Pub Date : 2023-09-30DOI: 10.1080/11263504.2023.2257720
Ammar B. Altemimi, Muthanna J. Mohammed, Zina T. Alkanan, Shaymaa H. Abdulrahman, Shatha A. Allaitha, Tarek Gamal Abedelmaksoud, Qausar Hamed ALKaisy, Mazin A. A. Najm, Francesco Cacciola, P. Vasantha Srinivasan
AbstractIn this study, the bioactive components of Citrullus colocynthis extracts were analyzed in view of their potential use as antioxidant/antibacterial agent. The bioactive compounds were extracted in different organic solvents (hexane, ethanol and ethyl acetate) and analyzed by column chromatography, thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectrophotometry (GC-MS). Three distinct fractions were collected and subsequently examined, one from ethyl acetate extraction (I) and two from ethanolic extraction (II and III). p-Coumaric acid was the major phenolic acid from the ethyl acetate extracts (0.11 ± 0.023 mg/kg) while resorcinol was the major phenolic compound from the ethanol extracts (25.00 ± 3.21 mg/kg). Twenty-four bioactive compounds were identified by GC-MS analysis where 1-docosanol pentafluoropropionate was the major bioactive compound in ethanol extracts. Among the ethanol extracts, the highest antioxidant activity was found in fraction III. The highest anti-bacterial activity values, measured in terms of the size of the inhibition zone (mm) against Bacillus cereus and Staphylococcus aureus, i.e. 24 ± 1.62 and 22 ± 2.19 mm, respectively, were attained at 24 µg/mL for fractions III and I. From the results achieved in this work, ethanol extracts of C. colocynthis aerial parts could be considered promising as antioxidant and anti-infectious agents.Keywords: Citrullus colocynthis aerial partsbioactive compoundshPLCGC-msantioxidantantibacterial Authors’ contributionAmmar B. Altemimi: Conceptualization, Writing—Original draft, Project administration. Muthanna J. Mohammed: Investigation. Zina T. Alkanan: Investigation. Shaymaa H. Abdulrahman, Investigation. Shatha A. Allaitha: Investigation. Tarek Gamal Abedelmaksoud: Writing—Reviewing and editing, Supervision. Qausar Hamed ALKaisy: Investigation. Qausar Hamed ALKaisy: Investigation. Mazin A. A. Najm: Writing—Reviewing and editing. Francesco Cacciola: Conceptualization, Writing—Reviewing and editing, Supervision. P. Vasantha Srinivasan: Writing—Reviewing and editing, Supervision.Disclosure statementThe authors declare no conflict of interest.
摘要本研究分析了香瓜提取物的生物活性成分，探讨了其作为抗氧化/抗菌药物的潜力。在不同的有机溶剂(己烷、乙醇和乙酸乙酯)中提取生物活性化合物，并采用柱层析、薄层析、高效液相色谱和气相色谱-质谱联用(GC-MS)分析。分别从乙酸乙酯提取液(ⅰ)和乙醇提取液(ⅱ和ⅲ)中提取3个不同的组分，乙酸乙酯提取液中主要的酚类化合物为对香豆酸(0.11±0.023 mg/kg)，乙醇提取液中主要的酚类化合物为间苯二酚(25.00±3.21 mg/kg)。通过气相色谱-质谱分析鉴定出24种生物活性化合物，其中1-二十二醇五氟丙酸是乙醇提取物中的主要生物活性化合物。在乙醇提取物中，抗氧化活性最高的部位为ⅲ。对蜡样芽孢杆菌和金黄色葡萄球菌的抑菌区大小(mm)测定表明，在24µg/mL浓度下，三组分和一组分的抑菌区大小分别为24±1.62和22±2.19 mm，具有较高的抑菌活性。关键词:甜瓜;空气成分;生物活性化合物;Muthanna J. Mohammed:调查。吉娜:调查。Shaymaa H. Abdulrahman，调查。Shatha A. Allaitha:调查。Tarek Gamal Abedelmaksoud:写作-审查和编辑，监督。Qausar Hamed ALKaisy:调查。Qausar Hamed ALKaisy:调查。Mazin A. A. Najm:写作-评论和编辑。Francesco Cacciola:概念化，写作审查和编辑，监督。P. Vasantha Srinivasan:写作-审查和编辑，监督。声明作者声明无利益冲突。
{"title":"Bioactive compounds of <i>Citrullus colocynthis</i> aerial parts: characterization and biological properties","authors":"Ammar B. Altemimi, Muthanna J. Mohammed, Zina T. Alkanan, Shaymaa H. Abdulrahman, Shatha A. Allaitha, Tarek Gamal Abedelmaksoud, Qausar Hamed ALKaisy, Mazin A. A. Najm, Francesco Cacciola, P. Vasantha Srinivasan","doi":"10.1080/11263504.2023.2257720","DOIUrl":"https://doi.org/10.1080/11263504.2023.2257720","url":null,"abstract":"AbstractIn this study, the bioactive components of Citrullus colocynthis extracts were analyzed in view of their potential use as antioxidant/antibacterial agent. The bioactive compounds were extracted in different organic solvents (hexane, ethanol and ethyl acetate) and analyzed by column chromatography, thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectrophotometry (GC-MS). Three distinct fractions were collected and subsequently examined, one from ethyl acetate extraction (I) and two from ethanolic extraction (II and III). p-Coumaric acid was the major phenolic acid from the ethyl acetate extracts (0.11 ± 0.023 mg/kg) while resorcinol was the major phenolic compound from the ethanol extracts (25.00 ± 3.21 mg/kg). Twenty-four bioactive compounds were identified by GC-MS analysis where 1-docosanol pentafluoropropionate was the major bioactive compound in ethanol extracts. Among the ethanol extracts, the highest antioxidant activity was found in fraction III. The highest anti-bacterial activity values, measured in terms of the size of the inhibition zone (mm) against Bacillus cereus and Staphylococcus aureus, i.e. 24 ± 1.62 and 22 ± 2.19 mm, respectively, were attained at 24 µg/mL for fractions III and I. From the results achieved in this work, ethanol extracts of C. colocynthis aerial parts could be considered promising as antioxidant and anti-infectious agents.Keywords: Citrullus colocynthis aerial partsbioactive compoundshPLCGC-msantioxidantantibacterial Authors’ contributionAmmar B. Altemimi: Conceptualization, Writing—Original draft, Project administration. Muthanna J. Mohammed: Investigation. Zina T. Alkanan: Investigation. Shaymaa H. Abdulrahman, Investigation. Shatha A. Allaitha: Investigation. Tarek Gamal Abedelmaksoud: Writing—Reviewing and editing, Supervision. Qausar Hamed ALKaisy: Investigation. Qausar Hamed ALKaisy: Investigation. Mazin A. A. Najm: Writing—Reviewing and editing. Francesco Cacciola: Conceptualization, Writing—Reviewing and editing, Supervision. P. Vasantha Srinivasan: Writing—Reviewing and editing, Supervision.Disclosure statementThe authors declare no conflict of interest.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136280081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-27DOI: 10.1080/11263504.2023.2258878
Thomas Kiebacher, Edwin Urmi
AbstractWe present an example of morphological convergence among two species of Scapania that were considered to be conspecific. We resurrect S. jensenii from synonymy with the morphologically and molecularly variable S. obcordata because our molecular data show that they are distantly related. Chloroplast trnL-trnF and nuclear ITS sequences resolved S. obcordata in sect. Curtae and S. jensenii in sect. Apiculatae sister to S. obscura and we detected morphological differences in secondary pigmentation and leaf anatomy. The similarity comprises the absence of a pronounced keel in vegetative leaves and a weakly defined stem cortex. These features are virtually unknown in other species of Scapania and probably result from convergence in adaptation to the same environmental conditions as both taxa inhabit alluvial plains characterised by regular disturbance by flooding and covering with sand and silt. While S. obcordata is widespread in the northern Holarctic and known from the Antarctic, S. jensenii is known from few localities in Greenland, mainland Norway, the Chukchi Peninsula and the Swiss Alps. Its rarity, the absence of female plants and the lack of genetic variability suggest that S. jensenii underwent a bottleneck event.Keywords: adaptive evolutionAlpsbryophytesdisturbanceScapaniaceaesex ratiotaxonomyDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.
{"title":"Intersectional polyphyly of the puzzling <i>Scapania obcordata</i> (Marchantiidae) suggests convergent evolution: Resurrection and European occurrence of <i>S. jensenii</i>","authors":"Thomas Kiebacher, Edwin Urmi","doi":"10.1080/11263504.2023.2258878","DOIUrl":"https://doi.org/10.1080/11263504.2023.2258878","url":null,"abstract":"AbstractWe present an example of morphological convergence among two species of Scapania that were considered to be conspecific. We resurrect S. jensenii from synonymy with the morphologically and molecularly variable S. obcordata because our molecular data show that they are distantly related. Chloroplast trnL-trnF and nuclear ITS sequences resolved S. obcordata in sect. Curtae and S. jensenii in sect. Apiculatae sister to S. obscura and we detected morphological differences in secondary pigmentation and leaf anatomy. The similarity comprises the absence of a pronounced keel in vegetative leaves and a weakly defined stem cortex. These features are virtually unknown in other species of Scapania and probably result from convergence in adaptation to the same environmental conditions as both taxa inhabit alluvial plains characterised by regular disturbance by flooding and covering with sand and silt. While S. obcordata is widespread in the northern Holarctic and known from the Antarctic, S. jensenii is known from few localities in Greenland, mainland Norway, the Chukchi Peninsula and the Swiss Alps. Its rarity, the absence of female plants and the lack of genetic variability suggest that S. jensenii underwent a bottleneck event.Keywords: adaptive evolutionAlpsbryophytesdisturbanceScapaniaceaesex ratiotaxonomyDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135536729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.1080/11263504.2023.2260820
Mahantesh Banni, M. Jayaraj
AbstractSida cordata is a perennial, spreading herb that is well-known for its ethnomedicinal uses in India, Pakistan, and other Asian countries. It is one of the most important plants in the Ayurveda and Siddha systems of medicine, and is known as Raajabalaa and Bhumibalaa in Sanskrit. The present study aims to develop a profile of bioactive compounds using GC-MS analysis and evaluate their anticancer and antioxidant potential. GC-MS analysis revealed the presence of 18 compounds in the petroleum ether extract, 14 compounds in the acetone extract, 12 compounds in the ethanol extract, and 6 compounds in the chloroform extract. Gamma-sitosterol is the most abundant compound found in the petroleum ether (18.48%), acetone (34.25%), and ethanol (25.17%) extracts. In the chloroform extract, 1,2-Benzenedicarboxylic acid is the most abundant compound, comprising 74.77% of the extract. The chloroform extract demonstrated significant in vitro cytotoxic activity against the HepG2 cell line, as determined by the MTT assay, with an IC50 value of 47.53 µg/mL. In vitro antioxidant potential was studied using DPPH and ABTS assays. However, the ethanol extract exhibited the highest scavenging activity, with IC50 values of 46.48 µg/mL and 94.17 µg/mL against their respective standards.Keywords: AntioxidantAyurvedaCytotoxicityEthnomedicineHepG2Sida cordataDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.
{"title":"Profile of bioactive compounds, <i>in vitro</i> anticancerous and antioxidant activity of stem extracts of <i>Sida cordata</i>","authors":"Mahantesh Banni, M. Jayaraj","doi":"10.1080/11263504.2023.2260820","DOIUrl":"https://doi.org/10.1080/11263504.2023.2260820","url":null,"abstract":"AbstractSida cordata is a perennial, spreading herb that is well-known for its ethnomedicinal uses in India, Pakistan, and other Asian countries. It is one of the most important plants in the Ayurveda and Siddha systems of medicine, and is known as Raajabalaa and Bhumibalaa in Sanskrit. The present study aims to develop a profile of bioactive compounds using GC-MS analysis and evaluate their anticancer and antioxidant potential. GC-MS analysis revealed the presence of 18 compounds in the petroleum ether extract, 14 compounds in the acetone extract, 12 compounds in the ethanol extract, and 6 compounds in the chloroform extract. Gamma-sitosterol is the most abundant compound found in the petroleum ether (18.48%), acetone (34.25%), and ethanol (25.17%) extracts. In the chloroform extract, 1,2-Benzenedicarboxylic acid is the most abundant compound, comprising 74.77% of the extract. The chloroform extract demonstrated significant in vitro cytotoxic activity against the HepG2 cell line, as determined by the MTT assay, with an IC50 value of 47.53 µg/mL. In vitro antioxidant potential was studied using DPPH and ABTS assays. However, the ethanol extract exhibited the highest scavenging activity, with IC50 values of 46.48 µg/mL and 94.17 µg/mL against their respective standards.Keywords: AntioxidantAyurvedaCytotoxicityEthnomedicineHepG2Sida cordataDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135059328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-15DOI: 10.1080/11263504.2023.2258882
Lu Gao, Charles Obinwanne Okoye, Yanfang Wu, Guizhu Wu, Jianxiong Jiang, Daolin Du, Yonglai Xue
ABSTRACTIonic liquids (ILs) are a promising alternative to the traditional organic solvents, but their toxic effects cause significant adverse environmental effects and their toxicity assessment have been suggested. The effect of the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]) on photosynthesis was studied in Arabidopsis thaliana. Apparent effects on chlorosis and inhibition of photosynthetic capacity were observed. As [Bmim][BF4] concentrations increased, a time-dose effect was also observed. The maximum quantum yield (Fv/Fm), and the actual quantum yield of photosystem II (ΦPSII), both decreased; at the same time, the expression of psbA, a key gene of the photosystem II electron transport, was reduced by [Bmim][BF4] treatment. The synthesis of the large and small subunits of Rubisco were not affected by treatment. On the other hand, Rubisco activase (RCA) was significantly suppressed at the transcriptional level. Our results suggest that photochemistry of PSII as well as Rubisco activation are involved in ILs resistance.Key words: Arabidopsis thaliana; [Bmim][BF4]Chlorophyll fluorescencegene transcript levelDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. Author contributionsLu Gao, Charles Obinwanne Okoye, and Guizhu Wu designed the study, performed the experiments, and wrote the article. Yanfang Wu and Jianxiong Jiang supervised the study. Daolin Du and Yonglai Xue conceived the study, reviewed and edited the manuscript. All authors read and approved the manuscript.AcknowledgmentsThis work was supported by the Key Research and Development Program of Zhenjiang City (SH20222011), and the Startup Support from Jiangsu University (13JDB017).
摘要离子液体是传统有机溶剂的一种很有前途的替代品，但其毒性对环境有显著的不良影响，其毒性评价已被提出。研究了离子液体1-丁基-3-甲基咪唑四氟硼酸盐([Bmim][BF4])对拟南芥光合作用的影响。对黄化和抑制光合能力有明显影响。随着[Bmim][BF4]浓度的增加，还观察到时间剂量效应。光系统II的最大量子产率(Fv/Fm)和实际量子产率(ΦPSII)均下降;同时，[Bmim][BF4]处理降低了光系统II电子传递的关键基因psbA的表达。Rubisco大小亚基的合成不受处理的影响。另一方面，Rubisco激活酶(RCA)在转录水平上被显著抑制。我们的研究结果表明PSII的光化学反应和Rubisco的活化参与了对il的抗性。关键词:拟南芥;[Bmim][BF4]叶绿素荧光基因转录水平免责声明作为对作者和研究人员的服务，我们提供此版本的已接受稿件(AM)。在最终出版版本记录(VoR)之前，将对该手稿进行编辑、排版和审查。在制作和印前，可能会发现可能影响内容的错误，所有适用于期刊的法律免责声明也与这些版本有关。lu Gao, Charles Obinwanne Okoye和Guizhu Wu设计了这项研究，进行了实验，并撰写了这篇文章。吴艳芳和姜建雄监督了这项研究。杜道林、薛永来负责研究构思、审稿和编辑工作。所有作者都阅读并认可了稿件。本研究得到镇江市重点研发计划(SH20222011)和江苏大学创业基金(13JDB017)的支持。
{"title":"Effect of 1-butyl-3-methylimidazolium tetrafluoroborate on photosynthesis in <i>Arabidopsis thaliana</i>","authors":"Lu Gao, Charles Obinwanne Okoye, Yanfang Wu, Guizhu Wu, Jianxiong Jiang, Daolin Du, Yonglai Xue","doi":"10.1080/11263504.2023.2258882","DOIUrl":"https://doi.org/10.1080/11263504.2023.2258882","url":null,"abstract":"ABSTRACTIonic liquids (ILs) are a promising alternative to the traditional organic solvents, but their toxic effects cause significant adverse environmental effects and their toxicity assessment have been suggested. The effect of the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]) on photosynthesis was studied in Arabidopsis thaliana. Apparent effects on chlorosis and inhibition of photosynthetic capacity were observed. As [Bmim][BF4] concentrations increased, a time-dose effect was also observed. The maximum quantum yield (Fv/Fm), and the actual quantum yield of photosystem II (ΦPSII), both decreased; at the same time, the expression of psbA, a key gene of the photosystem II electron transport, was reduced by [Bmim][BF4] treatment. The synthesis of the large and small subunits of Rubisco were not affected by treatment. On the other hand, Rubisco activase (RCA) was significantly suppressed at the transcriptional level. Our results suggest that photochemistry of PSII as well as Rubisco activation are involved in ILs resistance.Key words: Arabidopsis thaliana; [Bmim][BF4]Chlorophyll fluorescencegene transcript levelDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. Author contributionsLu Gao, Charles Obinwanne Okoye, and Guizhu Wu designed the study, performed the experiments, and wrote the article. Yanfang Wu and Jianxiong Jiang supervised the study. Daolin Du and Yonglai Xue conceived the study, reviewed and edited the manuscript. All authors read and approved the manuscript.AcknowledgmentsThis work was supported by the Key Research and Development Program of Zhenjiang City (SH20222011), and the Startup Support from Jiangsu University (13JDB017).","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135437177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-15DOI: 10.1080/11263504.2023.2258897
Lorenzo Pinzani, Gabriele Casazza, Gianni Bedini, Angelino Carta
AbstractKnowing the determinants of biodiversity is crucial to understanding the differentiation of living organisms. Several gene-flow limiting processes can drive divergence, including adaptive ecological differentiation or geographical isolation both constituting environmental-dependent reproductive barriers. Furthermore, the strength of these processes may be associated to the degree to which ecological niches are conserved and shared by phylogenetically related species. Here, we aim to disentangle these two alternatives reproductive barriers by evaluating the degree of ecological niche similarity among phylogenetically related species. We performed ecological niche modelling (ENM) on four species belonging to a recently diversified genus (Aquilegia, Ranunculaceae) occurring in montane environments of the Alpine and Apennine chains (southern Europe). Results show that niche differentiation among species is related to phylogenetic distinctiveness.Nevertheless, niche similarity tests indicated that the conditions selected by the species are more similar than expected from the null models, even though they are sparsely available in the respective ecological spaces, suggesting that the observed pattern may result from phylogenetic niche conservatism.These results, highlight that paleogeographic events in south-European mountains may have driven divergence by isolation in Aquilegia while partially retaining the possibly ancestral characters of the niches.Keywords: Aquilegiaecological niche modelsEuropean Alpine Systemniche conservatismniche overlapsimilarity testSpeciationDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.
{"title":"Geographical isolation as reproductive barrier in phylogenetically related <i>Aquilegia</i> species (Ranunculaceae)","authors":"Lorenzo Pinzani, Gabriele Casazza, Gianni Bedini, Angelino Carta","doi":"10.1080/11263504.2023.2258897","DOIUrl":"https://doi.org/10.1080/11263504.2023.2258897","url":null,"abstract":"AbstractKnowing the determinants of biodiversity is crucial to understanding the differentiation of living organisms. Several gene-flow limiting processes can drive divergence, including adaptive ecological differentiation or geographical isolation both constituting environmental-dependent reproductive barriers. Furthermore, the strength of these processes may be associated to the degree to which ecological niches are conserved and shared by phylogenetically related species. Here, we aim to disentangle these two alternatives reproductive barriers by evaluating the degree of ecological niche similarity among phylogenetically related species. We performed ecological niche modelling (ENM) on four species belonging to a recently diversified genus (Aquilegia, Ranunculaceae) occurring in montane environments of the Alpine and Apennine chains (southern Europe). Results show that niche differentiation among species is related to phylogenetic distinctiveness.Nevertheless, niche similarity tests indicated that the conditions selected by the species are more similar than expected from the null models, even though they are sparsely available in the respective ecological spaces, suggesting that the observed pattern may result from phylogenetic niche conservatism.These results, highlight that paleogeographic events in south-European mountains may have driven divergence by isolation in Aquilegia while partially retaining the possibly ancestral characters of the niches.Keywords: Aquilegiaecological niche modelsEuropean Alpine Systemniche conservatismniche overlapsimilarity testSpeciationDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135397643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-14DOI: 10.1080/11263504.2023.2259386
Hatice Emlik, Isik Didem Karagoz, Lawali Yabo Dambagi, Basak Simitcioglu, Ahmet Cakir
AbstractBreast cancer is one of the most common forms of cancer in women, therefore it has become necessary to find more effective and less toxic drugs in order to minimize the disadvantages of existing chemotherapeutic agents. The potential anticancer properties of masticadienonic acid, tirucallol and pistachionic acid, which are secondary metabolites isolated from Pistacia vera, has not been reported to date against breast cancer cells. Therefore, in this study, we aimed to assess the antitumor effect of these metabolites and investigate their potential as an anticancer agents. The cytotoxic activity of the secondary metabolites were evaluated by MTT analysis method, and the apoptotic activity in cells were evaluated by DNA fragmentation and caspase-3 enzyme expression by immunocytochemical analysis. Our results showed that masticadienonic acid and tricallol strongly reduced cell profileration of cancer cell lines. We found that these compounds induced apoptotic processes by enhancing caspase-3 expression and DNA fragmentation. These results suggest that it would be desirable to further investigate these compounds for their anti-breast cancer potential.Keywords: PistachioMasticadienonic acidTirucallolApoptosisAnti-breast cancer activityCytotoxicityDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.
{"title":"Evaluation of Antitumoral and Apoptotic Potentials of Masticadienonic Acid and Tirucallol from <i>Pistacia vera</i> L. Stems","authors":"Hatice Emlik, Isik Didem Karagoz, Lawali Yabo Dambagi, Basak Simitcioglu, Ahmet Cakir","doi":"10.1080/11263504.2023.2259386","DOIUrl":"https://doi.org/10.1080/11263504.2023.2259386","url":null,"abstract":"AbstractBreast cancer is one of the most common forms of cancer in women, therefore it has become necessary to find more effective and less toxic drugs in order to minimize the disadvantages of existing chemotherapeutic agents. The potential anticancer properties of masticadienonic acid, tirucallol and pistachionic acid, which are secondary metabolites isolated from Pistacia vera, has not been reported to date against breast cancer cells. Therefore, in this study, we aimed to assess the antitumor effect of these metabolites and investigate their potential as an anticancer agents. The cytotoxic activity of the secondary metabolites were evaluated by MTT analysis method, and the apoptotic activity in cells were evaluated by DNA fragmentation and caspase-3 enzyme expression by immunocytochemical analysis. Our results showed that masticadienonic acid and tricallol strongly reduced cell profileration of cancer cell lines. We found that these compounds induced apoptotic processes by enhancing caspase-3 expression and DNA fragmentation. These results suggest that it would be desirable to further investigate these compounds for their anti-breast cancer potential.Keywords: PistachioMasticadienonic acidTirucallolApoptosisAnti-breast cancer activityCytotoxicityDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134910915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-14DOI: 10.1080/11263504.2023.2259375
Apsara Kavital, M. B. Hiremath
AbstractThe research aimed to identify the bioactive compounds present in Syzygium cumini seeds by GC-MS and to determine the antioxidant, anti-inflammatory and antibacterial activities of seed extract. Phytochemical analysis revealed that phenols and flavonoids were more abundant in comparison to other compounds. The results of antioxidant activity as measured by DPPH assay and anti-inflammatory activity as measured by albumin denaturation assay revealed that the acetone extract exhibited significant antioxidant (inhibition % - 93.08 ± 0.43% and IC50 - 22.10 µg/mL) and anti-inflammatory activity (inhibition % 80.04 ± 0.52% and IC50 - 43.67 µg/mL). The extracts exhibited remarkable antibacterial activity with the zone of inhibition varying from 10.67 ± 0.75 to 16.67 ± 1.07 mm among which Staphylococcus aureus was more susceptible followed by Bacillus subtilis, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli and Klebsiella pneumoniae. GS-MS profiling revealed about 14 major bioactive compounds. S. cumini seed could be further utilized in pharmaceutical and food industries as a potent antioxidant, anti-inflammatory and antibacterial agent.Keywords: Anti-inflammatoryAntibacterialAntioxidantGC-MSPhytochemicalsSyzygium cuminiDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.
{"title":"Phytochemical screening and biological activities of <i>Syzygium cumini</i> seed extracts","authors":"Apsara Kavital, M. B. Hiremath","doi":"10.1080/11263504.2023.2259375","DOIUrl":"https://doi.org/10.1080/11263504.2023.2259375","url":null,"abstract":"AbstractThe research aimed to identify the bioactive compounds present in Syzygium cumini seeds by GC-MS and to determine the antioxidant, anti-inflammatory and antibacterial activities of seed extract. Phytochemical analysis revealed that phenols and flavonoids were more abundant in comparison to other compounds. The results of antioxidant activity as measured by DPPH assay and anti-inflammatory activity as measured by albumin denaturation assay revealed that the acetone extract exhibited significant antioxidant (inhibition % - 93.08 ± 0.43% and IC50 - 22.10 µg/mL) and anti-inflammatory activity (inhibition % 80.04 ± 0.52% and IC50 - 43.67 µg/mL). The extracts exhibited remarkable antibacterial activity with the zone of inhibition varying from 10.67 ± 0.75 to 16.67 ± 1.07 mm among which Staphylococcus aureus was more susceptible followed by Bacillus subtilis, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli and Klebsiella pneumoniae. GS-MS profiling revealed about 14 major bioactive compounds. S. cumini seed could be further utilized in pharmaceutical and food industries as a potent antioxidant, anti-inflammatory and antibacterial agent.Keywords: Anti-inflammatoryAntibacterialAntioxidantGC-MSPhytochemicalsSyzygium cuminiDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134910641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.1080/11263504.2023.2259372
Tatyana Sizonenko, Yuriy Dubrovskiy
Siberian larch (Larix sibirica) forests cover the largest areas in the Eurasian boreal zone, but the knowledge on its root system and ectomycorrhizas (EMs) are insufficient. The aim of this research is to find out whether the morphological parameters of L. sibirica EMs respond to the changes in altitude and main environmental factors. We measured the EM diameter, root diameter, mantle width and volume share, share of tannin cell layers, EM density and length of L. sibirica fine roots in four main types of habitats across the altitudinal gradient in the Urals. The diversity of fungal mantle types depended on the type of habitat. The minimal diversity of fungal mantles and the prevalence of plectenchymatous mantles were found in moister and unfertile habitats of sphagnous type. In other types of habitats, the diversity of fungal mantle types was approximately twice higher. A combination of factors (habitat type and altitude) significantly determined all quantitative parameters of the Larix EMs under study. We found a reduction in the EM linear dimensions with increasing altitude and a deterioration of environmental conditions, accompanied by a decrease in their functional parameters with a slight increase in the EM density and the share of nonmycorrhizal roots.
{"title":"Mycorrhizal parameters of <i>Larix sibirica</i> fine roots across gradients of main environmental factors and altitude","authors":"Tatyana Sizonenko, Yuriy Dubrovskiy","doi":"10.1080/11263504.2023.2259372","DOIUrl":"https://doi.org/10.1080/11263504.2023.2259372","url":null,"abstract":"Siberian larch (Larix sibirica) forests cover the largest areas in the Eurasian boreal zone, but the knowledge on its root system and ectomycorrhizas (EMs) are insufficient. The aim of this research is to find out whether the morphological parameters of L. sibirica EMs respond to the changes in altitude and main environmental factors. We measured the EM diameter, root diameter, mantle width and volume share, share of tannin cell layers, EM density and length of L. sibirica fine roots in four main types of habitats across the altitudinal gradient in the Urals. The diversity of fungal mantle types depended on the type of habitat. The minimal diversity of fungal mantles and the prevalence of plectenchymatous mantles were found in moister and unfertile habitats of sphagnous type. In other types of habitats, the diversity of fungal mantle types was approximately twice higher. A combination of factors (habitat type and altitude) significantly determined all quantitative parameters of the Larix EMs under study. We found a reduction in the EM linear dimensions with increasing altitude and a deterioration of environmental conditions, accompanied by a decrease in their functional parameters with a slight increase in the EM density and the share of nonmycorrhizal roots.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-11DOI: 10.1080/11263504.2023.2257703
Faisal K. Algethami, Mayssa Ben Mustapha, Mohamed R. Elamin, Babiker Y. Abdulkhair, Hichem Ben Jannet
{"title":"Biotransformation of Isocostic acid from <i>Dittrichia viscosa</i> Essential oil by <i>Saccharomyces cerevisiae</i>: <i>In vitro</i> Anti-tyrosinase Effect, Insights from Molecular Docking and Drug-Likeness Prediction","authors":"Faisal K. Algethami, Mayssa Ben Mustapha, Mohamed R. Elamin, Babiker Y. Abdulkhair, Hichem Ben Jannet","doi":"10.1080/11263504.2023.2257703","DOIUrl":"https://doi.org/10.1080/11263504.2023.2257703","url":null,"abstract":"","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-04DOI: 10.1080/11263504.2017.1338626
E. Del Guacchio, V. La Valva
Abstract We discuss several aspects of the alien flora of Campania (southern Italy), on the basis of an updated and annotated checklist. The non-native flora includes at present 346 taxa in 231 genera, representing 82 families. However, other 55 taxa were not found later than 1950. Trends in alien plants invasion throughout the investigated area are discussed. Results are therefore compared with similar data from other Italian regions and other European countries. In the Appendix, taxa are listed with essential synonymy, local and general distribution, status, biological form, habitat, diffusion mechanisms, potential threats, literature data, voucher specimens, and miscellaneous ethnobotanical, chronological and taxonomical notes. In addition, 49 escaped taxa are known only for Botanical and private gardens, while 66 taxa are regarded as doubtful aliens. By including also these two categories, the non-native flora of Campania reaches 516 taxa. In addition, numerous doubtful and erroneous indications were discussed. Finally, a reference list for the exotic regional flora from 1500 C.E. to present is proposed.
{"title":"The non-native vascular flora of Campania (southern Italy)","authors":"E. Del Guacchio, V. La Valva","doi":"10.1080/11263504.2017.1338626","DOIUrl":"https://doi.org/10.1080/11263504.2017.1338626","url":null,"abstract":"Abstract We discuss several aspects of the alien flora of Campania (southern Italy), on the basis of an updated and annotated checklist. The non-native flora includes at present 346 taxa in 231 genera, representing 82 families. However, other 55 taxa were not found later than 1950. Trends in alien plants invasion throughout the investigated area are discussed. Results are therefore compared with similar data from other Italian regions and other European countries. In the Appendix, taxa are listed with essential synonymy, local and general distribution, status, biological form, habitat, diffusion mechanisms, potential threats, literature data, voucher specimens, and miscellaneous ethnobotanical, chronological and taxonomical notes. In addition, 49 escaped taxa are known only for Botanical and private gardens, while 66 taxa are regarded as doubtful aliens. By including also these two categories, the non-native flora of Campania reaches 516 taxa. In addition, numerous doubtful and erroneous indications were discussed. Finally, a reference list for the exotic regional flora from 1500 C.E. to present is proposed.","PeriodicalId":54604,"journal":{"name":"Plant Biosystems","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2018-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/11263504.2017.1338626","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59769581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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