Pub Date : 2016-04-11DOI: 10.1186/s12952-016-0051-2
Julio F Marchini, Ayumi Aurea Miyakawa, Flavio Tarasoutchi, José Eduardo Krieger, Pedro Lemos, Kevin Croce
Background: Patients with severe aortic stenosis have increased levels of prothrombotic and proinflammatory microparticles (MP), and MPs actively regulate pathological processes that lead to atherothrombotic cardiovascular events. Shear stress is a validated stimulus of MP production, and abnormal shear stress in aortic stenosis increases MP release in ex-vivo studies. We hypothesized that in patients with severe aortic stenosis, percutaneous replacement of the aortic valve (TAVR) would reduce abnormal shear stress and would decrease levels of circulating MPs.
Findings: The experimental protocol utilized flow cytometry (FC) and nanoparticle tracking analysis (NTA) to quantify circulating plasma MP levels in aortic stenosis patients at baseline and 5 days after TAVR. The baseline and 5 day MP counts measured by FC were 6.10⋅10(5) ± 1.21⋅10(5) MP/μL and 5.74⋅10(5) ± 9.54⋅10(4) MP/μL, respectively (p = 0.91). The baseline and 5 day MP counts measured by NTA were 9.29⋅10(13) ± 1.66⋅10(13) MP/μL and 3.95⋅10(14) ± 3.11⋅10(14) MP/μL, respectively (p = 0.91). When MPs were stratified by cell source, there was no difference in pre/post TAVR endothelial, platelet, or leukocyte MP levels.
Conclusion: Levels of circulating MPs do not change acutely following TAVR therapy for aortic stenosis. Trial registered at clinicaltrials.gov NCT02193035 on July 11, 2014.
背景:严重主动脉瓣狭窄患者血栓形成前和促炎微粒(MP)水平升高，MPs积极调节导致动脉粥样硬化性血栓性心血管事件的病理过程。剪切应力是一种有效的MP生成刺激，在离体研究中，主动脉狭窄的异常剪切应力会增加MP的释放。我们假设在严重主动脉瓣狭窄的患者中，经皮主动脉瓣置换术(TAVR)可以减少异常剪切应力并降低循环MPs水平。研究结果:实验方案利用流式细胞术(FC)和纳米颗粒跟踪分析(NTA)来量化主动脉瓣狭窄患者在基线和TAVR后5天的循环血浆MP水平。FC测定的基线和5 d MP计数分别为6.10⋅10(5)±1.21⋅10(5)MP/μL和5.74⋅10(5)±9.54⋅10(4)MP/μL (p = 0.91)。NTA测定的基线和5 d MP计数分别为9.29⋅10(13)±1.66⋅10(13)MP/μL和3.95⋅10(14)±3.11⋅10(14)MP/μL (p = 0.91)。当MPs按细胞来源分层时，TAVR前后内皮细胞、血小板或白细胞的MP水平没有差异。结论:TAVR治疗主动脉瓣狭窄后，循环MPs水平无明显变化。试验于2014年7月11日在clinicaltrials.gov注册，编号NCT02193035。
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Pub Date : 2016-04-01DOI: 10.1186/s12952-016-0049-9
F. D’Abramo, U. Goerling, C. Guastadisegni
{"title":"Targeted drugs and Psycho-oncological intervention for breast cancer patients","authors":"F. D’Abramo, U. Goerling, C. Guastadisegni","doi":"10.1186/s12952-016-0049-9","DOIUrl":"https://doi.org/10.1186/s12952-016-0049-9","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0049-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-12DOI: 10.1186/s12952-016-0048-x
Kota Sato, K. Nishiguchi, K. Maruyama, Satoru Moritoh, Kosuke Fujita, Yoshikazu Ikuta, H. Kasai, T. Nakazawa
{"title":"Topical ocular dexamethasone decreases intraocular pressure and body weight in rats","authors":"Kota Sato, K. Nishiguchi, K. Maruyama, Satoru Moritoh, Kosuke Fujita, Yoshikazu Ikuta, H. Kasai, T. Nakazawa","doi":"10.1186/s12952-016-0048-x","DOIUrl":"https://doi.org/10.1186/s12952-016-0048-x","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0048-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-02DOI: 10.1186/s12952-016-0047-y
Mariana Herrera, Yudy Aguilar, Z. Rueda, C. Muskus, L. Vélez
{"title":"Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria","authors":"Mariana Herrera, Yudy Aguilar, Z. Rueda, C. Muskus, L. Vélez","doi":"10.1186/s12952-016-0047-y","DOIUrl":"https://doi.org/10.1186/s12952-016-0047-y","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0047-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-17DOI: 10.1186/s12952-016-0046-z
B. Olsen, D. Mielenz
{"title":"Thanks to all those who reviewed for Journal of Negative Results in BioMedicine in 2015","authors":"B. Olsen, D. Mielenz","doi":"10.1186/s12952-016-0046-z","DOIUrl":"https://doi.org/10.1186/s12952-016-0046-z","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0046-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-03DOI: 10.1186/s12952-016-0045-0
Min Liu, T. Lu, Fang Jing, S. Dang, Wei Zhang
{"title":"ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model","authors":"Min Liu, T. Lu, Fang Jing, S. Dang, Wei Zhang","doi":"10.1186/s12952-016-0045-0","DOIUrl":"https://doi.org/10.1186/s12952-016-0045-0","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0045-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-01DOI: 10.1186/s12952-016-0044-1
D. Haider, G. Lindner, M. Wolzt, A. Leichtle, G. Fiedler, T. Sauter, V. Fuhrmann, A. Exadaktylos
{"title":"Use of Diuretics is not associated with mortality in patients admitted to the emergency department: results from a cross-sectional study","authors":"D. Haider, G. Lindner, M. Wolzt, A. Leichtle, G. Fiedler, T. Sauter, V. Fuhrmann, A. Exadaktylos","doi":"10.1186/s12952-016-0044-1","DOIUrl":"https://doi.org/10.1186/s12952-016-0044-1","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-016-0044-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-23DOI: 10.1186/s12952-015-0043-7
G. Alexiou, C. Sioka
{"title":"Mobile phone use and risk for intracranial tumors","authors":"G. Alexiou, C. Sioka","doi":"10.1186/s12952-015-0043-7","DOIUrl":"https://doi.org/10.1186/s12952-015-0043-7","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0043-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-18DOI: 10.1186/s12952-015-0042-8
S. Acevedo-Acevedo, W. Crone
{"title":"Substrate stiffness effect and chromosome missegregation in hIPS cells","authors":"S. Acevedo-Acevedo, W. Crone","doi":"10.1186/s12952-015-0042-8","DOIUrl":"https://doi.org/10.1186/s12952-015-0042-8","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0042-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-02DOI: 10.1186/s12952-015-0040-x
M. Krantz, E. Havranek, R. Pereira, B. Beaty, P. Mehler, C. Long
{"title":"Effects of omega-3 fatty acids on arterial stiffness in patients with hypertension: a randomized pilot study","authors":"M. Krantz, E. Havranek, R. Pereira, B. Beaty, P. Mehler, C. Long","doi":"10.1186/s12952-015-0040-x","DOIUrl":"https://doi.org/10.1186/s12952-015-0040-x","url":null,"abstract":"","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":"14 9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0040-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65694679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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