Chirurgisches Forum fur experimentelle und klinische Forschung最新文献

An acute pancreatitis was induced in 20 Göttinger mini pigs by implantation of a T-tube and application of contrast medium. This process was demonstrated by laboratory and microscopic findings. Continuous infusion (n=11) of 2500 mcg somatostatin (GHIF) over 10 h showed a significant reduction of amylase and blood glucose level corresponding to the microscopic findings. The group of animals treated with somatostatin (GHIF) showed a ratio of 1:5 between necrotic and unaffected parenchymal tissues. By contrast, the histologic findings of the untreated group showed a ratio of 1:1. The investigations of blood glucose, gastrin and glucagon levels showed a rebound effect after ending the treatment with somatostatin.

Guinea pigs were orally and subcutaneously immunized against Pseudomonas aeruginosa. Whereas 93% of untreated control animals died after a subsequent IP infection, vaccinated animals developed an efficient protection. The mortality rate in orally pretreated animals was only 20% - 30%. Some immunological parameters were also improved by vaccination. IgG and IgM concentrations in serum increased, as did the agglutination titer against bacteria. Oral vaccination with heat inactivated pathogenic bacteria seems to be a promising procedure to improve the resistance of patients to bacterial infections.

Bone fractures treated with an external fixator tend to heal more slowly than with an internal fixator, since the tension of the fixator gradually relaxes and this decrease in pressure is not counteracted in any way. A dynamometer makes possible an exact measurement of effective compressive forces and an adjustment of the tension of the fixator. A special feature of the apparatus is an elastic metal ring. The apparatus is mounted on the free ends of both tubes of the fixator for measuring and adjusting the tension. This should be done once a week. Supported fractures are put under an axial compression of 50 kp. In the case of comminuted and defect fractures, and axial compression of 5 kp should be begun after 5 weeks, and this dosage should be increased every week.

In five anesthetized cats the excretion of Tc-99m-DAIDA into the gallbladder and the small bowel and the d.g. reflux were visualized by a gamma camera and analyzed by a digital computer. A d.g. reflux index was calculated following stimulation of the gallbladder by ceruletide and test meals, before and after a Heineke-Mikulicz pyloroplasty. It could be demonstrated that tracing of bile with TC-99m-DAIDA can be used as a noninvasive method for the evaluation of the d.g. reflux. Gastric intubation increases the d.g. reflux rate. Ceruletide is a much more effective choleretic agent than test meals. Despite a wide pyloroplasty neither an augmented d.g. reflux nor gastritis was seen, probably due to the preserved antral innervation.

The disadvantages of portacaval end-to-side shunt concerning hepatic function can be avoided by additional portal arterialization in healthy liver and cirrhotic rats. The maintenance of total liver perfusion is the cause of good RES clearance, nearly normal capacity of synthetic functions and normal biliary excretion. Flow and thereby pressure-adapted technique of arterialization is necessary.

Report on three different operative methods on the colon and rectum as basis for studies of the pathogenesis of colorectal cancer. Resection and colostomy predispose to cancer growth at the anastomosis or colostomy after application of 1,2 dimethylhydrazine.

Two different anchorage systems of carbon fiber strands were tested on 16 human cadaver knees. To pull the C-strands out of the scale and channel anchorage almost the same force was necessary as to break the natural collateral ligaments. The ingrowth of connective tissues and newly formed bone around the C-fibers was studied in 11 sheep. A biomechanical strength test revealed, 3 months after implantation, a 60% strength compared to the natural ligaments. Histologically a C-fiber connective tissue composite with collagen fibers was found. Newly formed bone closely surrounded the carbon. These results indicate that carbon fiber strands are suitable for ligament replacement, which allows early joint motion. Good long-term results could be assumed as a result of the high biocompatibility.