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Determination of conifer age biomarker DAL1 interactome using Y2H-seq. 利用 Y2H-seq 确定针叶树年龄生物标志物 DAL1 的交互组。

IF 4.3 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2021-07-08 eCollection Date: 2021-01-01 DOI: 10.48130/FR-2021-0012

Xi Chen, Qianya Zhu, Yumeng Nie, Fangxu Han, Yue Li, Harry X Wu, Shihui Niu

Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.

年龄是一种复杂的生理信号，它确保了生物体不同发育阶段的顺序。裸子植物和被子植物对衰老途径的调控似乎有所不同。我们之前发现 DAL1 是一种保守的针叶树年龄生物标志物，它在松树从无性生殖阶段向生殖生活史阶段过渡的过程中起着至关重要的作用。因此，阐明与 DAL1 相关的特定相互作用事件是了解年龄如何驱动针叶树发育的关键。通过大规模的酵母双杂交（Y2H）分析和下一代高通量测序（Y2H-seq），我们在松树中鉴定出了135个与PtDAL1相互作用的蛋白质。我们的研究发现，PtDAL1 相互作用蛋白显示出与老化相关的模块，其复杂的相互作用网络由转录因子（TF）、转录调节因子（TR）和激酶组成。这些互作蛋白的产生是对各种植物激素和环境信号的响应，很可能参与了木质部的形成、针叶的发育、卵磷脂萜类化合物的生物合成和生殖发育。在这项研究中，我们提出了一种针叶树老化途径的新型调控模式，即 PtDAL1 协调不同的环境刺激，并与相应的蛋白质相互作用，以调控适当的发育。

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引用次数: 0

The role of screening spinal MRI in children with solitary posterior fossa low-grade glial tumors. 脊柱磁共振成像筛查在单发后窝低级别胶质瘤患儿中的作用。

IF 2.1 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2019-11-15 Print Date: 2020-02-01 DOI: 10.3171/2019.9.PEDS19358

Jonathan Roth, Neal Fischer, David D Limbrick, Travis CreveCoeur, Liat Ben-Sira, Shlomi Constantini

Objective: Solitary posterior fossa low-grade glial tumors (SPFLGT) in children are rarely associated with leptomeningeal dissemination (LMD). To date, there are no clear guidelines regarding the role of screening and surveillance spinal MRI (sMRI) in children with SPFLGT, at diagnosis or during follow-up periods. The current study reviews a cohort of children with SPFLGT, focusing on sMRI findings.

Methods: In this binational retrospective study, the authors analyzed 229 patients with SPFLGT treated and followed over 13 years. One hundred twelve children had at least 1 total sMRI screening or surveillance examination. One hundred seventeen had no sMRI, but did not present with clinical spinal signs or symptoms. Collected data included demographics, disease characteristics, radiology, pathology, and clinical follow-up data.

Results: For the 112 children with at least 1 sMRI, the mean duration from diagnosis to first sMRI was 11.73 ± 28.66 months (range 0-165 months). All sMRI scans were conducted as screening examinations, with no spinal-related symptoms. One patient was found to have a sacral intradural lesion concurrent to the brain tumor diagnosis. Over the course of 180 radiological and 533 clinical follow-up years for the 112 patients with sMRI, and 582 clinical follow-up years for the 117 patients with no sMRI, there were no additional cases with spinal tumor spread.

Conclusions: The yield of screening sMRI in the absence of cranial metastasis, or spinal symptoms, is extremely low. Because preoperative sMRI is recommended for medulloblastomas and ependymomas, it may be logical to acquire. During the follow-up period the authors recommend limiting sMRI in patients without symptoms suggesting a spinal lesion, in patients without known cranial metastases, or recurrence or residual SPFLGT.

目的：儿童孤立性后窝低级别胶质瘤（SPFLGT）很少伴有脑膜外播散（LMD）。迄今为止，对于 SPFLGT 患儿在诊断或随访期间进行脊柱磁共振成像（sMRI）筛查和监测的作用还没有明确的指导方针。本研究回顾了一组 SPFLGT 患儿的情况，重点关注 sMRI 的发现：在这项两国间的回顾性研究中，作者分析了 229 名接受治疗并随访 13 年的 SPFLGT 患者。112 名儿童至少接受了一次全面的 sMRI 筛查或监测检查。177名儿童没有接受过sMRI检查，但没有出现临床脊柱体征或症状。收集的数据包括人口统计学、疾病特征、放射学、病理学和临床随访数据：在至少进行过一次sMRI检查的112名儿童中，从确诊到首次进行sMRI检查的平均时间为（11.73 ± 28.66）个月（0-165个月）。所有sMRI扫描均为筛查性检查，无脊柱相关症状。其中一名患者在确诊脑瘤的同时发现骶骨硬膜内病变。在对112名接受sMRI检查的患者进行的180年放射学随访和533年临床随访，以及对117名未接受sMRI检查的患者进行的582年临床随访中，均未再发现脊柱肿瘤扩散的病例：结论：在没有颅骨转移或脊柱症状的情况下，sMRI 的筛查率极低。由于建议对髓母细胞瘤和上胚叶瘤进行术前sMRI检查，因此进行术前sMRI检查可能是合乎逻辑的。在随访期间，作者建议在没有症状提示脊柱病变的患者、没有已知颅骨转移、复发或残留 SPFLGT 的患者中限制进行 sMRI 检查。

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引用次数: 0

The non-native vascular flora of Campania (southern Italy) 坎帕尼亚(意大利南部)的外来维管植物群

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2018-07-04 DOI: 10.1080/11263504.2017.1338626

E. Del Guacchio, V. La Valva

Abstract We discuss several aspects of the alien flora of Campania (southern Italy), on the basis of an updated and annotated checklist. The non-native flora includes at present 346 taxa in 231 genera, representing 82 families. However, other 55 taxa were not found later than 1950. Trends in alien plants invasion throughout the investigated area are discussed. Results are therefore compared with similar data from other Italian regions and other European countries. In the Appendix, taxa are listed with essential synonymy, local and general distribution, status, biological form, habitat, diffusion mechanisms, potential threats, literature data, voucher specimens, and miscellaneous ethnobotanical, chronological and taxonomical notes. In addition, 49 escaped taxa are known only for Botanical and private gardens, while 66 taxa are regarded as doubtful aliens. By including also these two categories, the non-native flora of Campania reaches 516 taxa. In addition, numerous doubtful and erroneous indications were discussed. Finally, a reference list for the exotic regional flora from 1500 C.E. to present is proposed.

摘要本文根据一份更新和注释的清单，讨论了坎帕尼亚(意大利南部)外来植物区系的几个方面。外来植物区系包括82科231属346个分类群。另外55个分类群在1950年以后才被发现。讨论了调查区外来植物的入侵趋势。因此，将结果与意大利其他地区和其他欧洲国家的类似数据进行比较。在附录中，列出了分类群的基本同义词、局部和总体分布、现状、生物形态、栖息地、扩散机制、潜在威胁、文献资料、凭证标本以及杂项民族植物学、时间和分类注释。此外，49个逃逸类群仅在植物园和私人花园中已知，66个类群被认为是可疑的外来物种。再加上这两个分类，坎帕尼亚的外来植物群达到516个分类群。此外，还讨论了许多可疑和错误的迹象。最后，提出了1500年至今地区外来植物区系的参考目录。

引用次数: 6

Phytochemical studies, antioxidant activity and total phenolic content of Ferula communis L. organ extracts 阿魏器官提取物的植物化学研究、抗氧化活性及总酚含量

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2018-05-02 DOI: 10.1080/11263504.2018.14611696

Fatma Zohra Rahali, Sarra Kefi, I. B. Rebey, Jessica Tabart, C. Kevers, T. Franck, A. Mouithys-Mickalad, I. H. Sellami

引用次数: 0

Sulla respirazione dell'apparato radicale delle piante coltivate 关于根系植物的呼吸

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2009-09-14 DOI: 10.1080/11263504109440426

Francesco Crescini

Riassunto A conferma di quanto s'era supposto, la respirazione profonda e apparsa processo fisiologico assai comune. Infatti, essa e stata accerta'a — spesso anche in fasi e periodi diversi del ciclo vitale della stessa pianta — in tutte le specie saggiate (circa 40 riferibili a 16 famiglie). Nelle piante legnose, il processo respiratorio profondo e risultato della stessa natura che nelle specie erbacee. Il processo respiratorio profondo e il processo respiratorio superficiale ipogco acquistano valori e significati diversi sia dal lato fisiologico che agronomico secondo la forma coltivata (specie, individuo), le condizioni termiche e la natura del mezzo asfittico del suolo. Per tanto, vuoi nelle piante erbacee che legnose coltivate, e dato osservare una grande variabilita d'adattamento i cui estremi incidono sulle specie che normalmente vivono coll'apparato radicale in terreno idropico cioe in mezzo asfittico costituito da eccesso d'acqua immota e, per contro, sulle specie sensibili — fino a un certo punt...

深度呼吸似乎是一个非常常见的生理过程。事实上，它在所有被测试的物种中都被发现——通常是在同一株植物生命周期的不同阶段和时期——大约有40种(涉及16个科)。在木本植物中，深呼吸过程与草本植物具有相同的性质。根据作物(物种、个体)、热条件和土壤酸性介质的性质，深呼吸和浅呼吸过程在生理和农业两方面都有不同的价值和意义。因此,无论是在生长的木质草本植物,因为看到一种非常极端的适应variabilita影响通常生活彻底coll’apparato idropico地形中即在半消耗性疾病是由过量水immota和敏感,另一方面,物种—在某种程度平底...

引用次数: 0

Località Nuove di Piante Italiane 意大利植物的新地点

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2009-09-14 DOI: 10.1080/11263503309437907

A. Fiori

引用次数: 4

Sezione Veneta (Padova) 威尼斯节(帕多瓦)

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2009-09-14 DOI: 10.1080/11263504309439860

G. G. I. Presidente

引用次数: 0

Structural and Functional Analysis of the pgip Promoter pgip启动子的结构与功能分析

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2009-09-14 DOI: 10.1080/11263509509440956

A. Devoto, F. Leckie, R. Masiello, F. Cervone, G. Lorenzo

引用次数: 0

Sezione Piemontese (Torino) 皮埃蒙特地区(都灵)

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2009-09-14 DOI: 10.1080/11263504109439801

A. C. I. Segretario, F. V. I. Presidente

引用次数: 0

Sulle Cause Determinanti La Formazione Di Tumori in Ibridi Di Nicotiana 关于烟草杂交肿瘤形成的决定性原因

IF 2 4区生物学 Q3 PLANT SCIENCES

Plant Biosystems

Pub Date : 2009-09-14 DOI: 10.1080/11263506309430205

Claudio P. Longo

Abstract Cause of tumor formation in NICOTIANA hybrids. Further serological studies. — Additional researches have been carried out about genetical tumors in genus Nicotiana. By means of serological procedure the affinity among pairs of species that give rise to tumorous and non-tumorous hybrids has been tested, considering also their phyletic interrelation. Using ion exchange chromatography a qualitative analysis of the total proteins of tumorous and non-tumorous tissue of hybrids and of normal leaves taken from hybrids and their parental species was carried out. Serological analysis of affinity between the species tested gave following results: 1) higher serological affinity was found between species belonging to the same subgenus than between species belonging to different subgenera. 2) serological affinity between pairs of species giving rise to tumorous hybrids is higher than the affinity observed between other pairs of species that give rise to normal hybrids in spite of the same sistematic position....

烟草杂交种肿瘤形成的原因。进一步的血清学研究。-对烟草属基因肿瘤进行了进一步研究。通过血清学方法，对产生肿瘤和非肿瘤杂交的物种对之间的亲和力进行了测试，同时考虑到它们的种系相互关系。采用离子交换色谱法对杂交植物的肿瘤组织和非肿瘤组织以及杂交植物及其亲本正常叶片的总蛋白进行了定性分析。血清学分析结果表明:1)同一亚属种间血清学亲和力高于不同亚属种间血清学亲和力。2)产生肿瘤杂交种的物种对之间的血清学亲和力高于其他产生正常杂交种的物种对之间的亲和力，尽管系统位置相同....

引用次数: 0

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