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Prospects for Fluorescence Molecular In Vivo Liquid Biopsy of Circulating Tumor Cells in Humans 人循环肿瘤细胞荧光分子活体液体活检的前景
Pub Date : 2022-05-09 DOI: 10.3389/fphot.2022.910035
M. Niedre
Our team recently developed “Diffuse in vivo Flow Cytometry” (DiFC) for detection and enumeration rare circulating tumor cells (CTCs) in mice with highly-scattered fluorescent light. We have used DiFC to study dissemination of CTCs in a number of mouse models of metastasis with fluorescent protein expressing cells. Because DiFC uses diffuse light and interrogates large blood vessels in relatively deep tissue, in principle it could be translated to larger limbs, species, and even humans clinically. In this perspective, we discuss the technical challenges of human translation of DiFC in the context of the current state of the technology, as well as potential strategies for labeling of CTCs with targeted fluorescent molecular probes. We also discuss potential advantages and disadvantages of DiFC as a clinical tool. In principle, DiFC could represent a powerful complementary technique (to liquid biopsy blood draws) for accurate and sensitive measurement of changes in CTC numbers over time.
我们的团队最近开发了“弥漫性体内流式细胞术”(DiFC),用于在高散射荧光灯下检测和枚举小鼠体内罕见的循环肿瘤细胞(ctc)。我们利用DiFC研究了CTCs在多种表达荧光蛋白的小鼠转移模型中的传播情况。由于DiFC使用漫射光,并在相对较深的组织中检查大血管,原则上它可以转化为更大的肢体,物种,甚至临床人类。从这个角度来看,我们讨论了在技术现状的背景下人工翻译DiFC的技术挑战,以及用靶向荧光分子探针标记ctc的潜在策略。我们还讨论了DiFC作为临床工具的潜在优点和缺点。原则上,DiFC可以代表一种强大的补充技术(液体活检血液),用于准确和敏感地测量CTC数量随时间的变化。
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引用次数: 4
Optical Simulation Study of Perovskite/CIGS Tandem Solar Cells With Reduced Graphene Oxide Layers 还原氧化石墨烯层钙钛矿/CIGS串联太阳能电池的光学模拟研究
Pub Date : 2022-05-04 DOI: 10.3389/fphot.2022.888486
M. Kovačič, J. Krč, K. Savva, S. Maragkaki, E. Stratakis, Mudasar Rashid, Merve Tutundzic, Y. Kuang, J. de Wild, Tom Aernouts, B. Vermang, M. Topič
Tandem solar cells present a promising solution to overcome the Schottky–Queisser efficiency limit of single-junction solar cells. In this article, an all–thin-film tandem solar cell based on perovskite (PK) top cell and chalcopyrite Cu (In, Ga) Se2 (CIGS) bottom cell is researched. Device optical simulations are validated on the top and bottom cells and employed for the analysis of PK/CIGS tandem cells. In particular, the optical effects of introduced laser reduced graphene oxide (rGO) layers at two positions in the tandem cell: 1) at the position between the top PK and bottom CIGS cell and 2) underneath the front transparent electrode. The purpose of introducing rGO layers is to improve the optoelectrical properties of the device, based on the tunable electronic and optical characteristics of rGO layers. Optical simulation results show that the parasitic absorption in rGO layers may noticeably affect the optical performance of the tandem cell if the layers are not optimized. The use of a thin and a few nanometer-thick rGO is suggested from the analysis if its parasitic absorption is not reduced. Directions for further optimization of optical rGO, including the reduction of parasitic absorption and tuning of the real part of the refractive index, are performed.
串联太阳能电池为克服单结太阳能电池的肖特基-奎瑟效率限制提供了一个有希望的解决方案。本文研究了一种基于钙钛矿(PK)顶电池和黄铜矿Cu (In, Ga) Se2 (CIGS)底电池的全薄膜串联太阳能电池。在顶部和底部电池上验证了器件光学模拟,并将其用于PK/CIGS串联电池的分析。特别是,引入激光的光学效应减少了串联电池中两个位置的氧化石墨烯(rGO)层:1)在顶部PK和底部CIGS电池之间的位置,2)在前部透明电极下方。引入rGO层的目的是基于rGO层的可调谐电子和光学特性来改善器件的光电性能。光学模拟结果表明,如果不优化氧化石墨烯层,氧化石墨烯层中的寄生吸收会显著影响串联电池的光学性能。分析表明,如果不降低氧化石墨烯的寄生吸收,建议使用薄的和几纳米厚的氧化石墨烯。进一步优化的方向,包括减少寄生吸收和调整实部折射率。
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引用次数: 0
In-Vivo Imaging of Ocular Microvasculature Using Swept-Source Optical Coherence Tomography Angiography in Seven Types of Lab Animals 用扫描源光学相干断层扫描对七种实验动物的眼部微血管系统进行体内成像
Pub Date : 2022-04-26 DOI: 10.3389/fphot.2022.867594
Xuan Wu, Jacqueline Chua, C. Ho, X. Yao, A. R. Muralidharan, R. Najjar, G. Tan, E. Tamm, L. Schmetterer, V. Barathi, Bingyao Tan
The purpose of this study is to characterize the retinal and choroidal vascular networks in some of the most common animal species using swept-source optical coherence tomography angiography (SS-OCTA). Retinal angiographic images were acquired from healthy, anesthetized animals of seven species (mouse, rat, pig, rabbit, guinea pig, chicken, and non-human primate). We generated the enface angiograms to visualize the different retinal vascular plexuses and the choroidal vascular plexus. Quantitative OCTA metrics, including perfusion density, vessel density, and fractal dimension, were compared amongst the different species. There was a noticeable difference in the OCTA enface maps of the distinct vascular layers amongst the various species. Specifically, the non-human primate retina has the highest level of perfusion density and vessel density, whereas the rabbit retina exhibited the lowest level of vessel density. The mouse and the rat retina shared similar vascular patterns, and there was no difference in the OCTA metrics. Using one specific SS-OCTA system for all experiments eliminated multiple instrument-dependent factors, but the lateral resolution was still affected by eye size. Effects of varying lateral resolution on vascular metrics were investigated via a simulation. Overall, we achieved a large field of view of the distinct retinal and choroidal vascular plexuses and quantified the vascular metrics in multiple species, which could serve as protocol guidance and atlas to study the retinal and choroidal vascular abnormalities and their roles in ocular diseases resembling in-vivo histology.
本研究的目的是利用扫描源光学相干断层扫描血管造影(SS-OCTA)来表征一些最常见动物物种的视网膜和脉络膜血管网络。视网膜血管造影图像来自7种健康的麻醉动物(小鼠、大鼠、猪、兔、豚鼠、鸡和非人灵长类动物)。我们制作了表面血管造影来显示不同的视网膜血管丛和脉络膜血管丛。定量OCTA指标,包括灌注密度、血管密度和分形维数,在不同物种之间进行比较。不同种属间不同维管层的OCTA面图存在显著差异。其中,非人灵长类动物视网膜的灌注密度和血管密度最高,而兔视网膜的血管密度最低。小鼠和大鼠的视网膜有相似的血管模式,在OCTA指标上没有差异。在所有实验中使用一个特定的SS-OCTA系统消除了多个仪器相关因素,但横向分辨率仍然受到眼睛大小的影响。通过模拟研究了不同的横向分辨率对血管指标的影响。总的来说,我们获得了不同的视网膜和脉络膜血管丛的大视野,并量化了多种物种的血管指标,这可以作为研究视网膜和脉络膜血管异常及其在类似体内组织学的眼部疾病中的作用的方案指导和图谱。
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引用次数: 0
Transverse Traveling-Wave and Standing-Wave Ray-Wave Geometric Beams 横向行波和驻波射线波几何光束
Pub Date : 2022-04-25 DOI: 10.3389/fphot.2022.855214
Zhaoyang Wang, Ruilin Long, Zhensong Wan, Zijian Shi, Xinjie Liu, Qiang Liu, X. Fu
Ray-wave geometric beam is an exotic kind of structured light with ray-wave duality and coupled diverse degrees of freedom (DoFs), which has attracted intense attention due to its potential applications in theories and applications. This work offers a new insight that the traditional ray-wave geometric beams can be seen as the transverse standing-wave (SW) beams, and can be decomposed into the superposition of transverse traveling-wave (TW) beams. We construct a generalized model for transverse TW and SW ray-wave geometric beams in the wave picture. In experiment, we exploit a digital hologram system with more flexible tunable DoFs to generate the transverse TW and SW beams, inspiring the exploration for the spatial wave structure of more complex structured light.
射线波几何光束是一种奇异的具有射线波对偶性和耦合多自由度的结构光,由于其在理论和应用中的潜在应用而备受关注。这项工作提供了一个新的见解,即传统的射线波几何光束可以被视为横向驻波(SW)光束,并可以分解为横向行波(TW)光束的叠加。我们构造了波浪图像中横向TW和SW射线波几何光束的广义模型。在实验中,我们开发了一种具有更灵活的可调谐DoF的数字全息图系统来产生横向TW和SW光束,这激发了对更复杂结构光的空间波结构的探索。
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引用次数: 1
A Survey for 3D Flame Chemiluminescence Tomography: Theory, Algorithms, and Applications (Invited) 三维火焰化学发光层析成像:理论、算法和应用(特邀)
Pub Date : 2022-03-29 DOI: 10.3389/fphot.2022.845971
Ying Jin, G. Situ
Combustion diagnostics play an essential role in energy engineering, transportation, and aerospace industries, which has great potential in combustion efficiency improvement and polluting emission control. The three-dimensional (3D) visualization of the combustion field and the measurement of key physical parameters such as temperature, species concentration, and velocity during the combustion process are important topics in the field of combustion diagnostics. Benefiting from the non-contact and non-intrusive advantages of the optical detection method as well as the advantages of the 3D full-field measurement of the measured field by computational tomography, flame chemiluminescence tomography (FCT) has the ability to realize non-intrusive and instantaneous 3D quantitative measurement and 3D full-field visualization of key physical parameters in the combustion process, which has crucial research significance in combustion diagnostics. In this study, we review the progress of FCT technique. First, we provide an extensive review of practical applications of FCT in state-of-the-art combustion diagnostics and research. Then, the basic concepts and mathematical theory of FCT are elaborated. Finally, we introduce the conventional reconstruction algorithm and proceed to more popular artificial intelligence-based algorithms.
燃烧诊断在能源工程、交通运输、航空航天等领域发挥着重要作用,在提高燃烧效率和控制污染排放方面具有巨大潜力。燃烧过程中燃烧场的三维可视化以及燃烧过程中温度、物质浓度和速度等关键物理参数的测量是燃烧诊断领域的重要课题。火焰化学发光层析成像(FCT)利用光学检测方法的非接触式、非侵入式的优点,以及计算层析成像对被测场进行三维全场测量的优点,能够实现燃烧过程中关键物理参数的非侵入式、瞬时三维定量测量和三维全场可视化,在燃烧诊断中具有至关重要的研究意义。本文就FCT技术的研究进展作一综述。首先,我们对FCT在最先进的燃烧诊断和研究中的实际应用进行了广泛的回顾。然后,阐述了FCT的基本概念和数学理论。最后,我们介绍了传统的重建算法,并进一步介绍了更流行的基于人工智能的算法。
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引用次数: 0
Development of a Fringe Printer With 0.35 μm Pixel Pitch 0.35 μm像素间距条纹打印机的研制
Pub Date : 2022-03-28 DOI: 10.3389/fphot.2022.843860
Takeshi Yamaguchi, H. Yoshikawa
The development of computational devices can provide computer-generated holograms (CGH) of over 100 Giga-pixel easily. The performance improvement of the graphical processing unit makes calculations faster with a normal personal computer. In contrast, the output device of CGH is not reported much. Since CGH is a fringe pattern, high resolution and fine pixel pitch are required for the output device. We have been developing a direct fringe printer, which consists of a laser, an X-Y stage, an SLM, and optical parts available on the market. Our previous report presented a liquid crystal panel, which was used for SLM that had full HD resolution and 7 μm pixel pitch. Since the Pixel pitch of the liquid crystal panel is not small enough for CGH, the optical setup works to demagnify the pixel pitch of the printed fringe pattern. To record high resolution CGH, the calculated fringe pattern is split and exposed in tiles by the X–Y stage. The results showed a 0.44 μm pixel pitch and over 100 Gpixel CGHs. However, to output more high-quality CGH, the development of pixel pitch and resolution is very important. In this paper, we review the optical system of a fringe printer that achieved an output of 0.35 μm pixel pitch CGH. We also investigate the performance of the new fringe printer.
计算设备的发展可以很容易地提供超过100千兆像素的计算机生成全息图。图形处理单元的性能改进使普通个人计算机的计算速度更快。相比之下,CGH的输出装置的报道并不多。由于CGH是条纹图案,所以输出设备需要高分辨率和精细的像素间距。我们一直在开发一种直接条纹打印机,该打印机由激光器、X-Y台、SLM和市场上可用的光学部件组成。我们之前的报告介绍了一种用于SLM的液晶面板,该面板具有全高清分辨率和7μm像素间距。由于液晶面板的像素间距对于CGH来说不够小,因此光学设置可以缩小打印条纹图案的像素间距。为了记录高分辨率CGH,计算出的条纹图案被X–Y台分割并曝光在瓷砖中。结果显示,像素间距为0.44μm,CGH超过100 Gpixel。然而,要输出更多高质量的CGH,像素间距和分辨率的发展是非常重要的。在本文中,我们回顾了实现0.35μm像素间距CGH输出的条纹打印机的光学系统。我们还研究了新型条纹打印机的性能。
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引用次数: 0
Deep-Learning Computational Holography: A Review (Invited) 深度学习计算全息:综述(特邀)
Pub Date : 2022-03-28 DOI: 10.3389/fphot.2022.854391
T. Shimobaba, David Blinder, Tobias Birnbaum, I. Hoshi, Harutaka Shiomi, P. Schelkens, T. Ito
Deep learning has been developing rapidly, and many holographic applications have been investigated using deep learning. They have shown that deep learning can outperform previous physically-based calculations using lightwave simulation and signal processing. This review focuses on computational holography, including computer-generated holograms, holographic displays, and digital holography, using deep learning. We also discuss our personal views on the promise, limitations and future potential of deep learning in computational holography.
深度学习发展迅速,利用深度学习研究了许多全息应用。他们已经表明,使用光波模拟和信号处理,深度学习可以胜过以前基于物理的计算。本文综述了使用深度学习的计算全息术,包括计算机生成的全息图、全息显示器和数字全息术。我们还讨论了我们对计算全息中深度学习的前景、局限性和未来潜力的个人看法。
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引用次数: 22
A Multi-Wavelength Phase Retrieval With Multi-Strategy for Lensfree On-Chip Holography 基于多策略的无透镜片上全息多波长相位恢复
Pub Date : 2022-03-28 DOI: 10.3389/fphot.2022.865666
Qinhua Wang, Jianshe Ma, Ping Su
Due to convenient operation and robust imaging, multi-wavelength phase retrieval has been widely applied in lensfree on-chip digital holographic microscope (LFOCDHM). Nevertheless, the insufficient diffraction variation and small number of measurements on the LFOCDHM make it difficult to eliminate the twin image by multi-wavelength phase retrieval. We propose a multi-wavelength phase retrieval for LFOCDHM based on energy constraint, global update strategy, and vector extrapolation acceleration. Simulations and experiments on the LFOCDHM show that our proposed method realizes efficient elimination effect and robust reconstruction with three wavelengths for illumination while maintaining fast convergence. More importantly, the proposed method is simple and non-parametric. It is believed that the proposed method could provide a promising solution for LFOCDHM.
由于操作方便、成像稳健,多波长相位恢复在无透镜片上数字全息显微镜(LFOCDHM)中得到了广泛的应用。然而,LFOCDHM上衍射变化不足和测量次数少,使得通过多波长相位检索来消除孪晶图像变得困难。我们提出了一种基于能量约束、全局更新策略和矢量外推加速的LFOCDHM多波长相位检索方法。在LFOCDHM上的仿真和实验表明,我们提出的方法在保持快速收敛的同时,实现了有效的消除效果和三波长照明的鲁棒重建。更重要的是,所提出的方法是简单和非参数的。相信所提出的方法可以为LFOCDHM提供一个很有前途的解决方案。
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引用次数: 1
Frontiers in Photonics Spot Light 光子学前沿聚光灯
Pub Date : 2022-03-09 DOI: 10.3389/fphot.2022.853456
Bahman Anvari, C. de Angelis, Shujuan Huang, N. Litchinitser, T. Poon, G. Scalari, B. Richards, M. Peccianti
Recognizing remarkable achievements and innovations is an important mission in scientific dissemination. The editors of Frontiers in Photonics would like to acknowledge and give visibility to some of the best recent research outputs and to their authors in this spot-light review.
认识到显著的成就和创新是科学传播的一项重要使命。《光子学前沿》的编辑们希望在这篇聚光灯评论中承认并介绍一些最新的研究成果及其作者。
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引用次数: 0
Compressive Interferenceless Coded Aperture Correlation Holography With High Imaging Quality (Invited) 高成像质量压缩无干涉编码孔径相关全息术(受邀)
Pub Date : 2022-03-04 DOI: 10.3389/fphot.2022.846731
Chao Liu, Tianlong Man, Yuhong Wan
Interferenceless coded aperture correlation holography (I-COACH) provides an alternative way for the 3D imaging of spatial incoherent illuminated or fluorescent sample. However, the low imaging signal-to-noise ratio (SNR) is one of the bottlenecks that restrict the application of I-COACH. The limitation is mainly originated from the strong bias level that presents in the recorded holograms. Phase shifting methods were implemented in I-COACH to eliminate the background noise while the multiple-exposures recording mechanism significantly reduces the temporal resolution of the system. In this paper, we proposed a compressive I-COACH imaging method with high reconstruction quality and without the sacrifice of the imaging speed. The 3D holographic image reconstruction was implemented under compressive sensing framework while only one single-exposure object hologram and one point spread hologram are necessary. High quality reconstructions were obtained using the proposed method, even for the down-sampled holograms. The imaging SNR of the I-COACH system was improved by a factor of more than 16.5% when comparing with the imaging SNR obtained by the conventional cross-correlation reconstruction method. The proposed method provides a fast and high-fidelity imaging method that can potentially benefit the imaging through scattering medium, partial aperture imaging, and other fields.
无干涉编码孔径相关全息术(I-COACH)为空间非相干照明或荧光样品的三维成像提供了一种替代方法。然而,低成像信噪比是制约I-COACH应用的瓶颈之一。这种限制主要源于记录的全息图中存在的强偏置水平。在I-COACH中实现了相移方法以消除背景噪声,而多次曝光记录机制显著降低了系统的时间分辨率。在本文中,我们提出了一种具有高重建质量且不牺牲成像速度的压缩I-COACH成像方法。三维全息图像重建是在压缩传感框架下实现的,而只需要一个单曝光物体全息图和一个点扩展全息图。使用所提出的方法获得了高质量的重建,即使对于下采样全息图也是如此。I-COACH系统的成像信噪比与传统的互相关重建方法相比提高了16.5%以上。所提出的方法提供了一种快速高保真的成像方法,可以潜在地有利于通过散射介质、部分孔径成像和其他领域进行成像。
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引用次数: 1
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Frontiers in photonics
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