International journal of nuclear medicine and biology最新文献

L’élévation de la température interne du lapin après injection intraveineuse d’une solution d’endotoxine est proportionnelle à la concentration. La coagulation d’un lysat d’amoebocytes de Limule lorsqu'il est placé en présence d’une solution d’endotoxine a aussi pour facteur la concentration. Nous avons comparé la sensibilité des deux réactions. Dans nos conditions expérimentales, le lapin se montre sensible à une solution contenant 0,40 ng/mL et le lysat d’amoebocytes de Limule, à une solution contenant 0,14 ng/mL. La sensibilité du lapin croît avec le volume injecté par kilogramme alors que pour le lysat cette sensibilité n'est pas liée directement au volume de solution à contrôler.

The rise of the rabbit internal temperature after i.v. injection of an endotoxin solution is proportional to concentration. Gelation of Limulus amoebocyte, when in presence of an endotoxin solution, is also related to concentration. We compared the sensitivity of these two methods. With our experimental procedure, the rabbit is sensitive to a 0.40 ng/mL solution and the Limulus amoebocyte lysate to a 0.14 ng/mL solution. The rabbit sensitivity increase is related to the per kilogramme injected volume, whereas sensitivity is not related to the volume to check in the case of the lysate.

The preparation of ^99mTc(Sn)MDP was investigated as a function of pH, MDP concentration and Sn(II) concentration. The labeling efficiency was over 90% in the majority of the experiments and remained constant over the range pH 2–9. The MDP concentration had little effect, while the Sn(II) concentration had a significant positive influence. The complex formation appeared to be partly reversible.

The formation of different complexes was investigated by means of gel chromatography under various experimental conditions. Altogether six complexes were found. At acid conditions two major complexes were found and at neutral pH one major complex. The presence or absence of a particular complex was mainly determined by the pH and by the MDP concentration. The Sn(II) concentration had very little effect.

The results are compared with previous results of similar experiments with ^99mTc(Sn)pyrophosphate.(1)

The lymph node uptake of ¹⁶⁵Dy was measured in 25 patients treated by radiation synovectomy via intra-articular injection of ^l65Dy-ferric hydroxide macroaggregates (FHMA). An average of 0.12% of the injected dose was found in the inguinal lymph nodes 19h post injection. This results in a lymph node of 16.6 rad (166mGy), a dose significantly less than that reported following radiation synovectomy with other radiocolloids. Dosimetry calculations for the intra-articular injection of ¹⁶⁵Dy-FHMA are provided in the appendix.

L’élévation de la température interne du lapin après injection d’endotoxine varie en fonction de la voie utilisée. Une élévation de température de 1,71 ± 0,411°C est obtenue après injection intraveineuse de 1 ng/kg d’endotoxine d’Escherichia coli 0111.B.4. L’injection intrathécale par la voie sous occipitale de 0,1 ng/kg entraîne une élévation de 1,93 ± 0,236°C; l’analyse statistique des résultats montre que la réaction hyperthermique du lapin est dix fois plus intense lorsque l’endotoxine est injectée par cette voie.

The rise of the rabbit internal temperature after endotoxin injection is related to the route of administration. A rise of 1.71 ±0.411°C is obtained after i.v. injection of 1 ng/kg Escherichia coli 0111.B.4 endotoxin. An increase of 1.93 ±0.236°C is obtained after suboccipital intrathecal injection of 0.1 ng/kg of the same endotoxin; with the intrathecal route, the hyperthermia is induced by E. coli endotoxin after a dose ten times lower than with i.v. injection as shown by statistical analysis.

The binding of plutonium to human apo-transferrin and to rat serum was investigated following delivery of the metal to the protein either as the plutonium-tri-n-butyl phosphate (Pu-TBP) complex in n-dodecane or as plutonium nitrate. Chromatographic behaviour, the failure to bind to iron-saturated transferrin and the release of plutonium by the chelating agents CaNa₃DTPA and 3,4,3-LICAM(C) suggest that the transferrin complexes formed from the two plutonium compounds are similar. The tetracatechoylamide ligand LICAM(C) was found to be about 500 times more effective than DTPA, on a molar basis, for the release of plutonium from transferrin in rat serum.

The adsorption of pyrophosphate, tin-pyrophosphate and 99mTc(Sn)pyrophosphate on Ca₃(PO₄)₂ was investigated at pH 4.0 and pH 7.4. All components were radioactively labeled.

Tin and reduced technetium were in most cases almost completely bound. The adsorption of pyrophosphate, tin(II) and technetium-99m at pH 4.0 was higher than at pH 7.4. The presence of tin gave rise to an increase of the pyrophosphate adsorption that was much larger than can be accounted for by a stoichiometric adsorption of tin-pyrophosphate.

It is concluded that tin and technetium are bound as negatively charged complexes with pyrophosphate.

Finally it is argued that the fraction of the bone scanning agent that reaches the bone surface is adsorbed completely by the mineral phase.