A patient with residual suprasellar cystic neoplasm was treated by direct instillation of the 32P colloid. The incidental bremsstrahlung images on a conventional gamma camera helped to localize the pharmaceutical within and outside of the lesion. Advantages of bremsstrahlung imaging and the dosimetry results of phantom studies carried out are described.
The localization of 111In activity in the tumor and draining lymph nodes of the H-4-II-E ACI rat hepatoma was investigated following the injection of 111In-chloride. In this tumor model, the tumors metastasize to the regional lymph nodes in male rats only. The following experiments were performed: (a) biodistribution of 111In; (b) correlation of 111In uptake with [3H]thymidine; (c) γ camera imaging; (d) autoradiography; (e) iron competition and (f) binding of 131I-transferrin to H-4-II-E cells. Tumor-to-muscle ratios of 111In in males were 4.9:1 in the primary tumor and 9.1:1 in the metastatic lymph nodes 24h post injection. In the lymph node metastases in the males, a significant correlation between 111In uptake and [3H]thymidine was observed (r = 0.737) suggesting that 111In uptake in the metastases is related to cellular proliferation. No such correlation was observed in either primary tumors (both male and female) or in the draining lymph nodes of the females. Metastatic lymph nodes in males could be detected in γ camera images while draining nodes in females could not be delineated. Injection of ferric citrate prior to 111In administration resulted in a significant reduction of 111In uptake in the liver, spleen and tumor and increased the amount of activity recovered from the kidney. Measurements of the binding of 131I-labeled rat transferrin to H-4-II-E cells in vitro suggest that these cells display transferrin receptors.
Les études radioisotopiques hépatobiliaires utilisant le 99mTc-diethyl-IDA ont permis de reconsidérer sur de nouvelles bases ]'exploration morphologique et fonctionnelle des anastomoses bilio-digestives.
Notre étude clinique concerne 31 patients (13 choledocoduodenostomies 10 hepaticojéjunostomies, 6 choledocojéjunostomies et 2 cholecystojéjunostomies). Elle permet de préciser l'imagerie scintigraphique (stase dans les canaux biliaire intra-hépatiques, reflux dans l'estomac, phénomène de strangulation au niveau mésocolon, obstruction complete ou incomplete). Nous discutons des diflérents problèmes associes aux procedures diagnostiques courantes et situons la position de la méthode scintigraphique parmi les techniques radiologiques (transit baryte, cholangiographie endoscopique retrograde, cholangiographie transhépatique).
Cette procedure diagnostique non invasive a un intérêt de premier plan dans l'exploration des anastomoses bilio-digestives et en particulier dans les hepaticojéjunostomies,
We describe the conjugation of DTPA to 100-500 micrograms of protein in concentrations of 0.6-1.0 mg/mL utilizing the mixed anhydride method. Free DTPA is removed by minicolumn gel filtration and centrifugation with minimal protein dilution. Radiolabelling at any selected pH can be achieved easily by diluting the protein in the desired buffer. The radiolabelling process can be monitored by instant thin layer chromatography. Any radiochemical impurity detected can be eliminated either by additional minicolumn filtration or further chelation with more conjugated protein. In citrate buffer at pH 6 with minicolumn gel chromatography we prepared 111In-DTPA-D3 (3.0 microCi/micrograms) monoclonal antibody and used it to image hepatocarcinoma in guinea pigs.
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