European journal of biomedical research最新文献

{"title":"Bioinformatics Techniques for Developing Molecular Detection Methods for the HIV-1 Gag Gene","authors":"Asryadin Asryadin, Nilasari Indah Yuniati, Nur Aini Hidayah Khasanah, Adhi Aqwam, Rizka Khairunnisa, Hetti Koes Endang, Jumratul Nurhidayah, Daniel Djoko Wahyono, Alice Yuniaty","doi":"10.24018/ejbiomed.2024.3.4.97","DOIUrl":"https://doi.org/10.24018/ejbiomed.2024.3.4.97","url":null,"abstract":"The HIV-1 Gag gene, which plays an essential role in HIV replication, can be detected accurately using qRT-PCR. The quality of qRTPCR analysis is determined by the primers and probes used for DNA amplification. This research aims to use bioinformatics techniques to design primer pair sequences and qRT-PCR probes for HIV detection using the HIV-1 Gag gene. HIV-1 Gag gene sequences were obtained from HIV-1 isolates and serotypes, downloaded from the National Center for Biotechnology Information (NCBI) GenPeptd nucleotide database. Sequences were then examined using the ClustalW algorithm of the Bioedit sequence alignment editor version 7.2.5.0. through gene alignment using multiple sequence alignment (MSA) with conserved regions. The primer pair sequences of the Gag-HIV 1 gene were obtained, namely, forward 5'-CAGTACAATGTGCTTCCACAGGG-3 and reverse 3'-CGGGATAGAGATTCAGTCTAGG-5' with the probe sequence 5'-GGATCACCAGCAATATTTCAGGGAACG-3'. The primer sequence has a length of 23 bases (forward), 22 bases (reverse), GC content of 52% (reverse), 50% (forward), and the same forward and reverse melting temperature (Tm) of 66°C. The probe sequence is 27 bases long, with a GC content of 48% and a Tm of 67.3°C. No hairpin loops and dimers were formed in the primer pair or probe, and the gag gene had 100% homology with HIV-1. It was concluded that the primer and probe pair sequences met the requirements and could be used to amplify the HIV-1 Gag gene using qRT-PCR.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141807693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytomorphological Analysis of Liquid PRF Produced with DUO Fixed Angle Centrifuge (Process, France)","authors":"M. Crisci, Giovanni Lepore, Federica Feleppa, A. Crisci, Fabiana Flagiello","doi":"10.24018/ejbiomed.2024.3.1.82","DOIUrl":"https://doi.org/10.24018/ejbiomed.2024.3.1.82","url":null,"abstract":"Background: Liquid PRF is a second-generation injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by centrifugation of autologous blood.\u0000Methods: This study aims to analyze the cellular and Fibrinogen content of various types of Liquid PRF (C-PRF liquid, A-PRF liquid, i-PRF, Liquid Fibrinogen) obtained with the use of DUO Fixed Angle Centrifuge (PRF DUO, Process for PRF, Nice, France) with Vacumed FL tubes (code 44909) and/or original S-PRF Sticky tube. An average accumulation of thrombocytes of almost 1.5 times compared to whole blood was found. Due to the high concentration of platelets, PRF-Liquids contain important growth factors for tissue regeneration.\u0000Results: In this preliminary study, we have shown that the type of Liquid PRF with a higher content of Platelets (126.3% vs. 109.5%), Monocytes (127.6% vs. 84.6%), with a sufficient content of Lymphocytes (192.9% vs. 242.1%) and Neutrophilic Granulocytes (64.6% vs. 64.8%) and Fibrinogen (67.9% vs. 87.3%), is the i-PRF (700 rpm x 5') obtained with Vacumed FL tube (code 44909) with statistically insignificant differences compared to whole blood, while the content of Lymphocytes and Fibrinogen present in i-PRF (700 rpm x 5') obtained with PRF-S-Sticky tube is higher. In comparison, the content of cells and Fibrinogen obtained with the two methods of preparing PRP is much lower.\u0000Conclusions: This indicates that the liquid PRF is more suitable to be used in various cases of tissue regeneration such as facial aesthetics, intra-articular injection, peri-ulcerative injection, etc., is the i-PRF (700 rpm x 5') obtained with a Vacumed FL tube.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"32 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification of Sars-Cov2 Viral Markers in Côte d’Ivoire","authors":"A. Sylla, S. Kakou-ngazoa, B. K. Bla, Tata G. S. Coulibaly, Zeinab Ouattara, Y. K. Ouattara, A. Addablah, Mireille S. Kouamé-Sina, Venance Kouakou, D. N. Coulibaly, Mireille Dosso","doi":"10.24018/ejbiomed.2023.2.4.80","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.4.80","url":null,"abstract":"The COVID-19 pandemic is causing millions of deaths worldwide. In West Africa, particularly in Côte d’Ivoire, many cases of illness and death have also been reported. The country has applied the diagnostic method recommended by the World Health Organization based on the detection of the genetic material of the SARS-CoV2 virus using RT-qPCR, which requires native or recombinant positive controls to validate the diagnostics. Recombinant plasmids are used for in vitro amplification in E. coli strains. This study aims to propose a bank of recombinant viral genomes for the diagnosis of SARS-CoV-2.\u0000A total of fifty (50) positive nasopharyngeal samples have been collected during the pandemic from 2020 to 2022. ARN viral extraction was applied, and the viral targets of Envelope, Membrane, Nucleoprotein, and Glycoprotein Spike were amplified using RT-qPCR methods. The PCR products were cloned to obtain recombinant plasmids.\u0000Our results show positive amplification of the three genes E, M, and N detected with positive rates of 50%, 40%, and 36%. A partial 995 bp fragment of the S gene was amplified at a low rate. \u0000Recombinant plasmids of SARS-CoV-2 were obtained, 50, 40, and 13 for the E gene, M gene, and N gene respectively. The recombinant plasmids were detected positive by conventional PCR and real-time PCR of SARSCoV-2. The nucleotide sequence of recombinant plasmids shows sequences homologies of 95% to 100% with the Wuhan reference strain for all three genes. This study has provided reliable recombinant plasmids for the diagnosis of SARS-CoV-2 in Côte d’Ivoire and offers the way for future studies to diagnostics tools.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"114 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letrozole and HFD_HG on Insulin and GDP","authors":"Fitria Nurul Hidayah, Alfaina Wahyuni, Ana Majdawati","doi":"10.24018/ejbiomed.2023.2.4.77","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.4.77","url":null,"abstract":"The pathogenesis of Polycystic ovary syndrome (PCOS) involves several mechanisms, that include the disorders of carbohydrate and lipid metabolism, oxidative stress, and insulin resistance. That condition makes PCOS have a great impact on long-term health problems such as cardiovascular disease, atherosclerosis, and diabetes. What is the potential for increased diabetic in a PCOS model mouse? This study aims to determine the potential for diabetes cases in PCOS patients through observations in PCOS model mice. Research method: using Postest Only Control Group Design method. Samples included 24 female, 3 months aged rats Rattus norvegicus strain Wistar and weighing around 100–130 grams. After observing the estrous cycle, 16 rats were randomly selected to make a model PCOS with letrozol induction 1 mg/kg BW/day orally in combination with a high-fat high-fructose diet. The treatments were given for 18, 21, 24 and 27 days. Furthermore, after fasting for 12 hours, fasting sugar levels and insulin levels were measured using the ELISA method. The results were analyzed by One Way Anova test with a significance level of α = 0.05. Data analysis was conducted with the SPSS program version 24.0 for Windows. Results: There was no significant difference in fasting insulin levels between the control group and the PCOS group (p > 0.05), but fasting blood sugar levels increased significantly (p < 0.05). Conclusion: There was an increase in the diabetic potency of PCOS mice-induced by the combination of testosterone propionate and a high-fat, high-fructose diet even when insulin levels are not significantly increased.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"14 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139237577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaccination Rate for COVID-19 in a Primary Healthcare Center in Greece","authors":"M. Stamouli, Christina Seitopoulou, Georgia Kalliora, Nikoletta Renesi, A. Mourtzikou","doi":"10.24018/ejbiomed.2023.2.4.79","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.4.79","url":null,"abstract":"The novel coronavirus SARS-CoV-2 has been the contributing factor to the COVID-19 disease pandemic, since early 2020. It first appeared in December 2019 and since then rapidly spread worldwide. The severe symptoms of COVID-19 disease, especially in the population groups with higher risk of infection or disease complications, along with the increased mortality rates determined the need for adequate protection and prevention measures to be taken. From the beginning of the pandemic, efforts have been made to develop and produce vaccines that could stop the spread of the disease. The vaccination in most countries was performed in phases, with priority to population groups with a higher risk of infection or disease complications, such as the elderly and patients with chronic diseases, and to population groups with a high risk of professional exposure. This study presented data from the vaccination program, implemented at a primary healthcare center in Greece, that served as a vaccination center during the pandemic. Relying on the results, both the attendance rate and the vaccination rate were very high, which proves that the necessity of the vaccine is well established and that vaccination hesitancy is mitigated among the population served at the Nikea Health Center.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139273234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Administration of 2% Plum (Prunus domestica L.) Extract Cream Inhibited the Increase of Tyrosinase Enzyme Expression and the Amount of Skin Melanin in Male Guinea Pigs (Cavia porcellus) Skin Exposed to UV B Light","authors":"Mila Damayanti Wahyuningsih, W. Pangkahila, N. Winarti","doi":"10.24018/ejbiomed.2023.2.3.52","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.3.52","url":null,"abstract":"\u0000Medical science has progressed a lot and Anti Aging Medicine (AAM) is one of the sciences in the medical world that brings a new paradigm. Hyperpigmentation is one of the most common skin problems caused by excessive pigment melanin production. Plums are nutrient-rich and can be eaten raw, dried, or processed. This research aimed to evaluate the administration of 2% plum extract in inhibiting the increase of tyrosinase enzyme expression and the amount of skin melanin in male guinea pigs’ skin exposed to UV B light. This was a randomized post-test-only control group design using 30 male guinea pigs (Cavia porcellus), aged 12-16 weeks, weight 300-350 grams, and divided into three groups, the control group did not get any cream, treatment group 1 received cream base, and treatment group 2 got 2% plum extract cream. Creams were applied daily 0.1 mg/cm2 at 09.40 and 14.00 WITA. All guinea pigs received UV-B irradiation, three times a week with a total dose of 390 mJ/cm2. Tyrosinase enzyme expression was examined by calculating the percentage of cells expressing the enzyme using immunohistochemical methods. On the other hand, the amount of melanin was obtained by calculating the melanin percentage that appeared on histopathological preparations using Masson-Fontana staining. The data obtained were analyzed using the One-way ANOVA test with a significance value of p<0.05. The expression of the tyrosinase enzyme in the control group and group with base cream was not significantly different (22.75 ± 5.50% vs. 21.30 ± 6.50%, p = 0.601). Similarly, the mean amount of melanin (4.42 ± 1.57% vs 4.27 ± 1.63%, p = 0.775). On the other hand, the results of the mean tyrosinase expression and the mean amount of melanin in the group receiving 2% plum extract were significantly lower than the treatment group receiving base cream (7.06 ± 5.18% vs 21.30 ± 6.50% and 0.42 ± 0.19% vs. 4.27 ± 1.63%) with p-value < 0.000. It can be concluded that the administration of 2% plum (Prunus domestica L.) extract cream inhibited the increase in the expression of the tyrosinase enzyme and the amount of melanin in the skin of male guinea pigs (C. porcellus) exposed to UVB light.\u0000","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75969525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burdock Root (Arctium lappa) Reduces Steatosis and Serum Malondialdehyde in Wistar Rats Fed with Used Cooking Oil","authors":"J. Jessica, I. M. W. Ruma, I. G. A. Widianti","doi":"10.24018/ejbiomed.2023.2.3.67","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.3.67","url":null,"abstract":"Utilization of used cooking oil may cause fat accumulation in the body leading to exceeding metabolic capacity of the liver and lipid peroxidation, subsequently triggering oxidative stress that will lead to non-alcoholic fatty liver disease (NAFLD). Burdock root (Arctium lappa) have hypolipidemic, antioxidant, and anti-inflammatory properties. This study aims to determine the effects of Burdock root to reduce steatosis and malondialdehyde (MDA) plasma levels in male Wistar rats fed with used cooking oil.\u0000This study used a post-test only control group design. Thirty healthy male Wistar rats were randomized into three groups. All groups were given 0,42 mL of used cooking oil. Fifteen minutes after, the intervention group 0 (P0) was given 1 mL of distilled water 1x/day. The intervention group 1 (P1) was given 100 mg/kg BW of burdock roots ethanolic extract diluted in 1 mL of distilled water 1x/day. The intervention group 2 (P2) was given 200 mg/kg BW of burdock roots ethanolic extract diluted in 1 ml of distilled water 1x/day. After 28 days, histopathological examination of the liver tissue to measure steatosis and peripheral blood taken to measure serum MDA levels and compared between groups.\u0000The results showed that the average steatosis in the P0 group was significantly higher than the P1 group (15.51±3.22% vs. 8.92±1.49%, respectively; p = 0.00). Similar results between the P1 group were also significantly higher compared to the P2 group (5.18±1.31%; p = 0.002). The mean serum MDA level revealed a significantly higher results among the P0 group compared to P1 group as well as P1 group compared to P2 group (12.58±1.92 nmol/mL vs. 9.76±0.47 nmol/mL, respectively; p=0.011 and (9.76±0.47 nmol/mL vs. 8.69±0.33 nmol/mL, respectively; p=0.011).\u0000The conclusions of this study were that burdock roots could reduce steatosis and serum MDA in male Wistar rats that were given used cooking oil.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90551325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Respiratory Virus Circulation in a Tertiary Care Hospital in Greece","authors":"A. Mourtzikou, M. Stamouli, Elpida Toka, Panagiotis Koumpouros, Georgia Kalliora, Christina Seitopoulou, Maria Kimouli","doi":"10.24018/ejbiomed.2023.2.3.65","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.3.65","url":null,"abstract":"Background: The COVID-19 pandemic caused by the novel SARS-CoV-2 virus affected health care systems and public health worldwide dramatically. Several measures were applied in order to prevent or stop the rapid transmission of the virus and the subsequent disease, such as lockdowns, physical distancing, strictly hygiene, along with travel restrictions. Global population after vaccination programs against COVID-19 were carried out, is facing a “tripledemic” situation threat, with the co-existance of SARS-CoV-2, influenza and RSV. The aim of the present study was to evaluate the co-existence of SARS-CoV-2, influenza and RSV, as well as the correlation with gender, age, Cts and vaccination doses.\u0000Methods: A total of 302 patients were included in the study. All patients were admitted to the emergency department of General Hospital Nikea, Piraeus with common upper respiratory tract symptoms and were suspected for COVID-19 disease, between March to July 2022. Patients’ age, gender, vaccination doses, and results from RT-PCR detection for SARS-CoV-2, RSV and Influenza viruses were recorded.\u0000Results: 139 were male and 163 female, aged between 18-94 years. Out of the patients included in the study, 206 were vaccinated and 96 were not vaccinated. Among vaccinated patients 97 were male and 109 were female. A percentage of 3.3% had received one vaccination dose, 16.9% two and 47.7% three. Moreover, 88 patients presented infection symptoms; 81 patients had a positive rapid test result. We detected 15 cases of co-infection of SaRS-CoV-2 and RSV and only one case, of co-infection of SaRS-CoV-2 with influenza virus.\u0000Conclusions: The majority of patients admitted to the emergency department of GHNP with common upper respiratory tract clinical manifestations were female. A significantly lower rate of co-infection with SARS-CoV-2 and RSV was detected in patients having received 2 vaccination doses, compared to patients having received 3 out of 3 vaccination doses or up to 1 vaccination dose. Ct values for SARS-CoV-2 and RSV pathogens were between 10-17. Co-infection with SARS-CoV-2 and Influenza was detected in only 1 patient.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84909931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of CCND1 Gene Polymorphism rs9344 with Grade and Invasion Degree of Colorectal Cancer at Prof. Dr. I.G.N.G. Ngoerah Central General Hospital","authors":"Nanang Masrani, N. N. A. Dewi, N. Linawati, N. P. Ekawati, I. Mulyawan","doi":"10.24018/ejbiomed.2023.2.2.58","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.2.58","url":null,"abstract":"The majority of colorectal cancer (CRC) are sporadic CRC that can be caused by genetic variations such as Single Nucleotide Polymorphisms (SNPs). The CCND1 gene polymorphism rs9344 could involve at the beginning and the development of CRC.\u0000This study aimed to analyze the association between CCND1 gene polymorphism rs9344 with the grade and invasion degree of colorectal cancer in at Prof. Dr. I.G.N.G. Ngoerah Central General Hospital, Denpasar, Bali. This cross-sectional study was carried out at the Integrated Biomedical Laboratory Unit, Faculty of Medicine, Udayana University.\u0000Data analysis of 32 samples showed majority age was above 50 years old as many as 28 (87.5%), with men as 21 samples (65.6%). Histopathology description was adenocarcinoma in 32 samples (100%). Grading histopathology low grade was 31 samples (96.6%). The degree of tumor invasion was high as 25 samples (78.1%). From the aspect of pathological stage pNx as much as 27 (84.4%). The majority location of tumors was on the left side as 21 samples (65.6%). The polymorphisms of CCND1 rs9344 genotype sequentially AA as 20 samples (62.5%), AG as 9 samples (28.1%), while GG as 3 samples (9.4%). The statistical analysis found that CCND1 gene polymorphism rs9344 was not associated with colorectal cancer grade (p>0.05), and not associated with invasion degrees of colorectal cancer (p>0.05).\u0000Taken together, we conclude that no significant association between the CCND1 gene polymorphism rs9344 with grade and invasion degree of colorectal cancer at Prof. Dr. I.G.N.G. Ngoerah Central General Hospital, Denpasar, Bali.\u0000 ","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84972164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IL-10 A/G rs1800896 and TNF-α G308A rs1800629 Polymorphisms and Their Relationship with the Risk of Implant Loss in Adult Patients from Argentina","authors":"A. Bono, J. C. Ibáñez, M. Sezin, M. Brunotto","doi":"10.24018/ejbiomed.2023.2.2.59","DOIUrl":"https://doi.org/10.24018/ejbiomed.2023.2.2.59","url":null,"abstract":"Introduction: the results of treatment in implantology have been evaluated mainly as implant survival rates in small groups of patients selected from specialized clinical or university settings. There is evidence to support polymorphisms that could be linked to some biological complications in implantology. The results may vary according to the origin or ethnic mixtures of the population studied. The objective of this study was to analyze the relationship between the polymorphisms IL-10 A/G rs1800896 and TNF-α G308A rs1800629308 and the loss of dental implants and periodontal disease. Material and method: 140 patients were selected, 10 with implant losses within a maximum period of 6 months of their placement. Each patient voluntarily consented to participate in the study (approved by CIEIS Adult Hospital Córdoba) Filiatory and clinical data were collected in a clinical history. Samples of clinically healthy oral mucosa were obtained and genotyped by PCR and RFPL. Results: The population consisted of male and female individuals in similar proportions (p=0.6121). The average age was 53.06±16.22 years, and an age variation range of 20 to 80 years. 7.7% of the patients included in the study had loss of their implant. It was observed that 62.5% of the patients who presented loss of implants were women. Of the patients with missing implants, 75% were smokers and did not consume alcohol. On the other hand, in relation to periodontal disease only 31% presented disease. In relation to genotype, patients with lost implants had 50% of the mutated allele of the SNP TNFα rs1800629, while 50% of patients with periodontal disease were carriers of the mutated allele for SNP IL-10 rs1800896. On the other hand, considering the total population under study, 31.06% of the patients presented the genotypes with the genetic variation, AG+GG, of the IL10 rs1800896 gene; while 64.07% presented the AA and GA genotypes, (both with the mutated allele) of the TNF-α G308A rs1800629 gene. No significant association (p=0.3298) was observed between IL10 rs1800896 genotypes and periodontal disease; contrary to whether there was a significant relationship of this SNP with periodontal disease (p=0.0164). Conclusion: The polymorphisms evaluated were not predictive of the failure of dental implants. However, a significant association between periodontal disease and TNF-α rs1800629 genotype could be observed. It is noteworthy that this is the first study that describes the frequency of the SNPs studied in a population of Córdoba–Argentina.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":"123 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80057422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}