Pub Date : 2023-01-01DOI: 10.4236/ajmb.2023.131001
Rhoda Elizabeth King, J. Kimotho, R. Macharia, Faith Ndung'u, S. Nzou, R. M. Irekwa
{"title":"Identification and Characterization of Hepatitis B Virus Immune Escape Mutants in Kenya","authors":"Rhoda Elizabeth King, J. Kimotho, R. Macharia, Faith Ndung'u, S. Nzou, R. M. Irekwa","doi":"10.4236/ajmb.2023.131001","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131001","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4236/ajmb.2023.134014
Yannick Frédéric Okouakoua, N. Mokémiabeka, C. A. Kayath, Moïse Doria Kaya-Ongoto, S. A. Nzaou, Nkalla Lambi Ndelani, Etienne Nguimbi
{"title":"Molecular Profiling of Sugarcane Wine, a Traditional Fermented Beverage (Loungouila) from Madingou","authors":"Yannick Frédéric Okouakoua, N. Mokémiabeka, C. A. Kayath, Moïse Doria Kaya-Ongoto, S. A. Nzaou, Nkalla Lambi Ndelani, Etienne Nguimbi","doi":"10.4236/ajmb.2023.134014","DOIUrl":"https://doi.org/10.4236/ajmb.2023.134014","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4236/ajmb.2023.133013
R. Mahmoud, A. A. Dahab, Gehan Ahmed Mahmoud, M. El-Wahab, E. El-Bassel, E. Mahdy
{"title":"Ampelographic and Genetic Diversity Assessment of Some Local Grape Genotypes under Egyptian Conditions","authors":"R. Mahmoud, A. A. Dahab, Gehan Ahmed Mahmoud, M. El-Wahab, E. El-Bassel, E. Mahdy","doi":"10.4236/ajmb.2023.133013","DOIUrl":"https://doi.org/10.4236/ajmb.2023.133013","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4236/ajmb.2023.132009
Dawala Koromtili Oumar, Matthew Mutinda Munyao, A. Mwangi, R. Waihenya, Peter Kipkemboi Rotich, R. M. Irekwa, Tonny Teya Nyandwaro, C. W. Njoroge, J. Yego, Primrose Muthoni Ndungu, Sharlene Kerubo Mageto, Damaris Mutethya Kilei, Otilmoi Poul Stephen, Nicole Sian Tanchu, Grace Ng’endo Kanyita, Shingo Inoue, Lucy Gitau, S. Nzou
{"title":"Use of Polyclonal Antibody for the Diagnosis of Human African Trypanosomiasis","authors":"Dawala Koromtili Oumar, Matthew Mutinda Munyao, A. Mwangi, R. Waihenya, Peter Kipkemboi Rotich, R. M. Irekwa, Tonny Teya Nyandwaro, C. W. Njoroge, J. Yego, Primrose Muthoni Ndungu, Sharlene Kerubo Mageto, Damaris Mutethya Kilei, Otilmoi Poul Stephen, Nicole Sian Tanchu, Grace Ng’endo Kanyita, Shingo Inoue, Lucy Gitau, S. Nzou","doi":"10.4236/ajmb.2023.132009","DOIUrl":"https://doi.org/10.4236/ajmb.2023.132009","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70514064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/ajmb.2022.124015
Minas Mohamed Balla, A. Mergani, Mohamed Elamin A. M. E. Medani, A. Abakar, A. Dafalla
Background: Streptococcus pyogenes (group A streptococcus, GAS) is an important human bacterial pathogen. This organism possesses many virulence factors, Streptococcal pyrogenic exotoxinone of these. Aim: Detection of Streptococcal pyrogenic exotoxin SpeA and SpeB in isolated Streptococcus pyogenes. Methods: Tow hundred throat swab samples were collected from children with pharyngitis referred to Pediatric Teaching hospital and ENT hospital Wad medani, Sudan, from January to November 2021. The ques-tionnaire was filled out to collect clinical and demographic data. Throat swabs were collected and processed with the standard microbiological pro-cedure to isolate Streptococcus pyogenes. Antimicrobial susceptibility testing was done on all GAS isolates using the Kirby Bauer disk diffusion method according to clinical laboratory standard institute (CLSI) guidelines. Detection of Spy 1258 gene and Streptococcal pyrogenic exotoxins SpeA and SpeB were done by using Multiplex PCR. Results: Amongst the Tow hundred collected samples fifty-one isolates (25.5%) were identified as S. pyogenes. Antibiotic susceptibility testing showed that all the GAS isolates were sensitive to Azithromycin and Penicillin. Sensitivity to Erythromycin, Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%, respectively. SpeA was detected in 17 (33.3%) and SpeB in 48 (94.1%). Conclusion: Streptococcal pyrogenic exotoxin genes SpeA and SpeB were detected in 17 (33.3%) and 48 (94.1%) respectively of Streptococcus pyogenes isolates.
{"title":"Streptococcal Pyrogenic Exotoxin Genes SpeA and SpeB in Isolates of <i>Streptococcus pyogenes</i> from Children with Pharyngitis, Gezira State, Sudan","authors":"Minas Mohamed Balla, A. Mergani, Mohamed Elamin A. M. E. Medani, A. Abakar, A. Dafalla","doi":"10.4236/ajmb.2022.124015","DOIUrl":"https://doi.org/10.4236/ajmb.2022.124015","url":null,"abstract":"Background: Streptococcus pyogenes (group A streptococcus, GAS) is an important human bacterial pathogen. This organism possesses many virulence factors, Streptococcal pyrogenic exotoxinone of these. Aim: Detection of Streptococcal pyrogenic exotoxin SpeA and SpeB in isolated Streptococcus pyogenes. Methods: Tow hundred throat swab samples were collected from children with pharyngitis referred to Pediatric Teaching hospital and ENT hospital Wad medani, Sudan, from January to November 2021. The ques-tionnaire was filled out to collect clinical and demographic data. Throat swabs were collected and processed with the standard microbiological pro-cedure to isolate Streptococcus pyogenes. Antimicrobial susceptibility testing was done on all GAS isolates using the Kirby Bauer disk diffusion method according to clinical laboratory standard institute (CLSI) guidelines. Detection of Spy 1258 gene and Streptococcal pyrogenic exotoxins SpeA and SpeB were done by using Multiplex PCR. Results: Amongst the Tow hundred collected samples fifty-one isolates (25.5%) were identified as S. pyogenes. Antibiotic susceptibility testing showed that all the GAS isolates were sensitive to Azithromycin and Penicillin. Sensitivity to Erythromycin, Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%, respectively. SpeA was detected in 17 (33.3%) and SpeB in 48 (94.1%). Conclusion: Streptococcal pyrogenic exotoxin genes SpeA and SpeB were detected in 17 (33.3%) and 48 (94.1%) respectively of Streptococcus pyogenes isolates.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/ajmb.2022.124016
A. Sharmin, F. Aminuzzaman, T. H. Ansari, M. Ahmed
{"title":"Morphological, Cultural, Molecular and Pathogenic Variation of <i>Bipolaris sorokiniana</i> in Wheat","authors":"A. Sharmin, F. Aminuzzaman, T. H. Ansari, M. Ahmed","doi":"10.4236/ajmb.2022.124016","DOIUrl":"https://doi.org/10.4236/ajmb.2022.124016","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/ajmb.2022.121001
Huan Zhao, Xiaoyu Jiang, Ling-ling Lu, Qing Lin
Acute respiratory infection in children (ARTI) is the most common childhood infectious disease, and its pathogens include bacteria, fungi, viruses, chlamydia, mycoplasma and rickettsia. In recent years, with the continuous development of pathogen detection methods, the diagnosis and treatment of acute respiratory infections has received more and more attention from clini-cians. The clinical diagnosis and treatment of acute respiratory infections in children and the research of laboratory detection methods have also been continuously developed. The manuscript presents a review of progress in the clinical diagnosis, treatment and laboratory testing of acute respiratory infections in children by collecting references.
儿童急性呼吸道感染(Acute respiratory infection in children, ARTI)是最常见的儿童传染病，其病原体包括细菌、真菌、病毒、衣原体、支原体和立克次体。近年来，随着病原体检测方法的不断发展，急性呼吸道感染的诊断和治疗越来越受到临床医生的重视。儿童急性呼吸道感染的临床诊治和实验室检测方法的研究也在不断发展。该手稿通过收集参考文献，介绍了在儿童急性呼吸道感染的临床诊断、治疗和实验室检测方面的进展。
{"title":"New Progress in Clinical Diagnosis, Treatment and Laboratory Testing of Acute Respiratory Infections in Children","authors":"Huan Zhao, Xiaoyu Jiang, Ling-ling Lu, Qing Lin","doi":"10.4236/ajmb.2022.121001","DOIUrl":"https://doi.org/10.4236/ajmb.2022.121001","url":null,"abstract":"Acute respiratory infection in children (ARTI) is the most common childhood infectious disease, and its pathogens include bacteria, fungi, viruses, chlamydia, mycoplasma and rickettsia. In recent years, with the continuous development of pathogen detection methods, the diagnosis and treatment of acute respiratory infections has received more and more attention from clini-cians. The clinical diagnosis and treatment of acute respiratory infections in children and the research of laboratory detection methods have also been continuously developed. The manuscript presents a review of progress in the clinical diagnosis, treatment and laboratory testing of acute respiratory infections in children by collecting references.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/ajmb.2022.122003
Keita Odanaka
Candida albicans proliferates in the skin and oral cavity and is the causative agent of candida dermatitis and oral candidiasis. C. albicans is known to form biofilms on oral mucosa and denture surfaces. Formation of biofilms deteri-orates the permeability of antifungal drugs, decreasing their effectiveness. Therefore, in this study, I identified a compound with inhibitory activity against C. albicans biofilm formation. Heat shock protein 90 was selected as the target protein, and a potential ligand for the same was extracted and identified as 2-(4-methylpiperazin-1-yl)cyclopentanol. C. albicans was then cultured with varying concentrations of this compound: 0 mmol/L, 0.63 mmol/l. 2.5 mmol/l, and 10 mmol/l, and biofilm formation was measured via crystal violet assay. The findings demonstrated that 2-(4-methylpiperazin-1-yl)cyclo-pentanol substantially inhibits biofilm formation when added at a concentration of 0.63 mmol/l or higher. It is suggested that C. albicans could be elimi-nated more efficiently using this compound in combination with the existing antifungal drug miconazole. Further, the compound may also be useful as a disinfectant for medical devices, such as catheters, to prevent the formation of C. albicans biofilms.
{"title":"Identification of a <i>Candida albicans</i> Biofilm Inhibitor","authors":"Keita Odanaka","doi":"10.4236/ajmb.2022.122003","DOIUrl":"https://doi.org/10.4236/ajmb.2022.122003","url":null,"abstract":"Candida albicans proliferates in the skin and oral cavity and is the causative agent of candida dermatitis and oral candidiasis. C. albicans is known to form biofilms on oral mucosa and denture surfaces. Formation of biofilms deteri-orates the permeability of antifungal drugs, decreasing their effectiveness. Therefore, in this study, I identified a compound with inhibitory activity against C. albicans biofilm formation. Heat shock protein 90 was selected as the target protein, and a potential ligand for the same was extracted and identified as 2-(4-methylpiperazin-1-yl)cyclopentanol. C. albicans was then cultured with varying concentrations of this compound: 0 mmol/L, 0.63 mmol/l. 2.5 mmol/l, and 10 mmol/l, and biofilm formation was measured via crystal violet assay. The findings demonstrated that 2-(4-methylpiperazin-1-yl)cyclo-pentanol substantially inhibits biofilm formation when added at a concentration of 0.63 mmol/l or higher. It is suggested that C. albicans could be elimi-nated more efficiently using this compound in combination with the existing antifungal drug miconazole. Further, the compound may also be useful as a disinfectant for medical devices, such as catheters, to prevent the formation of C. albicans biofilms.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/ajmb.2022.123008
Salma Ibrahim, E. A. Mohammed, S. Taha, S. Yousif, H. Omer, O. Omer, Mirghani Seif-Elnasr, Seitelbanat Yassin, Y. Mohammed, O. A. Elhasan, Mohamed Taj-Eldin, A. Abakar, E. A. Ahmed, S. Elzaki
{"title":"Incidence of Oxa23 and Oxa51 Genes Associated with Bacterial Isolated from Patients with Urosepsis: Single Centre Prespective","authors":"Salma Ibrahim, E. A. Mohammed, S. Taha, S. Yousif, H. Omer, O. Omer, Mirghani Seif-Elnasr, Seitelbanat Yassin, Y. Mohammed, O. A. Elhasan, Mohamed Taj-Eldin, A. Abakar, E. A. Ahmed, S. Elzaki","doi":"10.4236/ajmb.2022.123008","DOIUrl":"https://doi.org/10.4236/ajmb.2022.123008","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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