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Microscopica acta. Supplement最新文献

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Automated feature extraction from cineangiograms. Experience with routinely used hardwired system Contouromat. 从电影血管图像中自动提取特征。有常规使用硬连线系统Contouromat的经验。
Microscopica acta. Supplement
Pub Date : 1979-01-01
C J Slager, J H Reiber, G T Meester, J C Schuurbiers
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引用次数: 0
[Correlation of morphometric parameters with enzyme-activities in cases of liver diseases with fibrosis (author's transl)]. [肝纤维化患者形态计量参数与酶活性的相关性(作者译)]。
Microscopica acta. Supplement
Pub Date : 1979-01-01
N Dahrenmöller, A Roessner, G Pott, K M Müller

50 liver specimens with fibrotic liver disease were taken from current autopsy material and, after preparation, subjected to automatized image analysis by QUANTIMET 720 for quantitation of the connective tissues and their cellularity. In cell-free supernatants of liver tissue homogenates we determined the activities of N-acetyl-beta-glucosaminidase (beta-NAG, E.C. 3.2.1.30), a lysosomal glycosidase that is involved in proteoglycane metabolism of connective tissue. Beta-NAG-isoenzymes were demonstrated by electrofocusing in thin layers of polyamide gel. The results showed statistically significant correlations between beta-NAG activity and morphometrically assessed quantities of connective tissue, and of mesenchymal cells found in the latter or in the whole specimen, respectively. Demonstration of tissue or serum levels of beta-NAG or its isoenzymes provides a readily practicable method for assessing mesenchymal activities of the liver.

从目前的尸检材料中提取50例纤维化肝病肝脏标本，制备后用QUANTIMET 720进行自动图像分析，定量结缔组织及其细胞结构。在肝组织匀浆的无细胞上清中，我们测定了n -乙酰- β -氨基葡萄糖酶(β - nag, E.C. 3.2.1.30)的活性，这是一种参与结缔组织蛋白糖代谢的溶酶体糖苷酶。通过电聚焦在聚酰胺凝胶薄层中证实了β - nag -同工酶。结果显示，β - nag活性与结缔组织和间充质细胞的形态计量学评估数量之间具有统计学意义的相关性，分别在后者或整个标本中发现。证明组织或血清中β - nag或其同工酶的水平为评估肝脏间充质活性提供了一种简便可行的方法。
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引用次数: 0
Application of x-ray microanalysis to studies on distribution of diffusible ions in skeletal muscle tissue. x射线显微分析在骨骼肌组织中扩散离子分布研究中的应用。
Microscopica acta. Supplement
Pub Date : 1978-01-01
K Zierold, D Schäfer, J Gullasch
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引用次数: 0
X-ray microanalysis of frozen hydrated tissue sections as a physiological tool. 冷冻水合组织切片的x射线显微分析作为一种生理工具。
Microscopica acta. Supplement
Pub Date : 1978-01-01
A J Saubermann

Frozen hydrated tissue sections can be used as a physiological tool. The technique is a difficult one primarily because of the numerous possibilities for artifcatual interference. However, these problems are becoming better understood and the information which can be obtained through the use of these techniques is extremely promising.

冷冻的水合组织切片可以作为一种生理工具。这项技术是一项困难的技术，主要是因为人工干扰的可能性很大。然而，这些问题正在得到更好的理解，通过使用这些技术可以获得的信息是非常有希望的。
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引用次数: 0
Application of electron probe x-ray microanalysis to the detection of metal pollutants in freshwater zooplankton. 电子探针x射线微量分析在淡水浮游动物金属污染物检测中的应用。
Microscopica acta. Supplement
Pub Date : 1978-01-01
C Chassard-Bouchard, G Balvay
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引用次数: 0
The laser microprobe mass analyser (LAMMA): biomedical applications. 激光微探针质量分析仪:生物医学应用。
Microscopica acta. Supplement
Pub Date : 1978-01-01
R Kaufmann, F Hillenkamp, R Nitsche, M Schürmann, R Wechsung
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引用次数: 0
The nuclear microprobe, actual state of instrumentation and possible biomedical implications. 核微探针，仪器的实际状态和可能的生物医学意义。
Microscopica acta. Supplement
Pub Date : 1978-01-01
T B Pierce
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引用次数: 0
A BASIC-language program for dedicated energy-dispersive microanalysis of thin samples in TEM or STEM. 用于TEM或STEM中薄样品的专用能量色散微分析的基本语言程序。
Microscopica acta. Supplement
Pub Date : 1978-01-01
J C Russ

The program QTHIN combines those methods for processing energy-dispersive X-ray spectra and computing concentrations best suited to thin section microanalysis in TEM or STEM. The program is in BASIC, and so can be modified (for instance for unusual geometries, other matrices such as oxides, etc.). It is suitable for routine use on complex samples with peak overlaps, and for "not-so-thin" samples in which absorption is not negligible.

QTHIN程序结合了处理能量色散x射线光谱和计算浓度的方法，最适合于TEM或STEM中的薄片微分析。该程序是用BASIC编写的，因此可以进行修改(例如，对于不寻常的几何形状，其他矩阵，如氧化物等)。它适用于具有峰重叠的复杂样品的常规使用，以及吸收不可忽略的“不太薄”样品。
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引用次数: 0
Cathodoluminescence technique combined with blue light fluorescence microscopy for the investigation of the transport of molecules through the extravascular space of mammalians. 阴极发光技术结合蓝光荧光显微镜研究哺乳动物血管外空间的分子转运。
Microscopica acta. Supplement
Pub Date : 1978-01-01
W Bröcker, G Hauck, R Blaschke, H Schröer, G Pfefferkorn

The mechanisms of transport of molecules across the extravascular spaces represent an open problem. Vital microscopic results with marked proteins using the blue light luminescence technique indicate a movement of fluid along the connective tissue fibres. Furthermore, the findings suggest that the elastic fibres play an important role in this movement. It may also be possible to elucidate the extravascular pathway for molecules by means of the cathodoluminescence technique in the scanning electron microscope. The investigations were performed using the mesentery of mammalians anaesthetized by Nembutal. The biological material for cathodoluminescence applications was selected from animals without injection of luminescent dye as well as with animals following intravenous injection of marked proteins. Two techniques, critical point drying and freeze drying, were used in the preparation. Stained and unstained tissues were investigated in the scanning electron microscope with a special cathodoluminescence detector system. Simultaneously observations of the tissue surfaces were performed by means of the secondary electron or backscattered electron mode. The results obtained agree with the findings observed by the blue light fluorescence microscopy and confirm the concept of an extravascular fluid pathway along the elastic fibres in the tissue.

分子在血管外空间的运输机制是一个悬而未决的问题。使用蓝光发光技术对标记蛋白质的重要显微结果表明，液体沿着结缔组织纤维运动。此外，研究结果表明，弹性纤维在这种运动中起着重要作用。利用扫描电子显微镜的阴极发光技术也可以阐明分子的血管外通路。研究是在用Nembutal麻醉的哺乳动物的肠系膜上进行的。用于阴极发光应用的生物材料分别取自未注射荧光染料的动物和静脉注射标记蛋白的动物。采用临界点干燥和冷冻干燥两种技术制备。用专用的阴极发光检测系统在扫描电镜下观察染色组织和未染色组织。同时，通过二次电子或背散射电子模式对组织表面进行了观察。所获得的结果与蓝光荧光显微镜观察到的结果一致，并证实了沿组织中弹性纤维的血管外液体通路的概念。
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引用次数: 0
Chemical analysis of biological materials with the laser probe mass-spectrograph. 生物材料的激光探针质谱分析。
Microscopica acta. Supplement
Pub Date : 1978-01-01
J F Eloy
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引用次数: 0
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