Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p144-153
Sri Nabawiyati Nurul Makiyah, Sartika Puspita
Background: The trend of studies on dental medicaments is increasing rapidly. Antibacterial or anti-inflammatory activity is most frequently studied. Ovalbumin is one of the proteins whose benefits have been studied, but these benefits are still limited because of ovalbumin’s potential for proliferative bioactivity. Purpose: The aim of this study is to examine ovalbumin’s potential as a woundhealing medicament through molecular docking analysis on a protein related to the extracellular signal-regulated kinases/mitogenactivated protein kinase (ERK/MAPK) signaling pathway. Methods: Ovalbumin was hydrolyzed through BIOPEP-UWM (The BIOPEPUWM™ database of bioactive peptides). Protein target and interaction were predicted using Similarity Ensemble Approach target prediction webserver, SuperPred webserver, STRING webserver, and Cytoscape version 3.9.1. Selected fragments were docked using Autodock Vina in PyRx 0.8 with Tukey’s multiple comparison test and Biovia Discovery Studio version 19.1.0.18287 for visualization. Results: This study found that ovalbumin has the potential to positively regulate cell proliferation, angiogenesis, and fibroblast growth factor production. Six of the 131 fragments of ovalbumin could interact with 73 proteins, and the 20 proteins with the highest probability and score of betweenness centrality showed potential for bioactivity. Five fragments and povidone-iodine interacted inside the Adenosine triphosphate (ATP) phosphorylation site of ERK2, whereas fragment 1 (F1) and glycerin interacted outside the site. F1 could decrease the binding energy required for adenosine 5′-[,-methylene]triphosphate or an ATP-analogue chemical compound to interact with ERK2 compared to the control, with a score that was not significant. Conclusion: Ovalbumin has the potential to induce cell proliferation by affecting ERK2-ligand interactions.
{"title":"Ovalbumin’s potential as a wound-healing medicament in tooth extraction socket by induction of cell proliferation through the ERK2 pathway in silico","authors":"Sri Nabawiyati Nurul Makiyah, Sartika Puspita","doi":"10.20473/j.djmkg.v56.i3.p144-153","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p144-153","url":null,"abstract":"Background: The trend of studies on dental medicaments is increasing rapidly. Antibacterial or anti-inflammatory activity is most frequently studied. Ovalbumin is one of the proteins whose benefits have been studied, but these benefits are still limited because of ovalbumin’s potential for proliferative bioactivity. Purpose: The aim of this study is to examine ovalbumin’s potential as a woundhealing medicament through molecular docking analysis on a protein related to the extracellular signal-regulated kinases/mitogenactivated protein kinase (ERK/MAPK) signaling pathway. Methods: Ovalbumin was hydrolyzed through BIOPEP-UWM (The BIOPEPUWM™ database of bioactive peptides). Protein target and interaction were predicted using Similarity Ensemble Approach target prediction webserver, SuperPred webserver, STRING webserver, and Cytoscape version 3.9.1. Selected fragments were docked using Autodock Vina in PyRx 0.8 with Tukey’s multiple comparison test and Biovia Discovery Studio version 19.1.0.18287 for visualization. Results: This study found that ovalbumin has the potential to positively regulate cell proliferation, angiogenesis, and fibroblast growth factor production. Six of the 131 fragments of ovalbumin could interact with 73 proteins, and the 20 proteins with the highest probability and score of betweenness centrality showed potential for bioactivity. Five fragments and povidone-iodine interacted inside the Adenosine triphosphate (ATP) phosphorylation site of ERK2, whereas fragment 1 (F1) and glycerin interacted outside the site. F1 could decrease the binding energy required for adenosine 5′-[,-methylene]triphosphate or an ATP-analogue chemical compound to interact with ERK2 compared to the control, with a score that was not significant. Conclusion: Ovalbumin has the potential to induce cell proliferation by affecting ERK2-ligand interactions.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41920554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The cause of cavities is initially due to roughness on the tooth surface, requiring fluoride varnish to prevent caries, as the varnish applies a fluoride compound to the tooth surface. Fluoride varnish reacts with the tooth enamel surface to form calcium fluoride and fluorapatite, thus making the enamel surface more resistant to demineralization and damage. Purpose: This study aims to compare the roughness of tooth enamel surfaces among three fluoride varnishes under acidic conditions. Methods: The research method uses three fluoride varnish materials: sodium fluoride 5% + tricalcium phosphate, calcium fluoride, and sodium fluoride 5% + casein phosphopeptide-amorphous calcium phosphate 2%. Samples of 81 teeth were divided into three groups (Group 1 without fluoride varnish application, Group 2 application of fluoride varnish with pH 3, and Group 3 application of fluoride varnish with pH 5). The teeth were tested before and after application of the varnishes using the scanning electron microscope and surface roughness tests. Results: The results showed a significant difference in the mean surface roughness of the enamel of the anterior deciduous teeth tested with fluoride varnish. The before and after comparisons in the pH 3 and pH 5 groups were very significant (p-value 0.000). The comparison results in each pH group after fluoride varnish administration showed no significant difference (pH 3 p-value 0.074 and pH 5 p-value 0.196). The tooth surfaces appear to be rougher after administration of an all-acid solution. Conclusion: There is a difference in surface roughness of primary teeth after being given fluoride varnish in low pH 3 immersion for 24 hours.
{"title":"Surface roughness assessment with fluoride varnish application: An in vitro study","authors":"Anie Apriani, Silvia Naliani, Rudy Djuanda, Shania Hysan Teanindar, Jessica Quiteria Florenthe, Ferri Baharudin","doi":"10.20473/j.djmkg.v56.i3.p154-159","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p154-159","url":null,"abstract":"Background: The cause of cavities is initially due to roughness on the tooth surface, requiring fluoride varnish to prevent caries, as the varnish applies a fluoride compound to the tooth surface. Fluoride varnish reacts with the tooth enamel surface to form calcium fluoride and fluorapatite, thus making the enamel surface more resistant to demineralization and damage. Purpose: This study aims to compare the roughness of tooth enamel surfaces among three fluoride varnishes under acidic conditions. Methods: The research method uses three fluoride varnish materials: sodium fluoride 5% + tricalcium phosphate, calcium fluoride, and sodium fluoride 5% + casein phosphopeptide-amorphous calcium phosphate 2%. Samples of 81 teeth were divided into three groups (Group 1 without fluoride varnish application, Group 2 application of fluoride varnish with pH 3, and Group 3 application of fluoride varnish with pH 5). The teeth were tested before and after application of the varnishes using the scanning electron microscope and surface roughness tests. Results: The results showed a significant difference in the mean surface roughness of the enamel of the anterior deciduous teeth tested with fluoride varnish. The before and after comparisons in the pH 3 and pH 5 groups were very significant (p-value 0.000). The comparison results in each pH group after fluoride varnish administration showed no significant difference (pH 3 p-value 0.074 and pH 5 p-value 0.196). The tooth surfaces appear to be rougher after administration of an all-acid solution. Conclusion: There is a difference in surface roughness of primary teeth after being given fluoride varnish in low pH 3 immersion for 24 hours.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49405665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p208-212
Aris Setyawan, Zhavira Dwiyanti Anang, Rizki Nurida Rahmawati, Winda Kumalasari, Alfi Dian Uly Noor, R. Yulianti
Background: Oral squamous cell carcinoma (OSCC) occurs in approximately 95% of people older than 40 years; usually, it is diagnosed at the age of 60 years. The etiology of OSCC is multifactorial. Chronic mechanical trauma due to sharp teeth has also been suggested as an etiology of oral squamous of the tongue. Purpose: This case report aimed to describe the etiology of OSCC resulting from mechanical trauma and to prevent further metastasis by correctly diagnosing the lesion earlier. Case: A 49-year-old female patient presented with painful and swollen stomatitis two months ago. No submandibular lymph nodes were palpable. An indurated ulceration and sharp teeth on the right region of the jaw were obtained from the intraoral examination. Malignancy was concluded from Histopathology Examination (HPE) and Magnetic Resonance Imaging (MRI) radiography results. Case Management: Multiple extractions were chosen to eliminate the etiology of mechanical trauma due to sharp teeth, and the patient underwent hemiglossectomy with the keyhole method. Postoperative, the histopathology examination revealed an OSCC of the tongue. The patient is currently in the second cycle of chemotherapy by hematologist-oncologists. Conclusion: The early appropriate diagnosis of a tongue ulcer can prevent metastasis, decrease morbidity, and increase quality of life.
{"title":"The early detection of tongue cancer with the etiology of mechanical trauma","authors":"Aris Setyawan, Zhavira Dwiyanti Anang, Rizki Nurida Rahmawati, Winda Kumalasari, Alfi Dian Uly Noor, R. Yulianti","doi":"10.20473/j.djmkg.v56.i3.p208-212","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p208-212","url":null,"abstract":"Background: Oral squamous cell carcinoma (OSCC) occurs in approximately 95% of people older than 40 years; usually, it is diagnosed at the age of 60 years. The etiology of OSCC is multifactorial. Chronic mechanical trauma due to sharp teeth has also been suggested as an etiology of oral squamous of the tongue. Purpose: This case report aimed to describe the etiology of OSCC resulting from mechanical trauma and to prevent further metastasis by correctly diagnosing the lesion earlier. Case: A 49-year-old female patient presented with painful and swollen stomatitis two months ago. No submandibular lymph nodes were palpable. An indurated ulceration and sharp teeth on the right region of the jaw were obtained from the intraoral examination. Malignancy was concluded from Histopathology Examination (HPE) and Magnetic Resonance Imaging (MRI) radiography results. Case Management: Multiple extractions were chosen to eliminate the etiology of mechanical trauma due to sharp teeth, and the patient underwent hemiglossectomy with the keyhole method. Postoperative, the histopathology examination revealed an OSCC of the tongue. The patient is currently in the second cycle of chemotherapy by hematologist-oncologists. Conclusion: The early appropriate diagnosis of a tongue ulcer can prevent metastasis, decrease morbidity, and increase quality of life.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47432955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p184-188
Ega Lucida Chandra Kumala, Malianawati Fauzia, Hana Salsabila Junivianti
Background: The use of a bone graft in bone regeneration is challenging. Tooth graft material has been used as a bone graft alternative due to its similar composition of organic and inorganic materials close to the bone. Recently, nanotechnology has been used to improve bone graft quality. The osteoconduction rate in the defect area represents the bone graft quality. Purpose: This study aimed to compare the number of osteoblasts using nano-tooth grafts and micro-tooth grafts in Wistar rats. Methods: Wistar rats were divided into six groups: the negative control groups (examined on days 7 and 14), the micro-tooth graft groups (examined on days 7 and 14), and the nano-tooth graft groups (examined on days 7 and 14). The control group received nothing, the micro-tooth group received a micro-size tooth graft, and the nano-tooth graft group received a nano-size tooth graft on the injured femur. Histological observations of osteoblasts were carried out using a light microscope with 1000x magnification. Data were analyzed using one-way analysis of variance and least significant difference tests. Results: On day 7, the nano-tooth graft group showed a higher osteoblast number (11.75) than the micro-tooth graft group (7.5) (p = 0.039). There was no significant difference in the micro-tooth graft group compared to the control (p > 0.05). On day 14, the nano-tooth graft group showed a decrease in osteoblast number close to normal (control) (p > 0.05), while the micro-tooth graft group still experienced significant elevation. Conclusion: Nano-tooth grafts accelerate the stimulation of osteoblasts in the first stages of the healing process compared to micro-tooth grafts.
{"title":"The effect of nanoparticle tooth grafts on osteoblast stimulation in the first stages of the bone healing process in Wistar rats compared to the micro-tooth graft technique","authors":"Ega Lucida Chandra Kumala, Malianawati Fauzia, Hana Salsabila Junivianti","doi":"10.20473/j.djmkg.v56.i3.p184-188","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p184-188","url":null,"abstract":"Background: The use of a bone graft in bone regeneration is challenging. Tooth graft material has been used as a bone graft alternative due to its similar composition of organic and inorganic materials close to the bone. Recently, nanotechnology has been used to improve bone graft quality. The osteoconduction rate in the defect area represents the bone graft quality. Purpose: This study aimed to compare the number of osteoblasts using nano-tooth grafts and micro-tooth grafts in Wistar rats. Methods: Wistar rats were divided into six groups: the negative control groups (examined on days 7 and 14), the micro-tooth graft groups (examined on days 7 and 14), and the nano-tooth graft groups (examined on days 7 and 14). The control group received nothing, the micro-tooth group received a micro-size tooth graft, and the nano-tooth graft group received a nano-size tooth graft on the injured femur. Histological observations of osteoblasts were carried out using a light microscope with 1000x magnification. Data were analyzed using one-way analysis of variance and least significant difference tests. Results: On day 7, the nano-tooth graft group showed a higher osteoblast number (11.75) than the micro-tooth graft group (7.5) (p = 0.039). There was no significant difference in the micro-tooth graft group compared to the control (p > 0.05). On day 14, the nano-tooth graft group showed a decrease in osteoblast number close to normal (control) (p > 0.05), while the micro-tooth graft group still experienced significant elevation. Conclusion: Nano-tooth grafts accelerate the stimulation of osteoblasts in the first stages of the healing process compared to micro-tooth grafts.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42964317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.20473/j.djmkg.v56.i3.p178-183
M. L. Apriasari, Tiara Intan Permata, Wayan Arya, Krishnawan Firdaus
Background: Kalimantan’s people consume Stenochlaena palustris leaf extract as food and for traditional medicine. The bioactive components of Stenochlaena palustris leaf extract are flavonoids, alkaloids, saponins, and tannins. An in vitro study shows that the leaf extract has no toxic effect, so it can be used as an alternative drug in oral health, such as in mouthwashes or topical ulcer drugs. Purpose: This study aims to analyze the toxic effects of Stenochlaena palustris leaf extract based on the bleeding and lesions resulting from necrosis in kidney by using histopathology examination. Methods: The Stenochlaena palustris leaves were extracted using 95% ethanol and then given to male Wistar strain (Rattus norvegicus) with a 2,000, 2,500, and 3,000 mg/kg/body weight two times a day for fourteen days. The kidneys were collected and subjected to histopathology examination. Results: There are higher bleeding and necrosis lesion rates in the 2,500 and 3,000 mg/kg/body weight of Stenochlaena palustris leaves extract group compared to the control and 2,000 mg/kg/body weight of Stenochlaena palustris leaves extract group (p<0.05). Conclusion: Stenochlaena palustris leaf extract showed no toxic effect at doses of 2,000 mg/kg/body weight.
{"title":"Toxicity test of Stenochlaena palustris extract based on kidney histopathology examination","authors":"M. L. Apriasari, Tiara Intan Permata, Wayan Arya, Krishnawan Firdaus","doi":"10.20473/j.djmkg.v56.i3.p178-183","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i3.p178-183","url":null,"abstract":"Background: Kalimantan’s people consume Stenochlaena palustris leaf extract as food and for traditional medicine. The bioactive components of Stenochlaena palustris leaf extract are flavonoids, alkaloids, saponins, and tannins. An in vitro study shows that the leaf extract has no toxic effect, so it can be used as an alternative drug in oral health, such as in mouthwashes or topical ulcer drugs. Purpose: This study aims to analyze the toxic effects of Stenochlaena palustris leaf extract based on the bleeding and lesions resulting from necrosis in kidney by using histopathology examination. Methods: The Stenochlaena palustris leaves were extracted using 95% ethanol and then given to male Wistar strain (Rattus norvegicus) with a 2,000, 2,500, and 3,000 mg/kg/body weight two times a day for fourteen days. The kidneys were collected and subjected to histopathology examination. Results: There are higher bleeding and necrosis lesion rates in the 2,500 and 3,000 mg/kg/body weight of Stenochlaena palustris leaves extract group compared to the control and 2,000 mg/kg/body weight of Stenochlaena palustris leaves extract group (p<0.05). Conclusion: Stenochlaena palustris leaf extract showed no toxic effect at doses of 2,000 mg/kg/body weight.","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49219367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"What the graduate in dentistry should be capable of doing in the hospital setting--the aims of the hospital dental program.","authors":"K M Gordon","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"45 4","pages":"177"},"PeriodicalIF":0.0,"publicationDate":"1979-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11499272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The teaching of anaesthesia to dental students in hospital.","authors":"G Albert","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"45 4","pages":"172"},"PeriodicalIF":0.0,"publicationDate":"1979-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11499268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Operating room protocol in the undergraduate dental curriculum.","authors":"A G Parnell","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75790,"journal":{"name":"Dental journal","volume":"45 4","pages":"178"},"PeriodicalIF":0.0,"publicationDate":"1979-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11499134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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