Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference最新文献
This discussion is focused on some experiences of semester-long course projects. At Rochester Institute of Technology (RIT), the first year laboratory courses (150 students per semester) are semester-long projects. We have adapted the Small World Initiative project (antibiotics from soil bacteria) and have devised and adapted other projects as a lens to teach laboratory techniques and engage our beginning scientists in authentic inquiry. Examples include Daphnia ecotoxicology and Fall tree studies. This past year has been challenging with the COVID-19 pandemic and the need to adapt laboratory courses to include students who are learning online, as well as planning for a pivot to online for the entire class. At the University of Kentucky (UK), in a Jr/Sr level Animal Physiology Course with a lab (120 students per semester) and a neurophysiology lab (Sr level) with 16 students we have focused on a theme this year with the effect of bacterial induced sepsis and to focus in on the direct effect of endotoxin (Lipopolysaccharides, LPS) from gram-negative bacteria. This was approached as authentic scientific inquiry. The novel investigation appeared to stimulate student engagement and curiosity. In the neurophysiology class the project turned into a publication with all students as co-authors. In the animal physiology course, the topic was woven through the various bodily systems from the cell level to the whole body. What students found interesting is how little is known on the direct effects of LPS as compared to the downstream actions of cytokines. To bridge this across kingdoms, the effects of LPS on root growth in plants and sensitivity to subsequent exposure as a defense mechanism were compared. Firstpage
{"title":"Semester-long Projects","authors":"Katherine A. Sharp, Robin L. Cooper, Dawn Carter","doi":"10.37590/able.v42.art50","DOIUrl":"https://doi.org/10.37590/able.v42.art50","url":null,"abstract":"This discussion is focused on some experiences of semester-long course projects. At Rochester Institute of Technology (RIT), the first year laboratory courses (150 students per semester) are semester-long projects. We have adapted the Small World Initiative project (antibiotics from soil bacteria) and have devised and adapted other projects as a lens to teach laboratory techniques and engage our beginning scientists in authentic inquiry. Examples include Daphnia ecotoxicology and Fall tree studies. This past year has been challenging with the COVID-19 pandemic and the need to adapt laboratory courses to include students who are learning online, as well as planning for a pivot to online for the entire class. At the University of Kentucky (UK), in a Jr/Sr level Animal Physiology Course with a lab (120 students per semester) and a neurophysiology lab (Sr level) with 16 students we have focused on a theme this year with the effect of bacterial induced sepsis and to focus in on the direct effect of endotoxin (Lipopolysaccharides, LPS) from gram-negative bacteria. This was approached as authentic scientific inquiry. The novel investigation appeared to stimulate student engagement and curiosity. In the neurophysiology class the project turned into a publication with all students as co-authors. In the animal physiology course, the topic was woven through the various bodily systems from the cell level to the whole body. What students found interesting is how little is known on the direct effects of LPS as compared to the downstream actions of cytokines. To bridge this across kingdoms, the effects of LPS on root growth in plants and sensitivity to subsequent exposure as a defense mechanism were compared. Firstpage","PeriodicalId":72079,"journal":{"name":"Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77199801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeffrey M. Chalfant, R. Cooper, Tawny Aguayo-Williams, Lexie, Holtzclaw, Madison Loveless, Jennifer Wilson, Doug Harrison
Using the established rules of Mendel and others, predicting the outcome of genetic crosses in model organisms is a common exercise for college students. Frequently, one uses visible phenotypic markers such as curly wings, eye color, and abnormal bristles to identify genetic outcomes. Yet many genetically-based traits, such as behavioral and physiological characteristics, are not easily observed. To demonstrate that such traits can likewise display classical genetic inheritance, we utilized an optogenetic system in Drosophila to modify response to light. We utilized the inheritance of behavioral responses associated with light-activated channelrhodopsin in motor neurons and body wall muscles. The frequency of responsive animals was quantified over multiple generations beginning with two pure-breeding (homozygous) strains, each containing one of the two components needed to produce the light-sensitive proteins. The use of light-sensitive channels to examine the predicted genetic outcomes is an approach which can be used in teaching classical genetic principles using non-traditional phenotypes. Green fluorescent protein (GFP) can be expressed to illustrate which cells are expressing channel rhodopsin. This introduces concepts of transgenesis, genetically-modified organisms, and genetic contributions to behavior. In addition to basic dominant and recessive allelic relationships, the experiments introduce more complex genetic concepts, such as epistasis, gene expression and cellular diversity, as well as physiological and behavioral traits of animals. This module is presented in a variety of ways depending on equipment availability and can be used in a hybrid or remote format with data provided. Firstpage
{"title":"Revisiting Mendel: use of a Behavioral Assay to Examine Inheritance of Traits in Drosophila","authors":"Jeffrey M. Chalfant, R. Cooper, Tawny Aguayo-Williams, Lexie, Holtzclaw, Madison Loveless, Jennifer Wilson, Doug Harrison","doi":"10.37590/able.v42.art56","DOIUrl":"https://doi.org/10.37590/able.v42.art56","url":null,"abstract":"Using the established rules of Mendel and others, predicting the outcome of genetic crosses in model organisms is a common exercise for college students. Frequently, one uses visible phenotypic markers such as curly wings, eye color, and abnormal bristles to identify genetic outcomes. Yet many genetically-based traits, such as behavioral and physiological characteristics, are not easily observed. To demonstrate that such traits can likewise display classical genetic inheritance, we utilized an optogenetic system in Drosophila to modify response to light. We utilized the inheritance of behavioral responses associated with light-activated channelrhodopsin in motor neurons and body wall muscles. The frequency of responsive animals was quantified over multiple generations beginning with two pure-breeding (homozygous) strains, each containing one of the two components needed to produce the light-sensitive proteins. The use of light-sensitive channels to examine the predicted genetic outcomes is an approach which can be used in teaching classical genetic principles using non-traditional phenotypes. Green fluorescent protein (GFP) can be expressed to illustrate which cells are expressing channel rhodopsin. This introduces concepts of transgenesis, genetically-modified organisms, and genetic contributions to behavior. In addition to basic dominant and recessive allelic relationships, the experiments introduce more complex genetic concepts, such as epistasis, gene expression and cellular diversity, as well as physiological and behavioral traits of animals. This module is presented in a variety of ways depending on equipment availability and can be used in a hybrid or remote format with data provided. Firstpage","PeriodicalId":72079,"journal":{"name":"Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80581915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Virtual Labs: Exploring New Boundaries in Teaching Biology","authors":"","doi":"10.37590/able.v42.abs24","DOIUrl":"https://doi.org/10.37590/able.v42.abs24","url":null,"abstract":"","PeriodicalId":72079,"journal":{"name":"Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80778590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.37590/able.v42.extabs20
{"title":"Incorporating digitized natural history collections data into virtual ecology and evolution\u0000course‐based research experiences","authors":"","doi":"10.37590/able.v42.extabs20","DOIUrl":"https://doi.org/10.37590/able.v42.extabs20","url":null,"abstract":"","PeriodicalId":72079,"journal":{"name":"Advances in biology laboratory education : publication of the .... Conference of the Association For Biology Laboratory Education (ABLE). Association for Biology Laboratory Education. Workshop/Conference","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80203358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}