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Investigation on the effects of Inula viscosa L. on rainbow trout gonad cells induced by lipopolysaccharide 粘菊对脂多糖诱导虹鳟鱼性腺细胞影响的研究
IF 0.7 Q4 FISHERIES Pub Date : 2021-04-13 DOI: 10.17017/j.fish.218
Azime Küçükgül, A. Küçükgül, Tülay Metin Korkmaz
Fish cells have been accepted as an alternative to in vivo assay for inflammatory effects of therapeutic experimental systems. To reveal the anti-inflammatory effects of Inula viscosa (IV) fish cell line RTG-2, derived from the gonadal tissue of rainbow trout, was infected with lipopolysaccharide (LPS). Effective concentrations for different concentrations of LPS (1, 5, 10, 20 µM) and IN (1, 5, 10, 20 µg ml–1) were determined. While the 20 µM concentration of LPS, which was effectively selected from preliminary tests, caused 27% cell loss,  the effective 1 µg ml–1 concentration of IN caused 1.1% proliferation in the cells when compared to the control group. All pro-inflammatory parameters investigated in LPS-induced RTG-2 cells showed up-regulation, with the highest increase in TNF-α gene expression level (11.3 fold changes). Down-regulation was determined in the IN together with LPS administered group and IL-1β had the highest effect with 96%. IL-6 protein level decreased by LPS at a rate of 4% and IN together with LPS increased by 9%. The IN exhibited significant efficacy against inflammation caused by LPS. However, further studies are needed to determine pharmacological activity of I. viscosa in details.
鱼细胞已被接受作为治疗实验系统的炎症效应的体内试验的替代方法。用脂多糖(LPS)感染虹鳟(Inula viscosa, IV)生殖腺组织细胞RTG-2,观察其抗炎作用。测定不同浓度LPS(1、5、10、20µM)和IN(1、5、10、20µg ml-1)的有效浓度。与对照组相比,20µM浓度的有效LPS使细胞损失27%,而1µg ml-1的有效IN使细胞增殖率为1.1%。lps诱导的RTG-2细胞所有促炎参数均出现上调,其中TNF-α基因表达水平升高最高(变化11.3倍)。in联合LPS组均有下调,其中IL-1β的下调率最高,达96%。LPS使IL-6蛋白水平降低了4%,而IN与LPS联合使IL-6蛋白水平升高了9%。IN对LPS引起的炎症有明显的抑制作用。但粘胶菌的药理活性还有待进一步研究。
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引用次数: 0
Quality changes in sea grape, Caulerpa lentillifera at different brine concentrations 不同盐水浓度下海葡萄、小扁豆的品质变化
IF 0.7 Q4 FISHERIES Pub Date : 2021-04-13 DOI: 10.17017/j.fish.278
P. D. H. Tolentino, J. Sorio
Sea grapes are among the seaweeds being commercialized in the Philippines. They are sold in fresh state, and are highly perishable. It is necessary to develop preservation techniques in order to lengthen its shelf life. This study aims to assess the physico-chemical, microbial and sensorial quality of sea grape Caulerpa lentillifera preserved in 0%, 5%, 10% and 15% brine concentrations. Results revealed that brining effectively extended the shelf life of C. lentillifera by inhibiting the growth of bacteria and maintaining the osmotic pressure between the product and the solution. Within the 10 days storage period, 10% and 15% were bacteriologically stable and more acceptable upon rehydration. Treatments 0% and 5% were not acceptable because it exceeded the bacterial limit set for fresh vegetables. The sensorial attributes were compromised and became less acceptable due to degradation through bacterial action. In terms of physico-chemical analysis, high salt concentration (15%) decreased chlorophyll and carotenoid content significantly due to shrinkage and water loss. Overall, this study proved that sea grapes in 10% brine solution can extend its shelf life.
海葡萄是菲律宾商业化的海藻之一。它们以新鲜状态出售,极易腐烂。为了延长其保质期,有必要开发保鲜技术。本研究旨在评价海葡萄在0%、5%、10%和15%盐水浓度下的理化、微生物和感官品质。结果表明,盐水通过抑制细菌生长和维持产品与溶液之间的渗透压,有效延长了香菇的保质期。在10天的储藏期内,10%和15%的水分在细菌学上是稳定的,在补液时更容易被接受。0%和5%的处理是不可接受的,因为它超过了新鲜蔬菜的细菌限制。由于细菌作用的降解,感觉属性受到损害,变得不太可接受。理化分析表明,高盐浓度(15%)显著降低了叶绿素和类胡萝卜素含量,主要是由于收缩和水分流失。总的来说,本研究证明,10%的盐水溶液可以延长海葡萄的保质期。
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引用次数: 1
Potential use of mud clam (Geloina coaxans) in producing sauce with papaya crude extraction as a protein hydrolysing agent 用木瓜粗提物作为蛋白质水解剂生产酱料的潜在用途
IF 0.7 Q4 FISHERIES Pub Date : 2021-03-17 DOI: 10.17017/j.fish.269
H. Wijerathna, K. Radampola, H. W. Cyril
Mud clam (Geloina coaxans) are underutilised food source due to the lack of consumer preference in Sri Lanka. Hence, this study was conducted to produce clam sauce as a value added product using the muscle of mud clams by means of accelerated fermentation method. Specimens were collected from Tambalagam Bay, Sri Lanka. Shell length, height, inflation, total weight with shell and without shell were 6.3 ± 0.4 cm, 5.3 ± 0.3 cm, 3.5 ± 0.3 cm, 83.1 ± 13.4 g and 14.9 ± 1.3 g respectively. The extracted mean meat yield was 14.9 ± 1.3% per mud clam. Moisture, crude protein, crude lipid and ash (dry weight) content of raw meat were 80.45 ± 0.89%, 64.14 ± 0.96%, 3.55 ± 0.39% and 7.54 ± 0.61% respectively. Final sauce product shows liquid yield, energy value, 0Brix value, pH, % NaCl, total nitrogen, moisture and ash content as 98.3 ± 5.5 ml 100g–1, 2124 ± 133 J g–1, 24.3 ± 0.9%, 5.02 ± 0.04, 14.53 ± 0.27%, 0.27 ± 0.01%, 74.06 ± 0.56% and 19.66 ± 1.99% respectively. The study concluded that the mud clam meat is a possible candidate as a raw material for the production of clam sauce.
由于斯里兰卡缺乏消费者偏好,泥蛤(Geloina coaxans)是未充分利用的食物来源。为此,本研究以蛤肉为原料,采用加速发酵法生产蛤酱作为一种增值产品。标本采集于斯里兰卡的Tambalagam湾。壳长6.3±0.4 cm,高5.3±0.3 cm,充气率3.5±0.3 cm,总重83.1±13.4 g,无壳总重14.9±1.3 g。提取的平均肉产率为14.9±1.3%。生肉的水分、粗蛋白质、粗脂肪和灰分(干重)含量分别为80.45±0.89%、64.14±0.96%、3.55±0.39%和7.54±0.61%。最终酱料的产液率、能量值、白度值、pH、% NaCl、总氮、水分和灰分含量分别为98.3±5.5 ml 100g-1、2124±133 J - 1、24.3±0.9%、5.02±0.04、14.53±0.27%、0.27±0.01%、74.06±0.56%和19.66±1.99%。研究结果表明,泥蛤肉可作为生产蛤酱的原料。
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引用次数: 0
Growth dependent changes in protein efficiency and amino acid composition in cultured meagre Argyrosomus regius 培养的瘦弱方鸡蛋白质效率和氨基酸组成的生长依赖性变化
IF 0.7 Q4 FISHERIES Pub Date : 2021-02-23 DOI: 10.17017/j.fish.274
B. Baki, D. Öztürk, M. Kerim
The goal of this research was to determine the growth dependent changes in protein efficiency and amino acid composition of the cultured meagre Argyrosomus regius (Asso, 1801). The research was carried in net cages in a private company (Gokce Off-shore Sist.) in the Aegean Sea. Meagre of an initial weight of 8.22 ± 0.13 g reached to 373.96 ± 15.65 g in 420 days of production period. Average protein efficiency rate, protein store rate and protein consumption values of cultured meagre were 1.05 ± 0.11%, 11.49 ± 2.98% and 56.67 ± 14.52 g respectively. The total amino acid values varied between 14.97 ± 0.14 and 18.87 ± 0.10 g 100g–1, the statistical difference among the study periods was significant (p < 0.05). Most of the total essential (EAA), non-essential (NEAA), and branched‐chain amino acid values of cultured meagre were decreasing with increasing fish size but EAA/NEAA ratios were increasing. The findings of this study showed that the composition of amino acids and the consistency of meagre culture were adequate and appropriate for human consumption and nutrition.
本研究的目的是确定培养的瘦弱的阿吉索蝇(Argyrosomus regius)的蛋白质效率和氨基酸组成的生长依赖性变化(Asso, 1801)。这项研究是在爱琴海一家私人公司(Gokce offshore Sist.)的网箱中进行的。最初的体重为8.22±0.13 g,在420天的生产期间达到了373.96±15.65 g。培养菌的平均蛋白质效率、蛋白质储存率和蛋白质消耗值分别为1.05±0.11%、11.49±2.98%和56.67±14.52 g。总氨基酸值分别为14.97±0.14和18.87±0.10 g 100g-1,各研究期间差异有统计学意义(p < 0.05)。鱼体总必需氨基酸(EAA)、非必需氨基酸(NEAA)和支链氨基酸值随着鱼体大小的增加而降低,但EAA/NEAA比值呈增加趋势。本研究结果表明,氨基酸的组成和微量培养物的稠度是足够的,适合人类食用和营养。
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引用次数: 0
Length–weight relationship and condition factor of ten cyprinid fish species from the Caspian Sea, Urmia Lake and Persian Gulf basins of Iran 伊朗里海、乌尔米亚湖和波斯湾盆地10种鲤科鱼类的长重关系及条件因子
IF 0.7 Q4 FISHERIES Pub Date : 2021-02-12 DOI: 10.17017/j.fish.268
A. Mouludi-Saleh, S. Eagderi, K. Abbasi, S. Salavatian
In this study, the length-weight relationships (LWRs) and condition factor (K) were estimated for 1334 specimens belonging to ten species including Acanthobrama marmid, A. microlepis, A. urmianus, Romanogobio persus, Leuciscus aspius, Luciobarbus capito, L. mursa, L. caspius, Alburnus atropatenae and Petroleuciscus ulanus from the Caspian Sea, Urmia Lake and Persian Gulf basins of Iran. The total length and weight of the individuals varied from 3.4 to 84.5 cm and 0.4 to 6600 g respectively. The values of the slope parameter (b) and condition factor ranged from 2.99 – 3.38 and 0.79 – 1.25 respectively. LWRs with high correlation coefficients were calculated for all species (R2 > 0.95). Based on the results, the allometric growth pattern of the studied species were positive except for L. aspius, L. capito, L. mursa and P. ulanus. This study provides useful data regarding the LWRs and condition factor of ten fish species in Iranian inland waters that can be used in fisheries management, stock assessment and future studies.
本文对来自伊朗里海、乌尔米湖和波斯湾盆地的10个物种1334个标本进行了长度-权重关系(LWRs)和条件因子(K)的估算。这些物种包括棘鱼、小鳞棘鱼、乌尔米亚棘鱼、波斯棘鱼、白斑棘鱼、白斑棘鱼、白斑棘鱼、白斑棘鱼、白斑棘鱼、白斑棘鱼、白斑棘鱼和乌尔米亚棘鱼。个体总长3.4 ~ 84.5 cm,总重0.4 ~ 6600 g。坡度参数(b)和条件因子的取值范围分别为2.99 ~ 3.38和0.79 ~ 1.25。所有物种的lwr均具有较高的相关系数(R2 > 0.95)。结果表明,除羊草、羊草、羊草和羊草外,其余树种均呈异速生长阳性。这项研究提供了关于伊朗内陆水域10种鱼类的最低水限和状况因子的有用数据,可用于渔业管理、种群评估和今后的研究。
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引用次数: 2
Ichthyofauna of Gibe Sheleko National Park and Some Morphometric Relationships of Fish of the Tributary Rivers, Southern Ethiopia 埃塞俄比亚南部Gibe Sheleko国家公园鱼类区系与支流河流鱼类的形态关系
IF 0.7 Q4 FISHERIES Pub Date : 2021-02-10 DOI: 10.21203/RS.3.RS-157750/V1
S. Mekonen, Abera Hailu
This study was carried out along a tributary of Gibe Sheleko National Park to investigate the diversity, relative abundance and morphometric characteristics of fish. By using monofilament gill-nets, fish were collected, their species identifiedand their morphometric data measured. A total of 10 species were identified, dominated overall by Synodontis schall (28.52%), followed by Labeobarbus nedgia (18.88%) and Labeobarbus intermedius (14.06%). According to the Index of Relative Importance (IRI), S. schall was also first (49.13%), followed by L. intermedius (15.49%). There were no significance differences in the IRI values of fish and relative abundance between the dry and wet season (p > 0.05). Synodontis schall had the largest girth (23.1 cm), but Clarias gariepinus had the largest eye diameter (8.4 mm) and body weight (1713.4 g). Heterobranchus longifilis was first in total fork and standard length (64.7 cm, 64.7 cm and 59.8 cm, respectively). However, the paired t test of the total body length was not significantly different from the fork length (Pn> 0.05), but not showing statistical differences from standard length, weight and girth (P < 0.05). Gibe River had a higher number of species (N) than Wabe River in both seasons. The Shannon Diversity Index (H') in Gibe River was also higher (2.09) than Wabe (1.84) during the dry season, but lowest in the wet season (1.52 and 1.57, respectively). Furthermore, both (N) and (H') were not statistically different between tributaries and seasons (p > 0.05). Generally, differences in sampling habitats and fishing effort might have contributed to discovered variants findings. Fishery development should be implemented in the Park to use the fish resource sustainably.
本研究沿着Gibe Sheleko国家公园的一条支流进行,以调查鱼类的多样性、相对丰度和形态特征。采用单丝刺网采集鱼类,鉴定鱼种,测定形态特征。共鉴定出10种,总体上以小唇唇虫(Synodontis schall)为主,占28.52%,其次为negia Labeobarbus negia,占18.88%,中间Labeobarbus intermedius,占14.06%。相对重要性指数(IRI)显示,沙蝇(49.13%)居首,中蝇(15.49%)次之。鱼的IRI值和相对丰度在干湿季节间无显著差异(p < 0.05)。长尾异枝(Heterobranchus longifilis)的总叉长和标准长分别为64.7 cm、64.7 cm和59.8 cm,长尾异枝(Clarias gariepinus)最大,眼直径为8.4 mm,体重为1713.4 g。但经配对t检验,总体长与叉长差异无统计学意义(Pn> 0.05),与标准体长、体重、周长差异无统计学意义(P < 0.05)。吉布河在两个季节的种数(N)均高于瓦贝河。干季吉贝河的香农多样性指数(H’)为2.09,高于Wabe河(1.84),湿季最低,分别为1.52和1.57。此外,(N)和(H’)在支流和季节之间差异无统计学意义(p < 0.05)。一般来说,取样栖息地和捕鱼努力的差异可能导致了发现的变异结果。在园区内实施渔业开发,实现鱼类资源的可持续利用。
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引用次数: 0
Effects of feeding frequency on the growth and production performance of indigenous catfish Clarias batrachus (Linnaeus, 1758) in ponds of Northern Bangladesh 投喂频率对孟加拉北部池塘本地鲶鱼claras batrachus (Linnaeus, 1758)生长和生产性能的影响
IF 0.7 Q4 FISHERIES Pub Date : 2021-01-30 DOI: 10.17017/j.fish.302
Md. Selim Reza, M. Samad, J. Ferdous
Effects of feeding frequency on the growth performance and production of Clarias batrachus was evaluated for a period of 180 days. Feeding frequency were once (T1), twice (T2) and thrice (T3) per day. Stocking density was 16750 fingerlings ha–1. The fish were initially fed 28% protein containing formulated feed at the rate of 8% of body weight which subsequently reduced to 5% at the end. The water quality parameters were monitored fortnightly and found similar across treatments and remained within the suitable ranges for aquaculture. The mean weight gain was 41.14±0.15, 44.49±0.78 and 56.10±0.86 g and specific growth rate (SGR) of 1.29±0.002, 1.33±0.01 and 1.44±0.01% per day in T1, T2 and T3 respectively. The highest survival rate was in T2 (96.23±0.76%) whereas the lowest was in T1 (95.32±0.76%). The lowest FCR (=best; 1.65±0.12) was found in T3 and the highest FCR (=worst; 2.14±0.15) was recorded in T1. The highest production was observed in T3 (3559.27±85.95 kg ha–1), which was significantly higher than that of other treatments. This study concludes that feeding three times per day is effective for C. batrachus.
以180 d为试验期,研究了不同投喂频率对batrachus生长性能和产量的影响。饲喂频率分别为每天1次(T1)、2次(T2)和3次(T3)。放养密度为16750尾/ h。试验初期以体重的8%饲喂含28%蛋白质的配方饲料,后期降至5%。每两周监测一次水质参数,发现不同处理的水质参数相似,并保持在适合水产养殖的范围内。T1、T2和T3的平均日增重分别为41.14±0.15、44.49±0.78和56.10±0.86 g,特定生长率(SGR)分别为1.29±0.002、1.33±0.01和1.44±0.01%。T2生存率最高(96.23±0.76%),T1生存率最低(95.32±0.76%)。最低FCR(=最佳;T3为1.65±0.12),FCR最高(=最差;T1为2.14±0.15)。T3处理产量最高(3559.27±85.95 kg ha-1),显著高于其他处理。本研究结果表明,每天饲喂三次是有效的。
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引用次数: 2
Water pollution effects of river Saraswati and Kunti on livelihood: a case study of fishermen communities in Tribeni and Kuntighat, Hooghly, West Bengal Saraswati河和Kunti河的水污染对生计的影响:以西孟加拉邦Hooghly的Tribeni和Kuntighat渔民社区为例
IF 0.7 Q4 FISHERIES Pub Date : 2020-12-24 DOI: 10.17017/j.fish.293
A. Panigrahi, S. Pattnaik
A prolific study was conducted to find out the impact of pollution on the socio-economic status of the fishermen community of Tribeni, Kuntighat - Hooghly, West Bengal, who depend on the rivers Saraswati, Kunti and Ganga for their livelihood. Analysis of important water quality parameters clearly reflects the pollution status of the rivers Saraswati and Kunti. Investigation was carried out by a previously tested questionnaire and personal interaction with 100 fishermen based on which the probable reasons of decline in fish diversity were drawn, which consequently adversely affected the economic conditions of fishermen. The investigation revealed a positive correlation between discharge of industrial effluents, in turn depleting the availability of fish food organisms and fish diversity and warning socioeconomic conditions of fishermen. Results clearly indicate proper steps should be taken by the policy makers towards restoration of water quality and thereby helping the vulnerable fishermen.
一项多产的研究旨在找出污染对西孟加拉邦孔蒂哈特-胡格利特里贝尼渔民社区社会经济地位的影响,他们依靠萨拉斯瓦蒂河、孔蒂河和恒河谋生。对重要水质参数的分析清楚地反映了萨拉斯瓦蒂河和昆蒂河的污染状况。调查是通过先前测试过的问卷和与100名渔民的个人互动进行的,在此基础上得出鱼类多样性下降的可能原因,从而对渔民的经济状况产生不利影响。调查显示,工业废水排放之间存在正相关关系,这反过来又消耗了鱼类食物生物和鱼类多样性的供应,并警告了渔民的社会经济状况。结果清楚地表明,决策者应采取适当措施恢复水质,从而帮助脆弱的渔民。
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引用次数: 2
Effect of carp species combination on production and economics of stinging catfish, Heteropneustes fossilis based polyculture in homestead ponds under drought prone area of Bangladesh 孟加拉国干旱易发地区鲤种组合对以刺鲶、异鳞鱼化石为基础的家庭池塘混养产量和经济的影响
IF 0.7 Q4 FISHERIES Pub Date : 2020-12-23 DOI: 10.17017/j.fish.282
S. Nabi, Md Akhtar Hossain, M. M. Alam, M. Harun-ur-Rashid, Md. Anwar Hossain
Aquaculture of homestead ponds under drought prone areas is climatically challenged by increased temperature and decreased water level. These ponds are potentials for farming of a short cycle aquaculture species like Heteropneustes fossilis with carps but lack of appropriate species combination is the major constraint for promotion of this technique. To address this problem, an experiment was carried out during July to December, 2019 to evaluate the production and economics of H. fossilis based polyculture under different carp species combinations in homestead ponds in Tanore of Rajshahi district, Bangladesh. Three different combination of carps were tested under three treatments as T1:  Labeo rohita dominant carps (L. rohita 50%, Gibelion catla 25% and Hypophthalmichthys molitrix 25%); T2: G. catla dominant carps (L. rohita 25%, G. catla 50% and H. molitrix 25%); and T3: H. molitrix dominant carps (L. rohita 25%, G. catla 25% and H. molitrix 50%), each with three replications. Stocking densities were 123500 ha–1 for of H. fossilis was and 988 ha–1 for carps. Water quality was monitored monthly and found within acceptable range. T3 was found significantly (p < 0.05) better than other groups in terms of yield and cost benefit ratio.
在干旱易发地区,温度升高和水位下降对养殖场养殖构成了气候挑战。这些池塘具有与鲤鱼一起养殖短周期水产养殖物种的潜力,但缺乏适当的物种组合是该技术推广的主要制约因素。为了解决这一问题,于2019年7月至12月在孟加拉国Rajshahi地区Tanore的家庭池塘中进行了一项实验,以评估不同鲤鱼物种组合下基于H.化石的混养产量和经济性。3种不同组合的鲤鱼在3种处理下进行试验,T1:罗氏Labeo rohita优势鲤鱼(罗氏L. rohita占50%,Gibelion catla占25%,Hypophthalmichthys molitrix占25%);T2:优势鲤(罗氏鲤25%,卡特拉鲤50%,莫里特鲤25%);T3为优势鲤(rohita 25%、G. catla 25%、H. molitrix 50%),每组3个重复。石首鱼和鲤鱼的放养密度分别为123500 ha-1和988 ha-1。每月监测水质,发现水质在可接受范围内。在产量和成本效益比方面,T3组显著(p < 0.05)优于其他各组。
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引用次数: 5
Does fish price depend solely on weight? A market survey analysis from four districts of West Bengal, India 鱼的价格完全取决于重量吗?印度西孟加拉邦四个地区的市场调查分析
IF 0.7 Q4 FISHERIES Pub Date : 2020-12-14 DOI: 10.17017/j.fish.263
Debjit Das, Soumalya Mukhopadhyay, Adity Sarbajna, S. Chakraborty
Fish is an important food item that contains perfect combination of essential nutrients. The main consumable fishes in West Bengal, India are various carps and other small indigenous fishes. The objective of the study was to find out whether fish weight was a crucial factor to decide pricing and what kind of fish was beneficial for consumer from economic perspective. Thirteen markets in four districts (Kolkata, North and South 24 Parganas, Howrah) of West Bengal were surveyed to collect information about price, weight and number of vendors selling a particular fish considering 22 fish species. Subsequent statistical analyses were performed to conclude about the relationship between fish weight and price. The study indicates that pricing of every fish species does not depend solely on their weight. Weight plays important role to determine the price of few fish species such as Liza persia, Lates calcarifer, Wallago attu, Ompok pabo and Mystus tengara. But, the price of small indigenous fishes like Chanda nama, Colisa fasciata, Harpadon nehereus, Monopterus cuchia, Rhingomugil corsula, Sperata aor and Puntius puntio does not depend on the weight of individual fish. Seasonal availability, taste, consumer’s personal preference have important role to determine fish price.
鱼是一种重要的食物,它包含了必需营养素的完美组合。印度西孟加拉邦的主要消费鱼类是各种鲤鱼和其他小型本地鱼类。本研究的目的是从经济角度探讨鱼的重量是否是决定价格的关键因素,以及什么样的鱼对消费者有利。在西孟加拉邦的四个区(加尔各答,北部和南部24 Parganas, Howrah)的13个市场进行了调查,以收集有关价格,重量和销售特定鱼类的摊贩数量的信息,考虑到22种鱼类。随后进行统计分析,得出鱼重与价格之间的关系。研究表明,每种鱼类的价格并不仅仅取决于它们的重量。重量在决定一些鱼类的价格方面起着重要的作用,如丽扎波斯、拉特斯钙化鱼、瓦拉戈阿图、Ompok pabo和米斯图斯滕加拉。但是,像Chanda nama、Colisa fasciata、Harpadon nehereus、Monopterus cuchia、Rhingomugil corsula、Sperata aor和Puntius puntio这样的小型本地鱼类的价格并不取决于单鱼的重量。季节性供应、口味、消费者个人偏好对鱼的价格有重要影响。
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引用次数: 2
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Journal of Fisheries
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