Objective: Prevention of sexually transmitted diseases is an essential factor in managing diseases in the human population and increasing individuals’ and society’s health levels. Thus to increase our insufficient knowledge about proper prevention methods, we performed this study to assess the risk factors of the incidence of genital warts (GW). Methods: In this case-control study, we enrolled 120 women and divided them into two groups. Sixty women with GW were set in the case group, and 60 healthy women were set in the control group. Afterwards, a questionnaire consisting of 13 questions regarding their life habituates was filled. The answers were analyzed using T-tests and chi-square test. Findings: There was a statistically significant correlation between the incidence of GW and age (P-value ≤ 0.001*), the years passed from first intercourse (P-value =0.02*), and the history of venereal diseases (P-value =0.03*). However, the correlation between this disease and smoking, use of contraceptive medications, use of barrier and gravidity methods, marriage, education, the job of the individual and her partner, and the type of intercourse was not statistically significant. Conclusion: It is interesting that although some factors may increase the risk of GW, some factors that one might reason should affect the prevalence of GW such as the use of barriers, marriage, and socioeconomics of the individuals had no significant effect on the prevalence of GW in this study.
{"title":"The Risk Factors Associated with the Incidence of Genital Warts in Iran","authors":"Sara Talebipour Nikoo, A. Lotfi","doi":"10.33696/genetics.2.013","DOIUrl":"https://doi.org/10.33696/genetics.2.013","url":null,"abstract":"Objective: Prevention of sexually transmitted diseases is an essential factor in managing diseases in the human population and increasing individuals’ and society’s health levels. Thus to increase our insufficient knowledge about proper prevention methods, we performed this study to assess the risk factors of the incidence of genital warts (GW).\u0000\u0000Methods: In this case-control study, we enrolled 120 women and divided them into two groups. Sixty women with GW were set in the case group, and 60 healthy women were set in the control group. Afterwards, a questionnaire consisting of 13 questions regarding their life habituates was filled. The answers were analyzed using T-tests and chi-square test. \u0000\u0000Findings: There was a statistically significant correlation between the incidence of GW and age (P-value ≤ 0.001*), the years passed from first intercourse (P-value =0.02*), and the history of venereal diseases (P-value =0.03*). However, the correlation between this disease and smoking, use of contraceptive medications, use of barrier and gravidity methods, marriage, education, the job of the individual and her partner, and the type of intercourse was not statistically significant.\u0000\u0000Conclusion: It is interesting that although some factors may increase the risk of GW, some factors that one might reason should affect the prevalence of GW such as the use of barriers, marriage, and socioeconomics of the individuals had no significant effect on the prevalence of GW in this study.","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78284827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. EL-Seedy, Mariam H. Botros, Guylène Page, V. Ladevèze
Human dopamine transporter gene (SLC6A3) is one of the neurotransmission genes that plays an important role in controlling the behavior and psychological reactions toward various physical activities. The aim of the present study is to evaluate the associations between SLC6A3 3’-UTR VNTR (3’VNTR) genotypes and eight personality traits in Egyptian athletes. One hundred athletes (50 males and 50 females) and one hundred non-athletes participated in this investigation either in individual or team games. The Freiburg Personality inventory-Revised questionnaire was applied to athletes to evaluate personality traits according to the scale, at a scheduled time, using descriptive analysis. Genotyping for SLC6A3 3’-UTR VNTR polymorphism in athletes was realized to detect the different genotypes by PCR amplifications using desired primers. Three different genotypes of the 3’VNTR polymorphism; namely (9/9), (9/10), (10/10) were identified. The 9/10 and 10/10 genotypes of SLC6A3 gene were significantly higher in players than those in the control group. There were no statistically significant differences in personality traits values among males and females. The Cronbach’s alpha stability coefficient for four items is higher than 0.7 suggesting that these items (Neuroticism, Aggressiveness, Depression and Excitability) have a good reliability and support the used personality dimensions for this investigation. Our findings revealed a significant association between the genotypes of 3’VNTR and the personality traits in Egyptian athletes. These data shed light on the role of SLC6A3 gene in athletes’ behavioral performance and could serve as the basis for further characterization in the large number of Egyptian athletes.
{"title":"Allelic Variants on SLC6A3 Neurotransmitter Gene and Their Relationship with Personality Traits Scales in Egyptian Athletes","authors":"A. EL-Seedy, Mariam H. Botros, Guylène Page, V. Ladevèze","doi":"10.33696/genetics.2.012","DOIUrl":"https://doi.org/10.33696/genetics.2.012","url":null,"abstract":"Human dopamine transporter gene (SLC6A3) is one of the neurotransmission genes that plays an important role in controlling the behavior and psychological reactions toward various physical activities. The aim of the present study is to evaluate the associations between SLC6A3 3’-UTR VNTR (3’VNTR) genotypes and eight personality traits in Egyptian athletes. One hundred athletes (50 males and 50 females) and one hundred non-athletes participated in this investigation either in individual or team games. The Freiburg Personality inventory-Revised questionnaire was applied to athletes to evaluate personality traits according to the scale, at a scheduled time, using descriptive analysis. Genotyping for SLC6A3 3’-UTR VNTR polymorphism in athletes was realized to detect the different genotypes by PCR amplifications using desired primers. Three different genotypes of the 3’VNTR polymorphism; namely (9/9), (9/10), (10/10) were identified. The 9/10 and 10/10 genotypes of SLC6A3 gene were significantly higher in players than those in the control group. There were no statistically significant differences in personality traits values among males and females. The Cronbach’s alpha stability coefficient for four items is higher than 0.7 suggesting that these items (Neuroticism, Aggressiveness, Depression and Excitability) have a good reliability and support the used personality dimensions for this investigation. Our findings revealed a significant association between the genotypes of 3’VNTR and the personality traits in Egyptian athletes. These data shed light on the role of SLC6A3 gene in athletes’ behavioral performance and could serve as the basis for further characterization in the large number of Egyptian athletes.","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87170821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adam A Mwakyoma, Benson R Kidenya, Caroline A Minja, Martha F Mushi, Alison Sandeman, Wilber Sabiti, Mathew T G Holden, Stephen E Mshana
Background: The dissemination of the extended spectrum β-lactamases (ESBL) producing E. coli poses a significant public health problem. Understanding the efficiency and frequency of horizontal gene transfer via conjugation of ESBL producing E. coli is imperative towards devising prevention and control measures. This study compared the frequencies and efficiencies of horizontal blaCTX-M gene transfer via conjugation among Escherichia coli isolates from urine and gastrointestinal tract (GIT) of patients with urinary tract infection (UTI), their animals and environment.
Methods: Horizontal blaCTX-M gene transfer via conjugation by a broth mating experiment was performed using 50 confirmed ESBL producing E. coli isolates as donors and Escherichia coli J53 (F-, met, pro, Azr), as the recipient. The transconjugants were detected and their frequencies and efficiencies of conjugation were measured and compared between ESBL producing E. coli isolates multi-sourced from urine, GIT, animals and environment. Antimicrobial susceptibility testing of all resulting transconjugants was performed. DNA was extracted from all transconjugants to confirm the presence and the acquisition of blaCTX-M gene.
Results: Out of 50 ESBL producing E. coli isolates harboring blaCTX-M gene, 37 (74.0%) successfully exercised horizontal gene transfer through conjugation. All transconjugants were confirmed phenotypically and genotypically by PCR. Of note, all of the isolates from environment 100.0% (7/7) performed conjugation, exhibiting the highest transfer efficiency, followed by isolates from urine and animals, with the conjugation transfer efficiency of 77.8% (14/18) and 76.1% (10/13), respectively. The isolates from the environment conjugated with a significant more efficiency than those from the GIT [Two-sample test of proportions; p-value = 0.0119]. The overall conjugation transfer frequencies ranged from 0.4 × 10-14 - 5.5 × 10-11 per donor cells with the highest median conjugation transfer frequency observed among isolates from animal (3.23 × 10-12 [IQR: 0.70 × 10-12 - 7.22 × 10-12]) followed by that of isolates from the environment (1.60 × 10-12 [IQR: 0.30 × 10-12 - 5.0 × 10-12]).
Conclusion: ESBL producing E. coli from human, animals and environment exercises horizontal blaCTX-M gene transfer efficiently with the highest occurrence among isolates from the environment and animals. The antimicrobial resistance control and prevention strategies should be widened up to explore strategies to prevent horizontal AMR gene transfer.
{"title":"Comparison of Horizontal <i>bla<sub>CTX-M</sub></i> Gene Transfer via Conjugation among Extended Spectrum β-Lactamases Producing <i>Escherichia coli</i> Isolates from Patients with Urinary Tract Infection, Their Animals, and Environment.","authors":"Adam A Mwakyoma, Benson R Kidenya, Caroline A Minja, Martha F Mushi, Alison Sandeman, Wilber Sabiti, Mathew T G Holden, Stephen E Mshana","doi":"10.33696/genetics.2.011","DOIUrl":"10.33696/genetics.2.011","url":null,"abstract":"<p><strong>Background: </strong>The dissemination of the extended spectrum β-lactamases (ESBL) producing <i>E</i>. <i>coli</i> poses a significant public health problem. Understanding the efficiency and frequency of horizontal gene transfer via conjugation of ESBL producing <i>E</i>. <i>coli</i> is imperative towards devising prevention and control measures. This study compared the frequencies and efficiencies of horizontal <i>bla<sub>CTX-M</sub></i> gene transfer via conjugation among <i>Escherichia coli</i> isolates from urine and gastrointestinal tract (GIT) of patients with urinary tract infection (UTI), their animals and environment.</p><p><strong>Methods: </strong>Horizontal <i>bla<sub>CTX-M</sub></i> gene transfer via conjugation by a broth mating experiment was performed using 50 confirmed ESBL producing <i>E</i>. <i>coli</i> isolates as donors and <i>Escherichia coli</i> J53 (F<sup>-</sup>, <i>met</i>, <i>pro</i>, Az<sup>r</sup>), as the recipient. The transconjugants were detected and their frequencies and efficiencies of conjugation were measured and compared between ESBL producing <i>E</i>. <i>coli</i> isolates multi-sourced from urine, GIT, animals and environment. Antimicrobial susceptibility testing of all resulting transconjugants was performed. DNA was extracted from all transconjugants to confirm the presence and the acquisition of <i>bla<sub>CTX-M</sub></i> gene.</p><p><strong>Results: </strong>Out of 50 ESBL producing <i>E</i>. <i>coli</i> isolates harboring <i>bla<sub>CTX-M</sub></i> gene, 37 (74.0%) successfully exercised horizontal gene transfer through conjugation. All transconjugants were confirmed phenotypically and genotypically by PCR. Of note, all of the isolates from environment 100.0% (7/7) performed conjugation, exhibiting the highest transfer efficiency, followed by isolates from urine and animals, with the conjugation transfer efficiency of 77.8% (14/18) and 76.1% (10/13), respectively. The isolates from the environment conjugated with a significant more efficiency than those from the GIT [Two-sample test of proportions; p-value = 0.0119]. The overall conjugation transfer frequencies ranged from 0.4 × 10<sup>-14</sup> - 5.5 × 10<sup>-11</sup> per donor cells with the highest median conjugation transfer frequency observed among isolates from animal (3.23 × 10<sup>-12</sup> [IQR: 0.70 × 10<sup>-12</sup> - 7.22 × 10<sup>-12</sup>]) followed by that of isolates from the environment (1.60 × 10<sup>-12</sup> [IQR: 0.30 × 10<sup>-12</sup> - 5.0 × 10<sup>-12</sup>]).</p><p><strong>Conclusion: </strong>ESBL producing <i>E. coli</i> from human, animals and environment exercises horizontal <i>bla<sub>CTX-M</sub></i> gene transfer efficiently with the highest occurrence among isolates from the environment and animals. The antimicrobial resistance control and prevention strategies should be widened up to explore strategies to prevent horizontal AMR gene transfer.</p>","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9916984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Commentary on “Type II Toxin-antitoxin Systems Have a Peculiar Localization in Escherichia Coli Cells","authors":"","doi":"10.33696/genetics.1.009","DOIUrl":"https://doi.org/10.33696/genetics.1.009","url":null,"abstract":"","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88999202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Sleep on Autophagy and Neurodegenerative Disease: Sleeping Your Mind Clear","authors":"","doi":"10.33696/genetics.1.007","DOIUrl":"https://doi.org/10.33696/genetics.1.007","url":null,"abstract":"","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75225500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Actors of ROS Homeostasis in Stigmatic Cells Essential for Plant Reproduction","authors":"","doi":"10.33696/genetics.1.006","DOIUrl":"https://doi.org/10.33696/genetics.1.006","url":null,"abstract":"","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90279306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SARS-CoV-2 Mutations, Diagnosis and Their Concern","authors":"","doi":"10.33696/genetics.1.008","DOIUrl":"https://doi.org/10.33696/genetics.1.008","url":null,"abstract":"","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80004244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Risk Management Implemented by Turkey during the COVID-19 Pandemic Disaster","authors":"","doi":"10.33696/genetics.1.010","DOIUrl":"https://doi.org/10.33696/genetics.1.010","url":null,"abstract":"","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80172628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Analysis of early [1] and current [2] data on epidemiology and genetics of inherited developmental anomalies and other disorders allows us to note an interesting and important fact that among the various detected genetic changes, point mutations underlie almost one-half of the recessive Mendelian diseases [1] circulating in modern human populations. At the same time, the results of molecular analysis showed that the DNA changes underlying these mutations are represented mainly by base substitutions, indels, extended deletions or insertions and duplications [3-5]. Taking into account the well-known and important fact that the most dangerous mutagen for human is ionizing radiation with which humans are increasingly exposed on Earth (nuclear power station, radiotherapy, neutron research, nuclear disaster etc.) and in outer space. Therefore, it is important to know: (i) does ionizing radiation induces point mutations in germ cells in general, (ii) if so, what is the efficiency of sparsely and densely ionizing radiation in induction of such mutations, and (iii) what DNA changes underlie these mutations.
{"title":"The Nature of Radiation-induced Inherited Recessive Gene Mutations in Drosophila Melanogaster","authors":"I. Alexandrov, M. Alexandrova, K. Afanasyeva","doi":"10.33696/genetics.1.003","DOIUrl":"https://doi.org/10.33696/genetics.1.003","url":null,"abstract":"Analysis of early [1] and current [2] data on epidemiology and genetics of inherited developmental anomalies and other disorders allows us to note an interesting and important fact that among the various detected genetic changes, point mutations underlie almost one-half of the recessive Mendelian diseases [1] circulating in modern human populations. At the same time, the results of molecular analysis showed that the DNA changes underlying these mutations are represented mainly by base substitutions, indels, extended deletions or insertions and duplications [3-5]. Taking into account the well-known and important fact that the most dangerous mutagen for human is ionizing radiation with which humans are increasingly exposed on Earth (nuclear power station, radiotherapy, neutron research, nuclear disaster etc.) and in outer space. Therefore, it is important to know: (i) does ionizing radiation induces point mutations in germ cells in general, (ii) if so, what is the efficiency of sparsely and densely ionizing radiation in induction of such mutations, and (iii) what DNA changes underlie these mutations.","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85855822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Constitutively Active Death Receptor Induces Apoptosis in Mammalian Cells","authors":"","doi":"10.33696/genetics.1.004","DOIUrl":"https://doi.org/10.33696/genetics.1.004","url":null,"abstract":"","PeriodicalId":72286,"journal":{"name":"Archives of molecular biology and genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84846121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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