Pub Date : 2016-06-01DOI: 10.1016/S1006-8104(16)30049-6
Liu Chai , Li Qiang, Liu Cheng-hai, Zheng Xian-zhe
In order to study the microwave-assisted foam-mat drying properties of corn soaking water and optimize process parameters, a quadratic regression orthogonal rotary method was used to analyze the influence of microwave power, material weight, material thickness and drying time on moisture content (dry basis), color value and protein content. Results showed that the primary and secondary sequence of parameters with regard to moisture content (d. b.) was drying time, microwave power, material weight and material thickness; the primary and secondary sequence of parameters with regard to color value was material weight, drying time, microwave power and material thickness; the primary and secondary sequence of parameters with regard to protein content was drying time, material weight, microwave power and material thickness. Optimum conditions were obtained as microwave power of 560 W, material weight of 46.88 g, material thickness of 6.20 mm and drying time of 8.01 min. The results might provide the theoretical basis and technical support for the microwave-assisted foam-mat drying of corn soaking water to produce yeast protein power.
{"title":"Process Parameter Study on Microwave-assisted Foam-mat Drying Properties of Corn Soaking Water","authors":"Liu Chai , Li Qiang, Liu Cheng-hai, Zheng Xian-zhe","doi":"10.1016/S1006-8104(16)30049-6","DOIUrl":"10.1016/S1006-8104(16)30049-6","url":null,"abstract":"<div><p>In order to study the microwave-assisted foam-mat drying properties of corn soaking water and optimize process parameters, a quadratic regression orthogonal rotary method was used to analyze the influence of microwave power, material weight, material thickness and drying time on moisture content (dry basis), color value and protein content. Results showed that the primary and secondary sequence of parameters with regard to moisture content (<em>d. b.</em>) was drying time, microwave power, material weight and material thickness; the primary and secondary sequence of parameters with regard to color value was material weight, drying time, microwave power and material thickness; the primary and secondary sequence of parameters with regard to protein content was drying time, material weight, microwave power and material thickness. Optimum conditions were obtained as microwave power of 560 W, material weight of 46.88 g, material thickness of 6.20 mm and drying time of 8.01 min. The results might provide the theoretical basis and technical support for the microwave-assisted foam-mat drying of corn soaking water to produce yeast protein power.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30049-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-01DOI: 10.1016/S1006-8104(16)30045-9
Zaib-un Nisa , Chen Chen , Yang Yu , Chao Chen , ALi Inayat Mallano , Duan Xiang-bo , Sun Xiao-li , Zhu Yan-ming
The enzyme myo-inositol-1-phosphate synthase (MIPS EC 5.5.1.4) catalyzes the first step of myo-inositol biosynthesis, a product that plays crucial roles in plants as an osmoprotectant, transduction molecule, cell wall constituent and production of stress related molecule. Previous reports highlighted an important role of MIPS family genes in abiotic stresses particularly under salt stress tolerance in several plant species; however, little is known about the cellular and physiological functions of MIPS2 genes under abiotic conditions. In this study, a novel salt stress responsive gene designated GsMIPS2 from wild soybean Glycine soja 07256 was functionally characterized contained an open reading frame (ORF) of 1 533 bp coding a peptide sequence of 510 amino acids along with mass of 56 445 ku. Multiple sequence alignment analysis revealed its 92%-99% similarity with other MIPS family members in legume proteins. Quantitative real-time PCR results demonstrated that GsMIPS2 was induced by salt stress and expressed in roots of soybean. The positive function of GsMIPS2 under salt response at different growth stages of transgenic Arabidopsis was also elucidated. The results showed that GsMIPS2 transgenic lines displayed increased tolerance as compared to WT and atmips2 mutant lines under salt stress. Furthermore, the expression levels of some salt stress responsive marker genes, including KIN1, RD29A, RD29B, P5Cs and COR47 were significantly up-regulated in GsMIPS2 overexpression lines than wild type and atmips2 mutant. Collectively, these results suggested that GsMIPS2 gene was a positive regulator of plant tolerance to salt stress. This was the first report to demonstrate that overexpression of GsMIPS2 gene from wild soybean improved salt tolerance in transgenic Arabidopsis.
{"title":"Constitutive Overexpression of Myo-inositol-1-Phosphate Synthase Gene (GsMIPS2) from Glycine soja Confers Enhanced Salt Tolerance at Various Growth Stages in Arabidopsis","authors":"Zaib-un Nisa , Chen Chen , Yang Yu , Chao Chen , ALi Inayat Mallano , Duan Xiang-bo , Sun Xiao-li , Zhu Yan-ming","doi":"10.1016/S1006-8104(16)30045-9","DOIUrl":"10.1016/S1006-8104(16)30045-9","url":null,"abstract":"<div><p>The enzyme <em>myo</em>-inositol-1-phosphate synthase (MIPS EC 5.5.1.4) catalyzes the first step of <em>myo</em>-inositol biosynthesis, a product that plays crucial roles in plants as an osmoprotectant, transduction molecule, cell wall constituent and production of stress related molecule. Previous reports highlighted an important role of MIPS family genes in abiotic stresses particularly under salt stress tolerance in several plant species; however, little is known about the cellular and physiological functions of <em>MIPS</em>2 genes under abiotic conditions. In this study, a novel salt stress responsive gene designated <em>GsMIPS</em>2 from wild soybean <em>Glycine soja</em> 07256 was functionally characterized contained an open reading frame (ORF) of 1 533 bp coding a peptide sequence of 510 amino acids along with mass of 56 445 ku. Multiple sequence alignment analysis revealed its 92%-99% similarity with other MIPS family members in legume proteins. Quantitative real-time PCR results demonstrated that <em>GsMIPS</em>2 was induced by salt stress and expressed in roots of soybean. The positive function of <em>GsMIPS</em>2 under salt response at different growth stages of transgenic <em>Arabidopsis</em> was also elucidated. The results showed that <em>GsMIPS</em>2 transgenic lines displayed increased tolerance as compared to WT and <em>atmips</em>2 mutant lines under salt stress. Furthermore, the expression levels of some salt stress responsive marker genes, including <em>KIN</em>1<em>, RD</em>29<em>A, RD</em>29<em>B, P</em>5<em>Cs</em> and <em>COR</em>47 were significantly up-regulated in <em>GsMIPS</em>2 overexpression lines than wild type and <em>atmips</em>2 mutant. Collectively, these results suggested that <em>GsMIPS</em>2 gene was a positive regulator of plant tolerance to salt stress. This was the first report to demonstrate that overexpression of <em>GsMIPS</em>2 gene from wild soybean improved salt tolerance in transgenic <em>Arabidopsis.</em></p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30045-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-06-01DOI: 10.1016/S1006-8104(16)30046-0
Yuan Qiang , Wei Yun-min , Fu Ming-ming , Qiu You-wen , Wen Hong-tao , Zhang Ming-hui , Liu Ying , Ao Jin-xia
A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L−1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.
{"title":"Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean","authors":"Yuan Qiang , Wei Yun-min , Fu Ming-ming , Qiu You-wen , Wen Hong-tao , Zhang Ming-hui , Liu Ying , Ao Jin-xia","doi":"10.1016/S1006-8104(16)30046-0","DOIUrl":"10.1016/S1006-8104(16)30046-0","url":null,"abstract":"<div><p>A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L<sup>−1</sup>, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30046-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.1016/S1006-8104(16)30026-5
Ma Xiao-chi , Wei Wei , Ye Le-fu , Zhao Jia-nan , Zhao Kui-jun , Wang Zi-jian , Liu Xiao-jie , Fu Xue
This paper investigated effects of four ecological variables on the damage levels of elm leaf beetle (different effects of minimum and maximum values, relations between each two variables were also explored) and discussed current management measures. The results showed that among these factors, interval between elm trees significantly affected damage level by this leaf beetle species, and perimeter of elm trunk could change this effect. On the other side, while middle values for each index were deleted, effects comparison between minimum and maximum parts showed that good root soil as overwintering was related with more damage; more severe damages on elm tree would result in more control measures used; on elm tree with bigger trunk, more damages were observed; and if other elm tree was closer, more damages would occur. Secondly, good root soil was related with more measures used; good root soil was correlated with smaller perimeter; good root soil was associated with bigger interval. Thirdly, more measures were correlated with bigger perimeter; more measures were also correlated with good root soil. Fourthly, bigger perimeter was correlated with worse root soil. Current dominant control measures included chemical pesticide application preventing adult insects from descending or climbing up along trunk before and after overwintering. These results suggested that we should focus on elm leaf beetle management on all the elm trees instead of only on bigger elm trees at proper period simultaneously because occurrence on these elm trees were related intensively although this adult elm leaf beetle species could not fly and had limited movement ability.
{"title":"Studies on Occurrence Ecological Factors and Management Strategies of Ambrostoma quadriimpressum in Harbin City","authors":"Ma Xiao-chi , Wei Wei , Ye Le-fu , Zhao Jia-nan , Zhao Kui-jun , Wang Zi-jian , Liu Xiao-jie , Fu Xue","doi":"10.1016/S1006-8104(16)30026-5","DOIUrl":"10.1016/S1006-8104(16)30026-5","url":null,"abstract":"<div><p>This paper investigated effects of four ecological variables on the damage levels of elm leaf beetle (different effects of minimum and maximum values, relations between each two variables were also explored) and discussed current management measures. The results showed that among these factors, interval between elm trees significantly affected damage level by this leaf beetle species, and perimeter of elm trunk could change this effect. On the other side, while middle values for each index were deleted, effects comparison between minimum and maximum parts showed that good root soil as overwintering was related with more damage; more severe damages on elm tree would result in more control measures used; on elm tree with bigger trunk, more damages were observed; and if other elm tree was closer, more damages would occur. Secondly, good root soil was related with more measures used; good root soil was correlated with smaller perimeter; good root soil was associated with bigger interval. Thirdly, more measures were correlated with bigger perimeter; more measures were also correlated with good root soil. Fourthly, bigger perimeter was correlated with worse root soil. Current dominant control measures included chemical pesticide application preventing adult insects from descending or climbing up along trunk before and after overwintering. These results suggested that we should focus on elm leaf beetle management on all the elm trees instead of only on bigger elm trees at proper period simultaneously because occurrence on these elm trees were related intensively although this adult elm leaf beetle species could not fly and had limited movement ability.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30026-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.1016/S1006-8104(16)30027-7
Naveed Ahmed , Muhammad Razaq , Hasnain Alam , Salahuddin
This experiment was carried out at Mansehra during cropping season of 2013. There were three French bean cultivars and four different plant spacings. The experiment was laid out on a Randomized Complete Block Design (RCBD) with three replications. Different cultivars, plant spacings and their interactions significantly influenced all the parameters studied. Maximum days to flowering (59.33) and seed maturity (97.66) were recorded in cultivar Komal Green grown at 15 cm spacing, while, maximum 100-grain weight (42.20 g) was noted in cultivar Peshawar Local grown at 60 cm spacing. However, maximum fresh pod yield • plant−1 (109.67 g), number of seed • pod−1 (7.99) and seed yield • hm−2 (1 437.3 kg) were recorded in cultivar Paulista grown at spacing of 45 cm. Whereas, maximum plant height (40.50 cm) was noticed in cultivar Paulista grown at 15 cm plant spacing. While, the least number of days to flowering (50.33) and to seed maturity (85.66) were taken by cultivar Paulista grown at 60 cm plant spacing. Likewise, minimum seed yield (311.9 kg • hm−2) was recorded in plants of cultivar Komal Green spaced at 60 cm plant spacing. While, minimum fresh pod weight • plant−1 (67.00 g) and number of seed • pod−1 (4.66) were attained in cultivar Peshawar Local grown at 15 cm plant spacing. Whereas, minimum plant height (27.59 cm) and 100-grain weight (15.60 g) were recorded for cultivar Komal Green grown at 45 and 15 cm, respectively.
{"title":"Response of French Bean Cultivars to Plant Spacing Under Agroclimatic Condition of Baffa","authors":"Naveed Ahmed , Muhammad Razaq , Hasnain Alam , Salahuddin","doi":"10.1016/S1006-8104(16)30027-7","DOIUrl":"10.1016/S1006-8104(16)30027-7","url":null,"abstract":"<div><p>This experiment was carried out at Mansehra during cropping season of 2013. There were three French bean cultivars and four different plant spacings. The experiment was laid out on a Randomized Complete Block Design (RCBD) with three replications. Different cultivars, plant spacings and their interactions significantly influenced all the parameters studied. Maximum days to flowering (59.33) and seed maturity (97.66) were recorded in cultivar Komal Green grown at 15 cm spacing, while, maximum 100-grain weight (42.20 g) was noted in cultivar Peshawar Local grown at 60 cm spacing. However, maximum fresh pod yield • plant<sup>−1</sup> (109.67 g), number of seed • pod<sup>−1</sup> (7.99) and seed yield • hm<sup>−2</sup> (1 437.3 kg) were recorded in cultivar Paulista grown at spacing of 45 cm. Whereas, maximum plant height (40.50 cm) was noticed in cultivar Paulista grown at 15 cm plant spacing. While, the least number of days to flowering (50.33) and to seed maturity (85.66) were taken by cultivar Paulista grown at 60 cm plant spacing. Likewise, minimum seed yield (311.9 kg • hm<sup>−2</sup>) was recorded in plants of cultivar Komal Green spaced at 60 cm plant spacing. While, minimum fresh pod weight • plant<sup>−1</sup> (67.00 g) and number of seed • pod<sup>−1</sup> (4.66) were attained in cultivar Peshawar Local grown at 15 cm plant spacing. Whereas, minimum plant height (27.59 cm) and 100-grain weight (15.60 g) were recorded for cultivar Komal Green grown at 45 and 15 cm, respectively.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30027-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was conducted to see the short term impact of organic and inorganic fertilizers on soil microbial biomass both in spring and summer. Also aimed to observe the correlation between soil microbial biomass and soil DNA. The study concluded that type of fertilizer might alter the soil microbial biomass and DNA contents. In soil treated with organic fertilizers resulted in higher concentrations of microbial biomass and DNA contents in summer as compared to spring dute to increase in temperature. Correspondingly, in case of inorganic fertilizer, concentrations of soil microbial biomass and DNA detected higher in summer instead of spring. The statistical correlation between soil microbial biomass, DNA and ODR in spring and summer along with organic and inorganic fertilizers were calculated highly significant (p>0.01). This study demonstrated the impact of fertilizers and seasonal variations on soil microbial biomass and also revealed significant correlation between soil microbial biomass and soil DNA.
{"title":"Short Term Influence of Organic and Inorganic Fertilizer on Soil Microbial Biomass and DNA in Summer and Spring","authors":"Khalil ur Rehman, Zhang Ying, Shahla Andleeb, Zhao Jiang, Erinle Kehinda Olajide","doi":"10.1016/S1006-8104(16)30028-9","DOIUrl":"10.1016/S1006-8104(16)30028-9","url":null,"abstract":"<div><p>The present study was conducted to see the short term impact of organic and inorganic fertilizers on soil microbial biomass both in spring and summer. Also aimed to observe the correlation between soil microbial biomass and soil DNA. The study concluded that type of fertilizer might alter the soil microbial biomass and DNA contents. In soil treated with organic fertilizers resulted in higher concentrations of microbial biomass and DNA contents in summer as compared to spring dute to increase in temperature. Correspondingly, in case of inorganic fertilizer, concentrations of soil microbial biomass and DNA detected higher in summer instead of spring. The statistical correlation between soil microbial biomass, DNA and ODR in spring and summer along with organic and inorganic fertilizers were calculated highly significant (<em>p</em>>0.01). This study demonstrated the impact of fertilizers and seasonal variations on soil microbial biomass and also revealed significant correlation between soil microbial biomass and soil DNA.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30028-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.1016/S1006-8104(16)30032-0
Chen Dan, Zhang Na, Nan Xue-mei, Li Qing-zhang, Gao Xue-jun
The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways were involved in the regulation of milk protein synthesis in the dairy goat mammary gland. Total 36 primiparous Guanzhong dairy goats were allotted in 12 groups according to their mammary development stages: days 90 and 150 of virgin, days 30, 90, and 150 of pregnancy, days 1, 10, 35, and 60 of lactation and days 3, 7, and 21 of involution (three animals per group). Mammary tissue RNA was isolated for quantitative real-time RT-PCR of four casein genes alpha-s1 casein (CSN1S1), alpha-s2 casein (CSN1S2), beta-casein (CSN2) and casein kappa (CSN3), four whey protein genes lactoglobulin (LGB), lactalbumin (LALBA), lactofarrin (LTF), and Whey acidic protein (WAP) and the genes which were potentially to regulate dairy goat milk protein synthesis at the level of transcription or translation [prolactin receptor (PRLR), AKT1, signal transducers and activators of transcription 5 (STAT5), E74-Like Factor 5 (ELF5), eukaryotic translation initiation factor 4E binding protein 1 (EIF4E-BP1), S6kinase (S6K) and caveolin 1]. The results showed that all genes were up-regulated in lactation period. The expressions of PRLR, AKT1, STAT5, ELF5, and S6K were similar to mRNA expressions of milk proteins. Our results indicated that milk protein synthesis in dairy goat mammary gland was possibly regulated by these genes.
{"title":"Potential Genes for Regulation of Milk Protein Synthesis in Dairy Goat Mammary Gland","authors":"Chen Dan, Zhang Na, Nan Xue-mei, Li Qing-zhang, Gao Xue-jun","doi":"10.1016/S1006-8104(16)30032-0","DOIUrl":"10.1016/S1006-8104(16)30032-0","url":null,"abstract":"<div><p>The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways were involved in the regulation of milk protein synthesis in the dairy goat mammary gland. Total 36 primiparous Guanzhong dairy goats were allotted in 12 groups according to their mammary development stages: days 90 and 150 of virgin, days 30, 90, and 150 of pregnancy, days 1, 10, 35, and 60 of lactation and days 3, 7, and 21 of involution (three animals per group). Mammary tissue RNA was isolated for quantitative real-time RT-PCR of four casein genes alpha-s1 casein (CSN1S1), alpha-s2 casein (CSN1S2), beta-casein (CSN2) and casein kappa (CSN3), four whey protein genes lactoglobulin (LGB), lactalbumin (LALBA), lactofarrin (LTF), and Whey acidic protein (WAP) and the genes which were potentially to regulate dairy goat milk protein synthesis at the level of transcription or translation [prolactin receptor (PRLR), AKT1, signal transducers and activators of transcription 5 (STAT5), E74-Like Factor 5 (ELF5), eukaryotic translation initiation factor 4E binding protein 1 (EIF4E-BP1), S6kinase (S6K) and caveolin 1]. The results showed that all genes were up-regulated in lactation period. The expressions of PRLR, AKT1, STAT5, ELF5, and S6K were similar to mRNA expressions of milk proteins. Our results indicated that milk protein synthesis in dairy goat mammary gland was possibly regulated by these genes.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30032-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.1016/S1006-8104(16)30035-6
Ebewore Solomon Okeoghene, Achoja Felix Odemero
The study examined the popularity, role and economic viability of mini-livestock keeping in Delta State, Nigeria. The specific objectives were to describe the socio-economic characteristics of mini-livestock farmers, identify the types of animals reared as mini-livestock in the area, ascertain the reasons for keeping mini-livestock by farmers, determine the benefit level of mini-livestock farmers, and identify the problems facing mini-livestock farmers in the study area. Using snowball sampling technique, 674 respondents were sampled with the aid of a structured questionnaire. Various descriptive and inferential statistics were used for data analyses. The results of the study indicated various types of mini-livestock reared in the study area, mainly snail farming or heliciculture (about 90%) and rearing of cane rat (84.27%); the most important reasons for keeping mini-livestock were income (99.6%), employment (99.26%) and food (94.96%). The result further indicated that protein consumption and improved income were the most important benefits to respondents. In spite of these benefits, the respondents faced some constraints, amongst which were lack of technical information (X=4.70), lacking of enough space for expansion (X=4.58), inadequate training (X=4.20), poor extension delivery services (X=4.20) and problems of feed availability (X=3.86). In spite of these constraints, the production of mini-livestock was found to be profitable in the study area. Based on the findings, it was recommended, among others, that mini-livestock farmers in the study area should be trained on various aspects of the management of mini-livestock especially by organising workshops for them.
{"title":"Assessment of Economic Viability of Mini-livestock Production in Delta State, Nigeria: Implication for Extension Delivery Services","authors":"Ebewore Solomon Okeoghene, Achoja Felix Odemero","doi":"10.1016/S1006-8104(16)30035-6","DOIUrl":"10.1016/S1006-8104(16)30035-6","url":null,"abstract":"<div><p>The study examined the popularity, role and economic viability of mini-livestock keeping in Delta State, Nigeria. The specific objectives were to describe the socio-economic characteristics of mini-livestock farmers, identify the types of animals reared as mini-livestock in the area, ascertain the reasons for keeping mini-livestock by farmers, determine the benefit level of mini-livestock farmers, and identify the problems facing mini-livestock farmers in the study area. Using snowball sampling technique, 674 respondents were sampled with the aid of a structured questionnaire. Various descriptive and inferential statistics were used for data analyses. The results of the study indicated various types of mini-livestock reared in the study area, mainly snail farming or heliciculture (about 90%) and rearing of cane rat (84.27%); the most important reasons for keeping mini-livestock were income (99.6%), employment (99.26%) and food (94.96%). The result further indicated that protein consumption and improved income were the most important benefits to respondents. In spite of these benefits, the respondents faced some constraints, amongst which were lack of technical information (<em>X</em>=4.70), lacking of enough space for expansion (<em>X</em>=4.58), inadequate training (<em>X</em>=4.20), poor extension delivery services (<em>X</em>=4.20) and problems of feed availability (<em>X</em>=3.86). In spite of these constraints, the production of mini-livestock was found to be profitable in the study area. Based on the findings, it was recommended, among others, that mini-livestock farmers in the study area should be trained on various aspects of the management of mini-livestock especially by organising workshops for them.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30035-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56333397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.1016/S1006-8104(16)30037-X
Wu Jian-zhong
Flax is a kind of worldwide fiber and oil crops, and it has a very important role in economic crop production in the world. With the development of molecular biology techniques, the research of flax molecular level has a very big breakthrough. But, flax molecular biology researches are less reported due to the later starting. This paper summarized the latest research progress of molecular biology of flax, including molecular marker technology, construction of genetic map, gene engineering and omics researches, in order to provide the reference to understand the development and research status for flax molecular breeding researchers.
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Pub Date : 2016-03-01DOI: 10.1016/S1006-8104(16)30025-3
Lamine Diallo , Cao Qing-jun , Yang Zhen-ming , Cui Jin-hu , Teybelassma Ibrahim Mustafa Dafaalla
In Faranah Province (Guinea), the ratio of supply and demand of corn is approximately 1:2. The production is low and the deficit is covered by maize importation. This study was initiated in order to improve corn production by evaluating the effects of increasing doses of mineral fertilizers on the yield and economic profitability of corn. The experiment was conducted on a farm in Kamedankoude in a split plot with three replicates. Treatments included two varieties of maize (Perta and Kilissi113) and five doses of chemical fertilizers (D0=unfertilized control, D1=300 kg NPKS+200 kg urea • hm−2; D2=400 kg NPKS+800 kg urea • hm−2; D3= 200 kg NPKS+400 kg urea • hm−2 and D4=500 kg NPKS+600 kg urea • hm−2). The results showed that all the agronomic parameters increased with increasing dose of fertilizers, except for the number of ears per plant. The two varieties of corn tested produced similar yields. However, the profitability of mineral fertilizers decreased with increased doses and with value/cost ratio of 3. We recommended a mineral fertilizer dose of 300 kg NPKS + 200 kg urea. The high prices of mineral fertilizers were often barriers to intensive maize cultivation, especially for low-income farmers. This study was a contribution to rational fertilization and adapted the socio-economic conditions of the region, with the background knowledge that most farmers in the regions earned low incomes.
在法拉纳省(几内亚),玉米的供需比例约为1:2。产量很低,缺口由进口玉米弥补。本研究旨在通过评价增加矿肥用量对玉米产量和经济效益的影响,提高玉米产量。试验在Kamedankoude的一个农场进行,分为3个重复。处理包括2个玉米品种(Perta和Kilissi113)和5剂化肥(D0=未施肥对照,D1=300 kg NPKS+200 kg尿素•hm−2;D2=400 kg NPKS+800 kg尿素•hm−2;D3= 200kg NPKS+ 400kg尿素•hm−2,D4= 500kg NPKS+ 600kg尿素•hm−2)。结果表明,除单株穗数外,其余农艺参数均随施肥量的增加而增加。测试的两种玉米产量相似。然而,矿物肥料的盈利能力随着用量的增加而下降,价值/成本比为3。我们建议使用300公斤NPKS + 200公斤尿素的矿物肥料剂量。矿物肥料的高价格往往是玉米集约化种植的障碍,对低收入农民来说尤其如此。这项研究有助于合理施肥,适应该地区的社会经济条件,背景知识是该地区大多数农民收入较低。
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