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Evaluation of rapid beta-lactam antibiotic residues detection kits for raw milk 原料奶中β -内酰胺类抗生素残留快速检测试剂盒的评价
Pub Date : 2021-11-30 DOI: 10.5897/ajfs2021.2145
W. N. Teresiah, Omwamba Mary, S. Patrick
Platform tests are usually simple and rapid quality control tests which serve as a basis for accepting, setting aside or rejecting raw milk. Some of the rapid antibiotic residues test kits available in the Kenyan market include: Delvo test Fast BL, Delvo Sulphadiazine Penicillin No Tablet (SPNT), Mtusbio Betalactam BLQ Rapid Test Kit and Ringbio beta-lactam, tetracycline, sulfa drugs, BTS 3 in 1 TriTest S. Ndungu Antibiotic residues (NAR) is a colour comparison test that is simple, rapid and does not require electric power in its analysis procedure. This study compared these four rapid antibiotics tests with novel NAR test method in terms of sensitivity and specificity. A total of 28 samples were prepared; 8 milk samples without residues were spiked with milk known to have beta-lactam residues while 10 had no residues and 10 had known beta-lactam (benzyl penicillin) residues. The four analysis methods were carried out as described in their technical bulletins and manuals. For the NAR test, 50 mg of its active ingredient was mixed with 3 ml of milk and colour change observations made. The sensitivity and specificity of the NAR test was found to be 66.7 and 100% respectively while for all the other four tests, sensitivity and specificity was established to be 100% for each. The Kappa coefficient was 0.5882 which indicates moderate agreement, between NAR test method and the other test methods, according to Landis-Koch scale. The odds ratio exhibited positive association between the NAR test and the four methods. NAR test is best applicable at the milk collection routes or farm level before bulking for transportation.
平台测试通常是简单而快速的质量控制测试,作为接受、搁置或拒绝原料奶的基础。肯尼亚市场上可获得的一些抗生素残留快速检测试剂盒包括:Delvo test Fast BL、Delvo Sulphadiazine Penicillin No片剂(SPNT)、Mtusbio Betalactam BLQ快速检测试剂盒和Ringbio β -内酰胺、四环素、磺胺类药物BTS 3合一TriTest S. Ndungu抗生素残留(NAR)是一种简单、快速且在分析过程中不需要电力的颜色比较试验。本研究将这四种抗生素快速检测方法与新型NAR检测方法在敏感性和特异性方面进行比较。共制备样品28份;8份没有残留的牛奶样品中加入了已知有-内酰胺残留的牛奶,10份没有残留,10份已知有-内酰胺(苄青霉素)残留。这四种分析方法是按照它们的技术公报和手册中的描述进行的。在NAR测试中,将50毫克活性成分与3毫升牛奶混合,观察颜色变化。NAR检测的敏感性和特异性分别为66.7和100%,而其他四项检测的敏感性和特异性均为100%。根据Landis-Koch量表,NAR检验方法与其他检验方法的Kappa系数为0.5882,一致性中等。四种方法与NAR检验的比值比均呈显著正相关。NAR测试最适用于牛奶收集路线或在运输前的农场水平。
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引用次数: 0
A novel platform test to detect beta-lactam residues in raw milk 一种检测原料奶中β -内酰胺残留的新型平台试验
Pub Date : 2021-10-31 DOI: 10.5897/ajfs2021.2135
Teresiah W. Ndungu, P. Muliro, M. Omwamba
The mastitis causing microorganisms resist beta-lactam antibiotics by releasing beta-lactamase and the enzyme can be traced in raw milk. This study was aimed at developing a novel platform test to detect beta-lactam antibiotics residues in raw milk based on the Hardy Diagnostic Beta-lactamase Test (HDBT) reagent. The HDBT ingredients modified were penicillin, sodium chloride, trisodium citric acid, trisodium phosphate and phenol red dissolved in distilled water. Pooled raw milk samples were obtained from 3 Friesians and 3 Ayrshires lactating cows identified to have subclinical mastitis and treated using beta-lactam antibiotics. The appropriate mixing ratios were investigated at nine levels. Investigation on the effect of breeds on the test method results was also carried out. Evaluations to determine the colour differences between beta-lactam positive and negative raw milk samples for all the experiments were carried out using trained panelists. The results indicate that gradual addition of trisodium phosphate and phenol red in the reagent showed significant difference (P ≤ 0.05) between a beta-lactam positive and negative raw milk sample. Ratio 5:5 was selected as the best and had significant difference (P ≤ 0.05) from the others. Conversely, the test method indicated no significant difference (P ≤ 0.05) between the Friesians and Ayrshires raw milk samples. This method can be used along the raw milk collection routes to accept, set aside or reject raw milk suspected to have residues. The colour observed for a beta-lactam negative sample was fuchsia purple while peach or pink signified a positive sample.
乳腺炎微生物通过释放β -内酰胺酶来抵抗β -内酰胺类抗生素,而这种酶可以在原料奶中找到。本研究旨在建立一种基于Hardy诊断β -内酰胺酶试验(HDBT)试剂检测原料奶中β -内酰胺类抗生素残留的新型检测平台。改性的HDBT成分为青霉素、氯化钠、柠檬酸三钠、磷酸三钠和蒸馏水溶解酚红。收集了3头弗里斯兰奶牛和3头艾尔郡奶牛的原料奶样本,这些奶牛被确定患有亚临床乳腺炎,并使用β -内酰胺类抗生素治疗。在9个水平上考察了合适的混合比例。研究了品种对试验方法结果的影响。所有实验中β -内酰胺阳性和阴性原料奶样品的颜色差异评估均由训练有素的小组成员进行。结果表明,在试剂中逐渐添加磷酸三钠和酚红对β -内酰胺阳性和阴性原料乳样品有显著差异(P≤0.05)。选择5:5的比例为最佳,与其他比例差异显著(P≤0.05)。相反,弗里斯兰和艾尔郡原料奶样品间的差异不显著(P≤0.05)。该方法可用于沿原奶收集路线接受、搁置或拒绝怀疑有残留的原奶。β -内酰胺阴性样品的颜色为紫红色,阳性样品为桃红色或粉红色。
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引用次数: 1
Uses and microbiological quality of fresh cows milk sold in three markets in South Benin 南贝宁三个市场销售的鲜奶的用途和微生物质量
Pub Date : 2021-10-31 DOI: 10.5897/ajfs2021.2134
Djobo Oumarou, Sina Haziz, Damè Kombienou Pocoun, Morenikè Djenontin Wilfrande, Ahyi Virgile, Y. Issaka, A. Adolphe, Rendueles Manuel, B. Lamine
1 Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Sciences and Technology, University of Abomey-Calavi, Benin. 2 National Agronomic Research Institute of Benin, 01 BP 884 Cotonou, Republic of Benin. 3 Department of Chemical Engineering, IRGIB-Africa, Benin. 4 Animal Biotechnology and Meat Technologies Laboratory, Animal production Department, EPAC, University of Abomey-Calavi, Benin. 5 Department of Chemical and Environmental Engineering, Polytechnic School of Engineering, Gijón Campus, University of Oviedo, Spain.
1贝宁阿贝美-卡拉维大学科学技术学院生物化学与细胞生物学系微生物生物学与分子分型实验室。2贝宁国家农学研究所,贝宁共和国科托努01 BP 884 . 3贝宁生物技术研究所-非洲化学工程系。4阿贝美-卡拉维大学生物技术与肉类技术实验室,动物生产部,EPAC,动物生物技术与肉类技术实验室,5西班牙奥维耶多大学工程技术学院化学与环境工程系Gijón
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引用次数: 1
Encapsulation and gastrointestinal endurance of Lactobacillus reuteri DSM 17938 strain with emulsion polymerization 乳状聚合法制备罗伊氏乳杆菌DSM 17938的包封性和胃肠耐力
Pub Date : 2021-09-30 DOI: 10.5897/AJFS2021.2110
E. Çelik, Ö. Turgay
L. reuteri DSM 17938 strain was encapsulated using emulsion polymerization technique with alginate and fructooligosaccharide at five different concentrations between 0 and 1.5%. This study aimed to improve the gastrointestinal system (GIS) viability of L. reuteri. Encapsulation yield was calculated and found to be between 98.67 and 86.88%, and SEM imaging was performed for beads, and their sizes were found to range from 68.81 µm to 351.0 µm. In addition, microbial growth in GIS was indicated for 3 h at intervals of one hour. 0.75% fructooligosaccharide plus 2% alginate capsules yielded the highest viability in a simulated gastric environment. At the end of 3 h, these capsules were decreased 0.39±0.03 logarithmic cycle, but the non-encapsulated control sample was decreased 2.10±0.16 log. The control sample was decreased by 5.8 log cycle in the simulated bile environment, but capsules were decreased by 2.5-3.4 log cycle on average. The result was statistically significant and showed that the encapsulation process protected the survival of microorganisms in GIS.   Key words: Encapsulation, gastrointestinal system, lactic acid bacteria, survival rate.
采用海藻酸盐和低聚果糖在0 ~ 1.5%浓度范围内的乳液聚合技术对罗伊氏乳杆菌DSM 17938进行包封。本研究旨在提高罗伊氏乳杆菌胃肠道系统(GIS)的活力。计算包封率为98.67 ~ 86.88%,对微球进行扫描电镜成像,微球尺寸为68.81 ~ 351.0µm。此外,每隔1小时测定GIS中微生物生长3小时。0.75%低聚果糖加2%海藻酸盐胶囊在模拟胃环境中产生最高的生存能力。在3 h结束时,这些胶囊降低了0.39±0.03个对数周期,而未包封的对照样品降低了2.10±0.16个对数周期。在模拟胆汁环境中,对照样品减少了5.8 log cycle,而胶囊平均减少了2.5-3.4 log cycle。结果具有统计学意义,表明包封工艺保护了GIS中微生物的存活。关键词:胶囊化,胃肠系统,乳酸菌,存活率。
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引用次数: 1
Evaluation of chemical composition of Dacryodes edulis (african pear) seed oil at different stages of fruit maturation 非洲梨种子油在果实成熟不同阶段的化学成分评价
Pub Date : 2021-09-30 DOI: 10.5897/AJFS2017.1562
E. Uhunmwangho, E. S. Omoregie
Nutritional and industrial processes have increased the demand for oils and this in turn has led to the search for oils from different types of seeds for possible development and use. It is in this vein that the fruit pulp of Dacryodes edulis was extracted with n-hexane (soxhlet extraction at 65°C). The proximate composition, antinutrient and mineral content of freshly harvested D. edulis fruit pulp from 4 weeks after anthesis (WAA) to fruit maturation were assessed in this study. Data obtained for the  proximate composition at matured stages of fruit development revealed high amount of fat (53.4 ± 1.35%; moisture (47.2 ± 1.20%); crude protein (17.0 ± 0.48%); carbohydrate (21.70  ± 0.98%); low crude fiber (1.60 ± 0.75%) and ash content (8.0 ± 0.81%) which contained higher amount of minerals  such as of Fe (62.71 ± 0.34 ppm), Mn (21.05 ± 0.18 ppm), Cu (10.12 ± 0.17 ppm)  and low in Pb (6.17 ± 0.13 ppm), Ni (1.8 ± 0.56 ppm), Ca (0.41± 0.07%), Cr and Cd were not detected throughout the development stages at 20 WAA as compared to immature stages of 4, 6, 8, 10, 12, 14 and 16 WAA. The level of antinutrient factors are oxalate (5.2 ± 0.91 mg/100 g); phytate (0.32±0.02 mg/100 g) and cyanogenic glycosides (0.14 ± 0.02) which recorded lower content, except tannins (82.11 ± 0.33) mg/100 g which recorded significant (p < 0.05) higher content at 20 WAA when compared with immature stages in 4, 6, 8, 10, 12, 14 and 16 WAA. This study revealed the nutritional profile of the fruit pulp as good sources of plant protein, carbohydrate and fat, with a reduction in the level of some anti-nutrients in matured fruits which are potentials that could be exploited by food and pharmaceutical industries.   Key words: Dacryodes edulis, proximate, antinutrient, n-hexane, anthesis.
营养和工业过程增加了对油的需求,这反过来又导致从不同类型的种子中寻找油以供可能的开发和使用。正是在这个脉络中,用正己烷(65℃索氏萃取)提取了毛豆果肉。本研究对新鲜采收的毛竹果肉在开花后4周至果实成熟期间的近似成分、抗营养物质和矿物质含量进行了测定。在果实发育成熟阶段获得的近似成分数据显示,脂肪含量高(53.4±1.35%);水分(47.2±1.20%);粗蛋白质(17.0±0.48%);碳水化合物(21.70±0.98%);粗纤维含量(1.60±0.75%)和灰分含量(8.0±0.81%)较低,其中铁(62.71±0.34 ppm)、锰(21.05±0.18 ppm)、铜(10.12±0.17 ppm)含量较高,Pb(6.17±0.13 ppm)、Ni(1.8±0.56 ppm)、Ca(0.41±0.07%)、Cr和Cd含量较低,与未成熟的4、6、8、10、12、14和16 WAA相比,在20 WAA发育阶段均未检测到。抗营养因子水平为草酸盐(5.2±0.91 mg/100 g);植酸含量(0.32±0.02 mg/100 g)和氰苷含量(0.14±0.02 mg/100 g)较低,但单宁含量(82.11±0.33)mg/100 g显著高于4、6、8、10、12、14和16 WAA的未成熟阶段(p < 0.05)。该研究揭示了果肉作为植物蛋白、碳水化合物和脂肪的良好来源的营养特征,并降低了成熟果实中某些抗营养素的水平,这是食品和制药工业开发利用的潜力。关键词:毛竹,近物,抗营养,正己烷,开花
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引用次数: 0
Physico-chemical properties of kernel from coconut (Cocos nucifera L.) varieties grown at the Kenyan Coast 肯尼亚海岸种植的椰子(Cocos nucifera L.)品种籽粒的物理化学性质
Pub Date : 2021-08-31 DOI: 10.5897/ajfs2021.2116
O. A. Gaston, N. Daniel, N. Arnold
{"title":"Physico-chemical properties of kernel from coconut (Cocos nucifera L.) varieties grown at the Kenyan Coast","authors":"O. A. Gaston, N. Daniel, N. Arnold","doi":"10.5897/ajfs2021.2116","DOIUrl":"https://doi.org/10.5897/ajfs2021.2116","url":null,"abstract":"","PeriodicalId":7509,"journal":{"name":"African Journal of Food Science","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73041292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of the microbiological and physico-chemical characteristics of local tomato Solanum lycopersicum puree produced on a small scale in Togo 多哥当地小规模生产番茄茄泥的微生物学和理化特性评价
Pub Date : 2021-08-31 DOI: 10.5897/ajfs2019.1862
Gott’liebe Goka Mawuena, Dufrechou Marie, Picouet Pierre, Ameyapoh Yaovi
Tomatoes are produced, processed and widely consumed by the Togolese population. However, the local production is not developed enough to be able to provide locally processed tomatoes. In order to promote local development, stimulate local processing and reduce post-harvest losses, the processing into puree was undertaken with three locally grown varieties of tomatoes in Togo (Aklikonvi, Tohounvi, and Pomvi). A method developed at the LAMICODA laboratory was used and the process was adapted to be mastered by any local producers. Microbiological and physico-chemical analysis of crushed tomatoes and tomato purees of the three different varieties were performed in order to validate product stability and to determine final product physico-chemical and nutritional qualities. Results showed that the hygienic quality of these tested products was validated according to the criteria considered by the European Union. Also, the results indicated that purees produced contained lycopene (3.94 mg to 7.36 mg/100 g), vitamin E (0.38 mg to 1.14 mg/100 g), β-carotene (0.27 mg to 0.56 mg/100 g), and sugars (such as fructose: 0.75 g to 1.56 g/100 g; glucose: 0.78 g to 1.52 g/100 g) whatever the variety. The total sugar content is significantly different (p<0.05) for crushed tomatoes and purees. A deterioration of the color and an increase of the acidity were observed in the obtained tomato purees. These preliminary results obtained on these processed products are helpful for further processing and promotion of these different varieties of locally grown tomatoes in Togo.
多哥人生产、加工和广泛消费西红柿。然而,当地的生产还不够发达,无法提供当地加工的西红柿。为了促进当地发展,刺激当地加工和减少收获后损失,在多哥用三种当地种植的番茄品种(Aklikonvi、Tohounvi和Pomvi)加工成果泥。使用了LAMICODA实验室开发的一种方法,并对该过程进行了调整,使任何当地生产者都能掌握。为了验证产品的稳定性,确定最终产品的理化和营养品质,对三个不同品种的番茄碎和番茄泥进行了微生物学和理化分析。结果显示,这些产品的卫生质量符合欧盟的标准。此外,结果表明,所生产的果泥含有番茄红素(3.94毫克至7.36毫克/100克)、维生素E(0.38毫克至1.14毫克/100克)、β-胡萝卜素(0.27毫克至0.56毫克/100克)和糖(如果糖:0.75克至1.56克/100克;葡萄糖:每100克0.78克至1.52克不等)。番茄碎和番茄泥的总糖含量差异显著(p<0.05)。在得到的番茄酱中观察到颜色的恶化和酸度的增加。这些加工产品的初步结果有助于进一步加工和推广多哥当地种植的不同品种的西红柿。
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引用次数: 0
Profiling of processors for baked and fried wheat based products in Nairobi Kenya 肯尼亚内罗毕烘焙和油炸小麦产品加工者概况
Pub Date : 2021-07-31 DOI: 10.5897/ajfs2021.2112
G. Abong’, Mukani Moyo, Elmah Odhiambo Geoffrey, T. Muzhingi
This study aimed at profiling bakers and fryers of wheat products in Nairobi to inform decisions leading towards incorporation of Orange Fleshed Sweet Potato (OFSP) puree in these products. An exhaustive sampling was carried out and 748 processors were interviewed in Nairobi County. The results indicate that even though there were more female processors than men, this difference was not significant (p>0.05). Majority of processors and consumers were youths and there was low adoption of OFSP as an ingredient (4%). However, about 78% of the processors were willing to adopt OFSP puree as an ingredient. Moreover, the youths dominated the micro-enterprises presumably because they have a lot of energy as well as huge potential for innovation. Major customers were street passerby as majority operated roadside kiosks or hotels while location had significant (p<0.05) influence on willingness to adopt OFSP, production trends, main customers as well as markets for the products. The survey recommends capacity building among the Micro-Small-Medium-Enterprises (MSMEs) in order to be able to adopt nutritious ingredients such as Orange Fleshed Sweet Potato (OFSP) puree as a way of improving food and nutrition security for the masses that rely on fried and baked products.   Key words: Bakers, fryers, orange fleshed sweet potato, micro-small-medium-enterprises, wheat products.
本研究旨在分析内罗毕小麦产品的面包师和煎炸者,为在这些产品中加入橙肉甘薯(OFSP)泥的决策提供信息。进行了详尽的抽样,并在内罗毕县采访了748名加工者。结果表明,尽管女性加工者数量多于男性,但差异不显著(p>0.05)。大多数加工商和消费者是年轻人,OFSP作为一种成分的采用率很低(4%)。然而,大约78%的加工商愿意采用OFSP果泥作为配料。此外,年轻人在微型企业中占主导地位,可能是因为他们有很多精力和巨大的创新潜力。主要客户为街道路人,主要经营路边亭或酒店,而地点对采用OFSP的意愿、生产趋势、主要客户和产品市场有显著影响(p<0.05)。该调查建议中小微企业(MSMEs)进行能力建设,以便能够采用橙肉甘薯(OFSP)泥等营养成分,以改善依赖油炸和烘焙产品的大众的食品和营养安全。关键词:面包师,油锅,红薯,中小微企业,小麦制品
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引用次数: 1
Studies on some physico-chemical and engineering properties of Musa spp (ABB) starch flour Musa spp (ABB)淀粉粉的理化及工程性能研究
Pub Date : 2021-07-31 DOI: 10.5897/ajfs2020.1921
W. A. Adebayo, B. Ogunsina, K. Taiwo, P. O. Chidoziri
This study investigated some physiochemical and engineering properties of Musa spp (ABB) starch flour using standard procedures with a view to providing data that will aid in process design, control and bulk handling of Musa spp (ABB) starch flour. Loose and packed bulk densities, the least gelation concentration, density ratio and porosity ranged between 0.47 to 0.70 g/mL, 0.60 to 0.95 g/mL, 8 to 16% (w/v), 74.25 to 77.26%, and 22.73 to 25.74% respectively. Similarly, the Carr index had a range of 29.11 to 35.78% and Hausner ratio ranged from 1.29 to 1.36. The amylose-amylopectin ratio content obtained range from 0.45 to 0.50. Also, the thermal properties of the samples measured using differential scanning calorimeter ranged between 79.3 and 92.8°C, 106.4 and 121.2°C, 106.2 and 122.8°C, 56.75 and 278.6 J/g, 21.4 and 94.743.5°C, 10.95 and 20.28 J/(gK), 0.50 and 0.705 W/m°C, and 0.090 and 0.094 m2/s for onset temperature, peak temperature, end temperature, enthalpy, temperature range, specific heat capacity, thermal conductivity and thermal diffusivity, respectively. This study therefore provides engineering data in relation to process design, control and bulk handling with a view to extending the usage of Musa spp (ABB) starch samples in food process industries.   Key words: Musa spp (ABB), Starch, physico-chemical and engineering properties, bulk handling.
本研究采用标准程序研究了Musa spp (ABB)淀粉粉的一些理化和工程特性,以期为Musa spp (ABB)淀粉粉的工艺设计、控制和批量处理提供数据。松散和堆积容重、最小胶凝浓度、密度比和孔隙度分别为0.47 ~ 0.70 g/mL、0.60 ~ 0.95 g/mL、8 ~ 16% (w/v)、74.25 ~ 77.26%和22.73 ~ 25.74%。同样,Carr指数为29.11 ~ 35.78%,Hausner比值为1.29 ~ 1.36。得到的直链淀粉-支链淀粉比含量为0.45 ~ 0.50。差示扫描量热计测得样品的热性能分别为79.3 ~ 92.8℃、106.4 ~ 121.2℃、106.2 ~ 122.8℃、56.75 ~ 278.6 J/g、21.4 ~ 94.743.5℃、10.95 ~ 20.28 J/(gK)、0.50 ~ 0.705 W/m℃、0.090 ~ 0.094 m2/s、焓、温度范围、比热容、导热系数和热扩散系数。因此,本研究提供了与工艺设计、控制和散装处理相关的工程数据,以期扩大Musa spp (ABB)淀粉样品在食品加工工业中的使用。关键词:Musa spp (ABB),淀粉,理化和工程性质,散装处理
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引用次数: 0
Effects of freezing as a post-harvest storage technique on quality of Friesian crossbred cattle milk 冷冻作为采后贮藏技术对杂交牛奶品质的影响
Pub Date : 2021-07-31 DOI: 10.5897/ajfs2021.2118
Lilian Mutheu Musembei, R. Bett, C. Gachuiri, Benjamin Kyalo Mbondo, F. M. Kibegwa
Twenty-five raw milk samples from Friesian crossbred dairy cows were analyzed for milk fat, protein and lactose using an ultrasonic milk analyzer. The samples were then subjected to two different freezing protocols; single freezing and multiple freezing, after which parameters were reanalyzed after freezing and thawing at different freezing times (24, 48, and 72 h). Paired t-test was used to compare the effect of freezing type while the differences in milk constituents with freezing time were analyzed using ANOVA. Linear regression analysis was also performed to study correlations between freezing duration and any change in cattle milk's macronutrient content. The results indicated that milk fat, protein and lactose content decreased significantly with freezing time. However, the decrease was more in multiple frozen samples than single frozen samples. The most decreased macronutrients were lactose (14.1%) in single freezing and fat (25.5%) in multiple freezing. Analysis of the interaction between freezing type and freezing time showed that freezing time significantly affected all the parameters while freezing type (p=0.03) and its interaction with freezing time (p=0.02) affected only the fat content. In conclusion, it should be noted that cattle milk samples frozen at -20°C leads to a significant decrease in fat, protein, and lactose content. The loss of constituents was much more pronounced when samples were frozen, thawed, and refrozen (multiple freezing) than when samples were thawed only once (single freezing).   Key words: Single freezing, multiple freezing, macronutrients, dairy cattle.
采用超声牛奶分析仪对25头弗里西亚杂交奶牛的原料奶样品进行了乳脂、蛋白质和乳糖的分析。然后将样品进行两种不同的冷冻方案;单次冷冻和多次冷冻后,分别在不同的冷冻时间(24、48和72 h)冷冻和解冻后重新分析参数。采用配对t检验比较冷冻类型的影响,采用方差分析分析牛奶成分随冷冻时间的差异。通过线性回归分析,研究了冷冻时间与牛乳中常量营养素含量变化之间的相关性。结果表明,随着冷冻时间的延长,乳脂、蛋白质和乳糖含量显著降低。然而,多个冷冻样品的下降幅度大于单个冷冻样品。其中,单次冷冻减少最多的是乳糖(14.1%),多次冷冻减少最多的是脂肪(25.5%)。冻结方式与冻结时间的交互作用分析表明,冻结时间对各参数均有显著影响,而冻结方式(p=0.03)及其与冻结时间的交互作用(p=0.02)仅对脂肪含量有影响。综上所述,应该注意的是,在-20°C冷冻的牛乳样品中,脂肪、蛋白质和乳糖含量显著降低。当样品被冷冻、解冻和再冷冻(多次冷冻)时,成分的损失要比样品只解冻一次(单次冷冻)时明显得多。关键词:单次冷冻,多次冷冻,常量营养素,奶牛
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引用次数: 1
期刊
African Journal of Food Science
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