Pub Date : 2023-09-27DOI: 10.1186/s44280-023-00025-9
Feiyu Yu, Haijie Zhang, Shuyao Zhu, Zhiqiang Wang, Yuan Liu
Abstract The emergence and prevalence of plasmid-encoded RND-type efflux pump TMexCD-TOprJ severely compromise tigecycline treatment, which is recognized as the last resort for multidrug-resistant (MDR) Gram-negative bacterial infections. There is an urgent need for rapid antibiotic susceptibility testing (AST) that can simultaneously identify the genotype and phenotype of tmexCD-toprJ -positive bacteria. Through characterizing transcriptional profiling responses of tmexCD-toprJ -positive and -negative strains after exposure to 2 μg/mL tigecycline, here we identified 12 differentially RNA biomarkers and developed an RNA-based AST (RBAST) to distinguish tmexCD-toprJ -positive and -negative K. pneumoniae . These mRNA biomarkers were successfully validated in tigecycline exposure time variations, concentration shifts, and other tmexCD-toprJ variants. In addition, a group of clinical isolated strains was effectively distinguished using RBAST, with an accuracy of over 94% during 3 h test period. Our work highlights the potential of RNA transcripts as biomarkers for rapid AST, which will contribute to deploying effective antibiotic regimens in clinical practice.
{"title":"RNA-based antibiotic susceptibility testing of tmexCD-toprJ-mediated tigecycline resistance in Klebsiella pneumoniae","authors":"Feiyu Yu, Haijie Zhang, Shuyao Zhu, Zhiqiang Wang, Yuan Liu","doi":"10.1186/s44280-023-00025-9","DOIUrl":"https://doi.org/10.1186/s44280-023-00025-9","url":null,"abstract":"Abstract The emergence and prevalence of plasmid-encoded RND-type efflux pump TMexCD-TOprJ severely compromise tigecycline treatment, which is recognized as the last resort for multidrug-resistant (MDR) Gram-negative bacterial infections. There is an urgent need for rapid antibiotic susceptibility testing (AST) that can simultaneously identify the genotype and phenotype of tmexCD-toprJ -positive bacteria. Through characterizing transcriptional profiling responses of tmexCD-toprJ -positive and -negative strains after exposure to 2 μg/mL tigecycline, here we identified 12 differentially RNA biomarkers and developed an RNA-based AST (RBAST) to distinguish tmexCD-toprJ -positive and -negative K. pneumoniae . These mRNA biomarkers were successfully validated in tigecycline exposure time variations, concentration shifts, and other tmexCD-toprJ variants. In addition, a group of clinical isolated strains was effectively distinguished using RBAST, with an accuracy of over 94% during 3 h test period. Our work highlights the potential of RNA transcripts as biomarkers for rapid AST, which will contribute to deploying effective antibiotic regimens in clinical practice.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135536990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.1186/s44280-023-00022-y
Qianyu Jia, Yu Wang, Haidong Pang, Kai Fan, Huisheng Xie, Jiahao Lin
Abstract Thoracolumbar interverbal disk herniation (TL-IVDH) is a common neurological disorder in dogs, which often affects the life quality of patients and their caregivers. Acupuncture is increasingly accepted by veterinarians and animal owners, and has been used in the treatment of neurologic disorders. This study aims to evaluate the effects of acupuncture treatment for canine TL-IVDH. Clinical cases from 2016 to 2021 were collected. According to the inclusion and exclusion criteria, 94 TL-IVDH cases that received acupuncture treatment were selected. Clinical classification and efficacy criteria were formulated based on the Frankel Score. For each case, clinical presentation, improvement, recovery and recurrence were recorded. Male dogs with TL-IVDH accounted for 64% of all cases. The average age was 7.49 years old, the predominant breed was poodle, and the incidence site was concentrated from T11 to L1. The overall recovery rate after receiving acupuncture treatment was 79.78%, which was comparable when considering only cases with grade 2, 3, or 4 spinal cord lesions (83.33% ~ 85.19%). In conclusion, canine with grade 2, 3, and 4 spinal cord lesions due to TL-IVDH receiving acupuncture had a high recovery rate, suggesting acupuncture could be a viable treatment options for canine with TL-IVDH.
{"title":"Retrospective study of acupuncture treatment for canine thoracolumbar intervertebral disc herniation","authors":"Qianyu Jia, Yu Wang, Haidong Pang, Kai Fan, Huisheng Xie, Jiahao Lin","doi":"10.1186/s44280-023-00022-y","DOIUrl":"https://doi.org/10.1186/s44280-023-00022-y","url":null,"abstract":"Abstract Thoracolumbar interverbal disk herniation (TL-IVDH) is a common neurological disorder in dogs, which often affects the life quality of patients and their caregivers. Acupuncture is increasingly accepted by veterinarians and animal owners, and has been used in the treatment of neurologic disorders. This study aims to evaluate the effects of acupuncture treatment for canine TL-IVDH. Clinical cases from 2016 to 2021 were collected. According to the inclusion and exclusion criteria, 94 TL-IVDH cases that received acupuncture treatment were selected. Clinical classification and efficacy criteria were formulated based on the Frankel Score. For each case, clinical presentation, improvement, recovery and recurrence were recorded. Male dogs with TL-IVDH accounted for 64% of all cases. The average age was 7.49 years old, the predominant breed was poodle, and the incidence site was concentrated from T11 to L1. The overall recovery rate after receiving acupuncture treatment was 79.78%, which was comparable when considering only cases with grade 2, 3, or 4 spinal cord lesions (83.33% ~ 85.19%). In conclusion, canine with grade 2, 3, and 4 spinal cord lesions due to TL-IVDH receiving acupuncture had a high recovery rate, suggesting acupuncture could be a viable treatment options for canine with TL-IVDH.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"173 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135015920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.1186/s44280-023-00020-0
Jiajia Zhu, Yunsheng Chen, K. Imre, D. Arslan-Acaroz, F. R. Istanbullugil, Yuwen Fang, G. Ros, Kui Zhu, Ulaș Acaroz
{"title":"Mechanisms of probiotic Bacillus against enteric bacterial infections","authors":"Jiajia Zhu, Yunsheng Chen, K. Imre, D. Arslan-Acaroz, F. R. Istanbullugil, Yuwen Fang, G. Ros, Kui Zhu, Ulaș Acaroz","doi":"10.1186/s44280-023-00020-0","DOIUrl":"https://doi.org/10.1186/s44280-023-00020-0","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76248643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-14DOI: 10.1186/s44280-023-00021-z
Pingping Gao, S. Shi, Di Zhang, Yueping Zhang
{"title":"Rapid and visual RPA-CRISPR/Cas12a detection for Staphylococcus pseudintermedius and methicillin-resistant S. pseudintermedius in clinical samples of dogs and cats","authors":"Pingping Gao, S. Shi, Di Zhang, Yueping Zhang","doi":"10.1186/s44280-023-00021-z","DOIUrl":"https://doi.org/10.1186/s44280-023-00021-z","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89260025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1186/s44280-023-00016-w
Liang Zhang, Sihan Wang, J. Xiong, Yuliang Xv, Jie Xie, Zhanhui Wang, Congming Wu, Haiyang Jiang, Jianzhong Shen
{"title":"A free elution immunoassay for chloramphenicol in chicken and egg after magnetic ZIF-8 adsorption separation","authors":"Liang Zhang, Sihan Wang, J. Xiong, Yuliang Xv, Jie Xie, Zhanhui Wang, Congming Wu, Haiyang Jiang, Jianzhong Shen","doi":"10.1186/s44280-023-00016-w","DOIUrl":"https://doi.org/10.1186/s44280-023-00016-w","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"333 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77631655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1186/s44280-023-00017-9
Kaichao Chen, Miaomiao Xie, Han Wang, E. Chan, Sheng Chen
{"title":"Intercontinental spread and clonal expansion of ColRNA1 plasmid-bearing Salmonella Corvallis ST1541 strains: a genomic epidemiological study","authors":"Kaichao Chen, Miaomiao Xie, Han Wang, E. Chan, Sheng Chen","doi":"10.1186/s44280-023-00017-9","DOIUrl":"https://doi.org/10.1186/s44280-023-00017-9","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80662481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：