Pub Date : 2016-11-22DOI: 10.1186/s41065-016-0018-9
A. Genis-Mendoza, C. Tovilla-Zárate, Lilia López-Narváez, P. Mendoza-Lorenzo, P. Ostrosky-Wegman, H. Nicolini, T. González-Castro, Yazmín Hernández-Díaz
{"title":"Effect on the expression of drd2 and drd3 after neonatal lesion in the lymphocytes, nucleus accumbens, hippocampus and prefrontal cortex: comparative analysis between juvenile and adult Wistar rats","authors":"A. Genis-Mendoza, C. Tovilla-Zárate, Lilia López-Narváez, P. Mendoza-Lorenzo, P. Ostrosky-Wegman, H. Nicolini, T. González-Castro, Yazmín Hernández-Díaz","doi":"10.1186/s41065-016-0018-9","DOIUrl":"https://doi.org/10.1186/s41065-016-0018-9","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-016-0018-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-15DOI: 10.1186/s41065-016-0015-z
Hiroyo Kagami, K. Taguchi, Takumi Arakawa, Y. Kuroda, H. Tamagake, T. Kubo
{"title":"Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.)","authors":"Hiroyo Kagami, K. Taguchi, Takumi Arakawa, Y. Kuroda, H. Tamagake, T. Kubo","doi":"10.1186/s41065-016-0015-z","DOIUrl":"https://doi.org/10.1186/s41065-016-0015-z","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-016-0015-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-14DOI: 10.1186/s41065-016-0017-x
Chandler C. Roe, Kim Horn, E. Driebe, J. Bowers, J. Terriquez, P. Keim, D. Engelthaler
{"title":"Whole genome SNP typing to investigate methicillin-resistant Staphylococcus aureus carriage in a health-care provider as the source of multiple surgical site infections","authors":"Chandler C. Roe, Kim Horn, E. Driebe, J. Bowers, J. Terriquez, P. Keim, D. Engelthaler","doi":"10.1186/s41065-016-0017-x","DOIUrl":"https://doi.org/10.1186/s41065-016-0017-x","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-016-0017-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-23eCollection Date: 2016-01-01DOI: 10.1186/s41065-016-0014-0
Firuz Odilbekov, Eva Edin, Larisa Garkava-Gustavsson, Helena Persson Hovmalm, Erland Liljeroth
Background: Early blight, caused by the fungus Alternaria solani, occurs on potato mainly in the south-eastern part of Sweden, but also in other parts of the country. The aim of this study was to investigate the genetic diversity of A. solani populations from different potato growing regions in south-eastern Sweden using AFLP marker analysis. In addition, the cultured isolates were examined for substitutions in the gene encoding cytochrome b, associated with loss of sensitivity against QoI fungicides.
Results: Nei's gene diversity index for the Swedish populations of A. solani revealed a gene diversity of up to 0.20. Also genetic differentiation was observed among populations of A. solani from different locations in south-eastern Sweden. The mitochondrial genotype of the isolates of A. solani was determined and both known genotypes, GI (genotype 1) and GII (genotype 2), were found among the isolates. The occurrence of the F129L substitution associated with a loss of sensitivity to strobilurins was confirmed among the GII isolates. In vitro conidial germination tests verified that isolates containing the F129L substitution had reduced sensitivity to azoxystrobin and, at a lower extent, to pyraclostrobin.
Conclusions: Genetic diversity was relatively high among isolates of A. solani in south-eastern part of Sweden. F129L substitutions, leading to reduced sensitivity to strobilurins, have been established in field populations, which may have implications for the future efficacy of QoI fungicides.
背景：由Alternaria solani真菌引起的马铃薯早疫病主要发生在瑞典的东南部地区，但也发生在瑞典的其他地区。本研究的目的是利用 AFLP 标记分析法研究来自瑞典东南部不同马铃薯种植区的 A. solani 群体的遗传多样性。此外，还检测了培养分离物中编码细胞色素 b 的基因是否发生了替代，这与对 QoI 杀菌剂丧失敏感性有关：瑞典种群的内氏基因多样性指数（Nei's gene diversity index）显示基因多样性高达 0.20。瑞典东南部不同地区的 A. solani 种群之间也存在基因差异。对 A. solani 分离物的线粒体基因型进行了测定，在分离物中发现了两种已知的基因型：GI（基因型 1）和 GII（基因型 2）。在 GII 型分离物中证实了 F129L 替代的发生，它对石硫合剂失去了敏感性。体外分生孢子萌发试验证实，含有 F129L 替代的分离株对唑菌酰胺的敏感性降低，对吡唑醚菌酯的敏感性也有所降低：结论：瑞典东南部的茄属真菌分离物的遗传多样性相对较高。在田间种群中已经发现了 F129L 替代，这种替代会导致对石硫合剂的敏感性降低，这可能会对 QoI 杀菌剂的未来药效产生影响。
{"title":"Genetic diversity and occurrence of the F129L substitutions among isolates of <i>Alternaria solani</i> in south-eastern Sweden.","authors":"Firuz Odilbekov, Eva Edin, Larisa Garkava-Gustavsson, Helena Persson Hovmalm, Erland Liljeroth","doi":"10.1186/s41065-016-0014-0","DOIUrl":"10.1186/s41065-016-0014-0","url":null,"abstract":"<p><strong>Background: </strong>Early blight, caused by the fungus <i>Alternaria solani,</i> occurs on potato mainly in the south-eastern part of Sweden, but also in other parts of the country. The aim of this study was to investigate the genetic diversity of <i>A. solani</i> populations from different potato growing regions in south-eastern Sweden using AFLP marker analysis. In addition, the cultured isolates were examined for substitutions in the gene encoding cytochrome <i>b</i>, associated with loss of sensitivity against QoI fungicides.</p><p><strong>Results: </strong>Nei's gene diversity index for the Swedish populations of <i>A. solani</i> revealed a gene diversity of up to 0.20. Also genetic differentiation was observed among populations of <i>A. solani</i> from different locations in south-eastern Sweden. The mitochondrial genotype of the isolates of <i>A. solani</i> was determined and both known genotypes, GI (genotype 1) and GII (genotype 2), were found among the isolates. The occurrence of the F129L substitution associated with a loss of sensitivity to strobilurins was confirmed among the GII isolates. In vitro conidial germination tests verified that isolates containing the F129L substitution had reduced sensitivity to azoxystrobin and, at a lower extent, to pyraclostrobin.</p><p><strong>Conclusions: </strong>Genetic diversity was relatively high among isolates of <i>A. solani</i> in south-eastern part of Sweden. F129L substitutions, leading to reduced sensitivity to strobilurins, have been established in field populations, which may have implications for the future efficacy of QoI fungicides.</p>","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In animal cells, inhibition of non-homologous end joining (NHEJ) pathway improves the efficiency of homologous recombination (HR)-mediated double-strand brakes (DSBs) repair. To improve the efficiency of HR in sheep embryo fibroblasts, the NHEJ key molecule DNA ligase 4 (Lig4) was suppressed by siRNA interference. Four pairs of siRNA targeting Lig4 were designed and chemically synthesized. These siRNA were electro-transferred into sheep embryo fibroblasts respectively. Compared with the control groups, two pairs of siRNA were identified to effectively inhibit the expression of sheep Lig4 gene by qRT-PCR and Western blotting. The plasmid rejoining assay was adopted for examining the efficiency of HR-mediated DSB repair. I-SceⅠ endonuclease linearized vector and siRNA were co-transfected into sheep embryo fibroblasts. Flow cytometry analysis of cells after transfection for 72 h showed that suppression of Lig4 using siRNAs increased the rejoining efficiency of HR vector by 3-4 times compared with the control groups. Therefore, enhanced HR vector rejoining frequency by instant inhabition of Lig4 gene provides theoretical basis for improving gene targeting efficiency of sheep embryo fibroblasts.
{"title":"Increasing the efficiency of homologous recombination vector-mediated end joining repair by inhibition of Lig4 gene using siRNA in sheep embryo fibroblasts.","authors":"Wei Wang, Yu-shuang Wang, Lan-lan Huang, Zi-jian Jian, Xin-Hua Wang, Shou-ren Liu, Wen-hui Pi","doi":"10.16288/j.yczz.16-074","DOIUrl":"https://doi.org/10.16288/j.yczz.16-074","url":null,"abstract":"In animal cells, inhibition of non-homologous end joining (NHEJ) pathway improves the efficiency of homologous recombination (HR)-mediated double-strand brakes (DSBs) repair. To improve the efficiency of HR in sheep embryo fibroblasts, the NHEJ key molecule DNA ligase 4 (Lig4) was suppressed by siRNA interference. Four pairs of siRNA targeting Lig4 were designed and chemically synthesized. These siRNA were electro-transferred into sheep embryo fibroblasts respectively. Compared with the control groups, two pairs of siRNA were identified to effectively inhibit the expression of sheep Lig4 gene by qRT-PCR and Western blotting. The plasmid rejoining assay was adopted for examining the efficiency of HR-mediated DSB repair. I-SceⅠ endonuclease linearized vector and siRNA were co-transfected into sheep embryo fibroblasts. Flow cytometry analysis of cells after transfection for 72 h showed that suppression of Lig4 using siRNAs increased the rejoining efficiency of HR vector by 3-4 times compared with the control groups. Therefore, enhanced HR vector rejoining frequency by instant inhabition of Lig4 gene provides theoretical basis for improving gene targeting efficiency of sheep embryo fibroblasts.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In 2015, there are significant progresses in many aspects of the microbial genetics in China. To showcase the contribution of Chinese scientists in microbial genetics, this review surveys several notable progresses in microbial genetics made largely by Chinese scientists, and some key findings are highlighted. For the basic microbial genetics, the components, structures and functions of many macromolecule complexes involved in gene expression regulation have been elucidated. Moreover, the molecular basis underlying the recognition of foreign nucleic acids by microbial immune systems was unveiled. We also illustrated the biosynthetic pathways and regulators of multiple microbial compounds, novel enzyme reactions, and new mechanisms regulating microbial gene expression. And new findings were obtained in the microbial development, evolution and population genetics. For the industrial microbiology, more understanding on the molecular basis of the microbial factory has been gained. For the pathogenic microbiology, the genetic circuits of several pathogens were depicted, and significant progresses were achieved for understanding the pathogen-host interaction and revealing the genetic mechanisms underlying antimicrobial resistance, emerging pathogens and environmental microorganisms at the genomic level. In future, the genetic diversity of microbes can be used to obtain specific products, while gut microbiome is gathering momentum.
{"title":"Research advances on microbial genetics in China in 2015.","authors":"X. Jianping, H. Yubo, L. Gang, B. Linquan","doi":"10.16288/J.YCZZ.16-305","DOIUrl":"https://doi.org/10.16288/J.YCZZ.16-305","url":null,"abstract":"In 2015, there are significant progresses in many aspects of the microbial genetics in China. To showcase the contribution of Chinese scientists in microbial genetics, this review surveys several notable progresses in microbial genetics made largely by Chinese scientists, and some key findings are highlighted. For the basic microbial genetics, the components, structures and functions of many macromolecule complexes involved in gene expression regulation have been elucidated. Moreover, the molecular basis underlying the recognition of foreign nucleic acids by microbial immune systems was unveiled. We also illustrated the biosynthetic pathways and regulators of multiple microbial compounds, novel enzyme reactions, and new mechanisms regulating microbial gene expression. And new findings were obtained in the microbial development, evolution and population genetics. For the industrial microbiology, more understanding on the molecular basis of the microbial factory has been gained. For the pathogenic microbiology, the genetic circuits of several pathogens were depicted, and significant progresses were achieved for understanding the pathogen-host interaction and revealing the genetic mechanisms underlying antimicrobial resistance, emerging pathogens and environmental microorganisms at the genomic level. In future, the genetic diversity of microbes can be used to obtain specific products, while gut microbiome is gathering momentum.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.16288/J.YCZZ.16-305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-08-11DOI: 10.1186/s41065-016-0013-1
Kaixin Fu, Zhihui Guo, Xinquan Zhang, Yan Fan, Wendan Wu, Daxu Li, Yan Peng, Linkai Huang, Ming-Mao Sun, S. Bai, Xiao Ma
{"title":"Insight into the genetic variability analysis and cultivar identification of tall fescue by using SSR markers","authors":"Kaixin Fu, Zhihui Guo, Xinquan Zhang, Yan Fan, Wendan Wu, Daxu Li, Yan Peng, Linkai Huang, Ming-Mao Sun, S. Bai, Xiao Ma","doi":"10.1186/s41065-016-0013-1","DOIUrl":"https://doi.org/10.1186/s41065-016-0013-1","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-016-0013-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-06DOI: 10.1186/s41065-016-0010-4
Fang Liu, Tengfei Shi, Sisi Huang, Linsheng Yu, S. Bi
{"title":"Genetic structure of Mount Huang honey bee (Apis cerana) populations: evidence from microsatellite polymorphism","authors":"Fang Liu, Tengfei Shi, Sisi Huang, Linsheng Yu, S. Bi","doi":"10.1186/s41065-016-0010-4","DOIUrl":"https://doi.org/10.1186/s41065-016-0010-4","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-016-0010-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-01DOI: 10.1186/s41065-016-0011-3
M. Ghasemkhani, L. Garkava-Gustavsson, E. Liljeroth, H. Nybom
{"title":"Assessment of diversity and genetic relationships of Neonectria ditissima: the causal agent of fruit tree canker","authors":"M. Ghasemkhani, L. Garkava-Gustavsson, E. Liljeroth, H. Nybom","doi":"10.1186/s41065-016-0011-3","DOIUrl":"https://doi.org/10.1186/s41065-016-0011-3","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-016-0011-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-30eCollection Date: 2016-01-01DOI: 10.1186/s41065-016-0012-2
Fang Huang, Jiawei Shen, Qingli Guo, Yongyong Shi
Background: Enhancers are tissue specific distal regulation elements, playing vital roles in gene regulation and expression. The prediction and identification of enhancers are important but challenging issues for bioinformatics studies. Existing computational methods, mostly single classifiers, can only predict the transcriptional coactivator EP300 based enhancers and show low generalization performance.
Results: We built a hybrid classifier called eRFSVM in this study, using random forests as a base classifier, and support vector machines as a main classifier. eRFSVM integrated two components as eRFSVM-ENCODE and eRFSVM-FANTOM5 with diverse features and labels. The base classifier trained datasets from a single tissue or cell with random forests. The main classifier made the final decision by support vector machines algorithm, with the predicting results of base classifiers as inputs. For eRFSVM-ENCODE, we trained datasets from cell lines including Gm12878, Hep, H1-hesc and Huvec, using ChIP-Seq datasets as features and EP300 based enhancers as labels. We tested eRFSVM-ENCODE on K562 dataset, and resulted in a predicting precision of 83.69 %, which was much better than existing classifiers. For eRFSVM-FANTOM5, with enhancers identified by RNA in FANTOM5 project as labels, the precision, recall, F-score and accuracy were 86.17 %, 36.06 %, 50.84 % and 93.38 % using eRFSVM, increasing 23.24 % (69.92 %), 97.05 % (18.30 %), 76.90 % (28.74 %), 4.69 % (89.20 %) than the existing algorithm, respectively.
Conclusions: All these results demonstrated that eRFSVM was a better classifier in predicting both EP300 based and FAMTOM5 RNAs based enhancers.
{"title":"eRFSVM: a hybrid classifier to predict enhancers-integrating random forests with support vector machines.","authors":"Fang Huang, Jiawei Shen, Qingli Guo, Yongyong Shi","doi":"10.1186/s41065-016-0012-2","DOIUrl":"10.1186/s41065-016-0012-2","url":null,"abstract":"<p><strong>Background: </strong>Enhancers are tissue specific distal regulation elements, playing vital roles in gene regulation and expression. The prediction and identification of enhancers are important but challenging issues for bioinformatics studies. Existing computational methods, mostly single classifiers, can only predict the transcriptional coactivator EP300 based enhancers and show low generalization performance.</p><p><strong>Results: </strong>We built a hybrid classifier called eRFSVM in this study, using random forests as a base classifier, and support vector machines as a main classifier. eRFSVM integrated two components as eRFSVM-ENCODE and eRFSVM-FANTOM5 with diverse features and labels. The base classifier trained datasets from a single tissue or cell with random forests. The main classifier made the final decision by support vector machines algorithm, with the predicting results of base classifiers as inputs. For eRFSVM-ENCODE, we trained datasets from cell lines including Gm12878, Hep, H1-hesc and Huvec, using ChIP-Seq datasets as features and EP300 based enhancers as labels. We tested eRFSVM-ENCODE on K562 dataset, and resulted in a predicting precision of 83.69 %, which was much better than existing classifiers. For eRFSVM-FANTOM5, with enhancers identified by RNA in FANTOM5 project as labels, the precision, recall, F-score and accuracy were 86.17 %, 36.06 %, 50.84 % and 93.38 % using eRFSVM, increasing 23.24 % (69.92 %), 97.05 % (18.30 %), 76.90 % (28.74 %), 4.69 % (89.20 %) than the existing algorithm, respectively.</p><p><strong>Conclusions: </strong>All these results demonstrated that eRFSVM was a better classifier in predicting both EP300 based and FAMTOM5 RNAs based enhancers.</p>","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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