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Changes in tropomyosin during primary culture of embryonic myocardiocytes 胚胎心肌细胞原代培养过程中原肌球蛋白的变化
Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80064-0
M.A. Muros, A.E. Aránega, C. Vélez, F.J. González, J.E. Fernández, L. Alvarez, A. Aránega

We chose the Hamburger and Hamilton's stage 29 (HH 29) to investigate the expression of tropomyosin in chick myocardiocytes during 14 days on culture. Throughout 14 days of cell culture, changes in cell morphology were accompained by a redistribution of tropomyosin in different cell compartments. We used FACScan, SDS-PAGE and densitometric analysis to quantify total cell tropomyosin and concentrations of this protein in different cell fractions. Tropomyosin was found mostly in the cytoskeletal fraction than in the cytoplasmic.

When we compared the densitometric values from SDS-PAGE of cells in different stages of development we found that in HH 19, tropomyosin was more abundant in the cytoplasmic than in the cytoskeletal fraction. By HH 29, the two fractions had become inverted, and in HH 39, tropomyosin was clearly more abundant in the cytoskeletal than in the cytoplasmic fraction.

In the IFI analysis, tropomyosin was found to label the Stress fiberlike structures (SFL) in different patterns depending on the area of the cell which expressed this protein.

本实验选用汉堡和汉密尔顿29期(HH 29)鸡心肌细胞,观察培养14 d后原肌球蛋白在鸡心肌细胞中的表达情况。在14天的细胞培养过程中,细胞形态的变化伴随着原肌球蛋白在不同细胞区室的重新分布。我们使用FACScan, SDS-PAGE和密度分析来定量细胞原肌球蛋白总量和该蛋白在不同细胞组分中的浓度。原肌球蛋白主要存在于细胞骨架部分,而不在细胞质中。当我们比较不同发育阶段细胞的SDS-PAGE密度值时,我们发现在HH 19中,原肌球蛋白在细胞质中比在细胞骨架部分中更丰富。到HH 29时,这两个部分已经倒置,在HH 39时,原肌球蛋白在细胞骨架中的含量明显高于细胞质部分。在IFI分析中,发现原肌球蛋白根据表达该蛋白的细胞区域以不同的模式标记应力纤维样结构(SFL)。
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引用次数: 2
Thapsigargin, an inhibitor of Ca2+-ATPase, antagonizes vacuole formation of hepatoma cells induced by teleocidin Thapsigargin是一种Ca2+- atp酶抑制剂,可拮抗远杀素诱导的肝癌细胞液泡形成
Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80069-X
Ayumi Tsukamoto, Yoshiyasu Kaneko, Kiyoshi Kurokawa
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引用次数: 1
The interaction of staphylococcal protein a with free coelomocytes of annelids 葡萄球菌蛋白a与环节动物游离腔胚细胞的相互作用
Pub Date : 1992-05-01 DOI: 10.1016/S0309-1651(06)80067-6
Martin Bilej, Jaroslav Rejnek, Ludmila Tučková

The Staphylococcal protein A (SpA) binding protein was detected on the surface of annelid coelomocytes. The flow cytometric analysis revealed that 50 % coelomocytes of Lumbricus terrestris react with SpA, a figure six times higher than the number of positive coelomocytes found in Eisenia foetida.

在环节动物体腔细胞表面检测到葡萄球菌蛋白A (SpA)结合蛋白。流式细胞术分析显示,50%的地蚓体腔细胞与SpA发生反应,这一数字是爱森ia foetida体腔细胞阳性数量的6倍。
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引用次数: 3
LM Franks和NM Teich,编辑,介绍癌症的细胞和分子生物学(第二版),牛津大学出版社,剑桥(1991)ISBN 0 19 854734 X。
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80147-5
P Monaghan
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引用次数: 0
Constituent cell biology societies 组成细胞生物学学会
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80149-9
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引用次数: 0
Effects of spermidine synthase inhibition in cultured chick embryo fibroblasts 抑制鸡胚成纤维细胞亚精胺合酶的作用
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80140-2
A. Caruso , A. Pellati , P. Bosi , N. Arena , G. Stabellini

The administration of bis-cyclohexylammonium sulfate (BCHS), a spermidine synthase inhibitor, to in vitro cultures of chick embryo fibroblasts caused a decrease in cellular spermidine levels and an increase in putrescine and spermine. Cell proliferation rate and DNA synthesis were also inhibited. As protein synthesis did not change, it would seem that low levels of cellular spermidine inhibit cell growth depressing DNA synthesis.

将亚精胺合成酶抑制剂双环己基硫酸铵(BCHS)用于鸡胚成纤维细胞体外培养,可导致细胞亚精胺水平降低,腐胺和精胺水平升高。细胞增殖率和DNA合成也受到抑制。由于蛋白质合成没有改变,低水平的细胞亚精胺似乎抑制了细胞生长,抑制了DNA合成。
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引用次数: 5
Meetings and conferences 会议和会议
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80148-7
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引用次数: 0
Proliferation-dependency of γ-glutamyl hydrolase activity in various mouse cells γ-谷氨酰水解酶活性在多种小鼠细胞中的增殖依赖性
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80142-6
Ewa Sikora, Bozena Kaminska, Barbara Grzelakowska-Sztabert

The activity of γ-glutamyl hydrolase, the enzyme which deglutamylates folyl and antifolyl polyglutamates, changed significantlly in mouse cells during different phases of growth, being about two times lower in actively proliferating mice splenocytes and fibroblasts than in nondividing cells. In EAC cells growing in vivo the lowest activity was observed in cells in the logarythmic phase. Methotrexate treatment of mice in a dose of 500 mg/kg body weight increased the activity of the enzyme in EAC cells about 1.5 times. We suggest that γ-glutamyl hydrolase is a proliferating dependent enzyme which together with folylpolyglutamate synthetase ensures in cells an appriopriate amount of folates in the form of polyglutamates necessary for optimizing folate-dependent biosynthetic activities.

γ-谷氨酰水解酶(γ-谷氨酰水解叶酸和抗叶酸多谷氨酸的酶)的活性在小鼠细胞生长的不同阶段发生显著变化,活跃增殖的小鼠脾细胞和成纤维细胞的活性比非分裂细胞的活性低约2倍。在体内生长的EAC细胞中,对数节律期的细胞活性最低。剂量为500 mg/kg体重的甲氨蝶呤处理小鼠EAC细胞酶活性增加约1.5倍。我们认为γ-谷氨酰水解酶是一种增殖依赖酶,它与叶酸聚谷氨酸合成酶一起确保细胞中以聚谷氨酸形式的适当数量的叶酸,这是优化叶酸依赖的生物合成活性所必需的。
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引用次数: 1
Expression of SV40 T-antigen in lipoma cells with a chromosomal translocation T(3;12) is not sufficient for direct immortalization 在染色体易位T的脂肪瘤细胞中表达SV40 T抗原(3;12)并不足以实现直接永生化
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80139-6
Gazanfer Belge, Bernd Kazmierczak, Kerstin Meyer-Bolte, Sabine Bartnitzke, Jörn Bullerdiek

12q13-15 changes are the most frequent cytogenetic abnormalities in human tumor cells. To test their biological significance we used an assay based on lipoma cells with a limited in vitro lifetime and this type of chromosomal aberration. Lipoma cells with a reciprocal translocation t(3;12)(q28;q14) were transformed by transfection with a plasmid containing the SV40 “early region”. The transformed cells showed an altered morphology with loss of contact inhibition, formation of foci, and T-antigen expression. They were immortalized after a growth crisis. The karyotypic patterns before and after the crisis show that the translocation together with expression of SV40 T-antigen is not sufficient for direct immortalization.

12q13-15的改变是人类肿瘤细胞中最常见的细胞遗传学异常。为了测试它们的生物学意义,我们使用了一种基于体外寿命有限的脂肪瘤细胞和这种类型的染色体畸变的测定方法。通过转染含有SV40“早期区”的质粒,转化具有互易易位t(3;12)(q28;q14)的脂肪瘤细胞。转化后的细胞形态发生改变,失去接触抑制,形成病灶,t抗原表达。在一场增长危机之后,它们不朽了。危机前后的核型模式表明,易位和SV40 t抗原的表达不足以实现直接永生化。
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引用次数: 19
Pub Date : 1992-04-01 DOI: 10.1016/S0309-1651(06)80145-1
GRTB
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引用次数: 0
期刊
Cell biology international reports
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