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The impact of COVID-19 and anti-immigration policy on an undocumented student in the United States: a qualitative case study COVID-19和反移民政策对美国无证学生的影响:定性案例研究
Pub Date : 2023-09-14 DOI: 10.29392/001c.75168
Chulwoo Park, Shannon Shimada, Patricia Angelica
Background The change of Deferred Action for Childhood Arrivals (DACA) program has significantly impacted DACA students’ mental health in the United States. The study aimed to conduct a qualitative case study for understanding the effects of DACA program on one undocumented college student’s life during the COVID-19 pandemic. Methods We conducted a two-component qualitative case study, the online survey and the subsequent in-depth interview, with a DACA college student, Leah (pseudonym), in California who was in an ongoing fear of deportation before and during the COVID-19 pandemic. We used Questionnaire-9 (PHQ-9) and General Anxiety Disorder-7 (GAD-7) in the online survey to measure her level of depression and anxiety. QSR NVivo was used to analyze the verbatim transcription. Results Leah’s anxiety and depression level just before the Supreme Court’s decision to the DACA termination in June 2020 was higher than during the COVID-19 pandemic in 2021. Leah’s concern still remained due to the uncertain legal status in the United States even after the presidential election. Conclusions During 2020-2022, the anti-immigration policy, the COVID-19 pandemic, and the presidential election has impacted a DACA student’s mental health status. However, because of the unstable legal status and fear of deportation in the United States, our participant’s anxiety and depression level maintained high. From our participant’s standpoint, more policy support is needed to sustain her legal status, financial stability, and mental health during the pandemic.
童年来美暂缓遣返(DACA)计划的改变对美国DACA学生的心理健康产生了重大影响。该研究旨在进行定性案例研究,以了解DACA计划在COVID-19大流行期间对一名无证大学生生活的影响。方法我们对一名DACA大学生Leah(化名)进行了双成分定性案例研究、在线调查和随后的深度访谈,她在2019冠状病毒病大流行之前和期间一直担心被驱逐出境。我们在在线调查中使用问卷-9 (PHQ-9)和一般焦虑障碍-7 (GAD-7)来测量她的抑郁和焦虑水平。采用QSR NVivo进行逐字转录分析。结果在2020年6月最高法院决定终止DACA之前,莉亚的焦虑和抑郁水平高于2021年新冠肺炎大流行期间。即使在总统大选之后,由于美国法律地位的不确定性,Leah的担忧仍然存在。2020-2022年期间,反移民政策、COVID-19大流行和总统大选对DACA学生的心理健康状况产生了影响。然而,由于在美国的法律地位不稳定和害怕被驱逐出境,我们的参与者的焦虑和抑郁水平保持在很高的水平。从我们的参与者的角度来看,在大流行期间,需要更多的政策支持来维持她的法律地位、财务稳定和心理健康。
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引用次数: 0
Time to rethink medical disinfection from a planetary health perspective 是时候从全球健康的角度重新思考医疗消毒了
Pub Date : 2023-09-14 DOI: 10.29392/001c.87862
Kate M Homyer, Felicity V Mehendale
Disinfection products can have substantial environmental impacts which include associated manufacturing emissions, toxicity to marine life and potential adverse health outcomes. Despite this, disinfection is under-represented in sustainability approaches. Disinfection is a key part of healthcare provision and as such should be reflected in healthcare sustainability strategies. The adverse environmental effects of several common disinfectants are highlighted here. Sustainable alternatives should be considered. Hypochlorous acid (HOCl) is a potential alternative disinfectant that could be used in sustainability strategies, carrying a minimal toxicity profile compared to hypochlorite (bleach) and the unique ability to be made on site. Better clarity is needed regarding the environmental impact of disinfectants used in the healthcare setting and healthcare providers should move to seek sustainable alternatives such as hypochlorous acid.
消毒产品可能对环境产生重大影响,包括相关的制造排放、对海洋生物的毒性和潜在的不利健康后果。尽管如此,消毒在可持续性方法中的代表性不足。消毒是卫生保健提供的关键部分,因此应反映在卫生保健可持续性战略中。这里着重介绍几种常见消毒剂对环境的不利影响。应该考虑可持续的替代方案。次氯酸(HOCl)是一种潜在的可替代消毒剂,可用于可持续发展战略,与次氯酸盐(漂白剂)相比,其毒性最小,并且具有现场制造的独特能力。需要更清楚地了解卫生保健环境中使用的消毒剂对环境的影响,卫生保健提供者应寻求可持续的替代品,如次氯酸。
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引用次数: 0
Willingness to pay (WTP) for HIV and AIDS services in Africa: a descriptive thematic systematic review 非洲艾滋病毒和艾滋病服务的支付意愿:一项描述性专题系统综述
Pub Date : 2023-08-29 DOI: 10.29392/001c.85122
J. Byabagambi, M. Limmer, B. Hollingsworth
The African continent has the highest burden of HIV and AIDS, with its response to HIV and AIDS largely donor supported. However, in the face of declining donor support, alternative ways to sustainably support HIV and AIDS responses in Africa are paramount. This systematic review explores the willingness to pay (WTP) for HIV services in Africa as a potentially more sustainable HIV and AIDS service cost recovery approach. A comprehensive systematic search for literature was conducted in PubMed, EMBASE, Web of Science, and CINAHL and websites of HIV and AIDS organisations for studies published until 30 June 2023. Studies were included if they were about WTP, HIV and AIDS services, were conducted in Africa and were published in English. Studies were excluded if they used methods other than WTP and were not about an HIV service. JBI critical appraisal tools were used to assess for quality and risk of bias. Information on the HIV service, the study methods, and factors influencing WTP were extracted. A descriptive thematic analysis was undertaken to synthesise evidence. The findings are summarised in tables and graphs. 5,141 records were identified and screened for eligibility from the initial search. After title and abstract screening and removing duplicates, twenty-three articles from 10 countries with 20,780 study participants were included in the final review. There is an uneven distribution of WTP studies across different types of HIV services and across countries. There is evidence of a willingness to pay for HIV services, with the proportion of people reported in individual studies that are willing to pay ranging from 34.3% to 97.1%. However, in most studies (77.3%, 17/22), the amount people are willing to pay cannot cover the full-service cost in an open market. Factors associated with WTP include socio-economic status, beliefs, and knowledge about HIV services. This systematic review presents evidence of cost recovery from HIV programs. The main finding is that other resources, beyond out-of-pocket payments, are needed to meet the total cost of any service. This has implications for providing (non-donor-funded) services on a sustainable level in the long term. In interpreting the findings of this study, limitations such as excluding papers not published in English need to be considered. Registration: PROSPERO, CRD42021275215.
非洲大陆的艾滋病毒和艾滋病负担最重,其应对艾滋病毒和艾滋病的措施在很大程度上得到了捐助者的支持。然而,面对捐助者不断减少的支持,以其他方式可持续地支持非洲的艾滋病毒和艾滋病防治工作至关重要。这项系统审查探讨了非洲艾滋病毒服务的支付意愿,作为一种潜在的更可持续的艾滋病毒和艾滋病服务成本回收方法。在PubMed、EMBASE、Web of Science和CINAHL以及HIV和艾滋病组织的网站上对截至2023年6月30日发表的研究进行了全面系统的文献检索。如果研究是关于WTP、艾滋病毒和艾滋病服务、在非洲进行并以英语发表的,则包括在内。如果研究使用了WTP以外的方法,并且与HIV服务无关,则将其排除在外。JBI关键评估工具用于评估偏差的质量和风险。提取了有关HIV服务的信息、研究方法和影响WTP的因素。进行了描述性专题分析以综合证据。调查结果汇总在表格和图表中。从最初的搜索中确定了5141条记录,并对其进行了资格筛选。经过标题和摘要筛选并删除重复,来自10个国家的23篇文章和20780名研究参与者被纳入最终审查。WTP研究在不同类型的艾滋病毒服务和各国之间的分布不均衡。有证据表明,人们愿意为艾滋病毒服务付费,个别研究中报告的愿意付费的人数比例从34.3%到97.1%不等。然而,在大多数研究中(77.3%,17/22),人们愿意支付的金额无法支付公开市场的全方位服务费用。与WTP相关的因素包括社会经济地位、信仰和对HIV服务的了解。这篇系统综述提供了从艾滋病项目中收回成本的证据。主要发现是,除了自付费用外,还需要其他资源来支付任何服务的总成本。这对长期提供可持续水平的(非捐助者资助的)服务具有影响。在解释这项研究的结果时,需要考虑一些局限性,例如排除非英文发表的论文。注册:PROSPERO,CRD42021275215。
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引用次数: 0
Self-care interventions for advancing sexual and reproductive health and rights – implementation considerations 促进性健康和生殖健康及权利的自我护理干预措施——实施考虑因素
Pub Date : 2023-08-21 DOI: 10.29392/001c.84086
Manjulaa Narasimhan, C. Logie, J. Hargreaves, W. Janssens, Mandip Aujla, P. Steyn, Erica van der Sijpt, A. Hardon
Self-care refers to the ability of people to promote their own health, prevent disease, maintain health, and cope with illness and disability, with or without the support of a health or care worker. Self-care interventions are tools that support self-care as additional options to facility-based care. Recognizing laypersons as active agents in their own health care, the World Health Organization (WHO)’s global normative guideline on self-care interventions recommends people-centred, holistic approaches to health and well-being for sexual and reproductive health and rights. Examples of such interventions include pregnancy self-testing, self-monitoring of blood glucose and/or blood pressure during pregnancy and self-administration of injectable contraception. Building on previous studies and aligning with the WHO classification for self-care, we discuss nine key implementation considerations: agency, information, availability, utilization, social support, accessibility, acceptability, affordability, and quality. The implementation considerations form the foundation of a model implementation framework that was developed using an ecological health systems approach to support sustainable changes in health care delivery.
自我保健是指人们在有或没有保健或护理人员的支持的情况下促进自身健康、预防疾病、保持健康以及应对疾病和残疾的能力。自我保健干预措施是支持自我保健的工具,作为基于设施的护理的额外选择。世界卫生组织(世卫组织)关于自我保健干预措施的全球规范准则承认非专业人员是其自身保健的积极推动者,因此建议采取以人为本的全面办法,促进性健康和生殖健康及权利的健康和福祉。这类干预措施的例子包括妊娠自我检测、妊娠期间自我监测血糖和/或血压以及自我注射避孕药具。在以往研究的基础上,并与世卫组织自我保健分类保持一致,我们讨论了九个关键的实施考虑因素:机构、信息、可得性、利用、社会支持、可及性、可接受性、可负担性和质量。实施方面的考虑构成了使用生态卫生系统方法开发的模型实施框架的基础,以支持卫生保健服务的可持续变化。
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引用次数: 0
Why should we invest in health? Evidence from the lens of second-order benefits of health 我们为什么要投资于健康?健康二阶效益的证据
Pub Date : 2023-08-18 DOI: 10.29392/001c.85124
D. Francis, Neha Dumka, A. Kotwal, Dutta Pk
Life expectancy and access to health care have both increased globally in recent decades, and these innovations have significantly improved well-being. As a result, health reforms are increasingly becoming a top concern in all countries in order to address/increase accessibility, affordability, and availability of quality services, as well as improve spending efficiencies. There are many evidenced benefits which are secondary to the health benefits of those programmes. However, second-order benefits of health schemes have received little attention and are rarely discussed in mainstream policy and practice. The paper intends to contribute to broader initiatives to recognise health schemes as vital in achieving equitable and inclusive development that benefits the entire community. The logical framework technique was used to analyse the health schemes’ second-order benefits. The primary and secondary objectives, methods, outcomes, and impacts were compared. Apart from the health programmes’ own objectives, the analysis revealed extensive benefits such as employment opportunities, local partnerships with communities, decreased inequalities and population-level impacts on socio-economic development, women empowerment, increase in literacy and school completion rates, and decrease in out-of-pocket expenditure. The economic impact of investing in health systems by creating high-quality jobs and purchasing goods and services from local businesses is highlighted by the second-order health advantages, which are in relation to the primary benefits. Not only do healthcare systems play an important role in the economies of the nation, but also in the social lives of these communities.
近几十年来,全球的预期寿命和获得医疗保健的机会都有所增加,这些创新显著改善了福祉。因此,为了解决/提高优质服务的可及性、可负担性和可获得性,以及提高支出效率,卫生改革正日益成为所有国家的首要关注点。有许多已证明的益处是次要于这些方案的健康益处的。然而,医疗计划的二阶效益很少受到关注,主流政策和实践中也很少讨论。该文件旨在为更广泛的举措做出贡献,以认识到卫生计划对实现惠及整个社区的公平和包容性发展至关重要。使用逻辑框架技术来分析健康计划的二阶效益。比较了主要和次要目标、方法、结果和影响。除了卫生方案本身的目标外,分析还揭示了广泛的好处,如就业机会、与社区的地方伙伴关系、减少不平等和人口水平对社会经济发展的影响、赋予妇女权力、提高识字率和完成学业率,以及减少自付支出。通过创造高质量的就业机会和从当地企业购买商品和服务来投资卫生系统的经济影响突出表现在与主要利益相关的二阶卫生优势上。医疗保健系统不仅在国家经济中发挥着重要作用,而且在这些社区的社会生活中也发挥着重要的作用。
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引用次数: 0
Access to medicines in the Indian Public Health System – what works and what does not? A review of the National Health Mission Common Review Mission Reports (2007-2021) 在印度公共卫生系统中获得药品——什么有效,什么无效?国家卫生特派团共同审查特派团报告(2007-2021)
Pub Date : 2023-08-18 DOI: 10.29392/001c.84486
E. Hannah, Nisha Basheer, Neha Dumka, A. Kotwal
Since the inception of India’s flagship “National Health Mission” (formerly known as the National Rural Health Mission), considerable efforts have been made to ensure access to quality and affordable healthcare, including medicines. Over time, there has been a differential, yet noticeable improvement in the availability and access to medicines across states. This underscores the need to review and analyse various state-specific practices in India supported or recommended by the NHM, to identify key enablers and barriers. Consequently, a review of NHM’s Common Review Mission (CRM) reports between 2007 and 2021 was undertaken. All findings relevant to medicines were identified, retrieved and analysed. Core themes pertaining to medicines included: mechanisms adopted by states for procurement and supply chain management, health system factors influencing the supply chain, the convergence of national health programs, the rollout of the Free Drugs Service Initiative and Comprehensive Primary Health Care, and awareness among the healthcare providers and community. The article further expounds on these themes in the Indian context, highlighting the determinants of access to medicines as well as their interlinkages. In conclusion, it underscores the need to strengthen the overall health system to accelerate universal access to free essential medicines at public health facilities.
自印度旗舰“国家卫生使命”(前身为国家农村卫生使命)成立以来,已经做出了相当大的努力,以确保获得高质量和负担得起的医疗保健,包括药品。随着时间的推移,各州在药品的供应和获取方面有了不同但明显的改善。这突出表明,有必要审查和分析国家卫生管理局支持或建议的印度各州的各种具体做法,以确定关键的促成因素和障碍。因此,对2007年至2021年间NHM的共同审查团(CRM)报告进行了审查。对所有与药物有关的发现进行了鉴定、检索和分析。与药品有关的核心主题包括:各州采用的采购和供应链管理机制、影响供应链的卫生系统因素、国家卫生计划的衔接、免费药品服务倡议和全面初级卫生保健的推出,以及医疗保健提供者和社区的认识。文章进一步阐述了印度背景下的这些主题,强调了获得药物的决定因素及其相互联系。最后,它强调需要加强整个卫生系统,以加快在公共卫生设施普及免费基本药物。
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引用次数: 0
The effect of the anglophone crisis on youth sexual and reproductive health in the Northwest region of Cameroon: a qualitative study 英语危机对喀麦隆西北地区青年性健康和生殖健康的影响:一项定性研究
Pub Date : 2023-08-16 DOI: 10.29392/001c.84239
J. Fonkwo, Micheal Besong, Justin Ngong, Alexander Glazier, Drusilla A. Talawa, A. Alio
The ongoing armed conflict in the two English-speaking regions of Cameroon, named the “Anglophone crisis”, has had a deleterious impact on the health and wellbeing of the populations inhabiting these regions, especially adolescents and youth, but there is sparse evidence on the effect of the crisis on adolescent and youth sexual and reproductive health (SRH). This study aimed to describe the effects of the crisis on adolescent SRH and identify stakeholder recommendations for strategies to address the SRH of youth in their crisis-impacted communities. This was a secondary analysis of qualitative data collected during discussion forums with 316 community stakeholders, including parents (n=63), youths (n=81), religious leaders (29), neighborhood leaders (n=23), secondary school teachers (n=28), health professionals (n=37), pharmacists (n=5), local drug vendors (n=13), media representatives (n=12), and non-governmental organization representatives (n=29), in Bamenda, northwest region, Cameroon. A total of 12 homogenous discussion groups of 5 to 41 participants in each were conducted between May and July 2020. Session recordings were transcribed and then analyzed using Dedoose qualitative data management software and a text analysis approach. The results indicate that the crisis led to internal displacements and idleness among adolescent and youth, which increased their social and economic vulnerabilities, including to sexual violence and transactional sex. Resulting SRH effects were unplanned pregnancies, sexually transmitted Infections, unsafe abortions, maternal mortality, and psychosocial distress. Community stakeholders recommended that strategies to improve adolescent and youth SRH outcomes in these conflict-hit regions focus on spreading information about SRH, providing SRH services, and addressing issues of idleness. The armed conflict in the Northwest region of Cameroon has had consequences on adolescent and youth’s adverse SRH outcomes, such as unintended pregnancies, transactional sex, rapes, unsafe abortions, and maternal death. The findings of this study highlight the importance of raising awareness on SRH risks of adolescents and youth and the necessity of adopting a community approach in developing interventions and policies to improve adolescent and youth SRH in regions affected by armed conflicts.
喀麦隆两个讲英语的地区正在发生的武装冲突,被称为"英语国家危机",对居住在这些地区的居民,特别是青少年的健康和福祉产生了有害影响,但关于这场危机对青少年性健康和生殖健康的影响的证据很少。本研究旨在描述危机对青少年性健康和健康的影响,并确定利益相关者建议的策略,以解决受危机影响社区青少年性健康和健康问题。这是对在喀麦隆西北部巴门达地区与316个社区利益相关者进行讨论论坛期间收集的定性数据进行的二次分析,这些利益相关者包括家长(n=63)、青年(n=81)、宗教领袖(29)、社区领导人(n=23)、中学教师(n=28)、卫生专业人员(n=37)、药剂师(n=5)、当地药品供应商(n=13)、媒体代表(n=12)和非政府组织代表(n=29)。在2020年5月至7月期间,共进行了12个同质讨论小组,每个小组有5至41名参与者。将会话记录转录,然后使用Dedoose定性数据管理软件和文本分析方法进行分析。结果表明,危机导致青少年和青年内部流离失所和无所事事,这增加了他们的社会和经济脆弱性,包括性暴力和交易性行为。由此产生的性生殖健康影响包括意外怀孕、性传播感染、不安全堕胎、孕产妇死亡率和社会心理困扰。社区利益相关者建议,在这些受冲突影响的地区,改善青少年性健康和生殖健康结果的战略应侧重于传播有关性健康和生殖健康的信息,提供性健康和生殖健康服务,并解决懒惰问题。喀麦隆西北地区的武装冲突对青少年和青年的性健康和生殖健康产生了不利影响,如意外怀孕、交易性行为、强奸、不安全堕胎和孕产妇死亡。这项研究的结果强调了提高对青少年和青年性健康和生殖健康风险的认识的重要性,以及在制定干预措施和政策以改善受武装冲突影响地区青少年和青年性健康和生殖健康方面采取社区方法的必要性。
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引用次数: 1
HOCl vs OCl−: clarification on chlorine-based disinfectants used within clinical settings HOCl与OCl−:澄清临床环境中使用的氯基消毒剂
Pub Date : 2023-08-16 DOI: 10.29392/001c.84488
F. Mehendale, G. Clayton, Kate M Homyer, D. Reynolds
Disinfection is a mainstay of infection prevention, the importance of which was highlighted throughout the SARS-CoV-2 pandemic. There is frequent misuse of terminology surrounding chlorine solutions in the literature. This leads not only to confusion but has potentially dangerous outcomes, as inappropriate mixing of chlorine solutions with other disinfectants or cleaning solutions can lead to the release of chlorine gas. This article provides a resource for accurate terminology surrounding chlorine-based disinfection and clarifies some of the key inaccuracies, including the pH-dependent nature of chlorine species distribution of hypochlorous acid (HOCl) (neutral/acidic chlorine solution) and hypochlorite (OCl-) (alkaline chlorine solution). Misuse and misunderstanding of chlorine solutions and the terminology used can be harmful therefore this is an essential resource for those utilising chlorine as a disinfectant.
消毒是预防感染的支柱,在整个严重急性呼吸系统综合征冠状病毒2型大流行期间,消毒的重要性得到了强调。文献中关于氯溶液的术语经常被误用。这不仅会导致混乱,而且会产生潜在的危险后果,因为氯溶液与其他消毒剂或清洁溶液的不当混合可能会导致氯气的释放。本文提供了一个关于氯消毒的准确术语资源,并澄清了一些关键的不准确之处,包括次氯酸(HOCl)(中性/酸性氯溶液)和次氯酸(OCl-)(碱性氯溶液)的氯物种分布的pH依赖性。对氯溶液和所用术语的滥用和误解可能是有害的,因此这是那些将氯用作消毒剂的人的重要资源。
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引用次数: 0
Rapid response mechanism in conflict-affected settings of Cameroon: lessons learned from a multisector intervention for internally displaced persons 喀麦隆受冲突影响环境中的快速反应机制:从对国内流离失所者的多部门干预中吸取的经验教训
Pub Date : 2023-08-13 DOI: 10.29392/001c.85011
Lundi-Anne Omam, Alain Metuge
The Northwest and Southwest regions of Cameroon have experienced armed conflict over the last seven years, characterized by mass displacement and limited access to health care and social amenities. In response, an emergency intervention programme called “rapid response mechanism” (RRM) was initiated to provide lifesaving services to internally displaced persons. The intervention was multisectoral and included a health component, nutrition, water hygiene and sanitation, and child protection. RRM served communities of Ekondo Titi district, marked with high levels of insecurity, poor telecommunication networks and limited geographical access. Although the RRM was designed to provide rapid and lifesaving interventions to the affected populations; the RRM, in this case, was only initiated one year after the conflict escalated. Key benefits of the RRM included: (i) increased access to health care services through its integrated community case management approach, (ii) development of full displacement map within the health district, further strengthening the health system by establishing a community-based surveillance and response system through community health workers, and (iii) assisting the health district team in mass vaccination campaigns in seven of the nine health areas, which were otherwise completely inaccessible. The RRM model was largely primary health care focused compared to other RRMs in conflict-affected countries. It is important for RRM benefit packages to be harmonized to enable better preparedness and responses in conflicts. There is also a need for better coordination among sectoral partners to ensure improved response in crises.
喀麦隆西北和西南地区在过去七年中经历了武装冲突,其特点是大规模流离失所,获得医疗保健和社会福利的机会有限。作为回应,启动了一项名为“快速反应机制”的紧急干预方案,为国内流离失所者提供救生服务。干预措施是多部门的,包括健康组成部分、营养、水卫生和环境卫生以及儿童保护。RRM为Ekondo Titi区的社区提供服务,这些社区的特点是高度不安全、电信网络差和地理接入有限。尽管RRM旨在为受影响人群提供快速、挽救生命的干预措施;在这种情况下,RRM是在冲突升级一年后才启动的。RRM的主要好处包括:(i)通过其综合社区病例管理方法增加了获得医疗保健服务的机会,(ii)在卫生区内制定全面的流离失所地图,通过社区卫生工作者建立基于社区的监测和应对系统,进一步加强卫生系统,以及(iii)协助卫生区工作队在9个卫生区中的7个开展大规模疫苗接种活动,否则这些地区将完全无法进入。与受冲突影响国家的其他RRM相比,RRM模式主要侧重于初级卫生保健。重要的是,要统一RRM福利包,以便在冲突中更好地做好准备和作出反应。部门合作伙伴之间也需要更好地协调,以确保更好地应对危机。
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引用次数: 1
Utility of formalin-fixed, paraffin-embedded prostate biospecimens from low-resource settings for use in next-generation sequencing studies in African-descent populations 福尔马林固定、石蜡包埋的低资源前列腺生物样本在非洲裔人群下一代测序研究中的应用
Pub Date : 2023-08-10 DOI: 10.29392/001c.84541
E. Kaninjing, K. Adeniji, A. Gachii, P. Jibrin, J. Obafunwa, C. Ogo, Mohammed Faruk, A. Popoola, O. Fatiregun, O. Oluwole, W. Aiken, M. Jackson, Robin Roberts, Shravana Kumar Jyoti, C. Dial, M. Jalloh, L. Niang, M. Ndoye, Jason A White, Balasubramanyam Karanam, Damian Francis, Denise Gibbs, Kathryn R Brignole, C. Yates, C. Ragin, F. Odedina, Damali N Martin
Men of African ancestry experience higher burden from prostate cancer compared to men of other ancestral backgrounds. Limitations in the availability of high-quality biospecimens hinder the inclusion of this population in genetic studies of prostate cancer. The use of formalin-fixed paraffin-embedded (FFPE) tissues represent a potential rich source of genetic material particularly in some international settings, where fresh frozen tissue is difficult to obtain. In this study, we investigate the feasibility of using FFPE biospecimens acquired from various international sites for utility in next-generation sequencing. A total of 976 FFPE blocks were collected between 2002 and 2017 from six international sites in Africa and the Caribbean representing three consortia: Prostate Cancer Transatlantic Consortium; African-Caribbean Cancer Consortium; and Men of African Descent and Carcinoma of the Prostate. Genomic DNA was checked for quality and quantity. Differences in mean quality control (QC) for pre-and-post pathology training were assessed using t-test. Pearson chi-square with trend analysis examined association between time-category and QC success status. Association of continuous DNA quality (Q129/Q41 ratio) and time of specimen collection was estimated with linear regression. Samples with a DNA quantity >0.2µg and a Q129/Q41 ratio >0.00225 were submitted for whole exome sequencing (WES). There was a positive relative percentage change in DNA quantity from 2002 to 2017 for Jamaica, Kenya and Senegal. There was a decline in DNA quantity over the same time period for Nigeria. There was a statistically significant improvement in quality of samples from Kenya (P=0.032), Nigeria (P<.001) and Senegal (P=0.043). There was a significant improvement in the collected DNA sample quality over time with an R2 of 0.12. FFPE samples from low-resource settings could potentially provide sufficient DNA for WES. Improvements in biospecimen collection processing and storage for research are needed in some of these settings.
与其他祖先背景的男性相比,非洲裔男性患前列腺癌的负担更高。高质量生物标本可用性的限制阻碍了将这一人群纳入前列腺癌的遗传研究。使用福尔马林固定石蜡包埋(FFPE)组织代表了遗传物质的潜在丰富来源,特别是在一些难以获得新鲜冷冻组织的国际环境中。在这项研究中,我们探讨了利用来自不同国际站点的FFPE生物标本用于下一代测序的可行性。2002年至2017年期间,从非洲和加勒比地区的六个国际站点共收集了976个FFPE块,代表三个联盟:前列腺癌跨大西洋联盟;非洲-加勒比癌症协会;非裔男性与前列腺癌的关系。检查了基因组DNA的质量和数量。采用t检验评估病理培训前后的平均质量控制(QC)差异。皮尔逊卡方与趋势分析检验了时间类别与QC成功状态之间的关系。用线性回归估计连续DNA质量(Q129/Q41比值)与标本采集时间的关系。样品DNA量>0.2µg, Q129/Q41比值>0.00225提交全外显子组测序(WES)。从2002年到2017年,牙买加、肯尼亚和塞内加尔的DNA数量出现了正的相对百分比变化。在同一时期,尼日利亚的DNA数量有所下降。肯尼亚(P=0.032)、尼日利亚(P< 0.001)和塞内加尔(P=0.043)的样品质量有统计学意义的改善。随着时间的推移,收集的DNA样本质量有显著改善,R2为0.12。来自低资源环境的FFPE样本可能为WES提供足够的DNA。在其中一些环境中,需要改进用于研究的生物标本收集、处理和储存。
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Journal of global health reports
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