Pub Date : 2026-03-17DOI: 10.1186/s12964-026-02799-y
Diego Salagre, Marina Villalón-Mir, Jennifer Rieusset, Ahmad Agil
{"title":"Melatonin promotes skeletal muscle mitochondria-associated endoplasmic reticulum membrane contacts and restores organellar membrane integrity and phospholipid composition in Zücker diabetic fatty rats of both sexes.","authors":"Diego Salagre, Marina Villalón-Mir, Jennifer Rieusset, Ahmad Agil","doi":"10.1186/s12964-026-02799-y","DOIUrl":"https://doi.org/10.1186/s12964-026-02799-y","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147470320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1186/s12964-026-02796-1
Małgorzata Grzanka, Piotr Popławski, Jacek R Wiśniewski, Roksana Iwanicka-Nowicka, Helena Kossowska, Marta Koblowska, Beata Rybicka, Alex Białas, Agnieszka Piekiełko-Witkowska
{"title":"TCOF1 affects Golgi secretory pathway contributing to the angiogenesis in renal cancer.","authors":"Małgorzata Grzanka, Piotr Popławski, Jacek R Wiśniewski, Roksana Iwanicka-Nowicka, Helena Kossowska, Marta Koblowska, Beata Rybicka, Alex Białas, Agnieszka Piekiełko-Witkowska","doi":"10.1186/s12964-026-02796-1","DOIUrl":"https://doi.org/10.1186/s12964-026-02796-1","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147470328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Integration of the hepatitis B virus (HBV) genome into the host chromosome of infected patients poses a threat to those with HBV-associated hepatocellular carcinoma (HBV-HCC) due to challenges in early diagnosis and poor prognosis. CircRNAs are known for their oncogenic and biomarker potential in various cancers, including HBV-HCC, by sequestering tumor suppressive miRNAs, which, when free, can silence the expression of oncogenic mRNAs. Therefore, we aimed to develop a bioinformatic model to identify the circRNA-miRNA-mRNA axis in HBV-integrated HCC cell lines and to identify prognostic biomarkers specific to HBV-HCC patients.
Methods: We identified dysregulated host circRNAs and mRNAs in HBV-negative and HBV-integrated cells using RNA-seq, followed by differential gene expression analysis with DESeq, and performed pathway analysis using Gene Set Enrichment Analysis (GSEA). Junctional sequences of the circRNAs were validated by Sanger sequencing of the amplified products. RT-qPCR further confirmed the dysregulation of 9 randomly selected circRNAs chosen from those with the highest fold-change and adjusted p-values. The miRNA partners for each circRNA were identified using mirDB. miRNA expression validation was performed using the publicly available Gene Expression Omnibus (GEO) database of the same cells, and Empirical Cumulative Distribution Function (ECDF) plots were generated to assess the fold change of mRNAs in potential binding miRNA partners. The mRNA targets for 10 miRNA ECDF plots were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and hub genes were identified using Search Tool for the Retrieval of Interacting Genes (STRING) Cytohubba protein-protein interaction (PPI) analysis. Survival analysis of hub genes was plotted, and a competitive endogenous RNA (ceRNA) network was constructed using Cytoscape.
Results: We identified 494 dysregulated circRNAs, 346 dysregulated miRNAs, and 10,419 dysregulated mRNA in HBV-integrated cells through a comprehensive bioinformatic model. circADGRL2 (~ 25-fold) showed the highest upregulation and miR-361-5p acted as a central node of multiple circRNAs: circADGRL2, circPROX1 and circPALS2. BDNF, a target mRNA of miR-361-5p, was identified as the highest risk ratio in HBV-HCC patients, suggesting a possible circADGRL2-miR-361-5p-BDNF axis involved in HBV-HCC. The target mRNAs of miRNAs were predicted to be associated with several cancer pathways, such as MAPK and RAS.
Conclusion: Our data suggest a potential dysregulated circRNA-miRNA-mRNA axis in HBV-integrated hepatocytes, which may indicate a poor prognosis for HBV-HCC patients.
背景：乙型肝炎病毒（HBV）基因组整合到感染患者的宿主染色体对HBV相关肝细胞癌（HBV- hcc）患者构成威胁，因为早期诊断面临挑战，预后不良。众所周知，CircRNAs在包括HBV-HCC在内的各种癌症中具有致癌和生物标志物潜力，通过隔离肿瘤抑制mirna，这些mirna在游离时可以沉默致癌mrna的表达。因此，我们旨在建立一种生物信息学模型来鉴定hbv整合HCC细胞系中的circRNA-miRNA-mRNA轴，并鉴定HBV-HCC患者特异性的预后生物标志物。方法：利用RNA-seq技术在hbv阴性细胞和hbv整合细胞中鉴定出异常的宿主环状rna和mrna，随后利用DESeq进行差异基因表达分析，并利用基因集富集分析（gene Set Enrichment analysis， GSEA）进行途径分析。对扩增产物进行Sanger测序，验证环状rna的连接序列。RT-qPCR进一步证实了随机选择的9个circrna的失调，这些circrna是从折叠变化和调整后的p值最高的circrna中选择的。使用mirDB鉴定每个circRNA的miRNA伴侣。使用相同细胞的公开基因表达综合数据库（GEO）进行miRNA表达验证，并生成经验累积分布函数（ECDF）图来评估潜在结合miRNA伴侣的mrna的折叠变化。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）途径分析10个miRNA ECDF图的mRNA靶点，并使用Search Tool for Retrieval of Interacting Genes （STRING）分析Cytohubba protein-protein interaction (PPI)，鉴定枢纽基因。绘制了枢纽基因的生存分析图，并利用Cytoscape构建了竞争性内源RNA （ceRNA）网络。结果：通过综合生物信息学模型，我们在hbv整合细胞中鉴定出494个异常的环状rna， 346个异常的mirna和10419个异常的mRNA。circADGRL2（约25倍）表现出最高的上调，miR-361-5p作为多个circrna的中心节点：circADGRL2， circPROX1和circPALS2。BDNF是miR-361-5p的靶mRNA，在HBV-HCC患者中风险比最高，提示circadgrl1 -miR-361-5p-BDNF轴可能参与HBV-HCC。mirna的靶mrna被预测与几种癌症通路相关，如MAPK和RAS。结论：我们的数据表明hbv整合肝细胞中可能存在circRNA-miRNA-mRNA轴异常，这可能预示着HBV-HCC患者预后不良。
{"title":"Decoding the circRNA-miRNA-mRNA regulatory network in hepatitis B virus-driven hepatocellular carcinoma.","authors":"Kainat Ahmed, Anwaruddin Mohammad, Nan Chaiyariti, Danya Sankaranarayanan, Pankaj Kumar, Sudhakar Jha","doi":"10.1186/s12964-026-02812-4","DOIUrl":"https://doi.org/10.1186/s12964-026-02812-4","url":null,"abstract":"<p><strong>Background: </strong>Integration of the hepatitis B virus (HBV) genome into the host chromosome of infected patients poses a threat to those with HBV-associated hepatocellular carcinoma (HBV-HCC) due to challenges in early diagnosis and poor prognosis. CircRNAs are known for their oncogenic and biomarker potential in various cancers, including HBV-HCC, by sequestering tumor suppressive miRNAs, which, when free, can silence the expression of oncogenic mRNAs. Therefore, we aimed to develop a bioinformatic model to identify the circRNA-miRNA-mRNA axis in HBV-integrated HCC cell lines and to identify prognostic biomarkers specific to HBV-HCC patients.</p><p><strong>Methods: </strong>We identified dysregulated host circRNAs and mRNAs in HBV-negative and HBV-integrated cells using RNA-seq, followed by differential gene expression analysis with DESeq, and performed pathway analysis using Gene Set Enrichment Analysis (GSEA). Junctional sequences of the circRNAs were validated by Sanger sequencing of the amplified products. RT-qPCR further confirmed the dysregulation of 9 randomly selected circRNAs chosen from those with the highest fold-change and adjusted p-values. The miRNA partners for each circRNA were identified using mirDB. miRNA expression validation was performed using the publicly available Gene Expression Omnibus (GEO) database of the same cells, and Empirical Cumulative Distribution Function (ECDF) plots were generated to assess the fold change of mRNAs in potential binding miRNA partners. The mRNA targets for 10 miRNA ECDF plots were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and hub genes were identified using Search Tool for the Retrieval of Interacting Genes (STRING) Cytohubba protein-protein interaction (PPI) analysis. Survival analysis of hub genes was plotted, and a competitive endogenous RNA (ceRNA) network was constructed using Cytoscape.</p><p><strong>Results: </strong>We identified 494 dysregulated circRNAs, 346 dysregulated miRNAs, and 10,419 dysregulated mRNA in HBV-integrated cells through a comprehensive bioinformatic model. circADGRL2 (~ 25-fold) showed the highest upregulation and miR-361-5p acted as a central node of multiple circRNAs: circADGRL2, circPROX1 and circPALS2. BDNF, a target mRNA of miR-361-5p, was identified as the highest risk ratio in HBV-HCC patients, suggesting a possible circADGRL2-miR-361-5p-BDNF axis involved in HBV-HCC. The target mRNAs of miRNAs were predicted to be associated with several cancer pathways, such as MAPK and RAS.</p><p><strong>Conclusion: </strong>Our data suggest a potential dysregulated circRNA-miRNA-mRNA axis in HBV-integrated hepatocytes, which may indicate a poor prognosis for HBV-HCC patients.</p>","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147476304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1186/s12964-026-02806-2
Lindsay H Burns, Alex Romero
{"title":"Filamin A phosphorylation at S2152: A molecular switch fueling cancer and neurodegeneration.","authors":"Lindsay H Burns, Alex Romero","doi":"10.1186/s12964-026-02806-2","DOIUrl":"https://doi.org/10.1186/s12964-026-02806-2","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147470341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-14DOI: 10.1186/s12964-026-02817-z
Xinyu Zhang, Jiahao Liu, Kai Mao, Jingsheng Xu, Xiangyin Qian, Yao Rong, Tian Tian, Kai Wei, Minjie Chu, Yan Zhang
{"title":"Orchestrating homolog segregation in meiosis I: molecular logic and regulatory networks with emphasis on male metaphase I.","authors":"Xinyu Zhang, Jiahao Liu, Kai Mao, Jingsheng Xu, Xiangyin Qian, Yao Rong, Tian Tian, Kai Wei, Minjie Chu, Yan Zhang","doi":"10.1186/s12964-026-02817-z","DOIUrl":"https://doi.org/10.1186/s12964-026-02817-z","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-14DOI: 10.1186/s12964-026-02811-5
Thomas Duret, Mohammed Elmallah, Audrey Heraud-Meley, Cloé Tessier, Ana Valeria Vinhais Da Silva, Stéphanie Chadet, Roxane Lemoine, Justine Gainche, Valérie Labas, Ana-Paula Teixeira-Gomes, Daniel Tomas, Vesna Cuplov, Lin-Hua Jiang, Lili Fan, Gang Yin, Christophe Baron, Sébastien Roger
{"title":"The P2X4 purinergic receptor controls the autophagy-related release of small extracellular vesicles from mammary cancer cells under hypoxia.","authors":"Thomas Duret, Mohammed Elmallah, Audrey Heraud-Meley, Cloé Tessier, Ana Valeria Vinhais Da Silva, Stéphanie Chadet, Roxane Lemoine, Justine Gainche, Valérie Labas, Ana-Paula Teixeira-Gomes, Daniel Tomas, Vesna Cuplov, Lin-Hua Jiang, Lili Fan, Gang Yin, Christophe Baron, Sébastien Roger","doi":"10.1186/s12964-026-02811-5","DOIUrl":"https://doi.org/10.1186/s12964-026-02811-5","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-14DOI: 10.1186/s12964-026-02800-8
Zhiyao Ma, Haiming Lin, Daniel Young, Aahil Ansari, Melanie Kunze, Aillette Mulet-Sierra, Maria Febbraio, Daniel Graf, Antoine Dufour, Adetola B Adesida
{"title":"Simulated microgravity models sex differences in knee osteoarthritis through a CD36-regulated mechano-metabolic circuit.","authors":"Zhiyao Ma, Haiming Lin, Daniel Young, Aahil Ansari, Melanie Kunze, Aillette Mulet-Sierra, Maria Febbraio, Daniel Graf, Antoine Dufour, Adetola B Adesida","doi":"10.1186/s12964-026-02800-8","DOIUrl":"https://doi.org/10.1186/s12964-026-02800-8","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147460979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-14DOI: 10.1186/s12964-026-02810-6
Kewei Zhao, Dan Han, Qiuyue Wen, Pengye Li, Qiaoyun Tan, Lu Zhou, Liling Zhang
{"title":"Glutathione S-transferase inhibitor NBDHEX induces ROS-dependent immunogenic cell death and enhances PD-1 blockade in DLBCL.","authors":"Kewei Zhao, Dan Han, Qiuyue Wen, Pengye Li, Qiaoyun Tan, Lu Zhou, Liling Zhang","doi":"10.1186/s12964-026-02810-6","DOIUrl":"https://doi.org/10.1186/s12964-026-02810-6","url":null,"abstract":"","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147460938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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