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Phytochemical analysis, in vitro antioxidant and antibacterial activities of root extracts of Carduus macracanthus 马齿苋根提取物的植物化学分析、体外抗氧化和抗菌活性
Pub Date : 2017-10-21 DOI: 10.12980/JCLM.5.2017J7-110
M. Anza, Mesfin Bibiso, B. Alemayehu, Engeda Desalegn
Medicinal plants have played a pivotal role in the primary healthcare and formed the basis of traditional systems of medicines. They are effective in the treatment of infectious diseases while simultaneously mitigating many side effects that are often associated with synthetic antimicrobials. A disease that caused through pathogenic microorganisms is the major cause of deaths across the world. Increasing cases of drug resistance, unwanted side effects of existing antibiotics and the reappearance of earlier known infections lead to the need for new, safe and effective antimicrobial agents[1-3]. Thus, phytoconstituents such as phenolics are being considered to be safe and provide lesser chances for microbes to develop drug resistance. Oxidative stress is mainly caused by the reactive oxygen species (ROS) such as hydroxyl radical (·OH), peroxide (ROO·) and superoxide radicals (O2· ). These reactive oxygen species are classes of compounds formed from oxygen metabolism, and are able to cause severe damage to cells and tissues. The effect of oxidative stress is linked to cardiovascular diseases, cancer, inflammatory diseases and many other health issues. Antioxidants are significant agents in reducing oxidative stress which damages cells and biological molecules[4]. Natural antioxidants such as bioactive flavonoids are of great ARTICLE INFO ABSTRACT
药用植物在初级保健中发挥着关键作用,并构成了传统药物体系的基础。它们在治疗传染病方面是有效的,同时减轻了许多通常与合成抗菌药物有关的副作用。一种由病原微生物引起的疾病是世界各地死亡的主要原因。耐药性病例的增加、现有抗生素的不良副作用以及早期已知感染的再次出现,导致需要新的、安全有效的抗菌药物[1-3]。因此,酚类等植物成分被认为是安全的,并且为微生物产生耐药性提供的机会较小。氧化应激主要由活性氧(ROS)引起,如羟基自由基(·OH)、过氧化物(ROO·)和超氧化物自由基(O2·)。这些活性氧是由氧代谢形成的一类化合物,能够对细胞和组织造成严重损伤。氧化应激的影响与心血管疾病、癌症、炎症性疾病和许多其他健康问题有关。抗氧化剂是减少氧化应激的重要药物,氧化应激会损害细胞和生物分子[4]。天然抗氧化剂,如具有生物活性的黄酮类化合物是伟大的文章信息摘要
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引用次数: 4
Biodegradation of high density polyethylene using Streptomyces species 链霉菌对高密度聚乙烯的生物降解
Pub Date : 2017-10-21 DOI: 10.12980/JCLM.5.2017J7-94
A. Farzi, A. Dehnad, Najibeh Shirzad, Faezeh Norouzifard
Ali Farzi, Alireza Dehnad, Najibeh Shirzad, Faezeh Norouzifard Faculty of Chemical and Petroleum Engineering, University of Tabriz, Tabriz, Iran Biotechnology Department, East Azerbaijan Research and Education Center Agricultural and Natural Resources, AREEO, Tabriz, Iran Young Researchers and Elite Club, Ilkhchi Branch, Islamic Azad University, Ilkhchi, Iran Department of Chemical Engineering, Islamic Azad University, Tabriz Branch, Tabriz, Iran Journal of Coastal Life Medicine 2017; 5(11): 474-479
Ali Farzi, Alireza Dehnad, Najibeh Shirzad, Faezeh Norouzifard大不里士大学化学与石油工程学院,伊朗大不里士,生物技术系,东阿塞拜疆研究与教育中心,农业与自然资源,AREEO,大不里士,伊朗青年研究人员和精英俱乐部,伊朗伊斯兰阿扎德大学伊尔赫齐分校,伊朗伊斯兰阿扎德大学大不里士分校化学工程系,伊朗大不里士,伊朗大不里士,海岸生命医学杂志2017;5 (11): 474 - 479
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引用次数: 15
In vivo hypoglycemic, antinociceptive and in vitro antioxidant activities of methanolic bark extract of Crataeva nurvala 山楂树皮甲醇提取物体内降糖、抗伤及体外抗氧化活性的研究
Pub Date : 2017-10-21 DOI: 10.12980/jclm.5.2017J7-131
U. Jalal, Karim Samira, Gain Dwigbijoy, Hassan Mahmudul, Sultana Julie Sultana Julie Azima, K. Zubair
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引用次数: 0
Environmental effect of resort centres on the distribution of aquatic insect fauna in Ethiope River, Delta State, Nigeria 度假中心对尼日利亚三角洲州埃塞俄比亚河水生昆虫区系分布的环境影响
Pub Date : 2017-10-21 DOI: 10.12980/jclm.5.2017J7-130
E. E. Ito, I. L. Ugbomeh
Aquatic insects are macroinvertebrates that live on or associated with bottom of freshwater bodies[1]. Their pivotal role in nutrients circulation cannot be over emphasized in aquatic environment. Aquatic invertebrates are the link between unobtainable nutrients in detritus and protein materials in fishes[1,2]. Their role in fasttracking the breakdown of putrefying organic material into smaller inorganic forms such as nitrate and phosphate is also well documented[3]. These nutrients are usually utilized by aquatic plants in the ecosystem. Aquatic insects commonly used as biological indicator have been effective in monitoring environmental pollution and water quality[4,5]. The sensitivity and forbearance to environmental perturbation exhibited by aquatic insects vary considerably among orders and species. The composition and distribution of aquatic insect at any given point in a water course is a reflection of its water quality[5]. This makes aquatic insect a valuable tool in pollution and water quality assessment. Species richness of aquatic insects can be influenced by physicochemical parameters [temperature, dissolve oxygen (DO), biochemical oxygen demand (BOD) and turbidity] of water. The structural composition of insect communities has been the focus of many researches on aquatic system. The mere presence or absence of an individual of a species provides little information of ARTICLE INFO ABSTRACT
水生昆虫是大型无脊椎动物,生活在淡水水体底部或与之相关。在水生环境中,它们在养分循环中的关键作用再怎么强调也不为过。水生无脊椎动物是鱼类碎屑中不可获得的营养物质与蛋白质物质之间的纽带[1,2]。它们在快速追踪腐烂的有机物质分解成较小的无机形式(如硝酸盐和磷酸盐)方面的作用也得到了充分的证明。这些营养物质通常被生态系统中的水生植物所利用。水生昆虫常被用作生物指标,在环境污染和水质监测中发挥着重要作用[4,5]。水生昆虫对环境扰动的敏感性和耐受力在不同目、不同种之间差别很大。水生昆虫在河道中任意一点的组成和分布是该水域水质状况的反映。这使得水生昆虫成为污染和水质评价的重要工具。水体的物化参数[温度、溶解氧(DO)、生化需氧量(BOD)和浊度]会影响水生昆虫的物种丰富度。昆虫群落的结构组成一直是水生系统研究的热点。一个物种的个体的存在或不存在提供了很少的信息
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引用次数: 2
In vitro bioactivity and phytochemical analysis of two marine macro-algae 两种海洋巨藻的体外生物活性及植物化学分析
Pub Date : 2017-09-26 DOI: 10.12980/JCLM.5.2017J7-124
Ravi Cyril, Revathi Lakshmanan, A. Thiyagarajan
Marine macro-algae also called as seaweeds are rich in minerals, polyunsaturated fatty acids and vitamins; hence it could potentially be exploited for both human and animal health applications[1,2]. Around 600 secondary metabolites have been so far identified from seaweeds[3] and compounds derived from them are associated with broad range of biological activities[4,5]. The emergence of antibiotic resistant microorganisms in aquaculture is a key obstacle to their extensive use. The residue of these chemicals in aquaculture products has also been reported as a serious concern for consumers[6]. The prevention and treatment of these infectious agents by applying natural products from marine organisms appears to be a possible alternative source[7]. The desirable physiological and morphological characteristics of erythrocytes made them an effective tool in drug testing and delivery[8,9]. Oxidative damage to the erythrocyte membrane leads to hemolysis and compounds having antioxidant properties have long term action on hemolysis[10]. Phytochemicals such as flavonoids, polyphenols and phlobatanins inhibited proliferation of cancer cells and influenced anti-inflammatory responses[11]. Sargassum wightii (family: Phaeophyceae) (S. wightii) is widely distributed in the southern coasts of India, harbors good amount of flavonoids and is an ideal target for medical and industrial applications[12]. Sargassum sp. contains various secondary metabolites with biological activities[13] and has also been used in the treatment of chronic bronchitis, hypertension, edema, goiter and tuberculosis of lymph nodes[14]. Gracilaria verrucosa (family: Rhodophyceae) (G. verrucosa) prevalent in Indian coast is known for its food value. The phycocolloids have a high growth rate owing to temperature tolerance and are rich in primary and secondary metabolites[15]. Gracilaria species have small esterification cell wall and phycocolloids, the main source of agar α-(1,4)-3,6-anhydroL-galactose and β-(1,3)-D-galactose that are important for the industrial and biotechnological applications[16,17]. With this backdrop, the present study has been undertaken to compare the in vitro biological activity of solvent extracts of S. wightii and G. verrucosa. ARTICLE INFO ABSTRACT
海洋大型海藻也被称为海藻,富含矿物质、多不饱和脂肪酸和维生素;因此,它有可能被用于人类和动物健康应用[1,2]。迄今为止,已从海藻中鉴定出约600种次级代谢产物[3],其衍生的化合物与广泛的生物活性有关[4,5]。水产养殖中抗生素抗性微生物的出现是其广泛使用的主要障碍。据报道,这些化学物质在水产养殖产品中的残留也引起了消费者的严重关注[6]。通过应用海洋生物的天然产物预防和治疗这些传染源似乎是一种可能的替代来源[7]。红细胞理想的生理和形态特征使其成为药物测试和递送的有效工具[8,9]。红细胞膜的氧化损伤会导致溶血,具有抗氧化特性的化合物对溶血有长期作用[10]。类黄酮、多酚和根皮素等植物化学物质抑制癌症细胞的增殖,并影响抗炎反应[11]。马尾藻(马尾藻科:Phaeophyceae)(S.wightii)广泛分布于印度南部海岸,含有大量黄酮类化合物,是医学和工业应用的理想靶标[12]。马尾藻含有各种具有生物活性的次级代谢产物[13],还被用于治疗慢性支气管炎、高血压、水肿、甲状腺肿和淋巴结结核[14]。疣状龙须菜(科:红藻科)(G.verrucosa)在印度海岸流行,以其食物价值而闻名。由于耐温性,类藻具有较高的生长速率,并且富含初级和次级代谢产物[15]。龙须菜具有较小的酯化细胞壁和类藻,是琼脂α-(1,4)-3,6-脱水L-半乳糖和β-(1,3)-D-半乳糖的主要来源,对工业和生物技术应用很重要[16,17]。在这种背景下,本研究对威格提和疣状毛霉菌溶剂提取物的体外生物活性进行了比较。文章信息摘要
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引用次数: 9
The alteration of biochemical parameters leading to organ damage during Plasmodium berghei ANKA infection in mice 伯氏疟原虫ANKA感染小鼠时生化参数的改变导致器官损伤
Pub Date : 2017-09-26 DOI: 10.12980/JCLM.5.2017J7-128
Sukanya Pattarapo, Kittikarn Kittikarn Ratanavijarn, Voravuth Somsak
*Corresponding author: Voravuth Somsak, Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. Tels: +66 7567 2780, +66 8 9800 9939 E-mail: voravuth.so@wu.ac.th All the experiments involving mice were conducted in accordance to the International Animal Ethics Committee NIH Guidelines and approved by the Animal Ethical Committee, Western University, Thailand (WTU-AE-4328). Foundation Project: Supported by Research and Academic Science Service Department, Western University (Grant No. WTU-2016-068) The journal implements double-blind peer review practiced by specially invited international editorial board members.
*通讯作者:Voravuth Somsak,瓦利拉克大学联合健康科学学院医学技术系,泰国,Nakhon Si Thammarat 80161。电话:+66 7567 2780,+66 8 9800 9939电子邮件:voravuth.so@wu.ac.th所有涉及小鼠的实验均根据国际动物伦理委员会NIH指南进行,并经泰国西部大学动物伦理委员会批准(WTU-AE-4328)。基金资助项目:由西部大学研究与学术服务部资助(批准号:WTU-2016-068),期刊实行国际编委会特邀成员双盲同行评审。
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引用次数: 1
Chemical composition, antibacterial and antifungal activities of Saudi Arabian Mentha longifolia L. essential oil 沙特阿拉伯长叶薄荷精油的化学成分及抑菌抑菌活性
Pub Date : 2017-09-26 DOI: 10.12980/JCLM.5.2017J7-108
Nagarjuna Reddy Desam, A. J. Al-Rajab, Mukul Sharma, Mary Moses Mylabathula, R. R. Gowkanapalli, Al-Zahrani Mohammed
Objective: To investigate the chemical composition, in vitro antibacterial and antifungal activity of Mentha longifolia L. essential oils using aqueous extract. Methods: Mentha longifolia L. essential oils were extracted using hydrodistillation with Clevenger apparatus for 3 h and the yield of the essential oil was calculated. Essential oils were qualitatively and quantitatively analyzed with gas chromatography and gas chromatographymass spectrometer. For these essential oils, antibacterial and antifungal activity against human pathogens were evaluated. Results: Nineteen chemical constituents representing 99.72% of the essential oil were found, comprising menthone (39.55%), isopulegone (30.49%), eucalyptol (10.38%), and α-terpineol (3.15%); these were major components, and others were minor components. The essential oil showed strong antibacterial activity against Staphylococcus aureus [(35.24 � 0.13) mm], Enterococcus faecalis [(32.12 � 0.12) mm] and Bacillus cereus [(30.06 � 0.04) mm], as well as antifungal activity against Aspergillus flavus [(38.02 � 0.06) mm], Alternaria alternaria [(35.26 � 0.12) mm], and Penicillum spp [(34.14 � 0.02) mm]. Conclusions: It seems that the essential oils derived from the Mentha longifolia L. species could be used as a natural source of antimicrobial agents.
目的:研究长叶薄荷挥发油的化学成分、体外抗菌及抗真菌活性。方法:采用Clevenger装置,采用加氢蒸馏法提取长叶薄荷精油,提取时间3h,并计算精油的收率。采用气相色谱和气相色谱质谱仪对精油进行了定性和定量分析。对这些精油的抗菌和抗真菌活性进行了评估。结果:挥发油中含有19种化学成分,占总精油的99.72%,分别为薄荷脑(39.55%)、异ulegone(30.49%)、桉树醇(10.38%)和α-萜品醇(3.15%);这些是主要的组成部分,其他的是次要的组成部分。该精油对金黄色葡萄球菌具有很强的抗菌活性[(35.24� 0.13)mm],粪肠球菌[(32.12� 0.12)mm]和蜡样芽孢杆菌[(30.06� 0.04)mm],以及对黄曲霉的抗真菌活性[(38.02� 0.06)mm],交链格孢[(35.26� 0.12)mm]和青霉属[(34.14� 0.02)毫米]。结论:从长叶薄荷中提取的精油可以作为一种天然的抗菌剂来源。
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引用次数: 9
Curcumin – A review on multipotential phytocompound 姜黄素——多能植物化合物综述
Pub Date : 2017-09-26 DOI: 10.12980/jclm.5.2017J7-115
N. Gilani, H. Basharat, H. Qureshi
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引用次数: 1
Correlation study of some physico-chemical parameters and benthic macroinvertebrates metrics on the ecological impacts of floriculture industries along Wedecha River, Debrezeit, Ethiopia 埃塞俄比亚Debrezeit Wedecha河沿河花卉产业生态影响的理化参数与底栖大型无脊椎动物指标的相关性研究
Pub Date : 2017-09-26 DOI: 10.12980/JCLM.5.2017J7-31
S. M. Tamiru, Seyoum Leta Asfaw, Seyoum Mengistu Yilma
Sisay Misganaw Tamiru, Seyoum Leta Asfaw, Seyoum Mengistu Yilma Department of Development and Environmental Management Studies, University of Gondar, PoBox 196, Gondar, Ethiopia College of Natural Science, Environmental Science Program, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia College of Natural Science, Department of Zoological Sciences, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia Journal of Coastal Life Medicine 2017; 5(10): 433-440
Sisay Misganaw Tamiru、Seyoum Leta Asfaw、Seyoum Mengistu Yilma贡达尔大学发展与环境管理研究系,邮政信箱196,贡达尔,埃塞俄比亚自然科学学院,环境科学项目,亚的斯亚贝巴大学,邮政信箱1176,亚的斯亚贝巴,埃塞俄比亚自然学院,亚的斯亚贝巴大学动物科学系。方框1176,亚的斯亚贝巴,埃塞俄比亚海岸生命医学杂志2017;5(10):433-440
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引用次数: 9
Survey of parasites of two fish species (Tilapia zillii and Clarias gariepinus) in Ase River Catchment, Delta State, Nigeria. 尼日利亚三角洲州Ase河流域zillii罗非鱼和Clarias gariepinus两种鱼类寄生虫调查。
Pub Date : 2017-09-26 DOI: 10.12980/JCLM.5.2017J7-126
E. E. Ito
Tropical freshwater fishes such as Tilapia zillii (T. zillii) and Clarias gariepinus (C. gariepinus) serve as definitive/transport or intermediate host in the developmental cycle of many species of protozoan, metazoan and crustacean parasites[1]. Parasites are invertebrate organisms; some are free-living and can become opportunistic parasites while the obligate parasites require hosts for their survival and reproduction. Both opportunistic and obligate parasites are found in fish hosts but most parasitic diseases in fish are generally caused by obligate parasites[2]. In fisheries, some parasites may be highly pathogenic and contribute to high fish mortalities and economic losses or threaten the abundance and diversity of indigenous fish species[3,4]. T. zillii and C. gariepinus are the most common sources of protein for humans and other animals in the tropics[5-7]. Fish interacts with the various levels of food chain and influences the structures of rivers, lakes, streams and estuaries, since they are usually restricted to particular modes of life related to their food sources and reproductive requirements[8,9]. Like other animals, fish is also afflicted by endoand ectoparasites, especially protozoans and helminths causing heavy mortality[10]. Fish parasites and diseases constitute one of the most important problems confronting fish farmers today[11]. Pathological conditions resulting from parasites and diseases cause high magnitude of epidemics under crowded and other unnatural conditions[12]. The role of freshwater fish in transmitting parasites ARTICLE INFO ABSTRACT
热带淡水鱼类,如罗非鱼(T.zillii)和加里皮氏罗非鱼(C.gariepinus),在许多原生动物、后生动物和甲壳类动物寄生虫的发育周期中充当最终/运输或中间宿主[1]。寄生虫是无脊椎动物;一些是自由生活的,可以成为机会性寄生虫,而专性寄生虫需要宿主才能生存和繁殖。机会寄生虫和专性寄生虫都存在于鱼类宿主中,但鱼类中的大多数寄生虫病通常是由专性寄生虫引起的[2]。在渔业中,一些寄生虫可能具有高致病性,导致鱼类的高死亡率和经济损失,或威胁当地鱼类的丰富性和多样性[3,4]。齐利T.zillii和加里皮C.gariepinus是热带地区人类和其他动物最常见的蛋白质来源[5-7]。鱼类与食物链的各个层面相互作用,并影响河流、湖泊、溪流和河口的结构,因为它们通常被限制在与其食物来源和繁殖需求相关的特定生活模式[8,9]。和其他动物一样,鱼类也受到体内外寄生虫的折磨,尤其是原生动物和蠕虫,它们会导致严重的死亡[10]。鱼类寄生虫和疾病是当今养鱼户面临的最重要问题之一[11]。寄生虫和疾病引起的病理条件在拥挤和其他非自然条件下会导致高度流行病[12]。淡水鱼在传播寄生虫中的作用文章信息摘要
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引用次数: 2
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海岸生命医学杂志(英文版)
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