The incidence of Richter transformation in relapsed/refractory chronic lymphocytic leukemia (CLL) is up to 15% and Richter transformation is a frequent cause of treatment failure on ibrutinib (IBR) [1]. IBR has been promising in CLL cell growth suppression by inhibiting Bruton tyrosine kinase (BTK) [2]. However, resistance can occur due to specific mutations in BTK or PLCG2 genes [3]. Another agent, venetoclax (VEN) has shown excellent efficacy in relapsed refractory CLL [4]. In Richter transformation, the effectiveness of VEN alone or in combination with conventional chemotherapy has been demonstrated, although research in this area is limited and based on only a few cases [5-7]. BTK/PLCG2 mutations play a role in IBR-resistant CLL progression. However, there is limited knowledge about BTK or PLCG2 gene mutation statuses in Richter transformation.
A 72-year-old male was diagnosed with CLL characterized by a deletion in chromosome 17p [del(17p)] and a complex karyotype. After 12 months of diagnosis, he started IBR treatment. He initially showed a partial response. Two years after starting IBR, CLL cells in the peripheral blood increased, and he developed bilateral cervical lymphadenopathy, along with elevated levels of lactate dehydrogenase (LDH) and soluble interleukin-2 receptor (sIL-2R). Fluorodeoxyglucose-positron emission tomography (FDG-PET) revealed systemic lymphadenopathy. Bone marrow examination showed increased atypical lymphocytes, displaying a CD5+CD10-19+sIgκ+ phenotype, with del(17p) present in 27% of all nucleated cells, as confirmed by fluorescence in situ hybridization. The pathological examination of the cervical lymph nodes showed a diffuse proliferation of large lymphocytes that tested positive for CD5, CD19, CD20, CD22, CD23, CD45, and BCL-2 and negative for MUM-1 and BCL-6, with a Ki-67 index of ∼50%. A biopsy confirmed the transformation into diffuse large B-cell lymphoma (Figure 1). Genetic testing was conducted on lymph nodes and bone marrow samples collected simultaneously; TP53 mutation (R283P) was found in lymph nodes [variant allele frequency (VAF): 0.85] and bone marrow specimens (VAF: 0.77). However, PLCG2 mutation (D1144G) was only detected in the lymph node specimens (VAF: 0.07). In this case, a targeted gene panel was used in Japan. The sensitivity of allele mutation detection was 0.05. A single polymerase chain reaction was not performed. Additionally, we could not measure the IGHV mutation.
The patient's treatment involved VEN usage for gradually increasing doses up to 200 mg (with fluconazole concomitant use) as per guidelines, while the use of IBR gradually reduced (Figure 2). The patient's clinical symptoms (fever and others) and lymphadenopathy showed no signs of improvement with VEN initiation and IBR dose reduction. The laboratory values indicated a worseni
{"title":"Richter transformation acquiring PLCG2 mutation during Bruton tyrosine kinase inhibitors treatment","authors":"Takafumi Tsushima, Nobue Sato, Yong-Mei Guo, Hirotaka Nakamura, Kodai Kunisada, Song-Gi Chi, Kenta Akie, Yuki Takahashi, Saki Nakamura, Kaoru Shimada, Genichiro Ishii, Yosuke Minami, Junichiro Yuda","doi":"10.1002/jha2.891","DOIUrl":"10.1002/jha2.891","url":null,"abstract":"<p>The incidence of Richter transformation in relapsed/refractory chronic lymphocytic leukemia (CLL) is up to 15% and Richter transformation is a frequent cause of treatment failure on ibrutinib (IBR) [<span>1</span>]. IBR has been promising in CLL cell growth suppression by inhibiting Bruton tyrosine kinase (<i>BTK</i>) [<span>2</span>]. However, resistance can occur due to specific mutations in <i>BTK</i> or <i>PLCG2</i> genes [<span>3</span>]. Another agent, venetoclax (VEN) has shown excellent efficacy in relapsed refractory CLL [<span>4</span>]. In Richter transformation, the effectiveness of VEN alone or in combination with conventional chemotherapy has been demonstrated, although research in this area is limited and based on only a few cases [<span>5-7</span>]. <i>BTK</i>/<i>PLCG2</i> mutations play a role in IBR-resistant CLL progression. However, there is limited knowledge about <i>BTK</i> or <i>PLCG2</i> gene mutation statuses in Richter transformation.</p><p>A 72-year-old male was diagnosed with CLL characterized by a deletion in chromosome 17p [del(17p)] and a complex karyotype. After 12 months of diagnosis, he started IBR treatment. He initially showed a partial response. Two years after starting IBR, CLL cells in the peripheral blood increased, and he developed bilateral cervical lymphadenopathy, along with elevated levels of lactate dehydrogenase (LDH) and soluble interleukin-2 receptor (sIL-2R). Fluorodeoxyglucose-positron emission tomography (FDG-PET) revealed systemic lymphadenopathy. Bone marrow examination showed increased atypical lymphocytes, displaying a CD5+CD10-19+sIgκ+ phenotype, with del(17p) present in 27% of all nucleated cells, as confirmed by fluorescence in situ hybridization. The pathological examination of the cervical lymph nodes showed a diffuse proliferation of large lymphocytes that tested positive for CD5, CD19, CD20, CD22, CD23, CD45, and BCL-2 and negative for MUM-1 and BCL-6, with a Ki-67 index of ∼50%. A biopsy confirmed the transformation into diffuse large B-cell lymphoma (Figure 1). Genetic testing was conducted on lymph nodes and bone marrow samples collected simultaneously; <i>TP53</i> mutation (R283P) was found in lymph nodes [variant allele frequency (VAF): 0.85] and bone marrow specimens (VAF: 0.77). However, <i>PLCG2</i> mutation (D1144G) was only detected in the lymph node specimens (VAF: 0.07). In this case, a targeted gene panel was used in Japan. The sensitivity of allele mutation detection was 0.05. A single polymerase chain reaction was not performed. Additionally, we could not measure the <i>IGHV</i> mutation.</p><p>The patient's treatment involved VEN usage for gradually increasing doses up to 200 mg (with fluconazole concomitant use) as per guidelines, while the use of IBR gradually reduced (Figure 2). The patient's clinical symptoms (fever and others) and lymphadenopathy showed no signs of improvement with VEN initiation and IBR dose reduction. The laboratory values indicated a worseni","PeriodicalId":72883,"journal":{"name":"EJHaem","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jha2.891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140705807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Petra Bachanová, Joan How, Richard Dzeng, Sonia Mukherjee, Maia Pavlovic, Jennifer Lombardi, Gabriela Hobbs, Patrick M. Reeves
Myeloproliferative neoplasms (MPNs) are associated with immune dysregulation and increased susceptibility to infection, emphasizing the importance of vaccination for patients. This pilot study evaluated immune responses to influenza vaccination in MPN patients compared with healthy donors using mass cytometry and serology. We observed diminished CXCR5+ B-cell, CXCR3+ T-cell, activated CD127+ memory T-cell subsets, and a trend toward lower hemagglutinin inhibition titer in MPN patients. These results indicate that patients with MPN exhibit distinct responses to influenza vaccination suggestive of impaired migration to lymphoid organs and T-cell maturation which may impact the development of protective immunity.
骨髓增生性肿瘤 (MPN) 与免疫失调和感染易感性增高有关,因此强调了为患者接种疫苗的重要性。这项试验性研究使用质细胞计数法和血清学方法评估了 MPN 患者与健康供体相比对流感疫苗接种的免疫反应。我们观察到 MPN 患者的 CXCR5+ B 细胞、CXCR3+ T 细胞、活化的 CD127+ 记忆 T 细胞亚群减少,血凝素抑制滴度呈降低趋势。这些结果表明,MPN 患者对流感疫苗接种表现出不同的反应,这表明他们向淋巴器官的迁移和 T 细胞成熟受到了影响,可能会影响保护性免疫的发展。
{"title":"Immune profiling of responses to influenza vaccination in patients with myeloproliferative neoplasms","authors":"Petra Bachanová, Joan How, Richard Dzeng, Sonia Mukherjee, Maia Pavlovic, Jennifer Lombardi, Gabriela Hobbs, Patrick M. Reeves","doi":"10.1002/jha2.868","DOIUrl":"10.1002/jha2.868","url":null,"abstract":"<p>Myeloproliferative neoplasms (MPNs) are associated with immune dysregulation and increased susceptibility to infection, emphasizing the importance of vaccination for patients. This pilot study evaluated immune responses to influenza vaccination in MPN patients compared with healthy donors using mass cytometry and serology. We observed diminished CXCR5+ B-cell, CXCR3+ T-cell, activated CD127+ memory T-cell subsets, and a trend toward lower hemagglutinin inhibition titer in MPN patients. These results indicate that patients with MPN exhibit distinct responses to influenza vaccination suggestive of impaired migration to lymphoid organs and T-cell maturation which may impact the development of protective immunity.</p>","PeriodicalId":72883,"journal":{"name":"EJHaem","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jha2.868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140713229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuyang Lin, Bei Gao, Rui Xu, Hongming Shang, Yan Xiong, Jiayi Zhou, Zhe Yang, Chao Jiang, Shumei Yan
Numerous clinical studies speculated the association between multiple myeloma (MM) and inflammatory diseases; however, there is limited validation of these claims via establishing a causal relationship and revealing the underlying mechanism. This exploratory study employed bidirectional Mendelian randomization (MR) analysis to investigate the causal relationships between MM and inflammatory diseases, including atherosclerosis, asthma, ankylosing spondylitis, Alzheimer's disease (AD), Parkinson's disease (PD), sarcoidosis, inflammatory bowel disease, nonalcoholic fatty liver disease, type II diabetes, and schizophrenia (SZ). Transcriptomic and genome-wide Bayesian colocalization analyses were further applied to reveal the underlying mechanism. A significant and previously unrecognized positive association was identified between genetic predisposition to MM and the risk of SZ. Two independent case reports showed that treatment-resistant psychosis is due to underlying MM and is resolved by treating MM. From our MR analyses, various statistical methods confirmed this association without detecting heterogeneity or pleiotropy effects. Transcriptomic analysis revealed shared inflammation-relevant pathways in MM and SZ patients, suggesting inflammation as a potential pathophysiological mediator of MM's causal effect on SZ. Bayesian colocalization analysis identified rs9273086, which maps to the protein-coding region of HLA-DRB1, as a common risk variant for both MM and SZ. Polymorphism of the HLA-DRB1 allele has been implicated in AD and PD, further highlighting the impact of our results. Additionally, we confirmed that interleukin-6 (IL-6) is a risk factor for SZ through secondary MR, reinforcing the role of neuroinflammation in SZ etiology. Overall, our findings showed that genetic predisposition to MM, HLA-DRB1 polymorphism, and enhanced IL-6 signaling are associated with the increased risk of SZ, providing evidence for a causal role for neuroinflammation in SZ etiology.
许多临床研究推测多发性骨髓瘤(MM)与炎症性疾病之间存在关联;然而,通过建立因果关系和揭示内在机制来验证这些说法的研究却很有限。这项探索性研究采用了双向孟德尔随机化(MR)分析方法来研究多发性骨髓瘤与炎症性疾病(包括动脉粥样硬化、哮喘、强直性脊柱炎、阿尔茨海默病(AD)、帕金森病(PD)、肉样瘤病、炎症性肠病、非酒精性脂肪肝、II型糖尿病和精神分裂症(SZ))之间的因果关系。研究人员进一步应用转录组和全基因组贝叶斯共定位分析来揭示其潜在机制。研究发现,MM的遗传易感性与SZ的患病风险之间存在明显的正相关关系,而这一关系此前尚未得到认可。两份独立的病例报告显示,耐药性精神病是由潜在的 MM 引起的,并通过治疗 MM 而得到缓解。在我们的磁共振分析中,各种统计方法都证实了这一关联,而没有检测到异质性或多重效应。转录组分析发现,MM和SZ患者具有共同的炎症相关通路,这表明炎症是MM对SZ产生因果效应的潜在病理生理介导因素。贝叶斯共定位分析发现,映射到HLA-DRB1蛋白编码区的rs9273086是MM和SZ的共同风险变异。HLA-DRB1等位基因的多态性与AD和PD有关联,这进一步突出了我们研究结果的影响。此外,我们还证实白细胞介素-6(IL-6)是通过继发性MR导致SZ的一个风险因素,从而加强了神经炎症在SZ病因学中的作用。总之,我们的研究结果表明,MM的遗传易感性、HLA-DRB1多态性和IL-6信号的增强与SZ风险的增加有关,为神经炎症在SZ病因学中的因果作用提供了证据。
{"title":"Multiple myeloma, IL6, and risk of schizophrenia: A Mendelian randomization, transcriptome, and Bayesian colocalization study","authors":"Shuyang Lin, Bei Gao, Rui Xu, Hongming Shang, Yan Xiong, Jiayi Zhou, Zhe Yang, Chao Jiang, Shumei Yan","doi":"10.1002/jha2.890","DOIUrl":"10.1002/jha2.890","url":null,"abstract":"<p>Numerous clinical studies speculated the association between multiple myeloma (MM) and inflammatory diseases; however, there is limited validation of these claims via establishing a causal relationship and revealing the underlying mechanism. This exploratory study employed bidirectional Mendelian randomization (MR) analysis to investigate the causal relationships between MM and inflammatory diseases, including atherosclerosis, asthma, ankylosing spondylitis, Alzheimer's disease (AD), Parkinson's disease (PD), sarcoidosis, inflammatory bowel disease, nonalcoholic fatty liver disease, type II diabetes, and schizophrenia (SZ). Transcriptomic and genome-wide Bayesian colocalization analyses were further applied to reveal the underlying mechanism. A significant and previously unrecognized positive association was identified between genetic predisposition to MM and the risk of SZ. Two independent case reports showed that treatment-resistant psychosis is due to underlying MM and is resolved by treating MM. From our MR analyses, various statistical methods confirmed this association without detecting heterogeneity or pleiotropy effects. Transcriptomic analysis revealed shared inflammation-relevant pathways in MM and SZ patients, suggesting inflammation as a potential pathophysiological mediator of MM's causal effect on SZ. Bayesian colocalization analysis identified rs9273086, which maps to the protein-coding region of HLA-DRB1, as a common risk variant for both MM and SZ. Polymorphism of the HLA-DRB1 allele has been implicated in AD and PD, further highlighting the impact of our results. Additionally, we confirmed that interleukin-6 (IL-6) is a risk factor for SZ through secondary MR, reinforcing the role of neuroinflammation in SZ etiology. Overall, our findings showed that genetic predisposition to MM, HLA-DRB1 polymorphism, and enhanced IL-6 signaling are associated with the increased risk of SZ, providing evidence for a causal role for neuroinflammation in SZ etiology.</p>","PeriodicalId":72883,"journal":{"name":"EJHaem","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jha2.890","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140713728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Multiple myeloma (MM) is a disease, that at times poses diagnostic and monitoring challenges. Over the last decades laboratory methods have been expanded with serum free light chain (FLC) analysis. Alerted by two index cases with clinical impact due to failure of the FLC analysis to indicate a disease progression, we aimed to identify any clinical consequences due to known differences between FLC analysis methods. We applied two FLC analysis methods (Freelite Binding Site [FBS] and N-Latex Siemens [NLS]) on all patients with MM and monoclonal gammopathy of uncertain significance diagnosed/followed up at Södra Älvsborg Hematology Unit, from April to December 2022. From a total of 123 patients with malignant plasma cell disorder, we identified five cases (4.1%) where solely the FBS method, as opposed to NLS, urine and serum electrophoresis, could support diagnosis or detect progression. The consequences of this discrepancy included not only change of diagnosis or delayed therapy but also change of treatment. Our findings indicate that a stronger awareness of the potential weaknesses of different FLC methods is needed, which calls for a closer collaboration between clinical chemists and hematologists.
多发性骨髓瘤(MM)是一种有时会给诊断和监测带来挑战的疾病。过去几十年来,实验室方法已扩展到血清游离轻链(FLC)分析。由于 FLC 分析未能显示疾病的进展,有两个病例对临床产生了影响,因此我们希望找出 FLC 分析方法之间的已知差异对临床产生的任何影响。我们对 2022 年 4 月至 12 月期间在 Södra Älvsborg 血液科诊断/随访的所有 MM 和意义不确定的单克隆丙种球蛋白病患者采用了两种 FLC 分析方法(Freelite Binding Site [FBS] 和 N-Latex Siemens [NLS])。在总共123例恶性浆细胞病患者中,我们发现有5例患者(4.1%)仅通过FBS方法,而非NLS、尿液和血清电泳来支持诊断或检测病情进展。这种差异造成的后果不仅包括改变诊断或延迟治疗,还包括改变治疗方法。我们的研究结果表明,需要加强对不同 FLC 方法潜在弱点的认识,这就需要临床化学家和血液病学家之间更密切的合作。
{"title":"The choice of serum-free light chain analysis method could potentially have clinical consequences for myeloma patients","authors":"Ljupco Veskovski, Ingvar Jakobsson, Per-Ola Andersson, Therese Gustafsson, Annelie Sedigh, Dorota Knut-Bojanowska, Markus Hansson, Cecilie Hveding Blimark, Ulf-Henrik Mellqvist","doi":"10.1002/jha2.886","DOIUrl":"10.1002/jha2.886","url":null,"abstract":"<p>Multiple myeloma (MM) is a disease, that at times poses diagnostic and monitoring challenges. Over the last decades laboratory methods have been expanded with serum free light chain (FLC) analysis. Alerted by two index cases with clinical impact due to failure of the FLC analysis to indicate a disease progression, we aimed to identify any clinical consequences due to known differences between FLC analysis methods. We applied two FLC analysis methods (Freelite Binding Site [FBS] and N-Latex Siemens [NLS]) on all patients with MM and monoclonal gammopathy of uncertain significance diagnosed/followed up at Södra Älvsborg Hematology Unit, from April to December 2022. From a total of 123 patients with malignant plasma cell disorder, we identified five cases (4.1%) where solely the FBS method, as opposed to NLS, urine and serum electrophoresis, could support diagnosis or detect progression. The consequences of this discrepancy included not only change of diagnosis or delayed therapy but also change of treatment. Our findings indicate that a stronger awareness of the potential weaknesses of different FLC methods is needed, which calls for a closer collaboration between clinical chemists and hematologists.</p>","PeriodicalId":72883,"journal":{"name":"EJHaem","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jha2.886","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140721019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erica Franceschini, Valentina Menozzi, Vera Todisco, Mariachiara Pellegrino, Samuele Cantergiani, Andrea Dessilani, Anna Spadoni, Federico Romani, Alice Mazzocchi, Antonella Santoro, Marianna Meschiari, Adriana Cervo, Andrea Gilioli, Francesca Bettelli, Giulia Fregni-Serpini, Antonella Grottola, Anna Candoni, Giovanni Guaraldi, Mario Sarti, Mario Luppi, Cristina Mussini