Pub Date : 2024-05-07DOI: 10.20473/j.djmkg.v57.i2.p74-79
Ashlianne Nelson, A. B. Santosh, T. Jones
Background: Oxidative stress results from the imbalance between reactive oxygen species (ROS) and cellular antioxidant defenses. Oxidative stress can cause damage to cellular structures such as cell membranes, lipids, proteins, lipoproteins, and deoxyribonucleic acids when free radicals are present in excess. Antioxidant mechanisms protect tissues against oxidative stress by decreasing free radical levels in cells through the inhibition of activities or expression of free radical-generating enzymes. Purpose: The purpose of this paper is to discuss the role of antioxidants in protecting cells from the harmful effects of ROS, particularly in the context of oral mucosa, and to clarify which molecules disrupt the pathway of these antioxidants. Review: Salivary peroxidase, catalase, superoxide dismutase, and glutathione reductase are enzymatic salivary antioxidants, and uric acid, reduced glutathione, albumin, and lactoferrin are non-enzymatic antioxidants. Both types of antioxidants regularly donate an electron to free radicals to reduce their levels in cells. Conclusion: Antioxidant activities may be disrupted by signaling mechanisms resulting from barrier regulations observed in tissue. The increased expression of malondialdehyde is an indicator of disrupted antioxidant barriers.
{"title":"Review of salivary antioxidants and their barriers","authors":"Ashlianne Nelson, A. B. Santosh, T. Jones","doi":"10.20473/j.djmkg.v57.i2.p74-79","DOIUrl":"https://doi.org/10.20473/j.djmkg.v57.i2.p74-79","url":null,"abstract":"Background: Oxidative stress results from the imbalance between reactive oxygen species (ROS) and cellular antioxidant defenses. Oxidative stress can cause damage to cellular structures such as cell membranes, lipids, proteins, lipoproteins, and deoxyribonucleic acids when free radicals are present in excess. Antioxidant mechanisms protect tissues against oxidative stress by decreasing free radical levels in cells through the inhibition of activities or expression of free radical-generating enzymes. Purpose: The purpose of this paper is to discuss the role of antioxidants in protecting cells from the harmful effects of ROS, particularly in the context of oral mucosa, and to clarify which molecules disrupt the pathway of these antioxidants. Review: Salivary peroxidase, catalase, superoxide dismutase, and glutathione reductase are enzymatic salivary antioxidants, and uric acid, reduced glutathione, albumin, and lactoferrin are non-enzymatic antioxidants. Both types of antioxidants regularly donate an electron to free radicals to reduce their levels in cells. Conclusion: Antioxidant activities may be disrupted by signaling mechanisms resulting from barrier regulations observed in tissue. The increased expression of malondialdehyde is an indicator of disrupted antioxidant barriers.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"40 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141002202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-06DOI: 10.20473/j.djmkg.v57.i2.p118-123
Annisa Nurul Fikri, Cendrawasih Andusyana Farmasyanti, P. Pudyani
Background: Orthodontic tooth movement (OTM) is a process of tooth movement in the alveolar socket through a bone remodeling process. Cocoa contains caffeine as a bioactive component. The number of studies on the effects of caffeine on orthodontic tooth movement is rising. Purpose: This study aimed to determine the effect of caffeine in cocoa administration on TGF-β1 levels in the pressure side during orthodontic tooth movement. Methods: Twelve Cavia cobaya were divided into 4 groups: control group (ONC), treatment group with 2.3 mg caffeine in cocoa (OWC1), 3.45 mg dose (OWC2) and 4.6 mg dose (OWC3) (n=3). A NiTi open coil spring with light force was applied to two lower incisors tooth of Cavia cobaya. TGF-β1 level in GCF of the pressure side was analyzed using ELISA on days 0, 1, 7, and 14. Data were analyzed using the Two-way ANOVA test (p<0.05) and the LSD Post-Hoc test. Results: Research indicated improvement of TGF-β1 level from the control group with the least average followed group caffeine in cocoa 2.3 mg (OWC1), 3.45 mg (OWC2), and 4.6 mg (OWC3) (p<0.05). Conclusion: This study confirmed that caffeine in cocoa administration increase TGF-β1 level during orthodontics tooth movement on Cavia cobaya in the pressure side.
{"title":"Increased TGF-β1 level after cocoa administration during orthodontics tooth movement in Cavia cobaya","authors":"Annisa Nurul Fikri, Cendrawasih Andusyana Farmasyanti, P. Pudyani","doi":"10.20473/j.djmkg.v57.i2.p118-123","DOIUrl":"https://doi.org/10.20473/j.djmkg.v57.i2.p118-123","url":null,"abstract":"Background: Orthodontic tooth movement (OTM) is a process of tooth movement in the alveolar socket through a bone remodeling process. Cocoa contains caffeine as a bioactive component. The number of studies on the effects of caffeine on orthodontic tooth movement is rising. Purpose: This study aimed to determine the effect of caffeine in cocoa administration on TGF-β1 levels in the pressure side during orthodontic tooth movement. Methods: Twelve Cavia cobaya were divided into 4 groups: control group (ONC), treatment group with 2.3 mg caffeine in cocoa (OWC1), 3.45 mg dose (OWC2) and 4.6 mg dose (OWC3) (n=3). A NiTi open coil spring with light force was applied to two lower incisors tooth of Cavia cobaya. TGF-β1 level in GCF of the pressure side was analyzed using ELISA on days 0, 1, 7, and 14. Data were analyzed using the Two-way ANOVA test (p<0.05) and the LSD Post-Hoc test. Results: Research indicated improvement of TGF-β1 level from the control group with the least average followed group caffeine in cocoa 2.3 mg (OWC1), 3.45 mg (OWC2), and 4.6 mg (OWC3) (p<0.05). Conclusion: This study confirmed that caffeine in cocoa administration increase TGF-β1 level during orthodontics tooth movement on Cavia cobaya in the pressure side.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"54 s56","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141009226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-24DOI: 10.20473/j.djmkg.v56.i4.p280-286
Reza Dony Hendrawan, C. Prahasanti, I. J. Savitri, Padmini Hari
Background: Regenerative periodontal surgical therapy faces significant challenges due to the limited ability of the body to regenerate damaged periodontal tissue. One of the primary goals in regenerative periodontal therapy is regaining periodontal tissue attachment after destruction by periodontal disease. Currently, stem cells, harnessing three pivotal components—cells, biomaterials, and growth factors—are widely used in periodontal regeneration. Stem cells can be obtained from various sources, either by isolating cells from bone marrow, teeth, and muscles or through the somatic cell programming method (reprogramming) known as induced pluripotent stem cells (iPSCs). Purpose: This review aims to describe the potential use of iPSCs in the treatment of periodontal defects. Review: Search strategies were developed using the PubMed, LILACS, Scielo, and Wiley online databases during the period of 2012–2022. Ten articles met the inclusion criteria. iPSCs were obtained by inducing somatic cells from both dental and non-dental sources with factors Oct3/4, Sox2, Klf4, and c-Myc. Periodontal tissue regeneration procedures can be augmented with iPSCs. Unlike tooth-based stem cells, iPSCs offer several advantages, such as unlimited cell sources and the capability to differentiate into any cell type, including periodontal tissue. The potential of iPSCs extends to correcting periodontal bone defects and forming new periodontal tissues, such as alveolar bone, cementum, and periodontal ligament. However, iPSCs do have limitations, including the need for clinical trials, cell programming production facilities, and optimization of differentiated-cell functionality. Conclusion: The combined use of iPSCs in cell-based tissue engineering holds vast potential for future periodontal treatment strategies.
{"title":"Induced pluripotent stem cells in periodontal reconstructive therapy: A narrative review of pre-clinical studies","authors":"Reza Dony Hendrawan, C. Prahasanti, I. J. Savitri, Padmini Hari","doi":"10.20473/j.djmkg.v56.i4.p280-286","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i4.p280-286","url":null,"abstract":"Background: Regenerative periodontal surgical therapy faces significant challenges due to the limited ability of the body to regenerate damaged periodontal tissue. One of the primary goals in regenerative periodontal therapy is regaining periodontal tissue attachment after destruction by periodontal disease. Currently, stem cells, harnessing three pivotal components—cells, biomaterials, and growth factors—are widely used in periodontal regeneration. Stem cells can be obtained from various sources, either by isolating cells from bone marrow, teeth, and muscles or through the somatic cell programming method (reprogramming) known as induced pluripotent stem cells (iPSCs). Purpose: This review aims to describe the potential use of iPSCs in the treatment of periodontal defects. Review: Search strategies were developed using the PubMed, LILACS, Scielo, and Wiley online databases during the period of 2012–2022. Ten articles met the inclusion criteria. iPSCs were obtained by inducing somatic cells from both dental and non-dental sources with factors Oct3/4, Sox2, Klf4, and c-Myc. Periodontal tissue regeneration procedures can be augmented with iPSCs. Unlike tooth-based stem cells, iPSCs offer several advantages, such as unlimited cell sources and the capability to differentiate into any cell type, including periodontal tissue. The potential of iPSCs extends to correcting periodontal bone defects and forming new periodontal tissues, such as alveolar bone, cementum, and periodontal ligament. However, iPSCs do have limitations, including the need for clinical trials, cell programming production facilities, and optimization of differentiated-cell functionality. Conclusion: The combined use of iPSCs in cell-based tissue engineering holds vast potential for future periodontal treatment strategies.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"277 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139314582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-13DOI: 10.20473/j.djmkg.v56.i3.p202-207
Cokorda Gde Suryabharata, Andra Rizqiawan, I. Mulyawan, S. Wati, M. Z. Rahman
Background: A dentigerous cyst is the second-most frequently observed odontogenic cyst, and an ameloblastoma is one of the most frequently observed odontogenic tumors. Both are mostly associated with an impacted mandibular third molar and have similar characteristics. Diagnostic difficulties often result in misdiagnosis and remain a challenge to overcome. Comprehensive clinical, radiographic, and histopathological views are essential to correctly diagnose the problem and formulate the most suitable treatment plan. Purpose: This case report aims to present a thorough approach to the diagnostic and surgical procedures involved in treating a dentigerous cyst that resembles an ameloblastoma by using marsupialization followed by enucleation. Case: This article presents the case report of a 27-year-old male patient with an infected dentigerous cyst resembling a unicystic ameloblastoma associated with a totally impacted lower mandibular left third molar. The patient had a history of swelling, pain, and pus drainage in the retromolar area. Symptoms subsided after antibiotic and analgesic prescriptions, but the lesion remained and was slowly progressing. The diagnostic approach began with a fine-needle aspiration biopsy, and the result confirmed a benign cystic lesion that was suspected to be an odontogenic tumor. Then an incisional biopsy was conducted under local anesthesia, diagnosing an infected dentigerous cyst. Case Management: Marsupialization and decompression using an obturator was preferred, followed by enucleation. Nine months later, a radiographic examination revealed satisfactory bone regeneration without recurrence. Conclusion: An incisional biopsy plays a vital role in establishing a definitive diagnosis. Marsupialization followed by enucleation offers an excellent combination of treatments achieving complete cyst removal, anatomical structure preservation, and bone regeneration with minimal complications.
{"title":"The diagnostic challenges and two-step surgical approach to an infected dentigerous cyst resembling a unicystic ameloblastoma: A case report","authors":"Cokorda Gde Suryabharata, Andra Rizqiawan, I. Mulyawan, S. Wati, M. Z. Rahman","doi":"10.20473/j.djmkg.v56.i3.p202-207","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p202-207","url":null,"abstract":"Background: A dentigerous cyst is the second-most frequently observed odontogenic cyst, and an ameloblastoma is one of the most frequently observed odontogenic tumors. Both are mostly associated with an impacted mandibular third molar and have similar characteristics. Diagnostic difficulties often result in misdiagnosis and remain a challenge to overcome. Comprehensive clinical, radiographic, and histopathological views are essential to correctly diagnose the problem and formulate the most suitable treatment plan. Purpose: This case report aims to present a thorough approach to the diagnostic and surgical procedures involved in treating a dentigerous cyst that resembles an ameloblastoma by using marsupialization followed by enucleation. Case: This article presents the case report of a 27-year-old male patient with an infected dentigerous cyst resembling a unicystic ameloblastoma associated with a totally impacted lower mandibular left third molar. The patient had a history of swelling, pain, and pus drainage in the retromolar area. Symptoms subsided after antibiotic and analgesic prescriptions, but the lesion remained and was slowly progressing. The diagnostic approach began with a fine-needle aspiration biopsy, and the result confirmed a benign cystic lesion that was suspected to be an odontogenic tumor. Then an incisional biopsy was conducted under local anesthesia, diagnosing an infected dentigerous cyst. Case Management: Marsupialization and decompression using an obturator was preferred, followed by enucleation. Nine months later, a radiographic examination revealed satisfactory bone regeneration without recurrence. Conclusion: An incisional biopsy plays a vital role in establishing a definitive diagnosis. Marsupialization followed by enucleation offers an excellent combination of treatments achieving complete cyst removal, anatomical structure preservation, and bone regeneration with minimal complications.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45893517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-13DOI: 10.20473/j.djmkg.v56.i3.p139-143
Mehmet Veysel Kotanli, M. Doğan, Sedef Kotanli, M. Doğan
Background: Although dental trauma has been reported at various frequencies worldwide, as far as we know there are few data regarding the frequency of dental trauma during the pandemic period. Purpose: This study aims to retrospectively evaluate the data of pediatric patients who were admitted to the Department of Pedodontics in a university hospital due to dental trauma during the COVID-19 pandemic. Methods: Between April 2020 and December 2020, the clinical records regarding admissions to the Harran University Faculty of Dentistry Pedodontics clinic due to dental trauma were examined. The age and gender of the patient, type of trauma, teeth affected by the trauma, and treatment methods applied after the trauma were recorded. The data obtained were analyzed using the independent sample t-test for intergroup comparisons and Pearson chi-square test for categorical variables with the help of Statistical Package for the Social Sciences version 23.0. Results: The data of 43 patients (27 males and 16 females) aged between 1–13 years (mean age: 9.13±3.20) who were admitted to the clinic with trauma complaints during an eight-month period were evaluated. It was determined that the most common trauma types in the pandemic period were simple crown fracture (25.6%) and subluxation/lateral luxation (23.3%). During the pandemic, 34.9% of all cases were “falling at home”, while “falling at school” was 14.0% (using the Pearson chi-square test). Conclusion: The COVID-19 pandemic period has affected many aspects of social life as well as the number of patients who were admitted for dental trauma and the causes of trauma. Multicenter studies are needed for more accurate results.
背景:尽管在世界范围内报告的牙外伤频率各不相同，但据我们所知，关于大流行期间牙外伤频率的数据很少。目的:回顾性分析2019冠状病毒病大流行期间因牙外伤入住某大学附属医院儿科的患儿资料。方法:对2020年4月至2020年12月期间因牙外伤就诊的哈兰大学牙科学院儿科学门诊的临床记录进行分析。记录患者的年龄、性别、创伤类型、受创伤的牙齿以及创伤后的治疗方法。使用Statistical Package for The Social Sciences version 23.0对所得数据进行组间比较的独立样本t检验和分类变量的皮尔逊卡方检验。结果:对我院收治的43例(男27例，女16例)年龄1 ~ 13岁(平均年龄:9.13±3.20岁)8个月的创伤主诉患者资料进行分析。结果表明，大流行期间最常见的创伤类型为单纯冠状骨折(25.6%)和半脱位/外侧脱位(23.3%)。在大流行期间，34.9%的病例“在家跌倒”，14.0%的病例“在学校跌倒”(使用Pearson卡方检验)。结论:2019冠状病毒病大流行期对社会生活的诸多方面产生了影响，同时也影响了因牙外伤入院的患者数量和外伤的原因。需要多中心研究才能获得更准确的结果。
{"title":"Dental traumatic injuries during the COVID-19 pandemic: A retrospective study","authors":"Mehmet Veysel Kotanli, M. Doğan, Sedef Kotanli, M. Doğan","doi":"10.20473/j.djmkg.v56.i3.p139-143","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p139-143","url":null,"abstract":"Background: Although dental trauma has been reported at various frequencies worldwide, as far as we know there are few data regarding the frequency of dental trauma during the pandemic period. Purpose: This study aims to retrospectively evaluate the data of pediatric patients who were admitted to the Department of Pedodontics in a university hospital due to dental trauma during the COVID-19 pandemic. Methods: Between April 2020 and December 2020, the clinical records regarding admissions to the Harran University Faculty of Dentistry Pedodontics clinic due to dental trauma were examined. The age and gender of the patient, type of trauma, teeth affected by the trauma, and treatment methods applied after the trauma were recorded. The data obtained were analyzed using the independent sample t-test for intergroup comparisons and Pearson chi-square test for categorical variables with the help of Statistical Package for the Social Sciences version 23.0. Results: The data of 43 patients (27 males and 16 females) aged between 1–13 years (mean age: 9.13±3.20) who were admitted to the clinic with trauma complaints during an eight-month period were evaluated. It was determined that the most common trauma types in the pandemic period were simple crown fracture (25.6%) and subluxation/lateral luxation (23.3%). During the pandemic, 34.9% of all cases were “falling at home”, while “falling at school” was 14.0% (using the Pearson chi-square test). Conclusion: The COVID-19 pandemic period has affected many aspects of social life as well as the number of patients who were admitted for dental trauma and the causes of trauma. Multicenter studies are needed for more accurate results.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45713072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p166-171
Sandy Christiono, Seno Pradopo, I. Sudiana, I. Hutami, Regilia Shinta Mayangsari, Y. S. Rochmah, Zurairah Ibrahim
Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.
{"title":"Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus)","authors":"Sandy Christiono, Seno Pradopo, I. Sudiana, I. Hutami, Regilia Shinta Mayangsari, Y. S. Rochmah, Zurairah Ibrahim","doi":"10.20473/j.djmkg.v56.i3.p166-171","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p166-171","url":null,"abstract":"Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46178603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p172-177
Titien Hary Agustantina, E. Munadziroh, A. Yuliati, Muhammad Riza Hafidz Bahtiar, Octarina, R. F. Salma, Ajeng Putri Meyranti, F. A. Razak
Background: A good biocomposite is a structure that can provide opportunities for cells to adhere, proliferate, and differentiate. It is affected by the characteristics of a material. As bone tissue regeneration occurs, biomaterials must have a high swelling ability and low biodegradability. The high swelling capability will have a larger surface area that can support maximal cell attachment and proliferation on the biocomposite surface, which accelerates the regeneration process of bone defects. Purpose: The study aimed to analyze the characteristics of swelling and biodegradation of bovine amniotic membrane-hydroxyapatite (BAM-HA) biocomposite with various ratios. Methods: The BAM-HA biocomposite with a ratio of 30:70, 35:65, and 40:60 (w/w) was synthesized using a freeze-dry method. The swelling test was done by measuring the initial weight and final weight after being soaked in phosphate-buffered saline for 24 hours and the biodegradation test was done by measuring the initial weight and final weight after being soaked in simulated body fluid for seven days. Results: The swelling percentage of BAM-HA biocomposite at each ratio of 30:70, 35:65, and 40:60 (w/w) was 303.90%, 477.94%, and 574.19%. The biodegradation percentage of BAM-HA biocomposite at each ratio of 30:70, 35:65, and 40:60 was 9.43%, 11.05%, and 12.02%. Conclusion: The BAM-HA biocomposite with a ratio of 40:60 (w/w) has the highest swelling percentage while the 30:70 (w/w) ratio has the lowest percentage of biodegradation.
{"title":"The characteristics of swelling and biodegradation tests of bovine amniotic membrane-hydroxyapatite biocomposite","authors":"Titien Hary Agustantina, E. Munadziroh, A. Yuliati, Muhammad Riza Hafidz Bahtiar, Octarina, R. F. Salma, Ajeng Putri Meyranti, F. A. Razak","doi":"10.20473/j.djmkg.v56.i3.p172-177","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p172-177","url":null,"abstract":"Background: A good biocomposite is a structure that can provide opportunities for cells to adhere, proliferate, and differentiate. It is affected by the characteristics of a material. As bone tissue regeneration occurs, biomaterials must have a high swelling ability and low biodegradability. The high swelling capability will have a larger surface area that can support maximal cell attachment and proliferation on the biocomposite surface, which accelerates the regeneration process of bone defects. Purpose: The study aimed to analyze the characteristics of swelling and biodegradation of bovine amniotic membrane-hydroxyapatite (BAM-HA) biocomposite with various ratios. Methods: The BAM-HA biocomposite with a ratio of 30:70, 35:65, and 40:60 (w/w) was synthesized using a freeze-dry method. The swelling test was done by measuring the initial weight and final weight after being soaked in phosphate-buffered saline for 24 hours and the biodegradation test was done by measuring the initial weight and final weight after being soaked in simulated body fluid for seven days. Results: The swelling percentage of BAM-HA biocomposite at each ratio of 30:70, 35:65, and 40:60 (w/w) was 303.90%, 477.94%, and 574.19%. The biodegradation percentage of BAM-HA biocomposite at each ratio of 30:70, 35:65, and 40:60 was 9.43%, 11.05%, and 12.02%. Conclusion: The BAM-HA biocomposite with a ratio of 40:60 (w/w) has the highest swelling percentage while the 30:70 (w/w) ratio has the lowest percentage of biodegradation.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49025640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p197-201
Eddy Hermanto, F. M. Laihad, Amelia Elizabeth Pranoto, Monika Elidasari, Ivan Tantra, Sarianoferni, Dian Widya Damayanti
Background: Jaw fracture is the most common facial fracture in oral and maxillofacial bone and is usually caused by trauma. The fracture itself could lead to infection due to bone and tissue damage, which is the port of entry for microorganisms. Fracture-related infection (FRI) in the patient discussed in this study manifested as a submandibular abscess. The goals of fracture treatment were achieving the anatomic reduction of the fracture line and regaining acceptable occlusion. There are two methods for treating mandibular fractures: the closed method, also called conservative treatment, and the open method, which requires advanced surgery. Closed method treatment uses a maxillomandibular fixation (MMF) device in order to reduce and immobilize fracture fragments. Treatment of FRI should use a multidisciplinary approach to achieve an outstanding result, such as wound debridement, antimicrobial therapy, and implant retention. Purpose: The purpose of this article is to report a case of neglected mandibular fracture with a submandibular abscess, which was treated with a combination of the closed reduction method and incision drainage. Case: A 25-year-old female visited Nala Husada Hospital because of a submandibular abscess on the neglected mandibular fracture of the right parasymphysis and left corpus. Case management: The case was managed using an arch bar in the mandible and an eyelet in the maxilla while continuing with MMF and an extra oral drainage incision. Conclusion: Combination therapy (MMF and incision drainage) was needed to treat this case because of the occurrence of a submandibular abscess due to a neglected mandibular fracture.
{"title":"Closed reduction and drainage incision for the treatment of neglected and infected mandibular fractures","authors":"Eddy Hermanto, F. M. Laihad, Amelia Elizabeth Pranoto, Monika Elidasari, Ivan Tantra, Sarianoferni, Dian Widya Damayanti","doi":"10.20473/j.djmkg.v56.i3.p197-201","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p197-201","url":null,"abstract":"Background: Jaw fracture is the most common facial fracture in oral and maxillofacial bone and is usually caused by trauma. The fracture itself could lead to infection due to bone and tissue damage, which is the port of entry for microorganisms. Fracture-related infection (FRI) in the patient discussed in this study manifested as a submandibular abscess. The goals of fracture treatment were achieving the anatomic reduction of the fracture line and regaining acceptable occlusion. There are two methods for treating mandibular fractures: the closed method, also called conservative treatment, and the open method, which requires advanced surgery. Closed method treatment uses a maxillomandibular fixation (MMF) device in order to reduce and immobilize fracture fragments. Treatment of FRI should use a multidisciplinary approach to achieve an outstanding result, such as wound debridement, antimicrobial therapy, and implant retention. Purpose: The purpose of this article is to report a case of neglected mandibular fracture with a submandibular abscess, which was treated with a combination of the closed reduction method and incision drainage. Case: A 25-year-old female visited Nala Husada Hospital because of a submandibular abscess on the neglected mandibular fracture of the right parasymphysis and left corpus. Case management: The case was managed using an arch bar in the mandible and an eyelet in the maxilla while continuing with MMF and an extra oral drainage incision. Conclusion: Combination therapy (MMF and incision drainage) was needed to treat this case because of the occurrence of a submandibular abscess due to a neglected mandibular fracture.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45068519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p160-165
Devi Rianti, Alqomariyah Eka Purnamasari, Rifayinqa Ruyani Putri, Noor Zain Salsabilla, Faradillah, E. Munadziroh, Titien Hary Agustantina, Asti Meizarini, A. Yuliati, A. Syahrom
Background: One of the main components in tissue engineering is the scaffold, which may serve as a medium to support cell and tissue growth. Scaffolds must have good compressive strength and controlled biodegradability to show biological activities while treating bone defects. This study uses Chitosan-gelatin (C–G) with good flexibility and elasticity and high-strength carbonate hydroxyapatite (CHA), which may be the ideal scaffold for tissue engineering. Purpose: To analyze the compressive strength and static biodegradation rate within various ratios of C–G and CHA (C–G:CHA) scaffold as a requirement for bone tissue engineering. Methods: The scaffold is synthesized from C–G:CHA with three ratio variations, which are 40:60, 30:70, and 20:80 (weight for weight [w/w]), made with a freeze-drying method. The compressive strengths are then tested. The biodegradation rate is tested by soaking the scaffold in simulated body fluid for 1, 3, 7, 14, and 21 days. Data are analyzed with a one-way ANOVA parametric test. Results: The compressive strength of each ratio of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w), consecutively, are 4.2 Megapascals (MPa), 3.3 MPa, 2.2 MPa, and there are no significant differences with the p= 0.069 (p>0.05). The static biodegradation percentage after 21 days on each ratio variation of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w) is 25.98%, 24.67%, and 20.64%. One-way ANOVA Welch test shows the result of the p-value as p<0.05. Conclusion: The compressive strength and static biodegradation of the C–G:CHA scaffold with ratio variations of 40:60 (w/w), 30:70 (w/w), and 20:80(w/w) fulfilled the requirements as a scaffold for bone tissue engineering.
{"title":"The compressive strength and static biodegradation rate of chitosan-gelatin limestone-based carbonate hydroxyapatite composite scaffold","authors":"Devi Rianti, Alqomariyah Eka Purnamasari, Rifayinqa Ruyani Putri, Noor Zain Salsabilla, Faradillah, E. Munadziroh, Titien Hary Agustantina, Asti Meizarini, A. Yuliati, A. Syahrom","doi":"10.20473/j.djmkg.v56.i3.p160-165","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p160-165","url":null,"abstract":"Background: One of the main components in tissue engineering is the scaffold, which may serve as a medium to support cell and tissue growth. Scaffolds must have good compressive strength and controlled biodegradability to show biological activities while treating bone defects. This study uses Chitosan-gelatin (C–G) with good flexibility and elasticity and high-strength carbonate hydroxyapatite (CHA), which may be the ideal scaffold for tissue engineering. Purpose: To analyze the compressive strength and static biodegradation rate within various ratios of C–G and CHA (C–G:CHA) scaffold as a requirement for bone tissue engineering. Methods: The scaffold is synthesized from C–G:CHA with three ratio variations, which are 40:60, 30:70, and 20:80 (weight for weight [w/w]), made with a freeze-drying method. The compressive strengths are then tested. The biodegradation rate is tested by soaking the scaffold in simulated body fluid for 1, 3, 7, 14, and 21 days. Data are analyzed with a one-way ANOVA parametric test. Results: The compressive strength of each ratio of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w), consecutively, are 4.2 Megapascals (MPa), 3.3 MPa, 2.2 MPa, and there are no significant differences with the p= 0.069 (p>0.05). The static biodegradation percentage after 21 days on each ratio variation of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w) is 25.98%, 24.67%, and 20.64%. One-way ANOVA Welch test shows the result of the p-value as p<0.05. Conclusion: The compressive strength and static biodegradation of the C–G:CHA scaffold with ratio variations of 40:60 (w/w), 30:70 (w/w), and 20:80(w/w) fulfilled the requirements as a scaffold for bone tissue engineering.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46778033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p189-196
Karlina Puspasari, T. A. S. Pasaribu, M. Surboyo, N. Ayuningtyas, A. B. Santosh, D. Ernawati
Background: Oral cancer therapy, such as radiation or surgical treatment, has pernicious long-term effects that patients suffer throughout their life, the disability being considerable with delayed diagnosis. It is well known that many oral cancers develop from oral potentially malignant disorders (OPMDs). Patients diagnosed with OPMDs may have an increased risk of developing cancer anywhere in the oral cavity. Early detection and intervention could be essential prevention strategies to inhibit oral cancer progression. OPMDs may not immediately develop into carcinoma. However, this condition provides a “field” of specific abnormalities wherein evolving altered genetic cells can be explained with the “field cancerization” concept. Purpose: This review aims to describe the “field cancerization” concept in oral cancer and OPMD, which is expected to contribute to a better clinical management strategy for oral cancer prevention. Review: “Oral field cancerization” describes oral cancers that develop in multifocal areas of pre-cancerous changes. It can be found as histologically abnormal tissue surrounding the tumor, suggesting that oral cancer often consists of multiple independent lesions. Conclusion: The oral field cancerization concept should prompt healthcare professionals to remind their patients that frequent oral examination with histological studies and molecular testing is mandatory for those at high risk of developing malignancies.
{"title":"Oral field cancerization: Genetic profiling for a prevention strategy for oral potentially malignant disorders","authors":"Karlina Puspasari, T. A. S. Pasaribu, M. Surboyo, N. Ayuningtyas, A. B. Santosh, D. Ernawati","doi":"10.20473/j.djmkg.v56.i3.p189-196","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p189-196","url":null,"abstract":"Background: Oral cancer therapy, such as radiation or surgical treatment, has pernicious long-term effects that patients suffer throughout their life, the disability being considerable with delayed diagnosis. It is well known that many oral cancers develop from oral potentially malignant disorders (OPMDs). Patients diagnosed with OPMDs may have an increased risk of developing cancer anywhere in the oral cavity. Early detection and intervention could be essential prevention strategies to inhibit oral cancer progression. OPMDs may not immediately develop into carcinoma. However, this condition provides a “field” of specific abnormalities wherein evolving altered genetic cells can be explained with the “field cancerization” concept. Purpose: This review aims to describe the “field cancerization” concept in oral cancer and OPMD, which is expected to contribute to a better clinical management strategy for oral cancer prevention. Review: “Oral field cancerization” describes oral cancers that develop in multifocal areas of pre-cancerous changes. It can be found as histologically abnormal tissue surrounding the tumor, suggesting that oral cancer often consists of multiple independent lesions. Conclusion: The oral field cancerization concept should prompt healthcare professionals to remind their patients that frequent oral examination with histological studies and molecular testing is mandatory for those at high risk of developing malignancies.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41602708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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