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High quality genome assembly and annotation (v1) of the eukaryotic terrestrial microalga Coccomyxa viridis SAG 216-4 真核陆生微型藻类 Coccomyxa viridis SAG 216-4 的高质量基因组组装和注释 (v1)
Pub Date : 2024-08-08 DOI: 10.24072/pcjournal.447
Anton Kraege, Edgar A. Chavarro-Carrero, Nadège Guiglielmoni, Eva Schnell, Joseph Kirangwa, Stefanie Heilmann-Heimbach, Kerstin Becker, Karl Köhrer, Philipp H. Schiffer, Bart P. H. J. Thomma, Hanna Rovenich
Unicellular green algae of the genus Coccomyxa are recognized for their worldwide distribution and
椰菜属(Coccomyxa)单细胞绿藻因其分布于世界各地和
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引用次数: 0
Ecotoxicity of lanthanides to Daphnia magna: insights from elemental behavior and speciation in a standardized test medium 镧系元素对大型蚤的生态毒性:从标准化测试介质中的元素行为和种类中获得的启示
Pub Date : 2024-07-19 DOI: 10.24072/pcjournal.440
D. Vignati, Loïc A Martin, L. Poirier, A. Zalouk‐Vergnoux, C. Fouque, Clément Bojic, Christophe Hissler, Carole Cossu-Leguille
Lanthanides (LNs) are a group of 15 elements with steadily increasing economical importance due to their multiple uses in technologies essential for sustainable ecological, digital and energetic transitions. Although knowledge on LN ecotoxicology has greatly improved over the last decade, uncertainty persists with regard to their actual hazard and risk in freshwater environments. In particular, only limited information is available on i) the actual relationships between LN speciation vs . ecotoxicological responses in standardized laboratory tests and ii) the existence of regular and predictable patterns in LN ecotoxicity (expressed as e.g. , EC 50 ) along the LN series. The present paper provides the first report on the ecotoxicity of all lanthanides (except Pm) for the freshwater crustacean Daphnia magna along with an unprecedented level of detail on LN speciation in the exposure medium. Experimental data show that exposure concentrations can decrease by up to 95 % over the test duration, with the percentage decrease being inversely related with LN atomic mass. Thermodynamic speciation calculations confirm the possible formation of insoluble species, mainly LN carbonates. However, the corresponding theoretical solubility limits do not fully agree with measured concentrations at the end of the tests. Experimental verification of exposure concentrations (as a minimum at the beginning and end of laboratory tests) remains therefore mandatory to reach proper conclusions as to the ecotoxicity of each LN. A decreasing trend in ecotoxicity can actually be observed along the LN series when temporal changes in the exposure concentrations are properly accounted for. However, this trend remains dependent on exposure time and selected exposure metrics. This and other caveats must be considered in future research to reach a community-based consensus for the proper hazard and risk assessment of LN towards daphnids and other aquatic organisms.
镧系元素(LNs)由 15 种元素组成,由于其在可持续生态、数字和能源转型所必需的技术中的多种用途,其经济重要性稳步上升。尽管过去十年中有关镧系元素生态毒理学的知识有了很大的提高,但其在淡水环境中的实际危害和风险仍然存在不确定性。尤其是在以下方面,目前只有有限的信息:i) 在标准化实验室测试中,LN 种类与生态毒理学反应之间的实际关系;ii) LN 生态毒性(以 EC 50 表示)在 LN 系列中是否存在规律性和可预测的模式。本文首次报告了所有镧系元素(铂除外)对淡水甲壳动物大型蚤的生态毒性,并前所未有地详细介绍了接触介质中镧系元素的种类。实验数据表明,在试验持续时间内,暴露浓度最多可降低 95%,降低的百分比与镧系元素的原子质量成反比。热力学标示计算证实可能会形成不溶性物质,主要是 LN 碳酸盐。然而,相应的理论溶解度极限与试验结束时的测量浓度并不完全一致。因此,为了对每种 LN 的生态毒性得出正确的结论,必须对接触浓度(至少在实验室测试开始和结束时)进行实验验证。如果适当考虑接触浓度的时间变化,实际上可以观察到 LN 系列的生态毒性呈下降趋势。不过,这种趋势仍然取决于暴露时间和选定的暴露指标。在今后的研究中,必须考虑到这一点和其他注意事项,以便达成基于社区的共识,对水蚤和其他水生生物进行适当的 LN 危害和风险评估。
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引用次数: 0
T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses T7 DNA 聚合酶处理可提高双链和单链 DNA 病毒的定量测序能力
Pub Date : 2024-07-11 DOI: 10.24072/pcjournal.437
Maud Billaud, Ilias Theodorou, Quentin Lamy-Besnier, Shiraz A. Shah, François Lecointe, Luisa De Sordi, Marianne De Paepe, Marie-Agnès Petit
Bulk microbiome, as well as virome-enriched shotgun sequencing only reveals the double-stranded DNA (dsDNA) content of a given sample, unless specific treatments are applied. However, genomes of viruses often consist of a circular single-stranded DNA (ssDNA) molecule. Pre-treatment and amplification of DNA using the multiple displacement amplifica-tion (MDA) method enables conversion of ssDNA to dsDNA, but this process can lead to over-representation of these circular ssDNA genomes. A more recent alternative permits to bypass the amplification
大量微生物组和富集病毒组的霰弹枪测序只能揭示特定样本中的双链 DNA(dsDNA)含量,除非进行特殊处理。然而,病毒基因组通常由环状单链 DNA(ssDNA)分子组成。使用多重位移扩增(MDA)法对 DNA 进行预处理和扩增,可将 ssDNA 转化为 dsDNA,但这一过程会导致这些环状 ssDNA 基因组的过度呈现。最新的替代方法允许绕过扩增
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引用次数: 0
Differences in specificity, development time and virulence between two acanthocephalan parasites, infecting two cryptic species of Gammarus fossarum 两种棘头蚴寄生虫在特异性、发育时间和毒力方面的差异,它们感染了两种隐蔽的福寿螺
Pub Date : 2024-07-08 DOI: 10.24072/pcjournal.434
Alexandre Bauer, Lucie Develay Nguyen, S. Motreuil, Maria Teixeira, Nelly Debrosse, Thierry Rigaud
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引用次数: 0
Multiproxy analysis exploring patterns of diet and disease in dental calculus and skeletal remains from a 19th century Dutch population 从 19 世纪荷兰人的牙结石和骨骼遗骸中探索饮食和疾病模式的多代理分析
Pub Date : 2024-04-23 DOI: 10.24072/pcjournal.414
B. P. Bartholdy, Jørgen B. Hasselstrøm, Lambert K. Sørensen, Maia Casna, Historisch Menno Hoogland, Genootschap Beemster, Amanda G. Henry, Historisch Genootschap Beemster
Dental calculus is an excellent source of information on the dietary patterns of past populations, including consumption of plant-based items. The detection of plant-derived residues such as alkaloids and their metabolites in dental calculus provides direct evidence of consumption by individuals within a population. We conducted a study on 41 individuals from Middenbeemster, a 19th century rural Dutch archaeological site. Skeletal and dental analysis was performed to explore potential relationships between pathological lesions and presence of alkaloids. Dental calculus was analysed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-ESI-MS/MS). We were able to detect nicotine, cotinine, caffeine, theophylline, and salicylic acid, suggesting the consumption of tea and coffee and smoking of tobacco on an individual scale, which is also confirmed by historic documentation and identification of pipe notches in the dentition. Nicotine and/or cotinine was present in 56% of individuals with at least one visible pipe notch. There is some influence of skeletal preservation on the detection of alkaloids, with higher quantities of compounds extracted from well-preserved individuals, and we observe a positive relationship between weight of the calculus sample and quantity of detected compounds, as well as between chronic maxillary sinusitis and the presence of multiple alkaloids. There are many limitations that will need to be addressed going forward with this type of analysis; we stress the need for more systematic research on the consumption of alkaloid-containing items and their subsequent concentration and preservation in dental calculus, in addition to how mode of consumption may affect concentrations in the dentition. Despite the limitations, this preliminary study illustrates many benefits of using calculus to target a variety of compounds that could have been consumed as medicine or diet. This method allows us to directly address specific individuals, which can be especially useful in individuals that are not always well-documented in historic documentation, such as rural populations, and especially children and women.
牙结石是了解过去人群饮食模式(包括植物性食物消费)的绝佳信息来源。牙结石中生物碱及其代谢物等植物残留物的检测,可直接证明人群中个体的消费情况。我们对来自 19 世纪荷兰农村考古遗址 Middenbeemster 的 41 人进行了研究。我们对骸骨和牙齿进行了分析,以探讨病变与生物碱存在之间的潜在关系。牙结石采用超高效液相色谱-串联质谱法(UHPLC-ESI-MS/MS)进行分析。我们能够检测到尼古丁、可替宁、茶碱、茶碱和水杨酸,这表明在个人范围内饮用茶和可乐以及吸烟,历史文献和牙齿上烟斗缺口的鉴定也证实了这一点。在至少有一个可见烟斗缺口的个体中,56%存在尼古丁和/或可替宁。骨骼的保存对生物碱的检测有一定的影响,保存完好的个体提取的化合物数量较多,我们观察到牙结石样本的重量与检测到的化合物数量之间存在正相关关系,慢性上颌窦炎与多种生物碱的存在之间也存在正相关关系。这种分析方法还有许多局限性需要解决;我们强调,除了研究食用方式如何影响牙结石中生物碱的浓度外,还需要对食用含生物碱的食物及其在牙结石中的浓度和保存情况进行更系统的研究。尽管存在局限性,但这项初步研究说明了利用牙结石来研究可能作为药物或饮食食用的各种化合物的诸多益处。这种方法使我们能够直接针对特定的个体进行研究,这对于那些在历史文献中并不总是有详细记载的个体尤其有用,例如农村人口,特别是儿童和妇女。
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引用次数: 1
Transcriptomic responses of sponge holobionts to in situ, seasonal anoxia and hypoxia 海绵全缘体对原位、季节性缺氧和缺氧的转录组反应
Pub Date : 2024-04-15 DOI: 10.24072/pcjournal.410
Brian W. Strehlow, Astrid Schuster, Warren R. Francis, Lisa K Eckford-Soper, Beate Kraft, Rob McAllen, R. Nielsen, S. Mandrup, Donald E. Canfield
Deoxygenation can be fatal for many marine animals; however, some sponge species are tolerant of hypoxia and anoxia. Indeed, two sponge species, Eurypon sp. 2 and Hymeraphia stellif-era, survive seasonal anoxia for months at a time. To understand their tolerance mechanisms, we performed differential gene expression analyses on the sponges, their mitochondria and their microbial symbionts under in situ conditions of normoxia, hypoxia and anoxia. Each species
对许多海洋动物来说,缺氧可能是致命的;然而,有些海绵物种却能耐受缺氧和缺氧。事实上,有两个海绵物种--Eurypon sp.为了了解它们的耐受机制,我们在常氧、缺氧和缺氧的原位条件下对海绵、它们的线粒体及其微生物共生体进行了不同的基因表达分析。每个物种
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引用次数: 0
Social Network Analysis of Ancient Japanese Obsidian Artifacts Reflecting Sampling Bias Reduction 反映取样偏差减少情况的日本古代黑曜石文物社交网络分析
Pub Date : 2024-04-08 DOI: 10.24072/pcjournal.409
Fumihiro Sakahira, Hiro'omi Tsumura
This study aims to investigate the dynamics of obsidian trade networks during the Jomon period (15,000–2,400 years cal BP), the hunting and gathering era in Japan. To improve regional representation and reduce the distortions caused by small sample sizes, we performed clustering based on a large-scale dataset and conducted social network analysis. The research results revealed that the trade networks during the Jomon period were not constant; they expanded throughout the southern Kanto region during the Middle Jomon period (5,500–4,500 years cal BP) and ceased to function during the Late Jomon period (4,500–3,200 years cal BP). Furthermore, to enhance the readability and inter-pretability of the dataset, we implemented clustering using the density-based spatial clustering of applications with noise (DBSCAN) method. The results showed that in every time division of the Jomon period, the mean intra-cluster cosine similarity of each cluster was higher than the similarity between sites outside the clusters
本研究旨在调查日本狩猎和采集时代绳文时代(公元前 15000-2400 年)黑曜石贸易网络的动态。为了提高区域代表性,减少小样本量造成的失真,我们在大规模数据集的基础上进行了聚类,并进行了社会网络分析。研究结果表明,绳文时代的贸易网络并不是一成不变的;在绳文中期(5500-4500 年(公元前)),贸易网络扩展到整个关东南部地区;在绳文晚期(4500-3200 年(公元前)),贸易网络停止运作。此外,为了提高数据集的可读性和相互可读性,我们使用基于密度的带噪声应用空间聚类(DBSCAN)方法对数据集进行了聚类。结果表明,在绳文时代的每个时间分段中,每个聚类的聚类内余弦相似度平均值均高于聚类外遗址之间的相似度
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引用次数: 0
Genetic background of body reserves in laying hens through backfat thickness phenotyping 通过背膘厚度表型分析蛋鸡体储的遗传背景
Pub Date : 2024-04-04 DOI: 10.24072/pcjournal.412
N. Bedere, Joëlle Dupont, Y. Baumard, Christophe Staub, D. Gourichon, Elleboudt Frédéric, Pascale Le Roy, Tatiana Zerjal
In this study, we pursued three primary objectives: firstly to test and validate the pheno-typing of backfat thickness as an indicator of the overall fatness of laying hens; secondly, to estimate genetic parameters for this trait; thirdly, to study the phenotypic and genetic relationships between this trait and other traits related to production and body composition. To address these questions, hens from two lines under divergent selection for residual feed intake, were phenotyped for body weight, body composition traits (backfat, total fat volume, and blood adipokines levels), and egg number. Linear mixed models enabled to estimate variance components and calculate
在这项研究中,我们有三个主要目标:首先,测试和验证背膘厚度作为蛋鸡整体肥胖指标的表型;其次,估计该性状的遗传参数;第三,研究该性状与其他与生产和身体组成相关的性状之间的表型和遗传关系。为了解决这些问题,对两个品系的母鸡进行了体重、体成分性状(背脂、总脂肪量和血液脂肪因子水平)和蛋数的表型分析。线性混合模型可以估计方差成分并计算
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引用次数: 0
Dealing with post-excavation data: the Omeka S TiMMA web-database 处理发掘后数据:Omeka S TiMMA 网络数据库
Pub Date : 2024-01-24 DOI: 10.24072/pcjournal.366
Bastien Rueff
This paper reports on the creation and use of a web database designed as part of the TiMMA project with the Content Management System Omeka S. Rather than resulting in a technical manual, its goal is to analyze the relevance of using Omeka S in the frame of a post-excavation project, gathering a wide team of researchers from several countries, working in their own language and having their own specialty. Designed to assist organizations and institutions in creating and managing digital collections, Omeka S offers a number of commodities from the perspective of both the administrator(s) and the users, the most significant being its efficiency in capitalizing on linked data standards for items description. Additionally, one of the successful achievements of this platform is to benefit the open-science tools more broadly, permitting for instance, as was done in this project, to import images with the iiiF API and bibliographical references with the Zotero module, thus avoiding the creation of new digital files and metadata. Designed with simplicity in mind, the graphic interface of Omeka S makes it a particularly appropriate tool for
本文介绍了在 TiMMA 项目中使用内容管理系统 Omeka S 创建和使用网络数据库的情况。本文的目的不是编写技术手册,而是分析在发掘后项目框架内使用 Omeka S 的相关性,该项目汇集了来自多个国家的研究人员,他们使用各自的语言和专业开展工作。Omeka S 的设计目的是帮助组织和机构创建和管理数字藏品,从管理员和用户的角度来看,它提供了许多商品,其中最重要的是它在利用链接数据标准进行物品描述方面的便利性。此外,该平台的成功之处还在于为开放科学工具提供了更广泛的帮助,例如,在本项目中,可以使用 iiiF API 导入图像,使用 Zotero 模块导入参考文献,从而避免创建新的数字文件和元数据。Omeka S 的图形界面以简洁为设计理念,使其成为特别适合以下用途的工具
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引用次数: 0
Mark loss can strongly bias estimates of demographic rates in multi-state models: a case study with simulated and empirical datasets 标记丢失会对多州模型中的人口统计率估算造成严重偏差:利用模拟数据集和经验数据集进行的案例研究
Pub Date : 2023-12-21 DOI: 10.24072/pcjournal.348
F. Touzalin, Eric J. Petit, Emmanuelle Cam, Claire Stagier, E. Teeling, S. Puechmaille
1. The development of methods for individual identification in wild species and the refinement of Capture-Mark-Recapture (CMR) models over the past few decades have greatly improved the assessment of population demographic rates to address ecological and conservation questions. In particular, multi-state models, which offer flexibility in analysing complex study systems, have gained popularity within the ecological community. In this study, we focus on the issue of mark loss and the associated recycling of remarked individuals, which requires further exploration given the increasing use of these models. 2. To fill this knowledge gap, we employed a wide range of simulation scenarios that reflect commonly encountered real case studies, drawing inspiration from the
1.过去几十年来,野生物种个体识别方法的发展和捕获-标记-再捕获(CMR)模型的改进极大地改善了对种群人口率的评估,从而解决了生态和保护问题。尤其是多态模型,它在分析复杂的研究系统方面具有很强的灵活性,在生态学界越来越受欢迎。在本研究中,我们将重点关注标记丢失问题以及与之相关的已注释个体的回收问题。2.为了填补这一知识空白,我们采用了多种模拟情景,这些情景反映了常见的实际案例研究,并从以下研究中汲取了灵感。
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引用次数: 0
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