Pub Date : 2025-01-01Epub Date: 2025-03-21DOI: 10.1186/s44280-025-00070-6
Qingkui Jiang, Ranjeet Kumar, Yi Zhao, Selvakumar Subbian, Lanbo Shi
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a global health challenge. Arginine metabolism is central to immune responses, regulating nitric oxide (NO) production via inducible NO synthase (Nos2) and competing pathways mediated by arginases (Arg1 and Arg2). This study examines the impact of arginine supplementation and arginase inhibition during the acute phase of Mtb infection in mouse lungs, focusing on immune function, lung pathology, and mitochondrial function. Arginine supplementation enhanced Nos2 expression, promoted mitophagy, and supported angiogenesis and/or tissue repair by upregulating Vegfa. These mechanisms synergized to balance pro-inflammatory responses with tissue repair, improving immune defense while mitigating lung damage. In contrast, arginase inhibition disrupted Vegfa-mediated immune homeostasis, and impaired mitophagy, leading to exacerbated lung pathology. These findings underscore the complementary roles of Nos2 and arginase-mediated pathways in maintaining immune equilibrium during Mtb infection. Our results highlight arginine supplementation as a promising host-directed therapy for TB, capable of enhancing protective immunity and facilitating tissue repair. Conversely, caution is warranted for strategies targeting arginase due to potential adverse effects on inflammation resolution and mitochondrial quality control. Future studies should explore the long-term efficacy of arginine-based therapies and their integration with existing antibiotic regimens for optimal TB management.
Supplementary information: The online version contains supplementary material available at 10.1186/s44280-025-00070-6.
{"title":"Arginine as host directed therapy in tuberculosis: insights from modulating arginine metabolism by supplementation and arginase inhibition.","authors":"Qingkui Jiang, Ranjeet Kumar, Yi Zhao, Selvakumar Subbian, Lanbo Shi","doi":"10.1186/s44280-025-00070-6","DOIUrl":"10.1186/s44280-025-00070-6","url":null,"abstract":"<p><p>Tuberculosis (TB), caused by <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>), remains a global health challenge. Arginine metabolism is central to immune responses, regulating nitric oxide (NO) production via inducible NO synthase (Nos2) and competing pathways mediated by arginases (Arg1 and Arg2). This study examines the impact of arginine supplementation and arginase inhibition during the acute phase of <i>Mtb</i> infection in mouse lungs, focusing on immune function, lung pathology, and mitochondrial function. Arginine supplementation enhanced Nos2 expression, promoted mitophagy, and supported angiogenesis and/or tissue repair by upregulating <i>Vegfa</i>. These mechanisms synergized to balance pro-inflammatory responses with tissue repair, improving immune defense while mitigating lung damage. In contrast, arginase inhibition disrupted <i>Vegfa</i>-mediated immune homeostasis, and impaired mitophagy, leading to exacerbated lung pathology. These findings underscore the complementary roles of Nos2 and arginase-mediated pathways in maintaining immune equilibrium during <i>Mtb</i> infection. Our results highlight arginine supplementation as a promising host-directed therapy for TB, capable of enhancing protective immunity and facilitating tissue repair. Conversely, caution is warranted for strategies targeting arginase due to potential adverse effects on inflammation resolution and mitochondrial quality control. Future studies should explore the long-term efficacy of arginine-based therapies and their integration with existing antibiotic regimens for optimal TB management.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44280-025-00070-6.</p>","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patulin (PTL, 154 Da) is a small molecule toxin produced as a mold metabolite. PTL is unstable in animals and is quickly converted to its metabolites. Thus, few successful antibodies were reported even after extensive efforts in hapten design. In this work, a library immobilization strategy was used to obtain DNA aptamers for PTL, where exposure of PTL to live animals or cells is no longer needed. After 20 rounds of selection, the most abundant aptamer named PTL-1 has a dissociation constant (Kd) of 12.5 μM based on isothermal titration calorimetry and 18.4 μM based on thioflavin T (ThT) fluorescence spectroscopy. PTL-1 has excellent selectivity against nine other antibiotics. Compared to previously reported aptamers for PTL, PTL-1 is either shorter or has higher affinity. A label-free ThT fluorescence assay based on PTL-1 was developed, which has a detection limit of 0.2 μM (detection range 0.2-20 μM) for PTL in fruit juices. The PTL-1 aptamer shows resistance to matrix interference and can be applied to the rapid detection of PTL in a blended juice. This study is a nice demonstration of selecting aptamers for a target molecule difficult for the production of antibodies.
Graphical abstract:
Supplementary information: The online version contains supplementary material available at 10.1186/s44280-025-00101-2.
{"title":"Selection and characterization of a DNA aptamer for Patulin and its application in a label-free fluorescence assay for fruit juices.","authors":"Sihan Wang, Jiayi Liang, Haiyang Jiang, Jianzhong Shen, Zhanhui Wang, Juewen Liu","doi":"10.1186/s44280-025-00101-2","DOIUrl":"10.1186/s44280-025-00101-2","url":null,"abstract":"<p><p>Patulin (PTL, 154 Da) is a small molecule toxin produced as a mold metabolite. PTL is unstable in animals and is quickly converted to its metabolites. Thus, few successful antibodies were reported even after extensive efforts in hapten design. In this work, a library immobilization strategy was used to obtain DNA aptamers for PTL, where exposure of PTL to live animals or cells is no longer needed. After 20 rounds of selection, the most abundant aptamer named PTL-1 has a dissociation constant (<i>K</i> <sub>d</sub>) of 12.5 μM based on isothermal titration calorimetry and 18.4 μM based on thioflavin T (ThT) fluorescence spectroscopy. PTL-1 has excellent selectivity against nine other antibiotics. Compared to previously reported aptamers for PTL, PTL-1 is either shorter or has higher affinity. A label-free ThT fluorescence assay based on PTL-1 was developed, which has a detection limit of 0.2 μM (detection range 0.2-20 μM) for PTL in fruit juices. The PTL-1 aptamer shows resistance to matrix interference and can be applied to the rapid detection of PTL in a blended juice. This study is a nice demonstration of selecting aptamers for a target molecule difficult for the production of antibodies.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44280-025-00101-2.</p>","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":"37"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145866808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-24DOI: 10.1186/s44280-024-00049-9
G. Oliveri Conti, Paola Rapisarda, M. Ferrante
{"title":"Relationship between climate change and environmental microplastics: a one health vision for the platysphere health","authors":"G. Oliveri Conti, Paola Rapisarda, M. Ferrante","doi":"10.1186/s44280-024-00049-9","DOIUrl":"https://doi.org/10.1186/s44280-024-00049-9","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141099665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meta-analysis on the global prevalence of Arcobacter in food-producing animals and humans","authors":"Penghang Zhang, Yuzhu Liu, Mengjiao Fu, Bing Wang, Shuangyang Ding, Xiaochen Ma, Xiaoai Zhang, Zhangqi Shen","doi":"10.1186/s44280-024-00046-y","DOIUrl":"https://doi.org/10.1186/s44280-024-00046-y","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140992945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-09DOI: 10.1186/s44280-024-00050-2
Yi Yin, Kai Peng, Y. Li, Wenhui Zhang, Yanyun Gao, Xinran Sun, Sheng Chen, Zhiqiang Wang, Ruichao Li
{"title":"Transmission patterns of multiple strains producing New Delhi metallo-β-lactamase variants among animals and the environment in live poultry markets","authors":"Yi Yin, Kai Peng, Y. Li, Wenhui Zhang, Yanyun Gao, Xinran Sun, Sheng Chen, Zhiqiang Wang, Ruichao Li","doi":"10.1186/s44280-024-00050-2","DOIUrl":"https://doi.org/10.1186/s44280-024-00050-2","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":" 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140995504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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