Journal of Applied Animal Welfare Science最新文献

Laboratory fish play an important role in many research fields, and they are also raised and bred in many laboratories around the world. While many studies have suggested suitable feeding densities for various laboratory fish, significant variations exist in the sizes of rearing tanks across different laboratories. Moreover, there is limited research on the effects of different rearing tank sizes. In this study, we set up the same rearing density (one liter of water corresponding to two fishes) and four treatments with different tank sizes (5 L, 10 L, 15 L, 20 L). We found that different sizes of rearing tanks had no significant effect on the growth and shoaling of laboratory rare minnow. However, the rare minnow in 10 L tank had lower cortisol levels, while the rare minnow in 20 L tank had higher levels of dopamine (DA), serotonin (5-HT) and their metabolites (DOPAC and 5-HIAA). These results show that even under the same rearing density, different sizes of rearing tanks will still have a physiological effect on the rare minnow, and the tank size suitable for laboratory fish should be considered.

Civet coffee is produced with coffee beans that have passed through the digestive tract of civets which are then processed for drinking. Outlets, known as civet coffee tourism plantations are popular tourist attractions in Bali, Indonesia. These outlets keep caged civets for public viewing and sell civet coffee. We assessed civet welfare in 29 civet coffee tourism plantations to assess husbandry factors, the five freedoms and body condition scores. We also assessed civet welfare in eight wildlife markets where civets are sourced for civet coffee tourism and calculated turnover of civets. We found 99 civets in civet coffee tourism plantations and 136 in markets, where. Annually between 400 and 800 civets are sold. Welfare standards do not meet the Indonesian laws and regulations. Diet, hygiene, presence of water and mobility affected body condition scores and no civet met all of the five freedoms. Enforcement of welfare and trade laws is needed to improve civet welfare, and to curb the illegal civet trade. Tourists frequenting civet coffee tourism plantations should be made aware of the low welfare standards to inform their choice about supporting this industry.

Sex determination in monomorphic birds is a precondition for captive breeding programs and management and conservation strategies for threatened species. Most species of the order Psittaciformes often present complications since these birds lack external sexual phenotypic traits, making it impossible to differentiate males and females. In the present study, we used molecular techniques to determine the sex of 31 individuals belonging to nine species of the order Psittaciformes kept under human care at the Akumal Monkey Sanctuary & Rescued Animals in Quintana Roo, Mexico. This is a useful and low-cost methodology based on the analysis of the conserved region of the CHD1 gene, which was amplified by PCR with two sets of primers: P8/P2 and 2550F/2718 R. All individuals were successfully sexed with the first set of primers, while only 28 out of 31 samples (90%) could be amplified with the second set. Out of the 31 individuals analyzed, fifteen are female, and seventeen are male. This information represents a handy tool for adequately managing birds under human care, resulting in their reproduction and eventual reintegration into their natural habitat.

The way horses are trained has implications for equine welfare and training success, yet little is known about the factors that influence horse-owners' choice of training approach (TA). Limited understanding in this area will hinder the development and dissemination of evidence-based training advice to owners. Consequently, this study aims to identify demographic and attitudinal factors that influence horse-owner TA selection. A 22-question online survey collected information from 1,593 horse-owners about their demographics, equestrian activities, goals and beliefs. Participants rated how likely they were to use six different horse TAs on a five-point scale. Multinomial regression analysis and Spearman's correlation coefficients were used to identify factors associated with their likelihood of using each TA. Several factors were associated with reported TA use, including age, gender identity, goals, activities, industry role and whether they had training in animal behavior. Beliefs about equine sentience, cognitive ability and whether science should inform horse training correlated with likelihood of applying aversives. This study provides insight for further research and development of educational strategies to reduce the use of training approaches that may compromise equine welfare.

We propose a practical method of assessing the personality of horses using five personality axes. Four evaluators empirically judged 19 horse individuals on specific adjectives for each axis. To validate the questionnaire, four behavioral tests were conducted with these same animals (social interactions, novel object test, bridge test,and arena test). In this tests, the frequency of specific behaviors were evaluated to create a scale related to the same personality adjectives and judge the animals based on their reactions.The questionnaire was reliable in determining the personality of horses, since the results were consistent with those obtained through behavioral tests. Additionally, in this group of horses attention reactions were more frequent than fear reactions, but significant differences occurred among tests. This study proposes a practical questionnaire for owners and trainers to assess the personality of their horses. The application of this tool can improve the relationship between humans and horses, directing a more empathic approach in the everyday routine with these animals.

Killing of farmed saltwater crocodiles involve stunning with a penetrative captive bolt device, followed by a cut across the nape of the neck and physical destruction of the brain to ensure death. This study was a welfare-based assessment of the use of a penetrative captive bolt device in saltwater crocodiles, to determine whether it satisfies the criteria of a humane stunning method and can be regarded as a direct killing method without the need for the application of an adjunct method. Methods used were electroencephalogram (EEG), observation of post-stun behavior, and postmortem examination of gross pathology of the cranium and brain. 30 of 30 animals, demonstrated immediate and irreversible loss of consciousness. There was extensive damage to the brain in all animals, deemed to be inconsistent with cortical function and possible recovery. The CASH Special 0.22 penetrative captive bolt pistol (1.25 grain cartridge), applied to the top of the cranial plate, produced immediate and irreversible unconsciousness in all the animals studied. This method satisfies animal welfare expectations, providing crocodile processors with a technique that contributes to a humane killing process.

Volunteers that provide foster care in their homes are a critical resource for animal shelters and rescues and make significant contributions to animal welfare. This project explores the support needs identified by canine foster volunteers via a national survey of 611 respondents by answering the following three research questions:1. Why do foster volunteers consider ending their foster service?2. What types of training and support do dog fosters want and need from their organizations?3. Do the types of support needed vary based on the nature of the dog fostered (those with medical versus behavioral challenges)?Based on descriptive, content, and correlation analysis the findings suggest that foster volunteers are generally satisfied with the support provided by their organizations. However, they are less positive about the extent of communication between volunteers and their organization, the amount of training they have received beyond the basics, and the social supports that would accrue from a foster volunteer network, areas that extant research suggests are important for volunteer satisfaction and retention.

The aquaculture industry frequently employs various slaughter methods that subject fish to inhumane conditions, resulting in significant suffering. This study examined the effects of electrical method on the welfare of farmed fish (Mesopotamichthys sharpeyi) compared to air asphyxiation. Fish captured with electricity exhibited calm behavior until death, in contrast to fish treated with air asphyxiation, which exhibited violent responses within 4 minutes of capture. The electrical method achieved a complete state of unconsciousness 7 minutes faster than air asphyxiation. Our results show that air asphyxiation raised cortisol levels more than the electrical method, with no significant difference in glucose and lactate concentrations. Electrically treated fish had higher superoxide dismutase and liver-reduced glutathione levels, while suffocated fish showed elevated GSH and liver catalase levels. Radiographs revealed no fractures or skeletal changes. Electrical stunning had no effect on gill tissue, but caused brain tissue hemorrhage, whereas air asphyxiation caused less damage. Air asphyxiation caused gill tissue issues but less brain damage. Consciousness loss is crucial for humane practices. Specific electrical currents (110V for 30s) could improve aquaculture and fish welfare.

Assessing quality of life in animals is an art as much as a science. Despite the use of questionnaires and keeper reports which consider several aspects of well-being, the process often remains subjective. Keepers have unique insights, and anecdotal observations can be enhanced with objective data. We combined the art and science of assessments in this study on a geriatric macaque (1.0 lion-tailed/pig-tailed (Macaca silenus/macaca nemestrina) hybrid), using historic data to inform management decisions. Following the unexpected death of his cage mate, his activity and engagement with keepers decreased, and new concerning behaviors presented. While the zoo worked to identify new social opportunities, we used these data to develop a plan to improve his quality of life (e.g., increase training sessions, enrichment, social interactions). After intense implementation, we saw a significant increase in activity level and engagement with keepers; the frequency of unexpected behaviors suggesting a lower quality of life, however, increased over time. Our data allowed us to objectively compare changes in behavior, enabling the zoo to make the most informed animal management decision possible. [Figure: see text].

Social tolerance is an essential feature of social life that can determine the good functioning of a group of animals. Play behaviors, like social play and playing with objects, are frequently associated with positive emotional and welfare states. As a result, zoos use various strategies to promote both social tolerance and play with objects. Providing animals with cognitive environmental enrichment can be an effective tool to achieve these goals. Here we tested whether cognitive environmental enrichment can promote social tolerance and play with objects in bottlenose dolphins (Tursiops truncatus). To this end, we provided a group of five dolphins with two types of cognitive enrichment: one for individual use and one for cooperative use, both based on the rope-pulling task paradigm. Then we evaluated whether social tolerance and play with objects had increased after we provided dolphins with the two enrichments. Our results go in this direction, showing that after we provided dolphins with the enrichments, their intolerance behaviors decreased, both during feeding sessions and play sessions, while their play with objects increased. As a result, the two enrichments we used could be useful for improving dolphins' housing conditions.