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Contribution of the Microbiome as a Tool for Estimating Wine’s Fermentation Output and Authentication 微生物组作为估算葡萄酒发酵产量和鉴定的工具的贡献
Pub Date : 2019-07-01 DOI: 10.5772/INTECHOPEN.85692
D. Anagnostopoulos, E. Kamilari, D. Tsaltas
Wine is the alcoholic beverage which is the product of alcoholic fermentation, usually, of fresh grape must. Grape microbiome is the source of a vastly diverse pool of filamentous fungi, yeast, and bacteria, the combination of which plays a crucial role for the quality of the final product of any grape must fermentation. In recent times, the significance of this pool of microorganisms has been acknowledged by several studies analyzing the microbial ecology of grape berries of different geographical origins, cultural practices, grape varieties, and climatic conditions. Furthermore, the microbial evolution of must during fermentation process has been overstudied. The combination of the microbial evolution along with metabolic and sensorial characterizations of the produced wines could lead to the suggestion of the microbial terroir. These aspects are today leading to open a new horizon for products such as wines, especially in the case of PDO-PGI products. The aims of this review is to describe (a) how the microbiome communities are dynamically differentiated during the process of fermentation from grape to ready-to-drink wine, in order to finalize each wine’s unique sensorial characteristics, and (b) whether the microbiome could be used as a fingerprinting tool for geographical indication, based on high-throughput sequencing (HTS) technologies. Nowadays, it has been strongly indicated that microbiome analysis of grapes and fermenting musts using next-generation sequencing (NGS) could open a new horizon for wine, in the case of protected designation of origin (PDO) and protected geographical indication (PGI) determination.
葡萄酒是一种含酒精的饮料,通常用新鲜的葡萄汁发酵而成。葡萄微生物群是丝状真菌、酵母菌和细菌的来源,它们的组合对任何葡萄发酵的最终产品的质量起着至关重要的作用。近年来,通过对不同地理来源、文化实践、葡萄品种和气候条件的葡萄果实的微生物生态学分析,这一微生物池的重要性得到了承认。此外,对酵母发酵过程中微生物的进化也进行了深入的研究。微生物的进化以及所生产的葡萄酒的代谢和感官特征的结合可以导致微生物风土的建议。今天,这些方面为葡萄酒等产品,特别是PDO-PGI产品开辟了新的视野。这篇综述的目的是描述(a)在从葡萄到可饮用葡萄酒的发酵过程中,微生物群落是如何动态分化的,以最终确定每种葡萄酒独特的感官特征,以及(b)微生物群落是否可以作为基于高通量测序(HTS)技术的地理标志指纹工具。近年来,在受保护的原产地名称(PDO)和受保护的地理标志(PGI)测定的情况下,利用新一代测序(NGS)对葡萄和发酵剂进行微生物组分析可以为葡萄酒开辟新的领域。
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引用次数: 9
Effects of Vine Water Status on Yield Components, Vegetative Response and Must and Wine Composition 葡萄水分状况对产量组成、营养反应和酒、酒成分的影响
Pub Date : 2019-06-25 DOI: 10.5772/INTECHOPEN.87042
P. Baeza, P. Junquera, Emilio Peiro, J. Lissarrague, D. Uriarte, M. Vilanova
Despite Vitis vinifera L. is a drought-tolerant species—rainfed traditionally grown in a very diversity of climates—irrigation has more and more become a usual practice aimed to obtain regular yields along seasons and to control must composition. Results on vineyard irrigation are dependent on the timing, length and intensity of the water deficit. From budbreak to flowering, shoot growth is very sensitive to water stress, while reproductive growth is almost unaffected. Severe water deficit during fruit set can reduce yield by affecting ovary cell multiplication and expansion. During maturation water stress induces yield reduction by limiting berry growth; along this phase must composition is also affected. There is a positive, linear relationship between must sugar content and available water; however, no relationship has been found to either total acidity or pH. Biosynthesis of anthocyanins and fruity aromas is enhanced by water deficit. Usually, wines from moderate irrigation treatments scored the highest. There is a general agreement that severe, long water deficits diminish must quality, leaf area, fertility and yield, and it has a negative carryover effect on the next seasons by limiting wood reserves to be used the following seasons.
尽管葡萄是一种耐旱品种——传统上是在多种气候条件下生长的雨养品种——灌溉越来越成为一种常规做法,目的是在不同季节获得稳定的产量,并控制其成分。葡萄园灌溉的效果取决于水分亏缺的时间、长度和强度。从萌芽到开花,芽部生长对水分胁迫非常敏感,而生殖生长几乎不受影响。坐果期严重缺水会影响子房细胞的增殖和膨胀,从而降低产量。成熟期水分胁迫通过限制浆果生长导致产量降低;沿着这一阶段,必须的成分也受到影响。必须糖含量与有效水分呈线性正相关;然而,没有发现总酸度或ph的关系。花青素的生物合成和水果香气是由水分不足增强。通常,适度灌溉的葡萄酒得分最高。人们普遍认为,长期严重缺水会降低质量、叶面积、肥力和产量,并通过限制下一季节使用的木材储备,对下一季节产生负面的延续效应。
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引用次数: 14
Production and Marketing of Low-Alcohol Wine 低度酒的生产与销售
Pub Date : 2019-06-21 DOI: 10.5772/INTECHOPEN.87025
T. Bucher, K. Deroover, C. Stockley
Moderate wine consumption may be associated with specific health benefits and a healthy lifestyle. However, increased amounts of ethanol are cytotoxic and associated with adverse health outcomes. Alcohol reduction in wine might be an avenue to reduce alcohol related harm without forcing consumers to compromise on lifestyle and benefit from positive aspects of moderate consumption. The aim of this review is to give an overview of viticultural and pre and post fermentation methods to produce low-alcohol wine, and to summarize the current evidence on the consumer acceptance and behaviour related to low-alcohol wine. Strategies for the labelling and marketing of wines with reduced alcohol content are discussed.
适度饮用葡萄酒可能与特定的健康益处和健康的生活方式有关。然而,乙醇含量的增加具有细胞毒性,并与不良健康结果相关。减少葡萄酒中的酒精含量可能是一种减少酒精相关危害的途径,同时又不会迫使消费者在生活方式上妥协,并从适度消费的积极方面获益。本文综述了低度葡萄酒的栽培方法、前发酵方法和后发酵方法,并总结了目前消费者对低度葡萄酒的接受程度和行为。讨论了降低酒精含量的葡萄酒的标签和营销策略。
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引用次数: 7
Strategies to Improve the Freshness in Wines from Warm Areas 提高温暖地区葡萄酒新鲜度的策略
Pub Date : 2019-06-08 DOI: 10.5772/INTECHOPEN.86893
A. Morata, I. Loira, J. Fresno, C. Escott, M. Bañuelos, W. Tesfaye, C. González, F. Palomero, J. A. Lepe
Trends in wine consumption are continuously changing. The latest in style is fresh wine with moderate alcohol content, high acidity, and primary aromas reminiscent of grapes, whereas certain fermentative volatiles may also influence the freshness of the wine. In addition, the effects of climate change on the composition of the grapes (high sugar content and low acidity) are adverse for the quality of the wine, also considering the microbiological stability. Herein, different strategies aiming at improving wine freshness are presented, and their performance in winemaking is discussed: among them, the addition of organic acids able to inhibit malolactic fermentation such as fumaric acid; the use of acidifying yeasts for alcoholic fermentation, such as Lachancea thermotolerans ; and the selection of non- Saccharomyces yeasts with β -glucosidase activity in order to release terpene glycosides present in the must.
葡萄酒消费的趋势在不断变化。最新的风格是新鲜的葡萄酒,酒精含量适中,酸度高,初级香气让人想起葡萄,而某些发酵挥发物也可能影响葡萄酒的新鲜度。此外,气候变化对葡萄成分的影响(高糖含量和低酸度)对葡萄酒的品质不利,也考虑到微生物稳定性。本文提出了提高葡萄酒新鲜度的不同策略,并讨论了它们在酿酒中的性能:其中,添加富马酸等能够抑制苹果乳酸发酵的有机酸;使用酸化酵母进行酒精发酵,如耐高温酵母;选择具有β -葡萄糖苷酶活性的非酵母菌,以释放葡萄柚中存在的萜烯苷。
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引用次数: 9
Somatic Variation and Cultivar Innovation in Grapevine 葡萄的体细胞变异与品种创新
Pub Date : 2019-05-30 DOI: 10.5772/INTECHOPEN.86443
P. Carbonell-Bejerano, C. Royo, N. Mauri, J. Ibáñez, José Miguel Martínez Zapater
Paradoxically, continuous vegetative multiplication of traditional grapevine cultivars aimed to maintain cultivar attributes in this highly heterozygous species ends in the accumulation of considerable somatic variation. This variation has long contributed to cultivar adaptation and evolution under changing environmental and cultivation conditions and has also been a source of novel traits. Understanding how this somatic variation originates provides tools for genetics-assisted tracking of selected variants and breeding. Potentially, the identification of the mutations causing the observed phenotypic variation can now help to direct genome editing approaches to improve the genotype of elite traditional cultivars. Molecular characterization of somatic variants can also generate basic information helping to understand gene biological function. In this chapter, we review the state of the art on somatic variation in grapevine at phenotypic and genome sequence levels, present possible strategies for the study of this variation, and describe a few examples in which the genetic and molecular basis or very relevant grapevine traits were successfully identified.
矛盾的是,在这个高度杂合的品种中,传统葡萄品种为了保持品种属性而进行的连续无性繁殖,最终导致了相当大的体细胞变异的积累。长期以来,这种变异有助于品种在不断变化的环境和栽培条件下的适应和进化,也是新性状的来源。了解这种体细胞变异的起源为遗传学辅助跟踪选择的变异和育种提供了工具。潜在地,鉴定导致观察到的表型变异的突变现在可以帮助指导基因组编辑方法,以改善精英传统品种的基因型。体细胞变异的分子特征也可以产生有助于理解基因生物学功能的基本信息。在本章中,我们回顾了葡萄在表型和基因组序列水平上的体细胞变异的最新进展,提出了研究这种变异的可能策略,并描述了一些成功鉴定葡萄性状的遗传和分子基础或非常相关的例子。
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引用次数: 17
Alcohol Reduction by Physical Methods 用物理方法减少酒精
Pub Date : 2019-05-29 DOI: 10.5772/INTECHOPEN.85989
M. Schmitt, M. Christmann
Alcohol reduction of wine has gained significance worldwide. There are several technologies available to reduce the alcohol content in a targeted way. This chapter explains the principles of alcohol reduction by physical methods. Different membrane processes such as osmotic distillation and the two-step dealcoholization process of reverse osmosis combined with osmotic distillation are compared with distillation processes such as vacuum rectification and spinning cone column. An alternative approach the membrane coupling of ultraand nanofiltration is described as well. All those technologies appear more or less suitable to reduce the alcohol content in a targeted way. Nevertheless improper handling can cause severe quality losses for the wine. Therefore, enologists should have a thorough understanding of the technologies to avoid negative impact on wine quality through the treatment.
葡萄酒的酒精减量在世界范围内具有重要意义。有几种技术可以有针对性地降低酒精含量。本章解释了用物理方法减少酒精的原理。将渗透精馏、反渗透结合渗透精馏两步脱醇工艺等不同膜工艺与真空精馏、旋锥塔蒸馏工艺进行了比较。本文还介绍了一种替代方法——超滤和纳滤的膜耦合。所有这些技术似乎都或多或少适合有针对性地减少酒精含量。然而,不当的处理会导致葡萄酒严重的质量损失。因此,酿酒师应该对这些技术有深入的了解,以避免通过处理对葡萄酒品质产生负面影响。
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引用次数: 0
Yeast Strain Optimization for Enological Applications 酿酒应用的酵母菌株优化
Pub Date : 2019-05-27 DOI: 10.5772/INTECHOPEN.86515
D. Ferreira, J. Noble
In the world of winemaking, tradition and innovation have always been side by side, on the one hand a culture of several centuries and on the other the need to constantly improve and answer new challenges. Consumers’ preferences, climate changes, and fermentation efficiency are some of the modern questions that winemakers have to consider. Yeast, at the center of the fermentation, has revealed itself as the perfect platform to answer many of these challenges. By understanding the metabolism and the genetic basis that modulate specific phenotypes of yeast during fermentation, an era of yeast optimization has surfaced in the last decades and pushed research even further. In this chapter we will focus the atten-tion on two of the most successful techniques to that end, quantitative trait locus (QTL) and evolutionary engineering. QTL relies on a highly precise identification of the genome regions that control a phenotype of interest. The transfer of these regions to selected wine yeasts is then possible by a technique called backcrossing. Evolutionary engineering induces the yeast itself to modify its genetic background to adapt to a selective pressure and improve its fitness. The right choice of pressure leads to the improvement of its performances in enological conditions.
在葡萄酒酿造的世界里,传统和创新一直肩并肩,一方面是几个世纪的文化,另一方面是不断改进和应对新挑战的需要。消费者的喜好、气候变化和发酵效率是酿酒师必须考虑的一些现代问题。酵母,在发酵过程的中心,已经显示出自己是一个完美的平台来回答这些挑战。通过了解酵母在发酵过程中调节特定表型的代谢和遗传基础,酵母优化时代在过去几十年浮出水面,并推动研究进一步发展。在本章中,我们将把注意力集中在两个最成功的技术上,数量性状位点(QTL)和进化工程。QTL依赖于对控制感兴趣表型的基因组区域的高度精确鉴定。然后通过一种称为回交的技术将这些区域转移到选定的葡萄酒酵母上。进化工程诱导酵母自身修改其遗传背景以适应选择压力并提高其适应性。正确选择压力可以改善其在酿酒条件下的性能。
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引用次数: 1
Air Depleted and Solvent Impregnated Cork Powder as a New Natural and Sustainable Wine Fining Agent 无气溶剂浸渍软木塞粉作为一种新型天然可持续葡萄酒精剂
Pub Date : 2019-05-23 DOI: 10.5772/INTECHOPEN.85691
Luís Filipe Ribeiro, Márcia Martins, F. Nunes
We recently proposed a simple methodology to improve cork powder waste adsorption properties through vacuum degassing and solvent impregnation, to use this abundant and cheap material as a new wine fining agent. Its applicability was first shown for red wine 4-ethylphenol (4-EP) and 4-ethylguaiacol (4-EG) reduction. Nowadays, the presence of 4-EP and 4-EG is a serious problem in the wine industry, known as “ Brett character ” , by the negative aroma imparted by these volatile phenols (VPs) to red wine. There are only some curative treatments to remove these compounds without impacting negatively on wine quality. Optimised cork powder was used successfully as a new treatment for the reduction of these negative VPs (41 – 75% for 4-EP and 40 – 69% for 4-EG) increasing at the same time wine sensory performance. Wine treated with cork powder reduced 6.9% phenolic acids and catechin and 2.3% monomeric anthocyanins without any significant change in colour intensity. In this chapter, the cork complex structure is discussed, besides the impact of its use in wine containing VPs on physicochemical composition and quality. This new application of this natural, abundant and cheap material has the potential of being a new wine fining agent with low environmental impact.
我们最近提出了一种简单的方法,通过真空脱气和溶剂浸渍来改善软木塞粉末对废物的吸附性能,将这种丰富而廉价的材料作为一种新的葡萄酒精制剂。该方法首次应用于红酒4-乙基苯酚(4-EP)和4-乙基愈创木酚(4-EG)的还原。如今,4-EP和4-EG的存在是葡萄酒行业的一个严重问题,被称为“布雷特特性”,因为这些挥发性酚(VPs)给红酒带来了负面的香气。只有一些治疗方法可以去除这些化合物,而不会对葡萄酒质量产生负面影响。优化后的软木粉作为一种新的处理方法,成功地降低了这些负vp (4-EP为41 - 75%,4-EG为40 - 69%),同时提高了葡萄酒的感官性能。用软木塞粉处理过的葡萄酒,其酚酸和儿茶素的含量降低了6.9%,单体花青素的含量降低了2.3%,但颜色强度没有明显变化。在本章中,讨论了软木塞的复合结构,以及在含vp的葡萄酒中使用软木塞对其理化成分和品质的影响。这种天然、丰富、廉价的材料的新应用有可能成为一种低环境影响的新型葡萄酒精制剂。
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引用次数: 2
Yeast from Distillery Plants: A New Approach 从酿酒厂提取酵母:一种新方法
Pub Date : 2019-05-16 DOI: 10.5772/INTECHOPEN.86291
Beatriz García-Béjar, P. Fernández-Pacheco, A. Briones, M. Arévalo-Villena
Nowadays, there is more and more interest in the microbiological resources from different ecosystems, not only because this would allow knowing more about the microbial biodiversity related with these substrata but also because it provides an opportunity to study their characteristics and technological properties which may be of potential interest. This knowledge may allow finding future biotechnological applications for these microorganisms on bio-conservation and reuse of agricultural by-products and may also lead to studies on the improvement of raw material processing. Some raw materials and processing plants in wine and related industries constitute a suitable place for yeast growth; for example, musts, wines in cellars, piquettes, bagasse, pomace, grape skins and yeast lees in the ethanol industry all provide an inexhaustible supply of yeasts. Few microbiological studies have been published so far about the biodiversity of the yeast population in distillery plants. For that reason, the aim of this research was to determine yeast biodiversity and their distribution in different distillery plants in the La Mancha region which are at least 100 years old.
目前,人们对来自不同生态系统的微生物资源越来越感兴趣,不仅因为这可以让人们更多地了解与这些基质相关的微生物多样性,而且因为它提供了一个研究它们的特征和技术特性的机会,这些特性和技术特性可能是潜在的兴趣。这一知识可能有助于发现这些微生物在生物保护和农业副产品再利用方面的未来生物技术应用,也可能导致对原材料加工改进的研究。葡萄酒及相关行业的一些原料和加工厂构成了酵母生长的适宜场所;例如,在乙醇工业中,酒窖里的酒、葡萄皮、甘蔗渣、果渣、葡萄皮和酵母渣都能提供取之不尽的酵母。迄今为止,关于酿酒厂酵母种群的生物多样性的微生物学研究还很少发表。因此,本研究的目的是确定酵母的生物多样性及其在拉曼查地区至少有100年历史的不同酿酒厂中的分布。
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引用次数: 1
The Microvine: A Versatile Plant Model to Boost Grapevine Studies in Physiology and Genetics 微藤:一种促进葡萄生理学和遗传学研究的多功能植物模型
Pub Date : 2019-05-10 DOI: 10.5772/INTECHOPEN.86166
A. Pellegrino, C. Romieu, Markus Rienth, L. Torregrosa
The microvine is a grapevine somatic variant. The Vvgai1 mutation results in a miniaturization of the vegetative organs of the plant keeping fruit size intact and a systematic conversion of tendrils into inflorescences. The physiological characterization of the vegetative and reproductive development of the microvine makes it possible to infer kinetic data from spatial phenotypes. This biological model allows experiments on vine and grape development in tightly controlled conditions, which greatly accelerate physiology, molecular biology, as well as genetic studies. After introducing the main biological properties of the microvine, main results from various research programs performed with the microvine model will be presented.
微藤是葡萄藤的体细胞变种。Vvgai1突变导致植物营养器官的小型化,保持果实大小完整,卷须系统地转化为花序。微藤营养发育和生殖发育的生理特征使得从空间表型推断动力学数据成为可能。这种生物学模型允许在严格控制的条件下对葡萄藤和葡萄的发育进行实验,这大大加快了生理学、分子生物学和遗传学的研究。在介绍了微藤的主要生物学特性之后,将介绍用微藤模型进行的各种研究项目的主要结果。
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引用次数: 6
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Advances in Grape and Wine Biotechnology
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