Advances in Traditional Medicine最新文献

Tetrapleura tetraptera Taub. (Fabaceae), commonly known as Aidan is ethnopharmacologically used for the management of health conditions such as diabetes mellitus, leprosy, epilepsy, and stroke in Nigeria. This study evaluated the anti-oxidoinflammatory properties of T. tetraptera methanol leaf extract (TTE) in lithium-pilocarpine-induced status epilepticus in Wistar rats. The extract was phytochemically screened and HPLC fingerprinting was performed. Animals were intraperitoneally administered with 127 mg/kg lithium chloride followed by 25 mg/kg pilocarpine 20 h later to induce status epilepticus. The animals were post treated with 50, 100 and 200 mg/kg TTE with 10 mg/kg valproic acid as the reference standard drug. Phytochemical screening of TTE confirmed the presence of tannins, phenols, alkaloids, terpenoids, flavonoids, saponins, glycosides and steroids. Quantitative phytochemical and antioxidant analyses of the extract indicated significant in vitro antioxidant and radical scavenging activity. HPLC analysis of the extract confirmed the presence of aridanin and polyphenols. TTE ameliorated redox imbalance by increasing markers for oxidative stress such as ferric reducing antioxidant power, glutathione level, catalase, glutathione S-transferase and superoxide dismutase activities. Moreover, TTE ameliorated pro-inflammatory events by reducing the level of the pro-inflammatory mediator nitric oxide, attenuating lipid peroxidation (which produces inflammatory lipid peroxidation-derived aldehydes), and decreasing the activities of xanthine oxidase and lactate dehydrogenase in the brain. These results indicated that the leaf of Tetrapleura tetraptera has therapeutic potential against status epilepticus by reversing oxidoinflammatory events. Tetrapleura tetraptera leaf extracts could be used to produce novel plant-based pharmaceuticals for treating status epilepticus and associated disorders.

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The study investigates the role of Anogeissus leiocarpus methanol stem bark extract (ALSE) on seizure, oxidative stress and cognitive performance in pentylenetetrazole (PTZ)-induced epilepsy in rat model. Thirty Wistar rats were allocated into five groups (n = 6). Groups 1 and 2 received normal saline intra-peritoneal (i.p) every day and PTZ (i.p) at 35 mg/kg every other day respectively. Groups 3–5 were given ALSE orally at (250 mg/kg and 500 mg/kg) and Diazepam at 4 mg/kg (i.p) respectively. Groups 3–5 were given PTZ (i.p) at 35 mg/kg every other day for 30 days, 30 min after ALSE and Diazepam administration. The rats were observed for seizure activities and also evaluated for cognitive functions. The rats were euthanized thereafter and the brain histology and oxidative stress biomarkers were evaluated. PTZ induction resulted into increased seizure activities leading to the development of kindling, oxidative stress, cognitive impairment and histological aberration of the hippocampus. However, pretreatment with ALSE decreased seizure activities, reversed oxidative stress and cognitive impairment and preserved hippocampal histology relative to the PTZ alone treated rats. Conclusively, ALSE was found to increase seizure latency, prevented cognitive decline, and decreased seizure activities induced by PTZ-kindling in rats. Additionally, ALSE ameliorates PTZ-induced oxidative stress and histological aberrations of the hippocampus. Hence, this study proposed that ALSE might be a promising tool for ameliorating seizure in epilepsy.

Psoralea corylifolia L. has been used in traditional Chinese and Ayurvedic medicine systems for management of various diseases. The various phytochemical constituents work in orchestric manner to treat diverse illnesses. Current pharmaco-therapies shown beneficiary role in treatment of dyslipidaemia but facing life threatening side effects. The usage of herbs increased worldwide and paves the way for development of pharmaceuticals for hyperlipidemia treatment. The main objective of present work was to investigate anti-hyperlipidemic activity and in-silico pancreatic lipase inhibitory potential of Psoralea corylifolia L. (PC) leaf extract. The existence of several phytoconstituents was confirmed by the chromatographic research and mainly includes the flavonoids and furanocoumarins. All studied phytoconstituents were found to have superior binding affinity than standard orlistat (− 7.1 kcal/mol), with docking score ranges from − 10.6 to − 7.3 kcal/mol. At 200 mg/kg/day the ethanolic leaf extract demonstrated highest lipid lowering action. Ethanolic leaf extract of Psoralea corylifolia revealed evidential antihyperlipidemic potential in a concentration dependent manner (P < 0.01). The serum lipid profile (LDL, VLDL, TG, TC) dropped firmly and HDL elevated in hyperlipidemic rats treated with plant extract compared with the hyperlipidemic group rats (P < 0.01). The hepatic TC and TG abruptly increased in hyperlipidemic rats and significantly reduced in hyperlipidemic rats administered with EPC compared with the control group (P < 0.01). The hyperlipidemic rats treated with atorvastatin and PC at different doses shown evidentiary increase in secretion of TC and TG compared with the hyperlipidemic group rats. The study results proposed that EPC leaf extract demonstrated noteworthy antihyperlipidemic action. The findings of docking study recommend utilization of the best ligands experimentally to develop novel anti-obesity agents.

Traditional herbal medicine has been playing an essential role in primary health care globally. The aim of this work is to present an overview of traditional herbal medicine research productivity over the past years. The data was accessed from the Scopus database (www.scopus.com), while VOSviewer.Var1.6.6, Bibliometrix, and R studio were used for further analysis of the obtained data. The results showed that researches on traditional herbal medicine increased annually after 1990, followed by a corresponding increase in global citations during the period, with a total of 22,071 authors contributing to all the publications. Yiling Wang of Shanghai Institute of Drug Control, Shanghai, China was the most productive author (TNP = 303), while Journal of “Evidence-based Complementary and Alternative Medicine”, and “Journal of Ethnopharmacology” were the top ranked journals, respectively. Also, China, Japan, and India were found to be the top Corresponding Author's Countries for researches on traditional herbal medicine, as Beijing University of Chinese Medicine, China Academy of Chinese Medical Sciences and China Medical University were top affiliations. Moreover, National Natural Science Foundation of China, National Key Research and Development Program of China, Ministry of Science and Technology of the People's Republic of China, and Ministry of Science and Technology, Taiwan were top funding agencies, with more than 100 documents. The bibliometric research study has revealed an annual increasing trend in traditional herbal medicine, while also revealing that the topmost ranked authors and funding agencies were from Asia especially China.

Emergence of antiviral drug resistance in influenza virus remains a major public health concern worldwide. Nowadays, different herbs receive renewed attention because of their enormous antiviral potential. In this study, we investigated the antiviral activity of Camellia sinensis, Persicaria hydropiper, Persicaria orientale, Persicaria lapathifolia, Persicaria stagnina, Mucuna pruriens and Chenopodium album against different influenza strains using both in vitro and in silico approaches. Antiviral effect of plant extracts was evaluated by cytopathic effect (CPE) inhibition assay on influenza infected MDCK (Madin Darby Canine Kidney) cell line. Later, the herb demonstrating antiviral activity was virtually screened for their available bioactive compounds and multiple in silico tools were performed to prioritize and establish these compounds as potential inhibitor. The methanol, but not the n-hexane and ethyl acetate extracts of C. sinensis, P. hydropiper, M. pruriens and C. album exhibited anti-influenza effect with EC₅₀ values within 32–46 µg/ml. Importantly, the extracts remained effective against both amantadine-resistant and -sensitive influenza isolates. The molecular docking analysis showed that flavonoids, steroid and derivatives had strong binding affinity to the target proteins which may remain responsible for the anti-influenza characteristics of plant extracts. Pharmacokinetic properties, bioavailability and drug-likeness score revealed that ferulic acid, sinapic acid, campesterol, cryptomeridiol, eupatin and genistein could be attractive leads as potential influenza inhibitors. Taken together, the botanical ingredients of these herbs could be used as valuable candidates for developing novel therapeutics to control influenza related illnesses.

Nanoscale drug carriers are useful in improving the bioavilability, targeting delivery, and controlling the release of the loaded drug. Polymers from natural sources possess favourable properties such as adaptability and safety for usage as nanosized drug delivery carriers and as substitutes of synthetic polymers. The use of a biomaterial imparts special biopharmaceutical characteristics to the formulation and changes the pharmacokinetic and pharmacodynamic profile of the entrapped medication. Proteins appear as promising raw materials in this approach because of their extensive availability from renewable sources, low cost, and ability to be chemically modified, ligand conjugation and degraded into harmless by-products. Furthermore, protein nanocarriers have several benefits, including high drug-binding capability and specific tumour targeting using different ligands. This review discusses the properties of different protein biopolymers such as albumin, gelatin, zein, gliadin, casein, collagen, elastin and whey protein. The study focuses on the most relevant applications of the protein nanoparticles loading agents with antitumeric effect. Furthermore, the review summarises the primary findings of tumour-targeted protein nanoparticles in vitro and in vivo studies.

An ethnobotanical survey was conducted in Katsina state, Northern Nigeria to document medicinal plants commonly used by lactating mothers to boost breast milk production. Two hundred and forty (240) respondents made up of (120) nursing mothers, (60) herbalists and (60) traditional birth attendants were interviewed. Ethnobotanical data was obtained using the structured questionnaire method. Standard procedures were followed to analyse the phyto-chemical, proximate, mineral concentrations and chemical composition of the ethanolic extract of the most cited plants. Forty (40) plant species belonging to 38 genera distributed among 24 families are used as galactagogues in Katsina state, Nigeria. All the plant samples contained alkaloids, cardiac glycosides, flavonoids, saponins, tannins and terpenoids. Anthraquinones were found to be absent in Andropogon gayanus, Moringa oleifera and Pennisetum glaucum while phytosterol was absent in Pennisetum glaucum and Vernonia amygdalina. They were also found to contain nutrient compositions in varying amounts. Crotalaria retusa had the highest moisture (12.80%), crude lipid (13.50%) and crude fibre (21.76%) content. Momordica balsamina has the highest ash (13.76%) content. Pennisetum glaucum had the highest carbohydrate content (76.19%) and energy content (364.95%). Mineral analysis revealed that Andropogon gayanus, Momordica balsamina and Vernonia amygdalina showed equal phosphorus (0.3 ppm) contents. Crotalaria retusa showed the highest chromium (2.59 ppm) and copper (1.31 ppm) content. GC/MS analysis revealed that Momordica balsamina contained 99 phytoconstituents. With only 9,12-Octadecadienoic acid (Z,Z)- identified as the only phytoconstituents showing phytoestrogen activity. The indigenous knowledge about lactating mothers in Katsina state, Nigeria used medicinal plants to boost breast milk production is documented.

Capparis decidua is a valuable medicinal, xerophytic plant. We aim for the first time to compare the therapeutic potency of phytochemicals employing In-silico semblance, and ADME/Tox profiling studies. A solid computational strategy may ensure financial benefits in the phases of drug development, as well as innovation and reliable outcomes. This work investigated analgesic hypocholesterolemia, antidiabetic, and anti-inflammatory potency of Capparis decidua (CD) extract using MeOH:nH:EtAc (1:1:1, v:v:v) a solvent. Hence, GC–MS were used to characterize CD extract, and further, based on Ligand–Protein interaction, revealed Campesterol as a major inhibitor found to be possessing a higher interaction affinity (In kcal/mole; 2OYE; − 13.4, 1IR3; − 8.4, 3QNT; − 11.3, 3NUP; − 12.3, respectively), which leads to higher therapeutic potential. Further, various components were found to be bioactive including Neophytadiene, Palmitoleic acid, á-Sitosterol, Oleic acid, Rhodopin, 17-Pentatriacontene, Squalene, Piperine, 1-and, Eicosanol. Our results burlier proponent that Capparis decidua could help as a pain reliever, anti-inflammatory, and hypocholesterolemia problem, but more research is needed.

This research was conducted to evaluate the bioactive compounds content and biological activity of legume extract (mung bean (MB), job’s tears (JT), red kidney bean (RB) and black bean (BB)), sesame extract (white sesame (WS) and black sesame (BS)) and lemon basil seed extract (LB). The research also investigated the combined LB extract with kojic acid, acarbose and five rice volatile compounds against α-glucosidase, α-amylase and tyrosinase activity. The inhibitory activity of three enzymes and the extracts’ antioxidant capacity was closely related to the total phenolic and total flavonoid content. LB methanol extract (LB(ME)) had the highest total phenolic content, total flavonoid content and antioxidant capacity. LB water extract (LB(WE)) with the IC₅₀ of 0.04 ± 0.004 and 0.14 ± 0.01 mg/mL had the highest inhibition potent against α-glucosidase and tyrosinase enzymes. In comparison, LB(ME) with the IC₅₀ of 0.13 ± 0.01 mg/mL represented the highest inhibition potent on the α-amylase enzyme. Five rice volatile compounds with the α-glucosidase, α-amylase and tyrosinase inhibitory activity, low cytotoxicity and high anti-nitric oxide production were combined with LB extract to investigate the enzymatic inhibitory effect. The results indicated that LB(WE)/2-methoxy-4-vinylphenol and LB(WE)/2-pentyl furan had a synergic effect on α-glucosidase activity. We also investigated LB extract combined with acarbose and kojic acid against these enzymes. The results showed that LB(ME)/acarbose had the highest inhibition potent on α-glucosidase and α-amylase activity and LB(WE)/kojic acid had the highest on tyrosinase activity. Thus, these results suggest that using LB extract and the combined may be a useful therapeutic approach for diabetes and melanogenesis treatment.

To assess the effectiveness and safety of Korean medicine in managing postoperative pain. Retrospective, observational study. Medical records of 233 inpatients at a Korean medicine hospital who underwent Korean medicine treatments such as acupuncture, moxibustion, cuppping, bee-venom and herbal acupuncture, electrical therapy, herbal medicine, and carbon-beam therapy after musculoskeletal surgery were reviewed, and numeric ratings for pain were compared between baseline and follow-up. A correlation analysis between pain improvement and therapeutic factors was performed, and a regression analysis was conducted to determine the predictors of pain reduction. Serum laboratory test results at baseline and follow-up were subsequently compared to identify safety. Numeric rating scale scores for pain; presence of infection, drug-induced liver injury (DILI), and drug-induced kidney injury (DIKI) for safety. After Korean medicine treatment, the pain scores decreased from an average of 4.09 to 1.79 in the numeric rating scale (p < 0.001). Frequency of acupuncture therapy during hospitalization showed the strongest positive correlation with pain improvement (r = 0.341, p = 0.000) and was a predictor of pain reduction (adjusted R² = 0.145). Improvements in serum laboratory data to reference limits were observed. All kappa coefficient values, except for white blood cell (WBC) count, were between 0.3 and 0.7, indicating that the follow-up data matched the baseline data. The low kappa coefficient value of WBC count was due to the high ratio of outliers. One (0.43%) and no cases of DILI and DIKI were identified, respectively. Korean medicine could be used for managing pain after musculoskeletal surgery.