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Journal of proteins and proteomics最新文献

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Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts’ treatment on HeLa cells Nothapodytes nimmonana (J. Graham)提取物对HeLa细胞的蛋白质组学和代谢组学分析
Journal of proteins and proteomics
Pub Date : 2020-03-01 DOI: 10.1007/s42485-020-00031-0
S. Gayakwad, Amey Shirolkar, S. Warkad, Shraddha Bharsakale, S. Gaidhani, S. Pawar
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引用次数: 1
Computational analysis of regulatory genes network pathways among devastating cancer diseases 毁灭性癌症疾病中调控基因网络通路的计算分析
Journal of proteins and proteomics
Pub Date : 2020-03-01 DOI: 10.1007/s42485-020-00032-z
Tasnimul Alam Taz, Md. Kawsar, B. K. Paul, K. Ahmed
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引用次数: 1
Natural vs. random protein sequences: the novel neural network approach based on time series analysis 自然与随机蛋白质序列:基于时间序列分析的新型神经网络方法
Journal of proteins and proteomics
Pub Date : 2020-03-01 DOI: 10.1007/s42485-020-00029-8
A. Tsygvintsev
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引用次数: 0
Mass spectrometry-based identification and characterization of human hypothetical proteins highlighting the inconsistency across the protein databases 基于质谱的人类假设蛋白质的鉴定和表征突出了蛋白质数据库的不一致性
Journal of proteins and proteomics
Pub Date : 2020-03-01 DOI: 10.1007/s42485-020-00030-1
Johny Ijaq, N. Bethi, M. Jagannadham
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引用次数: 4
A comprehensive overview of proteomics approach for COVID 19: new perspectives in target therapy strategies. COVID - 19蛋白质组学方法综述:靶向治疗策略的新视角
Journal of proteins and proteomics
Pub Date : 2020-01-01 Epub Date: 2020-11-02 DOI: 10.1007/s42485-020-00052-9
Rashmi Rana, Vaishnavi Rathi, Nirmal Kumar Ganguly

World Health Organisation declared COVID-19 a pandemic on March 11, 2020. It was temporarily named as 2019-nCoV then subsequently named as COVID-19 virus. A coronavirus is a group of viruses, known to be zoonotic, causing illness ranging from acute to mild respiratory infections. These are spherical or pleomorphic enveloped particles containing positive sense RNA. The virus enters host cells, its uncoated genetic material transcribes, and translates. Since it has started spreading rapidly, protective measures have been taken all over the world. However, its transmission has been proved to be unstoppable and the absence of an effective drug makes the situation worse. The scientific community has gone all-out to discover and develop a possible vaccine or a competent antiviral drug. Other domains of biological sciences that promise effective results and target somewhat stable entities that are proteins, could be very useful in this time of crisis. Proteomics and metabolomics are the vast fields that are equipped with sufficient technologies to face this challenge. Various protein separation and identification techniques are available which facilitates the analysis of various types of interactions among proteins and their evolutionary lineages. The presented review aims at confronting the question: 'how proteomics can help in tackling SARS-CoV-2?' It deals with the role of upcoming proteome technology in these pandemic situations and discusses the proteomics approach towards the COVID-19 dilemma.

世界卫生组织于2020年3月11日宣布COVID-19为大流行。它被暂时命名为2019-nCoV，随后被命名为COVID-19病毒。冠状病毒是一组已知的人畜共患病毒，可引起从急性到轻度呼吸道感染不等的疾病。它们是球形或多形性包膜颗粒，含有正义RNA。病毒进入宿主细胞，其未包被的遗传物质进行转录和翻译。由于它开始迅速传播，世界各地都采取了保护措施。然而，它的传播已被证明是不可阻挡的，缺乏有效的药物使情况变得更糟。科学界已经全力以赴地发现和开发一种可能的疫苗或有效的抗病毒药物。生物科学的其他领域，承诺有效的结果，并针对一些稳定的实体，如蛋白质，可能在这个危机时期非常有用。蛋白质组学和代谢组学是一个广阔的领域，拥有足够的技术来应对这一挑战。目前已有多种蛋白质分离和鉴定技术，可用于分析蛋白质之间的各种相互作用及其进化谱系。这篇综述的目的是面对这样一个问题:“蛋白质组学如何帮助应对SARS-CoV-2?”它讨论了即将到来的蛋白质组技术在这些大流行情况下的作用，并讨论了蛋白质组学方法解决COVID-19困境。
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引用次数: 13
Virtualization of science education: a lesson from the COVID-19 pandemic. 科学教育虚拟化:2019冠状病毒病大流行的教训。
Journal of proteins and proteomics
Pub Date : 2020-01-01 Epub Date: 2020-05-31 DOI: 10.1007/s42485-020-00038-7
Sandipan Ray, Sanjeeva Srivastava

Nowadays, e-learning and virtual labs have gained substantial popularity in science education. Amid the COVID-19 shutdowns, regular in-person classroom teaching and lab courses are suspended in several countries worldwide. In this scenario, virtual classes and online resources could serve more effectively as a possible alternative way of learning science from home.

如今，电子学习和虚拟实验室在科学教育中得到了广泛的应用。在新冠肺炎疫情期间，全球多个国家暂停了常规的面对面课堂教学和实验课程。在这种情况下，虚拟课程和在线资源可以更有效地作为在家学习科学的一种可能的替代方式。
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引用次数: 67
A rapid and sensitive method to detect SARS-CoV-2 virus using targeted-mass spectrometry. 靶向质谱法快速灵敏检测SARS-CoV-2病毒
Journal of proteins and proteomics
Pub Date : 2020-01-01 Epub Date: 2020-08-31 DOI: 10.1007/s42485-020-00044-9
Praveen Singh, Rahul Chakraborty, Robin Marwal, V S Radhakrishan, Akash Kumar Bhaskar, Himanshu Vashisht, Mahesh S Dhar, Shalini Pradhan, Gyan Ranjan, Mohamed Imran, Anurag Raj, Uma Sharma, Priyanka Singh, Hemlata Lall, Meena Dutta, Parth Garg, Arjun Ray, Debasis Dash, Sridhar Sivasubbu, Hema Gogia, Preeti Madan, Sandhya Kabra, Sujeet K Singh, Anurag Agrawal, Partha Rakshit, Pramod Kumar, Shantanu Sengupta

In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by the novel coronavirus SARS-CoV-2 affecting millions of people worldwide. Early diagnosis and isolation are key to contain the rapid spread of the virus. Towards this goal, we report a simple, sensitive and rapid method to detect the virus using a targeted mass spectrometric approach, which can directly detect the presence of virus from naso-oropharyngeal swabs. Using a multiple reaction monitoring we can detect the presence of two peptides specific to SARS-CoV-2 in a 2.3 min gradient run with 100% specificity and 90.5% sensitivity when compared to RT-PCR. Importantly, we further show that these peptides could be detected even in the patients who have recovered from the symptoms and have tested negative for the virus by RT-PCR highlighting the sensitivity of the technique. This method has the translational potential of in terms of the rapid diagnostics of symptomatic and asymptomatic COVID-19 and can augment current methods available for diagnosis of SARS-CoV-2.

在过去的几个月里，由新型冠状病毒SARS-CoV-2引起的严重急性呼吸系统综合征在全球爆发了灾难性疫情，影响了全世界数百万人。早期诊断和隔离是遏制病毒快速传播的关键。为了实现这一目标，我们报告了一种简单、灵敏、快速的病毒检测方法，该方法使用靶向质谱法，可以直接检测鼻咽拭子中是否存在病毒。与RT-PCR相比，通过多反应监测，我们可以在2.3 min的梯度运行中检测到SARS-CoV-2特异性的两种肽的存在，特异性为100%，灵敏度为90.5%。重要的是，我们进一步表明，即使在症状恢复并通过RT-PCR检测病毒阴性的患者中也可以检测到这些肽，这突出了该技术的敏感性。该方法在快速诊断有症状和无症状的COVID-19方面具有转化潜力，并可以增强现有的诊断SARS-CoV-2的方法。
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引用次数: 41
Structural insights into the molecular mechanisms of pectinolytic enzymes 果胶分解酶分子机制的结构见解
Journal of proteins and proteomics
Pub Date : 2019-11-16 DOI: 10.1007/s42485-019-00027-5
Anuradha Kanungo, B. Bag
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引用次数: 6
In-silico molecular characterization and mutational analysis of inter-alpha-trypsin inhibitor heavy chain 4 in rheumatoid arthritis 类风湿关节炎中α -胰蛋白酶抑制剂重链4的硅基分子特征和突变分析
Journal of proteins and proteomics
Pub Date : 2019-11-05 DOI: 10.1007/s42485-019-00026-6
S. Khakha, SoundharaRajan G, Ankita Sharma, P. Kumari, S. Sahi, S. Biswas
{"title":"In-silico molecular characterization and mutational analysis of inter-alpha-trypsin inhibitor heavy chain 4 in rheumatoid arthritis","authors":"S. Khakha, SoundharaRajan G, Ankita Sharma, P. Kumari, S. Sahi, S. Biswas","doi":"10.1007/s42485-019-00026-6","DOIUrl":"https://doi.org/10.1007/s42485-019-00026-6","url":null,"abstract":"","PeriodicalId":73910,"journal":{"name":"Journal of proteins and proteomics","volume":"126 1","pages":"313 - 323"},"PeriodicalIF":0.0,"publicationDate":"2019-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77857689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acetogenins as potential checkpoint-2 kinase inhibitors: an in silico analysis 醋酸原蛋白作为潜在的检查点-2激酶抑制剂:计算机分析
Journal of proteins and proteomics
Pub Date : 2019-11-05 DOI: 10.1007/s42485-019-00025-7
P. Meghana, Jain R. Sandeep Kumar, T. Sandeep, N. Prashanth, H. Kumaraswamy
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引用次数: 2
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