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Lu pour vous 为你读
Pub Date : 2023-01-01 DOI: 10.1007/BF02970317
C. Treille
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引用次数: 0
Analyse de livres 书籍分析
Pub Date : 2022-10-01 DOI: 10.1007/BF02973618
J. -. Dubois de Montreynaud, H. Lindner, L. Chevrot, D. Olive, G. Chanteclair
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引用次数: 0
Annonces de Congrès 大会公告
Pub Date : 2022-10-01 DOI: 10.1007/BF02965778
Annonces de Congrbs
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引用次数: 0
Analyses de Livres 书籍分析
Pub Date : 2022-09-28 DOI: 10.1007/BF03016661
P. Dieterling
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引用次数: 0
Annonces de congrès 大会公告
Pub Date : 2022-08-01 DOI: 10.1007/BF03023691
M. Giovannini, J. Godchaux, T. Helbert
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引用次数: 0
Annonces de Congrès 大会公告
Pub Date : 2022-06-10 DOI: 10.1007/BF03006063
Annonces de Congrbs
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引用次数: 0
Lu pour vous 为你而读
Pub Date : 2022-06-01 DOI: 10.1007/BF02973733
P. Cotton
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引用次数: 0
Lu pour vous 为你而读
Pub Date : 2022-05-01 DOI: 10.1007/BF02965398
Lorraine Albert, Lu pour vous
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引用次数: 0
Microfluidics-based strategies for molecular diagnostics of infectious diseases. 基于微流控技术的传染病分子诊断策略。
IF 16.7 Pub Date : 2022-03-18 DOI: 10.1186/s40779-022-00374-3
Xin Wang, Xian-Zhe Hong, Yi-Wei Li, Ying Li, Jie Wang, Peng Chen, Bi-Feng Liu

Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

传统的传染病检测诊断策略需要台式仪器,不适合用于床旁检测(POCT)。新兴的微流控技术是一种高度微型化、自动化和集成化的技术,有可能取代传统方法,进行快速、低成本、准确的现场诊断。分子诊断作为最有效的病原体检测方法被广泛应用于微流控设备中。本综述从学术角度和工业前景两方面总结了基于微流控技术的传染病分子诊断的最新进展。首先,我们介绍了典型的片上核酸处理过程,包括样品预处理、扩增和信号读出。然后,比较了四类微流控平台的特点、优缺点。我们还进一步讨论了数字检测在核酸绝对定量中的应用。我们总结了基于微流控技术的经典和最新商业分子诊断设备,以证明目前的市场状况。最后,我们提出了基于微流控技术的传染病诊断的未来发展方向。
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引用次数: 0
Analyse de livres 书籍分析
Pub Date : 2022-03-01 DOI: 10.1007/BF02973656
A. Jacquier
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引用次数: 0
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