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Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference最新文献

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Semester-long Projects 学期的项目
Katherine A. Sharp, Robin L. Cooper, Dawn Carter
This discussion is focused on some experiences of semester-long course projects. At Rochester Institute of Technology (RIT), the first year laboratory courses (150 students per semester) are semester-long projects. We have adapted the Small World Initiative project (antibiotics from soil bacteria) and have devised and adapted other projects as a lens to teach laboratory techniques and engage our beginning scientists in authentic inquiry. Examples include Daphnia ecotoxicology and Fall tree studies. This past year has been challenging with the COVID-19 pandemic and the need to adapt laboratory courses to include students who are learning online, as well as planning for a pivot to online for the entire class. At the University of Kentucky (UK), in a Jr/Sr level Animal Physiology Course with a lab (120 students per semester) and a neurophysiology lab (Sr level) with 16 students we have focused on a theme this year with the effect of bacterial induced sepsis and to focus in on the direct effect of endotoxin (Lipopolysaccharides, LPS) from gram-negative bacteria. This was approached as authentic scientific inquiry. The novel investigation appeared to stimulate student engagement and curiosity. In the neurophysiology class the project turned into a publication with all students as co-authors. In the animal physiology course, the topic was woven through the various bodily systems from the cell level to the whole body. What students found interesting is how little is known on the direct effects of LPS as compared to the downstream actions of cytokines. To bridge this across kingdoms, the effects of LPS on root growth in plants and sensitivity to subsequent exposure as a defense mechanism were compared. Firstpage
本讨论的重点是一学期课程设计的一些经验。在罗切斯特理工学院(RIT),第一年的实验课程(每学期150名学生)是一个学期的项目。我们改编了小世界倡议项目(从土壤细菌中提取抗生素),并设计和改编了其他项目,作为教授实验室技术和让我们的初级科学家参与真实调查的镜头。例子包括水蚤生态毒理学和秋天树的研究。过去的一年充满了挑战,因为COVID-19大流行,需要调整实验课程,使其包括在线学习的学生,并计划整个班级转向在线学习。在肯塔基大学(英国),在Jr/Sr水平的动物生理学课程(每学期120名学生)和神经生理学实验室(Sr水平)(16名学生)中,我们今年的主题是细菌诱导败血症的影响,并重点关注来自革兰氏阴性细菌的内毒素(脂多糖,LPS)的直接影响。这是一种真正的科学探究。这项新颖的调查似乎激发了学生的参与度和好奇心。在神经生理学课上,这个项目变成了一份所有学生共同撰写的出版物。在动物生理学课程中,这个主题贯穿了从细胞水平到整个身体的各种身体系统。学生们发现有趣的是,与细胞因子的下游作用相比,我们对LPS的直接作用知之甚少。为了跨越这一领域,我们比较了LPS对植物根系生长的影响以及对随后暴露的敏感性作为一种防御机制。珍宝
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引用次数: 0
Revisiting Mendel: use of a Behavioral Assay to Examine Inheritance of Traits in Drosophila 重新审视孟德尔:使用行为试验来检查果蝇性状的遗传
Jeffrey M. Chalfant, R. Cooper, Tawny Aguayo-Williams, Lexie, Holtzclaw, Madison Loveless, Jennifer Wilson, Doug Harrison
Using the established rules of Mendel and others, predicting the outcome of genetic crosses in model organisms is a common exercise for college students. Frequently, one uses visible phenotypic markers such as curly wings, eye color, and abnormal bristles to identify genetic outcomes. Yet many genetically-based traits, such as behavioral and physiological characteristics, are not easily observed. To demonstrate that such traits can likewise display classical genetic inheritance, we utilized an optogenetic system in Drosophila to modify response to light. We utilized the inheritance of behavioral responses associated with light-activated channelrhodopsin in motor neurons and body wall muscles. The frequency of responsive animals was quantified over multiple generations beginning with two pure-breeding (homozygous) strains, each containing one of the two components needed to produce the light-sensitive proteins. The use of light-sensitive channels to examine the predicted genetic outcomes is an approach which can be used in teaching classical genetic principles using non-traditional phenotypes. Green fluorescent protein (GFP) can be expressed to illustrate which cells are expressing channel rhodopsin. This introduces concepts of transgenesis, genetically-modified organisms, and genetic contributions to behavior. In addition to basic dominant and recessive allelic relationships, the experiments introduce more complex genetic concepts, such as epistasis, gene expression and cellular diversity, as well as physiological and behavioral traits of animals. This module is presented in a variety of ways depending on equipment availability and can be used in a hybrid or remote format with data provided. Firstpage
利用孟德尔和其他人的既定规则,预测模式生物基因杂交的结果是大学生的一项常见练习。通常,人们使用可见的表型标记,如卷曲的翅膀,眼睛颜色和异常的刚毛来识别遗传结果。然而,许多基于基因的特征,如行为和生理特征,是不容易观察到的。为了证明这些性状同样可以表现出经典的遗传,我们利用果蝇的光遗传系统来改变对光的反应。我们利用运动神经元和体壁肌肉中与光激活通道视紫红质相关的行为反应的遗传。对反应动物的频率进行了数代量化,从两个纯育种(纯合子)菌株开始,每个菌株含有产生光敏蛋白所需的两种成分之一。使用光敏通道来检查预测的遗传结果是一种可以用于使用非传统表型的经典遗传原理教学的方法。绿色荧光蛋白(GFP)可以表达,以说明哪些细胞表达通道视紫红质。本课程介绍了转基因、转基因生物和基因对行为的影响等概念。除了基本的显性和隐性等位基因关系外,实验还引入了更复杂的遗传概念,如上位性、基因表达和细胞多样性,以及动物的生理和行为特征。该模块根据设备可用性以多种方式呈现,可以混合或远程格式使用所提供的数据。珍宝
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引用次数: 0
Virtual Labs: Exploring New Boundaries in Teaching Biology 虚拟实验室:探索生物学教学的新边界
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引用次数: 0
Incorporating digitized natural history collections data into virtual ecology and evolutioncourse‐based research experiences 将数字化自然历史藏品数据整合到虚拟生态和进化过程研究体验中
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引用次数: 0
Student Perception of Upper Year Online Labs 学生对高年级在线实验室的看法
{"title":"Student Perception of Upper Year Online Labs","authors":"","doi":"10.37590/able.v42.abs57","DOIUrl":"https://doi.org/10.37590/able.v42.abs57","url":null,"abstract":"","PeriodicalId":72079,"journal":{"name":"Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78509826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a classroom size nephron model and urinalysis experiment 教室大小肾元模型的研制及尿液分析实验
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引用次数: 0
Bridging optogenetics, metabolism, and animal behavior for student-driven inquiry 桥接光遗传学,代谢和动物行为为学生驱动的调查
Tawny Aguayo-Williams, Vaaragie Subramaniam, Doug Harrison, R. Cooper, B. Criswell
Rm 105 Taylor Education Bldg, 597 S. Upper St, Department of STEM Education, University of Kentucky, Lexington, KY 40406-0001, USA 675 Rose St., Department of Biology, University of Kentucky, Lexington, KY 405060225 USA Department of Secondary Education, West Chester Univ., West Chester, PA. 19383 (taguayowilliams@gmail.com; vsu226@uky.edu; RLCOOP1@uky.edu; dough@uky.edu; brett.criswell@gmail.com)
泰勒教育大楼105室,597 S. Upper St, STEM教育系,肯塔基州列克星敦市,肯塔基州40406-0001,美国肯塔基州列克星敦市,肯塔基州大学,生物系玫瑰街675号,肯塔基州405060225美国西切斯特大学,西切斯特,宾夕法尼亚州。19383 (taguayowilliams@gmail.com;vsu226@uky.edu;RLCOOP1@uky.edu;dough@uky.edu;brett.criswell@gmail.com)
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引用次数: 0
A Picture is Worth 1000 Words: Using Pictorial Expression Data in Bioinformatics Assignments 一张图片胜过千言万语:在生物信息学作业中使用图形表达数据
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引用次数: 0
Bringing an Online Lab to Life 将在线实验室带入生活
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引用次数: 0
Alternative Assessments for Online Teaching That Are Bulletproof (Cheat-resistant) 在线教学的替代评估是防弹的(防作弊)
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引用次数: 0
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Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference
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