{"title":"Correction.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40636396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yeremia Japhet Chuhila, Fred Demetrius Chibwana, Jestina Venance Katandukila, Chacha John Mwita
The Pangani catchment of Northern Tanzania harbours the critically endangered endemic tilapias of the genus Oreochromis. The introduction of non-native congenerics and consequent hybridization complicates taxa identification and phylogeny based on morphological systematics. We therefore morphologically and molecularly identified these tilapias and delimited their Molecular Operational Taxonomic Units (MOTUs) based on Cytochrome Oxidase Subunit I (CO1) gene for future management and conservation. A total of 132 indigenous and introduced tilapia specimens were morphologically identified, barcoded using the CO1 gene and delimited by Kimura 2 Parameter distance approaches, Automatic Barcode Gap Discovery (ABGD), Neighbour Joining (NJ) tree and haplotype analysis. Theoverall mean conspecific, congeneric and confamillial genetic distances based on the K2P model were 0.54%, 5.32% and 13.29% respectively. All taxa had a mean K2P distance < 2% and 90% (n = 10), were clearly delimited by the ABGD method. The NJ tree delimited tilapia taxa commensurate to the genetic distances depicted by DNA barcoding. However, DNA barcoding and NJ tree coherently failed to discriminate the morphologically distinct allopatric Oreochromis jipe and Oreochromis hunteri taxa. Moreover, the two methods depicted lack of monophyly in Oreochromis korogwe MOTUs implying that the taxon could consist of at least one MOTU. We conclude that the integration of morphological-based taxonomy and DNA barcoding among ichthyofaunal taxa herein will be invaluable in conservation and management of native tilapias in Pangani basin.
{"title":"DNA barcoding and delimitation of critically endangered indigenous and introduced tilapias (pisces cichlidae) of Pangani catchment, Northern Tanzania.","authors":"Yeremia Japhet Chuhila, Fred Demetrius Chibwana, Jestina Venance Katandukila, Chacha John Mwita","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Pangani catchment of Northern Tanzania harbours the critically endangered endemic tilapias of the genus <i>Oreochromis</i>. The introduction of non-native congenerics and consequent hybridization complicates taxa identification and phylogeny based on morphological systematics. We therefore morphologically and molecularly identified these tilapias and delimited their Molecular Operational Taxonomic Units (MOTUs) based on Cytochrome Oxidase Subunit I (CO1) gene for future management and conservation. A total of 132 indigenous and introduced tilapia specimens were morphologically identified, barcoded using the CO1 gene and delimited by Kimura 2 Parameter distance approaches, Automatic Barcode Gap Discovery (ABGD), Neighbour Joining (NJ) tree and haplotype analysis. Theoverall mean conspecific, congeneric and confamillial genetic distances based on the K2P model were 0.54%, 5.32% and 13.29% respectively. All taxa had a mean K2P distance < 2% and 90% (<i>n</i> = 10), were clearly delimited by the ABGD method. The NJ tree delimited tilapia taxa commensurate to the genetic distances depicted by DNA barcoding. However, DNA barcoding and NJ tree coherently failed to discriminate the morphologically distinct allopatric <i>Oreochromis jipe</i> and <i>Oreochromis hunteri</i> taxa. Moreover, the two methods depicted lack of monophyly in <i>Oreochromis korogwe</i> MOTUs implying that the taxon could consist of at least one MOTU. We conclude that the integration of morphological-based taxonomy and DNA barcoding among ichthyofaunal taxa herein will be invaluable in conservation and management of native tilapias in Pangani basin.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139076141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lamiophlomis rotata, the only species within the genus Lamiophlomis (family Labiatae), exhibits a broad geographical distribution in elevated highland areas in Qinghai-Tibetan Plateau and possesses significant therapeutic properties. Numerous chemical compositions and putative phylogenetic affiliations of this species have been documented in prior research. Nevertheless, there is a scarcity of accessible publications regarding the genomic data of L. rotata, particularly its chloroplast genome. This dearth of knowledge hampers the comprehensive investigation of its phylogenetic placement within the Labiatae family. In this study, we present a comprehensive analysis of the plastid genome of L. rotata. The plastid genome has a length of 151,837 base pairs (bp) and a GC content of 38.5%. Within this genome, a total of 135 genes were identified, including 90 protein-coding genes, 37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. By employing phylogenetic analysis, the taxonomic position of L. rotata within the family Labiatae is elucidated, highlighting a close relationship between the genus Lamiophlomis and the genus Phlomis. Notably, extensive genetic variations were uncovered between L. rotata and other Phlomis species. This study could provide significant insights for understanding the phylogenetic relationships of taxa within Labiatae.
{"title":"Complete chloroplast genome of <i>Lamiophlomis rotata</i>: comparative genome analysis and phylogenetic analysis.","authors":"Ji Wang, Xing-Xing Mao, Yazhen Ma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>Lamiophlomis rotata</i>, the only species within the genus <i>Lamiophlomis</i> (family Labiatae), exhibits a broad geographical distribution in elevated highland areas in Qinghai-Tibetan Plateau and possesses significant therapeutic properties. Numerous chemical compositions and putative phylogenetic affiliations of this species have been documented in prior research. Nevertheless, there is a scarcity of accessible publications regarding the genomic data of <i>L. rotata</i>, particularly its chloroplast genome. This dearth of knowledge hampers the comprehensive investigation of its phylogenetic placement within the Labiatae family. In this study, we present a comprehensive analysis of the plastid genome of <i>L. rotata</i>. The plastid genome has a length of 151,837 base pairs (bp) and a GC content of 38.5%. Within this genome, a total of 135 genes were identified, including 90 protein-coding genes, 37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. By employing phylogenetic analysis, the taxonomic position of <i>L. rotata</i> within the family Labiatae is elucidated, highlighting a close relationship between the genus <i>Lamiophlomis</i> and the genus <i>Phlomis</i>. Notably, extensive genetic variations were uncovered between <i>L. rotata</i> and other <i>Phlomis</i> species. This study could provide significant insights for understanding the phylogenetic relationships of taxa within Labiatae.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139378959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A total of 241 mitochondrial genomes were assembled and annotated from the SRA database to reconstruct a mtDNA genome phylogeny for the genus Drosophila, the family Drosophilidae, and close relatives. The resulting mtDNA genome phylogeny is largely congruent with previous higher-level analyses of Drosophila species with the exception of the relationships between the melanogaster, montium, anannassae, saltans and obscura groups. Although relationships within these species groups are congruent between nuclear and mtDNA studies, the mtDNA genome phylogeny of the groups is different when compared to earlier studies. Monophyly of known species groups within the genus Drosophila are highly supported and, as in previous work, the genera Lordiphosa, Hirtodrosophila, Zaprionus and Scaptomya are all imbedded within the genus Drosophila. Incongruence and partitioned support analyses indicate that DNA sequences are better at resolving the phylogeny than their translated protein sequences. Such analyses also indicate that genes on the minus strand of the circular molecule (Lrrna, Srrna, ND4, ND4L and ND5) provide most of the support for the overall phylogenetic hypothesis.
{"title":"Whole mitochondrial genome phylogeny of Drosophilidae.","authors":"Rob DeSalle, Sara Oppenheim, Patrick M O'Grady","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 241 mitochondrial genomes were assembled and annotated from the SRA database to reconstruct a mtDNA genome phylogeny for the genus <i>Drosophila</i>, the family Drosophilidae, and close relatives. The resulting mtDNA genome phylogeny is largely congruent with previous higher-level analyses of <i>Drosophila</i> species with the exception of the relationships between the melanogaster, montium, anannassae, saltans and obscura groups. Although relationships within these species groups are congruent between nuclear and mtDNA studies, the mtDNA genome phylogeny of the groups is different when compared to earlier studies. Monophyly of known species groups within the genus <i>Drosophila</i> are highly supported and, as in previous work, the genera <i>Lordiphosa</i>, <i>Hirtodrosophila</i>, <i>Zaprionus</i> and <i>Scaptomya</i> are all imbedded within the genus <i>Drosophila</i>. Incongruence and partitioned support analyses indicate that DNA sequences are better at resolving the phylogeny than their translated protein sequences. Such analyses also indicate that genes on the minus strand of the circular molecule (Lrrna, Srrna, ND4, ND4L and ND5) provide most of the support for the overall phylogenetic hypothesis.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139547593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We analyzed the mitochondrial DNA of Gekko hokouensis collected from the Izu Islands (maybe an introduced population) and the Nansei Islands (native population), both in Japan. A molecular phylogenetic analysis suggested that G. hokouensis of Japan belongs to a cryptic monophyletic group different from that of the currently discovered sample of China. Furthermore, the Japanese clade of G. hokouensis is differentiated into two subclades (Clade 1 and Clade 2 in this article). In the Nansei Islands, these two subclades form a complicated nested-distribution pattern and do not coexist on any of the islands, whereas both clades appear to coexist in the Izu Islands. The two clades exhibit high genetic diversity in the Nansei islands, which are the source population. Surprisingly, it has been revealed that high genetic diversity has also been maintained in the Izu Islands, which are the introduced population, in each clade. AMOVA has also revealed that the genetic differentiation between the populations in the Izu Islands and the Nansei Islands was not significant in each clade. These results suggest that the population of the Izu Islands is now in secondary contact between two clades by multiple migrations from various regions of the Nansei Islands.
{"title":"Secondary contact of two cryptic Hokou gecko groups in the Izu Islands, Japan.","authors":"Minoru Chiba, Daishi Yamazaki, Shun Ito, Osamu Kagawa, Satoshi Chiba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We analyzed the mitochondrial DNA of <i>Gekko hokouensis</i> collected from the Izu Islands (maybe an introduced population) and the Nansei Islands (native population), both in Japan. A molecular phylogenetic analysis suggested that <i>G. hokouensis</i> of Japan belongs to a cryptic monophyletic group different from that of the currently discovered sample of China. Furthermore, the Japanese clade of <i>G. hokouensis</i> is differentiated into two subclades (Clade 1 and Clade 2 in this article). In the Nansei Islands, these two subclades form a complicated nested-distribution pattern and do not coexist on any of the islands, whereas both clades appear to coexist in the Izu Islands. The two clades exhibit high genetic diversity in the Nansei islands, which are the source population. Surprisingly, it has been revealed that high genetic diversity has also been maintained in the Izu Islands, which are the introduced population, in each clade. AMOVA has also revealed that the genetic differentiation between the populations in the Izu Islands and the Nansei Islands was not significant in each clade. These results suggest that the population of the Izu Islands is now in secondary contact between two clades by multiple migrations from various regions of the Nansei Islands.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139577346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The giant trevallies (Caranx ignobilis) is a globally important fish species that is at risk from overexploitation. In this study, 150 C. ignobilis from six provinces in the Philippines were collected for genetic analyses. For each province, five representative specimens of C. ignobilis were subjected to DNA barcoding and revealed high interspecific K2P distances of 9.58% and 17.29% when compared to other species of Caranx and Carangoides, respectively. On the other hand, all 150 C. ignobilis specimens were subjected to population genetic analysis using the mitochondrial cytochrome b region. In the studied population of C. ignobilis, 33 unique haplotypes were observed, and the population exhibited high haplotype (h = 0.831) and nucleotide (π=0.930%) diversity. Pairwise FST values between the six study sites indicated limited genetic differentiation among the studied populations. The limited genetic differentiation may be due to the oceanic currents in the Philippines facilitating larval dispersal as observed in the results of the Lagrangian dispersion model. Data from neutrality tests, mismatch distribution, and Bayesian skyline plot revealed that the population may have undergone demographic expansion. This study provides valuable genetic information on C. ignobilis that can be used for formulating sustainable fishery management strategies.
巨鲹(Caranx ignobilis)是全球重要的鱼类物种,正面临过度开发的风险。在这项研究中,我们从菲律宾的六个省份收集了 150 条巨无霸鱼进行基因分析。在每个省份中,对 5 个具有代表性的 C. ignobilis 标本进行了 DNA 条形码分析,结果显示,与 Caranx 和 Carangoides 的其他物种相比,C. ignobilis 的种间 K2P 距离分别高达 9.58% 和 17.29%。另一方面,利用线粒体细胞色素 b 区对所有 150 个 C. ignobilis 标本进行了种群遗传分析。在所研究的 C. ignobilis 种群中,观察到 33 个独特的单倍型,该种群表现出较高的单倍型(h = 0.831)和核苷酸(π=0.930%)多样性。六个研究地点之间的配对 FST 值表明,研究种群之间的遗传分化有限。遗传分化有限的原因可能是菲律宾的洋流促进了幼虫的扩散,拉格朗日扩散模型的结果也证明了这一点。来自中性检验、错配分布和贝叶斯天际线图的数据显示,该种群可能经历了人口扩张。这项研究为制定可持续渔业管理策略提供了宝贵的 C. ignobilis 遗传信息。
{"title":"Genetic diversity and population structure of giant trevallies (<i>Caranx ignobilis</i> Forsskål, 1775) in the Philippines with implications to management.","authors":"Shenna Kate M Torres, Brian S Santos","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The giant trevallies (<i>Caranx ignobilis</i>) is a globally important fish species that is at risk from overexploitation. In this study, 150 <i>C. ignobilis</i> from six provinces in the Philippines were collected for genetic analyses. For each province, five representative specimens of <i>C. ignobilis</i> were subjected to DNA barcoding and revealed high interspecific K2P distances of 9.58% and 17.29% when compared to other species of <i>Caranx</i> and <i>Carangoides</i>, respectively. On the other hand, all 150 <i>C. ignobilis</i> specimens were subjected to population genetic analysis using the mitochondrial cytochrome b region. In the studied population of <i>C. ignobilis</i>, 33 unique haplotypes were observed, and the population exhibited high haplotype (<i>h</i> = 0.831) and nucleotide (π=0.930%) diversity. Pairwise F<sub>ST</sub> values between the six study sites indicated limited genetic differentiation among the studied populations. The limited genetic differentiation may be due to the oceanic currents in the Philippines facilitating larval dispersal as observed in the results of the Lagrangian dispersion model. Data from neutrality tests, mismatch distribution, and Bayesian skyline plot revealed that the population may have undergone demographic expansion. This study provides valuable genetic information on <i>C. ignobilis</i> that can be used for formulating sustainable fishery management strategies.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139076142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40624640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The nine endemic species of the genus Schizothorax from the Yarlung Zangbo River, Tibet comprise a putative cyprinid species flock. In this study, the effectiveness of the COI DNA barcode for Schizothoracinae species identification was verified by using 45 COI sequences covering nine species in four genera of Schizothoracinae fishes. The average Kimura two parameter (K2P) genetic distances within and among species were 0.13% and 8.57%, respectively. The results revealed that most of the species were clearly discriminated by their estimated genetic distances and monophyletic clustering in a maximum likelihood tree. However, insignificant genetic distances were noticed in two reportedly valid species: Schizothorax molesworthi and S. integrilabiatus (0.1%), and the monophyly of S. macropogon could not be recovered in the schizothoracine group. The fishes of S.curilabiatus living in the lower course of Yalung Zangbo River were clustered together with three species from the upper course, which is inconsistent with the geographical distribution of the populations.
西藏雅鲁藏布江五棘鱼属的九个特有种组成了一个假定的鲤科鱼种群。本研究利用 45 条 COI 序列验证了 COI DNA 条形码在五棘鱼科物种鉴定中的有效性,这些序列涵盖了五棘鱼科 4 属 9 个物种。种内和种间的平均木村两参数(K2P)遗传距离分别为 0.13% 和 8.57%。研究结果表明,大多数鱼种都能通过估计的遗传距离和最大似然树中的单系聚类进行明显区分。然而,有两个据报告有效的物种的遗传距离不显著:S. macropogon 的单系在裂腹鱼类中无法恢复。生活在雅鲁藏布江下游的S.curilabiatus与上游的3个物种聚集在一起,这与种群的地理分布不一致。
{"title":"DNA barcoding of Schizothoracinae fishes from the Yarlung Zangbo River in Tibet.","authors":"Chi Zhang, Liying Sui, Xuekai Han","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The nine endemic species of the genus <i>Schizothorax</i> from the Yarlung Zangbo River, Tibet comprise a putative cyprinid species flock. In this study, the effectiveness of the COI DNA barcode for Schizothoracinae species identification was verified by using 45 COI sequences covering nine species in four genera of Schizothoracinae fishes. The average Kimura two parameter (K2P) genetic distances within and among species were 0.13% and 8.57%, respectively. The results revealed that most of the species were clearly discriminated by their estimated genetic distances and monophyletic clustering in a maximum likelihood tree. However, insignificant genetic distances were noticed in two reportedly valid species: <i>Schizothorax molesworthi</i> and <i>S. integrilabiatus</i> (0.1%), and the monophyly of <i>S. macropogon</i> could not be recovered in the schizothoracine group. The fishes of <i>S</i>.<i>curilabiatus</i> living in the lower course of Yalung Zangbo River were clustered together with three species from the upper course, which is inconsistent with the geographical distribution of the populations.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139081134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samara Videira Zorzato, Amir Yassin, Lilian Madi-Ravazzi
Pleistocene climatic changes have played a major role in the evolution of Brazilian Atlantic Forest and South America biodiversity but their impacts on the genetic structure of widely distributed species remain unclear. Here, we investigate mitochondrial DNA (mtDNA) diversity in 21 geographical populations of Drosophila sturtevanti, Nucleotide sequences of the cytochrome C oxidase subunits I and II genes (COI and COII, respectively) from 163 individuals, showed a significant north-south structure, in spite of an overall low level of variation. The haplotypes clustered in three groups that showed strong correlations with geographical and climatic variables, suggesting that local adaptations might have contributed to differentiation within the species. Coalescent-based analyses indicated that the three clusters have differentiated nearly ∼17.000 years ago, suggesting a major role for Pleistocene changes in shaping current day distributions and differentiation of widespread Neotropical species.
{"title":"Signature of climatic differentiation on mitochondrial DNA of <i>Drosophila sturtevanti</i>.","authors":"Samara Videira Zorzato, Amir Yassin, Lilian Madi-Ravazzi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pleistocene climatic changes have played a major role in the evolution of Brazilian Atlantic Forest and South America biodiversity but their impacts on the genetic structure of widely distributed species remain unclear. Here, we investigate mitochondrial DNA (mtDNA) diversity in 21 geographical populations of <i>Drosophila sturtevanti</i>, Nucleotide sequences of the cytochrome C oxidase subunits I and II genes (<i>COI</i> and <i>COII</i>, respectively<i>)</i> from 163 individuals, showed a significant north-south structure, in spite of an overall low level of variation. The haplotypes clustered in three groups that showed strong correlations with geographical and climatic variables, suggesting that local adaptations might have contributed to differentiation within the species. Coalescent-based analyses indicated that the three clusters have differentiated nearly ∼17.000 years ago, suggesting a major role for Pleistocene changes in shaping current day distributions and differentiation of widespread Neotropical species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39778504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yılmaz Çiftci, Oğuzhan Eroğlu, Şirin Firidin, Hacı Savaş, Yusuf Bektaş
Genetic diversity, genetic structure, and demographic history of the endemic and endangered cyprinid species Alburnus tarichi based on samples from 17 populations consisting of resident and potamodromous specimens from the Lake Van basin in eastern Turkey were analyzed using two mitochondrial DNA markers. A. tarichi populations in the Lake Van basin are genetically heterogeneous, as indicated by the high haplotype and low nucleotide diversity of 1233 bp of the 16S rRNA marker (44 haplotypes; 70 polymorphic sites, haploid diversity (Hd) = 0.9130, π = 0.0032) and 1140 bp of the cyt b marker (47 haplotypes; 82 polymorphic sites, Hd = 0.9339, π = 0.0057). Clades were separated by average sequence divergences of 1.94% (II vs. III), 1.80% (I vs. III), and 0.66% (I vs. II). Based on these clades, AMOVA analysis revealed that 80.76% of the total variation occurred among populations, 10.74% occurred within populations, and only 8.50% occurred between populations within groups for the concatenated 16S rRNA-cyt b sequences. Pairwise FST values varied from 0.0167 to 0.9705 for the concatenated 16S rRNA-cyt b dataset, emphasizing the high genetic variation among populations. The time since the endemic tarek populations split from their last common ancestor has been dated to 5.647 Ma (95% highest posterior density: 4.183-7.011 Ma) in the Messinian Stage. Recent population expansion for tarek populations has been determined by neutrality tests and mismatch distribution analyses. The results of this study provide valuable information on the genetic population structure, conservation, and management of this species.
{"title":"Genetic structure and demographic history of endangered <i>Alburnus tarichi</i> (Güldenstädt, 1814) populations from Lake Van basin in Turkey inferred from mtDNA analyses.","authors":"Yılmaz Çiftci, Oğuzhan Eroğlu, Şirin Firidin, Hacı Savaş, Yusuf Bektaş","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genetic diversity, genetic structure, and demographic history of the endemic and endangered cyprinid species <i>Alburnus tarichi</i> based on samples from 17 populations consisting of resident and potamodromous specimens from the Lake Van basin in eastern Turkey were analyzed using two mitochondrial DNA markers. <i>A. tarichi</i> populations in the Lake Van basin are genetically heterogeneous, as indicated by the high haplotype and low nucleotide diversity of 1233 bp of the 16S rRNA marker (44 haplotypes; 70 polymorphic sites, haploid diversity (<i>H<sub>d</sub></i>) = 0.9130, π = 0.0032) and 1140 bp of the cyt b marker (47 haplotypes; 82 polymorphic sites, <i>H<sub>d</sub></i> = 0.9339, π = 0.0057). Clades were separated by average sequence divergences of 1.94% (II vs. III), 1.80% (I vs. III), and 0.66% (I vs. II). Based on these clades, AMOVA analysis revealed that 80.76% of the total variation occurred among populations, 10.74% occurred within populations, and only 8.50% occurred between populations within groups for the concatenated 16S rRNA-cyt b sequences. Pairwise <i>F<sub>ST</sub></i> values varied from 0.0167 to 0.9705 for the concatenated 16S rRNA-cyt b dataset, emphasizing the high genetic variation among populations. The time since the endemic tarek populations split from their last common ancestor has been dated to 5.647 Ma (95% highest posterior density: 4.183-7.011 Ma) in the Messinian Stage. Recent population expansion for tarek populations has been determined by neutrality tests and mismatch distribution analyses. The results of this study provide valuable information on the genetic population structure, conservation, and management of this species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39937743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}