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Current problems in clinical biochemistry最新文献

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The difference in insulin sensitivity and metabolic response to acute exercise in trained and sedentary subjects. 胰岛素敏感性和代谢反应的差异在训练和久坐的受试者急性运动。
Current problems in clinical biochemistry
Pub Date : 1982-01-01
V A Koivisto, R DeFronzo, R Hendler, P Felig
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引用次数: 0
Peripheral insulinaemia and the glucoregulatory response to exercise in diabetic man. 外周胰岛素血症和糖尿病患者运动后的血糖调节反应。
Current problems in clinical biochemistry
Pub Date : 1982-01-01
B Zinman, E B Marliss, M Vranic
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引用次数: 0
Activation of platelet in vivo function and plasma levels of catecholamines and growth hormone during bicycle exercise in juvenile diabetes and healthy individuals. 青少年糖尿病和健康人自行车运动期间血小板体内功能的激活及血浆儿茶酚胺和生长激素水平的变化
Current problems in clinical biochemistry
Pub Date : 1982-01-01
G Schernthaner, I Mühlhauser, C Seebacher, H Templ, H Sinzinger, K Silberbauer
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引用次数: 0
The philosophy of exercise. 锻炼的哲学。
Current problems in clinical biochemistry
Pub Date : 1982-01-01
G Merikas
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引用次数: 0
Substrate metabolism during exercise in normal and diabetic man. 正常人和糖尿病人运动时底物代谢的研究。
Current problems in clinical biochemistry
Pub Date : 1982-01-01
J Wahren
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引用次数: 0
Physical training and cardiovascular disease in the diabetic. 体育锻炼与糖尿病患者心血管疾病的关系。
Current problems in clinical biochemistry
Pub Date : 1982-01-01
N B Ruderman, S Schneider, D Kramsch
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引用次数: 0
Nutrition and enzyme regulation. 营养和酶的调节。
Current problems in clinical biochemistry
Pub Date : 1980-01-01
H E Aebi, E G Berger
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引用次数: 0
Uromucoid excretion in normal individuals and stone formers. 正常人和结石患者的尿黏液排泄。
Current problems in clinical biochemistry
Pub Date : 1979-01-01
K H Bichler, V Ideler, R Harzmann

A quantitative immunological determination of uromucoid according to Laurell's method has been established. The average excretion rate of this glycoprotein in normal individuals was found to be 51.1 +/- 5 mg/24 hours. The uromucoid excretion in patients with calcium-oxalate, calcium-phosphate and cystine stones showed no significant change. In patients with urate stones and those with renal tubular acidosis a significant decrease in uromucoid excretion was found. Localization of uromucoid was found to be in the cells of the distal tubular section and the collecting tubules which was verified by immunincubation with FITC conjugated antihumanuromucoid globuline.

根据劳雷尔法，建立了尿黏液定量免疫学测定方法。正常人的平均排泄率为51.1 +/- 5mg /24小时。草酸钙、磷酸钙和胱氨酸结石患者的尿黏液排泄无明显变化。尿酸结石患者和肾小管酸中毒患者尿黏液排泄明显减少。经FITC偶联抗人黏液球蛋白免疫孵育证实，尿黏液定位于远端小管切片和集合小管细胞。
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引用次数: 0
Localisation of urinary tract infection by fraction 5 of lactic dehydrogenase. 乳酸脱氢酶5分数对尿路感染的定位。
Current problems in clinical biochemistry
Pub Date : 1979-01-01
U C Dubach
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引用次数: 0
Urinary N-acetyl-beta-D-glucosaminidase (NAG) as an indicator of renal disease. 尿n -乙酰- β - d -氨基葡萄糖酶(NAG)作为肾脏疾病的指标。
Current problems in clinical biochemistry
Pub Date : 1979-01-01
R G Price

The automated fluorimetric assay of N-acetyl-beta-D-glucosaminidase (NAG) has proved to be of value in the detection of rejection in transplant patients and in monitoring the course of renal disease. The urine can be diluted prior to assay, due to the sensitivity of the fluorimetric method and this reduces the effect of endogenous inhibitors. Time consuming dialysis or gel filtration steps are therefore unnecessary. Results are available to the physician within hours of the collection of the urine sample, since factoring the enzyme activity by the creatinine concentration avoids the necessity of collecting twenty-four hour or timed urine samples. Over one hundred samples can be assayed in a day using the automated procedure. The excretion of NAG in normal individuals varies with age and the activity found in pathological samples should always be compared with age-matched controls. The development of a new series of colorimetric substrates has allowed the production of a simple 'Dipstick' which should be of value in the screening of at-risk populations for renal disease. The diagnostic potential of the estimation of urinary NAG activity is enhanced by the separation of its isoenzymic forms by ion-exchange chromatography on DEAE-cellulose.

自动荧光法测定n -乙酰- β - d -氨基葡萄糖苷酶(NAG)已被证明是有价值的检测排斥在移植患者和监测肾脏疾病的进程。由于荧光法的敏感性，可以在测定前稀释尿液，这样可以减少内源性抑制剂的影响。因此，不需要耗时的透析或凝胶过滤步骤。结果可在收集尿样数小时内提供给医生，因为通过肌酐浓度分解酶活性避免了收集24小时或定时尿样的必要性。使用自动化程序，一天可以分析一百多个样品。正常个体的NAG排泄随年龄变化，病理样本中发现的活性应始终与年龄匹配的对照组进行比较。开发了一系列新的比色底物，可以生产一种简单的“量尺”，这在筛选肾脏疾病高危人群中应该是有价值的。通过离子交换色谱在deae -纤维素上分离其同工酶形式，提高了尿NAG活性估计的诊断潜力。
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引用次数: 0
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