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Microscopica acta. Supplement最新文献

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Determination of cellular dry mass by automatic microinterferometry. 自动微干涉法测定细胞干质量。
Microscopica acta. Supplement
Pub Date : 1979-01-01
C Wientzeck, H Bröhl, J Bereiter-Hahn
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引用次数: 0
Automated morphometric estimation of liver steatosis. 肝脂肪变性的自动形态学估计。
Microscopica acta. Supplement
Pub Date : 1979-01-01
L Arnould, J P Cosyn, L Rondelez, P Courtoy
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引用次数: 0
[The statement of nuclear volume, DNA content and nuclear form in tissue sections (author's transl)]. [组织切片核体积、DNA含量和核形态的表述(作者译)]。
Microscopica acta. Supplement
Pub Date : 1979-01-01
H J Anton, R Thelen

15 days after amputation of the forelimb of young postmethamorphic Triturus vulgaris DNA-content and volume of the nuclei of the mesodermal blastema cells have been investigated in longitudinal sections of 2 microns in thickness by Feulgen-photometric and electronic determination of area. Discrimination of the DNA-synthesis phase was carried out using microautoradiography after "in vivo" labeling with tritiated thymidine. Volume and DNA-content of the nuclear sections have been found to be positive correlated. The DNA-synthesising part of the population shows characteristic differences to the mean value of volume and DNA-content of the remaining population. The factor circumference 2/area is suitable to characterize the differentiation degree of cells.

采用feulgen光度法和面积电子测定法，在2 μ m厚的纵向切片上测定了幼体变质后小黑麦前肢切除15 d后中胚芽细胞细胞核的dna含量和体积。在氚化胸腺嘧啶“体内”标记后，利用显微放射自显影技术进行dna合成相的鉴别。核切片的体积与dna含量呈正相关。种群的dna合成部分与其余种群的体积和dna含量平均值表现出特征性差异。因子周长2/面积适合用来表征细胞的分化程度。
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引用次数: 0
A new method of three-dimensional reconstruction of complicated structures by combining an automatic and interactive computer technique. 一种结合计算机自动与交互技术的复杂结构三维重建新方法。
Microscopica acta. Supplement
Pub Date : 1979-01-01
L Teckhaus, D W Lübbers, G Rager
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引用次数: 0
[Cell activation and nuclear volume (author's transl)]. [细胞活化和核体积(作者译)]。
Microscopica acta. Supplement
Pub Date : 1979-01-01
H J Anton, W Elsen

Freezing dried semithin sections (1 micron) coloured with nuclear fast red are suited for determination of volume and activation state of cells and nuclei by means of electronic image analysis. Additional investigations of the proteinsynthesis with help of autoradiographs after incorporation of tritiated amino acids affirm the informations. Magnification of the nucleus together with a simultaneous increase of the proteinsynthesis per unit volume characterize the Stratum basale near the amputation level as the most active area of the regeneration epidermis. The cell volume is correlated with the alterations of the nuclear volume. Determination of the mean relative volume of nuclei with aid of measurements of nuclei sections suffices to get informations about cell activity.

用核快红染色的冷冻干燥半薄片(1微米)适合于用电子图像分析方法测定细胞和细胞核的体积和激活状态。在加入了氚化氨基酸后，借助射线自显像对蛋白质合成的进一步研究证实了这些信息。细胞核的放大和单位体积蛋白质合成的同时增加表明，在截肢水平附近的基底层是再生表皮最活跃的区域。细胞体积与核体积的变化有关。通过对细胞核切片的测量来确定细胞核的平均相对体积足以获得有关细胞活动的信息。
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引用次数: 0
[Sampling techniques in quantitative morphology (author's transl)]. [定量形态学的采样技术(作者译)]。
Microscopica acta. Supplement
Pub Date : 1979-01-01
O Leder

Adequate methods of sampling are by far the most neglected procedures in stereological work. This is true of both theory and practice. Indeed, it is often forgotten that Delesse not only introduced the stereological method, but also specified the necessary conditions for its use. To help make good this deficiency methods of stereological sampling are reviewed. In particular, the ratio estimate is discussed and examples are given. Estimation of the percentages of each cell class in populations of blood cells and cells from vaginal smears have problems in common. In most cases the bias of the ratio estimate is trivial. The formula for approximate calculation of the standard error of the ratio estimate requires more sampling units than most people realize. In addition, in artifical smears, a more even distribution of the cells is recommended.

在立体学工作中，适当的抽样方法是迄今为止最容易被忽视的程序。理论和实践都是如此。的确，人们常常忘记，Delesse不仅介绍了立体学方法，而且还规定了使用立体学方法的必要条件。为了弥补这一缺陷，本文对立体学采样方法进行了综述。特别讨论了比率估计问题，并给出了实例。估计每一类细胞在血细胞和阴道涂片细胞群中的百分比有共同的问题。在大多数情况下，比率估计的偏差是微不足道的。比率估计标准误差的近似计算公式需要比大多数人认识到的更多的抽样单位。此外，在人工涂片中，建议细胞分布更均匀。
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引用次数: 0
[Proceedings of the Quantimet Symposium on Advances of Quantitative Image Analysis, Dortmund, 7-8 December 1978]. [定量图像分析进展的定量研讨会论文集，多特蒙德，1978年12月7-8日]。
Microscopica acta. Supplement
Pub Date : 1979-01-01
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引用次数: 0
Automatic micro-interferometry of tissue culture cells. 组织培养细胞的自动显微干涉测量。
Microscopica acta. Supplement
Pub Date : 1979-01-01
C Wientzeck, H Bröhl, J Bereiter-Hahn
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引用次数: 0
[Recent developments of instrument assisted chromosome analysis with Quantimet 720 (author's transl)]. [仪器辅助染色体分析Quantimet 720的最新进展]。
Microscopica acta. Supplement
Pub Date : 1979-01-01
W Malkusch

The system looks in a short time automatically for good metaphase spreadings. Two routines are described which evaluate the relocated metaphases in a semi-automatic way. One routine classifies human chromosomes, using the chromosome area and area ratio of the chromosome arms. The other routine classifies the chromosomes of the chinese hamster. In this routine statistical inputs for chromosome aberrations are possible.

系统在短时间内自动寻找良好的中期扩散。描述了以半自动方式评估重新定位中期的两个例程。一种方法是用染色体的面积和染色体臂的面积比对人类染色体进行分类。另一个程序是对中国仓鼠的染色体进行分类。在这个常规中，染色体畸变的统计输入是可能的。
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引用次数: 0
[Quantitative determination of the vascular volume of the cat carotid body under normoxia, hyperoxia, hypoxia and hypercapnia (author's transl)]. 【猫颈动脉体在常氧、高氧、低氧和高碳酸血症下血管体积的定量测定(作者译)】。
Microscopica acta. Supplement
Pub Date : 1979-01-01
E Seidl, H Acker, L Teckhaus

The ratio vascular volume to total volume was quantitatively analyzed in the cat's carotid body in dependence on the oxygen content of respired air and arterial pO2, respectively. Under hypoxia the vascular volume was 50% higher than under hyperoxia. This connection was ascertained after both perfusion fixation and immersion fixation.

定量分析猫颈动脉体血管容积与总容积的比值，分别依赖于呼吸空气含氧量和动脉pO2。缺氧时血管体积比高氧时大50%。灌注固定和浸泡固定均证实了这种联系。
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引用次数: 0
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