Acta virologica最新文献

英文中文

Acetylation of late expression factor 4 is crucial for the transcription and proliferation of Bombyx mori nucleopolyhedrovirus. 晚期表达因子4的乙酰化对家蚕核多角体病毒的转录和增殖至关重要。

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_109

Jonas Ngowo, Xu Gao, Yajie Zhu, Fuxiang Mao, Jihai Lei, E. Obeng, Wei Yu

Late expression factor 4 (LEF4), RNA polymerase subunit of Bombyx mori nucleopolyhedrovirus (BmNPV), plays an enzymatic role to enhance the capping of pre-mRNA of late and very late genes. Lysine acetylation is a post-translational modification process having many important functions associated with the regulation of a gene expression. Our previous study on lysine acetylome in BmNPV infected BmN cells showed that LEF 4 was acetylated at lysine 76 (K76). However, it is still unclear whether the modification of K76 residue contributes to the modulation of viral gene transcription. To elucidate the role played by acetylation or deacetylation of LEF4 K76 in the transcription of viral genes, we constructed acetylation mimicking and deacetylation mimicking mutant virus, K76Q and K76R, respectively. We then transfected BmN cells with these mutants and analyzed the level of pre-mRNA at different times. The K76R showed a significant decrease in the mRNA transcription level of vp39 and p10 genes at 48 and 72 h post-transfection, while K76Q did not show any significant changes compared with lef4-Wt. We further detected the virus titer of lef4-Wt, K76Q [et] K76R, and it was found that K76R impaired the virus infectivity ability at 72 and 96 h, while K76Q did not affect the virus infectivity. Moreover, the yeast two hybrid technique (Y2H) showed that both mutants (K76Q [et] K76R) affected the association of LEF 4 with the P47 protein. Taken together, these results indicated that acetylation modification of K76 is important for the proper transcription of late and very late genes, and the effectiveness of viral infection. Keywords: BmNPV lef4 gene; lysine acetylation, late genes transcription; BmNPV p47 gene; infectivity.

家蚕核多角体病毒(BmNPV)的RNA聚合酶亚基——晚期表达因子4 (Late expression factor 4, LEF4)在增强晚期和极晚期基因的前mrna的capping中起酶促作用。赖氨酸乙酰化是一个翻译后修饰过程，具有许多与基因表达调控相关的重要功能。我们之前对BmNPV感染的BmN细胞赖氨酸乙酰化的研究表明，lef4在赖氨酸76 (K76)处乙酰化。然而，目前尚不清楚K76残基的修饰是否参与了病毒基因转录的调节。为了阐明LEF4 K76乙酰化或去乙酰化在病毒基因转录中的作用，我们分别构建了乙酰化模拟和去乙酰化模拟突变病毒K76Q和K76R。然后我们用这些突变体转染BmN细胞，并分析不同时间pre-mRNA的水平。K76R在转染后48和72 h vp39和p10基因的mRNA转录水平显著降低，而K76Q与lef4-Wt相比没有明显变化。我们进一步检测了lef4-Wt、K76Q [et] K76R的病毒滴度，发现K76R在72和96 h时削弱了病毒的感染性，而K76Q不影响病毒的感染性。此外，酵母双杂交技术(Y2H)表明，两个突变体(K76Q [et] K76R)都影响了lef4与P47蛋白的关联。综上所述，这些结果表明，K76的乙酰化修饰对于晚期和极晚期基因的正确转录以及病毒感染的有效性至关重要。关键词:BmNPV lef4基因;赖氨酸乙酰化，晚期基因转录;BmNPV p47基因;传染性。

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引用次数: 0

Suitability of different plant species for experimental agroinfection with Plum pox virus-based expression vector for potential production of edible vaccines. 以梅痘病毒为基础的表达载体对不同植物侵染试验的适宜性及可食用疫苗的潜在生产。

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_111

Adam Achs, M. Glasa, P. Alaxin, Z. Šubr

Nine herbaceous plant species were tested for susceptibility to Plum pox virus (PPV) by Agrobacterium-mediated delivery of its infectious cDNA clone. Two of them became infected, namely spinach (local infection) and oilseed poppy (systemic infection). As a control, PPV infection was successfully established in plum seedlings following agroinfiltration, thus providing the first report of agroinfection in Prunus species. According to our results, oilseed poppy can be considered as a candidate host for the production of edible vaccines by a PPV-derived expression vector. Keywords: agroinfiltration; virus host; poppy; spinach.

采用农杆菌介导的方法，检测了9种草本植物对李痘病毒(PPV)的易感性。其中两种感染，即菠菜(局部感染)和油籽罂粟(全身感染)。作为对照，PPV侵染在灌浆后成功地在李树幼苗中建立，首次报道了李树属植物的土壤侵染。根据我们的研究结果，油籽罂粟可以被认为是ppv衍生表达载体生产可食用疫苗的候选宿主。关键词:agroinfiltration;病毒主机;罂粟花;菠菜。

引用次数: 1

Isolation and characterization of novel Spodoptera exigua nucleopolyhedrovirus strains in Turkey and their potential for use in biological control 土耳其新夜蛾核多角体病毒株的分离、鉴定及其生物防治潜力

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_407

D. Gencer, Z. Bayramoğlu, Çetin Mutlu, I. Demir

引用次数: 0

A gene expression signature that correlates with CD8+T cell expansion in acute Epstein-Barr virus infection. 急性eb病毒感染中与CD8+T细胞扩增相关的基因表达特征

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_303

Yijing Chen, Hanqing Wang, Xia Liu, Bing Luo

Acute infectious mononucleosis (AIM) is associated with Epstein-Barr virus (EBV) infection. We explored molecular mechanisms regarding the expression of CD8+T cells in convalescence stage (CONV). Differentially expressed genes (DEGs) were identified by analyzing GEO expression profiles. Subsequently, Gene Set Enrichment Analysis (GSEA), Protein-Protein Interactions (PPI) network, and gene-micro RNAs networks were used to identify hub genes and associated pathways. GSEA provided evidence that the top 3 gene sets in GSEA were all related to integrins. We identified ten hub genes in the PPI network and DGIdb was applied to predict potential targets that might reverse the expression of hub genes. Our study enhances a mechanistic understanding of the CD8+T cells expansion in acute EBV infection and provides potential treatment targets for further research. Keywords: acute infectious mononucleosis; bioinformatics; CD8+T cells; differentially expressed genes; EBV.

急性传染性单核细胞增多症(AIM)与eb病毒(EBV)感染有关。我们探讨了CD8+T细胞在恢复期(CONV)表达的分子机制。通过分析GEO表达谱，鉴定出差异表达基因(DEGs)。随后，利用基因集富集分析(GSEA)、蛋白-蛋白相互作用(PPI)网络和基因-微rna网络鉴定中心基因及其相关途径。GSEA提供的证据表明，GSEA中排名前3位的基因集均与整合素相关。我们在PPI网络中鉴定了10个枢纽基因，并应用DGIdb来预测可能逆转枢纽基因表达的潜在靶点。我们的研究增强了对急性EBV感染中CD8+T细胞扩增的机制理解，并为进一步研究提供了潜在的治疗靶点。关键词:急性传染性单核细胞增多症;生物信息学;CD8 + T细胞;差异表达基因;EBV。

引用次数: 0

Genomic phylogenetic analyses of four major hand, foot and mouth disease-related enteroviruses. 四种主要手足口病相关肠道病毒的基因组系统发育分析。

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_104

Yajuan Dong, Zhenzhou Wan, Shenwei Li, Jian-Hua Wang, Xia Jin, Guoying Yu, Chiyu Zhang

Enteroviruses had diverged into many types, some of which cause hand, foot and mouth disease (HFMD) in children. The predominant enterovirus types associated with HFMD are EVA71, CVA16, CVA6 and CVA10. Four enterovirus types were classified into subtypes based on VP1 sequences. However, the phylogenetics of these enteroviruses is rarely concerned at the genomic level. In this study, we performed the phylogenetic analyses of the EVA71, CVA16, CVA6 and CVA10 using available full-length genomic sequences. We found that the topologies of phylogenetic trees of full-length genomic sequences and VP1 sequences were almost consistent, except few subtypes of EVA71 and CVA10. The mean genetic divergence was 15.8-27% between subtypes and less than 12% within subtypes/sub-subtypes at genomic level. Comparison of phylogenetic topologies between genomic and VP1 sequences helped us to identify two new EVA71 inter-subtype recombinants RF01_CC4 and RF02_CC4. Furthermore, EVA71 subtypes C1 and C2 and CVA10 subtype D were found to originate through inter-subtype recombination. The genomic reference sequences of these enteroviruses are provided here for subtyping. The results provide important insights into the understanding of the evolution and epidemiology of the four enteroviruses. Keywords: enterovirus; hand; foot and mouth disease; classification; genetic distance; recombination.

肠病毒已经分化成许多类型，其中一些会导致儿童手足口病。与手足口病相关的主要肠道病毒类型为EVA71、CVA16、CVA6和CVA10。根据VP1序列将4种肠道病毒分为不同的亚型。然而，这些肠道病毒的系统发育很少在基因组水平上得到关注。在这项研究中，我们利用现有的全基因组序列对EVA71、CVA16、CVA6和CVA10进行了系统发育分析。研究发现，除了EVA71和CVA10的少数亚型外，VP1全长基因组序列与VP1序列的系统发育树拓扑结构基本一致。亚型间遗传差异均值为15.8 ~ 27%，亚型/亚亚型间遗传差异均值小于12%。通过比较基因组序列和VP1序列的系统发育拓扑结构，我们鉴定出两个新的EVA71亚型间重组基因RF01_CC4和RF02_CC4。此外，发现EVA71亚型C1和C2以及CVA10亚型D是通过亚型间重组产生的。这里提供了这些肠道病毒的基因组参考序列用于分型。这些结果为了解这四种肠道病毒的进化和流行病学提供了重要的见解。关键词:肠病毒;手;口蹄疫;分类;遗传距离;重组。

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引用次数: 0

Insights into the factors affecting synonymous codon usage bias in the coat protein of soil-borne sugar beet-infecting viruses 土传甜菜侵染病毒外壳蛋白同义密码子使用偏向性影响因素的研究

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_402

S. Farzadfar, R. Pourrahim

引用次数: 0

Interaction between proteins of the PPARγ and NFκB immune response pathways and rotavirus non-structural proteins. PPARγ和NFκB免疫反应通路蛋白与轮状病毒非结构蛋白的相互作用。

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_105

Dory Gómez, C. Guerrero

Cells infected with MA104 rotavirus and/or transfected with plasmids expressing NSP proteins, were analyzed for expression of cellular proteins related to NFκB and PPARγ pathways and evaluated through the ELISA, luminescence, flow cytometry and Western blot techniques. The association between cellular and viral (NSPs) proteins was examined by ELISA, epifluorescence and confocal microscopy techniques. It was observed that NSP1 protein interacts with RXR, NSP1, and NSP3 with PPARγ, NSP2 with p-IKKα/β and NSP5 with NFκB proteins. We have found that phosphorylated PPARγ is localized in cytoplasm and transcriptional activity of PPRE is diminished. These results lead to the conclusion, that RRV activates the proinflammatory pathway, increasing the expression of NFκB and possibly by PPARγ phosphorylation, its translocation to the nucleus is impeded, thus inactivating the proinflammatory pathway. Keywords: rotavirus; PPARγ; NFκB; NSPs; RRV.

对感染MA104轮状病毒和/或转染表达NSP蛋白的质粒的细胞进行NFκB和PPARγ通路相关蛋白的表达分析，并通过ELISA、发光、流式细胞术和Western blot技术进行评估。通过ELISA、荧光和共聚焦显微镜技术检测细胞和病毒(NSPs)蛋白之间的关联。NSP1蛋白与RXR、NSP1、NSP3蛋白与PPARγ、NSP2蛋白与p-IKKα/β、NSP5蛋白与NFκB蛋白相互作用。我们发现磷酸化的PPRE γ定位于细胞质中，PPRE的转录活性降低。这些结果可以得出结论，RRV激活促炎途径，增加NFκB的表达，并可能通过PPARγ磷酸化，阻碍其向细胞核的易位，从而使促炎途径失活。关键词:轮状病毒;PPARγ;NFκB;NSPs;RRV。

引用次数: 0

Genomic profiling of three progenies isolated from a swarm of eGFP-tagged canine distemper viruses undergoing 40 serial passages 从一群egfp标记的犬瘟热病毒中分离出的三个子代进行40次连续传代的基因组分析

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_408

Shuning Zhou, Ling Wang, Jiahui Lin, Ning Wang, Yongle Yu, Youming Zhang, Fuxiao Liu

引用次数: 0

Simultaneous detection of velogenic Newcastle disease virus of genotype XIII 2.2 from spot-billed pelican and backyard chicken: implications to the viral maintenance and spread. 在斑嘴鹈鹕和家鸡中同时检测到基因型为XIII 2.2的新城疫病毒:对病毒维持和传播的意义。

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_305

Deepthi Balam, Ratnamma Doddamane, Ramani Pushpa Rayudu, Shrikrishna Isloor, Veeregowda Belamaranahally, Himaja Maddireddy, Muralidhar Metta

Despite the widespread occurence of Newcastle disease virus (NDV) in different avian species, there has been scanty reports on genetic characterization of NDV strains from wild birds in India. During 2017-18, a total of forty eight cloacal swab samples were collected from apparently healthy migratory birds (painted storks, n = 32 and spot-billed pelicans, n = 16) at the Telineelapuram bird sanctuary of Andhra Pradesh, India. NDV was isolated from a spot-billed pelican (NDV/Pelican/Telineelapuram/2018) which is genetically identical to that isolated from a naturally infected backyard chicken flock (NDV/Chicken/SKLM-1/2018). The isolates are found to be velogenic based on mean death time, intracerebral pathogenicity index and the putative fusion protein cleavage site (112R-R-R-K-R-F117). Phylogenetic analysis based on full-length fusion gene classified the isolates into genotype XIII, sub-genotype 2.2, however these isolates demonstrated multiple amino acid substitutions in the critical domains of F and HN proteins. The pelican strain (MIG-9) was tested for its pathogenic and transmission potential in three-weeks-old broiler chickens and the isolate proved to be highly virulent to chickens. To the best of our knowledge, this is the first evidence for the role of spot-billed pelicans in the maintenance of virulent NDV and its transmission to chickens in India. This study further highlights the role of wild birds in NDV transmission and the need for enhanced biosecurity in commercial poultry operations. Keywords: Newcastle disease virus; Pelecanus philippensis; chicken; transmission; pathogenicity; India.

尽管新城疫病毒(NDV)在不同的鸟类中广泛存在，但在印度，关于新城疫病毒毒株遗传特征的报道很少。2017- 2018年，在印度安得拉邦的Telineelapuram鸟类保护区，从表面健康的候鸟(画鹳，n = 32)和斑嘴鹈鹕，n = 16)中共收集了48个阴道炎拭子样本。从斑嘴鹈鹕(NDV/ pelican /Telineelapuram/2018)中分离出NDV，该病毒与从自然感染的后院鸡群(NDV/ chicken /SKLM-1/2018)中分离出的NDV基因相同。根据平均死亡时间、脑内致病性指数和推测的融合蛋白裂解位点(112R-R-R-K-R-F117)，发现分离株具有速度性。基于全长融合基因的系统发育分析将分离株划分为基因型XIII，亚基因型2.2，但这些分离株在F和HN蛋白的关键结构域存在多个氨基酸替换。对鹈鹕毒株(MIG-9)在3周龄肉鸡中的致病性和传播潜力进行了检测，分离物对鸡具有高毒力。据我们所知，这是斑嘴鹈鹕在印度维持致命的NDV及其传播给鸡的作用的第一个证据。这项研究进一步强调了野鸟在新城疫病传播中的作用以及在商业家禽经营中加强生物安全的必要性。关键词:新城疫病毒;Pelecanus philippensis;鸡;传输;致病性;印度。

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引用次数: 1

Molecular identification and characterization of badnaviruses infecting sugarcane in Ethiopia. 侵染埃塞俄比亚甘蔗的恶性病毒的分子鉴定和鉴定。

IF 1.7 4区医学 Q3 Medicine

Acta virologica

Pub Date : 2022-01-01 DOI: 10.4149/av_2022_101

Simatsidk Haregu, D. Kidanemariam, A. Abraham

Sugarcane bacilliform virus (SCBV) is an economically important virus limiting sugarcane production worldwide. Although Ethiopia is a major sugarcane producer, and virus-like symptoms are frequently observed in sugarcane fields, there is a complete lack of information as to the occurrence, distribution and molecular properties of SCBV. This study was aimed to identify and characterize SCBV isolates in Ethiopia using molecular methods. Out of 292 leaf samples collected and tested by PCR, 76 samples (26% incidence level) were found SCBV-positive. Nucleotide sequence analysis results showed that three Ethiopian isolates (SCBV-EtS3, SCBV-EtS6 and SCBV-EtC10) shared high level of nucleotide identity (99.5-100%) among themselves and with SCBV isolates from China (accession numbers MH037614 and MH037915). Another isolate, SCBV-EtC2, shared maximum identity of 78% with the other three SCBV isolates from Ethiopia and 99.8% with SCBV isolates from China (KM214357 and KM214307). Based on phylogenetic analysis, isolates from Ethiopia were segregated into two different clusters. Isolates SCBV-EtS3, SCBV-EtS6 and SCBV-EtC10 clustered with SCBV-Q group and SCBV-EtC2 with SCBV-H group. This study provides information on the occurrence of SCBV for the first time in Ethiopia and also contributes to the understanding of the genetic diversity of SCBV. Keywords: Caulimoviridae; RNase H, Saccharum spp.; Sugarcane bacilliform virus.

甘蔗杆菌状病毒(SCBV)是世界范围内限制甘蔗生产的重要经济病毒。虽然埃塞俄比亚是一个主要的甘蔗生产国，而且在甘蔗田里经常观察到类似病毒的症状，但完全缺乏关于单叶结叶病毒的发生、分布和分子特性的信息。本研究旨在利用分子方法鉴定和表征埃塞俄比亚的SCBV分离株。PCR检测的292份叶片样本中，有76份(26%)呈scbv阳性。核苷酸序列分析结果显示，3株埃塞俄比亚分离株(SCBV- ets3、SCBV- ets6和SCBV- etc10)与中国分离株(鉴定号MH037614和MH037915)具有较高的核苷酸同源性(99.5-100%)。另一株分离株SCBV- etc2与来自埃塞俄比亚的其他3株SCBV分离株(KM214357和KM214307)的一致性最高，为78%，与来自中国的SCBV分离株(KM214357和KM214307)的一致性最高为99.8%。基于系统发育分析，埃塞俄比亚分离株被分为两个不同的聚类。分离株SCBV-EtS3、SCBV-EtS6和scbv - et10与SCBV-Q群聚集，SCBV-EtC2与SCBV-H群聚集。该研究首次提供了埃塞俄比亚SCBV发生的信息，也有助于了解SCBV的遗传多样性。关键词:Caulimoviridae;核糖核酸酶H，糖精;甘蔗杆菌状病毒。

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