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Genetic background of body reserves in laying hens through backfat thickness phenotyping 通过背膘厚度表型分析蛋鸡体储的遗传背景
Pub Date : 2024-04-04 DOI: 10.24072/pcjournal.412
N. Bedere, Joëlle Dupont, Y. Baumard, Christophe Staub, D. Gourichon, Elleboudt Frédéric, Pascale Le Roy, Tatiana Zerjal
In this study, we pursued three primary objectives: firstly to test and validate the pheno-typing of backfat thickness as an indicator of the overall fatness of laying hens; secondly, to estimate genetic parameters for this trait; thirdly, to study the phenotypic and genetic relationships between this trait and other traits related to production and body composition. To address these questions, hens from two lines under divergent selection for residual feed intake, were phenotyped for body weight, body composition traits (backfat, total fat volume, and blood adipokines levels), and egg number. Linear mixed models enabled to estimate variance components and calculate
在这项研究中,我们有三个主要目标:首先,测试和验证背膘厚度作为蛋鸡整体肥胖指标的表型;其次,估计该性状的遗传参数;第三,研究该性状与其他与生产和身体组成相关的性状之间的表型和遗传关系。为了解决这些问题,对两个品系的母鸡进行了体重、体成分性状(背脂、总脂肪量和血液脂肪因子水平)和蛋数的表型分析。线性混合模型可以估计方差成分并计算
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引用次数: 0
Dealing with post-excavation data: the Omeka S TiMMA web-database 处理发掘后数据:Omeka S TiMMA 网络数据库
Pub Date : 2024-01-24 DOI: 10.24072/pcjournal.366
Bastien Rueff
This paper reports on the creation and use of a web database designed as part of the TiMMA project with the Content Management System Omeka S. Rather than resulting in a technical manual, its goal is to analyze the relevance of using Omeka S in the frame of a post-excavation project, gathering a wide team of researchers from several countries, working in their own language and having their own specialty. Designed to assist organizations and institutions in creating and managing digital collections, Omeka S offers a number of commodities from the perspective of both the administrator(s) and the users, the most significant being its efficiency in capitalizing on linked data standards for items description. Additionally, one of the successful achievements of this platform is to benefit the open-science tools more broadly, permitting for instance, as was done in this project, to import images with the iiiF API and bibliographical references with the Zotero module, thus avoiding the creation of new digital files and metadata. Designed with simplicity in mind, the graphic interface of Omeka S makes it a particularly appropriate tool for
本文介绍了在 TiMMA 项目中使用内容管理系统 Omeka S 创建和使用网络数据库的情况。本文的目的不是编写技术手册,而是分析在发掘后项目框架内使用 Omeka S 的相关性,该项目汇集了来自多个国家的研究人员,他们使用各自的语言和专业开展工作。Omeka S 的设计目的是帮助组织和机构创建和管理数字藏品,从管理员和用户的角度来看,它提供了许多商品,其中最重要的是它在利用链接数据标准进行物品描述方面的便利性。此外,该平台的成功之处还在于为开放科学工具提供了更广泛的帮助,例如,在本项目中,可以使用 iiiF API 导入图像,使用 Zotero 模块导入参考文献,从而避免创建新的数字文件和元数据。Omeka S 的图形界面以简洁为设计理念,使其成为特别适合以下用途的工具
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引用次数: 0
Mark loss can strongly bias estimates of demographic rates in multi-state models: a case study with simulated and empirical datasets 标记丢失会对多州模型中的人口统计率估算造成严重偏差:利用模拟数据集和经验数据集进行的案例研究
Pub Date : 2023-12-21 DOI: 10.24072/pcjournal.348
F. Touzalin, Eric J. Petit, Emmanuelle Cam, Claire Stagier, E. Teeling, S. Puechmaille
1. The development of methods for individual identification in wild species and the refinement of Capture-Mark-Recapture (CMR) models over the past few decades have greatly improved the assessment of population demographic rates to address ecological and conservation questions. In particular, multi-state models, which offer flexibility in analysing complex study systems, have gained popularity within the ecological community. In this study, we focus on the issue of mark loss and the associated recycling of remarked individuals, which requires further exploration given the increasing use of these models. 2. To fill this knowledge gap, we employed a wide range of simulation scenarios that reflect commonly encountered real case studies, drawing inspiration from the
1.过去几十年来,野生物种个体识别方法的发展和捕获-标记-再捕获(CMR)模型的改进极大地改善了对种群人口率的评估,从而解决了生态和保护问题。尤其是多态模型,它在分析复杂的研究系统方面具有很强的灵活性,在生态学界越来越受欢迎。在本研究中,我们将重点关注标记丢失问题以及与之相关的已注释个体的回收问题。2.为了填补这一知识空白,我们采用了多种模拟情景,这些情景反映了常见的实际案例研究,并从以下研究中汲取了灵感。
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引用次数: 0
IUENNA – openIng the soUthErn jauNtal as a micro-regioN for future Archaeology: A «para-description» 伊恩纳--开放作为未来考古学微观区域的土伦丘陵:准描述
Pub Date : 2023-12-14 DOI: 10.24072/pcjournal.338
Dominik Hagmann, Franziska Reiner
The Go!Digital 3.0 project IUENNA—an acronym for “open I ng the so U th E rn jau N tal as a micro-regio N for future A rchaeology”—adopts a comprehensive open science methodology and concentrates on the archaeological micro-region of the Jauntal/Podjuna Valley in Carinthia, Austria. Key case studies include the Hemmaberg/gora svete Heme site and its associated locations: Globasnitz/Globasnica/Iuenna, Jaunstein/Podjuna, and St. Ste-fan/Šteben. The Hemmaberg site is among the most extensively researched Late Antique hilltop settlements—dating from the 4 th to the 6 th century AD—and serves as a primary research focus. This site moreover functions as a crucial reference point for early Christianity in the Southeast Alpine region and boasts a rich array of features
Go! Digital 3.0 项目 IUENNA--"open I ng the so U th E rn jau N tal as a micro-regio N for future A rchaeology "的首字母缩写--采用了一种全面的开放科学方法,集中研究奥地利克恩顿州 Jauntal/Podjuna 山谷的考古微型区域。主要案例研究包括 Hemmaberg/gora svete Heme 遗址及其相关地点:Globasnitz/Globasnica/Iuenna, Jaunstein/Podjuna 和 St.海马伯格遗址是研究最为广泛的晚期古代山顶定居点之一,其历史可追溯到公元 4 世纪至 6 世纪,也是主要的研究重点。此外,该遗址还是东南阿尔卑斯山地区早期基督教的重要参照点,并拥有丰富的特征
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引用次数: 0
Does sociality affect evolutionary speed? 社会性会影响进化速度吗?
Pub Date : 2023-12-11 DOI: 10.24072/pcjournal.352
Lluís Socias-Martínez, L. Peckre
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引用次数: 1
Transgenerational cues about local mate competition affect offspring sex ratios in the spider mite Tetranychus urticae 本地配偶竞争的跨代线索影响了荨麻叶螨后代的性别比例
Pub Date : 2023-11-13 DOI: 10.24072/pcjournal.343
Alison B. Duncan, Cassandra Marinosci, Céline Devaux, Sophie Lefèvre, Sara Magalhães, Joanne Griffin, Adeline Valente, Ophélie Ronce, Isabelle Olivieri
In structured populations, competition for mates between closely related males, termed Local Mate Competition (LMC), is expected to select for female-biased offspring sex ratios. However, the cues underlying sex allocation decisions remain poorly studied. Here, we test for several cues in the spider mite Tetranychus urticae, a species that was previously found to adjust the sex ratio of its offspring in response to the number of females within the local population, i.e. a patch. We here investigate whether the offspring sex ratio of T. urticae females changes in response to 1) the current number of females in the same patch, 2) the number of females in the patches of their mothers and 3) their relatedness to their mate. Single females on patches produced similar sex ratios to those of groups of 15 females; their mothers had been in identical conditions of panmixia. The offspring sex ratios of females mated with their brother did not differ from those of females mated with an unrelated male. Females however produced a more female-biased offspring sex ratio if their mothers were alone on a patch compared to 15 other females. Thus, maternal environment is used as a cue for the sex allocation of daughters. We discuss the conditions under which the maternal environment may be a reliable predictor of LMC experienced by grand-sons.
在结构种群中,亲缘关系密切的雄性之间的配偶竞争,称为本地配偶竞争(LMC),预计会选择偏向雌性的后代性别比例。然而,性别分配决定背后的线索仍未得到充分研究。在这里,我们测试了几种线索在叶螨荨麻疹,一个物种,以前被发现调整其后代的性别比例,以响应当地种群中雌性的数量,即一个补丁。本研究探讨了不同斑块中不同雌虫的数量、不同母虫的数量以及不同雌虫与配偶的亲缘关系是否会改变斑胸蚜雌虫后代的性别比例。单雌斑蝶的性别比与15雌斑蝶的性别比相似;它们的母亲也处于同样的panxia状态。与其兄弟交配的雌性后代的性别比例与与非亲属交配的雌性后代的性别比例没有差异。然而,与其他15只雌性相比,如果它们的母亲单独生活在一块土地上,雌性会产生更偏向于雌性的后代性别比例。因此,母性环境被用作女儿性别分配的线索。我们讨论了在哪些条件下,母亲环境可能是孙子经历LMC的可靠预测因子。
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引用次数: 0
Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity 津巴布韦不同蝙蝠物种中星状病毒感染的纵向调查:高遗传星状病毒多样性的证据
Pub Date : 2023-11-08 DOI: 10.24072/pcjournal.340
Vimbiso Chidoti, Hélène De Nys, Malika Abdi, Getrude Mashura, Valérie Pinarello, Ngoni Chiweshe, Gift Matope, Laure Guerrini, Davies Pfukenyi, Julien Cappelle, Ellen Mwandiringana, Dorothée Missé, Elizabeth Gori, Mathieu Bourgarel, Florian Liégeois
Astroviruses (AstVs) have been discovered in over 80 animal species including diverse bat species and avian species. A study on Astrovirus circulation and diversity in different insectivorous and frugivorous chiropteran species roosting in trees, caves and building basements was carried out at 11 different sites across Zimbabwe. Pooled and individual faecal sampling methods were used for this study, with collection dates ranging from June 2016 to July 2021. In two sites, Magweto and Chirundu, sampling was carried out at monthly intervals from August 2020 to July 2021. Astroviruses and bat mitochondrial genes were amplified using pan-AstVs and CytB /12S RNA PCR systems respectively. Phylogenetic analysis of the RdRp Astrovirus sequences revealed a high genetic diversity of astroviruses. All the bat astroviruses tested in this study clustered with the Mamastrovirus genus. Two distinct groups of amplified sequences were identified. One group was comprised of sequences isolated from Hipposideros, Rhinolophus and Eidolon helvum spp. clustered with Human Astrovirus strains: HuAstV types1-6, HuAstV-MLB1-3 and HuAstV-VA-1. The second group comprising the majority of the sequences clustered with different strains of Bat AstVs. Results from the longitudinal study at Magweto and Chirundu showed an overall AstV prevalence of 13.7% and 10.4% respectively. Peaks of AstV infection at Chirundu coincided with the period when juveniles are 4-6 months old. By combining data from our previous work on Coronaviruses, we also anaylzed co-infection of bats with Coronaviruses and Astroviruses at Magweto and Chirundu sites for which the prevalence of co-infection was 2.6% and 3.5% respectively.
星状病毒(AstVs)已在包括多种蝙蝠和鸟类在内的80多种动物中被发现。在津巴布韦11个不同地点对栖息在树木、洞穴和建筑物地下室的不同食虫和食果翼目动物的星状病毒传播和多样性进行了研究。本研究采用了混合和个体粪便采样方法,收集日期为2016年6月至2021年7月。在Magweto和Chirundu两个地点,从2020年8月至2021年7月每月进行一次抽样。采用pan- astv和CytB /12S RNA PCR系统分别扩增星状病毒和蝙蝠线粒体基因。对RdRp星状病毒序列的系统发育分析表明,星状病毒具有较高的遗传多样性。本研究中测试的所有蝙蝠星状病毒都属于星状病毒属。鉴定出两组不同的扩增序列。其中一组由从希波sideros、Rhinolophus和Eidolon helvum spp中分离的序列组成,与人类星状病毒株HuAstV type 1-6、HuAstV- mlb1 -3和HuAstV- va -1聚集。第二组包含大多数序列聚集在不同的蝙蝠astv株上。Magweto和Chirundu的纵向研究结果显示,asv的总体患病率分别为13.7%和10.4%。奇伦都的亚洲猪瘟病毒感染高峰恰好发生在雏鸡4-6个月大的时期。结合之前冠状病毒研究的数据,我们还分析了Magweto和Chirundu站点蝙蝠与冠状病毒和astrovirus的共同感染情况,其中共同感染的患病率分别为2.6%和3.5%。
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引用次数: 0
Assessing species interactions using integrated predator-prey models 利用综合捕食-猎物模型评估物种相互作用
Pub Date : 2023-11-08 DOI: 10.24072/pcjournal.337
Matthieu Paquet, Frédéric Barraquand
Inferring the strength of species interactions from demographic data is a challenging task. The Integrated Population Modelling (IPM) approach, bringing together population counts, capture-recapture, and individual-level fecundity data into a unified model framework, has been extended from single species to the community level. This allows to specify IPMs for multiple species with interactions specified as links between vital rates and stage-specific densities. However, there is no evaluation of such models when interactions are actually absent---while any interaction inference method runs the risk of producing false positives. We investigate here whether multispecies IPMs could output interactions where there are in fact none, building on an existing predator-prey IPM. We show that interspecific density-dependence estimates are centered on zero when simulated to be zero, and therefore their estimation is unbiased. Their coverage probability, quantifying how many times credible intervals include zero, is also satisfactory. We further confirm that adding random temporal variation to multispecies density-dependent link functions does not alter these results. This study therefore reaffirms the potential of multispecies IPMs to infer correctly how biotic interactions influence demography, although future studies should investigate model misspecifications.
从人口统计数据推断物种相互作用的强度是一项具有挑战性的任务。综合种群模型(IPM)方法将种群数量、捕获-再捕获和个体水平的繁殖力数据整合到一个统一的模型框架中,已从单一物种扩展到群落水平。这允许为多个物种指定ipm,并将相互作用指定为生命速率和阶段特定密度之间的联系。然而,当相互作用实际上不存在时,就没有对这些模型进行评估——而任何相互作用推理方法都有产生误报的风险。我们在此研究多物种IPM是否可以在实际上没有相互作用的情况下输出相互作用,建立在现有的捕食者-猎物IPM的基础上。我们表明,当模拟为零时,种间密度依赖估计以零为中心,因此它们的估计是无偏的。它们的覆盖概率(量化可信区间包含零的次数)也令人满意。我们进一步证实,将随机时间变化添加到多物种密度相关的连接函数中不会改变这些结果。因此,这项研究重申了多物种ipm在正确推断生物相互作用如何影响人口统计学方面的潜力,尽管未来的研究应该调查模型的错误规范。
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引用次数: 0
Telomere length varies with sex, hatching rank and year of birth in the Little Owl, Athene noctua 端粒长度随性别、孵化等级和出生年份的不同而变化
Pub Date : 2023-11-08 DOI: 10.24072/pcjournal.341
François Criscuolo, Inès Fache, Bertrand Scaar, Sandrine Zahn, Josefa Bleu
Telomeres are non-coding DNA sequences located at the end of linear chromosomes, protecting genome integrity. In numerous taxa, telomeres shorten with age and telomere length (TL) is positively correlated with longevity. Moreover, TL is also affected by environmental stressors and/or resource-demanding situations particularly during early-life. Thus, TL has been used as a physiological marker of individual quality and also as an indicator of population trend in conservation physiology. In this study, we investigated the effects of hatching rank, year of birth (2014 to 2017), sex and nest environment on TL of 137 Little Owls nestlings (Athene noctua). Little Owls’ populations in Europe showed a marked declined in the end of the 20th century. Nowadays, in the studied Alsatian population, the population is increasing. In this study, our results indicated that telomeres are longer in females and, independently of sex, in nestlings with the highest body condition. There was also a negative effect of hatching rank but only for last-hatched nestlings in large clutches of 5 nestlings. We did not find any effect of the environmental covariates on nestlings’ TL. Finally, we found that nestlings’ TL were shorter the last year of the study, while nestlings’ body condition stayed unchanged over the same period. This result is intriguing given the local positive population dynamics and is further discussed in the context of physiological conservation. Future studies should investigate the link between reduced TL and survival prospects in this species.
端粒是位于线性染色体末端的非编码DNA序列,保护基因组的完整性。在许多分类群中,端粒随着年龄的增长而缩短,端粒长度与寿命呈正相关。此外,长期学习还受到环境压力因素和/或资源需求情况的影响,特别是在生命早期。因此,在保护生理学中,TL不仅可以作为个体品质的生理标记,也可以作为种群趋势的指示物。本研究研究了137只小猫头鹰(Athene noctua)雏鸟的孵化等级、出生年份(2014 ~ 2017年)、性别和巢环境对TL的影响。20世纪末,欧洲小猫头鹰的数量明显下降。如今,在研究的阿尔萨斯人口中,人口正在增加。在这项研究中,我们的结果表明,端粒在雌性中更长,并且与性别无关,在身体状况最高的雏鸟中更长。孵化等级也有负影响,但仅对5只大窝中最后孵出的雏鸟有负影响。我们没有发现环境协变量对雏鸟的TL有任何影响。最后,我们发现在研究的最后一年,雏鸟的TL变短了,而同期雏鸟的身体状况保持不变。考虑到当地积极的种群动态,这一结果是有趣的,并在生理保护的背景下进一步讨论。未来的研究应进一步探讨TL减少与该物种生存前景之间的关系。
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引用次数: 0
Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis 用化合物特异性同位素分析鉴定砂质和泥质潮间带沉积物中微生物外聚合物生成物
Pub Date : 2023-10-31 DOI: 10.24072/pcjournal.336
Cédric Hubas, Julie Gaubert-Boussarie, An-Sofie D’Hondt, Bruno Jesus, Dominique Lamy, Vona Meleder, Antoine Prins, Philippe Rosa, Willem Stock, Koen Sabbe
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引用次数: 0
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