思”品种在y型棚架和垂直茎位棚架上的农艺表现
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203502167
José Luiz Marcon, D. A. Wurz, A. Brighenti, Ricardo Allebrandt, B. Bem, L. Rufato
The objective of the present work was to compare the effect of the training systems Y-trellis (Y) and Vertical Shoot Position trellis (VSP) on the production efficiency and chemical characteristics of grapes and wine from ‘Sauvignon Blanc’ cultivated in high altitude regions of Santa Catarina State, Brazil. The study was carried out in 2013, 2014 and 2015 harvests in a commercial vineyard located in the municipality of São Joaquim - SC (28º 17 ’38 ”S and 49º 55’54” O, altitude 1350 m). The obtained data were subjected to analysis of variance (ANOVA) with F Test (p ≤ 0.05). The relationship between the vegetative growth and the yield was influenced by the training system. For the ‘Sauvignon Blanc’ cultivar, the yield was 93% higher in the Y-trellis training system, resulting in better vegetative-productive balance. The technological and phenolic maturation of the grapes were similar in both training systems. The results of this study show that the Y-trellis training system is an alternative to use in Santa Catarina altitude regions because it provides an increased productivity on the ‘Sauvignon Blanc’ variety without compromising the composition of grapes, when comparing to the VSP training system.
本研究的目的是比较Y型棚架(Y)和垂直梢位棚架(VSP)训练系统对巴西圣卡塔琳娜州高海拔地区种植的“长相思”葡萄和葡萄酒的生产效率和化学特性的影响。研究于2013年、2014年和2015年在位于若阿金- SC市(28º17′38”S和49º55′54”O,海拔1350 m)的一个商业葡萄园进行,所得数据采用F检验进行方差分析(ANOVA) (p≤0.05)。营养生长与产量的关系受栽培制度的影响。对于“长相思”品种,在y型棚架训练系统中,产量提高了93%,从而实现了更好的营养-生产平衡。在两种训练系统中,葡萄的工艺和酚成熟是相似的。这项研究的结果表明,y -格架训练系统是在圣卡塔琳娜高原地区使用的一种替代方案,因为与VSP训练系统相比,它在不影响葡萄成分的情况下提高了“长相思”品种的生产力。
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引用次数: 3
Effects of winemaking on ‘Marselan’ red wines: volatile compounds and sensory aspects 酿酒对马塞兰红葡萄酒的影响:挥发性化合物和感官方面
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203502063
Marianna Pozzatti, C. Guerra, Guilherme Martins, Ingrid D Dos Santos, R. Wagner, M. Ferrão, V. Manfroi
Winemaking processes and volatile compounds perform an important role in contributing to the quality of wines. ‘Marselan’ is a red grape variety grown in several countries such as France, Brazil and China. This variety has gained international interest in recent years. The volatile profile of Brazilian ‘Marselan’ red wines during various winemaking processes was studied. Four processes were applied: thermovinification, integrale vinification, classic winemaking and classic winemaking pretreated with ultrasound. The wines were characterized with conventional physicochemical analyses, volatile composition and sensory analysis. Sixty-three volatile compounds were identified and semi-quantified. The wines presented different sensory characteristics. The thermovinification and classic winemaking with ultrasound application methods showed a large presence of esters contributing to a fruity and overmaturation aroma in the volatile profile and sensory analysis. An increased presence of vegetal and sulfurous aromas was observed in the wines from the classic winemaking and integrale vinification processes. This effect was most likely attributed to the lower influence of esters which generated a greater perception of other compounds and conferred these aromas. The differences can be directly linked to the various extraction rates in the vinification processes, which result in different interactions between the compounds.
酿酒过程和挥发性化合物对葡萄酒的品质起着重要的作用。“玛瑟兰”是一种红葡萄品种,在法国、巴西和中国等几个国家都有种植。近年来,这种品种引起了国际上的兴趣。研究了巴西“马塞兰”红葡萄酒在不同酿酒过程中的挥发性特征。采用四种工艺:热发酵、整体发酵、经典酿酒和超声预处理经典酿酒。采用常规理化分析、挥发性成分分析和感官分析对葡萄酒进行了表征。鉴定并半定量了63种挥发性化合物。葡萄酒呈现出不同的感官特征。热发酵和经典的超声波酿酒方法表明,在挥发性特征和感官分析中,大量酯类物质的存在导致了果香和过熟香气。在经典的葡萄酒酿造和完整的酿造过程中,植物和硫磺的香气增加了。这种效果很可能是由于酯类的影响较低,酯类对其他化合物产生了更大的感知,并赋予了这些香气。这些差异可以直接与酿造过程中不同的提取率有关,这导致化合物之间的相互作用不同。
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引用次数: 8
Evaluation of rootstocks for the ‘Verdejo Negro’ cultivar ' Verdejo Negro '品种砧木的评价
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-01-01 DOI: 10.1051/ctv/20203502120
M. D. Loureiro, P. Moreno-Sanz, B. Suárez
Grapevine (Vitis vinifera L.) cultivation in Asturias (Northwestern Spain) was on the brink of extinction in the last century. Nevertheless, the present century is witnessing its rebound as a consequence of the recognition of the Cangas Wine Designation of Origin. The long period of abandonment has resulted in a lack of information about the most suitable rootstocks for the attainment of optimal quality. In this study, the agronomical and oenological parameters of the ‘Verdejo Negro’ cultivar grafted on three rootstocks (‘196-17 C’, ‘101-14 MG’ and ‘3309 C’) were studied. Three repetitions of ten vines for each rootstock were disposed in a completely randomized design. Agronomical (phenology, yield, pruning weight and Ravaz index) and enological (berry, must and wine composition) parameters were measured, and the elaborated wines were sensorially evaluated. Concerning phenology, the ‘101-14 MG’ rootstock advanced the veraison in the last two years of the study. The year significantly influenced many of the agronomical and enological parameters, whereas the rootstock only affected the Weaver index, which was higher for the ‘101-14 MG’ rootstock, indicating a more advanced maturation with this rootstock, and at the wine volatile acidity, higher for ‘3309 C’ wines. When the wines of ‘Verdejo Negro’ grafted on the three studied rootstocks were sensorially evaluated, few differences were found. Wines from ‘101-14 MG’ scored slightly better for nose parameters, although the overall judgment was very similar for all the wines. Considering the small effect of the rootstocks on the wines of ‘Verdejo Negro’, there is no clear recommendation at present. But it is important to take into account the effects of climate change, since the advance of maturation induced by the ‘101-14 MG’ rootstock may hinder the production of balanced wines of the ‘Verdejo Negro’ cultivar in the future. The predicted earlier ripening may lead to grapes with excessive sugar level, low acidity and a decoupling of phenolic and technological maturity.
葡萄藤(Vitis vinifera L.)种植在阿斯图里亚斯(西班牙西北部)在上个世纪濒临灭绝。尽管如此,本世纪见证了它的反弹,因为承认了坎加斯葡萄酒的原产地名称。长时间的废弃导致缺乏关于获得最佳质量的最合适砧木的信息。本研究以‘Verdejo Negro’为材料,研究了嫁接在‘196 ~ 17c’、‘101 ~ 14mg’和‘3309c’3根砧木上的‘Verdejo Negro’品种的农艺和酿酒参数。采用完全随机设计,每根砧木重复3次,每根10根。测量了农艺参数(物候、产量、修剪重量和拉瓦兹指数)和酿酒参数(浆果、葡萄和葡萄酒成分),并对酿造的葡萄酒进行了感官评价。在物候学方面,‘101-14 MG’砧木在过去两年的研究中取得了进步。年份显著影响了许多农艺和酿酒参数,而砧木只影响韦弗指数,‘101-14 MG’砧木的韦弗指数更高,表明该砧木成熟得更早,而在葡萄酒挥发性酸度方面,‘3309 C’葡萄酒的韦弗指数更高。当“Verdejo Negro”嫁接到三种砧木上的葡萄酒进行感官评估时,几乎没有发现差异。虽然所有葡萄酒的总体评价都非常相似,但“101- 14mg”级别的葡萄酒在嗅觉参数上得分略高。考虑到根茎对“Verdejo Negro”葡萄酒的影响很小,目前还没有明确的推荐。但考虑到气候变化的影响也很重要,因为101- 14mg砧木导致的成熟期提前可能会阻碍“Verdejo Negro”品种未来平衡葡萄酒的生产。预测的早熟可能导致葡萄含糖量过高,酸度低,酚类物质与工艺成熟度脱钩。
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Ciencia E Tecnica Vitivinicola
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