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Discovery of MicroRNAs in Cardamom (Elettaria cardamomum Maton) under Drought Stress 干旱胁迫下小豆蔻microrna的发现
Pub Date : 2017-07-31 DOI: 10.1155/2017/9507485
N. Anjali, F. Nadiya, Jinu Thomas, K. Sabu
MicroRNAs (miRNAs) are endogenous, 19–24-nucleotide-long, noncoding RNAs found in animals, plants, and some viruses. Drought is one of the major limiting factors that negatively affect productivity of plants. To date, nothing is known about the regulatory roles of miRNAs in response to drought stress in cardamom. Cardamom collected from the natural forest area in Therakkudi in the Idamalayar forest range was selected for the study. Total RNA was isolated from leaves and stems of control and drought treated cardamom plant, which was used for library construction. Ion torrent sequencing of the two small RNA libraries prepared from plants raised under well irrigated and drought stressed treatments, respectively, created 3,938,342 and 4,083,181 primary reads. A total of 150 conserved and 20 novel microRNAs were identified from both the control and the treated libraries. Discovery of 17 differentially expressed miRNAs under drought stress suggests that these might have been involved in various biological processes to improve plant tolerance to water stress. This study is the first report of drought responsive miRNAs and their targets in cardamom. The outcome of this research could provide insights into the miRNA based regulatory mechanisms in response to drought stress in monocot plants.
MicroRNAs (miRNAs)是内源性的19 - 24个核苷酸长的非编码rna,存在于动物、植物和一些病毒中。干旱是影响植物生产力的主要限制因素之一。迄今为止,关于mirna在豆蔻干旱胁迫下的调节作用尚不清楚。本研究选取了在Idamalayar森林范围Therakkudi天然林地区采集的豆蔻。从对照和干旱处理的豆蔻植株叶片和茎中分离总RNA,用于文库建设。离子流测序分别从灌溉和干旱处理下的植物中制备了两个小RNA文库,分别产生了3,938,342和4,083,181个初级reads。从对照和处理过的文库中共鉴定出150个保守microrna和20个新的microrna。在干旱胁迫下发现了17个差异表达的mirna,这表明这些mirna可能参与了提高植物对水分胁迫耐受性的各种生物过程。本研究首次报道了豆蔻干旱响应mirna及其靶点。本研究结果为单叶植物干旱胁迫响应中基于miRNA的调控机制提供了新的思路。
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引用次数: 9
Anopheles gambiae: Metabolomic Profiles in Sugar-Fed, Blood-Fed, and Plasmodium falciparum-Infected Midgut 冈比亚按蚊:糖饲、血饲和恶性疟原虫感染中肠的代谢组学特征
Pub Date : 2017-07-24 DOI: 10.1155/2017/8091749
Cody J. Champion, Phanidhar Kukutla, Elizabeth K. K. Glennon, Bo Wang, S. Luckhart, Jiannong Xu
The mosquito midgut is a physiological organ essential for nutrient acquisition as well as an interface that encounters various mosquito-borne pathogens. Metabolomic characterization would reveal biochemical fingerprints that are generated by various cellular processes. The metabolite profiles of the mosquito midgut will provide an overview of the biochemical events in both physiological states and the dynamic responses to pathogen infections. In this study, the midgut metabolic profiles of Anopheles gambiae mosquitoes following feeding with sugar, human blood, mouse blood, and Plasmodium falciparum-infected human blood were examined. A mass spectrometry system coupled to liquid and gas chromatography produced a time series of metabolites in the midgut at discrete conditions (sugar feeding, 24 h and 48 h post-normal blood and P. falciparum-infected blood feeding). Triplicates were included to ensure system validity. A total of 512 individual compounds were identified; 511 were assigned to 8 superpathways and 75 subpathways. The dataset can be used for further inquiry into the metabolic dynamics of sugar and blood digestion and of malaria parasite infection. The dataset is accessible at the repository Dryad.
蚊子中肠是获取营养物质必不可少的生理器官,也是接触各种蚊媒病原体的界面。代谢组学表征将揭示由各种细胞过程产生的生化指纹。蚊中肠代谢谱将为蚊在生理状态下的生化事件和对病原体感染的动态反应提供一个概述。本研究检测了冈比亚按蚊摄食糖、人血、小鼠血和感染恶性疟原虫的人血后的中肠代谢谱。结合液相和气相色谱的质谱系统在离散条件下(糖摄食、24小时和48小时后正常血液和恶性疟原虫感染血液摄食)产生了中肠代谢物的时间序列。包括三份副本以确保系统有效性。共鉴定出512个单独的化合物;511人被分配到8个超通路和75个亚通路。该数据集可用于进一步研究糖和血液消化的代谢动力学以及疟疾寄生虫感染。该数据集可在存储库Dryad上访问。
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引用次数: 8
Five-Coordinate Zinc(II) Complex: Synthesis, Characterization, Molecular Structure, and Antibacterial Activities of Bis-[(E)-2-hydroxy-N′- {1-(4-methoxyphenyl)ethylidene}benzohydrazido]dimethylsulfoxidezinc(II) Complex 五配位锌(II)配合物:双-[(E)-2-羟基- n ' -{1-(4-甲氧基苯基)乙基}苯并肼]二甲基亚砜锌(II)配合物的合成、表征、分子结构和抗菌活性
Pub Date : 2017-06-15 DOI: 10.1155/2017/7520640
Zhi‐Qiang Liu, Yoke Mooi Ng, P. Tiong, Ruwaida Asyikin Abu Talip, Nornadia Jasin, V. Jong, M. Tay
The titled Zn(II) complex was synthesized by reacting the compound (E)-2-hydroxy-N′- 1-(4-methoxyphenyl)ethylidene benzohydrazide with zinc(II) acetate dihydrate in alkaline DMSO and ethanol solution under reflux condition for 28 hours. The resulting solid was filtered and recrystallized from the mixture of ethanol and DMSO. The hydrazone Schiff base and its Zn(II) complex were characterized using 1H, 13C NMR, FTIR, UV-Vis spectroscopy, and single crystal X-ray diffraction analysis. Meanwhile, their antibacterial activities were examined using disc diffusion method. The spectral studies showed that the hydrazone Schiff base underwent keto-enol tautomerization, forming a bidentate ligand (N,O) towards Zn(II) ion. Surprisingly, on top of the two hydrazone Schiff base molecules which coordinated to the Zn metal center, an additional DMSO molecule was found attached to the Zn metal center in the crystal data, resulting in a 5-coordinate distorted trigonal bipyramidal Zn(II) complex. Both hydrazone Schiff base and its Zn(II) complexes were found to exhibit low antibacterial activity even when the concentrations were increased to 800 ppm.
以(E)-2-羟基- n′- 1-(4-甲氧基苯基)乙基苯并肼与二水合乙酸锌在碱性二甲基亚砜和乙醇溶液中回流28h,合成了Zn(II)配合物。所得固体经过过滤,从乙醇和二甲基亚砜的混合物中再结晶。采用1H、13C NMR、FTIR、UV-Vis光谱和单晶x射线衍射等方法对腙希夫碱及其Zn(II)配合物进行了表征。同时采用圆盘扩散法对其抑菌活性进行了检测。光谱研究表明,腙希夫碱对Zn(II)离子发生了酮烯互变异构反应,形成双齿配体(N,O)。令人惊讶的是,在两个与Zn金属中心配位的腙希夫碱分子之上,在晶体数据中发现了一个附加的DMSO分子附着在Zn金属中心上,形成了一个5坐标畸变的三角双锥体Zn(II)配合物。腙希夫碱及其配合物Zn(II)在浓度达到800ppm时仍表现出较低的抗菌活性。
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引用次数: 11
Effect of Glyphosate and Mancozeb on the Rhizobia Isolated from Nodules of Vicia faba L. and on Their N2-Fixation, North Showa, Amhara Regional State, Ethiopia 草甘膦和代森锰锌对埃塞俄比亚阿姆哈拉州北昭和蚕豆根瘤菌及其固氮作用的影响
Pub Date : 2017-06-13 DOI: 10.1155/2017/5864598
Birhan Aynalem, F. Assefa
This study was designed to assess the effect of glyphosate and mancozeb on growth of Vicia faba rhizobia isolates in vitro and on their N2-fixation performance. Hence, ten isolates were isolated using plant-soil trap method from soil samples collected from farm lands. Those isolates were morphologically characterized using YEMA medium and authenticated as nodulating rhizobia using sand culture. These isolates were treated with 100, 150, and 200 μg a.e. L−1 glyphosate, 100, 150, and 200 mg L−1 mancozeb, and their combinations. The result showed that almost all isolates were affected (only 4–10% survival) at lower (100 mg L−1) concentration of mancozeb. However, 80% of isolates treated with higher concentration (200 μg a.e. L−1) of glyphosate for 72 h formed colonies on YEMA medium. Moderate (40%) isolates also showed better (31–50% and 17–45%) survival within 100 : 100 and 150 : 150 combinations of glyphosate and mancozeb, respectively. For in vivo experiment, faba bean seedlings in sand culture were inoculated with four relatively in vitro test resistant and one sensitive isolates. The inoculated isolates were treated with field recommended concentration of glyphosate, mancozeb, and combinations. Thus, experimental plants almost all showed normal (61–124 nodule plant−1) nodulation and N2-fixation (90–109%) performance as compared to the control.
本试验旨在研究草甘膦和代森锰锌对蚕豆根瘤菌体外生长和固氮性能的影响。采用植物-土壤陷阱法从农田土壤样品中分离出10株分离物。用叶马培养基对这些菌株进行形态鉴定,用沙培养鉴定为根瘤菌。这些分离株分别用100、150和200 μg a.e L - 1草甘膦、100、150和200 mg L - 1代森锰锌及其组合处理。结果表明,低浓度(100 mg L−1)的代森锰锌对几乎所有菌株都有影响(只有4-10%的存活率)。然而,高浓度(200 μg a.e L−1)草甘膦处理72 h, 80%的分离株在YEMA培养基上形成菌落。中度分离株(40%)在100:100和150:150的草甘膦和代森锰锌组合中也分别表现出更好的存活率(31-50%和17-45%)。在体内试验中,用4个相对体外试验抗性菌株和1个相对敏感菌株接种沙培蚕豆幼苗。接种的分离株用田间推荐浓度的草甘膦、代森锰锌和组合处理。因此,与对照相比,实验植株几乎都表现出正常的结瘤和固氮性能(90-109%)。
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引用次数: 13
Balancing African Elephant Conservation with Human Well-Being in Rombo Area, Tanzania 坦桑尼亚朗博地区非洲象保护与人类福祉的平衡
Pub Date : 2017-05-31 DOI: 10.1155/2017/4184261
N. Mmbaga, L. Munishi, A. Treydte
The critical assessment of the nature and extent of human-elephant conflict (HEC) and its impact on conservation efforts are essential if we are to meet the challenges related to extinction of local population, as well as loss biodiversity. Conservationists need detailed information on HEC in areas where these challenges prevail to improve intervention in the face of limited funds/resources. We assessed the status of HEC at Rombo area over the last six years. Data based on household surveys, focus group discussions, spatiotemporal analysis of site observations, and reported incidents of damage within the last six years were mapped. Out of all HEC cases analyzed, the most were crop damage which took place at night and the damage was severe between May and July, when cereal crops were mature. In upland areas of Rombo, HEC hotspots were observed inside the protected forest plantation where local people cultivated their annual crops. Cold spots concentrated in upland areas outside the protected forest plantation dominated by settlement and agroforestry less preferred by elephants. In lowland areas, HEC hotspots were observed in village lands close to the PAs, within settlement and farmland dominated by seasonal crops. This suggests that HEC management efforts such as establishment of buffer zones should be directed around the areas adjacent to PAs and prevention should focus on cultivation of the alternative crops and farming systems that are less preferred by elephants. Our study highlights the importance of using a combination of data collection techniques to pinpoint fine-scale HEC hotspots in a highly conflict-prone location of Tanzania.
如果我们要应对与当地人口灭绝和生物多样性丧失有关的挑战,对人象冲突(HEC)的性质和程度及其对保护工作的影响进行批判性评估至关重要。环保人士需要在这些挑战普遍存在的地区获得HEC的详细信息,以便在资金/资源有限的情况下改善干预措施。我们评估了过去六年来Rombo地区HEC的状况。基于住户调查、焦点小组讨论、现场观察的时空分析和过去六年内报告的损害事件的数据绘制了地图。在分析的所有HEC病例中,大多数是在夜间发生的作物损害,并且在谷类作物成熟的5月至7月期间损害严重。在Rombo的高地地区,在当地人种植一年生作物的保护林场内观察到HEC热点。寒区集中在受保护的人工林以外的高地地区,以定居和农林业为主,大象不太喜欢。在低地地区,HEC热点分布在靠近保护区的村庄土地、居民点和以季节性作物为主的农田内。这表明,HEC管理工作,如建立缓冲区,应围绕保护区附近的地区进行,预防工作应侧重于种植替代作物和大象不太喜欢的耕作系统。我们的研究强调了使用数据收集技术组合来确定坦桑尼亚高度冲突易发地区的小规模HEC热点的重要性。
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引用次数: 9
Observations of Unexpected Short-Term Heating in the Uppermost Layer of the Dead Sea after a Sharp Decrease in Solar Radiation 太阳辐射急剧减少后死海最上层意外短期升温的观测
Pub Date : 2017-05-25 DOI: 10.1155/2017/5810575
P. Kishcha, B. Starobinets, I. Gertman, T. Ozer, P. Alpert
The Dead Sea is one of the saltiest bodies of water in the world. Observational evidence has been obtained of unexpected short-term water heating in the 2 m uppermost layer of this hypersaline lake, following a sharp drop in solar radiation under weak winds. This was carried out using Dead Sea buoy measurements. Passing frontal cloudiness mixed with significant dust pollution over the Judean Mountains and the Dead Sea, which occurred on March 22, 2013, led to a dramatic drop in noon solar radiation from 860 W m−2 to 50 W m−2. This drop in solar radiation caused a short-term (1-hour) pronounced temperature rise in the uppermost layer of the sea down to 2 m depth. After the sharp drop in noon solar radiation, in the absence of water mixing, buoy measurements showed that the temperature rise in the uppermost layer of the Dead Sea took place for a shorter time and was more pronounced than the temperature rise under the regular diurnal solar cycle. The water heating could be explained by gravitational instability in the skin-surface layer, when the warm surface water with the increased salinity and density submerged, thereby increasing temperature in the layers below.
死海是世界上最咸的水体之一。观测证据表明,在弱风下太阳辐射急剧下降后,这个高盐湖最上层2米的地方出现了意想不到的短期水加热。这是使用死海浮标测量进行的。2013年3月22日,朱迪亚山脉和死海上空的锋面云混合了明显的沙尘污染,导致正午太阳辐射从860 W m−2急剧下降到50 W m−2。太阳辐射的减少导致海洋最上层2米深处的温度短期(1小时)明显上升。在正午太阳辐射急剧下降之后,在没有水混合的情况下,浮标测量表明,死海最上层的温度上升发生的时间较短,比正常的日太阳周期下的温度上升更为明显。水的加热可以用表层重力的不稳定性来解释,当盐度和密度增加的温暖的表层水浸入水中,从而提高了下面层的温度。
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引用次数: 2
Analysis and Comparison of Long-Distance Pipeline Failures 长输管道故障分析与比较
Pub Date : 2017-05-25 DOI: 10.1155/2017/3174636
L. Dai, Dong-po Wang, Ting Wang, Q. Feng, Xinqi Yang
The analysis results of long-distance oil and gas pipeline failures are important for the industry and can be the basis of risk analysis, integrity assessment, and management improvement for pipeline operators. Through analysis and comparison of the statistical results of the United States, Europe, the UK, and PetroChina in pipeline failure frequencies, causes, consequences, similarities, and differences of pipeline management, focusing points and management effectiveness are given. Suggestions on long-distance pipeline safety technology and management in China are proposed.
长输油气管道故障分析结果对油气行业具有重要意义,可以作为管道运营商进行风险分析、完整性评估和改进管理的依据。通过对美国、欧洲、英国和中石油在管道故障频次、原因、后果、管道管理异同等方面的统计结果进行分析比较,提出了管道管理的重点和管理效果。对国内长输管道安全技术与管理提出了建议。
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引用次数: 40
Development of a Sauce Using Gymnema sylvestre Leaves 匙羹藤叶酱料的研制
Pub Date : 2017-04-26 DOI: 10.1155/2017/5196524
O. Perera, P. Pavitha
Plant derived products have attracted enormous attention due to their diverse range of biological and therapeutic properties. Gymnema sylvestre is an underutilized medicinal herb that is widely distributed in different parts of the world. It is rich in phytochemicals with antidiabetic and antiobesity properties; however, it is less popular due to its bitter taste. This study was focused on developing a sauce using leaves of Gymnema sylvestre. The most acceptable composition (17.2% water, 4.8% vinegar, 3.2% sweet potato, 2.6% chili, 2.0% sugar, 2.0% cinnamon, 2.0% cardamom, 1.8% ginger, 1.8% garlic, 1.2% onion, 1.2% salt, 0.1% citric acid, and 0.1% sodium benzoate) of the sauce was with 60% leaf extract. Final product contains 73.54% ± 1.68 moisture, 19.92% ± 2.59 carbohydrate, 2.98% ± 0.44 ash, 2.61% ± 0.58 protein, 0.88% ± 0.05 fiber, and 0.05% ± 0.01 fat. The total flavonoid content was 243.67 ± 8.57 µ moles of rutin equivalent, total phenolic content was 65.44 ± 0.51 µ moles of gallic acid equivalent, and % of inhibition of DPPH of total antioxidant capacity was 43.70 ± 0.72. Sauce formulated with 1000 ppm sodium benzoate had six weeks of shelf life. The results showed that development of a sauce from G. sylvestre leaves is an effective way of delivering the health benefits to the potential consumers.
植物衍生产品由于其广泛的生物学和治疗特性而引起了人们的极大关注。Gymnema sylvestre是一种未被充分利用的草药,广泛分布在世界各地。含有丰富的植物化学物质,具有抗糖尿病和抗肥胖的特性;然而,由于它的苦味,它不太受欢迎。本研究的重点是开发一种酱汁利用匙羹叶。最可接受的酱油成分(17.2%水、4.8%醋、3.2%红薯、2.6%辣椒、2.0%糖、2.0%肉桂、2.0%豆蔻、1.8%姜、1.8%大蒜、1.2%洋葱、1.2%盐、0.1%柠檬酸、0.1%苯甲酸钠)为60%叶提取物。最终产品含水分73.54%±1.68,碳水化合物19.92%±2.59,灰分2.98%±0.44,蛋白质2.61%±0.58,纤维0.88%±0.05,脂肪0.05%±0.01。总黄酮含量为243.67±8.57 μ mol芦丁当量,总酚含量为65.44±0.51 μ mol没食子酸当量,抑制DPPH总抗氧化能力的%为43.70±0.72。以1000ppm苯甲酸钠配制的酱料保质期为6周。结果表明,从西洋参叶中开发酱料是向潜在消费者提供健康益处的有效途径。
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引用次数: 2
Complex Dynamics and Synchronization in a System of Magnetically Coupled Colpitts Oscillators 磁耦合Colpitts振子系统的复杂动力学与同步
Pub Date : 2017-04-10 DOI: 10.1155/2017/5483956
L. Kana, A. Fomethe, H. Fotsin, E. T. Wembe, A. Moukengue
We propose the use of a simple, cheap, and easy technique for the study of dynamic and synchronization of the coupled systems: effects of the magnetic coupling on the dynamics and of synchronization of two Colpitts oscillators (wireless interaction). We derive a smooth mathematical model to describe the dynamic system. The stability of the equilibrium states is investigated. The coupled system exhibits spectral characteristics such as chaos and hyperchaos in some parameter ranges of the coupling. The numerical exploration of the dynamics system reveals various bifurcations scenarios including period-doubling and interior crisis transitions to chaos. Moreover, various interesting dynamical phenomena such as transient chaos, coexistence of solution, and multistability (hysteresis) are observed when the magnetic coupling factor varies. Theoretical reasons for such phenomena are provided and experimentally confirmed with practical measurements in a wireless transfer.
我们建议使用一种简单、廉价和容易的技术来研究耦合系统的动态和同步:磁耦合对两个Colpitts振荡器的动态和同步的影响(无线交互)。我们推导了一个光滑的数学模型来描述动态系统。研究了平衡态的稳定性。耦合系统在耦合的某些参数范围内表现出混沌和超混沌等光谱特征。动力学系统的数值探索揭示了各种分岔情况,包括周期加倍和内部危机过渡到混沌。此外,当磁耦合系数变化时,观察到各种有趣的动力学现象,如瞬态混沌、解的共存和多稳定性(迟滞)。给出了这种现象的理论原因,并通过无线传输中的实际测量进行了实验证实。
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引用次数: 13
System Performance of an Inertially Stabilized Gimbal Platform with Friction, Resonance, and Vibration Effects 具有摩擦、共振和振动效应的惯性稳定万向节平台系统性能
Pub Date : 2017-03-28 DOI: 10.1155/2017/6594861
Ruting Jia, V. Nandikolla, G. Haggart, C. Volk, D. Tazartes
The research work evaluates the quality of the sensor to perform measurements and documents its effects on the performance of the system. It also evaluates if this performance changes due to the environments and other system parameters. These environments and parameters include vibration, system friction, structural resonance, and dynamic system input. The analysis is done by modeling a gimbal camera system that requires angular measurements from inertial sensors and gyros for stabilization. Overall, modeling includes models for four different types of gyros, the gimbal camera system, the drive motor, the motor rate control system, and the angle position control system. Models for friction, structural resonance, and vibration are analyzed, respectively. The system is simulated, for an ideal system, and then includes the more realistic environmental and system parameters. These simulations are run with each of the four types of gyros. The performance analysis depicts that for the ideal system; increasing gyro quality provides better system performance. However, when environmental and system parameters are introduced, this is no longer the case. There are even cases when lower quality sensors provide better performance than higher quality sensors.
研究工作评估传感器的质量来执行测量,并记录其对系统性能的影响。它还会评估该性能是否会因环境和其他系统参数而发生变化。这些环境和参数包括振动、系统摩擦、结构共振和动态系统输入。分析是通过建模一个云台相机系统,需要从惯性传感器和陀螺仪角度测量稳定。总体而言,建模包括四种不同类型的陀螺仪,万向相机系统,驱动电机,电机速率控制系统和角度位置控制系统的模型。分别分析了摩擦模型、结构共振模型和振动模型。对系统进行仿真,得到一个理想的系统,然后包括更现实的环境和系统参数。这些模拟是用四种陀螺仪中的每一种进行的。对理想系统进行了性能分析;提高陀螺仪质量,提高系统性能。然而,当引入环境和系统参数时,情况就不同了。甚至在某些情况下,低质量的传感器比高质量的传感器提供更好的性能。
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引用次数: 19
期刊
Adv. Artif. Neural Syst.
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