This quasi-experimental study aimed to investigate the impact of Damask rose (DR) aromatherapy on the sleep quality of mothers with preterm infants in neonatal intensive care units (NICUs). Seventy-five eligible mothers were non-randomly divided into intervention and control groups. The control group received routine care, while the intervention group received 10% DR aromatherapy 20 min before bedtime for 10 nights. Data collection included a demographic questionnaire and the Pittsburgh Sleep Quality Index (PSQI). Statistical analysis was conducted using Chi-square, Mann–Whitney, and Wilcoxon tests with a significance level of p < 0.05 (SPSS 16). Results revealed a significant improvement in sleep quality and its subscales in the intervention group (p < 0.05). Although both groups showed improved total sleep scores (p < 0.05), the intervention group exhibited a substantially greater improvement (8.31 ± 1.82 to 5.62 ± 1.91) compared to the control group (9.95 ± 2.89 to 9.47 ± 3.30), as indicated by the estimated effect size. This study demonstrates that DR aromatherapy can enhance the sleep quality of mothers with preterm infants in NICUs, suggesting its potential as a supportive intervention in neonatal care settings.
{"title":"The impact of aromatherapy with Damask rose on the sleep quality of mothers with premature newborns: a quasi-experimental study","authors":"Fatemeh Hashemi, Ghazaleh Heydarirad, Fatemeh Asadollah, Lida Nikfarid","doi":"10.1007/s13596-024-00744-3","DOIUrl":"10.1007/s13596-024-00744-3","url":null,"abstract":"<div><p>This quasi-experimental study aimed to investigate the impact of Damask rose (DR) aromatherapy on the sleep quality of mothers with preterm infants in neonatal intensive care units (NICUs). Seventy-five eligible mothers were non-randomly divided into intervention and control groups. The control group received routine care, while the intervention group received 10% DR aromatherapy 20 min before bedtime for 10 nights. Data collection included a demographic questionnaire and the Pittsburgh Sleep Quality Index (PSQI). Statistical analysis was conducted using Chi-square, Mann–Whitney, and Wilcoxon tests with a significance level of <i>p</i> < 0.05 (SPSS 16). Results revealed a significant improvement in sleep quality and its subscales in the intervention group (<i>p</i> < 0.05). Although both groups showed improved total sleep scores (<i>p</i> < 0.05), the intervention group exhibited a substantially greater improvement (8.31 ± 1.82 to 5.62 ± 1.91) compared to the control group (9.95 ± 2.89 to 9.47 ± 3.30), as indicated by the estimated effect size. This study demonstrates that DR aromatherapy can enhance the sleep quality of mothers with preterm infants in NICUs, suggesting its potential as a supportive intervention in neonatal care settings.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"871 - 879"},"PeriodicalIF":1.8,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-21DOI: 10.1007/s13596-024-00743-4
Rasha E. M. Ali, Khaled M. Zayed, Ahmed A. Hamed, Mohamed R. Habib, Hanaa A. M. Abu ElEinin, Mosad A. Ghareeb, Rasha M. Gad El-Karim
The world urgently needs to discover new drugs to meet the challenges that threaten human health. Marine molluscs are known as natural sources of various bioactive compounds. In the present study, to evaluate the biological activity of the marine snail Planaxis sulcatus, soft tissues of the snail were extracted using acetone (Me2CO), ethanol (EtOH) and ethyl acetate (EtOAc). All extracts were tested for their total antioxidant capacity, total phenolic and DPPH free radical scavenging activity. The extracts were also tested in vitro for their antimicrobial activities. The results showed that, EtOAc extract of P. sulcatus had the highest antioxidant capacity and total phenolic content, followed by acetone extract. Concerning antimicrobial and antibiofilm activities of tested extracts, EtOH extract showed a good antimicrobial and antibiofilm activity against Klebsiella pneumoniae, Staphylococcus aureus and Proteus vulgaris. The most promising extract, EtOH extract, was processed for cytotoxicity testing and GC–MS analysis based on antimicrobial testing. Ethanol extract revealed a significant cytotoxic effect against hepatocellular carcinoma (HePG-2) and colorectal colon cancer cell lines. GC–MS analysis indicated the presence of 41 compounds in the EtOH extract with hexadecanoic acid, ethyl ester and octadecanoic acid, ethyl ester as the main ingredients consisting 16.55% and 14.56% of the total content, respectively. These data suggest that P. sulcatus could be a good source for many compounds with antimicrobial and anticancer potentials.
{"title":"Bioactive potential and chemical characterization of Planaxis sulcatus (Phylum: Mollusca, Family: Planaxidae) solvent extracts","authors":"Rasha E. M. Ali, Khaled M. Zayed, Ahmed A. Hamed, Mohamed R. Habib, Hanaa A. M. Abu ElEinin, Mosad A. Ghareeb, Rasha M. Gad El-Karim","doi":"10.1007/s13596-024-00743-4","DOIUrl":"10.1007/s13596-024-00743-4","url":null,"abstract":"<div><p>The world urgently needs to discover new drugs to meet the challenges that threaten human health. Marine molluscs are known as natural sources of various bioactive compounds. In the present study, to evaluate the biological activity of the marine snail <i>Planaxis sulcatus</i>, soft tissues of the snail were extracted using acetone (Me<sub>2</sub>CO), ethanol (EtOH) and ethyl acetate (EtOAc)<i>. </i>All extracts were tested for their total antioxidant capacity, total phenolic and DPPH free radical scavenging activity. The extracts were also tested in vitro for their antimicrobial activities. The results showed that, EtOAc extract of <i>P. sulcatus</i> had the highest antioxidant capacity and total phenolic content, followed by acetone extract. Concerning antimicrobial and antibiofilm activities of tested extracts, EtOH extract showed a good antimicrobial and antibiofilm activity against <i>Klebsiella pneumoniae, Staphylococcus aureus and Proteus vulgaris</i>. The most promising extract, EtOH extract, was processed for cytotoxicity testing and GC–MS analysis based on antimicrobial testing. Ethanol extract revealed a significant cytotoxic effect against hepatocellular carcinoma (HePG-2) and colorectal colon cancer cell lines. GC–MS analysis indicated the presence of 41 compounds in the EtOH extract with hexadecanoic acid, ethyl ester and octadecanoic acid, ethyl ester as the main ingredients consisting 16.55% and 14.56% of the total content, respectively. These data suggest that <i>P. sulcatus</i> could be a good source for many compounds with antimicrobial and anticancer potentials.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"859 - 870"},"PeriodicalIF":1.8,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142412905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.1007/s13596-024-00742-5
Virginia Moura Oliveira, Letícia Rafaela Silveira, Kitete Tunda Bunnel, Caroline Pereira Domingueti, André Oliveira Baldoni, Nayara Ragi Baldoni, Renê Oliveira do Couto
We report on the systematic review and meta-analysis concerning the efficacy of R. officinalis in treating diabetes mellitus (DM) in animals. This study followed the PRISMA guideline and the protocol was registered in PROSPERO (CRD42021250556). The research was duplicated in the PubMed, Scopus, ScienceDirect, Web of Science, and Virtual Health Library (VHL) databases until December 31st, 2022. No restrictions have been set for language publication. Twenty-three (23) experimental studies of type-1 diabetes mellitus (T1DM) met the eligibility criteria and were included in the qualitative analysis, whereas eighteen (18) underwent a meta-analysis. The R. officinalis derivatives significantly decreased fasting plasma glucose (MD: −120.84 [95% CI; −157.09, −84.59]); increased insulin release (MD; +3.73 [95% CI; +3.17, +4.29]); dwindled blood urea nitrogen (MD: −24.84 [95% CI; −34.78, −14.90]) and creatinine (MD: −0.40 [95% CI; −0.74, −0.06]) levels; and ameliorated liver function or repaired liver damage by decreasing ALT (MD: −36.42; [95% CI; −55.69, −17.14]) and AST (MD: −24.05 [95% CI; −37.84, −10.27]) enzyme levels compared to vehicle control group. Moreover, R. officinalis derivatives improved the lipid profile of diabetic animals by reducing LDL-c levels (MD: −11.74 [95% CI; −21.27, −2.21]). R. officinalis is a nutraceutical that may help in the management of T1DM and its complications. However, some gaps need to be taken into account for this evidence. Greater attention is needed for an analytical standardization of Rosemary extracts besides the demand for high-quality clinical studies dealing with the efficacy of this phytomedicine.
Graphical abstract
{"title":"Rosemary (Rosmarinus officinalis L.) improves biochemical outcomes in diabetes mellitus: a systematic review and meta-analysis of animal studies","authors":"Virginia Moura Oliveira, Letícia Rafaela Silveira, Kitete Tunda Bunnel, Caroline Pereira Domingueti, André Oliveira Baldoni, Nayara Ragi Baldoni, Renê Oliveira do Couto","doi":"10.1007/s13596-024-00742-5","DOIUrl":"10.1007/s13596-024-00742-5","url":null,"abstract":"<div><p>We report on the systematic review and meta-analysis concerning the efficacy <i>of R. officinalis</i> in treating diabetes mellitus (DM) in animals. This study followed the PRISMA guideline and the protocol was registered in PROSPERO (CRD42021250556). The research was duplicated in the PubMed, Scopus, ScienceDirect, Web of Science, and Virtual Health Library (VHL) databases until December 31st, 2022. No restrictions have been set for language publication. Twenty-three (23) experimental studies of type-1 diabetes mellitus (T1DM) met the eligibility criteria and were included in the qualitative analysis, whereas eighteen (18) underwent a meta-analysis. The <i>R. officinalis</i> derivatives significantly decreased fasting plasma glucose (MD: −120.84 [95% CI; −157.09, −84.59]); increased insulin release (MD; +3.73 [95% CI; +3.17, +4.29]); dwindled blood urea nitrogen (MD: −24.84 [95% CI; −34.78, −14.90]) and creatinine (MD: −0.40 [95% CI; −0.74, −0.06]) levels; and ameliorated liver function or repaired liver damage by decreasing ALT (MD: −36.42; [95% CI; −55.69, −17.14]) and AST (MD: −24.05 [95% CI; −37.84, −10.27]) enzyme levels compared to vehicle control group. Moreover, <i>R. officinalis</i> derivatives improved the lipid profile of diabetic animals by reducing LDL-c levels (MD: −11.74 [95% CI; −21.27, −2.21]). <i>R. officinalis</i> is a nutraceutical that may help in the management of T1DM and its complications. However, some gaps need to be taken into account for this evidence. Greater attention is needed for an analytical standardization of Rosemary extracts besides the demand for high-quality clinical studies dealing with the efficacy of this phytomedicine.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"1 - 26"},"PeriodicalIF":1.8,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-09DOI: 10.1007/s13596-023-00741-y
Renzhi Hu, Min Xia, Shuangya Weng, Zhi Chen, Zhen Wang, Xuemei Zou, Yan Zhang, Yiming Chen, Sisi Tang
Kidney and blood refreshing recipe formula is usually used to effectively treat intrauterine adhesions (IUAs). However, little is known about the underlying mechanism of kidney and blood refreshing recipes. We investigated the pharmacological mechanism of Bushen Yixue recipe on IUA through pharmacological and experimental validation. By network pharmacological analysis, 183 active constituents of the kidney and blood refreshing recipe were screened out (retrieved from TCMSP and TCM-ID). According to Venn diagram analysis, there were 102 common targets of the kidney and blood refreshing recipe. PPI analysis of the above common targets showed that there were 102 nodes and 1678 edges. Subsequently, GO enrichment and KEGG pathway analyses were also performed, which resulted in 1582 biological processes, 111 molecular functions, 82 cell components, and 174 related pathways. The active component of the kidney and blood refreshing recipe was also determined by liquid mass spectrometry. Finally, a rat model of IUA was constructed and tested in vivo. After kidney and blood refreshing recipe treatment, HE staining, Masson staining and immunohistochemistry (TGF-β1, SDF-1, VEGF) were performed on the uterine tissues of rats. The results showed that fibrosis in the uterine tissue was inhibited. The kidney and blood refreshing recipe inhibits fibrosis by inhibiting the expression of genes typical of fibrosis.
补肾养血方通常用于有效治疗宫腔内粘连(IUA)。然而,人们对补肾活血方的内在机制知之甚少。我们通过药理和实验验证,研究了布神益血方治疗宫腔粘连的药理机制。通过网络药理分析,筛选出补肾养血方中的 183 种有效成分(检索自 TCMSP 和 TCM-ID)。根据维恩图分析,补肾养血方有 102 个共同靶点。对上述共同靶标的 PPI 分析表明,共有 102 个节点和 1678 条边。随后,还进行了 GO 富集和 KEGG 通路分析,得出了 1582 个生物过程、111 个分子功能、82 个细胞成分和 174 条相关通路。此外,还通过液质联用法测定了肾脏和血液清凉配方中的活性成分。最后,构建了 IUA 大鼠模型并进行了体内试验。经过补肾养血方治疗后,对大鼠子宫组织进行了 HE 染色、Masson 染色和免疫组化(TGF-β1、SDF-1、VEGF)。结果表明,子宫组织的纤维化得到了抑制。肾脏和血液清凉配方通过抑制纤维化典型基因的表达来抑制纤维化。
{"title":"Network pharmacology and experimental validation to explore the molecular mechanisms of kidney and blood refreshing recipe for the treatment of intrauterine adhesions","authors":"Renzhi Hu, Min Xia, Shuangya Weng, Zhi Chen, Zhen Wang, Xuemei Zou, Yan Zhang, Yiming Chen, Sisi Tang","doi":"10.1007/s13596-023-00741-y","DOIUrl":"10.1007/s13596-023-00741-y","url":null,"abstract":"<div><p>Kidney and blood refreshing recipe formula is usually used to effectively treat intrauterine adhesions (IUAs). However, little is known about the underlying mechanism of kidney and blood refreshing recipes. We investigated the pharmacological mechanism of Bushen Yixue recipe on IUA through pharmacological and experimental validation. By network pharmacological analysis, 183 active constituents of the kidney and blood refreshing recipe were screened out (retrieved from TCMSP and TCM-ID). According to Venn diagram analysis, there were 102 common targets of the kidney and blood refreshing recipe. PPI analysis of the above common targets showed that there were 102 nodes and 1678 edges. Subsequently, GO enrichment and KEGG pathway analyses were also performed, which resulted in 1582 biological processes, 111 molecular functions, 82 cell components, and 174 related pathways. The active component of the kidney and blood refreshing recipe was also determined by liquid mass spectrometry. Finally, a rat model of IUA was constructed and tested in vivo. After kidney and blood refreshing recipe treatment, HE staining, Masson staining and immunohistochemistry (TGF-β1, SDF-1, VEGF) were performed on the uterine tissues of rats. The results showed that fibrosis in the uterine tissue was inhibited. The kidney and blood refreshing recipe inhibits fibrosis by inhibiting the expression of genes typical of fibrosis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"845 - 857"},"PeriodicalIF":1.8,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-023-00741-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139850655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-05DOI: 10.1007/s13596-023-00740-z
Khushbu Nishad, Talambedu Usha, H. P. Prashanth Kumar, Nijalingappa Ramesh, Sushil Kumar Middha
A conventional plant flavonoid known as myricetin is well-known for its wide range of effects, including its strong anti-inflammatory, anti-cancer, and antioxidant characteristics. In this study, we examined anti-inflammatory potential of Myricetin (MN) by looking at the expressions of COX-2, IL-6 and TNF-α in leukemia cancer cell lines (THP-1). The THP-1 was cultured in Roswell Park Memorial Institute medium supplemented with 10% foetal bovine serum and penicillin (100 U/ml) for the purposes of the in-vitro investigations. Cell viability and cytotoxicity testing were done using the MTT assay. To prepare the ligand and proteins for the in-silico studies, The structures of the targeted anti-inflammatory proteins COX-2 (PDB ID: 5KIR), TNF- (PDB ID: 1TNF), and IL-6 (PDB ID: 1ALU) were retrieved from Protein Data Bank. Furthermore, docking experiments were carried out using the AutoDock Vina software. In comparison to the reference drug cisplatin (9.46 µg/ml) the IC50 value for MN was found to be 90.161 µg/ml. The cells pretreated with MN showed a significantly lower expression of anti-inflammatory components (COX-2, TNF-α and IL6) upon measured by geometric mean fluorescence intensity as compared to the LPS-induced control cells. Additionally, MN has shown a strong interaction with COX-2, TNF-α and IL-6 in simulation studies. To conclude, MN exhibits a potent anti-inflammatory effect both in-silico and in-vitro studies. Further, in vivo investigations are needed to gain more comprehensive understanding of the molecule.
{"title":"Anti-inflammatory potential of myricetin in leukemia cells: in silico and in vitro exploration","authors":"Khushbu Nishad, Talambedu Usha, H. P. Prashanth Kumar, Nijalingappa Ramesh, Sushil Kumar Middha","doi":"10.1007/s13596-023-00740-z","DOIUrl":"10.1007/s13596-023-00740-z","url":null,"abstract":"<div><p>A conventional plant flavonoid known as myricetin is well-known for its wide range of effects, including its strong anti-inflammatory, anti-cancer, and antioxidant characteristics. In this study, we examined anti-inflammatory potential of Myricetin (MN) by looking at the expressions of COX-2, IL-6 and TNF-α in leukemia cancer cell lines (THP-1). The THP-1 was cultured in Roswell Park Memorial Institute medium supplemented with 10% foetal bovine serum and penicillin (100 U/ml) for the purposes of the in-vitro investigations. Cell viability and cytotoxicity testing were done using the MTT assay. To prepare the ligand and proteins for the in-silico studies, The structures of the targeted anti-inflammatory proteins COX-2 (PDB ID: 5KIR), TNF- (PDB ID: 1TNF), and IL-6 (PDB ID: 1ALU) were retrieved from Protein Data Bank. Furthermore, docking experiments were carried out using the AutoDock Vina software. In comparison to the reference drug cisplatin (9.46 µg/ml) the IC50 value for MN was found to be 90.161 µg/ml. The cells pretreated with MN showed a significantly lower expression of anti-inflammatory components (COX-2, TNF-α and IL6) upon measured by geometric mean fluorescence intensity as compared to the LPS-induced control cells. Additionally, MN has shown a strong interaction with COX-2, TNF-α and IL-6 in simulation studies. To conclude, MN exhibits a potent anti-inflammatory effect both in-silico and in-vitro studies. Further, in vivo investigations are needed to gain more comprehensive understanding of the molecule.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"835 - 844"},"PeriodicalIF":1.8,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139862978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-27DOI: 10.1007/s13596-023-00738-7
Sylvester I. Omoruyi, Joshua Delport, Tusekile S. Kangwa, Ziyaad Rahman, Ahmed A. Hussein, Dietrich E. Lorke, Okobi E. Ekpo
Polyphenols are notable phytochemicals mostly found in plant-based foods, with many reported health benefits. Only limited information is available in the literature on polyphenolic lignans; hence, this review highlights the findings from an extensive open-ended search performed on many databases and search engines, using the keywords “phyllanthin” and “hypophyllanthin” inserted separately and jointly into these databases. Up to 2700 distinct articles were generated, most of which were related to plants of the Phyllanthus species. Plant extract-based articles were excluded. 468 original articles and bibliographic reviews that met the inclusion criteria were selected. Our search showed that both lignans were mostly isolated from plant sources, and some of their biological and pharmacological activities have been previously studied in in silico, in vitro and in vivo experimental models. Only a few human studies were reported, mainly in ex-vivo studies with blood samples and parasites. The antioxidant, anti-inflammatory and immunomodulatory, cardiovascular and cardioprotective, antimicrobial, antiviral, antiparasitic, neuroprotective and neuroactive, anti-diabetic, reno-protective and uricosuric, hepatoprotective, metabolic as well as anticancer effects are well-documented and described in the different sections of this review, as summarized in Table 1. No direct toxicity and side effects were reported. The health benefits identified in the reviewed articles potentially provide some rationale for further scientific research on these lignans’ toxicology, safety profiles, pharmacokinetics, and pharmacodynamics, with a view to progressing through the clinical trial phases of the drug discovery and development process.
{"title":"An update on the bioactivities and health benefits of two plant-derived lignans, phyllanthin and hypophyllanthin","authors":"Sylvester I. Omoruyi, Joshua Delport, Tusekile S. Kangwa, Ziyaad Rahman, Ahmed A. Hussein, Dietrich E. Lorke, Okobi E. Ekpo","doi":"10.1007/s13596-023-00738-7","DOIUrl":"10.1007/s13596-023-00738-7","url":null,"abstract":"<div><p>Polyphenols are notable phytochemicals mostly found in plant-based foods, with many reported health benefits. Only limited information is available in the literature on polyphenolic lignans; hence, this review highlights the findings from an extensive open-ended search performed on many databases and search engines, using the keywords “phyllanthin” and “hypophyllanthin” inserted separately and jointly into these databases. Up to 2700 distinct articles were generated, most of which were related to plants of the <i>Phyllanthus</i> species. Plant extract-based articles were excluded. 468 original articles and bibliographic reviews that met the inclusion criteria were selected. Our search showed that both lignans were mostly isolated from plant sources, and some of their biological and pharmacological activities have been previously studied in in silico, in vitro and in vivo experimental models. Only a few human studies were reported, mainly in ex-vivo studies with blood samples and parasites. The antioxidant, anti-inflammatory and immunomodulatory, cardiovascular and cardioprotective, antimicrobial, antiviral, antiparasitic, neuroprotective and neuroactive, anti-diabetic, reno-protective and uricosuric, hepatoprotective, metabolic as well as anticancer effects are well-documented and described in the different sections of this review, as summarized in Table 1. No direct toxicity and side effects were reported. The health benefits identified in the reviewed articles potentially provide some rationale for further scientific research on these lignans’ toxicology, safety profiles, pharmacokinetics, and pharmacodynamics, with a view to progressing through the clinical trial phases of the drug discovery and development process.\u0000</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 4","pages":"1011 - 1036"},"PeriodicalIF":1.8,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139592366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The notoriety of toxic metals has become a cause for concern across the world as their presence in the environment constitutes serious health hazard that affect not only the human populace but the ecosystem. This study aimed to explore the ameliorative potential of ethanol extract of Stachytarpheta cayennensis (SCE) leaf in Wistar rats exposed to arsenic, cadmium, and lead (ACL). Thirty Wistar rats were divided into five (5) groups of six (6) rats each. Group one served as the control group was given 300 ml of distilled water as their daily drinking water. Group two as ACL group was exposed to a combination of 30 mg/kg of lead, cadmium, and 1 mg/kg of arsenic, daily, via drinking water. Group three, four and five were the treatment groups administered a combination of 100 mg/kg of vitamins C and 30 mg/kg of vitamin E, 200, and 400 mg/kg of SCE with ACL in daily drinking water respectively. Experiment lasted for 28 days, the rats were sacrificed, blood and other organs were harvested for laboratory analysis. There were varying degrees of significant (P<0.05) amelioration of the heavy metals toxicity revealed by positive changes in the biochemical and hematological indices evaluated when the treated groups were compared to the ACL group. Therefore, the administration of SCE, vitamin C and E could potentially protect against the co-induced toxicity of arsenic, cadmium and lead in Wistar rats.
有毒金属在环境中的存在严重危害人们的健康,不仅影响人类,还影响生态系统,因此,有毒金属的问题已引起全世界的关注。本研究旨在探索 Stachytarpheta cayennensis(SCE)叶乙醇提取物对暴露于砷、镉和铅(ACL)的 Wistar 大鼠的改善潜力。30 只 Wistar 大鼠被分为五(5)组,每组六(6)只。第一组为对照组,每天饮用 300 毫升蒸馏水。第二组作为 ACL 组,每天通过饮用水接触 30 毫克/千克的铅、镉和 1 毫克/千克的砷。第三组、第四组和第五组分别是维生素 C 100 毫克/千克、维生素 E 30 毫克/千克、SCE 200 毫克/千克和 ACL 400 毫克/千克组合的处理组。实验持续了 28 天,大鼠被处死,采集血液和其他器官进行实验室分析。与 ACL 组相比,治疗组的生化指标和血液学指标均发生了积极变化,表明重金属毒性有不同程度的明显改善(P<0.05)。因此,服用 SCE、维生素 C 和 E 有可能保护 Wistar 大鼠免受砷、镉和铅的共同毒性。
{"title":"Ameliorative effect of Stachytarpheta cayennensis extract and vitamins C and E on arsenic, cadmium and lead co-induced toxicity in Wistar rats","authors":"Osazuwa Clinton Ekhator, Elijah Eshiokwemia Igbokah, Marvin Wisdom Eromosele, Sherifat Onosioriamhe Harun, Blessing Ejiro Oghenegweke, Jessica Uchechukwu Egbe, Success Isuman","doi":"10.1007/s13596-023-00736-9","DOIUrl":"10.1007/s13596-023-00736-9","url":null,"abstract":"<div><p>The notoriety of toxic metals has become a cause for concern across the world as their presence in the environment constitutes serious health hazard that affect not only the human populace but the ecosystem. This study aimed to explore the ameliorative potential of ethanol extract of <i>Stachytarpheta cayennensis</i> (SCE) leaf in Wistar rats exposed to arsenic, cadmium, and lead (ACL). Thirty Wistar rats were divided into five (5) groups of six (6) rats each. Group one served as the control group was given 300 ml of distilled water as their daily drinking water. Group two as ACL group was exposed to a combination of 30 mg/kg of lead, cadmium, and 1 mg/kg of arsenic, daily, via drinking water. Group three, four and five were the treatment groups administered a combination of 100 mg/kg of vitamins C and 30 mg/kg of vitamin E, 200, and 400 mg/kg of SCE with ACL in daily drinking water respectively. Experiment lasted for 28 days, the rats were sacrificed, blood and other organs were harvested for laboratory analysis. There were varying degrees of significant (P<0.05) amelioration of the heavy metals toxicity revealed by positive changes in the biochemical and hematological indices evaluated when the treated groups were compared to the ACL group. Therefore, the administration of SCE, vitamin C and E could potentially protect against the co-induced toxicity of arsenic, cadmium and lead in Wistar rats.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"823 - 833"},"PeriodicalIF":1.8,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139601878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Except for South Africa, Africa recorded very low cases of coronavirus disease-19 (COVID-19), and even lower deaths compared to other regions of the world. Several reasons were proposed to explain these low numbers. In this review, we appraise severe acute respiratory syndrome-2 (SARS-CoV-2) therapeutic targets and varying empirical evidence on the use of anti-COVID-19 herbal and bioactive agents in Africa and the role of geo-demographic factors associated with low morbidity and mortality in Africa. Many of the traditional medicinal plants Africans use in the fight against COVID-19 are thought to possess some intrinsic antiviral properties against SARS-CoV-2. Some others were used to treat COVID-19-related symptoms with positive testimonies, making it necessary for in-depth research of African traditional medicine. The adequate knowledge of medicinal plants, their activities, dosage and appropriate duration of use will further advance traditional medical care and help researchers to identify ideal promising drug candidates for accelerated drug development. COVID-19 in sub-Saharan Africa is extremely complex and involves numerous unknown variables as well as region-specific factors that have often been overlooked or underestimated. Traditional, complementary, and alternative medicine in the form of plant extracts and phytochemicals have significant inhibitory effects against SARS-CoV-2 as demonstrated by both in silico and in vitro models and thus could slow replication and transmission of the virus.
{"title":"Dissecting the low morbidity and mortality during the COVID-19 pandemic in Africa: a critical review of the facts and fallacies","authors":"Shuaibu Abdullahi Hudu, Abdulgafar Olayiwola Jimoh, Kasimu Ghandi Ibrahim, Yahaya Mohammed, Kehinde Ahmad Adeshina, Ahmed Kolawole Jimoh, Jibril Abubakar, Dawoud Usman, Abdalla Mohamad Jama","doi":"10.1007/s13596-023-00739-6","DOIUrl":"10.1007/s13596-023-00739-6","url":null,"abstract":"<div><p>Except for South Africa, Africa recorded very low cases of coronavirus disease-19 (COVID-19), and even lower deaths compared to other regions of the world. Several reasons were proposed to explain these low numbers. In this review, we appraise severe acute respiratory syndrome-2 (SARS-CoV-2) therapeutic targets and varying empirical evidence on the use of anti-COVID-19 herbal and bioactive agents in Africa and the role of geo-demographic factors associated with low morbidity and mortality in Africa. Many of the traditional medicinal plants Africans use in the fight against COVID-19 are thought to possess some intrinsic antiviral properties against SARS-CoV-2. Some others were used to treat COVID-19-related symptoms with positive testimonies, making it necessary for in-depth research of African traditional medicine. The adequate knowledge of medicinal plants, their activities, dosage and appropriate duration of use will further advance traditional medical care and help researchers to identify ideal promising drug candidates for accelerated drug development. COVID-19 in sub-Saharan Africa is extremely complex and involves numerous unknown variables as well as region-specific factors that have often been overlooked or underestimated. Traditional, complementary, and alternative medicine in the form of plant extracts and phytochemicals have significant inhibitory effects against SARS-CoV-2 as demonstrated by both in silico and in vitro models and thus could slow replication and transmission of the virus.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 4","pages":"999 - 1010"},"PeriodicalIF":1.8,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139523205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-17DOI: 10.1007/s13596-023-00737-8
Anak Agung Ayu Putri Laksmidewi, I. Nyoman Bayu Mahendra, Andreas Soejitno, Aurelia Vania
Chemotherapy-induced cognitive impairment (CICI) is an impairment of memory, learning power, concentration, reasoning, executive function, attention, and visuospatial during and after chemotherapy exposures. No proven safe and effective therapeutic regimen are available to improve cognitive function in CICI patients. To evaluate the safety and effectiveness of curcumin extract to improve cognitive function in CICI patients. This study was a double-blind randomized controlled trial clinical trial in patients with cervical carcinoma who underwent a carboplatin-paclitaxel chemotherapy regimen from March to October 2021 at single center hospital. Subjects divided into two groups that received curcumin and placebo caplets by dose escalation method from 240 to 400 mg intermittently (14 days on and 7 days off) between chemotherapy cycles. Cognitive function was evaluated pre- and post-therapy using the AFI questionnaire, Stroop test, and MoCA-Ina. A total of 78 subjects were equally divided into the treatment and control groups. The percentage of drop-out, mortality, and adverse drug response were relatively comparable between each treatment arm. The group of subjects receiving curcumin extract experienced clinically and statistically significant improvements in cognitive function based on the Stroop test (∆ median 8.57 vs. 2.46; Z − 4.503 vs. − 1.762; p < 0.0001 vs. 0.078) and MoCA- Ina (∆ mean 1.53 vs. 0.72; Z − 2.99 vs. − 2.05; p < 0.003 vs. 0.04) versus placebo in between-group and between-subject analyses, respectively. Administration of curcumin extract with intermittent dose escalation regimen proved to be safe and able to improve cognitive function of CICI patients clinically and statistically significant.
化疗引起的认知功能障碍(CICI)是指化疗期间和化疗后记忆力、学习能力、注意力、推理能力、执行功能、注意力和视觉空间感受损。目前尚无经证实安全有效的治疗方案来改善 CICI 患者的认知功能。本研究旨在评估姜黄素提取物改善 CICI 患者认知功能的安全性和有效性。本研究是一项双盲随机对照临床试验,对象是2021年3月至10月在单中心医院接受卡铂-紫杉醇化疗方案的宫颈癌患者。受试者分为两组,在化疗周期之间,通过剂量递增法间歇服用姜黄素和安慰剂胶囊,剂量从 240 毫克到 400 毫克不等(服用 14 天,停药 7 天)。在治疗前后使用 AFI 问卷、Stroop 测试和 MoCA-Ina 评估认知功能。共有 78 名受试者被平均分为治疗组和对照组。各治疗组的辍学率、死亡率和药物不良反应相对相当。根据 Stroop 测试结果,接受姜黄素提取物治疗的一组受试者的认知功能在临床和统计学上都有显著改善(∆ 中位数 8.57 vs. 2.46;Z - 4.503 vs. 1.762;p &l)。- 在组间分析和受试者间分析中,姜黄素提取物与安慰剂相比(∆ 中位数 1.53 vs. 0.72; Z - 2.99 vs. - 2.05; p < 0.003 vs. 0.04),认知功能分别有明显改善(p < 0.0001 vs. 0.078)和改善(∆ 中位数 1.53 vs. 0.72; Z - 2.99 vs. - 2.05; p < 0.003 vs. 0.04)。采用间歇性剂量递增方案服用姜黄素提取物被证明是安全的,并能在临床和统计学上显著改善CICI患者的认知功能。
{"title":"Improving cognitive function with intermittent dose escalation of curcumin extract in chemotherapy-induced cognitive impairment patients: a randomized controlled trial","authors":"Anak Agung Ayu Putri Laksmidewi, I. Nyoman Bayu Mahendra, Andreas Soejitno, Aurelia Vania","doi":"10.1007/s13596-023-00737-8","DOIUrl":"10.1007/s13596-023-00737-8","url":null,"abstract":"<div><p>Chemotherapy-induced cognitive impairment (CICI) is an impairment of memory, learning power, concentration, reasoning, executive function, attention, and visuospatial during and after chemotherapy exposures. No proven safe and effective therapeutic regimen are available to improve cognitive function in CICI patients. To evaluate the safety and effectiveness of curcumin extract to improve cognitive function in CICI patients. This study was a double-blind randomized controlled trial clinical trial in patients with cervical carcinoma who underwent a carboplatin-paclitaxel chemotherapy regimen from March to October 2021 at single center hospital. Subjects divided into two groups that received curcumin and placebo caplets by dose escalation method from 240 to 400 mg intermittently (14 days on and 7 days off) between chemotherapy cycles. Cognitive function was evaluated pre- and post-therapy using the AFI questionnaire, Stroop test, and MoCA-Ina. A total of 78 subjects were equally divided into the treatment and control groups. The percentage of drop-out, mortality, and adverse drug response were relatively comparable between each treatment arm. The group of subjects receiving curcumin extract experienced clinically and statistically significant improvements in cognitive function based on the Stroop test (∆ median 8.57 vs. 2.46; Z − 4.503 vs. − 1.762;<i> p</i> < 0.0001 vs. 0.078) and MoCA- Ina (∆ mean 1.53 vs. 0.72; Z − 2.99 vs. − 2.05; <i>p</i> < 0.003 vs. 0.04) versus placebo in between-group and between-subject analyses, respectively. Administration of curcumin extract with intermittent dose escalation regimen proved to be safe and able to improve cognitive function of CICI patients clinically and statistically significant.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"813 - 822"},"PeriodicalIF":1.8,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-023-00737-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139617083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.1007/s13596-023-00735-w
Yesmi P. Ahumada-Santos, Gabriela López-Angulo, Rebeca M. Pinto-González, Aldo F. Clemente-Soto, José A. López-Valenzuela, Francisco Delgado-Vargas
Melanins are widely distributed biopolymers that exhibit important biological activities. However, fruit melanins have been scarcely studied. In this work, the antibiofilm, cellular antioxidant, anti-inflammatory, immunomodulatory, cytotoxic, and antimutagenic activities of soluble melanins (SMs) isolated from the Randia echinocarpa fruit (papache) were evaluated. The SMs inhibited biofilm formation in Staphylococcus aureus MDR and ATCC 43300 up to 60% at 1000 µg/mL; they presented a cellular antioxidant activity (60.02%) at 50 µg/mL, were immunomodulatory by increasing the peripheral blood mononuclear cells (PBMC) proliferation index (1.09 at 50 μg/mL), and inhibited HeLa cell proliferation by 77.39% (IC50 = 9.34 µg/mL). SMs were neither toxic nor mutagenic in the Salmonella Typhimurium YG1024 strain and inhibited the 1-nitropyrene mutagenicity by 30.2%. The biological activities of papache SMs support their potential to be used in nutraceutical and pharmaceutical formulations.
{"title":"Antibiofilm, cellular antioxidant, anti-inflammatory, immunomodulatory, cytotoxic, and antimutagenic activities of soluble melanins from Randia echinocarpa fruit","authors":"Yesmi P. Ahumada-Santos, Gabriela López-Angulo, Rebeca M. Pinto-González, Aldo F. Clemente-Soto, José A. López-Valenzuela, Francisco Delgado-Vargas","doi":"10.1007/s13596-023-00735-w","DOIUrl":"10.1007/s13596-023-00735-w","url":null,"abstract":"<div><p>Melanins are widely distributed biopolymers that exhibit important biological activities. However, fruit melanins have been scarcely studied. In this work, the antibiofilm, cellular antioxidant, anti-inflammatory, immunomodulatory, cytotoxic, and antimutagenic activities of soluble melanins (SMs) isolated from the <i>Randia echinocarpa</i> fruit (papache) were evaluated. The SMs inhibited biofilm formation in <i>Staphylococcus aureus</i> MDR and ATCC 43300 up to 60% at 1000 µg/mL; they presented a cellular antioxidant activity (60.02%) at 50 µg/mL, were immunomodulatory by increasing the peripheral blood mononuclear cells (PBMC) proliferation index (1.09 at 50 μg/mL), and inhibited HeLa cell proliferation by 77.39% (IC<sub>50</sub> = 9.34 µg/mL). SMs were neither toxic nor mutagenic in the <i>Salmonella</i> Typhimurium YG1024 strain and inhibited the 1-nitropyrene mutagenicity by 30.2%. The biological activities of papache SMs support their potential to be used in nutraceutical and pharmaceutical formulations.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"801 - 812"},"PeriodicalIF":1.8,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}