Pub Date : 2022-03-25DOI: 10.1007/s12042-022-09313-x
A. L. G. L. Peres, R. Coletta, J. S. Soares, M. Menossi
{"title":"Gene Silencing Using Artificial miRNA in Sugarcane","authors":"A. L. G. L. Peres, R. Coletta, J. S. Soares, M. Menossi","doi":"10.1007/s12042-022-09313-x","DOIUrl":"https://doi.org/10.1007/s12042-022-09313-x","url":null,"abstract":"","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"15 1","pages":"111 - 120"},"PeriodicalIF":2.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44407022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-22DOI: 10.1007/s12042-022-09310-0
P. Adu-Gyamfi, A. Akpertey, M. Barnor, A. Dadzie, S. Osei-Akoto, F. Padi
{"title":"Genotype × Environment Interaction Effects on Cashew (Anacardium occidentale L.) Flower Sex Type Expression","authors":"P. Adu-Gyamfi, A. Akpertey, M. Barnor, A. Dadzie, S. Osei-Akoto, F. Padi","doi":"10.1007/s12042-022-09310-0","DOIUrl":"https://doi.org/10.1007/s12042-022-09310-0","url":null,"abstract":"","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"15 1","pages":"157 - 170"},"PeriodicalIF":2.0,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44207063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-26DOI: 10.1007/s12042-021-09308-0
Chunni Liu, Yang Huang, Yu Liang
Camellia limonia is a kind of rare plant with high economic and medicinal value. It’s a plant species growing in calcareous soil of limestone, which means they can grow well in high calcium environment. Compared with C. limonia, Camellia nitidissima is a plant growing in acidic soil, which grows slowly or even dies in high calcium environment. However, there are few studies on C. limonia in karst soil environment and its adaptation mechanism is no clear. In this study, we found that under high calcium treatment, the chlorophyll content and leaf areas of C. limonia increased, while those of C. nitidissima decreased. The photosynthetic efficiency of C. limonia was more stable and higher than C. nitidissima. Compared with C. nitidissima, the conductance was larger and the degree of leaf shrinkage was smaller in C. limonia. The metabolomics analysis of both leaves showed that the kaempferol-3-o-rutinoside, tyrosol, 6-o-methyldeacetylisoipecoside and (r)-mandelic acid are the main differently metabolic compounds. The results of metagenomics in karst soil showed that the secretion (propylene oxide) of Acaryochloris marina in rhizosphere is closely related to the adaptation of C. nitidissima in high-calcium regions. Through metabolomics and metageomics integrative analysis, flavone and flavonol biosynthesis were suggested to be the main regulation pathway, which were regulated by metabolites apigenin, kaempferol, astragalin, isoquercitrin and genes TT7, UGT78D1, UGT78D2. This metabolic pathway involved the synthesis of flavonoids. Flavonoids have the characteristics of drought resistance and salt resistance, which play an important role in the adaptation of C. limonia in karst high calcium environment. This omics study identified key regulation metabolites and genes for Camellia and provided important basis for the adaptive mechanism of plants to adapt to the high-calcium environment and the protection of Camellia species.
{"title":"Adaptive Mechanism Exploration of Camellia limonia in Karst High Calcium Environment by Integrative Analysis of Metabolomics and Metagenomics","authors":"Chunni Liu, Yang Huang, Yu Liang","doi":"10.1007/s12042-021-09308-0","DOIUrl":"https://doi.org/10.1007/s12042-021-09308-0","url":null,"abstract":"<p><i>Camellia limonia</i> is a kind of rare plant with high economic and medicinal value. It’s a plant species growing in calcareous soil of limestone, which means they can grow well in high calcium environment. Compared with <i>C. limonia</i>, <i>Camellia nitidissima</i> is a plant growing in acidic soil, which grows slowly or even dies in high calcium environment. However, there are few studies on <i>C. limonia</i> in karst soil environment and its adaptation mechanism is no clear. In this study, we found that under high calcium treatment, the chlorophyll content and leaf areas of <i>C. limonia</i> increased, while those of <i>C. nitidissima</i> decreased. The photosynthetic efficiency of <i>C. limonia</i> was more stable and higher than <i>C. nitidissima</i>. Compared with <i>C. nitidissima</i>, the conductance was larger and the degree of leaf shrinkage was smaller in <i>C. limonia</i>. The metabolomics analysis of both leaves showed that the kaempferol-3-o-rutinoside, tyrosol, 6-o-methyldeacetylisoipecoside and (r)-mandelic acid are the main differently metabolic compounds. The results of metagenomics in karst soil showed that the secretion (propylene oxide) of <i>Acaryochloris marina</i> in rhizosphere is closely related to the adaptation of <i>C. nitidissima</i> in high-calcium regions. Through metabolomics and metageomics integrative analysis, flavone and flavonol biosynthesis were suggested to be the main regulation pathway, which were regulated by metabolites apigenin, kaempferol, astragalin, isoquercitrin and genes <i>TT7</i>, <i>UGT78D1</i>, <i>UGT78D2</i>. This metabolic pathway involved the synthesis of flavonoids. Flavonoids have the characteristics of drought resistance and salt resistance, which play an important role in the adaptation of <i>C. limonia</i> in karst high calcium environment. This omics study identified key regulation metabolites and genes for <i>Camellia</i> and provided important basis for the adaptive mechanism of plants to adapt to the high-calcium environment and the protection of <i>Camellia</i> species.</p>","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"357 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138508400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-22DOI: 10.21203/rs.3.rs-995211/v1
Gouri Priya Ranjith, Jisha Satheesan, K. Sabu
Centella asiatica is a widely spread herb mostly found in the tropics having extensive medicinal values. Here, we report for the first time, transcriptome-wide characterization of miRNA profile from the leaves of C. asiatica using high-throughput Illumina sequencing. We identified 227 conserved and 109 putative novel miRNAs. Computational screening revealed potential mRNA targets for both the conserved and novel miRNAs encoding diverse transcription factors and enzymes involved in plant development, disease resistance, metabolic and signaling pathways. Gene ontology annotation and KEGG analysis revealed the miRNA targets to be involved in a wide range of metabolomic and regulatory pathways. The differential expression of the miRNA encoding genes in diverse tissues was determined by real-time PCR analysis. We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. asiatica compared to its control. Furthermore, RLM-RACE experiments mapped miRNA-mediated cleavage at two of the mRNA targets. The present study represents the large-scale identification of microRNAs from C. asiatica and contributes to the base for the up-coming studies on miRNA-mediated gene regulation of plant secondary metabolite pathways in particular.
{"title":"Unraveling MicroRNA Mediated Gene Regulation in Centella Asiatica (L.) Urb. by High-Throughput Sequencing based Small-RNA Profiling.","authors":"Gouri Priya Ranjith, Jisha Satheesan, K. Sabu","doi":"10.21203/rs.3.rs-995211/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-995211/v1","url":null,"abstract":"\u0000 Centella asiatica is a widely spread herb mostly found in the tropics having extensive medicinal values. Here, we report for the first time, transcriptome-wide characterization of miRNA profile from the leaves of C. asiatica using high-throughput Illumina sequencing. We identified 227 conserved and 109 putative novel miRNAs. Computational screening revealed potential mRNA targets for both the conserved and novel miRNAs encoding diverse transcription factors and enzymes involved in plant development, disease resistance, metabolic and signaling pathways. Gene ontology annotation and KEGG analysis revealed the miRNA targets to be involved in a wide range of metabolomic and regulatory pathways. The differential expression of the miRNA encoding genes in diverse tissues was determined by real-time PCR analysis. We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. asiatica compared to its control. Furthermore, RLM-RACE experiments mapped miRNA-mediated cleavage at two of the mRNA targets. The present study represents the large-scale identification of microRNAs from C. asiatica and contributes to the base for the up-coming studies on miRNA-mediated gene regulation of plant secondary metabolite pathways in particular.","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"1 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43384686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.1007/s12042-021-09303-5
F. Garcia, Marcelo Rodrigues, J. Pennacchi, Ane Marcela das Chagas Mendonça, Daynara Aparecida Rodrigues Gonçalves, Nayara Cristina de Melo, J. P. R. A. D. Barbosa
{"title":"Sugarcane Resilience to Recurrent Water Deficit is Dependent on the Systemic Acclimation of Leaf Physiological Traits","authors":"F. Garcia, Marcelo Rodrigues, J. Pennacchi, Ane Marcela das Chagas Mendonça, Daynara Aparecida Rodrigues Gonçalves, Nayara Cristina de Melo, J. P. R. A. D. Barbosa","doi":"10.1007/s12042-021-09303-5","DOIUrl":"https://doi.org/10.1007/s12042-021-09303-5","url":null,"abstract":"","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"14 1","pages":"408 - 418"},"PeriodicalIF":2.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48823161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-23DOI: 10.1007/s12042-021-09300-8
Lijuan Yang, Shifang Li, Zimeng Zhang, Meiguang Lu
{"title":"Genome-Wide Identification of MicroRNAs that are Responsive to Virus/Viroid Infection in Nectarine Trees Through High-Throughput Sequencing","authors":"Lijuan Yang, Shifang Li, Zimeng Zhang, Meiguang Lu","doi":"10.1007/s12042-021-09300-8","DOIUrl":"https://doi.org/10.1007/s12042-021-09300-8","url":null,"abstract":"","PeriodicalId":54356,"journal":{"name":"Tropical Plant Biology","volume":"15 1","pages":"78 - 92"},"PeriodicalIF":2.0,"publicationDate":"2021-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48790802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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