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Optimization of Chaetoceros gracilis Microalgae Production for Fish Feeding Using an Airlift Photobioreactor 气升式光生物反应器生产微藻的优化研究
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5876
Oscar R. Pupo, Samira Garcia, L. Maré, S. Gómez, A. Bula
An experimental procedure was carried out to maximize Chaetoceros gracilis growth. Chaetoceros gracilis , marine microalgae, is considered for feeding fisheries with no GMO (Genetically Modified Organisms) to avoid human health hazards. Furthermore, following United Nations Resolution on water, the microalgae is grown in photobioreactors due to its low water usage. To maximize the microalgae growth, an experimental design was carried out to analyze the effects of Light Intensity, CO 2 supply per day, Sparger type, Photoperiod and Inlet airflow, pH and water temperature were monitored but not controlled. It was found that Light intensity and CO 2 supply per day have statistical significance. Out of three possible scenarios, 1700 lux and 80 gr/day of CO 2 , leads to a cell density at day three of 310×10 4 cel/mL which represents 20% more of the density attained in day two under bag (standard) growing conditions. It was also found that water Ph has also a strong effect over cell density.
为了使薄壁毛藻的生长最大化,进行了一种实验程序。为避免对人类健康造成危害,考虑将微藻毛羽(Chaetoceros gracilis)用于不含转基因生物的渔业。此外,根据联合国关于水的决议,由于微藻的用水量低,因此在光生物反应器中生长。为了最大限度地促进微藻生长,进行了试验设计,分析了光照强度、CO 2日供给量、Sparger类型、光周期和进水气流对微藻生长的影响,并对pH和水温进行了监测但不进行控制。光照强度和日均CO 2供给量差异有统计学意义。在三种可能的情况下,1700勒克斯和80克/天的二氧化碳,导致第三天的细胞密度为310×10 4细胞/mL,比在袋(标准)生长条件下第二天达到的密度多20%。研究还发现,水的Ph值对细胞密度也有很大的影响。
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引用次数: 0
Impedanciometric Study of Conducting Polymer Composite Films from Cassava Starch/ Poly (3,4-Ethylenedioxythiophene) 木薯淀粉/聚(3,4-乙烯二氧噻吩)导电聚合物复合膜的阻抗学研究
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5869
A. Alvaro, J. Reinaldo, S. Manuel
The aim of this study was to study, by electrochemical impedance spectroscopy, the electrochemical properties of a new conductive polymer composite made from cassava starch and poly (3, 4ethylenedioxythiophene) (PEDOT). The results show that the polymer composite shows excellent conductivity with resistance values in the order of kΩ and capacitance values in the order of mF as a result of possible interactions of polymer chains of cassava starch and PEDOT. The results allow to establish a high potentiality of the starch/PEDOT polymer composite for applications as batteries, charge accumulators or electrochemical actuators.
采用电化学阻抗谱法研究了由木薯淀粉和聚(3,4乙烯二氧噻吩)(PEDOT)制备的导电聚合物复合材料的电化学性能。结果表明,该聚合物复合材料具有优异的导电性能,其电阻值为kΩ,电容值为mF,这可能是木薯淀粉聚合物链与PEDOT相互作用的结果。研究结果表明,淀粉/PEDOT聚合物复合材料具有很高的应用潜力,可用于电池、电荷蓄电池或电化学致动器。
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引用次数: 0
Shelf Life of a Mixture of Pumpkin Puree (Cucurbita moschata) During Storage at 40xB0C 南瓜泥(Cucurbita moschata)混合物在40xbc保存期间的保质期
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5888
Norleyn M. Navas Guzman, L. Orellana, L. Quiñones
Pumpkin is used commonly as an ingredient for many food products because of its nutritional and health benefits. Current studies have been reported about pumpkin puree, but there is no information related to the mixture of flours and other ingredients. The development of a new formulation introduces into the market a different way of commercialization and consumption of pumpkin, favoring its production. The aim of this investigation was to determine the shelf life of the mixture by analyzing the physical-chemical and microbiological parameters of the product using a factorial experimental design of two factors, batches and time, with 3 and 13 levels respectively. The three levels of batches had 26 trays each one and processed with pumpkin puree, wheat flour, sugar, pasteurized egg, cinnamon, salt and butter. Potassium sorbate was added as a preservative agent, gum as a stabilizer and ascorbic acid as an acidulant agent. The mixture was packaged in polypropylene-polyethylene trays and stored at 4°C during 13 weeks. Two samples were taken weekly of each batch for physicochemical and microbial population analysis. The maximum charge of microorganisms reached was 4.07 Log CFU/g for aerobic mesophilic bacteria, 2.92 Log CFU/g for yeasts after day 28 and 1.48 Log CFU/g for molds after day 49. Coliforms were not found. It was observed a decrease in pH, water activity and redox potential. The results demonstrated that the shelf life of the product could be guaranteed up to day 35 when stored at 4°C.
南瓜通常被用作许多食品的原料,因为它的营养和健康益处。目前有关于南瓜泥的研究报道,但没有关于面粉和其他成分混合物的信息。新配方的开发为市场引入了一种不同的南瓜商业化和消费方式,有利于南瓜的生产。采用3个、13个水平的因子试验设计,通过分析产品的理化参数和微生物学参数,确定产品的保质期。三个级别的批次每一个有26个托盘,用南瓜泥、小麦粉、糖、巴氏消毒鸡蛋、肉桂、盐和黄油加工。加入山梨酸钾作为防腐剂,口香糖作为稳定剂,抗坏血酸作为酸化剂。将混合物包装在聚丙烯-聚乙烯托盘中,4°C保存13周。每批每周取两份样品进行理化和微生物种群分析。好氧中温细菌的最大微生物负荷为4.07 Log CFU/g, 28 d后酵母达到2.92 Log CFU/g, 49 d后霉菌达到1.48 Log CFU/g。没有发现大肠菌群。观察到pH值、水活度和氧化还原电位下降。结果表明,在4°C的条件下,该产品的保质期可达35天。
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引用次数: 2
Kinetics of Color Change in Squash (Cucurbita maxima) During Frying by Immersion at Atmospheric Pressure and Vacuum Pressure 常压和真空浸泡油炸过程中南瓜(Cucurbita maxima)颜色变化动力学
Pub Date : 2018-07-10 DOI: 10.19026/ajfst.14.5893
Jhonatan Rodriguez, Armando Alvis, Mario Perez
The goal of this study was to determine the kinetics of the change of color in squash (Cucurbita maxima) during frying by immersion under conditions of atmospheric pressure and vacuum. Methodologically, the color of the rind of the external surface of the product conditioning in geometry of cylinders circulars, was evaluated in terms of the parameters CIELAB concerning L*, a* and b* post-process of frying under conditions of temperature of 150, 170 and 190°C, pressure of 40 and 80 kPa and during the time of processing time of 30 to 270 sec in intervals of 60 sec. The darkening of the surface of the product by effect of the process of frying in both procedures was evident in the decrease of the parameter L* as well as the parameters a* and b*, being more severe effect of darkening of the frying atmospheric higher temperature and experiencing changes of zero order for L*, zero for a* and first order for b*. The unit of the temperature and the speed of the reaction is explained by the Arrhenius equation. The activation energy obtained for the parameters of L*, a* and b* were 58.050, 13.709 and 53.545 kJ/mol and 32.082, 13.336 and 63.768 kJ/mol for the process of frying to the vacuum and frying in atmospheric conditions respectively.
本研究的目的是确定在常压和真空条件下浸泡油炸过程中南瓜(Cucurbita maxima)颜色变化的动力学。在方法上,根据CIELAB在150、170和190℃条件下油炸后处理的L*、a*和b*参数,对圆柱体圆形形状的产品外表面外皮的颜色进行了评价。40和80 kPa,期间的压力处理时间30 - 270秒的间隔60秒。产品的表面变暗的煎的过程的影响在这两个过程是明显的减少参数L * a * b以及参数*,更严重影响煎黯淡的大气温度较高和经历变化的零阶L * 0 b * *和一阶。温度和反应速度的单位可以用阿伦尼乌斯方程来解释。L*、a*和b*的活化能分别为58.050、13.709和53.545 kJ/mol,真空油炸和常压油炸的活化能分别为32.082、13.336和63.768 kJ/mol。
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引用次数: 0
Effects of Enzymatic Hydrolysis on the Physicochemical and Structural Properties of Cassava Bagasse (Manihot esculenta Cranz). 酶解对甘蔗渣理化和结构性质的影响。
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5889
M. V. Ortega, D. Luján-Rhenals, Jairo G. Salcedo
This study evaluated the effect of enzymatic hydrolysis on the physicochemical and structural properties of cassava bagasse ( Manihot esculenta Cranz ). Cassava bagasse is a byproduct of the cassava starch process with limited applications in the industry. Were applied enzymatic treatments to three ratios a substrate (cassava bagasse): Buffer volume (1:10, 1:15, 1:20). Were used two commercial enzymes, cellulase (CelluSEB TL) and α-amylase (Licquozyme supra 2.2X). The physicochemical and structural analysis was performed after each treatment, including a control. The physicochemical analysis showed that the cassava bagasse had a high content of carbohydrates (61,19%) and fiber (22,63%); additionally, there were significant differences (p -1 corresponding to the cellulose chemical bond CH, as well as the decrease in the intensity of the band 2927 cm-1 corresponding to the CH bonds and CH2, which may be related to a decrease of the crystallinity in the enzymatically treated bagasse. It was found that, due to its physicochemical composition, cassava bagasse is a material that could be used for biotechnology or food purposes; moreover, enzymatic hydrolysis produces the decrystallization of cellulose and significant changes in its physicochemical properties.
研究了酶解对甘蔗渣(Manihot esculenta Cranz)理化性质和结构特性的影响。木薯甘蔗渣是木薯淀粉加工的副产品,在工业上的应用有限。对底物(木薯甘蔗渣)进行三种比例的酶处理:缓冲体积(1:10,1:15,1:20)。采用纤维素酶(CelluSEB TL)和α-淀粉酶(Licquozyme supra 2.2X)两种商品化酶。每次处理后进行理化和结构分析,包括对照。理化分析表明,木薯甘蔗渣具有较高的碳水化合物(61,19%)和纤维(22,63%)含量;此外,纤维素化学键CH与CH2之间存在显著差异(p -1), CH2与CH键对应的2927 cm-1波段强度降低,这可能与酶处理后甘蔗渣结晶度降低有关。研究发现,由于其物理化学成分,木薯甘蔗渣是一种可用于生物技术或食品用途的材料;此外,酶水解产生纤维素的脱晶和其物理化学性质的显著变化。
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引用次数: 1
Mathematical Modelling of the Rehydration Kinetics of Yam (Dioscorea rotundata) Chips in a Microwave Oven 山药薄片在微波炉中再水化动力学的数学建模
Pub Date : 2018-07-10 DOI: 10.19026/ajfst.14.5899
Angelica Torregroza, C. A. Garcia, F. Romero
Aim of study was to model rehydration of yam chips dried in a microwave oven at different power levels. We used a completely randomized32 factorial design corresponding to the numeric variables of power (420, 560 and 700 W) and mass (50, 60 and 70 g) in 1 cm thick×3 cm-diameter yam chips. 3 repetitions were made for a total of 27 experimental units. 2, 5 g yam chips were rehydrated using a 2.5 g/100 mL NaCl solution at 25°C. Power and mass variables showed significant differences (p 97,9% and 0,001
研究了在不同功率下微波干燥的山药片的再水化过程。我们使用了一个完全随机的32因子设计,对应于1厘米thick×3厘米直径的薯片的功率(420、560和700 W)和质量(50、60和70 g)的数值变量。3次重复,共27个实验单位。2.5 g山药片用2.5 g/100 mL NaCl溶液在25℃下再水化。功率和质量变量差异显著(p < 97,9%, 0.001
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引用次数: 0
Evaluation of Spray Drying Conditions in the Survival of Bifidobacterium bifidum in a Product Based on Whey and Mango Pulp of the Variety Magdalena River (Mangifera indica L.) 喷雾干燥条件对马格达莱纳河(Mangifera indica L.)芒果浆乳清制品中两歧双歧杆菌存活的影响
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5870
Fernando A. Mendoza-Corvis, Margarita Arteaga Márquez, Omar Andrés Pérez Sierra
Food incorporated with probiotics provides essential nutrients to the consumers and at the same time has a positive effect on the human health. This study aimed to determine the optimal conditions for the spray drying process for achieving the maximum survival of Bifidobacterium bifidum ATCC 11863 in a beverage based on whey and mango pulp, using maltodextrin as an encapsulating agent. A central composite design with 22 experiments was used, in which different variables. Such as concentrations of maltodextrin (15-35%), inlet temperatures (120-160°C), outlet temperatures (65-77°C) and rotor speed (20000-28000 rpm) were tested to obtain the optimal conditions for the intended new food product. Also, physicochemical variables of the developed food product were determined, such as water activity, humidity, the angle of repose, particle size and yield of the final product. The results showed that B. bifidum was affected by the concentration of maltodextrin. The B. bifidum survival ranged from of 59.16%; the product was incorporated with 20% maltodextrin, to 76.36% probiotic survival when 25% maltodextrin more used for the food product development. In this regard, the treatment 13 presented a microbiological count of 2.3×10 11 CFU/g, is this the highest survival percentage of B. bifidum .
添加了益生菌的食品在为消费者提供必需营养素的同时,对人体健康也有积极的影响。本研究旨在确定以麦芽糊精为包封剂,以乳清和芒果浆为基础的饮料中两歧双歧杆菌ATCC 11863最大存活率的喷雾干燥工艺的最佳条件。采用22个试验的中心组合设计,其中不同的变量。通过对麦芽糊精浓度(15-35%)、进口温度(120-160°C)、出口温度(65-77°C)和转子转速(20000-28000 rpm)等进行测试,以获得拟生产新食品的最佳条件。测定了所研制食品的理化指标,如水分活度、湿度、休止角、粒度、最终产品得率等。结果表明,麦芽糖糊精浓度对两歧双歧杆菌的生长有一定影响。两歧双歧杆菌的存活率为59.16%;该产品掺入20%的麦芽糊精后,益生菌存活率达到76.36%,其中25%的麦芽糊精多用于食品开发。在这方面,处理13的微生物计数为2.3×10 11 CFU/g,这是两歧双歧杆菌存活率最高的处理。
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引用次数: 0
Sweet Chili Flour Obtainment (Capsicum annuum) and Physicochemical and Functional Properties Determination 甜辣椒粉的制备及其理化和功能特性的测定
Pub Date : 2018-07-10 DOI: 10.19026/ajfst.14.5867
Armando Alvis, A. Buelvas, G. Arrázola
The aim of the study was obtained Chili flour and their physicochemical and functional characterization. The chili powder or flour, for industrial and domestic use, facilitates its conservation and delays its deterioration, due to the reduction of its humidity. Capsicum species are fruit and vegetable products that have a good drying behaviour due to their resistance to color changes caused by temperature gradients and preserving the product quality (sensorial and nutritional characteristics). The drying kinetics of chili ( Capsicum annuum ) was established to obtain flour with drying trays and its physiochemical and functional properties were determined. The samples presented 9±0.2° Brix, 91.18% humidity and green coloration were adapted to 0, 1×2, 3×2, 3 cm samples, dried at 50, 60 and 70°C temperatures on a tray with forced air speed of 2,7m/s, continual weight. When comparing the physiochemical variables %H, aw, pH and color, a significant difference was observed for the study treatments between them (p<0.05) which did not show up on the acidity property. Regarding the functional properties, between the evaluated treatments, the WRC (water retention capacity) and the FBC (fat binding capacity), there were significant differences (p<0.05) while on EC (emulsifier capacity) values were more even. Physicochemical and functional properties of chili can be preserved by controlled drying.
本研究的目的是获得辣椒粉及其理化特性和功能特性。工业和家庭使用的辣椒粉或面粉,由于其湿度的降低,有利于其保存和延缓其变质。辣椒品种是具有良好干燥性能的水果和蔬菜产品,因为它们抵抗温度梯度引起的颜色变化并保持产品质量(感官和营养特性)。采用干燥盘法制备辣椒粉,建立了辣椒粉的干燥动力学,测定了辣椒粉的理化性质和功能特性。样品的白利度为9±0.2°,湿度为91.18%,颜色为绿色,分别适用于0,1×2, 3×2, 3 cm的样品,在50,60和70°C的温度下在托盘上干燥,强制风速为2,7m/s,连续称重。在比较理化指标%H、aw、pH和颜色时,各处理间差异显著(p<0.05),但在酸度方面差异不显著。在功能特性方面,各处理间WRC(保水能力)和FBC(脂肪结合能力)差异显著(p<0.05),而EC(乳化剂能力)差异较为均匀。控制干燥可以保持辣椒的理化特性和功能特性。
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引用次数: 1
Drying Uniformity Analysis in a Tray Dryer: An Experimental and Simulation Approach 托盘式干燥机干燥均匀性分析:实验与模拟方法
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5901
S. Natalia, Caicedo Mauricio, A. Hugo, F. Hugo
This study aimed to use a Computational Fluid Dynamics (CFD) simulation of a tray dryer to investigate the possible causes of non-uniformity in the absolute final product humidity after the drying process in an empirically constructed tray dryer. In the experimental part, turmeric, which is the rhizome of a perennial herbaceous plant with interesting properties for the cosmetic and food industries, was dried. Pieces of turmeric with identical dimensions were placed in different zones of the dryer and their initial and final humidity values were empirically measured. In the simulations, the momentum and energy equations were solved with ANSYS software (Student v.17.2). The K-epsilon model was used to model the turbulence and the boundary and process conditions were identical to those used in the experiment. Although the steady-state CFD simulations show a homogenous local temperature distribution in the dryer, the local turbulence intensity profiles display dead zones with low turbulence values. Furthermore, these dead zones are consistent with areas where the product exhibits higher humidity. Low local turbulence values can negatively affect the local mass transfer coefficient, which may explain the experimental results of this study.
本研究旨在利用计算流体动力学(CFD)模拟托盘干燥机,探讨在经验构建的托盘干燥机中干燥过程中最终产品绝对湿度不均匀的可能原因。在实验部分,姜黄是一种多年生草本植物的根茎,对化妆品和食品工业具有有趣的特性,被干燥。将尺寸相同的姜黄块放置在干燥机的不同区域,并对其初始和最终湿度值进行经验测量。在仿真中,利用ANSYS软件(Student v.17.2)求解动量方程和能量方程。采用K-epsilon模型来模拟湍流,边界和过程条件与实验中使用的相同。尽管稳态CFD模拟显示干燥机内部局部温度分布均匀,但局部湍流强度曲线显示出低湍流值的死区。此外,这些死区与产品显示较高湿度的区域一致。低的局部湍流值会对局部传质系数产生负面影响,这可以解释本研究的实验结果。
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引用次数: 6
Mechanical and Viscoelastic Properties of Mozzarella Cheese 马苏里拉奶酪的力学和粘弹性特性
Pub Date : 2018-07-10 DOI: 10.19026/AJFST.14.5881
C. A. Garcia, Armando Alvis, G. Arrázola
The aim of this study is to determine the mechanical properties and the viscoelastic characteristics of the mozzarella cheese made from buffalo milk. Mozzarella cheese has a characteristic structure as the curd undergoes a stretching process in hot water that leads to the formation of a fibrous structure. This affects the number, strength and type of bond between the protein molecules that form the basis for the rheological properties exhibited. The mechanical properties and the viscoelastic characteristics of buffalo mozzarella were measured in the present study. Uniaxial compression tests between parallel plates with an extra 5.8 cm compression were carried out in cheese cubes of 2 cm edge in order to estimate Peleg model parameters such as the compressive stress, deformation modulus, degree of elasticity and A and B constants. Cheese cubes were compressed and decompressed at a deformation rate of 20 cm/min up to 40% of their height through a complete compression-decompression cycle. For a compression area of 4.0×10 -4 m 2 a maximum compressive strength of 28.0±3 N, a compressive stress of 7.0×10 4 Pa and a deformation modulus of E D = 175.0 kPa were obtained. The degree of elasticity was 73.0±8.5%, which indicates that mozzarella cheese has a very elastic texture. The values for A and B constants of the Peleg model were as follows: A = 0.83 and B = 0.11. Buffalo mozzarella exhibitselastic recovery levels suitable for technological use, as well as viscoelastic solid properties.
本研究的目的是确定由水牛奶制成的马苏里拉奶酪的机械性能和粘弹性特性。马苏里拉奶酪具有一种特殊的结构,因为凝块在热水中经历了拉伸过程,导致纤维结构的形成。这影响了蛋白质分子之间的键的数量、强度和类型,这些键形成了所展示的流变性能的基础。对水牛马苏里拉干酪的力学性能和粘弹性特性进行了测试。为了估计Peleg模型的压应力、变形模量、弹性度和A、B常数等参数,在边缘为2 cm的奶酪立方体中进行了平行板间的单轴压缩试验,外加5.8 cm的压缩量。通过一个完整的压缩-减压循环,以20cm /min的变形速率压缩和解压奶酪立方体至其高度的40%。压缩面积为4.0×10 -4 m2时,最大抗压强度为28.0±3 N,压应力为7.0×10 4 Pa,变形模量为ed = 175.0 kPa。弹性度为73.0±8.5%,表明马苏里拉奶酪具有很好的弹性质地。Peleg模型的A、B常数分别为:A = 0.83, B = 0.11。水牛马苏里拉奶酪具有适合技术使用的弹性恢复水平,以及粘弹性固体特性。
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引用次数: 0
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Advance Journal of Food Science and Technology
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