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Miniaturisation of the Daphnia magna immobilisation assay for the reliable testing of low volume samples. 小型水蚤大固定化试验的可靠测试小体积样品。
Pub Date : 2025-01-29 eCollection Date: 2025-01-01 DOI: 10.14324/111.444/ucloe.3037
Eberhard Küster, George Gyan Addo, Silke Aulhorn, Dana Kühnel

International standard test guidelines for the ecotoxicological characterisation of various substances use organisms such as algae, daphnids and fish embryos. These guidelines recommend or use relatively high volumes of water for the process of testing, for example, 200 mL for a complete dose-response relationship in a daphnia assay. However, for various samples such as concentrated extracts from environmental monitoring or leachates from microplastic ageing experiments, the amount of available sample volume is limited, that is, rather in the range of 10-50 mL/biotest. Using the exposure volumes as recommended in test guidelines would not allow to test a range of different concentrations or to repeat tests or use multiple different organismic bioassays. Lower media volumes would allow the testing of more samples (more concentrations per sample, more test repetitions for statistical robustness, etc.) but it may also decrease the possible number of organisms tested in the same volume. Here, we aimed at reducing the test volumes in the acute daphnia assay (using a maximum of 30 mL for a complete dose-response relationship) without impacting animals' sensitivity towards toxicants. A literature review on existing miniaturisation approaches was used as a starting point. Subsequently, assays employing conventional as well as reduced test volumes were compared for 16 selected test substances with a diverse spectrum of lipophilicity. Results showed that there are differences in EC50 between the two approaches, but that these differences were overall only within a range of a factor of two to three. Further, by retrieving EC50 values for the genus Daphnia and 16 test substances from the United States Environmental Protection Agency database, we demonstrated that our results are well in line with the general differences in sensitivities.

各种物质的生态毒理学特征的国际标准测试指南使用生物体,如藻类,水蚤和鱼胚胎。这些指南建议或在测试过程中使用相对大体积的水,例如,在水蚤测定中使用200毫升的水来建立完整的剂量-反应关系。然而,对于各种样品,如环境监测的浓缩提取物或微塑料老化实验的渗滤液,可用的样本量是有限的,即在10-50 mL/biotest的范围内。使用测试指南中建议的暴露量将不允许测试一系列不同的浓度或重复测试或使用多种不同的有机生物测定。较低的培养基体积将允许测试更多的样品(每个样品的浓度更高,为了统计稳健性而进行更多的测试重复,等等),但它也可能减少在相同体积中测试的生物体的可能数量。在这里,我们的目标是在不影响动物对毒物的敏感性的情况下减少急性水蚤试验的测试体积(使用最大30ml来完成完整的剂量-反应关系)。文献综述现有的小型化方法被用作起点。随后,对16种具有不同亲脂性谱的选定测试物质进行了采用常规和减少测试体积的分析比较。结果表明,两种方法之间的EC50存在差异,但这些差异总体上仅在2到3个因素的范围内。此外,通过从美国环境保护署数据库中检索水蚤属和16种测试物质的EC50值,我们证明了我们的结果与敏感性的一般差异是一致的。
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引用次数: 0
A virtual global carbon price is essential to drive rapid decarbonisation. 虚拟的全球碳价格对于推动快速脱碳至关重要。
Pub Date : 2024-12-17 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.1983
Richard H Clarke, Mark A Maslin

Dealing with climate change is now an infrastructure challenge. Within the next 30 years our energy generation must switch from fossil fuels to renewables. New buildings need to be zero-carbon and existing buildings need to be retrofitted. Our global transportation network will need to be transformed. Delivering the Net Zero World is an engineering challenge. But to do this we need a globally agreed virtual carbon price so that every single infrastructure project can be assessed in terms of its impact on carbon emissions and thus planetary health. We propose a loss-and-damage-based carbon price that is enhanced or reduced by variable, national impact factors. Carbon intensity weighting would further increase the price's impact.

应对气候变化现在是一项基础设施挑战。在未来30年内,我们的能源生产必须从化石燃料转向可再生能源。新建筑需要零碳排放,现有建筑需要翻新。我们的全球交通网络需要转型。实现净零世界是一项工程挑战。但要做到这一点,我们需要一个全球一致同意的虚拟碳价格,这样每个基础设施项目都可以根据其对碳排放的影响来评估,从而评估地球健康。我们提出了一种基于损失和损害的碳价格,该价格受可变的国家影响因素的影响而提高或降低。碳强度加权将进一步增加价格的影响。
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引用次数: 0
Urinary arsenic species and birth outcomes in Tacna, Peru, 2019: a prospective cohort study. 2019年秘鲁塔克纳市尿砷种类和出生结局:一项前瞻性队列研究。
Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.3146
Diego Fano-Sizgorich, Matthew O Gribble, Cinthya Vásquez-Velásquez, Claudio Ramírez-Atencio, Julio Aguilar, Jeffrey K Wickliffe, Maureen Y Lichtveld, Dana B Barr, Gustavo F Gonzales

Arsenic exposure during pregnancy might affect foetal development. Arsenic metabolism may modulate the potential damage to the fetus. Tacna has the highest arsenic exposure levels in Peru. However, this region also has the highest birth weight in Peru. It is not known if arsenic exposure is affecting maternal-perinatal health in Tacna. This study aimed to evaluate the association between urinary arsenic metabolism and birth outcomes, specifically birth weight and gestational age at birth in Tacna, Peru. A prospective cohort study was conducted, involving 158 pregnant women in Tacna, Peru, during January-November 2019. Participants were enrolled in their second trimester and followed-up until birth. Urine samples were collected in the second and third trimesters. Urine samples were analysed for total arsenic concentration and its species. Generalised estimating equations analysis was used to evaluate the association of interest. Inter-differences in arsenic toxicokinetics, calculated with principal component analysis was included as an interaction term. Analysis was stratified by pregnancy trimester. The median total urinary arsenic concentration was 33.34 μg/L. Inorganic arsenic and dimethylarsinic acid were higher in the second trimester. Dimethylarsinic acid was the predominant component (84.78% of total urinary arsenic). No significant association was found between urinary arsenic exposure and birth weight or gestational age at birth. The association was not affected by arsenic metabolism. Stratified analyses by pregnancy trimester also showed no significant associations. Urinary arsenic was not associated with birth weight, and this null relationship remained unaffected by arsenic toxicokinetic differences reflected in urine.

怀孕期间接触砷可能影响胎儿发育。砷代谢可调节对胎儿的潜在损害。塔克纳是秘鲁砷含量最高的地区。然而,这个地区也是秘鲁出生体重最高的地区。目前尚不清楚砷暴露是否正在影响塔克纳的产妇和围产期健康。本研究旨在评估尿砷代谢与出生结局之间的关系,特别是在秘鲁塔克纳出生时的出生体重和胎龄。2019年1月至11月期间,在秘鲁塔克纳进行了一项前瞻性队列研究,涉及158名孕妇。参与者在妊娠中期登记,并随访至出生。在妊娠中期和晚期采集尿样。尿样分析总砷浓度及其种类。使用广义估计方程分析来评估兴趣关联。用主成分分析计算的砷中毒动力学的相互差异被包括作为相互作用项。分析按妊娠期进行分层。尿中总砷浓度中位数为33.34 μg/L。无机砷和二甲基硅酸在妊娠中期较高。二甲基胂酸为主要成分,占尿砷总量的84.78%。尿砷暴露与出生体重或出生时胎龄之间没有显著关联。这种关联不受砷代谢的影响。妊娠三个月的分层分析也显示无显著关联。尿砷与出生体重无关,这种零关系不受尿中砷毒性动力学差异的影响。
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引用次数: 0
Hydrophobic treatments and their application with internal wall insulation. 憎水处理及其在内墙隔热中的应用。
Pub Date : 2024-11-08 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.1978
Toby Cambray, Valentina Marincioni, Hector Altamirano

Hydrophobic (or water repellent) treatments have been proposed to mitigate moisture risks associated with internal wall insulation when applied to solid masonry walls. This can reduce risks associated with moisture accumulation within the structure such as mould growth or the deterioration of joist ends and other embedded timber. Where treatments perform well there is a net reduction of moisture content and risk. However, such treatments slow down drying processes, and therefore may result in a net increase in moisture if the treatment is bypassed by, for example, cracks. Some treatments may lead to damage to external masonry surfaces in some situations. Freeze-thaw and salt crystallisation are the two main causes. Hygrothermal simulations may give some indication of risks but techniques to assess the risk of surface damage are either simplistic, impractical outside of the research environment or both. This paper reviews the current field of assessing and predicting the risk of surface damage associated with surface treatments.

憎水(或憎水)处理已被提出来用于减轻与实心砌体墙的内墙保温有关的潮湿风险。这可以降低与结构内湿气积聚有关的风险,如霉菌生长或托梁末端和其他嵌入木材的老化。在处理效果良好的情况下,湿气含量和风险会净减少。但是,这些处理方法会减缓干燥过程,因此,如果处理方法被裂缝等绕过,可能会导致湿度净增加。在某些情况下,某些处理方法可能会导致外部砖石表面受损。冻融和盐结晶是两个主要原因。湿热模拟可以在一定程度上提示风险,但评估表面损坏风险的技术要么过于简单,要么在研究环境之外不切实际,要么两者兼而有之。本文回顾了目前与表面处理相关的表面损伤风险评估和预测领域。
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引用次数: 0
The causes of air movement in hidden indoor micro-environments: measurements in historic bookshelves. 隐蔽室内微环境中空气流动的原因:对历史书架的测量。
Pub Date : 2024-09-25 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.1976
Morena Ferreira, Josep Grau-Bové, Nigel Blades, Lisa O'Hagan, Hector Altamirano

The use of ventilation holes in small micro-environments has been proposed by the National Trust as a mechanism to improve the environmental conditions of moisture and temperature within bookshelves. At one National Trust historic property, this mechanism has been used to encourage air movement behind books as a possible strategy to reduce the risk of mould growth. It is believed that including ventilation holes as a passive design solution to promote airflow within micro-environments could prevent decay from occurring in the archives of historic buildings. This paper investigates the mechanisms that cause airflow behind bookshelves using field measurements in three National Trust historic libraries. The measurements indicate that small but measurable velocities, up to 4 cm/s, can be passively generated behind bookshelves. Air movement in such confined micro-environments is probably caused by a combination of natural convection, caused by temperature differences between the walls and the interior and the exterior of the bookshelf, and forced convection due to drafts in the surrounding environment. While in some cases one mechanism prevailed, both mechanisms may be present simultaneously in most cases. Further research is needed to clarify how surface temperature drives air motion behind shelves.

国民托管组织提出在小型微环境中使用通风孔,作为改善书架内湿度和温度环境条件的一种机制。在国家信托基金的一处历史遗产中,这种机制被用来促进书本后面的空气流动,作为降低霉菌生长风险的一种可能策略。人们相信,将通风孔作为一种被动的设计方案来促进微环境中的空气流动,可以防止历史建筑档案发生腐烂。本文通过对三座国民托管历史图书馆的实地测量,研究了造成书架背后气流的机理。测量结果表明,书架后可以被动地产生微小但可测量的速度,最高可达 4 厘米/秒。这种密闭微环境中的空气流动可能是由墙壁和书架内外温差引起的自然对流和周围环境气流引起的强制对流共同造成的。虽然在某些情况下一种机制占主导地位,但在大多数情况下两种机制可能同时存在。要弄清表面温度是如何驱动书架后面的空气运动的,还需要进一步的研究。
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引用次数: 0
Moisture buffering and mould growth characteristics of naturally ventilated lime plastered houses. 自然通风石灰抹灰房屋的水分缓冲和霉菌生长特性。
Pub Date : 2024-09-10 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.1988
Vismaya Paralkar, Rashmin Damle

Lime plaster is a sustainable building material that can be an effective passive cooling strategy. The moisture buffering quality of lime causes adsorption and desorption of moisture which moderates the indoor relative humidity. Its vapour permeability is also influential in moisture transfer across the building envelope. Lime plaster also has a self-healing quality which prevents the formation of inner cracks. Moreover, its strength increases with time leading to a longer life span. In old structures, an important function is the breathability of ceilings and walls. Hence, it is often used in conservation projects where it improves the appearance and durability of old buildings. Often organic additives employed to impart certain qualities to the lime mortar/plaster led to mould growth. Mould growth degrades indoor air quality, and the occupant health is compromised. To avoid mould-related problems, it is necessary to understand the behaviour of lime plaster with respect to the indoor relative humidity and surface moisture content. This paper focuses on the hygrothermal performance of lime plaster in naturally ventilated residential spaces. Surveys were carried out in 45 traditional buildings in Ahmedabad in India with measurements of ambient variables, such as temperature, relative humidity, wall moisture content, etc. The mould growth patterns of these spaces are related to the measured variables and wall characteristics. Hygrothermal simulations of some spaces were also carried out to observe the moisture buffering of lime plaster. Experimental observations were then compared to simulation results to see if the predictions of the hygrothermal models were realistic.

石灰抹灰是一种可持续的建筑材料,可以作为一种有效的被动降温策略。石灰的湿气缓冲特性可以吸附和解吸湿气,从而调节室内相对湿度。它的透汽性也会影响湿气在建筑围护结构中的传递。石灰抹灰还具有自愈性,可防止内部裂缝的形成。此外,它的强度会随着时间的推移而增加,从而延长使用寿命。在古建筑中,天花板和墙壁的透气性是一项重要功能。因此,它经常被用于保护项目中,以改善古建筑的外观和耐久性。为了赋予石灰砂浆/灰泥某些品质而使用的有机添加剂往往会导致霉菌滋生。霉菌生长会降低室内空气质量,影响居住者的健康。为了避免与霉菌有关的问题,有必要了解石灰抹灰在室内相对湿度和表面含水量方面的表现。本文重点研究了自然通风住宅空间中石灰抹灰的湿热性能。在印度艾哈迈达巴德的 45 栋传统建筑中进行了调查,测量了温度、相对湿度、墙壁含水率等环境变量。这些空间的霉菌生长模式与测量变量和墙体特征有关。还对一些空间进行了湿热模拟,以观察石灰抹灰的湿度缓冲作用。然后将实验观察结果与模拟结果进行比较,以确定湿热模型的预测是否符合实际情况。
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引用次数: 0
Responding to the environmental emergency through education: the imperative for teacher support across all subjects. 通过教育应对环境紧急状况:必须为所有学科的教师提供支持。
Pub Date : 2024-07-24 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.1987
Kate Greer, Nicola Walshe, Alison Kitson, Justin Dillon

The most recent Intergovernmental Panel on Climate Change report sets out sobering scenarios about the future for our young people and appeals for 'deep, rapid, and sustained reductions in greenhouse gas emissions'. Although technological responses are essential to achieve these reductions, technocratic solutions alone will not solve the environmental emergency; a widespread societal transformation is needed. Education can play a profound role in this transformation as it is fundamental to building a society with knowledge, skills and motivation to tackle climate change so as to regenerate ecological and social systems. This commentary reflects on multiple dimensions of education's role, focusing particularly on schools and the important contribution that all subjects can make towards developing interdisciplinary, complex understandings of the environmental emergency and living more sustainably. Drawing from a recent nationwide survey of teachers in England carried out by the UCL Centre for Climate Change and Sustainability Education, we highlight a troubling lack of engagement in formal professional development related to climate change and sustainability, even amongst a 'climate change engaged' cohort of teachers, and the imperative for comprehensive professional learning for teachers from across all subjects and year levels.

政府间气候变化专门委员会的最新报告对我们年轻人的未来提出了令人警醒的设想,并呼吁 "深入、迅速和持续地减少温室气体排放"。尽管技术应对措施对实现这些减排至关重要,但仅靠技术官僚的解决方案并不能解决环境紧急状况;还需要广泛的社会变革。教育可以在这一转变中发挥深远的作用,因为它是建设一个具备应对气候变化的知识、技能和动力的社会,从而实现生态和社会系统再生的根本。本评论从多个方面反思了教育的作用,尤其关注学校以及所有学科在培养跨学科、对环境紧急情况的复杂理解和更可持续地生活方面所能做出的重要贡献。根据伦敦大学洛杉矶分校气候变化与可持续发展教育中心(UCL Centre for Climate Change and Sustainability Education)最近对英格兰教师进行的一项全国性调查,我们强调,即使是在 "参与气候变化 "的教师群体中,也缺乏与气候变化和可持续发展相关的正规专业发展,这一点令人担忧。
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引用次数: 0
Enabling interdisciplinary research capacity for sustainable development: self-evaluation of the Blue Communities project in the UK and Southeast Asia. 提高跨学科研究能力,促进可持续发展:英国和东南亚蓝色社区项目的自我评估。
Pub Date : 2024-07-04 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.1970
Fiona Culhane, Victoria Cheung, Melanie Austen

Global challenges such as climate change, food security and human health and well-being disproportionately impact people from low-income countries. These challenges are complex and require an international and transdisciplinary approach to research, with research skills and expertise from different disciplines, sectors and regions. In addressing this, a key goal of the research project, Blue Communities, was to create and expand mutual interdisciplinary capacity of both United Kingdom and Southeast Asian Partners. An existing questionnaire on research capacity was uniquely adapted to include interdisciplinary and international aspects and distributed for the first time as an online survey to the participants of the Blue Communities project comprising researchers across all career stages. Participants were asked about their perceptions of the research capacity and culture of their organisation, team and self and whether they believed any aspects have changed since their involvement with the project. Greatest improvement was seen at the self-level where results indicated a positive relationship between an individual's current success or skill and their improvement over the course of the research project across 18 out of 22 aspects of research capacity for Southeast Asian, and two for UK respondents. The conflict between achieving research aims, building research capacity and making societal impact was evident. Institutional support is required to value these core aspects of interdisciplinary research.

气候变化、粮食安全、人类健康和福祉等全球性挑战对低收入国家的人民造成了极大的影响。这些挑战错综复杂,需要采用国际和跨学科的研究方法,利用来自不同学科、部门和地区的研究技能和专业知识。为了解决这个问题,研究项目 "蓝色社区 "的一个关键目标是建立和扩大联合王国和东南亚合作伙伴的跨学科能力。对现有的研究能力调查问卷进行了独特的调整,以纳入跨学科和国际方面的内容,并首次以在线调查的形式分发给蓝色社区项目的参与者,其中包括各个职业阶段的研究人员。调查询问了参与者对其所在组织、团队和自我的研究能力和文化的看法,以及他们是否认为自参与该项目以来任何方面都发生了变化。结果显示,在东南亚受访者研究能力的 22 个方面中,有 18 个方面的个人目前的成功或技能与他们在研究项目过程中的进步之间存在正相关关系,而在英国受访者的 22 个方面中,有两个方面的个人目前的成功或技能与他们在研究项目过程中的进步之间存在正相关关系。实现研究目标、建设研究能力和产生社会影响之间的冲突显而易见。要重视跨学科研究的这些核心方面,就需要机构的支持。
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引用次数: 0
Correction to: Risk perception and use of personal care products by race and ethnicity among a diverse population. 更正:按种族和民族分列的不同人群对个人护理产品的风险认知和使用情况。
Pub Date : 2024-06-26 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.3038.CORR
Julia Mandeville, Zeina Alkhalaf, Charlotte Joannidis, Michelle Ryan, Devon Nelson, Lesliam Quiros-Alcala, Matthew O Gribble, Anna Z Pollack

[This corrects the article DOI: 10.14324/111.444/ucloe.3038.].

[此处更正了文章 DOI:10.14324/111.444/ucloe.3038.]。
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引用次数: 0
Promoting planting in front gardens: a systematic approach to intervention development. 促进前庭种植:制定干预措施的系统方法。
Pub Date : 2024-05-24 eCollection Date: 2024-01-01 DOI: 10.14324/111.444/ucloe.3147
Ayşe Lisa Allison, Rachael Frost, Niamh Murtagh

Planting in front gardens is associated with a range of human and environmental health benefits. Effective interventions aimed at cultivating this practice are, however, hampered by the paucity of theory- and evidence-based behavioural research in this context. This study aims to systematically determine a set of behaviour change interventions likely to be effective at promoting planting in front gardens amongst UK householders. The Behaviour Change Wheel framework was applied. Behavioural systems mapping was used to identify community actors relevant to front gardening. Potential behavioural influences on householders' front gardening were identified using the Capability, Opportunity, Motivation, Behaviour model. Using peer-reviewed scientific findings as evidence, behavioural influences were systematically linked to potential intervention strategies, behaviour change techniques and real-world implementation options. Finally, intervention recommendations were refined through expert evaluations and local councillor and public stakeholder feedback, evaluating them against the Acceptability, Practicability, Effectiveness, Affordability, Side effects and Equity criteria in a UK implementation context. This study formulated 12 intervention recommendations, implementable at a community level, to promote front gardening. Stakeholder feedback revealed a preference for educational and supportive (social and practical) strategies (e.g., community gardening workshops, front gardening 'starter kits') over persuasive and motivational approaches (e.g., social marketing, motivational letters from the council to householders). Householders' front gardening behaviour is complex and influenced by the behaviour of many other community actors. It also needs to be understood as a step in a continuum of other behaviours (e.g., clearing land, gardening, waste disposal). This study demonstrates the application of behavioural science to an understudied implementation context, that is, front gardening promotion, drawing on a rigorous development process promoting a transparent approach to intervention design. Stakeholder consultation allowed relevance, feasibility and practical issues to be considered. These improve the likely effectiveness of interventions in practice. The next steps include evaluating the proposed interventions in practice.

在庭院前种植植物与一系列人类和环境健康益处有关。然而,由于缺乏相关的理论和循证行为研究,旨在培养这种习惯的有效干预措施受到了阻碍。本研究旨在系统地确定一套可能有效的行为改变干预措施,以促进英国户主在前院种植植物。研究采用了行为改变轮框架。采用行为系统图来确定与前庭种植相关的社区参与者。利用能力、机会、动机、行为模型,确定了影响住户前庭种植的潜在行为因素。以同行评审的科学发现为证据,系统地将行为影响因素与潜在的干预策略、行为改变技术和实际实施方案联系起来。最后,通过专家评估以及地方议员和公众利益相关者的反馈,对干预建议进行了完善,并根据英国实施背景下的可接受性、实用性、有效性、可负担性、副作用和公平性标准对其进行了评估。这项研究提出了 12 项可在社区层面实施的干预建议,以推广门前园艺。利益相关者的反馈显示,他们更倾向于采用教育和支持性(社会和实践)策略(如社区园艺研讨会、前庭园艺 "入门套件"),而不是说服和激励性方法(如社会营销、议会致住户的激励性信件)。住户的门前园艺行为是复杂的,受到许多其他社区参与者行为的影响。还需要将其理解为其他行为(如清理土地、园艺、废物处理)连续统一体中的一个步骤。本研究展示了行为科学在前沿园艺推广这一研究不足的实施环境中的应用,并借鉴了严格的开发流程,促进了透明的干预设计方法。通过征求利益相关者的意见,对相关性、可行性和实际问题进行了考虑。这些都提高了干预措施在实践中的有效性。接下来的步骤包括在实践中评估拟议的干预措施。
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引用次数: 0
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