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[On eruption of mandibular third molar after extraction of mandibular first or second molar]. [下颌第一或第二磨牙拔除后第三磨牙的萌出]。
K Yamabe, M Kouguchi, Y Watanabe, K Yamauchi

In the orthodontic practice, the first or second molars are extracted by reason of a necessity based on the treatment planning. In these cases, it is desirable that the third molar would be taken part in the masticatory function. This study was made to investigate the natural movement of the erupting third molars after the extraction of the first or second molar on the serial lateral cephalometric radiographs and orthopantomographs. Subjects were divided into three groups. The first is the group which the mandibular second molars were extracted. The second is the group which the first molars were extracted bringing about the successful eruption of the third molars. The third is the group which the first molars were extracted with resultant in the failure of the third molar eruption. In all cases, the orthodontic forces were not applied to the third molars. The results were as follows: 1. In the first group, all third molars were successfully erupted and a lot of the spaces after the extraction of second molars were utilized for the eruption of third molars. 2. In the second group, all third molars were successfully erupted and a lot of the spaces after the extraction of first molars were utilized for the mesial movement of the second molars. 3. In the third group, there were various patterns of the impaction of the third molars. It was suggested that the eruption of the third molar was related to the space distal to the second molar but it was not related to the anterio-posterior length of the mandibular body significantly.

在正畸实践中,第一或第二磨牙是根据治疗计划的需要拔除的。在这些情况下,第三磨牙将参与咀嚼功能是可取的。本研究通过连续侧位头颅x线片和正位断层片观察拔除第一或第二磨牙后萌出第三磨牙的自然运动情况。研究对象被分为三组。第一组是拔除下颌第二磨牙的组。第二组是拔除第一磨牙后,第三磨牙又成功长出的组。第三组是拔第一磨牙导致第三磨牙出牙失败的组。在所有病例中,正畸力均未应用于第三磨牙。实验结果如下:1.实验结果表明:第一组第三磨牙全部拔除成功,第二磨牙拔除后的大量空隙用于第三磨牙的拔除。2. 第二组第三磨牙全部拔除成功,第一磨牙拔除后的大部分空隙用于第二磨牙的近中活动。3.在第三组中,第三磨牙的嵌塞有不同的模式。提示第三磨牙的出牙与第二磨牙的远端间隙有关,而与下颌体的前后长度关系不明显。
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引用次数: 0
[Tooth movement of an impacted lower 2nd premolar in the root developmental stage--a follow-up radiographic observation on the root formation]. [根发育阶段阻生下第二前磨牙的牙齿运动——根形成的后续x线观察]。
N Ohya, T Ohya

A 9 year old girl presented an impacted lower right 2nd premolar which not only was in horizontal position, but its crown was directed lingually. As a lower right 2nd premolar was in such a position that normal eruption was impossible, the deciduous predecessor was extracted at 9 years of age, and then sufficient space had been created for the lower right 2nd premolar to be erupted, and the eruption of it was observed for about 2 years. 2 years later, radiographic indication showed that no spontaneous eruption of a lower right 2nd premolar was to be expected. At 11 years of age, surgical exposure and traction of a lower right 2nd premolar was attempted. When the lower right 2nd premolar was upright, its root formation was in the 1st stage of root developmental stages according to Moorrees et al. Follow up radiographic observations on surgical exposure and traction of a lower right 2nd premolar 3 years later revealed the following results: 1. The root development went on to amount to about 90 percent of the root formation of the other tooth concerned without the curvature of the root. 2. Lamina dura and periodontal space were normal. The continued root formation was narrowing the root apex and the pulpal tissue of apical foramina was continuous with the periodontal space surrounding the root. 3. Bone deposition occurred at the alveolar crest and the latter was recontoured normally. The interdental and inter-radicular trabecula were good. As based on these results, normal development of the root of a lower right 2nd premolar with normal periodontium can be anticipated. Clinically, the lower right 2nd premolar had completely erupted and was in normal position on the arch and in occlusion. Color, vitality and mobility were normal. Periodontal support was good. The above-mentioned findings give support to the possibility that "early treatment" enables the impacted tooth to correct.

一个9岁的女孩出现了右下第二前磨牙,不仅是在水平位置,而且它的冠指向舌。由于右下第二前磨牙处于不可能正常出牙的位置,因此在9岁时拔除了乳牙前体,为右下第二前磨牙的出牙创造了足够的空间,观察其出牙约2年。2年后,x线显示右下第二前磨牙没有自发性的爆发。11岁时,尝试手术暴露并牵引右下第二前磨牙。Moorrees等认为,右下第二前磨牙直立时,其根形成处于根发育阶段的第一阶段。3年后对右下第二前磨牙手术暴露和牵引的x线观察显示以下结果:在没有牙根弯曲的情况下,牙根的发育继续达到另一颗牙齿牙根形成的90%左右。2. 硬膜和牙周间隙正常。根的继续形成使根尖变窄,根孔的牙髓组织与根周围的牙周间隙连续。3.牙槽嵴发生骨沉积,牙槽嵴轮廓恢复正常。牙间和根间小梁良好。根据这些结果,可以预测右下第二前磨牙根部的正常发育,牙周组织正常。临床上右下第二前磨牙完全萌出,在牙弓上位置正常,咬合良好。颜色、活力和流动性正常。牙周支持良好。上述结果支持“早期治疗”可以使阻生牙得到矫正的可能性。
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引用次数: 0
[Clinical investigation of orthodontic treatment of palatally impacted canines, using ballista spring appliance]. [ballista弹簧矫治器治疗腭阻生犬的临床研究]。
S Umemura, T Yamaguchi, K Suginome, S Nishiguchi

Impacted maxillary caries are often treated in the orthodontic practice. However palatally impacted maxillary canines occur in a small. They require active orthodontic traction in those cases in which surgical exposure alone will not be enough to erupt. The purpose of this article is to describe useful orthodontic treatment for the palatally impacted caries by using the ballista spring appliance. Case 1: A girl 12 years 5 months of age had Angle Class 1 malocclusion with anterior cross bite and crowding teeth. Case 2: A woman 28 years 2 month of age had Angle Class 1 malocclusion with crowding teeth. The results were as follows: 1. After the impacted canines retracted and guided into the dental arch in correct position, the condition of the canines and tissues around have been stabilized. 2. The ballista spring appliance was effective in pulling the palatally impacted maxillary canines forward vertical direction.

上颌阻生龋齿在正畸治疗中是常用的治疗方法。然而上颌腭阻生的犬科动物很少。他们需要积极的正畸牵引在那些情况下,手术暴露将不足以爆发。本文的目的是描述使用balllista弹簧矫治器治疗腭阻生龋的有效方法。病例1:1例女孩,12岁5个月大,角1级错牙合,前交叉咬合,牙齿拥挤。病例2:一名28岁2个月的女性,有角1级错颌,牙齿拥挤。实验结果如下:1.实验结果表明:当阻生犬缩回并引导到牙弓正确位置后,犬和周围组织的状况稳定下来。2. 弹弓式矫治器对腭侧阻生上颌犬齿向前垂直方向牵引效果良好。
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引用次数: 0
Effect of micro-pulsed electricity on experimental tooth movement. 微脉冲电对实验牙齿运动的影响。
H Hashimoto

The purpose of this study was to determine whether a micro-pulsed electrical potential on the tooth surface could induce effective remodeling of the alveolar bone and enhance tooth movement. Twenty-eight cats were divided into 4 groups of 7 animals each for 1, 4, 7, and 14 days of treatment, respectively. A 60 gram orthodontic force was applied to distally tip the maxillary canines. In addition, the left canine only was subjected to a one hertz square wave form of 6 volts which induced a current of about 10 microA. Tooth movement and the amount of newly formed bone for the control and experimental sides were compared statistically using the Student's t test. Histochemical observations were also carried out. The results indicated that when electrical stimulus was applied to the surface of the canines in addition to mechanical force: 1) The periodontal tissue was affected. 2) The teeth moved more rapidly. 3) Wider areas of bone deposition and larger amounts of newly formed bone were observed on the tension side, and the amount of this newly formed bone increased with time. 4) More osteoblasts were observed on the tension side. 5) More tartrate-resistant acid phosphatase (TRACP) positive mononuclear and multinuclear cells were seen on the compression side. These results indicate that micro-pulsed electrical stimulation might play a role in the efficient remodeling of alveolar bone, and in inducing more rapid tooth movement.

本研究的目的是确定牙齿表面的微脉冲电势是否能诱导有效的牙槽骨重塑并增强牙齿的运动。将28只猫分为4组,每组7只,分别治疗1、4、7和14天。在上颌犬齿远端施加60克正畸力。此外,左犬只受到6伏特的一赫兹方波形式,产生约10微安的电流。采用学生t检验对对照组和试验组的牙齿运动和新骨形成量进行统计学比较。同时进行组织化学观察。结果表明:在机械力的基础上,对犬表面施加电刺激:1)对牙周组织产生影响。牙齿移动得更快了。3)张力侧骨沉积面积更广,新成骨量更大,且新成骨量随时间增加。4)张力侧成骨细胞较多。5)受压侧多见抗酒石酸酸性磷酸酶(TRACP)阳性的单核和多核细胞。这些结果表明,微脉冲电刺激可能在有效的牙槽骨重塑和诱导更快的牙齿运动中发挥作用。
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引用次数: 0
[The clinical study on occurrence of TMJ dysfunction in orthodontic patients]. 正畸患者颞下颌关节功能障碍发生的临床研究
H Tosa, T Imai, F Watanabe, M Sumori, T Tsuchida, I Matsuno, S Nakamura

Occlusal disharmony is considered to be the most significant factor of temporomandibular joint (TMJ) dysfunction, but the relationship between malocclusion and TMJ dysfunction has been unknown yet. The purpose of present study was to investigate the relationship between TMJ dysfunction and occlusal disharmony (eccentric occlusal contacts and functional shift of the mandible) in orthodontic patients. The subjects were 533 orthodontic patients, 209 males and 324 females, who were received at the Department of Orthodontics, School of Dentistry, Hokkaido University from April 1985 to March 1988. Their ages ranged from 8 to 38 years old. All were prior to orthodontic treatment. The results were as follows 1. The prevalence of TMJ dysfunction was 27.6%, 24.9% in males and 29.3% in females. The difference was insignificant between males and females. 2. The patients with TMJ dysfunction increased after 13-15 years old, corresponding to dental age IV A (Hellman's classification). 3. In patients with TMJ dysfunction, the highest frequency of symptom of TMJ dysfunction was joint sounds (77.6%), the second was pain (22.4%), the third was disturbance of mandibular movement (4.8%). The complication of those symptoms were compounded in 26.3% of the total patients. 4. The prevalence of TMJ dysfunction had no relation to specific malocclusion and maxillofacial morphologic characteristic. 5. Most patients with TMJ dysfunction had occlusal disharmony (95.9%). 6. Eccentric occlusal contacts were found most frequently in patients with open bite and functional shift of the mandible was found most frequently in patients with deep bite. From these findings, the prevalence of TMJ dysfunction was frequent in orthodontic patients, however the symptoms were objective and the patients were not aware of TMJ dysfunction. TMJ dysfunction had no relation to specific malocclusion but some kinds of occlusal disharmony were easy to occur in specific malocclusion and TMJ dysfunction was induced by those etiological factors.

咬合不和谐被认为是颞下颌关节(TMJ)功能障碍的最重要因素,但错颌与颞下颌关节功能障碍的关系尚不清楚。本研究旨在探讨正畸患者颞下颌关节功能障碍与咬合不协调(咬合接触偏心、下颌骨功能移位)的关系。研究对象为1985年4月~ 1988年3月在北海道大学牙科学院正畸科就诊的533例正畸患者,其中男性209例,女性324例。他们的年龄从8岁到38岁不等。所有患者均未接受正畸治疗。结果如下:1。颞下颌关节功能障碍的患病率为27.6%,男性为24.9%,女性为29.3%。男性和女性之间的差异不显著。2. 13-15岁后TMJ功能障碍患者增多,对应牙龄IV A (Hellman分级)。3.在颞下颌关节功能障碍患者中,出现颞下颌关节功能障碍的频率最高的是关节音(77.6%),其次是疼痛(22.4%),第三是下颌运动障碍(4.8%)。26.3%的患者合并上述症状。4. 颞下颌关节功能障碍的发生率与特定的错颌合和颌面部形态学特征无关。5. 颞下颌关节功能障碍患者以咬合不和谐为主(95.9%)。6. 偏颌接触多见于开咬患者,下颌功能移位多见于深咬患者。综上所述,正畸患者中颞下颌关节功能障碍的发生率较高,但其症状较为客观,患者对颞下颌关节功能障碍的意识较弱。颞下颌关节功能障碍与特异性错牙合无明显关系,但在特异性错牙合中易发生某些类型的咬合不和谐,这些病因因素诱发了颞下颌关节功能障碍。
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引用次数: 0
[Three-dimensional morphological analysis for craniofacial deformity]. [颅面畸形的三维形态学分析]。
I Matsuno, M Kawakami, M Yamamura, H Ishikawa, A Kudou, S Nakamura, O Takamichi, N Ohhata, Y Uchiyama, T Ohura

Morphological analysis of the craniofacial deformity has been performed by cephalometric radiography, but even with this method, it is difficult to analyze the cases with complicated deformity. The purpose of the present study is to develop the new 3-D CT morphological analyzing system in order to investigate complicated craniofacial deformity. Using the 67 landmarks, we established the 3 D line drawing method. Then the reproducibility of the landmarks were examined and the clinical cases were investigated. The measurement of landmarks were carried out 10 times by the same operator, and standard deviation of 67 landmarks were calculated. Some landmarks which were difficult to identify showed high value of standard deviation. However, it was almost same value between standard deviation of most of the landmarks and the CT system error. Therefore, location of landmarks in the system were found to be reproducible. This new analyzing system was performed in clinical cases with craniofacial deformity. In such cases, cranium deformity and complicated deformity such as twist-form arrangement of cranium, maxilla and mandible were detected. The 3-D CT morphological analysis is effective for analyzing severe craniofacial deformity which was impossible to recognize with conventional cephalometric analysis.

颅面畸形的形态分析已通过头颅x线摄影进行,但即使使用这种方法,也难以分析复杂畸形的病例。本研究的目的是开发新的三维CT形态分析系统,以研究复杂的颅面畸形。利用67个地标,建立了三维线条绘制方法。然后检查标记的再现性,并对临床病例进行调查。同一操作员对地标进行了10次测量,计算了67个地标的标准差。一些难以识别的标志显示出较高的标准差值。然而,大多数地标的标准差与CT系统误差之间的值几乎相同。因此,发现系统中地标的位置是可重复的。这种新的分析系统应用于颅面畸形的临床病例。在这些病例中,检测到头盖骨畸形和复杂畸形,如头盖骨、上颌骨和下颌骨的扭曲排列。三维CT形态分析可以有效地分析严重的颅面畸形,而传统的头颅测量分析无法识别。
{"title":"[Three-dimensional morphological analysis for craniofacial deformity].","authors":"I Matsuno,&nbsp;M Kawakami,&nbsp;M Yamamura,&nbsp;H Ishikawa,&nbsp;A Kudou,&nbsp;S Nakamura,&nbsp;O Takamichi,&nbsp;N Ohhata,&nbsp;Y Uchiyama,&nbsp;T Ohura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Morphological analysis of the craniofacial deformity has been performed by cephalometric radiography, but even with this method, it is difficult to analyze the cases with complicated deformity. The purpose of the present study is to develop the new 3-D CT morphological analyzing system in order to investigate complicated craniofacial deformity. Using the 67 landmarks, we established the 3 D line drawing method. Then the reproducibility of the landmarks were examined and the clinical cases were investigated. The measurement of landmarks were carried out 10 times by the same operator, and standard deviation of 67 landmarks were calculated. Some landmarks which were difficult to identify showed high value of standard deviation. However, it was almost same value between standard deviation of most of the landmarks and the CT system error. Therefore, location of landmarks in the system were found to be reproducible. This new analyzing system was performed in clinical cases with craniofacial deformity. In such cases, cranium deformity and complicated deformity such as twist-form arrangement of cranium, maxilla and mandible were detected. The 3-D CT morphological analysis is effective for analyzing severe craniofacial deformity which was impossible to recognize with conventional cephalometric analysis.</p>","PeriodicalId":76235,"journal":{"name":"Nihon Kyosei Shika Gakkai zasshi = The journal of Japan Orthodontic Society","volume":"49 4","pages":"291-301"},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13288002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Multivariate analysis for the study of craniofacial structure. Selection of parameters on using the principal component analysis]. 颅面结构研究的多变量分析。参数选择上采用主成分分析]。
K Sato, T Saito, H Mitani

The present study was undertaken to discuss the effects of parameters on using the principal component analysis for the evaluation of the craniofacial structures. Materials consisted of lateral roentgenocephalograms of 100 adult Japanese females. They were divided into three groups by ANB angle. Linear and angular measurements were selected as parameters to apply the principal component analysis, and scattergrams made by the first and second principal component were compared. The results of the analysis were varied by the parameters. Especially there was a great difference between the real size and the value corrected by isometric method on scattergrams made by the first and second components. Results indicated the importance of using appropriate parameters for the research purpose.

本研究旨在探讨主成分分析对颅面结构评价的影响。材料包括100名成年日本女性的侧位x线头像。按ANB角度分为三组。选取线性测量和角度测量作为参数进行主成分分析,比较第一主成分和第二主成分的散射图。分析结果因参数的不同而不同。特别是在第一和第二分量的散射图上,实际尺寸与等距法校正的值相差很大。结果表明,使用合适的参数对研究目的的重要性。
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引用次数: 0
[Importance of posterior tooth-to-denture base discrepancy in the development of skeletal open-bite malocclusion]. [后牙-义齿基托差异在骨性开咬错发展中的重要性]。
S Sato, K Sasaguri, S Kamoi, M Goto, Y Suzuki

This paper describes the relationship between the development of skeletal open-bite malocclusion and the tooth-to-denture base discrepancy. The cases which have a severe skeletal open-bite malocclusion are presented to evaluate the causing factor of the anterior open-bite. The anterior open-bite was associated with inferiorly positioned maxillary molars caused by the squeezing out effect of the tooth-to-denture base discrepancy, especially those in posterior part of dentition (posterior discrepancy), which provided a less steep maxillary occlusal plane in the denture frame. It was suggested that the posterior discrepancy induced a descending movement of the posterior teeth followed by a change of the occlusal plane and this effect of the posterior discrepancy was important factor in developing anterior open-bite malocclusion.

本文介绍了骨性开咬错的发生与牙基与义齿基差的关系。本文介绍了严重骨开合错的病例,探讨了引起前牙开合的因素。由于牙基差的挤压作用,上颌磨牙位置偏下,尤其是牙列后段(后差),使得上颌牙合平面在义齿架内的倾斜度较低。结果表明,后牙差引起后牙的下降运动,从而引起咬合平面的改变,这是导致前牙开合错的重要因素。
{"title":"[Importance of posterior tooth-to-denture base discrepancy in the development of skeletal open-bite malocclusion].","authors":"S Sato,&nbsp;K Sasaguri,&nbsp;S Kamoi,&nbsp;M Goto,&nbsp;Y Suzuki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper describes the relationship between the development of skeletal open-bite malocclusion and the tooth-to-denture base discrepancy. The cases which have a severe skeletal open-bite malocclusion are presented to evaluate the causing factor of the anterior open-bite. The anterior open-bite was associated with inferiorly positioned maxillary molars caused by the squeezing out effect of the tooth-to-denture base discrepancy, especially those in posterior part of dentition (posterior discrepancy), which provided a less steep maxillary occlusal plane in the denture frame. It was suggested that the posterior discrepancy induced a descending movement of the posterior teeth followed by a change of the occlusal plane and this effect of the posterior discrepancy was important factor in developing anterior open-bite malocclusion.</p>","PeriodicalId":76235,"journal":{"name":"Nihon Kyosei Shika Gakkai zasshi = The journal of Japan Orthodontic Society","volume":"49 4","pages":"322-30"},"PeriodicalIF":0.0,"publicationDate":"1990-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13288005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Study on the simulation in planning for orthognathic surgery by using a personal computer]. 个人计算机模拟正颌手术规划的研究
K Terada, I Saitoh, K Hanada

This study was made in order to develop and evaluate three-dimensional simulation programs in planning for orthognathic surgery by using microcomputer system. Microcomputer system is composed of CPU (NEC, PC-9801), high resolutional color display, color printer, image scanner, and MOUSE. This simulation is composed of four procedures. 1. Data input Three-dimensional coordinate data on tracing obtained from CT films is inputted. One data file is made from one outline of structures. 2. Three dimensional reconstruction A control file is made to arrange data files constructing structures. 3. Simulation This procedure is composed of cutting and movement. The horizontal projection of mandible is displayed. After cutting simulated orthognathic surgery is designed, new data files and new control file are made. A movement consists of parallel translation and rotation. A movement data is combined with movements designed on each of three projections. 4. Three-dimensional graphic display Three-dimensional graphics of the mandible before and after a simulation for orthognathic surgery are displayed by perspective views with treated hidden lines and accurate pictures (COSMOZONE 2SA, NIKON). These simulation programs were carried out with the asymmetrical patient. The simulation was supposed that the operation was combined with sagittal splitting osteotomy of the right mandibular ramus and body ostectomy of the left lower first premolar part. Three-dimensional graphics of the mandible before and after the simulation were presented. Its three-dimensional mandibular position and form after orthognathic surgery were predicted. These three-dimensional simulation programs may be important procedure, when a treatment planning in asymmetrical prognathic case is discussed.

本研究是为了利用微机系统开发和评价三维模拟程序在正颌手术规划中的应用。微机系统由中央处理器(NEC, PC-9801)、高分辨率彩色显示器、彩色打印机、图像扫描仪和鼠标组成。该仿真由四个步骤组成。1. 数据输入输入从CT胶片上获得的跟踪三维坐标数据。一个数据文件由一个结构大纲组成。2. 三维重建生成一个控制文件来安排构造结构的数据文件。3.该程序由切割和移动两部分组成。显示下颌骨的水平投影。模拟正颌手术设计完成后,制作了新的数据文件和新的控制文件。运动由平行平移和旋转组成。运动数据与三个投影上设计的运动相结合。4. 三维图形显示模拟正颌手术前后的下颌骨三维图形通过处理过的隐线透视视图和精确图片显示(COSMOZONE 2SA, NIKON)。这些模拟程序是在不对称患者身上进行的。模拟拟联合右侧下颌支矢状劈裂截骨术和左侧下第一前磨牙部分体骨切除术。给出了模拟前后下颌骨的三维图形。预测其在正颌手术后的三维下颌位置和形态。这些三维模拟程序在讨论不对称前凸病例的治疗方案时可能是重要的程序。
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引用次数: 0
[A study to establish the model of transseptal fibers in vitro by human gingival fibroblast]. [利用人牙龈成纤维细胞体外建立跨隔纤维模型的研究]。
S Tenshin, M Tsuchihasi, K Sou, K Sumitani, K Yamaguchi, T Kawata

The present study was attempted to establish the model of transseptal fibers in vitro to clarify the remodeling mechanisms of the fibers during orthodontic treatment. Human premolars were transversally cut. The thickness of films was 150 microns and a pair of dental rings was placed at intermediate of 500 microns in tissue culture dishes. Human gingival fibroblasts were seeded in these dishes and cultured. Culturing of these cells were changed at 1, 5, 10 and 20 days and used to histological observation. The following results were obtained: 1. It was observed that human gingival fibroblasts and fibrillar materials oriented approximately the surfaces of sliced teeth and extended the process of cells along the direction from sliced teeth to another one. The bridges of cells and fibers between two sliced teeth were completed after 10 days culturing. 2. The fibrillar materials were examined by TEM and bundles constructed from banded fibrils which each band was 60 nm in diameter. The red fibers were stained with Van Gieson's stain method and so many red fibers filled in the space between the two sliced teeth. This suggests that the most of fibrillar materials were collagen fibers formed from human gingival fibroblasts. The structural materials were assumed to be resembled to transseptal fibers in vivo. This culture system is usefull to study the remodeling mechanisms of transseptal fibers in tooth movement.

本研究试图通过体外建立跨隔纤维模型,阐明正畸治疗过程中纤维的重塑机制。人类的前磨牙是横向切割的。膜的厚度为150微米,在500微米的中间位置放置一对牙环。人牙龈成纤维细胞在这些培养皿中播种并培养。分别于第1、5、10、20天进行细胞培养,并进行组织学观察。得到了以下结果:1。观察到人牙龈成纤维细胞和原纤维材料大致沿牙片表面取向,细胞沿牙片方向向另一个方向延伸。培养10天后,两颗切牙之间的细胞和纤维完成桥接。2. 用透射电镜对纤维材料进行了检测,发现纤维束由带状原纤维构成,每条带状原纤维的直径为60 nm。用Van Gieson染色法对红色纤维进行染色,许多红色纤维填满了两颗切下的牙齿之间的空隙。这表明大部分纤维材料是由人牙龈成纤维细胞形成的胶原纤维。结构材料被认为类似于体内的隔隔纤维。该培养体系有助于研究牙运动中隔纤维的重塑机制。
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引用次数: 0
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Nihon Kyosei Shika Gakkai zasshi = The journal of Japan Orthodontic Society
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